Neutral detergent fiber concentration in corn silage influences dry matter intake, diet digestibility, and performance of Angus and Holstein steers

Twelve Angus (237 +/- 13 kg) and twelve Holstein (235 +/- 15 kg) steers were used to determine whether corn silage-based diets with different NDF levels influence DMI to a similar extent in Angus and Holstein steers and as body weight of the steers increase. Steers were randomly assigned to individual slatted-floor pens and used in a crossover design consisting of six 14-d periods. Experimental diets contained corn silage from a normal hybrid (low-fiber; LF) and its male-sterile counterpart (high-fiber; HF) and were alternated each period. The LF and HF diets contained 33.8 and 50.8% NDF, respectively. The HF diet decreased (P < 0.01) overall steer mean DMI 14.0% relative to LF, with mean differences increasing as steers increased in BW (P < 0.01). Feeding the HF diet also reduced ADG by an average of 13.8% relative to the LF diet (P < 0.01). Holstein steers consumed 14.4% more DM and gained 14.3% faster (P < 0.01) than Angus steers. There was a fiber level x breed-type interaction (P = 0.08) for efficiency of gain. Angus steers receiving the HF diet had greater efficiency of gain than Angus steers consuming the LF diet; however, Holstein steers consuming the LF diet had greater efficiency of gain than those receiving the HF diet. The HF treatment reduced total-tract digestibility of DM and GE by 4.6 and 4.5%, respectively (P < 0.01), and decreased DE intake by 20.5% (P < 0.01) but increased apparent totaltract digestibility of NDF and ADF (9.4 and 8.4%, respectively; P < 0.01). Holstein steers had similar digestibility of DM and GE (P > 0.10) but had greater DE intake (P < 0.01) compared to Angus steers. There were fiber level x breed-type interactions for total-tract digestibility of NDF and ADF (P < 0.06). The difference in DM digestibility was negatively associated with the difference in DMI (r2 = 0.23; P < 0.01) for LF minus HF within Angus steers, but not within Holstein steers (P = 0.42). Total-tract digestibility of NDF and ADF was 4.1 and 3.4% lower for the HF diet but was only 1.1 and 0.6% lower for the LF diet when fed to Holstein compared to Angus steers. Results from this trial demonstrate that high-NDF corn silage-based diets reduced intake of both Angus and Holstein steers, and this reduction in DMI continued as steers increased in BW from 235 to 330 kg. Breed differences were also noted for digestible energy intake as influenced by fiber level.     

Effects of feeding growing cattle high-concentrate diets at a restricted intake on feedlot performance

Three trials were conducted to compare effects of restricted intake of high-concentrate diets vs ad libitum intake of corn silage diets during the growing phase on feedlot cattle performance. In Trial 1, 120 steers (initial BW, 246 kg) were fed 1) a corn silage-based diet ad libitum, 2) a high-moisture corn-corn silage-based diet with intake restricted to a level 20% less than that of the corn silage diet or 3) a high-moisture corn-based diet with intake restricted to a level 30% less than that of the corn silage diet. Steers fed the 20% restricted corn-corn silage-based diet tended (P = .07) to gain slower than those fed the corn silage or 30% restricted high-concentrate diet. Feed efficiency and diet digestibility were greatest for steers fed the 30% restricted-intake, high-concentrate diet (P less than .01). Performance of steers during the subsequent 118-d finishing period was not affected (P greater than .65) by source of energy during the growing period. In Trial 2, ADG of steers fed the 30% intake-restricted, high-concentrate diet was lower (P less than .01) than that of steers with ad libitum access to corn silage. During the 84-d growing period, steers fed supplemental blood meal had 8.3% greater gains and a 6% greater efficiency of feed use than those fed supplemental soybean meal (P less than .01). Monensin did not affect (P = .82) performance of steers fed 30% restricted-intake diets. During the 76-d finishing period, gains and feed conversion were improved (P less than .01) for steers fed the restricted-intake diet in the growing period compared with those given ad libitum access to corn silage. During the growing period in Trial 3, ADG of steers restricted-fed an all-concentrate diet were slightly greater (P less than .10) than ADG of those given ad libitum access to corn silage. Gains did not differ (P = .37) during the subsequent finishing period when steers were switched to 85 or 100% concentrate diets. We concluded that intake of all concentrate diets can be restricted to achieve gains equal to those of steers given ad libitum access to corn silage-based diets without detrimental effects on finishing performance.     

Effects of supplemental energy and/or degradable intake protein on performance,grazing behavior,intake, digestibility,and fecal and blood indices by beef steers grazed on dormant native tallgrass prairie

