Agronomic and qualitative traits of T. turgidum ssp. dicoccum genotypes cultivated in Italy

Summary The increasing popularity of organic agriculture and health food products has led to a renewed interest in hulled wheat species such as emmer (Triticum turgidum ssp. dicoccumSchubler). Knowledge on agronomic and quality traits is required for effective and efficient use of germplasm collections in breeding programs. The objective of this study was to estimate agronomic and grain quality traits of emmer wheat cultivated in Italy. A total of 20 emmer accessions consisting of landraces, breeding lines or cultivars selected from landraces and modern cultivars were examined under low input conditions. The study was conducted for three successive years (2002–2004) at one location of Southern Italy (Foggia). The entries were characterized for agronomic and qualitative traits [grain yield (t ha⁻¹), thousand grain weight (g), test weight (kg hl⁻¹), grain protein content (%), HMWG composition, dry gluten content (%), gluten index and yellow index, alveograph indices and Total Organic Matter (TOM) on cooked pasta]. The results showed a large genetic variability for most of the traits measured and, even if most of the accessions showed inferior bread- and pasta-making performance, modern cultivars exhibited improved quality traits with some potential to perform healthy and tasty food.     

Molecular assessment of genetic diversity in winter barley and its use in breeding

Summary During the last decades extensive progress has been achieved in winter barley breeding with respect to both, yield and resistance to fungal and viral diseases. This progress is mainly due to the efficient use of the genetic diversity present within high yielding adapted cultivars and – with respect to resistance – to the extensive evaluation of genetic resources followed by genetic analyses and introgression of respective genes by sexual recombination. Detailed knowledge on genetic diversity present on the molecular level regarding specific traits as well as on the whole genome level may enhance barley breeding today by facilitating efficient selection of parental lines and marker assisted selection procedures. In the present paper the state of the art with respect to virus diseases, i.e. Barley mild mosaic virus, Barley yellow mosaic virus, and Barley yellow dwarf virus is briefly reviewed and first results on a project aiming on a genome wide estimation of genetic diversity which in combination with data on yield and additional agronomic traits may facilitate the detection of marker trait associations and a more efficient selection of parental genotypes are presented. By field tests of 49 two-rowed and 64 six-rowed winter barley cultivars the genetic gain in yield for the period 1970–2003 was estimated at 54.6 kg ha⁻¹ year⁻¹ (r² = 0.567) for the six-rowed cultivars and at 37.5 kg ha⁻¹ year⁻¹ (r² = 0.621) for the two-rowed cultivars. Analysis of 30 SSRs revealed a non-homogenous allele distribution between two and six-rowed cultivars and changes of allele frequencies in relation to the time of release. By PCoA a separation between two and six-rowed cultivars was observed but no clear cut differentiation in relation to the time of release. In the two-rowed cultivars an increase in genetic diversity (DI) from older to newly released cultivars was detected.     

Solar radiation interception and canopy expansion of sweet corn in response to phosphorus

Insufficient phosphorus (P) availability decreases the yield of Zea mays, particularly for sweet corn crops grown in cool environments. This research examined the mechanisms of yield reductions with initial emphasis on canopy expansion processes that affect the interception of solar radiation. Experiments in two consecutive seasons (2001/2002 and 2002/2003) were grown at a low P site (Olsen P = 6 μg ml⁻¹) at Lincoln, New Zealand. Each experiment contained five rates of P application. In 2001/2002 rates of 0, 50, 100, 150, or 200 kg P ha⁻¹ were applied. In 2002/2003 an additional 0, 0, 10, 20 or 40 kg P ha⁻¹ was applied to the same plots producing total P treatments of 0, 50, 110, 170 or 240 kg P ha⁻¹ summed over the two seasons.When P availability was limited (0 or 50 kg P ha⁻¹) the rates of leaf tip and fully expanded leaf appearance were slower in both seasons. Phyllochrons (°Cd leaf tip⁻¹) were 5 °Cd longer in crops that received 0 kg P ha⁻¹ than those fertilised with ≥100 kg P ha⁻¹. The area of individual leaves was also reduced by low P inputs but the ranking of leaf area by main stem leaf position was conservative. The leaf area of the largest leaf of the unfertilised crops was at least 22% less than the maximum measured leaf area in both seasons. In contrast, P fertiliser application had no effect on leaf senescence.The rate of leaf appearance per plant, individual leaf area and plant population were integrated to calculate green leaf area index (GLAI) and to estimate accumulated radiation interception (RI_cum) for these crops. The total RI_cum throughout the season in the unfertilised crops was 12–28% less than for those crops that received ≥100 kg P ha⁻¹ in both seasons. This difference partly explained the differences in crop biomass production in response to P availability. A sensitivity analysis showed that RI_cum was equally sensitive to changes of the rate of leaf appearance and the area of individual leaves in response to P supply. Both processes need to be incorporated in mechanistic models of P effects on Z. mays which can be used to design efficient P fertiliser strategies.     

