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1.
Two experiments were conducted in the Rift Valley, Ethiopia (8°N and 39°E) to determine associations between eight plant traits
and seed yield, and to obtain estimates of narrow sense heritability for the traits. Experiment I evaluated seven dry edible
bean cultivars/lines at two locations to simulate different soil moisture stress, including, Debre Zeit(non-stress) and Dera
(moderate-stress). Experiment II evaluated 25 cultivars/lines in three environments including, Melkassa early planted (non-stress),
Melkassa late planted (high-stress), and Dera (moderate-stress). A randomized-complete-block design with three replicates
was used in both experiments. Plant traits evaluated were seed yield, pods plant-1, seeds pod-1, 100 seed weight, root dry weight, hypocotyl diameter, plant biomass, plant height and days to flowering. Plant traits that
were significantly associated with seed yield were included in a stepwise-regression model to determine which trait or combination
of traits provided the best model to estimate seed yield in each environment. An analysis of variance was conducted to test
main effects and interactions between plant traits and environments. Significant variation among lines occurred for seed yield
and all plant traits in both experiments. Strong positive correlations were observed between plant biomass and seed yield
in all environments. Seed yield and pods plant-1 were also highly associated in four of the five environments. Stepwise regression models indicated that the combination of
pods plant-1 and plant biomass consistently contributed to seed yield prediction, while other traits did not. Because both plant biomass
and pods plant-1 had moderate to high narrow sense heritability estimates and low GE interactions, they should be useful as indirect selection
criteria to improve and stabilize seed yield in a breeding program.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
2.
M. Santalla M. Lema A. P. Rodiño A. M. González A. B. Monteagudo A. M. De Ron 《Euphytica》2005,142(1-2):85-95
Approaches are needed to broaden the genetic base and improve earliness and yield potential of large-seeded beans under sustainable cropping systems. The objective of this research was to develop adapted dwarf bean populations having a commercial seed quality and yield suitable for the production in the South of Europe. The original base populations were produced from crosses between genotypes within each Mesoamerica, Nueva Granada and Peru races, and between Peru and Nueva Granada, and Mesoamerica and Nueva Granada races. Visual mass selection for plant performance was practised in the F2 and F3 generations. In the F4 and F5, single plants were harvested under two cropping systems (sole cropping and intercropping with maize). From F4, selection was based on precocity, combined with seed yield and seed commercial type. The F4:7 selected lines from each original population were compared with their parents and five checks at four environments and two cropping systems. Differences among environments, populations, parents and checks were observed for all traits. Under intercropping with maize, there was a 50% reduction in seed yield. Yield of parents and checks belonging to Andean South American races, intraracial (Nueva Granada × Nueva Granada) and interracial (Nueva Granada × Peru) populations, was higher than that of those of Middle American origin. Intraracial crosses within large-seeded Andean South American (Peru race) and Middle American gene pools (Mesoamerica race) did not produce lines yielding more than the highest yielding parent. Only two large-seeded lines selected from crosses between small- and large-seeded gene pools out-yielded the best parent and check cultivar. 相似文献
3.
Hercules Renato Corte Magno Antonio Patto Ramalhol Flávia Maria Avelar Gonçalves Ângela de Fátima Barbosa Abreu 《Euphytica》2002,123(3):387-393
Three dry bean harvests are possible in some regions of Brazil that differ significantly in temperature, rainfall and day length conditions. Thus in breeding programs, generation advance using the bulk method can be carried out in these three periods. It is questionable whether under conditions such as these the action of natural selection would contribute to an increased frequency of individuals considered superior by breeders. The present study assessed six segregating populations, for up to 17 successive generations to check the effects of natural selection during inbreeding. Six populations were generated from a partial diallel: one group consisting of two early maturity cultivars, ESAL 686 and Manteigão Fosco was crossed to a second group of three cultivars with medium maturity, Carioca MG, Milionario and Ouro. The six populations plus the five parents were assessed in experiments using a randomized complete block design, in three locations: Lavras, where in breeding started from the F2 generation; Lambari and Patos de Minas, from the F3 generation. A linear regression equation was fitted to the parents and hybrids mean data in each location, considering grain yield as the dependent variable (y) and generations (F2 = 1, F3 = 2, etc.) as the independent variable. Genetic progress was estimated from the differences between the linear regression coefficients of the hybrids (bi)and the mean of the b coefficient of their respective parents (bj). The (bi-bj) were positive in all cases, showing that natural selection acted in all the segregating populations and contributed to an average increase in grain yield of 2.5% per generation over the mean of the parents. 相似文献
4.
