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1.
David M. Soderlund 《Pesticide biochemistry and physiology》1979,12(1):38-48
The potent pyrethroid insecticide NRDC 157 (I; 3-phenoxybenzyl (1R, cis)-3 - (2,2-dibromovinyl)-2,2-dimethylcyclopropanecarboxylate) and its insecticidally inactive 1S, cis enantiomer (II) have similar cuticular penetration rates and are detectable in the hemolymph, nerve cord, fat body, and midgut of the American cockroach, Periplaneta americana, within 2 hr of topical application at 0.17 μg/g, a just-lethal dose of I. At this dose, both enantiomers show similar distribution patterns in these tissues, but symptoms of intoxication are seen only with I. Steady state concentrations of both enantiomers in the hemolymph and nerve cord are between 1.2 × 10?7 and 1.7 × 10?7M. I and II are not detectably hydrolyzed by fat body, nerve cord, and hemolymph preparations and are not detectably oxidized by fat body preparations in vitro, but a very low oxidation rate is measured for II, but not I, in nerve cord preparations. These results do not demonstrate a pharmacokinetic basis for the difference in insecticidal activity between enantiomers and therefore it is likely that the site of pyrethroid action is stereospecific. The use of the inactive enantiomer II as a model to study the effects of dose on penetration and distribution in the absence of symptoms is explored. 相似文献
2.
The penetration and degradation of six pyrethroids were examined in the twospotted spider mite, Tetranychus urticae Koch, and the results were related to their toxicity as measured by inhibition of respiration using the Warburg technique and mortality using the slide-dip bioassay. FMC-54800 [1,1′-biphenyl-3ylmethyl cis-3-(2-chloro-3,3,3-trifluoro-1-propenyl)-2,2-dimethylcyclopropanecarboxylate] was the most toxic pyrethroid to the mites based on both respiration and mortality studies. It and flucythrinate had the highest pharmacokinetic efficiency as determined by delivery and maintenance of internal levels of parent compounds. Permethrin, fenvalerate, and fluvalinate were intermediate in pharmacokinetic efficiency, whereas cypermethrin was significantly lower. The highest intrinsic activity, as estimated by the percentage inhibition of respiration per microgram of internal parent, was possessed by cypermethrin and FMC-54800. Fenvalerate and fluvalinate had intermediate levels, while permethrin and flucythrinate had significantly lower capacities to inhibit respiration. The combination of relatively high pharmacokinetic efficiency and intrinsic activity of FMC-54800 appeared to be responsible for its high toxicity. In addition to these findings, differences in the kinetics for cis and trans isomers were observed for permethrin but not cypermethrin. This study has yielded evidence that acaricidal activity of pyrethroids can be enhanced by optimizing the structure for increased pharmacokinetic efficiency and increased intrinsic activity. 相似文献
3.
Altosid (I) was found to have an ED50 of 0.026 μg/g in T. molitor and 5.0 μg/g in O. fasciatus. The juvenilizing activity of I in T. molitor was increased by co-treatment with piperonyl butoxide (PB) and triorthocresyl phosphate (TOCP). The activity of I in O. fasciatus, was considerably decreased by PB and TOCP indicating the blocking of an activation reaction. The presumptive metabolities of I, isopropyl 11-hydroxy-3,7,11-trimethyldodeca-2,4-dienoate, (II), 11-methoxy-3,7,11-trimethyldodeca-2,4-dienoic acid (III), and 11-hydroxy-3,7,11-trimethyldodeca-2,4-dienoic acid (IV) were tested for juvenilizing activity in O. fasciatus and II was found to be four times more active than I. IV was more active than III but less active than I. Co-treatment of II with PB or TOCP did not decrease activity, indicating that II is a true activation product. Uptake studies with 14C-I showed that the difference in activity between the two insects was not due to differences in uptake. Forty-eight hours after treatment 30% of I was lost from the cuticle by evaporation. Metabolic studies showed that PB inhibited the formation of II and TOCP inhibited the formation of III in T. molitor while in O. fasciatus both synergists inhibited the formation of II. 相似文献
4.