Forage production,quality and water-use-efficiency of four warm-season annual crops at three sowing times in the Loess Plateau region of China

Increasing demand for livestock products is driving development of livestock systems worldwide. That requires improved and new forage production options. The Loess Plateau region in central-northern China is an important area for livestock production, as it supports11% and 19% of the country’s cattle and sheep, respectively (China statistical yearbook 2014). The rain-fed semi-arid environment of the Loess Plateau means that maximizing the water-use-efficiency (WUE) of forage production is vital to guarantee enough fodder supply the livestock demand. A three-year field experiment in north-west Loess Plateau compared forage production, water use and water-use-efficiency as well as crude protein (CP) content of forage maize, Sudan grass, foxtail millet and Japanese millet sown at three sowing dates according to the opening rain during 2011–2013. On average, forage maize produced the highest biomass (12.1 t ha⁻¹) and had the highest WUE (43.4 kg DM ha⁻¹ mm⁻¹). This was followed by Sudan grass (7.8 t ha⁻¹; 26.5 kg DM ha⁻¹ mm⁻¹), Japanese millet (6.7 t ha⁻¹; 26.2 kg DM ha⁻¹ mm⁻¹) and foxtail millet (6.7 t ha⁻¹; 24.6 kg DM ha⁻¹ mm⁻¹). Optimizing sowing date played an important role in maximizing forage production and WUE of all tested forages. Compared to the earliest sowing date, a delay of two weeks reduced forage production by 17% in maize, 35% in foxtail millet, and 16% in Japanese millet. A delay of four to six weeks reduced biomass yield by 58% in maize, 57% in foxtail millet, and 56% in Japanese millet. Late sowing also greatly reduced WUE of forage maize and foxtail millet by 33% and 42%, respectively, when compared to early sowing. The middle sowing date maximized forage production and WUE of Sudan grass in two of the three growing seasons, which was 20% and 38% higher than the early and late sowing, respectively. Late sowing in all forages reduced crop water use by 42–57 mm compared to the early sowing. Among four test crops, CP of Sudan grass (7.9%) and forage maize (7.7%) was higher than foxtail millet (6.8%) and Japanese millet (6.7%). Compared with early sowing, CP_f in late sowing significantly increased in Sudan grass and decreased in Japanese millet, in contrast, no evident sowing date effect was found in forage maize and foxtail millet. This study showed that all four warm-season annual grasses had high forage production potential, forage maize was the most reliable and efficient option. Forage maize and the millets could easily be integrated into existing cropping systems and provide opportunities as both grain and forage-producing crop to provide added flexibility for farmers.     

Performance of various grapefruit (<Emphasis Type="Italic">Citrus paradisi</Emphasis> Macf.) and pummelo (<Emphasis Type="Italic">C. maxima</Emphasis> Merr.) cultivars under the dry tropic conditions of Mexico

Grapefruit growers in the tropics require information about existing and new citrus cultivars with high productivity potential. The objective of this study was to determine the growth, yield, and fruit quality performance of seven pigmented and four white grapefruit cultivars under the dry tropic conditions of Colima, Mexico. The trees were budded on sour orange (Citrus aurantium L.) rootstock and planted at a distance of 8 × 4 m. ‘Oroblanco’ and ‘Marsh Gardner’ white-fleshed grapefruit cultivars and ‘Chandler’, a pink-fleshed pummelo, were the largest trees with the greatest height (5.0–5.6 m), canopy diameter (6.2–6.3 m), trunk diameter (21.9–23.3 cm), and canopy volume (109–123 m³). Lower height (4.3–4.8 m) and canopy volume (73–96 m³), but with similar canopy diameter to the previously mentioned cultivars, were recorded for the remaining pigmented cultivars. ‘Chandler’ pummelo and four pigmented grapefruit cultivars (‘Shambar’, ‘Río Red’, ‘Ray Ruby’, and ‘Redblush #3’) had yearly productions of 34.8, 34.9, 34.1, 32.7, and 30.6 ton ha⁻¹, respectively. The most productive white grapefruit cultivar was ‘Marsh Gardner’ (30.5 ton ha⁻¹). Grapefruit cultivars having the largest fruit size showed a higher inverse relationship between fruit weight and yield than those with small fruit. Most genotypes had higher values of fruit weight, juice content, and maturity index than those required by the local market. The most promising grapefruit cultivars based on their acceptable growth, yield superior to 30 ton ha⁻¹, and acceptable fruit color were ‘Río Red’, ‘Shambar’, ‘Ray Ruby’, and ‘Redblush #3’.     

