Trueness-to-type and agronomic characteristics of Coffea arabica trees micropropagated by the embryogenic cell suspension technique

Trueness-to-type and agronomic characteristics of trees of four coffee (Coffea arabica L.) F(1) hybrid clones derived from embryogenic cell suspensions were compared with those of trees produced from in vitro microcuttings. Three types of variants were observed among the 644 trees derived from embryogenic suspensions. Total frequency of the variants was 2.1% for trees originating from embryogenic cell suspensions, whereas no variant was found among the trees produced from microcuttings. The variant known as "thick leaf" had thick leaves, many abnormally starry flowers and low yields of large fruit. The "dwarf" variant was characterized by slow growth and small fruit. The "dwarf peaberry" variant had abnormal seeds in a single cavity, in addition to the "thick leaf" and "dwarf" characteristics. Compared with normal trees, the variants differed in leaf density and number of chloroplasts per guard cell. The variants aside, there were no differences in the main agronomic characteristics between trees produced from embryogenic suspensions and those produced from microcuttings. For all four clones, the trees had vegetative characteristics, productivity, fertility, and bean biochemical, mineral and organoleptic characteristics that were identical to those of the controls. We conclude that it is possible to generate coffee trees commercially with normal agronomic performance from embryogenic suspensions, because the frequency with which somaclonal variants occur is limited.     

小粒咖啡庇荫效应及其间作优势组合的探讨

在云南热区合理的间作能为小粒咖啡提供适度荫蔽,改善生态环境,有利于咖啡生长发育,减轻寒害、病虫和草害影响,为咖啡丰产、稳产,提高园地产出率,降低成本创造条件;橡胶+咖啡,香蕉+咖啡,澳洲坚果+咖啡,龙眼、荔枝+咖啡是小粒咖啡间作的优势组合。     

云南小粒种咖啡荫蔽栽培研究

云南小粒种咖啡荫蔽栽培有利于咖啡营养生长和生殖生长的平衡,能有效控制主要病虫和草害的发生,有利于咖啡丰产、稳产,提高品质和降低生产成本;咖啡与热带经济作物、果树间作,改善了生态环境,提高了土地利用率和单位土地面积的经济效益:是发展云南小粒种咖啡的最好方式。     

缅甸小粒种咖啡生产考察报告

通过国家农业部"走出去"科技合作项目,云南农垦热带农业科技合作代表团应邀对缅甸小粒种咖啡生产进行实地考察,了解缅甸农业机构开展国际合作、咖啡研究及种植生产技术出现快速发展的状况。缅甸种植小粒种咖啡采取高荫蔽度的间作模式,虽然单株产量不高,但是咖啡品质好,已成为精品咖啡,进入以美国为主的国际市场,获得了较好的经济效益。此外,缅甸有较好的咖啡生长的自然条件,土地肥沃、资源丰富,具有今后开展中缅咖啡项目合作的良好基础。     

咖啡种质资源的引进、研究及利用

对引进保存的个咖啡种质进行种质特性和适应性研究及评价;对用部分种质的基因型作有性杂交,164获得份不同杂交组合种子实生苗材料进行筛选;推广小粒咖啡卡蒂莫(15CIFC7963F6)生产种植6 984hm2,取得显著经济效益。     

Effects of water deficit on flower opening in coffee (Coffea arabica L.)

The response of coffee (Coffea arabica L.) floral buds to different water deficits followed by re-irrigation was investigated. Flower opening was stimulated by irrigation after one period of water deficit if predawn leaf water potential declined below -0.8 MPa. Similar stimulation of flowering was observed when less severe but more prolonged water deficits (ca. -0.3 to -0.5 MPa for two weeks) were imposed, even if water deficit was relieved by re-irrigation several times during this period. Consistent results were obtained in the field and in two greenhouse locations. Stimulation of flower opening by water deficit followed by re-irrigation was restricted to buds at the "open white cluster" stage of development (Stage 4). Only buds at this stage exhibited development of secondary xylem. Split-root experiments indicated that a root signal stimulated flower opening, independently of predawn or midday leaf water status. Frequent irrigation to prevent flowering, followed by a controlled water deficit and re-irrigation to stimulate flowering, may represent a practical method to synchronize flowering and shorten the harvest period in leeward coffee production areas in Hawaii.     