To evaluate the effects of balancing total diet degradable intake protein with dietary total digestible nutrients (TDN), we conducted two studies during 2 yr with 100 (302 +/- 8 kg initial BW) mixed-breed yearling steers and 12 ruminally cannulated steers (526 +/- 28 kg). Steers individually received one of four supplements 5 d/wk while grazing dormant native tallgrass prairie. Supplements included: 1) corn and soybean meal, balanced for total diet degradable intake protein in relation to total diet TDN (CRSBM), 2) corn and soybean hulls, equal in supplemental TDN to CRSBM (CORN), 3) soybean meal, equal in supplemental degradable intake protein to CRSBM (SBM), or 4) a cottonseed hull-based control supplement (CONT). At each feeding (5 d/wk), steers consumed 13.6, 13.6, or 4.2 g of dry matter/kg of body weight, or 178 g of DM, respectively, of supplement. Steers fed CRSBM had greater (P < 0.01) average daily gain than cattle fed CORN or SBM. Feeding soybean meal (CRSBM, SBM) resulted in improved (P < 0.01) efficiency of supplement. Grazing time, intensity, and harvesting efficiency were reduced (P < 0.05) by corn supplementation (CRSBM and CORN), whereas the number of grazing bouts per day was increased (P < 0.08). Intake and digestibility of forage organic matter were reduced (P < 0.01) for steers supplemented with corn (CORN and CRSBM) vs cattle not fed corn (SBM and CONT). Total diet digestibility (P < 0.12) and digestible organic matter intake (P < 0.01) were greater for CRSBM-fed steers than for cattle fed either CORN or SBM. Steers fed CRSBM had greater (P < 0.01) fecal nitrogen and serum insulin than cattle fed CORN or SBM. Corn-fed cattle had lesser (P < 0.01) fecal pH and ADF concentrations than steers not consuming grain. Cattle fed supplements with soybean meal (CRSBM and SBM) had greater (P < 0.01) serum urea nitrogen than steers fed supplements without soybean meal (CORN, CONT). Supplemented steers grazing dormant tallgrass prairie had a greater rate of gain, with the greatest response in animal performance occurring when grain supplements were balanced for total diet degradable intake protein in relation to total diet TDN. These results lead us to suggest that grain-supplemented cattle grazing dormant tallgrass prairie require a balance of total diet degradable intake protein in relation to total diet TDN to optimize animal performance.     

Brown midrib-3 corn silage improves digestion but not performance of growing beef steers

The brown midrib-3 (bm3) gene mutation has been incorporated into corn plants to potentially improve fiber digestibility. The objectives of this study were to determine the effect of bm3 corn silage on digestion and performance of growing beef steers and to determine whether limiting intake would further enhance fiber digestibility of bm3 corn silage. A bm3 hybrid and its isogeneic normal counterpart were harvested at three-quarters kernel milk line. Neutral detergent fiber, ADF, and ADL were 4.5, 6.9, and 1.9 units lower, respectively, and DM was 5.4 units higher for bm3 than for normal silage. In Trial 1, eight ruminally fistulated Angus crossbred steers (224 +/- 24 kg) were randomly assigned to a 2 x 2 factorial arrangement of treatments in a replicated 4 x 4 Latin square design. Steers had ad libitum feed access or were restricted to 80% of ad libitum intake of diets containing 86% normal corn silage (Control) or bm3 corn silage (BMCS). The remainder of the diets consisted of soybean meal, urea, monensin, vitamins, and minerals. Dry matter intake was greater (P < 0.01) for steers offered ad libitum access to BMCS than for those with ad libitum access to the Control diet. The BMCS treatment resulted in improved (P < 0.05) apparent total-tract digestibility of DM, OM, NDF, and ADF. Mean concentration of total VFA and molar proportions of acetate were increased (P < 0.05) by feeding BMCS. There tended to be a DMI x hybrid interaction (P = 0.16) for apparent total-tract digestibility of NDF. When diets were offered ad libitum, BMCS increased NDF digestibility by 10.5 percentage units compared with Control, but, when DMI was limited, BMCS increased NDF digestibility by 15.8 percentage units. In Trial 2, 128 steer contemporaries of those used in Trial 1 (245 +/- 13 kg) were offered ad libitum access to BMCS or Control diets as used in Trial 1. After a 112-d treatment period, concentrate in the diet was increased, and all steers were fed a common finishing diet. During the 112-d treatment period, steers receiving BMCS consumed 0.45 kg more DM/d (P < 0.05) and had similar ADG (P > 0.10), compared with those steers receiving the Control silage. This resulted in poorer (P < 0.01) feed efficiency for steers receiving BMCS. Finishing phase and overall performance of the steers was not different (P > 0.10) due to treatment. Although feeding BMCS in growth-phase diets resulted in increased daily DMI and improved digestibility of DM and fiber, it did not result in improved steer feedlot ADG compared with Control silage.     

Comparison of wheat or corn dried distillers grains with solubles on rumen fermentation and nutrient digestibility by feedlot heifers

A 5 × 5 Latin square design trial was conducted to evaluate rumen fermentation and apparent nutrient digestibility in 5 rumen-cannulated heifers (420 ± 6 kg) fed a barley-based finishing diet supplemented with 20 or 40% wheat or corn dried distillers grains with solubles (DDGS). The composition of the control diet was 88.7% rolled barley grain, 5.5% supplement, and 5.8% barley silage (DM basis). Increasing the quantity of corn DDGS in the ration resulted in a quadratic decrease in DMI (P = 0.04) and OM intake (P = 0.05). Rumen pH, pH duration, and area under rumen pH thresholds of 5.8 or 5.5 were not affected (P > 0.05) by treatment. Inclusion of wheat DDGS resulted in a quadratic increase (P = 0.05) in pH area below the cutoff value of 5.2, with the most pronounced effect at 20% inclusion. Wheat DDGS linearly increased (P = 0.01) rumen NH(3)-N concentrations. Increasing the inclusion rate of wheat and corn DDGS resulted in quadratic (P = 0.05) and linear (P = 0.04) decreases in rumen propionate, whereas butyrate increased quadratically (P < 0.01) and linearly (P < 0.01), respectively. Feeding wheat DDGS linearly decreased (P < 0.01) DM and OM digestibility values. Inclusion of corn DDGS increased the digestibility values of ether extract (P = 0.05; quadratic response) and CP (P < 0.01; linear response). Neutral detergent fiber digestibility increased in a linear fashion (P = 0.01) as both wheat and corn DDGS inclusion increased, whereas ADF digestibility increased linearly (P = 0.03) for wheat and quadratically (P = 0.02) for corn DDGS. Increased inclusion of wheat DDGS resulted in a linear decrease in GE digestibility (P = 0.01), whereas increasing corn DDGS inclusion linearly increased (P < 0.01) the DE content of the diet. Feeding both wheat and corn DDGS linearly increased (P = 0.01) the excretion of N and P. In summary, replacement of barley grain with up to 40% wheat or corn DDGS did not mitigate rumen pH conditions associated with mild to moderate acidosis in heifers fed a barley-based finishing diet. Supplementing corn DDGS increased nutrient digestibility of all nutrients and, as a result, led to greater DE content. Supplementation of wheat DDGS reduced DM and OM digestibility values, with no effect on DE content. Increased N and P excretion by heifers fed DDGS at 20 or 40% of dietary DM presents a challenge for cattle feeders with respect to nutrient management.     

