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1.
Bemisia tabaci (Gennadius) adults were collected from poinsettia plants (Euphorbia pulcherrima) in retail nurseries in Cd. Obregon and Navojoa, Sonora, Mexico. A single field sample was collected from broccoli plants
in Obregon, Sonora. Both adult whitefly and immature instars were observed on infested leaves. Whiteflies were identified
asB. tabaci using morphological characters of the pupae to distinguish them from the greenhouse whitefly; and to specific biotype, by
molecular analysis using the mitochondrial cytochrome oxidase I (mtCOI) sequence. Phylogenetic analysis of mtCOI sequences
indicated that poinsettias were colonized both by the Q and the B biotype. The Q biotype was found only on poinsettia plants,
and one poinsettia sample was infested with both the Q and the B biotype. The B biotype alone was associated with the field-collected
broccoli sample analyzed in the study. A more extensive survey is required to determine the extent of the distribution of
the Q biotype in Mexico, particularly where ornamental plants are transported from central to northern Mexico. Such plants
could serve as the source of the Q biotype, which has been reported to be highly resistant to insecticides including the neo-nicotinoids
that are widely used to control the B biotype in much of Mexico. This is the first report of the Q biotype in Mexico.
http://www.phytoparasitica.org posting May 2, 2007. 相似文献
2.
James A. Bethke Frank J. Byrne Greg S. Hodges Cindy L. McKenzie Robert G. Shatters Jr. 《Phytoparasitica》2009,37(1):61-64
Bemisia tabaci (Gennadius) adults and immatures were collected from poinsettia plants at two commercial production greenhouses in Guatemala
during an invited tour to observe IPM practices within the facilities. Despite extensive scouting, only low numbers of insects
were collected from vegetable, weed and wild ornamentals species located close to these facilities. Prior to molecular and
biochemical analyses, whitefly immatures were initially identified as B. tabaci using morphological characters of the pupae to distinguish them from the greenhouse whitefly. The biotype status of adults
and immatures was then established using esterase isozyme patterns and MTCO1 sequencing. The Q biotype was the only biotype
found on commercially grown poinsettia plants. The previously recorded B biotype was observed outside the greenhouse facilities
on Lactuca spp., Hibiscus spp. and Euphorbia spp. (wild poinsettia). The New World biotype was observed on wild poinsettia and field-grown beans (Phaseolus spp.). This is the first report of the Q biotype in Guatemala, and serves notice of the need for greater vigilance in the
management of whiteflies on poinsettia mother stock used as a source of cuttings for export to the USA. 相似文献
3.
为明确L-阿拉伯糖对B型和Q型烟粉虱毒性及其取食行为的影响,调查了饲喂含有L-阿拉伯糖人工饲料后烟粉虱的死亡率,利用刺吸电位技术(EPG)记录其取食行为,并观察了饲喂后其在人工饲料膜上的刺孔数量及直径。结果表明,B型和Q型烟粉虱的校正死亡率均随L-阿拉伯糖浓度及饲喂时间的增加而升高;在3种浓度下,B型烟粉虱校正死亡率均显著高于Q型烟粉虱;在5%、10%浓度下,Q型烟粉虱校正死亡率分别在第5天和第3天达100%,B型烟粉虱分别在第3天和第2天达到100%;5%L-阿拉伯糖对B型烟粉虱取食行为影响比Q型大;在5%浓度下,B型和Q型烟粉虱在膜上的刺孔数量总体少于对照组。研究表明,L-阿拉伯糖对烟粉虱具有杀虫活性,且对B型和Q型烟粉虱的毒性效果不同。 相似文献
4.