Yields of large-seeded Andean (A) common bean (Phaseolus vulgaris L.) cultivars of Chile and Nueva Granada races are 40 to 60% lower compared to their Middle American (M) counterparts of small-seeded Mesoamerica and medium-seeded Durango races. Our objective was to use the concept of congruity backcrossing between Andean x Middle American inter-gene pool [AM 11833 = A 483 (A)///// A 686 (M) //// PVA 800A(A)/// ‘Carioca’ (M)// Carioca (M)/ G 19833 (A)] and between races within Andean gene pool [i.e., intra-gene pool Andean, AA 11834 = A483 (A)//// ‘Cardinal’(A) /// ‘Blanco Español’(A) // BlancoEspañol (A) / ‘Taylor’ (A)] to compare selection for seed yield improvement of large-seeded Andean beans. Seven hundred sixty seven F2-derived F3 (F2:3) families were produced for each population. Visual appraisal for total plant performance, combined with seed yield from non-replicated plots was used for selection of 551 families in F2:3, 182 families in F2:4, and 91 families in F2:5 in each population. Eight hundred twenty three F5:6 lines were developed from the 91 F2:5 families in each population. Visual selection, combined with seed yield in non-replicated plots was again used to select 294 lines in F5:6 in each population. Similarly, 44 highest yielding F5:7 lines were selected in AM 11833 and 39 F5:7 lines in AA 11834. Thus, single plant selections were made in the F2 and F5, and plants within each plot were harvested in bulk in F3, F4, F6, and F7. Thirty nine F5:8 lines from AA 11834 and 44 lines from AM 11833, parents, and checks were evaluated at Popayán and Quilichao, Colombia in 1998 and 1999. Selected lines in both populations, on average, out-yielded the mean of their large-seeded Andean parents. Mean yield of the lines selected from AM 11833 was 50% higher than AA 11834 lines. Twelve F5:8 lines out-yielded the highest yielding Andean parents G 19833 and A 483 in AM 11833, whereas only one line yielded significantly higher (p < 0.05) than the highest yielding parent A 483 in AA 11834. However, none of selected lines out-yielded small-seeded Middle American parents used in AM 11833 (A 686 and ‘Carioca’). The mean 100 seed-weight of AA 11834 was 36 g compared to 28 G for AM 11833 F5:8 lines. Selected lines had similar days to maturity as parents in AM 11833, and matured 3 d later in AA11834. Correlation coefficients between yield and 100 seed-weight were negative in both populations. Yield and days to maturity were positively correlated in AA 11834. 相似文献
5.
N. F. Weeden 《Euphytica》1984,33(1):199-208
Summary Allozyme genotypes were determined at 10 loci for 90 cultivars of white seeded snap beans. Within cultivars the loci were homozygous and usually monomorphic, permitting the characterization of most cultivars by a single set of allozymes. A total of 72 allozyme combinations were observed among the cultivars tested, and 52 (58%) of the cultivars could be uniquely distinguished by allozyme genotype alone. The remaining 38 lines could be separated into small groups of 2–5 cultivars each.This paper has been approved by the Director of the New York State Agricultural Experiment Station as Journal Paper No. 3511. 相似文献
6.
