共查询到17条相似文献,搜索用时 234 毫秒
1.
大鼠乳腺局部免疫的调节机制--泌乳期与静止期乳腺肥大细胞分布的比较 总被引:6,自引:1,他引:5
应用改良甲苯胺蓝染色法(MTB)观察了大鼠泌乳期和静止期乳腺肥大细胞的分布、形态、数量变化规律.并用阿尔新蓝一番红鉴别染色法(AB-S)进行了细胞化学分型研究。结果:AB-S染色显示,大鼠乳腺只存在黏膜型肥大细胞;无论是泌乳期还是静止期。乳腺肥大细胞大多分布于腺泡间和小叶问结缔组织中。细胞的形态各异,但细胞数量在静止期和泌乳期有显著差异。泌乳期的乳腺肥大细胞数量明显减少(P<0.01)。乳腺肥大细胞的动态变化,可能与乳腺泌乳期腺泡上皮的生长和静止期腺泡问结缔组织细胞增生等结构变化有关。 相似文献
2.
《黑龙江畜牧兽医》2014,(19)
为了研究奶牛乳腺肥大细胞的数量与奶牛乳腺胶原纤维面积的相关性,试验对奶牛泌乳期和静止期乳腺进行采样,应用甲苯胺蓝组织化学染色法观察奶牛泌乳期和静止期乳腺肥大细胞数量的变化特点,应用苦味酸天狼猩红染色法观察奶牛泌乳期和静止期乳腺胶原纤维面积的变化特点。结果表明:静止期奶牛乳腺肥大细胞的数量明显比泌乳期多(P0.01),静止期奶牛乳腺胶原纤维的面积明显比泌乳期大(P0.01)。静止期奶牛乳腺肥大细胞的数量多,胶原纤维的面积也大;泌乳期的奶牛乳腺肥大细胞的数量少,胶原纤维的面积也小。说明奶牛乳腺肥大细胞的数量与胶原纤维的面积存在明显正相关性(r=0.677,P0.05)。 相似文献
3.
4.
5.
为了探讨不同固定液对鸭小肠肥大细胞甲苯胺蓝染色效果的影响及鸭小肠内肥大细胞的数量和分布情况,试验选择5只100日龄的健康淮南麻鸭公鸭为研究对象,取鸭只十二指肠、空肠和回肠组织样品,均分为两份,分别置于10%中性福尔马林固定液和卡恩氏(Carnoy)固定液中固定,常规制作石蜡切片,切片经甲苯胺蓝染色后,观察小肠肥大细胞染色效果和肥大细胞形态、数量及分布,并进行统计分析。结果表明:Carnoy固定液对肥大细胞的染色效果优于10%中性福尔马林固定液,染色评分极显著高于10%中性福尔马林固定液(P<0.01);Carnoy固定液固定的十二指肠、空肠、回肠肥大细胞数量均极显著高于10%中性福尔马林固定液(P<0.01)。鸭十二指肠、空肠和回肠中肥大细胞形态和数目不同,在黏膜固有层血管、腺管周围的结缔组织中分布较多;空肠中肥大细胞数量最多,形态多样;十二指肠和回肠中肥大细胞数量递减,形态多为圆形。说明与10%中性福尔马林固定液相比,经Carnoy固定液固定的鸭小肠组织染色后,可清晰显示肥大细胞的形态分布。 相似文献
6.
7.
将绒山羊的空肠用Carnoy氏液、Bouin氏液、4%的中性甲醛(NBF)及4%的多聚甲醛分别固定,然后进行常规甲苯胺蓝-藏红O复染法(MTB-S)和改良甲苯胺蓝-藏红O复染法(LTB-S)染色,光镜观察,探讨不同的固定液和染色方法对显示绒山羊空肠肥大细胞(MC)的影响。结果:绒山羊空肠组织采用Carnoy氏液固定,甲苯胺蓝-藏红O复染法和改良甲苯胺蓝-藏红O复染法对所有的肥大细胞均有较好的染色效果,而Bouin氏液、4%的中性甲醛(NBF)及4%的多聚甲醛固定的空肠,只有少量的MC显示。说明MTB-S与LTB-S均是绒山羊肥大细胞较好的染色方法,固定液配Carnoy氏液为佳。 相似文献
8.
《黑龙江畜牧兽医》2010,(21)
为了对奶牛乳腺肥大细胞的组织化学特点和分布特点进行研究,试验对取自21头发育各期奶牛的乳腺样品,应用免疫组织化学方法(SP)观察奶牛乳腺肥大细胞5-羟色胺(5-HT)和类胰蛋白酶的表达。结果表明:5-羟色胺和类胰蛋白酶在奶牛乳腺肥大细胞中表达,肥大细胞数量在各期有显著差异,泌乳期的乳腺肥大细胞数量明显减少(P0.05),分娩后60天奶牛乳腺肥大细胞的数量最少;5-羟色胺在不同发育时期奶牛乳腺组织中的表达量少于类胰蛋白酶在不同发育时期奶牛乳腺组织中的表达量。说明肥大细胞的数量随生理周期的改变而增减的趋势可能与乳腺的发育、乳腺局部免疫状态及内分泌调节有密切关系。 相似文献
9.
10.
11.