Effects of feeding high-oil corn to beef steers on carcass characteristics and meat quality

To assess the effects of feeding high-oil corn on carcass characteristics and meat quality, 60 yearling steers were fed high concentrate diets containing either control corn (82% of diet), high-oil corn (82% of diet), or high-oil corn at a concentration that was isocaloric with the control diet (74% of diet). After being fed for 84 d, steers were slaughtered. At 72 h postmortem, carcass data were collected and rib sections from five steers grading U.S. Choice and five steers grading U.S. Select from each treatment were collected, vacuum packaged, and aged for 14 d. Three steaks (2.54 cm thick) were removed from each rib for Warner-Bratzler shear force measurement, sensory appraisal, and fatty acid composition analyses. Data were analyzed with treatment as the main effect for the carcass data and treatment, quality grade, and two-way interaction in the model for the longissimus data. The two-way interaction was nonsignificant (P > 0.05) for all variables tested. No differences were detected (P > 0.05) in carcass measurements except for marbling scores and quality grades, both of which were greater (P < 0.05) for carcasses from steers fed the high-oil corn. Overall, 78% of steers fed the high-oil corn graded U.S. Choice compared with 47% for the control and 67% for isocaloric group. Shear force and sensory properties of the longissimus were not different (P > 0.05) among treatments. Steaks from U.S. Choice carcasses rated higher (P < 0.05) for tenderness and tended to rate higher (P < 0.10) for juiciness. Feeding the isocaloric and high-oil diets increased (P < 0.05) linoleic acid, arachidonic acid, and the total PUFA content of lipid extracted from the longissimus. Saturated fatty acid percentage was lowest (P < 0.05) for high-oil corn and highest (P < 0.05) for control, with isocaloric being intermediate. Feeding high-oil corn increased (P < 0.05) pentadecyclic acid, margaric acid, and total odd-chain fatty acid content. Feeding high-oil corn in finishing beef cattle diets enhanced intramuscular lipid deposition and increased unsaturation of fatty acids of the longissimus.     

Effects of wet corn gluten feed and roughage levels on performance, carcass characteristics, and feeding behavior of feedlot cattle

Two experiments were conducted to evaluate the effects of feeding different levels of wet corn gluten feed (WCGF) and dietary roughage on performance, carcass characteristics, and feeding behavior of feedlot cattle fed diets based on steam-flaked corn (SFC). In Exp. 1, crossbred steers (n = 200; BW = 314 kg) were fed 4 dietary treatments (DM basis): a standard SFC-based diet containing 9% roughage (CON) and 3 SFC-based diets containing 40% WCGF, with either 9, 4.5, or 0% roughage. A linear (P = 0.04) increase in final BW and DMI (P < 0.01) was observed in diets containing WCGF as dietary roughage increased. Steers fed WCGF and higher levels of roughage had greater (P = 0.01) ADG than steers fed lower levels of roughage. Steers fed the CON diet had lower (P = 0.04) daily DMI and greater (P = 0.03) G:F than those fed WCGF. Most carcass characteristics of steers fed CON did not differ (P > 0.10) from those of steers fed WCGF. Based on feed disappearance and visual scan data, consumption rate did not differ (P > 0.10) among treatments; however, feeding intensity (animals present at the bunk after feeding) was greater for steers fed CON (P < 0.01) than for steers fed WCGF. In Exp. 2, yearling crossbred steers (n = 1,983; BW = 339 kg) were fed 4 dietary treatments (DM basis): a standard SFC-based control diet that contained 9% roughage (CON) and 3 SFC-based diets containing either 20% WCGF and 9% roughage or 40% WCGF with 9 or 4.5% roughage. Steers fed the CON diet tended to have lower final BW (P = 0.14), ADG (P = 0.01), and DMI (P < 0.01) than steers fed diets containing WCGF. Steers fed the 20% WCGF diet had greater (P = 0.08) G:F than steers fed the 40% WCGF diets. With 40% WCGF, increasing roughage from 4.5 to 9% decreased (P < 0.01) G:F and increased (P = 0.06) DMI. Gain efficiency was improved (P < 0.01) for steers fed CON vs. those fed diets containing WCGF, whereas HCW (P = 0.02) and dressing percentage (P < 0.01) were greater for steers fed WCGF. Percentage of cattle grading USDA Choice was greater (P = 0.02) for cattle fed WCGF. Results suggest that replacing SFC with up to 40% WCGF increased ADG and decreased G:F when 4.5 to 9.0% roughage was supplied. More CON steers were present at the feed bunk during the first hour after feeding than WCGF steers, suggesting that including WCGF at 40% of the diet affected feeding behavior.     