Gamete selection for improving physiological resistance to white mold in common bean 总被引:1,自引:1,他引:0
White mold (WM), caused by Sclerotinia sclerotiorum (Lib.) de Bary, is a widespread disease of dry and green bean (Phaseolus vulgaris L.) in North America. Gamete selection (GS) was effective to combine and pyramide resistant genes and quantitative trait
loci (QTL) for common bacterial blight. Our objective was to determine the effectiveness of GS to introgress physiological
resistance to white mold. Two inter-gene-pool double-cross populations were developed. Selection for WM resistance was practiced
from F1 to F4. Thirteen selected F1:5 breeding lines of each population and their four parents were evaluated. Two separate inoculations were made on each plant
1 week apart using a cut-stem method. The WM reaction was scored at 16, 23, and 33 days post inoculation (DPI) using a scale
from 1 (no disease) to 9 (severely diseased or dead). In F1, 52% of Pop I (USPT-WM-1/CORN 601//USPT-CBB-1/92BG-7) and 67% of Pop II (Chase/I9365-25//ABL 15/A 195) susceptible plants
were discarded. In F4, only 1.2% of families from Pop I, and 0.9% for Pop II, survived the selection process. An average of 20.5% gain in WM resistance
was obtained for both populations in F4. Four breeding lines of Pop I had significantly (P = 0.05) lower WM score (4.1–4.6) and four were equal (4.7–4.9) to the best WM-resistant parent 92BG-7 (4.9), while ten breeding
lines of Pop II were equal (4.5–4.8) to the best WM-resistant parent A 195 (4.6). Thus, GS was effective for improving WM
resistance in common bean. 相似文献
7.
Summary The bean weevil Acanthoscelides obtectus, is an important storage pest of common beans in Latin America and Africa. A few wild bean accessions from Mexico have been identified as highly resistant to the weevil. One accession, G 12952, was crossed to two susceptible bean cultivars differing in seed size. Reciprocal F1 and F2 individual seed were evaluated for days to adult emergence (DAE) and emerged adult weight. Maternally inherited seed size affected resistance measurements only in the F1 reciprocal crosses, however, the overall resistance level of the F1 was more similar to that of the susceptible cultivars. The F2 showed a continuous, but skewed distribution from low to high DAE. Very few F2 individuals had the resistance level of G 12952. When the frequency distributions were divided into discrete categories based on parental response, resistance was found to be inherited as two recessive complementary genes. The F3 generation showed an overall lowering of resistance levels compared to their original F2 evaluations. However, none of the lines classified as resistant (50 DAE) in the F2, fell into the susceptible category in the F3, indicating that the resistant genotypes were relatively stable as expected with recessively inherited traits. Modifying genes from the commercial parents may be responsible for general lowering of resistance. Seed size was negatively correlated with adult weight but not with DAE. The unique resistance of the wild bean accessions is discussed in relation to its inheritance. The results and obstacles encountered in the A. obtectus breeding program at CIAT are described. 相似文献
8.
Anju Pathania P. N. Sharma O. P. Sharma R. K. Chahota Bilal Ahmad P. Sharma 《Euphytica》2006,149(1-2):97-103
Summary Forty nine common bean lines comprising of exotic accessions and locally grown cultivars evaluated against Colletotrichum lindemuthianum exhibited differential resistance to its races in Himachal Pradesh, a north-western Himalayan state of India. Some exotic accessions like G 2333, Cornell 49242, PI 207262, Mexique 222, TO, Perry Marrow, Kaboon and Widusa were resistant to more than five Indian races, whereas two Indian accessions KRC-5 and Hans showed resistance to six and four races, respectively. However, nine accessions KRC-8, KR-40, KR-43, KR-81, KR-62-2, KR-90, KR-142, KR-148, and KR-216 were resistant to three races. Race specific resistance has been observed in different bean cultivars. Studies on inheritance of resistance in exotic accession G 2333 and Indian accession, KRC-5 showed that two independent dominant genes conferred resistance in G 2333 to race 3 and 515 and a single dominant gene controlled resistance in KRC-5 to race 775, indicating resistance from these sources is easily transferable to the locally adapted susceptible cultivars. 相似文献
9.
To evaluate the variability among cultivars and landraces of common bean(Phaseolus vulgaris L.), 15 cultivars and 18 landraces of common bean (Phaseolus vulgarisL.), a undefined species of Phaseolus,two landraces of Vigna angularis L., and a landrace of soybean (Glycine maxL.), were screened with fifteen oligonucleotide primers in PCR reactions. An average of 20.3 RAPD bands were scored per primer.
A total of 304 amplification products were scored of which 88.8% were polymorphic among Phaseolus genotypes. Based on the RAPD markers, four major clusters were formed. Three clusters corresponded to the soybean, to the
two Vigna angularis landraces, and to the Phaseolus sp. landrace, respectively. The fourth cluster include all the landraces and cultivars of Phaseolus vulgaris. This large group could be separated into three subgroups that were correlated with the phaseolin patterns and the average
seed weight of the genotypes. The analysis shows that most of the landraces collected in South Brazil (17 out of 18) belong
to the Andean gene pool, and most of the cultivars (13 out of 15) belong to the Middle American gene pool.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
10.