Phylogenetic analysis using ITS1 and CO1 nucleotide sequences has revealed six major races ofBemisia tabaci in the world, including three major indigenous races in the Asia-Pacific region,viz., B. tabaci (Asia),B. tabaci (Bali) andB. tabaci (Australia), but the status of a large collection of genotypes in this region remains unresolved. The ITS1 sequences of representative
whitefly samples collected from around China were determined in this study. These sequences and other homologous sequences
retrieved from GenBank were then used to conduct a phylogenetic analysis. The results demonstrated that the whiteflies collected
in China were split genetically into four groups, where at least five genetic races were revealed,i.e., B biotype (SDLe, XJEp, XJAt, HNNt, BJIb, GDEp, XJGh, GDHrs, XJSm and SHEp), Bali group (ZJGh), M biotype (Hainan1), G biotype
(GXCm) and Asian H/K group (FJIb, GDCv), although the Asian H/K group with low bootstrap score remains unresolved. Of all
genetic races, the B biotype is the most extensively distributed. In the dendogram, the J biotype, L biotype and Q biotype
cluster together and form a sister clade to the B biotype. The data indicate that extensive migration ofB. tabaci has taken place in Asian countries. The populations ZJGh, FJIb, GDCv, GXCm and Hainanl collected in China might have originated
there, but the possibility that they were introduced from elsewhere cannot be excluded at this point. Using PyR from Israel
as a reference Q biotype, the random amplified polymorphic DNA banding patterns of SDLe, XJEp, XJAt and HNNt were shown to
be consistent with that of the Q biotype, which indicated that the four local whitefly populations identified as the B biotype
based on ITS1 sequences were closely related to the Q biotype.
http//www.phytoparasitica.org posting Sept. 12, 2006. 相似文献
5.
为有效控制日光温室番茄褪绿病毒病,于2014—2015年通过RT-PCR检测方法研究了济南市日光温室番茄褪绿病毒(Tomato chlorosis virus,ToCV)的发生规律、其与Q型烟粉虱Bemisia tabaci种群动态的关系及防虫网对该病毒病的防控效果。结果表明,春季日光温室番茄植株上Q型烟粉虱成虫数量呈增长趋势,5月下旬最高达到0.10头/叶,秋季日光温室番茄植株上Q型烟粉虱成虫数量9月上旬达最高7.42头/叶,后逐渐下降;日光温室Q型烟粉虱带毒率随着定植时间的延长而逐渐上升,之后维持相对稳定状态,即春季为20.00%~24.14%,秋季为30.00%~40.00%。日光温室ToCV发生与Q型烟粉虱成虫数量和带毒率密切相关,春季番茄最高发病率为12.00%;秋季番茄植株最高发病率为93.02%。番茄育苗和生长期用100目防虫网隔离可显著降低番茄植株带毒率。因此,秋季是日光温室ToCV防控关键期,覆盖防虫网阻隔烟粉虱可有效防治ToCV,推荐在日光温室使用。 相似文献
6.
为明确江苏地区Q型烟粉虱的遗传多样性及其入侵来源,基于mt DNA COI基因序列,对2010、2011年采自江苏13个市的Q型烟粉虱种群进行了单倍型分析。结果显示,江苏地区Q型烟粉虱有4个单倍型,分别为单倍型Q1、Q2、Q3、Q4,不同单倍型的分布和发生频率不同,其中单倍型Q2是13个地理种群的共享单倍型,2010、2011年的发生频率均超过50%;单倍型Q1和Q3分别是部分地理种群的共享单倍型,发生频率较低;单倍型Q4仅在扬州种群中出现,发生频率最低;单倍型Q1和Q3亲缘关系较近,单倍型Q2和Q4亲缘关系较近,表明各地理种群间既有一定的基因交流,也存在一定程度的遗传分化;系统发育分析表明,江苏地区的Q型烟粉虱可能来源于西部地中海地区,与日本的Q型烟粉虱具有相同的入侵来源。 相似文献
7.