Effects of supplemental fat source on nutrient digestion and ruminal fermentation in steers

Five Holstein steers (235 kg of BW) fitted with ruminal, duodenal, and ileal cannulas were used in a 5 x 5 Latin square design experiment to determine the effects of supplemental fat source on site and extent of nutrient digestion and ruminal fermentation. Treatments were diets based on steam-flaked corn containing no supplemental fat (control) or 4% (DM basis) supplemental fat as tallow, dried full-fat corn germ (corn germ), corn oil, or flax oil. Fat supplementation decreased (P < 0.08) ruminal starch digestion but increased (P < 0.03) small intestinal starch digestion as a percentage of intake. Feeding corn germ decreased (P < 0.09) ruminal starch digestion and increased (P < 0.03) large intestinal starch digestion compared with steers fed corn oil. Large intestinal starch digestion was less (P < 0.04), and ruminal NDF digestion was greater (P < 0.09) for steers fed tallow compared with steers fed other fat sources. Small intestinal (P < 0.08) and total tract NDF digestibilities were greater (P < 0.02) for steers fed corn germ than for those fed corn oil. Feeding tallow increased total ruminal VFA (P < 0.03) and NH(3) (P < 0.07) concentrations compared with steers fed the other fat sources. Feeding corn germ led to a greater (P < 0.02) rate of ruminal liquid outflow compared with corn oil. A diet x hour interaction (P < 0.04) occurred for ruminal pH, with steers fed corn oil having the greatest ruminal pH 18 h after feeding, without differences at other time points. Fat supplementation increased (P < 0.09) ruminal concentrations of Fusobacterium necrophorum. Duodenal flow of C18:3n-3 was greater (P < 0.01) for steers fed flax oil compared with those fed corn oil. Feeding corn germ led to less (P < 0.01) ruminal biohydrogenation of fatty acids compared with corn oil. Steers fed tallow had greater small intestinal digestibility of C14:0 (P < 0.02) and C16:1 (P < 0.04) than steers fed the other fat sources. Fat supplementation decreased (P < 0.06) small intestinal digestibility of C18:0. Feeding corn germ decreased (P < 0.10) small intestinal digestibility of C18:1 compared with corn oil. It appears that source of supplemental fat can affect the site and extent of fatty acid and nutrient digestion in steers fed diets based on steam-flaked corn.     

Effect of hybrid, maturity, and mechanical processing of corn silage on intake and digestibility by beef cattle

A study involving a 2 x 2 x 2 factorial arrangement of treatments was conducted to evaluate effects of hybrid (Pioneer 3335 and 3489), maturity (half milkline and blacklayer), and mechanical processing (field chopper with and without on-board rollers engaged) on intake and digestibility of corn silage. Forty Angus steers (322 +/- 5.2 kg BW) were assigned to the eight silage treatments (five steers per treatment) and individually fed using electronic gates. Diets consisted of 60% corn silage and 40% chopped alfalfa hay (DM basis). Following a 5-d adaptation period, intake was measured for 7 d and subsequently fecal samples were collected for 5 d. Chromic oxide (5 g/d) was fed beginning 7 d before fecal sample collection and digestibility was determined by the ratio of Cr in the feed and feces. Steers were reallocated to treatments and these procedures were repeated, providing 10 observations per treatment. In addition, all silages were ruminally incubated in six mature cows for 0, 8, 16, 24, 48, and 96 h to determine extent and rate of DM, starch, NDF, and ADF disappearance. Processing increased DMI of hybrid 3489 but did not affect DMI of hybrid 3335 (hybrid x processing; P < 0.06). Total tract digestibility of DM, starch, NDF, and ADF decreased (P < 0.01) as plant maturity increased. Maturity tended to decrease starch digestibility more for hybrid 3489 than for hybrid 3335 (hybrid x maturity; P < 0.10). Processing increased (P < 0.01) starch digestibility but decreased (P < 0.01) NDF and ADF digestibility, resulting in no processing effect on DM digestibility. There was a numerical trend for processing to increase starch digestibility more for latethan for early-maturity corn silage (maturity x processing; P = 0.11). Processing increased in situ rates of DM and starch disappearance and maturity decreased in situ disappearance rates of starch and fiber. These data indicate that hybrid, maturity, and processing all affect corn silage digestibility. Mechanical processing of corn silage increased starch digestibility, which may have been associated with the observed decreased fiber digestibility.     

Corn oil or corn grain supplementation to steers grazing endophyte-free tall fescue. I. Effects on in vivo digestibility, performance, and carcass quality

Twenty-eight Angus (289 +/- 3.8 kg) steers were used in a completely randomized design to evaluate the effect of isocaloric supplementation of 2 different energy sources to steers rotationally grazing tall fescue pastures for 197 d in comparison to positive and negative controls. Steers were supplemented with either corn grain (0.52% BW on a DM basis; PC) or soybean hulls plus corn oil (0.45% BW on a DM basis + 0.10% BW on an as-fed basis; PO) using Calan gates for individual intake measurement. Negative, pasture only (PA), and positive, high-concentrate control diets (85% concentrate:15% roughage on DM basis; C) were also included in the study. Steers on PC, PO, and PA treatments were managed together under a rotational grazing system, whereas C steers were fed a high-concentrate diet for the final 113 d using Calan gates. Forage DMI and apparent DM and NDF digestibility for the grazing treatments were evaluated using Cr(2)O(5) and indigestible NDF as digesta markers. Energy supplementation decreased (P = 0.02) forage DMI (% of BW) with respect to PA, but not (P = 0.58) total DMI. There were no differences (P = 0.53) among grazing treatments on apparent total DM digestibility. However, NDF digestibility was less (P < or = 0.05) in PC than in PO and PA; the latter 2 treatments did not differ (P > 0.05). Overall ADG was greater (P < 0.01) in supplemented, regardless of type, than in nonsupplemented grazing treatments. During the final 113 d, ADG was greater (P < 0.01) in C than in the grazing treatments. Overall supplement conversion did not differ (P = 0.73) between supplement types and was less (P = 0.006) than C. Carcass traits did not differ (P > 0.05) between energy sources. Dressing percentage and HCW were greater (P < 0.01) in supplemented cattle than in PA. Fat thickness and KPH percentage for PA were less (P < 0.05) than for PO but did not differ (P > 0.14) from PC. Marbling score, LM area, and quality grade did not differ (P > 0.05) between grazing treatments. Hot carcass weight for C was heavier (P < 0.001) than for pastured cattle. Quality and yield grades of C carcasses were also greater (P < 0.001) than carcasses from pastured steers. Energy supplementation, regardless of source, to grazing steers increased ADG, dressing percentage, and carcass weight compared with PA steers; however, supplemented steers had less ADG, efficiency, dressing percentage, and carcass weight compared with high-concentrate finished steers.     