Dry bean (Phaseolus vulgaris L.) is an important grain legume for small-scale farmers in eastern Africa who nonetheless, grow beans with limited phosphorus
(P) fertilizer supply or none at all. Phosphorus rank second, after nitrogen (N), as the most limiting soil nutrient in bean
production in East African soils. This study was conducted to determine combining ability for five polygenic traits in the
red mottled, large seeded bean market class, under low and high soil P conditions and two locations. Three parents tolerant
to low soil P were hybridized with five well adapted, but non-low P tolerant lines in a diallel mating scheme. The resulting
28 F1 hybrids were evaluated in a randomized complete block design with three replications, under low and high soil P conditions
at two sites. There were highly significant (P ≤ 0.001) differences among the genotypes for all the traits under all the study conditions. The GCA mean squares were highly
significant (P ≤ 0.001) for these traits, indicating importance of additive effects for both study conditions and sites. The GCA × Environment
and SCA × Environment were significant for all the parameters and test conditions. CAL143 had positive GCA effects that were
significant; except for 100-seed weight under P stress; for all the traits and under all the study conditions. The negative
GCA effects for the none P tolerant parents indicate that they impacted positively in imparting earliness. 相似文献
11.
Magno Antonio Patto Ramalho Ângela de Fátima Barbosa Abreu João Bosco dos Santos 《Euphytica》2005,144(1-2):23-29
The objective of this study was to evaluate the genetic progress after four cycles of recurrent selection in common bean.
The base segregating population was obtained from 10 parents, and derived the S0:1 and S0:2 families that were evaluated. The S0:3 families with higher grain yield and grain color, like the standard carioca were selected, and were intercrossed to generate
the population of the following cycle. This process was repeated for four cycles. The best families were evaluated in each
cycle by many generations and locations, and the five best lines of each cycle were identified. The 20 lines thus obtained
were evaluated in two growing seasons, sown in July and November 2002. The grain yield (kg/ha) and grain type (scale of scores)
were evaluated. Genetic progress was confirmed for both traits. The mean annual gain with selection for the grain type was
10.5% and 5.7% for grain yield, with no evidence of variability reduction in the population. These results show that recurrent
selection is a good alternative for improving common bean quantitative traits. 相似文献
12.
Summary The relationship between the yield potential of crosses for several generations and the yield of either the parental varieties or early segregating generations was studied. It was found that if plants were grown at crop densities then a high degree of prediction of cross potential was possible independent of year or generation. Prediction was much less efficient however if either the parents or the early generations were grown as spaced plants. It is concluded that at crop densities an accurate assessment of parental yields is sufficient to determine which crosses are most likely to have a high yield potential and that if any cross proves to be low yielding in early generations it may safely be rejected without fear of losing potentially high yielding crosses. Reasons for the failure of predictions, as reported in the literature, are discussed. 相似文献
13.
Marta Santalla M. Carmen Menéndez-Sevillano Ana B. Monteagudo Antonio M. De Ron 《Euphytica》2004,135(1):75-87
Wild populations of common bean pertainingto the Andean gene pool are distributedfrom southern Peru to northern Argentina.The objectives of this study were todetermine the genetic structure of Andeanlandraces from northern Argentina, as oneof the potential domestication sites of theAndean domesticated gene pool, and toestablish a correspondence between Andeanprimitive landraces and wild populationsthat might have served as the source ofdomesticated bean. Forty-four landraces and21 wild populations representing thediversity of common bean in northernArgentina were included in this study. Results indicated that Andean gene pool inArgentina has a large genetic base on thebasis of morphological and adaptivevariability and biochemical analysis. Theexistence of introgressed populations withsympatric wild forms was evidenced. 相似文献
14.
