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1.
To test the hypothesis that N isotope composition can be used as evidence of excessive compost application, we measured variation in patterns of N concentrations and corresponding δ15N values of plants and soil after compost application. To do so, a pot experiment with Chinese cabbage (Brassica campestris L. cv. Maeryok) was conducted for 42 days. Compost was applied at rates of 0 (SC0), 500 (SC1), 1000 (SC2), and 1500 mg N kg−1 soil (SC3). Plant-N uptake linearly increased with compost application (r2 = 0.956, P < 0.05) with an uptake efficiency of 76 g N kg−1 of compost-N at 42 days after application, while dry-mass accumulation did not show such linear increases. Net N mineralized from compost-N increased linearly (r2 = 0.998, P < 0.01) with a slope of 122 g N kg−1 of compost-N. Plant-δ15N increased curvilinearly with increasing compost application, but this increase was insignificant between SC2 and SC3 treatments. The δ15N of soil inorganic-N (particularly NO3-N) increased with compost application. We found that plant-δ15N reflected the N isotope signal of soil NO3-N at each measurement during plant growth, and that δ15N of inner leaves and soil NO3-N was similar when initial NO3 in the compost was abundant. Therefore, we concluded that δ15N of whole plant (more obviously in newer plant parts) and soil NO3-N could reveal whether compost application was excessive, suggesting a possible use of δ15N in plants and soil as evidence of excess compost application.  相似文献   

2.
An incubation experiment was conducted to study the response to sodium chloride (NaCl) salinity of microbial population immobilizing NH4+- and NO3-N using glucose as an easily oxidizable C source. Immobilization of NH4+-N was faster than that of NO3-N and was complete within 12 h of -incubation. Presence of NaCl retarded the process of N immobilization; that of NO3-N being more affected. Remineralization of immobilized N started within 48 h in case of both NH4+- and NO3-N and was faster for the latter. Both remineralization and nitrification were significantly delayed in the presence of NaCl; inhibition being more at 4000 mg NaCl kg−1 soil. The inhibitory effect of NaCl on remineralization of N was relatively more for NH4+-treated soil. The results of the study suggested a higher sensitivity to NaCl of microorganisms assimilating NO3. However, remineralization of N from NO3-assimilating microbial population was less affected by NaCl salinity compared to NH4+-assimilating population.  相似文献   

3.
Agricultural systems that receive high amounts of inorganic nitrogen (N) fertilizer in the form of either ammonium (NH4+), nitrate (NO3) or a combination thereof are expected to differ in soil N transformation rates and fates of NH4+ and NO3. Using 15N tracer techniques this study examines how crop plants and soil microbes vary in their ability to take up and compete for fertilizer N on a short time scale (hours to days). Single plants of barley (Hordeum vulgare L. cv. Morex) were grown on two agricultural soils in microcosms which received either NH4+, NO3 or NH4NO3. Within each fertilizer treatment traces of 15NH4+ and 15NO3 were added separately. During 8 days of fertilization the fate of fertilizer 15N into plants, microbial biomass and inorganic soil N pools as well as changes in gross N transformation rates were investigated. One week after fertilization 45-80% of initially applied 15N was recovered in crop plants compared to only 1-10% in soil microbes, proving that plants were the strongest competitors for fertilizer N. In terms of N uptake soil microbes out-competed plants only during the first 4 h of N application independent of soil and fertilizer N form. Within one day microbial N uptake declined substantially, probably due to carbon limitation. In both soils, plants and soil microbes took up more NO3 than NH4+ independent of initially applied N form. Surprisingly, no inhibitory effect of NH4+ on the uptake and assimilation of nitrate in both, plants and microbes, was observed, probably because fast nitrification rates led to a swift depletion of the ammonium pool. Compared to plant and microbial NH4+ uptake rates, gross nitrification rates were 3-75-fold higher, indicating that nitrifiers were the strongest competitors for NH4+ in both soils. The rapid conversion of NH4+ to NO3 and preferential use of NO3 by soil microbes suggest that in agricultural systems with high inorganic N fertilizer inputs the soil microbial community could adapt to high concentrations of NO3 and shift towards enhanced reliance on NO3 for their N supply.  相似文献   