Comparison of the effects of three methods of harvesting and storing alfalfa on nutrient digestibility by lambs and feedlot performance of steers

Two lamb digestion and three steer growth experiments were conducted to study the feeding value of alfalfa harvested as direct-cut silage (DCS) with grain added prior to ensiling or as low-moisture silage (LMS) or hay with grain added at feeding. In all experiments, alfalfa-grain mixtures contained approximately 50% alfalfa and 50% concentrate (dry matter [DM] basis). In Exp. 1, lambs fed DCS alone consumed less DM than lambs fed LMS or hay alone or any of the alfalfa-grain mixtures. Apparent digestibilities of DM and fiber components were higher (P less than .05) for DCS than for LMS or hay. Lambs that were fed LMS digested more (P less than .05) DM and fiber components than lambs fed hay. Addition of grain resulted in increased (P less than .05) DM digestibility and decreased (P less than .05) digestibilities of neutral detergent fiber and acid detergent fiber. In Exp. 2, growing steers (271 kg) fed DCS-grain had increased (P less than .05) weight gains compared with steers fed hay-grain. Steers fed any of the alfalfa-grain mixtures gained weight more rapidly (P less than .05) than steers fed corn silage (CS)-based diets. In a third experiment, finishing steers (283 kg) fed DCS-grain, LMS-grain, hay-grain or CS-based diets performed similarly (P greater than .05), although steers fed DCS-grain had higher (P less than .05) dressing percentages and yield grades than steers that were fed the other three diets and were fatter (P less than .05) than those fed LMS-grain or CS. In Exp. 4, lambs fed DCS-grain or LMS-grain had higher (P less than .05) apparent DM and organic matter digestibilities than lambs fed CS-based diets with similar forage:grain proportions. In Exp. 5, finishing steers (326 kg) fed DCS-grain gained similarly (P greater than .05) to steers fed LMS-grain or an 85% concentrate diet based on high-moisture corn. Steers fed CS diets had lower (P less than .05) gains and increased (P less than .05) feed per gain compared with steers fed DCS-grain, LMS-grain or high-moisture corn.     

Influence of genotype and diet on steer performance, manure odor, and carriage of pathogenic and other fecal bacteria. I. Animal performance

Although Brahman crosses constitute a large portion of US beef cattle, little information is available on their response to diverse feed resources compared with Bos taurus steers. Thus, the objectives were to evaluate genotype and diet effects on steer performance during the growing period and subsequent response to a high grain diet during the finishing period. Fifty-one steers [0 (15), 1/4 (20), 1/2 (7), and 3/4 Brahman (9), with the remaining proportion being MARC III] were allotted to 8 pens. Beginning on December 2, steers were individually fed chopped bromegrass hay (n = 26; DM = 85%, CP = 9.5%, ME = 2.19 Mcal/kg) or a corn silage-based diet (n = 25; DM = 51%, CP = 11.9%, ME = 2.75 Mcal/kg) for 119 d. All steers were then fed a high corn diet (DM = 79%, CP = 11.7%, ME = 3.08 Mcal/kg) to a target BW of 560 kg (176 d). Data were analyzed by ANOVA, with genotype, growing diet, and the 2-way interaction included. The interaction was not significant (P > 0.25). The MARC III and 1/2 Brahman steers weighed more (P < 0.01) than 1/4 or 3/4 Brahman steers initially and at the end of the growing period. Weight of bromegrass-fed (325 kg) steers was less than that of corn silage-fed (384 kg) steers at the end of the growing period. Steer ADG and intake of DM, CP, and ME were less (P = 0.087 to 0.001) for 1/4 and 3/4 Brahman than for 0 or 1/2 Brahman steers during growing, finishing, and total, but efficiency of gain did not differ (P > 0.10). Carcass weight, marbling score, quality grade (P < 0.05), and kidney fat (P = 0.06) differed among genotypes. Daily DMI (6.91 vs. 7.06 kg) was similar, but CP (0.66 vs. 0.84 kg) and ME (15.2 vs. 19.4 Mcal) intake of bromegrass fed was less (P = 0.001) than those of corn silage-fed steers. Values for DMI/gain (22.3 vs. 7.43 kg/kg), CP intake/gain (2.13 vs. 0.88 kg/kg), and ME intake/gain (48.8 vs. 20.4 Mcal/kg) were greater (P < 0.001) in bromegrass-fed than corn silage-fed steers. Over the total study, ADG was lower (0.96 vs. 1.01 kg), and DMI (7.82 vs. 7.19 kg), DMI/gain (8.21 vs. 7.10 kg/kg), and ME intake/gain (22.6 vs. 20.9 Mcal/kg) were greater (P < 0.05) in bromegrass-fed than in corn silage-fed steers. Carcass weight, dressing percent, adjusted backfat, and yield grade (P < 0.05) were greater for corn silage-fed than for bromegrass-fed steers. Feed intake and performance, but not efficiency, differed among these genotypes. Compensatory performance during finishing was insufficient to overcome reduced performance during the growing period.     