The biotype status of samples of the whiteflyBemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) collected from several field and greenhouse sites in Israel during 1999–2000 was determined
by polyacrylamide gel electrophoresis (PAGE) for general esterases, and by RAPD-PCR using primers of arbitrary sequence. Results
of this survey provide the first published evidence for the occurrence of theB. tabaci Q biotype, alongside the more widely distributed B biotype. Based on the collected samples, it appears that both the B and
Q biotypes are present in Israel, and that field populations consist of a mixture of the two biotypes. A possible link betweenB. tabaci biotypes and insecticide resistance is discussed.
Contribution no. 508/02 from the Inst. of Plant Protection, ARO, The Volcani Center, Bet Dagan, Israel.
http://www.phytoparasitica.org posting Dec. 5, 2002. 相似文献
8.
为明确烟粉虱Bemisia tabaci取食感染番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)的番茄植株后,其体内的芳香基硫酸酯酶B基因(arylsulfatase B,ARSB)是否能够做出应答反应,基于Q型烟粉虱基因组数据克隆得到ARSB基因cDNA全长,采用生物信息学方法分析其序列特征,并通过实时荧光定量PCR技术测定ARSB基因在Q型烟粉虱不同发育阶段、不同组织及携带TYLCV前后的表达量变化情况。结果显示:Q型烟粉虱ARSB基因的cDNA全长为1 731 bp,编码576个氨基酸,分子量为64.89 kD,具有ARSB的保守结构域。ARSB基因在Q型烟粉虱不同发育阶段均有表达,在卵期表达量最高,成虫期表达量最低;该基因在Q型烟粉虱头胸部的表达量显著高于腹部;Q型烟粉虱获取TYLCV 72 h后其体内ARSB基因表达量显著提高。表明ARSB基因在Q型烟粉虱不同龄期、不同组织内存在差异表达,并可能参与Q型烟粉虱对TYLCV的响应和传毒过程。 相似文献
9.
BACKGROUND: Resistance to numerous insecticide classes in Bemisia tabaci Gennadius has impaired field control efficacy in south‐eastern China. The biotype and resistance status of B. tabaci collected from these areas was investigated. RESULTS: Two different biotypes of B. tabaci (B‐biotype and Q‐biotype) were detected in south‐eastern China, and the samples collected from geographical regions showed a prevalence of the Q‐biotype and the coexistence of B‐ and Q‐biotypes in some regions. Moderate to high levels of resistance to two neonicotinoids were established in both biotypes (28–1900‐fold to imidacloprid, 29–1200‐fold to thiamethoxam). Medium to high levels of resistance to alpha‐cypermethrin (22–610‐fold) were also detected in both biotypes. Four out of 12 populations had low to medium levels of resistance to fipronil (10–25‐fold). Four out of 12 populations showed low levels of resistance to spinosad (5.7–6.4‐fold). All populations tested were susceptible to abamectin. CONCLUSION: The Q‐biotype B. tabaci is supplanting the B‐biotype which used to be ubiquitous in China. Field populations of both B‐ and Q‐biotypes of B. tabaci have developed high levels of resistance to imidacloprid and thiamethoxam. Abamectin is the most effective insecticide against adult B. tabaci from all populations. Copyright © 2010 Society of Chemical Industry 相似文献
10.