4.
This study was conducted to examine whether the applications of N-inputs (compost and fertilizer) having different N isotopic compositions (δ15N) produce isotopically different inorganic-N and to investigate the effect of soil moisture regimes on the temporal variations in the δ15N of inorganic-N in soils. To do so, the temporal variations in the concentrations and the δ15N of NH4+ and NO3 in soils treated with two levels (0 and 150 mg N kg−1) of ammonium sulfate (δ15N=−2.3‰) and compost (+13.9‰) during a 10-week incubation were compared by changing soil moisture regime after 6 weeks either from saturated to unsaturated conditions or vice versa. Another incubation study using 15N-labeled ammonium sulfate (3.05 15N atom%) was conducted to estimate the rates of nitrification and denitrification with a numerical model FLUAZ. The δ15N values of NH4+ and NO3 were greatly affected by the availability of substrate for each of the nitrification and denitrification processes and the soil moisture status that affects the relative predominance between the two processes. Under saturated conditions for 6 weeks, the δ15N of NH4+ in soils treated with fertilizer progressively increased from +2.9‰ at 0.5 week to +18.9‰ at 6 weeks due to nitrification. During the same period, NO3 concentrations were consistently low and the corresponding δ15N increased from +16.3 to +39.2‰ through denitrification. Under subsequent water-unsaturated conditions, the NO3 concentrations increased through nitrification, which resulted in the decrease in the δ15N of NO3. In soils, which were unsaturated for the first 6-weeks incubation, the δ15N of NH4+ increased sharply at 0.5 week due to fast nitrification. On the other hand, the δ15N of NO3 showed the lowest value at 0.5 week due to incomplete nitrification, but after a subsequence increase, they remained stable while nitrification and denitrification were negligible between 1 and 6 weeks. Changing to saturated conditions after the initial 6-weeks incubation, however, increased the δ15N of NO3 progressively with a concurrent decrease in NO3 concentration through denitrification. The differences in δ15N of NO3 between compost and fertilizer treatments were consistent throughout the incubation period. The δ15N of NO3 increased with the addition of compost (range: +13.0 to +35.4‰), but decreased with the addition of fertilizer (−10.8 to +11.4‰), thus resulting in intermediate values in soils receiving both fertilizer and compost (−3.5 to +20.3‰). Therefore, such differences in δ15N of NO3 observed in this study suggest a possibility that the δ15N of upland-grown plants receiving compost would be higher than those treated with fertilizer because NO3 is the most abundant N for plant uptake in upland soils.  相似文献   

5.
We studied controls on nitrification in an undisturbed water-limited ecosystem by inhibiting autotrophic nitrifying bacteria in soils with varying levels of vegetative cover. The activity of nitrifying bacteria was disrupted using nitrapyrin, 2-chloro-6-(trichloromethyl)-pyridine, under field conditions in three microenvironments (underneath shrubs, next to grasses and in bare soil). Ammonia-oxidising bacteria were detected by PCR analysis of DNA in soils. The inhibition of nitrification changed the concentrations of NO3 and NH4+ in the soil, while the microenvironment was most important in determining the response of bacteria to the inhibitor. Nitrapyrin application resulted in a significant (p<0.05) reduction in soil NO3 concentration (39%) and a significant increase (p<0.001) in soil NH4+ concentration (41%). Untreated bare-soil microenvironments had the lowest concentrations of NH4+ (1.57 μg/g of dry soil) and NO3 (0.49 μg/g of dry soil) when compared to the other microenvironments, and showed the highest impacts of nitrification inhibition. For example, NH4+ concentrations increased 288% and NO3 concentrations decreased 60% in inhibited bare-soil microenvironments. In contrast, untreated microenvironments underneath shrubs had the highest levels of NH4+ (10.01 μg/g of dry soil) and NO3 (0.69 μg/g of dry soil), but showed no significant effects of inhibition of nitrification on soil nitrogen concentrations.  相似文献   