Effects of distillers grains with high sulfur concentration on ruminal fermentation and digestibility of finishing diets

Twelve ruminally cannulated crossbred Angus steers were used to evaluate ruminal fermentation characteristics and diet digestibility when 30% (DM) corn dried distillers grains with solubles (DDGS) containing 0.42 or 0.65% (DM) of dietary S was incorporated into finishing diets based on steam-flaked corn (SFC) or dry-rolled corn (DRC). The study was a replicated, balanced randomized incomplete block design with a 2 × 2 factorial arrangement of treatments. Factors consisted of dietary S concentration (0.42 and 0.65% of DM; 0.42S and 0.65S, respectively) and grain processing method (SFC or DRC). The 0.65S concentration was achieved by adding H(2)SO(4) to DDGS before mixing rations. Steers were assigned randomly to diets and individual, slatted-floor pens, and fed once daily for ad libitum intake. Two 15-d experimental periods were used, each consisting of a 12-d diet adaptation phase and a 3-d sample collection phase. Samples were collected at 2-h intervals postfeeding during the collection phase. Ruminal pH was measured immediately after sampling, and concentrations of ruminal ammonia and VFA were determined. Fecal samples were composited by steer within period and used to determine apparent total tract digestibilities of DM, OM, NDF, CP, starch, and ether extract. Feeding 0.65S tended (P = 0.08) to decrease DMI but resulted in greater apparent total tract digestibilities of DM (P = 0.04) and ether extract (P = 0.03). Ruminal pH increased (P < 0.05) in steers fed 0.65S diets, which may be attributable, in part, to decreased (P = 0.05) VFA concentrations and greater (P < 0.01) ruminal ammonia concentrations when 0.65S was fed, compared with feeding 0.42S. These effects were more exaggerated in steers fed DRC (interaction, P < 0.01), compared with steers fed SFC. Steers fed DRC-0.65S had greater (P < 0.01) acetate concentration than steers fed DRC-0.42S, but acetate concentration was not affected by S concentration when SFC was fed. Propionate concentration was decreased (P < 0.01) in steers fed SFC-0.65S compared with steers fed SFC-0.42S, but dietary S concentration had no effect on propionate concentration when DRC was fed. Butyrate concentration was less (P < 0.01) in steers fed 0.65S diets than in steers fed 0.42S. Lactate concentrations tended (P = 0.06) to decrease in steers fed 0.65S diets. Feeding DDGS with increased S concentration may decrease feed intake and ruminal VFA concentration but increase ruminal ammonia concentration.     

Effect of cattle age, forage level, and corn processing on diet digestibility and feedlot performance

Three experiments were conducted to determine the effects of cattle age and dietary forage level on the utilization of corn fed whole or ground to feedlot cattle. In Exp. 1, 16 steers were used to investigate the effects of cattle age and corn processing on diet digestibility. Two cattle age categories were evaluated (weanling [254 +/- 20 kg BW] and yearling [477 +/- 29 kg BW]; eight steers per group), and corn was fed either ground or whole to each cattle age category. Cattle age and corn processing did not affect (P > 0.10) diet digestibility of DM, OM, starch, CP, NDF or ADF, and no interactions (P > 0.10) between these two factors were detected. In Exp. 2, the effects of forage level and corn processing on feedlot performance and carcass characteristics were evaluated. One hundred eighty steers (310 +/- 40 kg BW) were allotted to 24 pens, and were fed one of the following diets: high-forage (18.2% corn silage) cracked corn (HFCC); high-forage shifting corn (whole corn for the first half of the trial, then cracked corn until harvest; HFSC); high-forage whole corn (HFWC); low-forage (5.2% corn silage) cracked corn (LFCC); low-forage shifting corn (LFSC); and low-forage whole corn (LFWC). For the high-forage diets, steers fed cracked corn had 7% greater DMI than those fed whole corn, whereas for the low-forage diets, grain processing did not affect DMI (interaction; P = 0.02). No interactions (P > 0.10) between forage level and corn processing were found for ADG and G:F. Total trial ADG and G:F, and percentage of carcasses grading USDA Choice, and carcass yield grade were not affected (P > 0.10) by corn processing. Cattle with fewer days on feed grew faster and more efficiently when cracked corn was fed, whereas cattle with longer days on feed had greater ADG and G:F when corn was fed whole (interaction; P < 0.10). In Exp. 3, the effects of forage level and corn processing on diet digestibility were evaluated. The high-forage cracked corn, high-forage whole corn, low-forage cracked corn, and low-forage whole corn diets used in Exp. 2 were fed to 16 steers (350 +/- 27 kg BW) in a digestion trial. No interactions (P > 0.10) between forage level and corn processing were detected for starch digestibility. Forage level and corn processing (grinding) did not affect (P > 0.10) diet DM, OM, starch, CP, and NDF digestibility. Processing corn did not provide additional benefits to feedlot cattle performance under these experimental conditions.     

Effects of concentrated separator by-product (desugared molasses) on intake, site of digestion, microbial efficiency, and nitrogen balance in ruminants fed forage-based diets