云南小粒咖啡出现早衰的原因及防控对策

分析了近年云南小粒咖啡早衰减产问题,认为全球气温变化是一个重要的原因,连年持续干旱、冬季寒害、田间管理松弛以及锈病流行与天牛危害叠加的影响导致了云南咖啡出现早衰;提出了改善咖啡种植环境、加强水利设施建设、选种抗锈品种、防控天牛害虫、加强肥料管理的对策;特别阐述了解决云南咖啡早衰乃至云南咖啡可持续发展问题的一个根本举措,是要引进、选育和推广种植以抗锈为主的高产优质品种的观点。     

Somaclonal variation in growth, leaf phenotype and gas exchange characteristics of poplar: utilization of leaf morphotype analysis as a basis for selection

Populus hybrid TT32 lines produced from 15 treatment tissue culture regimes exhibited somaclonal variation in morphological and gas exchange parameters. Within four years of regeneration, discrete lines showing statistically validated superior, or inferior, growth performance relative to the parental reference clone were identified. Significant differences in the ratio of leaf length/width between treatment lines provided the earliest reliable indicator of the divergence in overall growth performance. Despite discernible variation in leaf phenotype among primary regenerants and secondary propagules within individual lines, the leaf length/width ratio was identified as a potential parameter for predicting growth performance. Its subsequent use led to the recognition of four distinct leaf morphotypes; cordate, ovate, reniform and oval. Two or more of these morphotypes were distributed within each of the original 15 treatment lines. Regrouping the data on the basis of leaf morphotype resulted in a clear segregation of the morphological traits, and revealed differences that were not readily apparent by statistical analysis based on treatment groups. The demonstration of similar relative performances by individual morphotypes with respect to a range of growth and gas exchange parameters confirmed that variation in leaf morphology was indicative of differential photosynthetic performance. Somaclonal variants with a leaf morphology was indicative of differential photosynthetic performance. Somaclonal variants with a leaf morphotype closest to that of the parental line showed the highest overall potential for selection, suggesting that the greatest benefits accrue from a minimal disturbance of the parental leaf phenotype.     

Fruit load and branch ring-barking affect carbon allocation and photosynthesis of leaf and fruit of Coffea arabica in the field

Increasing fruit load (from no berries present to 25, 50 and 100% of the initial fruit load) significantly decreased branch growth on 5-year-old coffee (Coffea arabica L.) trees of the dwarf cultivar 'Costa Rica 95', during their third production cycle. Ring-barking the branches further reduced their growth. Berry dry mass at harvest was significantly reduced by increasing fruit load. Dry matter allocation to berries was four times that allocated to branch growth during the cycle. Branch dieback and berry drop were significantly higher at greater fruit loads. This illustrates the importance of berry sink strength and indicates that there is competition for carbohydrates between berries and shoots and also among berries. Leaf net photosynthesis (P(n)) increased with increasing fruit load. Furthermore, leaves of non-isolated branches bearing full fruit load achieved three times higher P(n) than leaves of isolated (ring-barked) branches without berries, indicating strong relief of leaf P(n) inhibition by carbohydrate demand from berries and other parts of the coffee tree when excess photoassimilates could be exported. Leaf P(n) was significantly higher in the morning than later during the day. This reduction in leaf P(n) is generally attributed to stomatal closure in response to high irradiance, temperature and vapor pressure deficit in the middle of the day; however, it could also be a feedback effect of reserves accumulating during the morning when climatic conditions for leaf P(n) were optimal, because increased leaf mass ratio was observed in leaves of ring-barked branches with low or no fruit loads. Rates of CO(2) emission by berries decreased and calculated photosynthetic rates of berries increased with increasing photosynthetic photon flux (PPF) especially at low PPFs (0 to 100 micromol m(-2) s(-1)). The photosynthetic contribution of berries at the bean-filling stage was estimated to be about 30% of their daily respiration costs and 12% of their total carbon requirements at PPF values commonly experienced in the field (200 to 500 micromol m(-2) s(-1)).     