6.
The soil of the former lake Texcoco is an ‘extreme’ alkaline saline soil with pH > 10 and electrolytic conductivity (EC) > 150 dS m−1. These conditions have created a unique environment. Application of wastewater sludge to Texcoco soil showed that large amounts of NH4+ were immobilized, NO3 was reduced aerobically, NO2 was formed and the mineralization of the organic material in the sludge was inhibited. A series of experiments were initiated to study the processes that inhibited the decomposition of organic material and affected the dynamics of mineral N. The large EC and pH inhibited the decomposition of easily decomposable organic material such as glucose and maize, although cellulolytic activity was observed in soil with pH 9.8 and EC 32.7 dS m−1. The high soil pH favoured NH3 volatilization of approximately 50 mg N kg−1 soil within a day and a similar amount could be fixed on the soil matrix due to the dispersed minerals and their volcanic origin. Soil microorganisms immobilized large amounts of NH4+ within a day when glucose was added to soil in excess of what was required for metabolic activity. Removal of NO3 from soil amended with glucose was not inhibited by 100% O2 and NH4+ indicating that the contribution of denitrification and assimilatory reduction to the reduction of NO3 was minimal while the formation of NO2 was not inhibited by 0.1% acetylene, known to inhibit nitrification. Additionally, the reduction of NO3 in the glucose-amended alkaline saline Texcoco soil was followed by an increase in the amount of NH4+, which could not be due to denitrification. It was concluded that the reduction of NO3 and the formation of NO2 and NH4+ in the glucose-amended soil was a result of aerobic NO3 reduction. A phylogenetic analysis of the archaeal community in the soil of the former lake Texcoco showed that some of the clones identified were capable of reducing NO3 aerobically to NO2 when glucose was added. A study of the diversity of the bacterial dissimilatory and respiratory nitrate-reducing communities indicated that bacteria could have contributed to the process.  相似文献   

7.
Burning of the vegetation in the African savannahs in the dry season is widespread and may have significant effects on soil chemical and biological properties. A field experiment in a full factorial randomised block design with fire, ash and extra grass biomass as main factors was carried out in savannah woodland of the Gambella region in Ethiopia. The microbial biomass C (Cmic) was 52% (fumigation-extraction) and 20% (substrate-induced respiration) higher in burned than unburned plots 12 d after burning. Both basal respiration and potential denitrification enzyme activity (PDA) immediately responded to burning and increased after treatment. However, in burned plots addition of extra biomass (fuel load) led to a reduction of Cmic and PDA due to enhanced fire temperature. Five days after burning, there was a short-lived burst in the in situ soil respiration following rainfall, with twice as high soil respiration in burned than unburned plots. In contrast, 12 d after burning soil respiration was 21% lower in the burned plots, coinciding with lower soil water content in the same plots. The fire treatment resulted in higher concentrations of dissolved organic C (24-85%) and nitrate (47-76%) in the soil until 90 d after burning, while soil NH4+-N was not affected to the same extent. The increase in soil NO3-N but not NH4+-N in the burned plots together with the well-aerated soil conditions indicated that nitrifying bacteria were stimulated by fire and immediately oxidised NH4+-N to NO3-N. In the subsequent rainy season, NO3-N and, consequently, PDA were reduced by ash deposition. Further, Cmic was lower in burned plots at that time. However, the fire-induced changes in microbial biomass and activity were relatively small compared to the substantial seasonal variation, suggesting transient effects of the low severity experimental fire on soil microbial functioning.  相似文献   