Derek W. Gammon Mark A. Brown John E. Casida 《Pesticide biochemistry and physiology》1981,15(2):181-191
Pyrethroids are divided into two classes (Types I and II) based on their effects on the cercal sensory nerves recorded in vivo and in vitro and on the symptomology they produce in dosed cockroaches, Periplaneta americana. Type I compounds include pyrethrins, S-bioallethrin, [1R,cis]resmethrin, kadethrin, the 1R,trans and 1R,cis isomers of tetramethrin, phenothrin, and permethrin, and an oxime O-phenoxybenzyl ether. Electrophysiological recordings from dosed individuals reveal trains of cercal sensory spikes and sometimes also spike trains from the cercal motor nerves and in the CNS. Low concentrations of these pyrethroids act in vitro to induce repetitive firing in a cercal sensory nerve following a single electrical stimulus. This in vitro measurement, standardized for evaluating structure-activity relationships, shows that only 1R, insecticidal isomers are highly effective neurotoxins. The most potent compounds on the isolated nerve are [1R,trans]- and [1R,cis]tetramethrin, each active at 3 × 10?13M. The poisoning symptoms of Type I compounds are restlessness, incoordination, hyperactivity, prostration, and paralysis. Type II compounds include [1R,cis,αS]- and [1R,trans,αS]cypermethrin, deltamethrin, and [S,S]fenvalerate. These α-cyanophenoxybenzyl pyrethroids do not induce repetitive firing in the cercal sensory nerves either in vivo or in vitro; moreover, they cause different symptoms, including a pronounced convulsive phase. Two other pyrethroids with an α-cyano substituent, i.e., fenpropathrin and an oxime O-α-cyanophenoxybenzyl ether, are classified as Type I based on their action on a cercal sensory nerve but the symptoms with these compounds resemble Type II. The two classes of pyrethroid action evident with the cockroach are discussed relative to their neurophysiological effects and symptomology in other organisms. 相似文献
5.
Martin H. Evans 《Pesticide biochemistry and physiology》1976,6(6):547-550
Isolated sartorius nerve-muscle preparations from Rana temporaria were irrigated in a chamber. Resting muscle membrane potentials and evoked end-plate potentials were recorded via intracellular microelectrodes. Multiple end-plate potentials appeared after the preparation had been exposed to a suspension, in Ringer's solution, of the toxic synthetic pyrethroid: 5-benzyl-3-furylmethyl (+)-cis-chrysanthemate (NRDC 119). It is thought that this effect was due to repetitive activity arising in the motor nerve fibers. The pyrethroid had no effect on the muscle resting potential nor on the amplitude or time course of the initial evoked end-plate potential. 相似文献
6.
Metabolism in mice of the separated cis- and trans-isomers of the pyrethroid insecticide cypermethrin (NRDC 149), (RS)-α-cyano-3-phenoxybenzyl (1RS)-cis, trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylate, was investigated in each case with preparations that were 14C-labelled in the benzyl and cyclopropyl moieties. Radioactivity from the trans-isomer was mainly excreted in the urine and that from the cis-isomer in the faeces. Elimination of both isomers was rapid except for a small portion (approximately 2%) of the cis-isomer which was released from the fat with a half-life of approximately 13 days. Metabolism of cypermethrin occurred mainly by ester cleavage and elimination of the cis- and trans-3-(2,2-dichlorovinyl)-2,2-dimethyl- cyclopropanecarboxylic acid moieties as glucuronide conjugates. The α-cyano-3-phenoxybenzyl alcohol released by ester cleavage was mainly converted to 3-phenoxy-benzoic acid which was partly eliminated unchanged, partly conjugated with aminoacids (mainly taurine) and glucuronic acid, and partly oxidised to 3-(4-hydroxyphenoxy) benzoic acid which was excreted as the sulphate conjugate. Metabolites retaining the ester linkage were formed by hydroxylation at various sites in the molecule with more hydroxylation of the cis- than of the trans-isomer occurring. 相似文献
7.