泌乳周期中外源物质对大鼠乳腺内肥大细胞数量的影响及乳腺组织内组胺含量的研究 总被引:1,自引:1,他引:1
选择泌乳期和静止期大鼠,经乳腺局部(乳导管)或外周(跖部皮下)注射辣根过氧化物酶(HRP)后,应用改良甲苯胺蓝染色法(MTB)对乳腺及其引流(鼠蹊部)和非引流(腘)淋巴结内的肥大细胞数量变化进行了研究,用荧光测定改良法检测了乳腺组织中组胺含量。结果显示,对照组乳腺及引流和非引流淋巴结内的肥大细胞数量均为静止期显著多于泌乳期(P〈0.01),乳腺中组胺含量也呈相同的变化趋势。在泌乳期从不同部位引人HRP后,乳腺及乳腺引流淋巴结内肥大细胞数量明显增多,与对照组相比差异极显著(P〈0.01);而乳腺非引流淋巴结内肥大细胞数量则略有减少,与对照组相比差异不显著(P〉0.05)。在静止期从不同部位引人HRP后,乳腺及乳腺非引流淋巴结内肥大细胞数均减少,而乳腺引流淋巴结内的肥大细胞数则随引人部位不同而有差别:乳腺引人时增加,而外周引入时减少。本研究结果表明肥大细胞数量随生理周期的改变而增减的趋势与乳腺的发育、乳腺局部免疫状态及内分泌调节都有关系,而且不同泌乳阶段乳腺内肥大细胞对异物的易感性也存在差异。 相似文献
12.
Onda K Matsuki N Ono K Wada Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(1):9-13
The expression of parathyroid hormone-related protein (PTHrP) mRNA was examined in mammary gland with or without lactation, and during periparturient period in a Holstein cow and a Jersey cow. In the lactating mammary gland, PTHrP was detected in alveolar epithelial cells and the lumen by immunohistochemical analysis. The relative expression levels of PTHrP mRNA in mammary gland from lactating cows were significantly higher than those from non-lactating cows (P<0.05). During periparturient period, relative PTHrP mRNA level was remarkably low before the parturition in a Jersey and a Holstein cow, however, both levels were gradually increased and reached a peak level at 5-6 weeks after the parturition. In addition, the peak level in a Jersey cow was approximately 3-fold higher than that in a Holstein cow. From these results, PTHrP was synthesized and secreted in alveolar epithelial cells in mammary gland and increased subsequently with the lactation, suggesting a possible mechanism for the regulation of local calcium homeostasis. 相似文献
13.
目的:试验旨在通过对6月龄剑白香猪肥大细胞的观察,以期了解肥大细胞在剑白香猪不同组织器官的数量、分布及形态。方法:采集剑白香猪心、肝、脾、肺、肾、肾上腺、小肠等部位样品用多聚甲醛和carnoy’s液固定后,用甲苯胺蓝染色法检测各组织中肥大细胞数量、分布及形态,并用SPSS13.0软件进行统计分析。结果:试验表明,肥大细胞数量在不同性别问无明显差异;Carnoy’S固定液是测定猪各组织器官MC的较理想固定液;肥大细胞在各种组织中分布及形态复杂多样。本试验可为剑白香猪肥大细胞的进一步研究提供参考数据。 相似文献
14.
The distribution, density and histochemical characteristics of mast cells in the lungs of the Japanese quail were investigated during the post-hatching period. In the period starting from the first to the 60th day post-hatching, based on proteoglycan content, three types of mast cells, which were alcian blue-positive, safranin O-positive and alcian blue/safranin O-positive, were found to exist in the lungs. The application of staining with berberine sulphate demonstrated that, similar to the distribution of safranin O-positive cells, the heparin-containing cells were located in the periphery of large blood vessels. The percentages of mast cells in different localization sites of the lungs were found to vary with age in the post-hatching period with toluidine blue staining. The lack of any statistically significant increase/decrease in the number of mast cells per unit area of the right and left lung lobes is partially in favour of the proposal that the mast cell number increases with the growth of the lung volume in the post-hatching period. 相似文献
15.
LIN Lin FAN Hui-quan XING Wei-nan YANG Yang HOU Xiao-ming ZHANG Chang-shun LIN Ye 《中国畜牧兽医》2015,42(5):1240-1244
To investigate the relationship between the expression of SYK and dairy cow mammary gland development and lactation, the expression of SYK in lactating dairy cow mammary gland with high or low quality milk and dry period Holstein dairy cow mammary gland was detected by Western blotting and laser confocal microscope.The results showed that SYK expression in dry period mammary gland was significant higher than that in lactating mammary gland (P<0.05).There was no SYK differential expression detected between lactating mammary gland with high quality milk and low quality milk (P>0.05).SYK was mainly located in the cytoplasm of ductal epithelial cells in dry period mammary gland.In lactating mammary gland, SYK was existed in acinar epithelial cells.All these results revealed that SYK was a regulator in mammary epithelial cell proliferation and differentiation.It participated in mammary gland reconstitution in dry period. 相似文献
16.
为探讨脾源性酪氨酸激酶(spleen tyrosine kinase,SYK)的表达与奶牛乳腺发育和泌乳功能之间的关系,试验采用Western blotting和激光共聚焦显微技术对泌乳期高乳品质、低乳品质及干乳期的中国荷斯坦奶牛乳腺组织中SYK的表达含量和表达部位的变化进行研究。结果表明,干乳期奶牛乳腺组织中SYK的表达显著高于泌乳期奶牛乳腺组织(P<0.05),泌乳期高乳品质、低乳品质奶牛乳腺组织中SYK的表达差异不显著(P>0.05);在干乳期SYK主要在乳腺导管上皮细胞的胞质中表达,而在泌乳期SYK在腺泡上皮细胞中表达。结果提示SYK是乳腺上皮细胞增殖与分化的调节因子,主要参与干乳期乳腺组织的重建过程。 相似文献