8.
The effects of animal treading on denitrification in a mixed ryegrass-clover pasture were studied. A single treading event of moderate or severe intensity was applied in plots during spring by using dairy cows at varying stocking rates (4.5 cows 100 m−2 for 1.5 or 2.5 h, respectively). Treading caused a significant short-term 21 days) increase in denitrification. Denitrification rates reached a maximum of 52 g N2O-N ha−1 day−1 at 8 days after severe treading compared to 2.3 g N2O-N ha−1 day−1 under nil treading. Thereafter, denitrification rates declined, and were similar to non-trodden control plots after 28 days. Soil aeration, was significantly reduced by treading as expressed by water-filled porosity. In addition, soil NH4+-N and NO3-N concentrations were also increased by treading. We propose that the underlying processes involved in increasing denitrification under treading were two-fold. Firstly, treading caused a temporary (e.g. 3 days after treading) reduction in soil aeration through soil physical damage, and secondly, reduced soil N utilisation prompted by reduced plant growth led to increased soil NH4+-N and NO3-N availability. This study shows that treading, without the influence of other grazing animal factors (e.g. excretion), can cause a large short-term stimulation of denitrification in grass-clover pastures.  相似文献   

9.
The short-term effects of excessive NH4+-N on selected characteristics of soil unaffected (low annual N inputs) and affected (high annual N inputs) by cattle were investigated under laboratory conditions. The major hypothesis tested was that above a theoretical upper limit of NH4+ concentration, an excess of NH4+-N does not further increase NO3 formation rate in the soil, but only supports accumulation of NO2-N and gaseous losses of N as N2O. Soils were amended with 10 to 500 μg NH4+-N g−1 soil. In both soils, addition of NH4+-N increased production of NO3-N until some limit. This limit was higher in cattle-affected soil than in unaffected soil. Production of N2O increased in the whole range of amendments in both soils. At the highest level of NH4+-N addition, NO2-N accumulated in cattle-affected soil while NO3-N production decreased in cattle-unaffected soil. Despite being statistically significant, observed effects of high NH4+-N addition were relatively weak. Uptake of mineral N, stimulated by glucose amendment, decreased the mineral N content in both soils, but it also greatly increased production of N2O.  相似文献   

10.
Temporal variations in δ15N of NH4+ and NO3 in water-saturated and unsaturated soils were examined in a laboratory incubation study. Ammonium sulfate (δ15N=−2.6‰) was added to 25 g samples of soil at concentrations of 160 mg N kg−1. Soils were then incubated under unsaturated (50% of water holding capacity at saturation, WHC) or saturated (100% of WHC) water conditions for 7 and 36 d, respectively. During 7 d incubation of unsaturated soil, the NH4+-N concentration decreased from 164.8 to 34.4 mg kg−1, and the δ15N of NH4+ increased from −0.4 to +57.2‰ through nitrification, as evidenced by corresponding increase in NO3-N concentration and lower δ15N of NO3 (product) than that of NH4+ (substrate) at each sampling time. In saturated soil, the concentration of NH4+-N decreased gradually from 162.4 to 24.2 mg kg−1, and the δ15N values increased from +0.8 to +21.0‰ during 36 d incubation. However, increase in NO3 concentration was not observed due to loss of NO3 through concurrent denitrification in anaerobic sites. The apparent isotopic fractionation factors (αs/p) associated with decrease in NH4+ concentration were 1.04 and 1.01 in unsaturated and saturated soils, respectively. Since nitrification is likely to introduce greater isotope fractionation than microbial immobilization, the higher value for unsaturated soil probably reflected faster nitrification under aerobic conditions. The lower value for saturated soil suggests that immobilization and subsequent remineralization of NH4+ were relatively more dominant than nitrification under the anaerobic conditions.  相似文献   