Esther Fernández Carolina Grávalos Pedro Javier Haro Dina Cifuentes Pablo Bielza 《Pest management science》2009,65(8):885-891
BACKGROUND: Bemisia tabaci Gennadius Q‐biotype has readily developed resistance to numerous insecticide classes. Studies in the Mediterranean area are needed to clarify the resistance status and cross‐resistance patterns in this invasive whitefly biotype. The levels of resistance in nymphs of seven strains of B. tabaci Q‐biotype from south‐eastern Spain to representative insecticides were determined. RESULTS: Six populations had low to moderate levels of resistance to azadirachtin (0.2‐ to 7‐fold), buprofezin (11‐ to 59‐fold), imidacloprid (1‐ to 15‐fold), methomyl (3‐ to 55‐fold), pyridaben (0.9‐ to 9‐fold), pyriproxyfen (0.7‐ to 15‐fold) and spiromesifen (1‐ to 7‐fold), when compared with a contemporary Spanish Q‐biotype reference population (LC50 = 2.7, 8.7, 15.2, 19.9, 0.34, 20.9 and 1.1 mg L?1 respectively). A single population collected from a greenhouse subject to intensive insecticide use exhibited generally higher resistance levels to the same array of compounds (31‐, 1164‐, 3‐, 52‐, 9‐, 19‐ and 3‐fold respectively). Pyridaben and spiromesifen were extremely effective against nymphs of all strains, with LC50 values significantly below recommended application rates. CONCLUSION: In contrast to previous reports, high rates of efficacy exist for numerous insecticide classes against B. tabaci Q‐biotype populations in these intensive agricultural regions of south‐eastern Spain. This probably reflects the recent and significant reductions in exposure that have resulted from a wider uptake of IPM technologies and strategies. However, the continued presence of resistance genes also suggests that a reversion to levels of high insecticide exposure could result in a rapid selection for resistance. Copyright © 2009 Society of Chemical Industry 相似文献
11.
The recent upsurgence ofBemisia tabaci (Genn.) as an important insect pest and vector ofTomato yellow leaf curl virus (TYLCV) is directly linked to serious damage to tomato crops grown throughout Japan. The molecular genetic identification
and phylogenetic relationships of 12B. tabaci populations collected from representative locations in Japan were determined based on the mitochondrial cytochrome oxidase
I (mtCOI) sequence. Phylogenetic analysis of the whitefly mtCOI sequence indicated that both the invasive B and Q biotypes
now occur in Japan. The Q biotype was found at four locations: Mihara in Hiroshima, Nishigoshi in Kumamoto, Miyanojo and Okuchi
in Kagoshima prefectures; the remaining eight collections were identified as the B biotype. This is the first report of the
introduction of Q biotype in Japan.
http://www.phytoparasitica.org posting July 21, 2006. 相似文献
12.
为探究烟粉虱对不同番茄品种的适生性差异,于温室条件下采用生态测定和生化方法,研究了Q型烟粉虱在不同番茄品种上的生物学参数及不同番茄品种叶片营养物质和次生物质含量的差异,并利用"Y"型嗅觉仪测定了Q型烟粉虱成虫对不同番茄品种的行为反应。结果表明,Q型烟粉虱在供试5个不同番茄品种上的存活率、产卵量及种群生命表参数均存在显著差异,其存活率、产卵量、净增殖率和内禀增长率均以在绿肩2号上最高,分别为80.43%、314.00、42.21和0.12,在精品淑娇上最低,分别为41.58%、93.33、12.05和-0.10。不同番茄品种的营养物质和次生物质含量差异显著,可溶性蛋白质和叶绿素含量均以绿肩2号最高,分别为4.13、2.50 mg/g,总黄酮和总酚含量以金陵红玉最高,分别为9.60、9.04 mg/g。行为反应结果显示Q型烟粉虱对绿肩2号的选择性较强,与苏红2003相比,选择率为61.5∶38.5;对精品淑娇最弱,与绿肩2号相比,选择率为41.3∶58.7。 相似文献
13.
BACKGROUND: B and Q biotypes of the whitefly, Bemisia tabaci (Gennadius), are generally regarded as the most significant given their global distribution and strong resistance to insecticides. Since these biotypes can coexist and differ markedly in their insecticide resistance profiles, a rapid but reliable means of discriminating between them would be a valuable complement to resistance monitoring and management programmes. Recently, PCR‐based methods have been developed to determine the biotype status of B. tabaci populations. However, these require post‐amplification procedures, which increase time and labour. RESULTS: The authors have developed an allelic discrimination real‐time PCR assay using fluorescent dye‐labelled probes to distinguish the B and Q biotypes. The assay targets a single nucleotide polymorphism (SNP) in the mitochondrial cytochrome oxidase I (mtCOI) gene. To evaluate the assay, DNA was extracted from individual whiteflies of six known biotype strains, and all scored correctly as either a B or Q biotype. As further validation, 72 individuals from field samples collected in different parts of the world were also tested by the assay. No failed reactions were observed, with all 72 samples scoring clearly as either the B or Q biotype. CONCLUSION: The development of this rapid and high‐throughput assay has important potential for routine monitoring of B and Q biotypes on ornamental plants and for the screening of B. tabaci populations in countries where these biotypes are not yet established. Copyright © 2007 Society of Chemical Industry 相似文献
14.
