共查询到20条相似文献,搜索用时 15 毫秒
1.
Nao TSUZUKI Yoshiro ENDO Lisa KIKKAWA Kenji KOROSUE Yasuyuki KANEKO Akira KITAUCHI Hiromu KATAMOTO Yuichi HIDAKA Mitsuyoshi HAGIO Shidow TORISU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1647-1650
The performance of horses undergoing regular intense exercise is adversely affected by
oxidative stress. Thus, it is important to increase antioxidant production in horses in
order to reduce oxidative stress. Ozonated autohemotherapy (OAHT) reportedly promotes
antioxidant production. This study aimed to evaluate the effects of OAHT on antioxidant
capacity. Ten Thoroughbred horses were used in this study. After the OAHT, we collected
serum samples and measured biological antioxidant potential (BAP). We found that BAP began
to increase after the OAHT and was significantly higher in the OAHT group than at 3
(P<0.01) and 7 days (P<0.05) after OAHT than in
the control group at 3 and 7 days after starting collection of blood samples. Therefore,
it was shown that OAHT improved the antioxidant capacity of the horses. 相似文献
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Naofumi OGUSHI Kazuaki SASAKI Minoru SHIMODA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1171-1173
The effect of the antitumor drug, methotrexate (MTX), which is applied to brain tumors,
is restricted by the blood-brain barrier (BBB), which is composed of P-glycoprotein (P-gp)
and multidrug resistance associated protein (MRP). We, therefore, studied if a potent P-gp
and MRP modulator, cyclosporin A (CysA), can modulate the MTX concentration in the rat
brain. If it can, which route is more effective, intravenous or intrathecal? We
intravenously or intrathecally administered MTX to rats with or without CysA. After 6 hr,
brains and cerebrospinal fluid (CSF) were sampled, and their MTX concentrations were
compared. Each MTX concentration was determined by high-performance liquid chromatography
with UV detection. CysA had no significant affect on the MTX concentration in the brain or
CSF when MTX was intravenously injected. In contrast, when MTX was intrathecally
administered, CysA had a larger effect on the MTX concentration in the brain than in the
CSF. This indicates CysA potentiated the brain MTX concentration when MTX was
intrathecally administered. It is suggested that CysA did not potentiate the distribution
of MTX from blood into the brain, but instead potentiated the distribution of MTX from CSF
into the brain. Since chemicals in CSF generally diffuse into the brain easily, CysA
probably inhibited the excretion of MTX from the brain. This could be caused by inhibition
of P-gp or MRP at the BBB. Therefore, CysA can be a useful tool to achieve an appropriate
MTX concentration in brain. 相似文献
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Ayano OMURA Hideki ENDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):43-47
The digestive organs in decapodiform cephalopod species morphologically vary by
individual lifestyle. We examined the following six species of adult decapodiformes
cephalopods representing different habitats: Todarodes pacificus, Loligo
bleekeri, Loligo edulis, Watasenia
scintillans (pelagic), Sepia lycidas and Euprymna
morsei (benthic). L. bleekeri and L. edulis
possess a bursiform cecal sac connected to the cecum. Pelagic species have a single
digestive gland smaller than in benthic species. T. pacificus has an oval
digestive gland larger than that of L. bleekeri and L.
edulis, which possess withered-looking and smaller digestive glands. In
contrast, the digestive glands in benthic species are paired. S. lycidas
and E. morsei have well-developed and larger digestive glands than those
of the pelagic species. Well-developed digestive duct appendages are found in benthic
species. In qualification of the mass of digestive organs, pelagic species have smaller
stomachs, digestive glands and digestive ducts’ appendages than benthic species. Because
pelagic species need to swim, they may possess smaller stomachs and larger cecums for more
rapid digestion. A smaller digestive gland may have the advantage of reducing the body
weight in pelagic species for rapid swimming. In contrast, since benthic species require a
longer time for digestion than pelagic species, they compact more food in their stomachs
and absorb nutrients via more organs, such as the digestive grand and digestive duct
appendages, in addition to cecum. 相似文献
5.