In Exp. 1, 4 ruminally and duodenally cannulated beef steers (444.0 +/- 9.8 kg) were used in a 4 x 4 Latin square with a 2 x 2 factorial treatment arrangement to evaluate the effects of forage type (alfalfa or corn stover) and concentrated separator byproduct (CSB) supplementation (0 or 10% of dietary DM) on intake, site of digestion, and microbial efficiency. In Exp. 2, 5 wethers (44 +/- 1.5 kg) were used in a 5 x 5 Latin square to evaluate the effects of CSB on intake, digestion, and N balance. Treatments were 0, 10, and 20% CSB (DM basis) mixed with forage; 10% CSB offered separately from the forage; and a urea control, in which urea was added to the forage at equal N compared with the 10% CSB treatment. In Exp. 1, intakes of OM and N (g/kg of BW) were greater (P < 0.01) for steers fed alfalfa compared with corn stover. Steers fed 10% CSB had greater (P < 0.08) OM and N intakes (g/kg of BW) compared with 0% CSB-fed steers. Total duodenal, microbial, and nonmicrobial flows of OM and N were greater (P < 0.01) for steers fed alfalfa compared with corn stover. Steers fed 10% CSB had increased (P = 0.02) duodenal microbial flow (N and OM) compared with 0% CSB-fed steers. Forage x CSB interactions (P < 0.01) existed for total tract N digestibility; alfalfa with or without CSB was similar (67.4 vs. 69.5), whereas corn stover with CSB was greater than corn stover without CSB (31.9 vs. -23.9%). True ruminal OM digestion was greater (P < 0.09) in steers fed alfalfa vs. corn stover (73.0 vs. 63.1%) and in steers fed 10 vs. 0% CSB (70.3 vs. 65.8%). Microbial efficiency was unaffected (P > 0.25) by forage type or CSB supplementation. In Exp. 2, forage and total intake increased (linear; P < 0.01) as CSB increased and were greater (P < 0.04) in 10% CSB mixed with forage compared with 10% CSB fed separately. Feeding 10% CSB separately resulted in similar DM and OM apparent total tract digestibility compared with 10% CSB fed mixed. Increasing CSB led to an increase (linear; P < 0.02) in DM, OM, apparent N digestion, and water intake. Nitrogen balance (g and percentage of N intake) increased (linear; P < 0.08) with CSB addition. Feeding 10% CSB separately resulted in greater (P < 0.01) N balance compared with 10% CSB fed mixed. Using urea resulted in similar (P = 0.30) N balance compared with 10% CSB fed mixed. Inclusion of CSB improves intake, digestion, and increases microbial N production in ruminants fed forage-based diets.     

Proportion of corn silage in diets of feedlot steers fed to achieve stepwise increases in growth.

Two experiments were conducted to investigate the effects of proportion of dietary corn silage during periods of feed restriction on performance of steers. In Exp. 1, Simmental x Angus steer calves (n = 107; initial BW = 273 +/- 3.8 kg) were allotted to 12 pens with eight or nine steers/pen and four pens/treatment. Periods of growth were 273 to 366 kg BW (Period 1), 367 to 501 kg BW (Period 2), and 502 to 564 kg BW (Period 3). In two of the dietary regimens, steers were given ad libitum access to feed throughout the experiment and were fed either a 15% corn silage diet in each period or an 85, 50, and 15% corn silage diet in Periods 1, 2, and 3; respectively. In the third feeding regimen, a programmed intake feeding regimen was used. Steers were fed a 15% corn silage diet in each period. However, feed intake was restricted to achieve a predicted gain of 1.13 kg/d in Period 1 and 1.36 kg/d in Period 2, and feed was offered for ad libitum consumption in Period 3. For the entire experiment, ADG was similar (P = 0.41) among treatments and feed efficiency was lower (P < 0.10) for steers in the corn silage regimen than for steers in the programmed intake and ad libitum regimens. In Exp. 2, Simmental x Angus steer calves (n = 106; initial BW = 233 +/- 2 kg) were allotted by BW to 12 pens (three pens/treatment) and fed in three periods similar to those described in Exp. 1. Four feeding regimens were investigated: 1) AL; steers were offered a 15% corn silage diet for ad libitum consumption in all three periods; 2) PI; DMI was programmed to achieve gains as described in Exp. 1; 3) CS-HLL; programmed intake as described above except diets contained 85, 15, and 15% corn silage in Periods 1, 2, and 3, respectively; and 4) CS-HIL; same feeding regimens as CS-HLL, except diets contained 85, 50, and 15% corn silage in Periods 1, 2, and 3, respectively. Steers were given ad libitum access to feed in Period 3. Overall ADG was lower (P < 0.05) for steers in the CS-HLL and CS-HIL feeding regimens than for steers in the AL and PI regimens; feed efficiency was greatest for steers in the PI regimen. Few effects of feeding regimen on carcass characteristics were observed.     

Efficacy of plastic pot scrubbers as a replacement for roughage in high-concentrate cattle diets.

Effects of plastic pot scrubbers on feedlot performance and ruminal metabolism of steers fed all-concentrate diets were determined. In Trial 1, 31 crossbred steers (means initial BW, 290 kg) were penned and fed individually. Treatments were 1) 85% concentrate-15% corn silage diet, 2) 100% concentrate diet, and 3) 100% concentrate diet + ruminal insertion of eight plastic pot scrubbers per steer. During the first 112 d of the trial, steer ADG did not differ (P greater than .10) due to treatment. From d 113 to 152, steers provided with pot scrubbers had 16% greater ADG than those fed the 100% concentrate diet without pot scrubbers (P = .18). In Trial 2, 78 crossbred steers (means initial BW, 315 kg) were penned individually and fed the diets used in Trial 1. Steers fed the 100% concentrate diet received zero, four or eight pot scrubbers. From d 113 to 167, steers provided with four or eight pot scrubbers or fed the 85% concentrate diet had greater (P less than .10) gains than steers fed the 100% concentrate diet without pot scrubbers. In Trial 3, 120 steers (means initial BW, 286 kg) were grouped in 12 pens and limit-fed an all-concentrate diet for 84 d. Sixty steers were provided with six pot scrubbers each. Performance was not affected (P greater than .10) by the use of pot scrubbers during the 84-d growing phase. During the subsequent 84-d finishing phase, half the steers receiving each treatment were switched to either an 85% concentrate-15% corn silage or an 100% concentrate diet offered for ad libitum consumption.(ABSTRACT TRUNCATED AT 250 WORDS)     

Effects of diet type and metabolizable energy intake on tympanic temperature of steers fed during summer and winter seasons