In pearl millet (Pennisetum glaucum (L.) R. Br.), single cross hybrids based largely on exotic germplasm, have found very limited adoption in drought-prone arid
regions mainly due to their inadequate adaptation to prevalent extremely harsh agro-climatic conditions. This study tested
the hypothesis that the use of restorer parents derived from adapted landrace germplasm could be an alternative strategy in
developing suitable hybrids for arid regions. Forty inbred restorer lines developed from two phenotypically diverse landraces
were used to develop hybrids evaluated in this study for their performance in five typical arid zone environments between
2003 and 2007. Inbred lines from both landrace populations differed significantly in their combining ability for biomass,
harvest index and grain and stover yields. A larger proportion of experimental hybrids, based on selected inbred lines, significantly
outperformed the commercial checks for grain and stover yields. Increase in grain and stover yields was achieved primarily
due to their higher biomass productivity, with no decline in harvest index. Choice of landrace determined the relative grain
and stover productivity of their hybrids. Phenotypic differences observed in parental landraces in tillering and panicle length
were also visible in their respective hybrids, indicating that characteristics of landrace parental populations were transmitted
in their hybrids. 相似文献
15.
Summary A simple protocol for leaf rooting in beans (Phaseolus vulgaris L.) was developed and used to investigate the reaction of Xanthomonas campestris pv. phaseoli (Xcp), causal pathogen of common bacterial blight disease, in detached versus attached bean leaves. Trifoliate leaves of different sizes (one-third, two-thirds, and fully expanded), either with or without the pulvinus attached to the petioles, were excised from 20 day-old plants of six bean cultivars/lines. Leaf cuttings were cultured in potting medium and then incubated for 5 to 10 days under transparent polyethylene plastic cover in the greenhouse. Roots were readily initiated along the petioles of the leaf cuttings, whether the pulvinus was present or absent. All leaves which were two-thirds expanded and fully expanded developed roots 5 to 7 days after culture. Eighty to 90 percent of the leaves which were one-third expanded formed roots 8 to 10 days after incubation. Laminae of the rooted leaf cuttings were viable and green during the 2 to 3 months period in culture after removing the plastic cover. The common bacterial blight reactions were similar for inoculated attached leaves, detached rooted leaves (inoculated either after or prior to rooting), and moistened detached leaves incubated without rooting. The latter were only usable for evaluation of the Xcp reaction in growth chamber experiments but not under greenhouse conditions. The rooted leaves would be useful for screening bean lines for multiple disease resistance, especially if the pathogens require different environments for disease expression.Abbreviations CBB
Common Bacterial Blight
-
Xcp
Xanthomonas campestris pv. phaseoli (Smith) Dye 相似文献
16.
Ana Lilia Alzate-Marin Marcia Regina Costa Klever Marcio Arruda Everaldo Gonçalves de Barros Maurilio Alves Moreira 《Euphytica》2003,133(2):165-169
Ouro Negro (Honduras 35) is a highly productive Mesoamerican black seeded bean cultivar that possesses a major dominant gene
conferring resistance to anthracnose (causal organism Colletotrichum lindemuthianum). In this work the anthracnose resistance gene present in Ouro Negro was characterized by studying allelic relationships
to the following previously characterized anthracnose resistance genes (cultivars): Co-1 (MDRK), Co-1
2 (Kaboon), Co-1
3 (Perry Marrow), Co-2 (Cornell 49-242), Co-3 (Mexico 222), Co-4 (TO), Co-4
2 (SEL 1308), Co-5 (SEL1360), Co-6 (AB 136), and the resistance genes present in PI 207262 and Widusa. In addition, we determined the resistance spectrum of
Ouro Negro in relation to 19 pathotypes of C. lindemuthianum. The allelism tests confirmed that the dominant anthracnose resistance gene present in Ouro Negro is positioned at a locus
distinct from those with which it was compared. We propose that this new gene be named Co-10. The inoculation of Ouro Negro with the 19 pathotypes of C. lindemuthianum demonstrated that Co-10 confers resistance to pathotypes 23, 64, 67, 73, 81, 83, 87, 89, 95, 102, 117, 119, 343, 453, 1033, 1545 and 1600. The identification
of Co-10 is an important contribution to bean breeding programs that are in constant need of new sources of resistance to anthracnose.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
17.
Molecular markers and allelic relationships of anthracnose resistance gene cluster B4 in common bean
Angular leaf spot is one of the major diseases of the common bean. The extensive genetic variability of this pathogen requires
the constant development of new resistant cultivars. Different sources of resistance have been identified and characterized.