11.
Field data have shown that soil nitrifying communities gradually adapt to zinc (Zn) after a single contamination event with reported adaptation times exceeding 1 year. It was hypothesized that this relatively slow adaptation relates to the restricted microbial diversity and low growth rate of the soil nitrifying community. This hypothesis was tested experimentally by recording adaptation rates under varying nitrification activities (assumed to affect growth rates) and by monitoring shifts in community composition. Soils were spiked at various Zn concentrations (0-4000 mg Zn kg−1) and two NH4+-N doses (N1, N2) were applied to stimulate growth. A control series receiving no extra NH4+-N was also included. Soils were incubated in pots under field conditions with free drainage. The pore water Zn concentration at which nitrification was halved (EC50, mg Zn l−1) did not change significantly during 12 months in the control series (without NH4+-N applications), although nitrification recovered after 12 months at the highest Zn dose only. The EC50 after 12 months incubation increased by more than a factor 10 with increasing NH4+-N dose (p < 0.05) illustrating that increased activity accelerates adaptation to Zn. Zinc tolerance tests confirmed the role of Zn exposure, time and NH4+-N dose on adaptation. Zinc tolerance development was ascribed to the AOB community since the AOB/AOA ratio (AOB = ammonia oxidizing bacteria; AOA = ammonia oxidizing archaea) increased from 0.4 in the control to 1.4 in the most tolerant community. Moreover, the AOB amoA DGGE profile changed during Zn adaptation whereas the AOA amoA DGGE profile remained unaffected. These data confirm the slow but pronounced adaptation of nitrifiers to Zn contamination. We showed that adaptation to Zn was accelerated at higher activity and was associated with a shift in soil AOB community that gradually dominated the nitrifying community.  相似文献   

12.
Legumes increase the plant-available N pool in soil, but might also increase NO3 leaching to groundwater. To minimize NO3 leaching, N-release processes and the contribution of legumes to NO3 concentrations in soil must be known. Our objectives were (1) to quantify NO3-N export to >0.3 m soil depth from three legume monocultures (Medicago x varia Martyn, Onobrychis viciifolia Scop., Lathyrus pratensis L.) and from three bare ground plots. Furthermore, we (2) tested if it is possible to apply a mixing model for NO3 in soil solution based on its dual isotope signals, and (3) estimated the contribution of legume mineralization to NO3 concentrations in soil solution under field conditions. We collected rainfall and soil solution at 0.3 m soil depth during 1 year, and determined NO3 concentrations and δ15N and δ18O of NO3 for >11.5 mg NO3-N l−1. We incubated soil samples to assess potential N release by mineralization and determined δ15N and δ18O signals of NO3 derived from mineralization of non-leguminous and leguminous organic matter.Mean annual N export to >0.3 m soil depth was highest in bare ground plots (9.7 g NO3-N m−2; the SD reflects the spatial variation) followed by Medicago x varia monoculture (6.0 g NO3-N m−2). The O. viciifolia and L. pratensis monocultures had a much lower mean annual N export (0.5 and 0.3 g NO3-N m−2). The averaged NO3-N leaching during 70 days was not significantly different between field estimates and incubation for the Medicago x varia Martyn monoculture.The δ15N and δ18O values in NO3 of rainfall (δ15N: 3.3±0.8‰; δ18O: 30.8±4.7‰), mineralization of non-leguminous SOM (9.3±0.9‰; 6.7±0.8‰), and mineralization of leguminous SOM (1.5±0.6‰; 5.1±0.9‰) were markedly different. Applying a linear mixing model based on these three sources to δ15N and δ18O values in NO3 of soil solution during winter 2003, we calculated 18-41% to originate from rainfall, 38-57% from mineralization of non-leguminous SOM, and 18-40% from mineralization of leguminous SOM.Our results demonstrate that (1) even under legumes NO3-N leaching was reduced compared to bare ground, (2) the application of a three-end-member mixing model for NO3 based on its dual isotope signals produced plausible results and suggests that under particular circumstances such models can be used to estimate the contributions of different NO3 sources in soil solution, and (3) in the 2nd year after establishment of legumes, they contributed approximately one-fourth to NO3-N loss.  相似文献   