Shun TAKEO Daichi SATO Koji KIMURA Yasunori MONJI Takehito KUWAYAMA Ryoka KAWAHARA-MIKI Hisataka IWATA 《The Journal of reproduction and development》2014,60(2):92-99
The aim of the present study was to address the effect of resveratrol-mediated
upregulation of sirtuin 1 (SIRT1) during oocyte maturation on mitochondrial function, the
developmental ability of oocytes and on mechanisms responsible for blockage of polyspermic
fertilization. Oocytes collected from slaughterhouse-derived ovaries were cultured in
TCM-199 medium supplemented with 10% FCS and 0 or 20 µM resveratrol (Res). We examined the
effect of Res on SIRT1 expression in in vitro-matured oocytes (Exp 1);
fertilization and developmental ability (Exp 2); mitochondrial DNA copy number (Mt
number), ATP content and mitochondrial membrane potential in matured oocytes (Exp 3); and
the time required for proteinase to dissolve the zona pellucida following in
vitro fertilization (as a marker of zona pellucida hardening), as well as on
the distribution of cortical granules before and after fertilization (Exp 4). In Exp 1,
the 20 µM Res treatment upregulated protein expression of SIRT1 in oocytes. In Exp 2, Res
treatment improved the ratio of normal fertilization and the total cell number of
blastocysts. In Exp 3, Res treatment significantly increased the ATP content in matured
oocytes. Additionally, Res increased the overall Mt number and mitochondrial membrane
potential, but the effect was donor-dependent. In Exp 4, Res-induced zona hardening
improved the distribution and exocytosis of cortical granules after in
vitro fertilization. In conclusion, Res improved the quality of oocytes by
improving mitochondrial quantity and quality. In addition, Res added to the maturation
medium enhanced SIRT1 protein expression in oocytes and improved fertilization via
reinforcement of the mechanisms responsible for blockage of polyspermic fertilization. 相似文献
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Isao IGARASHI Toshihiko MAKINO Kiyonori KAI Munehiro TERANISHI Wataru TAKASAKI Hiroshi SATOH Kazuhisa FURUHAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):113-116
We examined the localization of connexin 32 (Cx32), a component of gap
junctions, in 24-month-old male B6C3F1 mice with spontaneously occurring hepatocellular
altered foci or tumors. Immunohistochemically, Cx32-staining intensity in cell-to-cell
membranes of altered hepatocytes was decreased in eosinophilic foci and increased in
basophilic foci as compared to those in intact hepatocytes. These alterations were
enhanced in adenomas and carcinomas with both eosinophilic and basophilic cytoplasm. In
cell membranes facing on the sinusoidal portions, the intensities increased in all
lesions. Image analyses confirmed that the spot areas of Cx32 were decreased in
eosinophilic foci, but increased in basophilic foci, adenomas and carcinomas. These
results demonstrate that Cx32 shows different expression in different types of hepatic
lesions. 相似文献
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Shoko NAKAMUTA Nobuaki NAKAMUTA Yoshio YAMAMOTO Nozomi ONODERA Isato ARAKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):89-93
In this study, immunohistochemical analysis has been performed using neuronal
markers (GAP43, NCAM and PGP 9.5) to characterize the epithelial invagination in the
medial wall of the olfactory pit in the chick embryos. At stages 26–27, the epithelial
invagination was primarily composed of characteristic round-shaped cells, which were
negative for neuronal markers. These cells were also found in the medial wall of the
olfactory pit at stage 24, whereas the epithelial invagination was not observed at any
stages other than stages 26–27. The possible relationship between the round-shaped cells
and the migratory cells is discussed. 相似文献
8.