Nutritional and physicochemical variation in Turkish kabuli chickpea (<Emphasis Type="Italic">Cicer arietinum</Emphasis> L.) landraces

We examined the physical, physicochemical, and cooking properties of 91 kabuli chickpea landraces from Turkey, using two cultivars (İnci and İzmir-92) as controls. All landraces were grown under the same agroclimatic conditions to eliminate variance caused by genotype × environment interactions. There was high diversity among the landraces in the contents of protein (17.55–23.31%), fat (4.45–6.11%), ash (2.54–3.41%), fiber (2.03–4.18%), starch (41.76–49.07%), moisture (6.39–10.57%), 100-seed weight (25.03–51.67 g), hydration capacity (0.2585–0.6169 g/ml), swelling index (0.7207–1.1859), swelling capacity (0.15–0.32 ml seed⁻¹), cooking time (33–72 min), and seed density (0.8450–1.4800 g/ml). Regional analysis of landraces showed significant differences with respect to nutritional characteristics and cooking parameters. There were also significant relationships between various seed quality and cooking parameters. Together, our results provide an initial step toward identification of chickpea landraces that may be useful for development of high-quality chickpea cultivars.     

Selection of wheat based on economic returns per unit area

This study aimed to investigate a cultivar selection criterion based on income ha⁻¹ in common wheat. Regional yield trials with 20 entries were planted in Diyarbakır, Hazro and Ceylanpınar in southeast Anatolia in the 2004/2005 growing season. A randomized complete block design with four replications was employed. Grain samples from each location were subjected to quality analyses, and then presented to randomly selected grain purchasers with the local commodity market for market price estimations. Entries 1, 9, 10, 7 and 6 were the top five ranking entries for grain yield, giving 5,320, 5,290, 5,280, 5,140 and 5,130 kg ha⁻¹, respectively. From the market price perspective, entries 17, 3, 7, 20 and 8 received the five highest marketing price offers with the values 239.0, 238.1, 237.1, 236.9 and 236.7 US$ tonne⁻¹, respectively. There was a US$ 10.94 tonne⁻¹ market price difference between entries with the highest and the lowest market price. The only quality analyses showing significant correlations with market price were Zeleny sedimentation value and hectolitre weights (kg hl⁻¹). From thev production income [= marketing price (US$ tonne⁻¹) × grain yield (kg ha⁻¹)] point of view, entries 9, 10, 1, 7 and 6 were ranked from 1st to 5th for production income ha⁻¹ with the values 1,241.0, 1,238.0, 1,219.9, 1,214.2 and 1,209.3 US$ ha⁻¹, respectively. All five high yielding entries were also high-income entries. A rank stability analysis further indicated that entry numbers 6, 7, 8, 4 and 1 stable for high production income ha⁻¹. A simulation study, based on allocation of additional premiums for high quality indicated that entries 9,1,10, 7, and 6 would be top ranking for high income ha⁻¹ if given an additional premium of as much as twice the standard deviations of market price for each entry (the highest premium limit; 20.24 US$ tonne⁻¹). The order for cultivar preference for high production income ha⁻¹ would change and would generate 1,321.94, 1,296.20, 1,281.11, 1,258.06 and 1,243.01 US$ ha⁻¹, respectively. It was concluded that the relatively quality conscious Şanlıurfa commodity market does not offer adequate premiums for the high quality grains. This results in farmer preference for high yielding lower quality cultivars. Even though quality cannot be neglected, and cultivar preference could change with additional premiums, breeders must give more attention to high production income ha⁻¹.     