13.
Changes of land-use type (LUT) can affect soil nutrient pools and cycling processes that relate long-term sustainability of ecosystem, and can also affect atmospheric CO2 concentrations and global warming through soil respiration. We conducted a comparative study to determine NH4+ and NO3 concentrations in soil profiles (0–200 cm) and examined the net nitrogen (N) mineralization and net nitrification in soil surface (0–20 cm) of adjacent naturally regenerated secondary forests (NSF), man-made forests (MMF), grasslands and cropland soils from the windy arid and semi-arid Hebei plateau, the sandstorm and water source area of Beijing, China. Cropland and grassland soils showed significantly higher inorganic N concentrations than forest soils. NO3-N accounted for 50–90% of inorganic N in cropland and grassland soils, while NH4+-N was the main form of inorganic N in NSF and MMF soils. Average net N-mineralization rates (mg kg1 d1) were much higher in native ecosystems (1.51 for NSF soils and 1.24 for grassland soils) than in human disturbed LUT (0.15 for cropland soils and 0.85 for MMF soils). Net ammonification was low in all the LUT while net nitrification was the major process of net N mineralization. For more insight in urea transformation, the increase in NH4+ and, NO3 concentrations as well as C mineralization after urea addition was analyzed on whole soils. Urea application stimulated the net soil C mineralization and urea transformation pattern was consistent with net soil N mineralization, except that the rate was slightly slower. Land-use conversion from NSF to MMF, or from grassland to cropland decreased soil net N mineralization, but increased net nitrification after 40 years or 70 years, respectively. The observed higher rates of net nitrification suggested that land-use conversions in the Hebei plateau might lead to N losses in the form of nitrate.  相似文献   

14.
《Applied soil ecology》2011,47(3):321-328
Biogenic structures produced by soil ecosystem engineers influence the soil architecture and mediate soil functions and ecosystem services. Ant mounds in meadow wetlands are important biogenic structures with the potential of altering carbon storage and nutrient cycling in these ecosystems. In this study, we examined the soil nutrient concentrations of ant mounds and their effects on the wetland nutrient storage functions in meadow wetlands of the Sanjiang Plain, in northeastern China. The aims of this study were to investigate C, N and P variation in active ant mounds produced by Formica sanguinea Latreille and Lasius flavus Fabricius to estimate the C, N and P pools of ant mounds in comparison with control soil. The average total N (TN), total P (TP) and available P (AP) concentrations in the ant mounds of both species were higher than in the control soil. Organic carbon (Corg), DOC, NH4+ and NO3 in F. sanguinea mounds were higher than in the control soil, but not for L. flavus mounds. Average concentrations of all the five types of nutrient were higher in F. sanguinea mounds than in L. flavus mounds. The variations in Corg, DOC, TN and TP concentrations in ant mounds were not significant at depths from 0 to 25 cm. NH4+ and NO3 concentrations differed by soil layers for F. sanguinea mounds but not for L. flavus mounds. The C/N ratios were generally lower in the mounds than in the control soil (at 5–25 cm), but no significant differences were found for C/P ratios (except at 10–15 cm). Carbon and DOC pools were smaller, TN and AP pools were larger in ant mounds compared with the control soil, but there was no significant difference for TP pools. NH4+and NO3 pools were substantially larger in F. sanguinea mounds, but smaller in L. flavus mounds, than those in the control soil. All of the five types of nutrient pools were larger in F. sanguinea than in L. flavus mounds. Ant mounds increased the spatial variability of soil nutrient pools in the wetland.  相似文献   