Oxazolone-induced gastrointestinal disorders enhance the oral transmission of
AA amyloidosis in mice
Hiroto KOBAYASHI Susumu IWAIDE Naoki UJIKE Tomoaki MURAKAMI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):935
Amyloid A (AA) amyloidosis is a lethal disease characterized by systemic AA amyloid deposition, and is reported in many animal species. Despite experiments have shown that AA amyloidosis can be transmitted orally, horizontal transmission and cross-species transmission are concerns, the transmission mechanism has been unknown. In this study, we examined the oral transmission efficiency of AA amyloidosis using oxazolone-induced gastrointestinal disorder mice. As a result, the upper or lower gastrointestinal disorder groups developed more severe amyloid deposition in systemic tissues than the group without gastrointestinal disorders. The results of this study suggest that gastrointestinal damage promotes the oral transmission of AA amyloidosis. 相似文献
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Sarah J. Clift Bernat Martí-Garcia Rephima M. Phaswane Emily P. Mitchell Antoinette I. Josemans Ilse Vorster Katja N. Koeppel Jeanni Fehrsen 《Journal of veterinary diagnostic investigation》2021,33(6):1079
Theileria parasites commonly infect African wild artiodactyls. In rare roan (Hippotragus equinus) and sable (H. niger) antelopes, Theileria sp. (sable)-associated calf mortalities constrain breeding programs. The pathogenicity of most leukocyte-transforming Theileria spp. originates in their invasion of and multiplication in various mononuclear leukocytes, the transformation of both infected and uninfected leukocytes, and their infiltration of multiple organs. Understanding the pathogenesis of theileriosis can be improved by the use of immunohistochemistry (IHC) to identify the localization of the parasites in tissue sections. Our aim was to develop a reproducible IHC assay to detect leukocyte-associated Theileria parasites in formalin-fixed, paraffin-embedded roan and sable tissues. Polyclonal antibodies were purified from the sera of 5 roans from an area endemic for Theileria sp. (sable) and tested for IHC reactivity in 55 infected and 39 control roan and sable antelopes, and for antigen and species cross-reactivity in an additional 58 cases. The 3 strongest antibodies consistently detected intraleukocytic theilerial antigens in known positive cases in roan and sable antelopes, and also detected other Theileria spp. in non-hippotraginid wild artiodactyl tissues. The antibodies did not cross-react with other apicomplexan protozoa, with the exception of Cryptosporidium. Given that PCR on its own cannot determine the significance of theilerial infection in wild ruminants, IHC is a useful laboratory test with which to confirm the diagnosis in these species. 相似文献
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Kanae NIIMI Motoko MORIMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):573
To eliminate pathogens, the initiation of an appropriate immune response is critical. When the gastrointestinal nematode, Heligmosomoides polygyrus (Hp), invades the small intestine, a type-2 cytokine response is initiated; however, this response is not sufficient to clear the infection, and chronic infection can ensue. In this study, the host defense against Hp was investigated in mice with a focus on the role of CD4+ T cells. To this end, tissues from the small intestine and mesenteric lymph node (MLN) were collected every day from just after infection until Day 5 because many previous studies have described the later stages of infection from Day 8 to Day 12, during which Hp returns to the lumen and Th2 cytokine expression reaches its peak. In this study, we focused on investigating the initiation of the type-2 immune response. Our results indicated that the larvae encysted by Day 3. Increased type-2 cytokine gene expression started in the small intestine before Day 2 and increased again on Day 5. Interferon (IFN) γ increased significantly on the second day. Flow cytometry and gene expression analysis of MLN cells revealed that CD4+ T cells were not activated until Day 4. These results suggested that innate immune cells in submucosa are activated immediately after infection, but CD4+ T cells accumulate in the cyst zone later. In addition, IFNγ may have an important role in converting type-2 cytokine-producing cells from innate cells to CD4+ T cells. 相似文献