西北绿洲灌区水氮耦合对燕麦品种陇燕3号耗水特性及产量的影响

灌水和施肥,尤其是施氮肥,是调控作物生长和增加产量的两大重要技术措施,其互作是燕麦高产高效栽培中重要因素。2014—2015年连续2个生长季,在甘肃河西绿洲灌区的田间试验设3个定额灌溉和3个施氮(纯氮)水平,研究水氮耦合对陇燕3号农田0~150 cm土层耗水量、棵间蒸发、产量及水分利用效率的影响。3个灌溉处理的灌水量分别为270 mm(I_1)、337.5 mm(I_2)和405 mm(I_3),3个施氮水平分别为90 kg hm~(–2)(N_1)、120 kg hm~(–2)(N_2)和150 kg hm~(–2)(N_3)。从播种到成熟,燕麦阶段耗水强度呈先增后减趋势,抽穗至灌浆是最大耗水期,且同一施氮水平下,阶段耗水强度随灌水量增大而显著增加。在全生育期内,棵间蒸发量(E)及土壤水分蒸发量占总蒸发量的比例(E/ET)表现先降后升趋势,且相同施氮量下,拔节至灌浆期随灌水量的增大而增大,而灌浆至成熟期则随灌水量的增大而减小。相同施氮量下,燕麦产量随灌水量增加而显著增加,水分利用效率却随灌水量增加而降低。产量N_3I_3最高(5466.0~5727.5 kg hm~(–2)),N_3I_2次之(5428.5~5678.5 kg hm~(–2)),N_1I_1最低(4504.5~4804.3 kg hm~(–2));水分利用效率N_3I_2最大(12.11~12.82 kg mm~(–1) hm~(–2)),N_3I_1次之(12.04~12.63 kg mm~(–1) hm~(–2)),N_1I_3最小(9.79~10.58 kg mm~(–1) hm~(–2))。由此表明,水氮耦合对燕麦水分利用及产量具有显著互作效应。施氮量150 kg hm~(–2)、灌溉定额337.5 mm是西北绿洲灌区燕麦种植较佳的节水、高产水氮管理模式。     

Maize yield and water balance is affected by nitrogen application in a film-mulching ridge–furrow system in a semiarid region of China

Poor soil and drought stress are common in semiarid areas of China, but maize has a high demand for nitrogen (N) and water. Maize production using the technique of double ridges and furrows mulched with plastic film are being rapidly adopted due to significant increases in yield and water use efficiency (WUE) in these areas. This paper studied N use and water balance of maize crops under double ridges and furrows mulched with plastic-film systems in a semiarid environment over four growing seasons from 2007 to 2010. To improve precipitation storage in the non-growing season, the whole-year plastic-film mulching technique was used. There were six treatments which had 0, 70, 140, 280, 420 or 560 kg N ha⁻¹ applied in every year for maize. In April 2011, spring wheat was planted in flat plots without fertilizer or mulch following four years of maize cultivation. After four years, all treatments not only maintained soil water balance in the 0–200 cm soil layer but soil water content also increased in the 0–160 cm soil layer compared to values before maize sowing in April 2007. However, under similar precipitation and only one season of spring wheat, soil water content in the 0–160 cm soil layer sharply decreased in all treatments compared to values before sowing in April 2011. Over the four years of maize cultivation, average yield in all treatments ranged from 4071 to 6676 kg ha⁻¹ and WUE ranged from 18.2 to 28.2 kg ha⁻¹ mm⁻¹. In 2011, the yield of spring wheat in all treatments ranged from 763 to 1260 kg ha⁻¹ and WUE from 3.5 to 6.5 kg ha⁻¹ mm⁻¹. The potential maximum grain yield for maize was 6784 kg ha⁻¹ with 360 kg N ha⁻¹ applied for four years, but considerable NO₃N accumulated in the soil profile. A lesser application (110 kg N ha⁻¹) to this tillage system yielded in 82% of the maximum, increased nitrogen use efficiency and mitigated the risk of nitrogen loss from the system. This study suggests that double ridge–furrow and whole-year plastic-film mulching could sustain high grain yields in maize with approximately 110 kg N ha⁻¹ and maintain soil water balance when annual precipitation is >273 mm in this semiarid environment.     