Four synergists are used to evaluate the relative contribution of esterases and oxidases in the metabolism of four pyrethroids, the (+)-trans- and (+)-cis-isomers of resmethrin and tetramethrin, by five insect species and by mice. Three of these compounds are known pyrethroid synergists, S,S,S-tributyl phosphorotrithioate acting as an esterase inhibitor and piperonyl butoxide and O-(2-methylpropyl) O-(2-propynyl) phenylphosphonate acting as oxidase inhibitors. The fourth synergist, 1-naphthyl N-propylcarbamate, is an esterase inhibitor selected by screening 65 candidate esterase and oxidase inhibitors for maximal potency in synergizing the toxicity of trans-resmethrin to milkweed bugs. Naphthyl propylcarbamate synergizes the toxicity of trans-resmethrin and -tetramethrin to milkweed bugs, cockroaches, houseflies, cabbage loopers, and mealworms but not to mice. The persistence of trans-resmethrin in milkweed bugs treated by injection is increased by the esterase inhibitors while that of cis-resmethrin is increased by the oxidase inhibitors. The optimal synergist varies with the species and the pyrethoid, being related to both the nature of the pyrethroid alcohol moiety and the trans- or cis-configuration of the acid moiety. This probably results from species variations in the relative significance of esterases and oxidases in pyrethroid detoxification. 相似文献
8.
Intact house flies were observed during poisoning caused by several pyrethroid and DDT-type insecticides. The two insecticide classes could be generally distinguished from each other based on differences in symptoms and several physiological correlates. Both insecticide types caused motor unit repetitive backfiring, but the temporal development and stability of repetitiveness were distinctly different between the two classes. Repetitive backfiring always disappeared at low temperatures, but DDT-type backfiring disappeared at lower temperatures than the pyrethroids. trans-Tetramethrin caused a threshold increase in flight motor nerve endings which did not occur in DDT or trans-Barthrin poisoning. Pyrethroids caused “uncoupling” of the flight motor pattern, while DDT-types did not. trans-Barthrin, a methylenedioxyphenyl pyrethroid, was unique in causing both symptoms and physiological aberrations which more closely resembled those of the DDT-types than the pyrethroids. 相似文献
9.
The structure-activity relationships of 30 synthetic pyrethroids have been studied by measurements of their direct action on isolated crayfish nerve cord. The concentrations of pyrethroids used to increase the frequency of spontaneous discharges to 200% of the control (NS200), those to decrease the frequency back to the control level, and those to further decrease the frequency to 10% of the control were measured as indexes of the nerve action. These values did not necessarily run parallel with those for the lethal dose 50 or the knock-down 50. Large differences in NS200 were found among optical isomers of tetramethrin and phenothrin, and there was a synergism with respect to the nerve stimulating action between (+) and (?) forms of tetramethrin. (+)-trans-Permethrin was unique in that it exhibited a potent insecticidal action with a very weak nerve action. It is necessary to compare the direct action on the target site for the purpose of establishing the true structure-activity relationships of synthetic pyrethroids. 相似文献
10.
Deltamethrin and NRDC 157, pyrethroid insecticides that produce different poisoning syndromes in mammals, enhanced veratridine-dependent, sodium channel-mediated 22Na+ uptake in mouse brain synaptosomes. Concentrations producing half-maximal enhancement were 2.5 × 10?8M (deltamethrin) and 2.2 × 10?7M (NRDC 157). This effect was stereospecific: The nontoxic 1S enantiomers had no significant effect on veratridine-dependent activation. At high deltamethrin concentrations, enhancement was maximal at 5 × 10?5?1 × 10?4M veratridine. Pyrethroid enhancement was completely blocked by 5 × 10?6M tetrodotoxin, and neither pyrethroid affected 22Na+ uptake in the absence of veratridine at concentrations up to 1 × 10?5M. The relative potencies of deltamethrin and NRDC 157 in the synaptosomal sodium channel assay agree well with their relative acute toxicities to mice when administered by intracerebral injection. These findings demonstrate that pyrethroids exemplifying both characteristic poisoning syndromes are potent, stereospecific modifiers of sodium channel function in mammalian brain. 相似文献
11.