Evaluation of somatic embryo production during embryogenic tissue proliferation stage using morphology,maternal genotype,proliferation rate and tissue age of Pinus thunbergii ParlOA

To determine the optimal embryogenic capacity(somatic embryo production) of the selected elite nematoderesistant genotypes of Pinus thunbergii,variables such as embryogenic tissue(ET) morphology,maternal genotype,proliferation rate and tissue age were analyzed.ET morphology and histological evaluation of the proliferation stage showed a decrease in filamentous clump and protuberant surfaces and a decline in the acetocarmine-staining area,which indicates a decrease in somatic embryo production(SE...     

Carbohydrates in individual poplar fine roots: effects of root age and defoliation

Late-summer starch accumulation in fine roots of poplars (Populus x canadensis Moench.) defoliated by gypsy moth (Lymantria dispar L.) lagged behind that in fine roots of undefoliated trees. If starch concentration declines with age, defoliation-induced changes in root system age structure could be partly responsible for this difference. To test this hypothesis, we measured fine-root starch and soluble sugar concentrations in roots of known age from trees in defoliated and undefoliated plots. There was a significant interaction between the effects of defoliation and root type (white, brown, or dead) on fine root soluble sugar concentration because of the high concentration of soluble sugars in white roots from trees in undefoliated plots. Both root starch and soluble sugar concentrations were variable among individuals of each age class. The population frequency distributions for starch and soluble sugar concentrations were both right-skewed, and fit by exponential functions. These data are most consistent with direct defoliation effects on a labile and dynamic pool of carbohydrates in poplar fine roots, rather than indirect defoliation effects on root system age structure.     

云南小粒咖啡优良品种比较试验及丰产栽培示范

以德宏热带农业科学研究所咖啡种质库选出的13个小粒咖啡品种为材料,在云南省4个不同的生态类型区建立咖啡优良品种比较试验区和丰产栽培试验区,以验证各品种的生长、产量和品质等优良特性,和在不同生态类型区的稳定性,试验结果筛选出CIFC7963、28号可在云南适宜植区大规模种植;T8667、T5175、CatimorP88、CatimorP128等4个品种可以扩大种植。景洪试验点年均气温高(22.4℃),超过小粒种咖啡对最适年均温(19～21℃)的要求,不适宜种植小粒种咖啡。     

Seasonal,tissue and age influences on frequency and biodiversity of endophytic fungi of Citrus sinensis in Iran

A total of 4,009 endophytic fungal isolates were recovered from healthy leaves, bark and xylem of Citrus sinensis in different seasons and age‐classes. Corresponding to those factors, the majorities of fungal isolates were recovered from leaves, 2‐yr‐old trees and winter, respectively. Fungal isolates were initially categorized based on cultural and morphological characters, and representatives of each morphotype were subjected to molecular identifications based on ITS‐rDNA and β‐tubulin sequences. All isolates obtained in this study belonged to the phyla Ascomycota and Basidiomycota assigned to 30 fungal taxa. The most abundant fungal species were Alternaria spp. Species diversity indices of Margalef richness and Shannon–Wiener revealed a high diversity of fungal taxa recovered from leaf and during winter. Frequency (%) of fungal endophytes was highest in winter followed by summer, autumn, and spring significantly, whereas the corresponding sequence was winter, autumn, summer and spring for the diversity of endophytic fungi. Overall, the results of this study based on the community and diversity of endophytic fungal species in C. sinensis showed that the type of plant tissue, season of sampling and age of tree play a driving role on their abundance while their diversity was mainly dependent on the type of plant tissue, season of sampling than on the age of tree.     

基因型及胚发育对百合远缘杂交胚拯救的影响

以亚洲百合、铁炮百合、东方百合及喇叭百合远缘杂交子代为材料,进行了不同基因型、不同胚状态、不同胚龄的胚拯救试验.结果表明:不同基因型间胚囊(胚)获得率及萌发率均存在较大差异;成熟胚萌发率在85%以上,成熟胚囊萌发率在10%～59%,幼嫩胚囊萌发率很低,大多在20%以下;成熟胚囊、幼嫩胚囊、成熟胚组培苗生长时非正常苗出现情况亦各不相同;所有基因型最适宜的胚拯救胚龄为39～90天,不同基因型随着胚龄的不同其胚囊(胚)获得率呈现不同的变化规律.     