Diallel analysis of grain yield and resistance to seven diseases of 12 African maize (<Emphasis Type="Italic">Zea mays</Emphasis> L.) inbred lines

Maize (Zea mays L.) is grown on 15 million ha in eastern and southern Africa. Several diseases are of common occurrence in the region and regularly result in significant yield losses. A collaborative regional disease nursery (REGNUR) project was initiated in 1998 to identify and increase access to disease resistant germplasm, generate and disseminate information on disease and insect resistance sources, and facilitate the development of resistant cultivars by project partners. A diallel among 12 elite inbred lines was formed with the specific objective of evaluating the combining ability of these inbred lines for grain yield and resistance to seven diseases. The trial was grown at six sites in 2001. Results showed that both general (GCA) and specific combining ability effects were significant for most diseases. On the average, GCA accounted for 69% of resistance to diseases and only 37% of variation for grain yield. Correlations between GCA effects for disease scores were generally non-significant, implying that it is possible to pyramid genes for disease resistance in inbred lines. This underscores the need for screening for resistance to prevailing diseases using artificial inoculation or reliable hot-spots. Based on GCA effects for grain yield and across diseases, P12 and P6 were the best inbred lines. The crosses P4 × P9 (6.7 t ha⁻¹) and P4 × P12 (6.9 t ha⁻¹) were the best hybrids in the earlier maturity group, while P3 × P9 (8.3 t ha⁻¹) and P2 × P8 (7.4 t ha⁻¹) were the best hybrids in the late maturity group.     

Explaining rice yields and yield gaps in Central Luzon,Philippines: An application of stochastic frontier analysis and crop modelling

Explaining yield gaps is crucial to understand the main technical constraints faced by farmers to increase land productivity. The objective of this study is to decompose the yield gap into efficiency, resource and technology yield gaps for irrigated lowland rice-based farming systems in Central Luzon, Philippines, and to explain those yield gaps using data related to crop management, biophysical constraints and available technologies.Stochastic frontier analysis was used to quantify and explain the efficiency and resource yield gaps and a crop growth model (ORYZA v3) was used to compute the technology yield gap. We combined these two methodologies into a theoretical framework to explain rice yield gaps in farmers’ fields included in the Central Luzon Loop Survey, an unbalanced panel dataset of about 100 households, collected every four to five years during the period 1966–2012.The mean yield gap estimated for the period 1979–2012 was 3.2 ton ha⁻¹ in the wet season (WS) and 4.8 ton ha⁻¹ in the dry season (DS). An average efficiency yield gap of 1.3 ton ha⁻¹ was estimated and partly explained by untimely application of mineral fertilizers and biotic control factors. The mean resource yield gap was small in both seasons but somewhat larger in the DS (1.3 ton ha⁻¹) than in the WS (1.0 ton ha⁻¹). This can be partly explained by the greater N, P and K use in the highest yielding fields than in lowest yielding fields which was observed in the DS but not in the WS. The technology yield gap was on average less than 1.0 ton ha⁻¹ during the WS prior to 2003 and ca. 1.6 ton ha⁻¹ from 2003 to 2012 while in the DS it has been consistently large with a mean of 2.2 ton ha⁻¹. Varietal shift and sub-optimal application of inputs (e.g. quantity of irrigation water and N) are the most plausible explanations for this yield gap during the WS and DS, respectively.We conclude that the technology yield gap explains nearly half of the difference between potential and actual yields while the efficiency and resource yield gaps explain each a quarter of that difference in the DS. As for the WS, particular attention should be given to the efficiency yield gap which, although decreasing with time, still accounted for nearly 40% of the overall yield gap.     

Optimized single irrigation can achieve high corn yield and water use efficiency in the Corn Belt of Northeast China