15.
Coastal areas in the southeastern USA are prone to hurricanes and strong storms that may cause salt-water influx to freshwater aquatic sediments. These changes in environmental conditions may impact sediment processes including nitrogen (N) cycling. The relative abilities of sediment microbial communities from two freshwater golf course retention ponds that drain into the adjacent wetlands, and two proximal saline wetland ponds, to remove nitrate (NO3) were compared to assess whether low concentrations of sulfide changed N-transformation processes. Microcosms were incubated with NO3-N (300 μg g dw−1) alone, and with NO3-N and sulfide (H2S) (100 and 200 μg g dw−1). Nitrous oxide (N2O), nitrite (NO2), NO3, ammonium (NH4+), SO42− and acid volatile sulfides were analyzed over time. The acetylene block technique was used to measure denitrification in sediment microcosms with no added H2S. Denitrification was measured without acetylene (C2H2) addition in microcosms with added H2S. With no added H2S, denitrification was greater in the freshwater retention ponds than in the wetland ponds. Although low H2S concentrations generally increased NO3-N removal rates at all sites, lag periods were increased and denitrification was inhibited by low sulfide in the freshwater sediments, as evidenced by the greater concentrations of N2O that accumulated compared to those in the wetland sediments. In addition to the inability of the freshwater sediments to convert N2O to N2 in the absence of C2H2, anomalously high transient NO2-N concentrations accumulated in the retention pond samples. NH4-N formation generally decreased due to H2S addition at the freshwater sites; NH4-N formation increased initially at the wetland sites, but was greater when no H2S was added. Storm events that allow influx of SO42−-containing seawater into freshwater systems may change the dominant N species produced from nitrate reduction. Even low concentrations of sulfide produced incomplete denitrification and decreased formation of NH4+ in these coastal freshwater sediments.  相似文献   

16.
We examined effects of wetting and then progressive drying on nitrogen (N) mineralization rates and microbial community composition, biomass and activity of soils from spinifex (Triodia R. Br.) grasslands of the semi-arid Pilbara region of northern Australia. We compared soils under and between spinifex hummocks and also examined impacts of fire history on soils over a 28 d laboratory incubation. Soil water potentials were initially adjusted to −100 kPa and monitored as soils dried. We estimated N mineralization by measuring changes in amounts of nitrate (NO3-N) and ammonium (NH4+-N) over time and with change in soil water potential. Microbial activity was assessed by amounts of CO2 respired. Phospholipid fatty acid (PLFA) analyses were used to characterize shifts in microbial community composition during soil drying. Net N mineralized under hummocks was twice that of open spaces between hummocks and mineralization rates followed first-order kinetics. An initial N mineralization flush following re-wetting accounted for more than 90% of the total amount of N mineralized during the incubation. Initial microbial biomass under hummocks was twice that of open areas between hummocks, but after 28 d microbial biomass was<2 μ g−1 ninhydrin N regardless of position. Respiration of CO2 from soils under hummocks was more than double that of soils from between hummocks. N mineralization, microbial biomass and microbial activity were negligible once soils had dried to −1000 kPa. Microbial community composition was also significantly different between 0 and 28 d of the incubation but was not influenced by burning treatment or position. Regression analysis showed that soil water potential, microbial biomass N, NO3-N, % C and δ15N all explained significant proportions of the variance in microbial community composition when modelled individually. However, sequential multiple regression analysis determined only microbial biomass was significant in explaining variance of microbial community compositions. Nitrogen mineralization rates and microbial biomass did not differ between burned and unburned sites suggesting that any effects of fire are mostly short-lived. We conclude that the highly labile nature of much of soil organic N in these semi-arid grasslands provides a ready substrate for N mineralization. However, process rates are likely to be primarily limited by the amount of substrate available as well as water availability and less so by substrate quality or microbial community composition.  相似文献   

17.
Enchytraeids are involved both directly and indirectly in decomposition processes and nitrogen mineralization in soil. Their influence is especially important in nitrogen poor ecosystems such as heathland where the enchytraeid species, Cognettia sphagnetorum, is often abundant and playing a significant role in the N-cycling. The objective of this study was to quantify NH4+-N excretion of C. sphagnetorum at different temperatures. The results were combined with investigations of population dynamics during one year to estimate annual NH4+-N excretion of the population of C. sphagnetorum in a dry Danish heath soil. C. sphagnetorum significantly increased its NH4+-N excretion rate with increasing temperature. At 5 °C about 0.5 μg NH4+-N mg dry weight−1 day−1 was excreted increasing to about 3.3 μg NH4+-N mg dry weight−1 day−1 at 20 °C. Average enchytraeid biomass in the field was 2.5-3.5 g dry weight m−2 during cool and wet periods. Dry and warm conditions in May and June, 2008, had a drastic and long-term negative impact on the enchytraeid community. The excretion of NH4+-N by enchytraeids was therefore highest during the cool and moist months despite low temperatures (October 2007-May 2008) and amounted to about 2 mg NH4+-N m−2 day−1 during this period. The estimated annual NH4+-N excretion of the enchytraeid community was approximately 0.3 g N m−2 year−1. The results of the present study and the method described for estimation of N-excretion can increase our understanding of enchytraeids’ role in nitrogen mineralization.  相似文献   