Pyrethroid carboxyesterase which hydrolyzes the esters of chrysanthemumic acid was purified from rat liver microsome by cholic acid solubilization, ammonium sulfate fractionation, heat treatment, and DEAE-Sephadex A-50 column chromatography. The 45-fold purified enzyme (38% yield) is likely to consist of single protein, as evidenced by polyacrylamide gel disc electrophoresis and Sephadex G-100 column chromatography, and had a molecular weight of approximately 74,000 and a Km of 0.21 mM. It is susceptible to inhibition by organophosphates and carbamate insecticides and insensitive to pCMB, mercuric ion, and cupric ion. It is capable of hydrolyzing trans isomers of synthetic pyrethroids much more rapidly (five to ten times) than the cis counterparts. The purified pyrethroid carboxyesterase is apparently identical in nature with malathion carboxyesterase and with p-nitrophenyl acetate carboxyesterase. 相似文献
12.
Charles O. Abernathy Kenzo Ueda Judith L. Engel Loretta C. Gaughan John E. Casida 《Pesticide biochemistry and physiology》1973,3(3):300-311
An esterase or esterases in acetone powder preparations of mouse liver microsomes hydrolyze the cyclopropanecarboxylate ester linkage of pyrethroid insecticide chemicals derived from primary alcohols. The rate of cleavage of (+)-trans-chrysanthemates with various alcohol moieties decreases in the following order: 5-propargyl-2-furylmethyl; 5-benzyl-3-furylmethyl (bioresmethrin); 3-phenoxybenzyl; tetrahydrophthalimidomethyl esters. The hydrolysis rate of benzylfurylmethyl esters with various acid moieties decreases in the order: (+)- or (?)-trans-chrysanthemate; (+)-trans-ethanochrysanthemate; tetramethylcyclopropanecarboxylate; (+)- or (?)-cis-chrysanthemate or (+)-cis-ethanochrysanthemate. The trans-isomers of chrysanthemates and ethanochrysanthemates are hydrolyzed from 2.6- to more than 50-fold more rapidly than the corresponding cis-isomers. This enzyme system does not hydrolyze secondary alcohol esters, i.e., allethronyl (+)-trans- and (+)-cis-chrysanthemates.On intraperitoneal administration to mice, the (+)-trans-chrysanthemate and -ethanochrysanthemate of benzylfurylmethanol are of very low toxicity relative to the corresponding (+)-cis-isomers and the tetramethylcyclopropanecarboxylate. S,S,S-tributyl phosphorotrithioate (DEF) pretreatment increases the toxicity of these five compounds by 2.6- to more than 188-fold, with the exception of bioresmethrin whose toxicity is not altered. When the toxicity is increased, it is probably the result of esterase inhibition since DEF strongly inhibits the esterase activity of fresh liver microsomes while the mixed-function oxidase system remains active. The oxidase system metabolizes the chrysanthemates more rapidly than the ethanochrysanthemates of benzylfuryl-methanol. Depending upon the pyrethroid involved, the esterase or the mixed-function oxidase system, or both may be responsible for limiting the toxicity of these pyrethroids to mice. 相似文献
13.
Studies of the degradation of the pyrethroid insecticide cypermethrin (NRDC 149) and its cis- and trans-isomers (NRDC 160 and NRDC 159, respectively), have been extended. In soils stored in the laboratory for up to 52 weeks, cypermethrin continued to be degraded by hydrolysis and oxidation. A previously unidentified product has now been identified as the dicarboxylic acid 3-(2, 2-dichlorovinyl)-1-methylcyclopropane-1, 2-dicarboxylic acid. Comparative experiments carried out under indoor and outdoor conditions showed that essentially the same products were formed under these different conditions. However, α-carboxy-3-phenoxybenzyl 3-(2, 2-dichlorovinyl)-2, 2-dimethyl-cyclopropanecarboxylate was one minor product detected only under outdoor conditions. Evidence is presented for the further degradation of bound residues arising in soil from cypermethrin treatments. There was limited uptake of the radiolabel into wheat grown in soil containing radiolabelled bound residues. 相似文献
14.