Decreasing the corn (Zea mays L.) gap between the potential yield and farm yield and reducing the risk of grain yield of drought are very important for corn production in the Corn Belt of Northeast China (CBNC). To achieve a high and stable corn yield, the effects of supplementary irrigation on yield, water use efficiency (WUE) and irrigation water use efficiency (IWUE) were studied using a modelling approach. The Root Zone Water Quality Model 2 was parameterized and evaluated using two years of experimental data in aeolian sandy soil and black soil. The evaluated model was then used to investigate responses to various irrigation strategies (rainfed, full irrigation and 12 single irrigation scenarios) using long-term weather data from 1980 to 2012. Full irrigation guarantees a high and stable corn grain yield (12.92 Mg ha⁻¹ and has a coefficient of variation (CV) of 14.8% in aeolian sandy soil; 12.30 kg Ma⁻¹ and CV of 11.1% in black soil), but has a low water use efficiency (19.92 and 21.81 kg ha⁻¹ mm⁻¹) and a low irrigation water use efficiency (10.01 and 11.03 kg ha⁻¹ mm⁻¹). A single irrigation can increase corn yields by 3–35% for aeolian sandy soil and 5–35% for black soil over different irrigation dates compared with no irrigation. The most suitable single irrigation date was during late June to early July for aeolian sandy soil (yield = 10.73 Mg ha⁻¹ and WUE = 27.94 kg ha⁻¹ mm⁻¹) and early to mid-July for black soil (yield = 11.20 Mg ha⁻¹ and WUE = 27.70 kg ha⁻¹ mm⁻¹). The lowest yield risk of falling short of the yield goal of 8, 9, and 10 Mg ha⁻¹ were 9.1%, 18.2%, and 33.33% in aeolian sandy soil and 3.0%, 15.25, and 21.2% in black soil when an optimized single irrigation was applied in late June or early July, respectively. Therefore, an optimized single irrigation should be applied in late June to early July with the irrigation amount to refill soil water storage of root zone to field capacity in CBNC.     

Breeding potential of Solanum tuberosum–S. commersonii pentaploid hybrids: fertility studies and tuber evaluation

Twenty-one (near) pentaploid hybrids between sexually incompatible Solanum commersonii [2x(1EBN)] and cultivated S. tuberosum [4x(4EBN)] were characterized for tuber traits and fertility. A number of genotypes resembled the S. tuberosum type in terms of stolon length and eye depth and produced tubers under long day conditions. Tuber yields were not as high as expected, probably due to lack of adaptation of the S. commersonii genome to the environmental conditions in southern Italy (on average 325 g·pt⁻¹ and 285 g·pt⁻¹ in 2003 and in 2004, respectively). Compared to 2004, in the summer of 2003, characterized by extremely high temperatures, hybrids gave a higher tuber yield than the S. tuberosum control, suggesting that in our environmental conditions the wild S. commersonii genome, rather than resulting in heterosis for tuber yield, provides better adaptation to harsh environments. Although aneuploidy has often been associated with reduced fertility, several hybrids were fertile in crosses with S. tuberosum when used as female parents. In particular, the average berry set and number of seeds per berry were 38.2% and 31.8%, respectively. Based on significant relationships between ploidy levels and all yield and fertility data measured, the presence of extra chromosome affected the parameters considered: tuber yield in 2003 and 2004, percentage of fruit set, number of seeds per berry and number of seeds per pollinated flower. Regression analysis also indicated that “Residuals” were significant for all parameters measured. Therefore, additional factors (e.g. the genetic makeup of hybrids) may be key to fertility and yield.     

Effect of Gluconacetobacter diazotrophicus and Trichoderma viride on soil health, yield and N-economy of sugarcane cultivation under subtropical climatic conditions of India

Intensive cropping and exhaustive nature of sugarcane–wheat–rice cropping system in the Indo-Gangetic Plains of South Asia have led to the depletion of soil organic carbon content and inherent soil fertility resulting in a serious threat to the sustainability of these production systems. Bioagents like Gluconacetobacter diazotrophicus and Trichoderma viride have great potential to restore soil fertility and promote sugarcane growth. Field experiments, therefore, have been conducted to study the integrated effect of bioagents (G. diazotrophicus and T. viride), Farm Yard Manure (FYM) and fertilizer N on sugarcane rhizosphere, crop yield and N economy for two crop cycles during 2004–2006 and 2005–2007 crop seasons at Lucknow, in the middle Indo-Gangetic plain region. Both bioagents could survive and colonize sugarcane rhizosphere and FYM improved their colonization. Enhanced soil microbial population and microbial carbon (SMC) and nitrogen (SMN) with increasing N level were probably due to more available N in the soil. FYM/bioagents amendment further enhanced the microbial carbon. The uniform increase in the fraction of SMC and SMN of total organic carbon indicated that immobilization/mineralization was being maintained in the soil where enhanced microbial biomass might act later as a source of nutrients.Bioagents ammended FYM enhanced the uptake of N, P and K in sugarcane at all the levels of fertilizer N. It was mainly due to the enhanced nutrient availability in the rhizospheric soil as the soil organic C and available N, P and K content increased with the application of bioagents/FYM. A saving of 76.3 kg N ha⁻¹ was envisaged by the use of G. diazotrophicus inoculated FYM with marginal (2.4 t ha⁻¹) decline in the cane yield. Application of T. viride enriched FYM, however, brought economy in the use of fertilizer N by 45.2 kg ha⁻¹ and also increased the yield by 6.1 t ha⁻¹compared to the control treatment. Overall, strategic planning in terms of an integrated application of these bioagents/manures with fertilizer N will not only sustain soil fertility but will also benefit farmers in terms of reducing their dependence and expenditure on chemical fertilizers.     