18.
Ant mounds often occur at high densities in marsh wetlands. However, little information is available regarding their impacts on soil nutrient pools in these ecosystems. We studied Corg, dissolved organic carbon (DOC), total nitrogen (TN), NO3 and NH4+ concentrations in above-ground ant mounds and in soils under mounds for three ant species (Lasius flavus, Lasius niger and Formica candida), and estimated their contribution to the total soil nutrient pools in a marsh wetland. Ant impacts were greatest in above-ground soils. All measured nutrient concentrations in above-ground mounds were significantly higher than the average values in reference soils (upper 25 cm). However, except for DOC, no significant differences for nutrient concentrations existed between soils under mounds and reference soils. The impacts of ant mounds on soil C and nutrient concentrations varied by ant species. L. niger above-ground mounds stored less Corg, TN and NO3 than F. candida and L. flavus mounds, or reference soils. At the ecosystem scale, soils in above-ground mounds and under ant mounds all contained less Corg per hectare than the reference soils. Total amounts in nutrient pools from mounds of the three ant species comprised from 5.3% to 7.6% of the total in natural marsh soils. More importantly, ant mounds increased the spatial heterogeneity of nutrient pools. Thus, ant mounds can be important to a fully integrated understanding of the structure and function of wetland nutrient cycles and balances.  相似文献   

19.
Abstract

A sandy soil was amended with various rates (20 – 320 g air-dry weight basis of the amendments per kg of air-dry soil) of chicken manure (CM), sewage sludge (SS), and incinerated sewage sludge (ISS) and incubated for 100 days in a greenhouse at 15% (wt/wt) soil water content. At the beginning of incubation, NH4-N concentrations varied from 50 – 280 mg kg?1 in the CM amended soil with negligible amounts of NO3-N. Subsequently, the concentration of NH4-N decreased while that of NO3-N increased rapidly. In soil amended with SS at 20 – 80 g kg?1 rates, the NO3-N concentration increased sharply during the first 20 days, followed by a slow rate of increase over the rest of the incubation period. However, at a 160 g kg?1 SS rate, there were three distinct phases of NO3-N release which lasted for160 days. In the ISS amended soil, the nitrification process was completed during the initial 30 days, and the concentrations of NH4-N and NO3-N were lower than those for the other treatments. The mineralized N across different rates accounted for 20 – 36%, 16 – 40%, and 26 – 50% of the total N applied as CM, SS, and ISS, respectively.  相似文献   

20.
Alongside nitrate, dissolved organic nitrogen (DON) represents a significant N loss pathway in many agroecosystems. To better understand the factors controlling DON leaching in soil we followed the vertical movement of 15N-labeled NO3, NH4+, alanine and trialanine in packed soil columns in response to a simulated rainfall event. We show that in autoclaved (sterile) soil where sorption is assumed to be the dominant regulating factor, leaching followed the series NO3 > trialanine > alanine > NH4+. In the non-sterile packed soil columns, the rapid rate of NO3 leaching was unaffected whilst the movement of the amino acid, peptide and NH4+ was almost completely prevented due to microbial immobilization. Our results support the view that (1) DON loss from agricultural soils occurs mainly in the form of recalcitrant compounds (e.g. humic DON) rather than in the form of labile low MW DON (e.g. oligopeptides and amino acids), and (2) that although nitrate was bioavailable, it was not a preferred N form for the C-limited microbial biomass.  相似文献   

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