A GC-MS method capable of completely separating the four pairs of diastereoisomers of cyfluthrin is presented and the method used to show that isomerisation of the cyfluthrin enantiomers occurs in methanol. This methanol-induced isomerisation could also be demonstrated by bioassays using water fleas. The biological activities of the various cyfluthrin isomers contained in the commercial products cyfluthrin and beta-cyfluthrin were assayed using several strains of lepidopteran larvae including Plutella xylostella, Heliothis virescens and Spodoptera frugiperda. With the susceptible strains, the efficiencies of the isomers mixtures of cyfluthrin and beta-cyfluthrin were shown to obey the rules of additivity. However, in tests with a resistant strain of P. xylostella originating from Thailand, the ‘inactive’ isomer III acted synergistically with the active isomer IV. Resistance factors in strains of H. virescens and P. xylostella were found to be higher with cis than with trans isomers. This probably contributes to the superior action of cyfluthrin and beta-cyfluthrin against various pests of agricultural importance since the commercial products contain a high content of trans isomers (‘high trans pyrethroids’). 相似文献
15.
Isaac Ishaaya Anna Elsner K. R. Simon Ascher John E. Casida 《Pest management science》1983,14(4):367-372
The potency of six dietary pyrethroids, as toxicants and inhibitors of weight gain in first- and fourth-instar Tribolium castaneum (Herbst) larvae, decreased in the order of cis-cypermethrin and deltamethrin > trans-cypermethrin and cis-permethrin > fenvalerate and trans-permethrin. Dosages that reduced larval weight also delayed pupation and emergence, probably due to their antifeeding activity. Three oxidase inhibitors (piperonyl butoxide, O, O-diethyl O-phenyl phosphorothioate, and O-isobutyl O-prop-2-ynyl phenylphosphonate), at a dietary concentration of 100 mg kg?1, had little or no effect on the toxicity of trans-permethrin, but strongly synergised the toxicity of cis-cypermethrin by about 3-, 3- and 10-fold, respectively. Piperonyl butoxide also synergised the toxicity of cis-permethrin, trans-cypermethrin and deltamethrin, but not that of fenvalerate. On the other hand, an esterase inhibitor, profenofos, did not enhance the potency of any of the α-cyano-3-phenoxybenzyl pyrethroids. Oxidases appear to be more important than esterases in pyrethroid detoxification by T. castaneum larvae. 相似文献
16.
M.E. Schroeder D.L. Shankland R.M. Hollingworth 《Pesticide biochemistry and physiology》1977,7(5):403-415
Dieldrin and two of its metabolites, 6,7-trans-dihydroaldrindiol, and 6,7-cis-dihydroaldrindiol, were studied with regard to their toxicity to the American cockroach, effects on ganglia of the ventral nerve cord, and penetration into the ventral nerve cord of poisoned cockroaches. An approximate LD50 for injected doses of dieldrin was 0.45 mg/kg. After injection at 115 mg/kg, the trans isomer of aldrin diol caused about 70%, and the cis isomer about 50% mortality. Injected doses of 40 mg/kg of the three compounds appeared in the ventral nerve cord to the extent of 0.13–0.26% of the doses. Dieldrin was more potent, but slower acting than the diols in causing synaptic after-discharge and elevated spontaneous activity in isolated nerve cords. The results are discussed in relation to other studies on these compounds. It was concluded that, in the American cockroach, dieldrin, rather than either of the diols, is the insecticidal agent in dieldrin poisoning, and that metabolic conversion of dieldrin to the cis and/or trans aldrindiol constitutes a detoxification. 相似文献
17.