Radiation use efficiency and leaf photosynthesis of sweet corn in response to phosphorus in a cool temperate environment

Phosphorus (P) deficiency has been shown to decrease accumulated intercepted solar radiation (RI_cum) for sweet corn and in this paper the effects on radiation use efficiency (RUE) and leaf photosynthetic rate at 2000 μmol m⁻² s⁻¹ PPFD (P₂₀₀₀) are examined. Data from two consecutive field experiments on a low P site at Lincoln, New Zealand, were analyzed. In the first experiment (2001/2002) 0, 50, 100, 150 or 200 kg P ha⁻¹ was applied to sweet corn followed by an additional 0, 0, 10, 20 or 40 kg P ha⁻¹ in 2002/2003 applied to the same plots. Thus, total P applications were 0, 50, 110, 170, or 240 kg P ha⁻¹.There were no differences in RUE between P treatments but RUE changed with crop ontogeny. The RUE was 0.66 g MJ⁻¹ before each crop had 10 fully expanded leaves and RUE was 1.34 g MJ⁻¹ after this. The cause of this difference between development stages was unclear, but it was not related to air temperature. In contrast to RUE, there were clear differences in P₂₀₀₀ due to differences in specific leaf phosphorus (SLP). At a SLP of 0.12 g P m⁻² or greater, P₂₀₀₀ was a constant value of 34 μmol CO₂ m⁻² s⁻¹. When SLP was less than 0.12 g P m⁻² P₂₀₀₀ was reduced. The differences in P₂₀₀₀ between P treatments occurred when the plants were young (≤10 fully expanded leaves) and after this, SLP of all leaves exceeded 0.12 g P m⁻² and there were no differences in P₂₀₀₀. These early leaves were unimportant in determining crop RUE but are likely to have been important in establishing the hierarchies that led to the changes in RI_cum reported previously. These results demonstrate the importance of an adequate supply of P early in crop growth.     

Water relations and use-efficiency,plant survival and productivity of nine alfalfa (Medicago sativa L.) cultivars in dryland Mediterranean conditions

The potential for alfalfa to be a valuable rainfed pasture option that can be used to increase livestock and crop production in central-south Chile is demonstrated for the first time. The persistence, productivity and water use efficiency of alfalfa (Medicago sativa) cultivars was evaluated at four sites in dryland Mediterranean environments of Chile between 2012 and 2015. Nine alfalfa cultivars were evaluated for plant persistence, winter growth, forage yield, shoot water potential (Ψ), stomatal conductance (gs), and carbon (δ¹³C) and oxygen (δ¹⁸O) compositions. The percentage of plant survival after four years varied between 69 and 97% of the established plants. Alfalfa forage yield was >9 t ha⁻¹yr⁻¹ at Yungay (Andes foothills) in year two, and at Cauquenes (interior dryland site) in years three and four. The highest forage yield was obtained in cultivars SARDI Ten and Aquarius and the lowest in WL326HQ. In 2015, the forage yield during late spring and early summer (November 2015–January 2016) ranged from 3170 to 3570 kg ha⁻¹ (average of the nine cultivars), which represented between 29% and 38% of the total production. The response of gs to Ψ was different among cultivars but all cultivars exhibited a strong decline when Ψ was <−1.0 MP. Shoot water potential evaluated in October–November was negatively correlated with δ¹³C and δ¹⁸O in shoot biomass. The apparent water use efficiency (kg DM/ha/mm of rainfall) across the nine cultivars was highest in Cauquenes in the three years evaluated (>16 kg mm⁻¹). It is concluded that alfalfa is a high yielding forage that can extend the growing season into early summer and autumn in dryland Mediterranean environments.     