A summer study and a winter study were conducted using an incomplete factorial structure in a complete randomized design. Within season, the factors studied were 1) type of diet, which included 2 levels of ME, classified as either concentrate (3.04 Mcal of ME/kg) or roughage (2.63 Mcal of ME/kg) diets, and 2) daily ME intakes (MEI) of 11, 18, and 25 Mcal of ME/d for the roughage diets and 18, 25, and 32 Mcal of ME/d for the concentrate diets. In Exp. 1 (summer study), 30 steers (5 steers/treatment combination) were used to collect tympanic temperatures (TT). In Exp. 2 (winter study), 24 steers (4 steers/treatment combination) were used to collect TT. Mean TT was 0.3°C greater for summer than winter (38.9 vs. 38.6°C, respectively; P < 0.05). Steers fed diets based on concentrate tended to display greater TT than steers fed diets based on roughage. Season × diet × hour interactions were found for TT (P = 0.01). In the winter, greater TT (P < 0.05) were found from 0900 to 1400 h when an equal amount of MEI was derived from a concentrate-based vs. roughage-based diet. In cattle fed roughage-based diets during the summer, TT = 38.63 + 0.0114?MEI, whereas for cattle fed concentrate-based diets, TT = 38.69 + 0.0114?MEI. During the winter, for cattle fed a roughage-based diet, TT = 37.65 + 0.0856?MEI - 0.0018?MEI(2), whereas for cattle fed a concentrate-based diet, TT = 35.37 + 0.2635?MEI - 0.0051?MEI(2). In summary, results demonstrate that increases in the energy of the diet resulted in increases in TT. However, the response was dependent on season of the year, with a linear response in TT for summer and a quadratic response during winter.     

Effects of barley variety fed to steers on carcass characteristics and color of meat

This study evaluated the effect of barley varieties in the diets of finishing steers on carcass composition, fat, and lean color and the fatty acid profile of subcutaneous fat. Crossbred steers (391 kg initial BW) were assigned randomly to one of five finishing diets composed primarily of corn (n = 9), Morex barley (n = 9), Steptoe barley, (n = 9), or two experimental barley varieties SM3 (n = 9) and SM5 (n = 9). Grains were cracked prior to feeding. Diets were formulated (DM basis) to be isonitrogenous (2.24% N) and isocaloric (2.01 Mcal/kg NEm and 1.35 Mcal/kg NEg). Steers were slaughtered according to industry-accepted procedures when it was visually estimated that 70% of carcasses would grade USDA Choice. After a 24-h chill at 4 degrees C, carcass quality and yield grade data were collected by trained, experienced university personnel. Objective color (L*, a*, and b*) of both the LM and subcutaneous fat were measured, and samples of subcutaneous fat were removed from the 10th- to 12th-rib region for fatty acid analysis. Diet did not affect hot carcass weight (P = 0.15), fat thickness (P = 0.58), LM area (P = 0.57), percentage of internal fat (P = 0.52), yield grade (P = 0.96), marbling (P = 0.73), or quality grade (P = 0.10). However, the LM from steers fed diets formulated with Morex and SM5 barley varieties tended to be lighter (higher L* values, P = 0.08) than the LM from steers fed the corn-based diet. Additionally, fat from steers fed corn tended to be more yellow (higher Hunter b* values, P = 0.09) than fat from steers fed barley-based diets. Although grain source had only minimal effects on the fatty acid composition of subcutaneous fat samples, pentadecanoic acid (15:0) was greater (P < 0.05) in fat from steers fed SM3 and Steptoe barley varieties than in fat from steers fed corn. Stearic acid (18:0) concentrations were higher (P < 0.05) in fat samples from steers fed corn than in those fed the experimental barley lines (SM3 and SM5). Conversely, fat samples from steers fed Steptoe and SM5 barley had greater (P < 0.05) gadoleic acid (20:1) concentrations than fat from steers fed corn or Morex variety. Although the variety/line of barley included in the finishing diet may affect LM and fat color, grain-source (barley vs. corn) had little effect on beef carcass quality and yield grades and did not greatly alter the fatty acid composition of subcutaneous fat.     

Effects of dry corn gluten feed on feedlot cattle performance and fiber digestibility

Effects of dry corn gluten feed (DCGF) on feedlot cattle performance and fiber digestibility were investigated. In Trial 1, 120 growing steers were fed corn silage-based diets containing 0, 40, 60 or 80% DCGF. Increasing levels of DCGF resulted in a curvilinear response in gain (P less than .05) and a linear increase in feed/gain (P less than .01). When the same steers subsequently were fed the same levels of DCGF in corn-based diets (Trial 2), increasing the percentage of dietary DCGF resulted in a linear decrease in gain (P less than .01) and a linear increase in feed/gain (P less than .01). In Trial 3, 46 crossbred steers were fed individually in a 2 x 2 factorial design to determine effects of 60 or 80% dietary high-moisture corn (HMC) or DCGF on feedlot cattle performance. Steers fed HMC had faster (P less than .08) and more efficient (P less than .05) gains than those fed DCGF, which had greater feed intakes (P less than .05). In Trial 4, 120 Angus crossbred steers were used to compare effects of 20 or 40% dietary HMC or DCGF on feedlot performance. Steers fed diets containing 40% HMC or DCGF had greater gains (P less than .01) and feed intakes (P less than .01) than those fed 20% diets. Steers fed HMC gained more efficiently than those fed DCGF (P less than .01). In an in situ trial, 0, 40, 60 or 80% dietary DCGF did not affect in situ DCGF DM or NDF disappearance. When DCGF was fermented in vitro in combination with corn silage, increasing the level of DCGF from 0 to 100% resulted in a linear increase (P less than .01) in 24 and 48 h NDF disappearance. These results suggest that at high dietary levels DCGF will support feedlot cattle gains that are nearly equal to those of cattle fed corn silage but somewhat less than those fed corn.