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Yoshie MANABE Makoto TOCHIGI Akiyoshi MORIWAKI Sakae TAKEUCHI Sumio TAKAHASHI 《The Journal of reproduction and development》2013,59(4):398-404
Reproductive functions decline with the onset of diabetes in female mice. Diabetic mice
have smaller uteri with an underdeveloped endometrium, suggesting diminished
estrogen-induced growth. We aimed to clarify the changes in the estrous cycle and in
insulin-like growth factor 1 (IGF1) expression in the uteri of streptozotocin
(STZ)-treated diabetic mice, because IGF1 is one of the main growth factors involved in
estrogen-induced uterine growth. ICR female mice were intraperitoneally administered STZ
(10 mg/100 g BW), and blood glucose levels were determined. Mice with blood glucose levels
> 200 mg/dl were classified as diabetic mice. The onset of diabetes was associated with
acyclic estrous cycles. Diabetes was also induced with STZ in ovariectomized mice. Uterine
Igf1 mRNA levels were reduced in ovariectomized STZ-treated diabetic
mice. Estrogen is known to stimulate Igf1 mRNA expression in the uterus,
but estrogen action was abolished in the uteri of STZ-treated diabetic mice. mRNA
expressions of estrogen receptor α (ERα) and steroid hormone receptor coactivators
(SRC-1/Ncoa1, SRC-2/Ncoa2,
SRC-3/Ncoa3 and CBP/p300/Crebbp) were reduced in the
uteri of ovariectomized STZ-treated diabetic mice. The present study demonstrates that
diabetes induces a decline in female reproductive functions in mice. Igf1
expression in ovariectomized diabetic female mice was decreased, and decreased
responsiveness to estrogen in the uteri of diabetic mice is probably associated with a
reduction in ERα and steroid receptor coactivator mRNA expression. 相似文献
15.
Andr Tavares SOMMA Adriane STEUERNAGEL Eduardo Alberto PULIDO-MURILLO Hudson Alves PINTO Larissa REIFUR Bret A. MOORE Fabiano MONTIANI-FERREIRA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(10):1447
In this study, clinical, parasitological and histopathological findings of thirteen kelp gulls (Larus dominicanus) found infected with eyeflukes in Brazil are presented. Parasites detected in the ventral conjunctival fornix were identified as Philophthalmus lachrymosus [mean intensity of infection: 16 (5–36) worms/bird]. Eleven birds (85%) presented signs of systemic disease, such as emaciation, dehydration and depressed consciousness. Conjunctival hyperemia was observed in 22 eyes (85%). Keratitis, corneal ulcers, corneal abscess and chemosis were also detected in some eyes (4–8%). Histopathologic lesions, likely due to the parasite attachment to the conjunctiva, were found in the eyes of one infected bird that died from unrelated causes. Philophthalmosis by P. lachrymosus is here reported as a clinically relevant eye disease in kelp gulls. 相似文献
16.
Shinichi Onishi Atsuko Murai Aki Kito Yuka Kawashima Yusuke Ohmori Atsuhiko Kato 《Journal of toxicologic pathology》2022,35(3):275
Vestibular organs consist of the maculae staticae, which are located in both the utricle and saccule, as well as the semicircular ducts and their ampullas. There have been no reports on specimen preparation methods for vestibular organs, including maculae staticae or semicircular ducts. In this study, we investigated highly reproducible methods of preparing vestibular organ specimens for histopathological examinations. We established a method that allows researchers to observe the utricle and saccule, including otoliths, the ampulla of a semicircular duct, and parts of semicircular ducts. This highly reproducible method is useful for histopathological analysis of mice with symptoms of abnormal equilibrium caused by medical toxicity and genetic modification. 相似文献
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Anaphylactic reaction rarely occurs after the 1st injection of
penicillin-streptomycin in cattle. Immediately following intramuscular injection
of a Holstein-Friesian cow with penicillin-streptomycin, the cow showed abnormal
clinical signs that included respiratory distress, urticaria, and lacrimation.
Recovery took approximately 2 h after intramuscular injection of dexamethasone.
This report presents clinical findings recorded 20 and 40 h later. 相似文献