White lupine as a beneficial crop in Southern Europe. II. Nitrogen recovery in a legume–oat rotation and a continuous oat–oat

One experiment lasting for two years was carried out at Pegões (central Portugal) to estimate the impact of mature white lupine residue (Lupinus albus L.) on yield of fodder oat (Avena sativa L. cv. Sta. Eulalia) as the next crop in rotation, comparing with the continuous cultivation of cereal, under two tillage practices (conventional tillage and no-till) and fertilized with five mineral nitrogen (N) rates, with three replicates. Oat as a first crop in the rotation provided more N to the agro-ecosystem (63 kg N ha⁻¹) than did lupine (30–59 kg N ha⁻¹). This was at a cost of 100 kg of mineral N ha⁻¹, whereas lupine was grown without addition of N. A positive response of oat as a second crop was obtained per kg of lupine-N added to the system when compared with the continuous oat–oat. The cereal also responded positively to mineral N in the legume amended soil in contrast with the oat–oat sequence where no response was observed, partly due to the fast mineralization rate of lupine residue and a greater soil N immobilization in the continuous oat system. Each kg N ha⁻¹ added to the soil through the application of 73 kg DM ha⁻¹ mature lupine residue (above- and belowground material) increased by 72 kg DM ha⁻¹ the oat biomass produced as the second crop in rotation when 150 kg mineral N ha⁻¹ were split in the season, independent of tillage practice. Mature legume residue conserved in the no-tilled soil depressed the yield of succeeding cereal but less than the continuous oat–oat for both tillage practices, where the application of mineral N did not improve the crop response.     

Random amplified polymorphic DNA (RAPD) markers variability among cultivars and landraces of common beans (Phaseolus vulgaris L.) of south-Brazil

To evaluate the variability among cultivars and landraces of common bean(Phaseolus vulgaris L.), 15 cultivars and 18 landraces of common bean (Phaseolus vulgarisL.), a undefined species of Phaseolus,two landraces of Vigna angularis L., and a landrace of soybean (Glycine maxL.), were screened with fifteen oligonucleotide primers in PCR reactions. An average of 20.3 RAPD bands were scored per primer. A total of 304 amplification products were scored of which 88.8% were polymorphic among Phaseolus genotypes. Based on the RAPD markers, four major clusters were formed. Three clusters corresponded to the soybean, to the two Vigna angularis landraces, and to the Phaseolus sp. landrace, respectively. The fourth cluster include all the landraces and cultivars of Phaseolus vulgaris. This large group could be separated into three subgroups that were correlated with the phaseolin patterns and the average seed weight of the genotypes. The analysis shows that most of the landraces collected in South Brazil (17 out of 18) belong to the Andean gene pool, and most of the cultivars (13 out of 15) belong to the Middle American gene pool. This revised version was published online in July 2006 with corrections to the Cover Date.     

Participatory plant breeding approach for host plant resistance to bean fly in common bean under semi-arid Kenya conditions

Common bean (Phaseolus vulgaris L.) is the most important legume crop in Kenya. It is cultivated across a wide range of agro-ecologies which include high potential and marginal areas. Eastern Kenya alone, largely semi-arid, accounts for 35% of the country’s total bean production. Bean farmers mainly small-scale have limited access to quality seed, chemical pesticides and fertilizer. Therefore, bean yield under on-farm conditions still remains below 500 kg ha⁻¹ while the potential is about 1,200 kg ha⁻¹ under semi-arid conditions. To asses the farmers’ views on bean varieties and a key insect pest and associated constraints contributing to yield loss, research was undertaken. The research included survey to quantify the yield loss and Participatory Rural Appraisal to determine the level of adoption and criteria for variety choice in semi-arid eastern Kenya (SAEK). The results show that farmers consider drought and insect pest problems as main causes for low yields. The adoption rate for improved varieties is high but self sufficiency in beans stands at 23% in the dry transitional (DT) agro-ecology and at 18% in the dry mid-altitude (DM) agro-ecology, respectively. This could be attributed to low adaptability since most of the improved varieties grown were selected for high potential areas but now found in marginal areas. Drought, earliness, yield stability, and insect pest resistance are the main reasons for choice of varieties by farmers. Bean fly (Ophiomyia sp.) was identified as one of the key crop pests of beans limiting yield. Besides, African bollworm (Helicoverpa armigera) and bean aphid (Aphis fabae) were ranked higher. Due to limitations of the conventional breeding approach, a participatory plant breeding approach is suggested so as to provide an opportunity to develop insect pest resistant varieties adapted to the SAEK region.