《Pesticide biochemistry and physiology》1986,25(3):295-305
Twenty-five synthetic pyrethroids and related chemicals were assessed for their effects on the uptake of Ca2+ (as 45Ca2+) by crude homogenates prepared from crayfish (Procambarus clarkii) nerve cords and 20 were assessed on the uptake by homogenates prepared from lobster (Panulirus japonicus) axons. A parabolic relationship was demonstrated between inhibition of Ca2+ uptake for this series of chemicals and lipophilicity (log P) in both species when tested at 5 μM. Optimal log P for maximal inhibition was located at about 6.6 and 84(±6)% inhibition was obtained with resmethrin. Compounds of higher or lower log P were either weaker inhibitors, not inhibitors or occasionally resulted in slightly increased levels of Ca2+ uptake. No direct correlations between the potential for the pyrethroids to inhibit Ca2+ uptake and the potential for these agents to increase the frequency of spontaneous discharges in crayfish nerve cords, to induce repetitive firing in American cockroaches, or the lethality to cockroaches or to any other neurophysiological or toxicity parameter could be established. It was concluded that although some synthetic pyrethroids are moderately potent inhibitors of Ca2+ uptake into nerve cord and axonic preparations (i.e., I50 for trans-resmethrin equals 1 μM) this inhibition alone does not relate to the neurophysiological changes in isolated nerve preparations or to the toxicity of these agents in insects. 相似文献
18.
The penetration, excretion, and metabolism of topically applied [14C]permethrin and [14C]cypermethrin have been examined in larvae of the porina moth Wiseana cervinata to determine the factors which affect body levels of unchanged pyrethroids. Metabolism was by hydrolysis and to a lesser extent oxidation and the primary metabolites were quickly conjugated to water-soluble products. Little excretion occurred and body levels of unchanged pyrethroids were dependent on the interaction of penetration and metabolism. cis-Cypermethrin was more resistant to metabolism than trans-cypermethrin and cis- and trans-permethrin. trans-Permethrin most readily penetrated into larvae. The body levels of unchanged permethrin were enhanced by pretreatment of larvae with the metabolic inhibitors carbaryl or piperonyl butoxide. Tolerance of the pasture pest porina to the synthetic pyrethroids is discussed in relation to these findings. 相似文献
19.
Adult Rana pipiens pipiens (Shreber) are highly sensitive to insecticidal α-cyano-3-phenoxybenzyl esters administered subcutaneously, i.e., LD50 0.13–0.35 mg/kg for deltamethrin and the most potent isomer of each of cis-cypermethrin, fenpropathrin, and fenvalerate and 0.65 mg/kg for (1R,αS)-trans-cypermethrin. Pyrethroids lacking the α-cyano substituent [pyrethrins, S-bioallethrin, kadethrin, and the Cis- and trans-isomers of (1R)-tetramethrin, (1RS)-resmethrin, (1R)-phenothrin, and (1R)-permethrin] vary greatly in their toxicity (LD50 0.14 to > 60 mg/kg) and the trans isomers are much less toxic than the corresponding cis isomers. The trans/cis specificity is due in large part to relative detoxification rates based on synergism studies with the resmethrin and permèthrin isomers and liver pyrethroid esterase assays with the permethrin and cypermethrin isomers. Poisoning by the noncyano compounds involves hyperactivity and tremors whereas by the cyanophenoxybenzyl esters involves tonic seizures and choreoathetosis, i.e., types I and II syndromes, respectively. Picrotoxinin, t-butylbicyclophosphate, and five other small cage compounds give a third type of syndrome with clonic seizures. Diazepam and its 2′-fluoro-4-methyl-4,5-dihydro analog (RO 5-3636) are more effective than 23 other compounds tested in protecting against deltamethrin toxicity. Diazepam is most effective in alleviating the Type II syndrome, intermediate with the type I syndrome, and is not active with picrotoxinin. 相似文献
20.
Abstract The toxicity of a number of topically applied pyrethroids has been tested in the laboratory against three species of locusts and parathion-resistant and susceptible strains of the Egyptian cotton leafworm. Bioresmethrin, resmethrin and 5-benzyl-3-furylmethyl (+)-cis-chrysanthemate (NRDC 119) proved to be extremely active against one or more of these pests and there were small but noteworthy improvements with the synergists sesamex and TBTP. The field potential of the pyrethroids is discussed against the background of environmental problems associated with some of the insecticides in current use. 相似文献