Effects of age and frequency of cutting on productivity of Mediterranean deciduous fodder tree and shrub plantations

Deciduous fodder trees and shrubs are important feed resources for the critical summer period in semi-arid and sub-humid Mediterranean environments. Eleven such species were established in central Macedonia, Greece as 1-year old seedlings in 1987 and studied through 1994. Height and biomass were statistically different among species and tended to increase with the age of plants. Common Robinia pseudoacacia was the tallest and the most productive species, reaching more than 3 m height and 4 kg DM/plant 8 years since planting; it was followed by Ostrya carpinifolia, the variety monophylla of Robinia, Colutea arborescens and Amorpha fruticosa. Grazeable proportion of total biomass was about 50% and decreased as the age of plants increased. Repeated annual cutting resulted in significant reduction of both height and total biomass by 51% and 88%, respectively as compared with uncut plants at the end of the eighth year while frequent cutting tended to increase the grazeable proportion of biomass after the fourth year. To attain an optimum combination of quantity and quality of biomass in deciduous fodder tree and shrub plantations cutting or grazing should not start earlier than the third year since establishment.     

Long-term effects of fire frequency on carbon storage and productivity of boreal forests: a modeling study

Climate change is predicted to shorten the fire interval in boreal forests. Many studies have recorded positive effects of fire on forest growth over a few decades, but few have modeled the long-term effects of the loss of carbon and nitrogen to the atmosphere. We used a process-based, dynamic, forest ecosystem model, which couples the carbon, nitrogen and water cycles, to simulate the effects of fire frequency on coniferous forests in the climate of Prince Albert, Saskatchewan. The model was calibrated to simulate observed forest properties. The model predicted rapid short-term recovery of net primary productivity (NPP) after fire, but in the long term, supported the hypotheses that (1) current NPP and carbon content of boreal forests are lower than they would be without periodic fire, and (2) any increase in fire frequency in the future will tend to lower NPP and carbon storage. Lower long-term NPP and carbon storage were attributable to (1) loss of carbon on combustion, equal to about 20% of NPP over a 100-200 year fire cycle, (2) loss of nitrogen by volatilization in fire, equal to about 3-4 kg N ha(-1) year(-1) over a 100-200 year fire cycle, and (3) the fact that the normal fire cycle is much shorter than the time taken for the forest (especially the soil) to reach an equilibrium carbon and nitrogen content. It was estimated that a shift in fire frequency from 200 to 100 years over 1000 Mha of boreal forest would release an average of about 0.1 Gt C year(-1) over many centuries.     

落羽杉属种源研究∶生长和生物量变异

对落羽杉属不同种源、家系和树种苗期、幼林期生长量（高、胸径、冠幅）、保存率和生物量及其分配（总生物量，干、枝、叶、根生物量及其比值）进行了５年研究。发现落羽杉不同种源、家系间、内均存在显著的性状变异。变异系数以种源、家系间最大，个体间其次。生物量的变异系数大于生长量。未发现不同种源、家系在不同地点存在明显互作。采用多性状综合选择法评选出７个优良种源、家系或树种（１６００、４０１、中山杉、３０４、５０２、鸡公山落羽杉和７０１），它们的生长量、生物量大，叶生物量较大，地上部分生物量／根生物量比值大。其综合选择指数值比平均值大１３．９７％～３５．３３％，树高比平均值大１１．２２％～２４．４９％，比本地近似树种（江宁池杉）大３４．５４％～４６．１８％；胸径比平均值大１２．８１％～４８．２８％，比江宁池杉大３８．７９％～８２．４２％；总生物量比平均值大５４．５９％～１１０．６７％；保存率一般也高于平均值及本省江宁池杉。这些优良种源、家系或树种可以在江苏全省及国内类似地区作用材、防护和观赏树种推广应用。