The spatial dispersal patterns ofTomato yellow leaf curl virus (TYLCV) disease vectored by the B and Q biotypes of the whiteflyBemisia tabaci in tomato glasshouses were compared. Tomato plants were arranged in glasshouses and TYLCV-infected plants were placed in
the center of each plot. Adult whiteflies of each biotype were released onto TYLCV-infected plants and the insects were then
freely dispersed in the glasshouses under high or moderate temperature conditions. The abundance and spatial distribution
of dispersed whiteflies did not differ between the B and Q biotypes in tomato glasshouses. The disease incidence and dispersion
of TYLCV as a result of short-distance movement of the whiteflies were also similar between the two biotypes, although on
several investigation dates there was a tendency for the disease incidence caused by the B biotype to be slightly greater
than that caused by the Q biotype. These results demonstrated that the aspects of spatial spread of TYLCV vectored by the
B and Q biotypes ofB. tabaci in tomato glasshouses are similar.
http://www.phytoparasitica.org posting Dec. 11, 2007. 相似文献
15.
Soon‐Il Kim Song‐Hwa Chae Hee‐Sung Youn Seong‐Hum Yeon Young‐Joon Ahn 《Pest management science》2011,67(9):1093-1099
BACKGROUND: The contact + fumigant toxicity of 92 plant essential oils and control efficacy of 18 experimental spray formulations containing nine selected essential oils (0.5 and 0.1% sprays) and six commercial insecticides to females from B‐ and Q‐biotypes of Bemisia tabaci were evaluated using vapour‐phase mortality and spray bioassays. RESULTS: Garlic and oregano (LC50, 0.15 mL cm?3) were the most toxic oils against B‐ and Q‐biotype females. Strong fumigant toxicity to both biotype females was also obtained from catnip, cinnamon bark, clove bud, clove leaf, davana, savory and vetiver Haiti oils (LC50, 0.17–0.48 mL cm?3). The 0.5% sprays of these oils (except for thyme red oil) resulted in 90–100% mortality against both biotype females. Only garlic applied as 0.1% spray provided 100% mortality. Spinosad 100 g L?1 suspension concentrate (SC) treatment resulted in 92 and 95% mortality against both biotype females, whereas acetamiprid 80 g L?1 wettable powder (WP), imidacloprid 80 g L?1 SC, thiamethoxam 100 g L?1 water‐dispersible granule (WDG) and pyridaben 200 g L?1 WP treatments resulted in 89–100% mortality against B‐biotype females only. CONCLUSION: In the light of global efforts to reduce the level of highly toxic synthetic insecticides in the agricultural environment, the essential oils described, particularly garlic, cinnamon bark and vetiver Haiti, merit further study as potential insecticides for the control of B. tabaci populations as fumigants with contact action. Copyright © 2011 Society of Chemical Industry 相似文献
16.
为明确山东寿光地区Q型烟粉虱对番茄褪绿病毒(Tomato chlorosis virus,To CV)感病流行的影响及其传毒特性,于2014年调查了该地区设施番茄上烟粉虱种群动态与To CV发病情况,利用特异引物对烟粉虱体内To CV进行了RT-PCR检测;并在室内测定了带毒Q型烟粉虱取食时间和种群数量对To CV感病株率的影响。结果表明,在番茄发病植株上采集的烟粉虱种群体内可检测到To CV;春茬番茄To CV发病株率随烟粉虱种群数量增加而逐渐升高,4—6月是To CV发生高峰期,6月22日发病株率达100%;秋茬番茄烟粉虱种群数量从10月下旬明显下降,而To CV发病株率升高,11月12日发病株率达100%;室内试验表明,To CV感病株率随着带毒Q型烟粉虱数量与取食时间的增加而明显升高。研究表明,Q型烟粉虱能有效传播To CV,且其种群数量对To CV发病株率存在显著影响,可通过防控烟粉虱以控制To CV的危害。 相似文献
17.