For the State of Minas Gerais, Brazil, four main resistance sources were found: Mexico 54, AND 277, MAR 2 and Cornell 49-242.
Independent characterization of these genotypes demonstrates that resistance in all four sources is dominant and monogenic.
However, there are no studies on the relationship and independence of these genes. In the present work, allelism tests were
carried out to understand the relationship among the resistance genes present in these four resistance sources. The data revealed
a much higher complexity in the resistance inheritance of these genes than previously reported. It was demonstrated that Cornell
49-242 possesses a dominant gene (Phg-3); Mexico 54 possesses three genes, denominated Phg-2, Phg-5 and Phg-6. In MAR 2, two genes were found, one independent designated Phg-4 and the other, an allelic form of Phg-5, denominated of Phg-52. Allelic forms were also found in AND 277, Phg-22, Phg-32 and Phg-42. These results have special importance for breeding programs aiming to pyramid resistance genes. 相似文献
18.
Summary A method to inoculate detached bean leaves with Botrytis cinerea or Sclerotinia sclerotiorum without using a carbon-nitrogen source is described. Leaves were incubated in plastic trays that were covered with transparent plastic bags to maintain air humidity above 95%. Spores of either fungus inoculated in water alone were generally unable to cause infection. Additions of KH2PO4 (62.5 mM) or of mixtures of inorganic phosphate and glucose (1.1 or 5.5 mM) to the inoculum stimulated fungal pathogenicity. Also spore concentration (2×105 or 2× 106 spores ml–1) influenced the ability to produce lesions. At 2× 106 spores ml–1 both fungi were able to infect primary bean leaves when inoculated in an inorganic phosphate solution. These conditions were used for a preliminary screening for resistance of 15 bean cultivars to both fungi. Only partial resistance to the pathogens was found. Since the procedure is simple and does not require blooming plants, it can be rapidly adopted in screening and breeding programs.Abbreviations Pi
inorganic phosphate 相似文献
19.
Marília Caixeta Franco Sérvio Tulio Cassini Valter Rodrigues Oliveira Clibas Vieira Siu Mui Tsai Cosme Damiao Cruz 《Euphytica》2001,118(3):265-270
Ten F1's obtained from crosses among five common bean genotypes of Andean (WAF 15, Mineiro Precoce and Batatinha) and Middle American
(BAT 304 and Ouro) gene pools were assessed for their combining abilities for root nodulation with Rhizobium tropici strain CIAT 899. The plants were grown under controlled conditions and evaluated for number of nodules per plant (NN), nodule
dry weight (NDW), mean nodule weight (MNW) and plant fresh weight (PFW). The subdivision of the treatment effects on the general
(GCA) and specific combining effects (SCA) were performed according to Griffing's diallel analysis method 2, model 1. The
analyses of variance and estimates of quadratic components showed that non-additive gene effects were more important in the
expression of NN and PFW, whereas additive gene effect was predominant for NDW and MNW. A close association was observed between
high number of nodules and GCA. Generally, crosses involving parents of different gene pools yielded hybrids with high positive
estimates of SCA for all assessed traits. The genotypes of Andean origin WAF 15 and Mineiro Precoce are the most promising
parents for breeding programs to increase NN and NDW in common beans.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
20.
Paolo Ranalli 《Euphytica》1996,87(2):127-132
Summary The effect of a recurrent selection procedure for improving seed yield per plant was evaluated in common bean. Progress was registered after three cycles of recurrent selection with S2 progeny evaluation performed on the grain yield/plant. Gain from selection was estimated by field testing 45 random S2 lines from each of the C0, C1 and C2 populations. Response to selection for seed yield/plant was 6 g from C0 to C1 and 4.4 g from C1 to C2. The seed yield improvement was due principally to an increase in pods/plant; little contribution was provided by the 1000-seed weight and seeds/pod traits. Broad-sense heritability and genotypic variance for grain yield remained high in all cycles which suggests further gain from additional selection cycles. Grain yield/plant is significantly correlated to pods/plant and seeds/pod traits in all cycles, indicating that plants with favourable combinations of these traits could be identified. The proposed recurrent selection procedure appears to be effective in improving the population and extracting superior genotypes for varietal development. 相似文献