18.
Tim Jürgens Celeste C. Linde Bruce A. McDonald 《European journal of plant pathology / European Foundation for Plant Pathology》2006,115(2):223-233
Restriction fragment length polymorphism (RFLP) markers were used to assess the genetic structure of populations of Mycosphaerella graminicola collected from wheat fields. A total of 585 isolates representing 10 field populations were sampled from Iran, Argentina and Australia. The genetic structure of M. graminicola populations from Iran and Argentina is described for the first time. Results were compared to previously investigated populations from Israel, Uruguay and Australia. Populations from Iran exhibited high clonality and low gene diversity, suggesting an inoculation event. Populations from uninoculated fields in Argentina had gene and genotype diversities similar to previously described European and North American populations. Genotype diversity was high for populations from Australia and tests for multilocus associations were consistent with sexual recombination in these populations. Gene diversity was low and fixed alleles were found for several loci. These findings are consistent with a relatively small founding population for Australia. These 10 new populations were integrated into a genetic distance comparison with 13 global populations that were characterized earlier. 相似文献
19.
6种杀虫剂对Q型烟粉虱的田间防治效果及抗性测定 总被引:1,自引:0,他引:1
本文测试了6种常用杀虫剂对黄瓜上Q型烟粉虱的田间防治效果,并进行室内毒力测定,以确定Q型烟粉虱对不同药剂的抗性。结果表明,施药后第3天,阿维菌素防治效果达78.8%,其次为苦参&;#8226;内酯,防效达65.1%,其他药剂防效低于45%;施药7d后,6种供试杀虫剂防效均下降到45%以下,且药剂间的差异不显著。毒力测定显示,Q型烟粉虱对6种常用杀虫剂均产生不同程度的抗性,且抗性水平高于B型。其中,Q型对高效氯氰菊酯抗性(抗性倍数47.69)显著高于B型(抗性倍数7.19);对阿维菌素抗性则相对较低,抗性指数仅为4.40;对苦参碱、啶虫脒和吡虫啉的抗性居中(抗性倍数4.57~9.44)。 相似文献
20.
Jose Andres Guevara-Coto Natalia Barboza-Vargas Eduardo Hernandez-Jimenez Rosemarie W. Hammond Pilar Ramirez-Fonseca 《European journal of plant pathology / European Foundation for Plant Pathology》2011,131(2):167-170
Whiteflies are an insect group that comprises multiple species and biotypes, capable of affecting crops by phloem feeding,
virus transmission and promotion of fungal colonization. The distribution of these pests is worldwide. In Costa Rica, a country
located in the tropics, the most problematic whiteflies are Bemisia tabaci biotype B and Trialeurodes vaporariorum. In September 2009, two greenhouses in the Alfaro Ruiz region, northwest of the country’s capital, San Jose, were surveyed
as part of a larger effort to determine the occurrence of species and races of whiteflies in this agronomically important
region. In addition, the insect samples were analyzed to determine the presence of Tomato chlorosis virus (ToCV), a yield-affecting crinivirus transmitted by whiteflies. The results revealed the presence of the Q biotype of B. tabaci, and important invasive species, as well as the expected T. vaporariorum. Viral detection assays identified potentially viruliferous individuals for Tomato chlorosis virus. These results identified a new pest capable of harbouring plant viruses has been identified, as well as a viral agent (ToCV)
in a region where it was not reported, and which might cause significant yield losses. 相似文献