The effect of temperature on the clearance of intraperitoneally-injected gonadotropin in the goldfish Carassius auratus

The serum half-disappearance time ($\text{t}{}_{\mathrm{<mtext>1</mtext><mtext>2</mtext>}}$), metabolic clearance rate and volume of distribution of intraperitoneally administered carp gonadotropin (cGtH) and endogenous GtH levels were determined in sexually mature male and female goldfish, Carassius auratus maintained at 12 ± 1°C or 20 ± 1°C. The results indicated that the rate of serum uptake of the injected cGtH from the peritoneal cavity was greater at 20°C than at 12°C in sexually mature male goldfish. Although increased endogenous serum GtH levels and decreased values of serum $\text{t}{}_{\mathrm{<mtext>1</mtext><mtext>2</mtext>}}$ were associated with the elevated temperature, there was no difference in any of the parameters between sexually mature male and female goldfish acclimated to 12°C.     

Rearing temperature affects Senegalese sole (Solea senegalensis) larvae protein metabolic capacity

The present work examined the short- and long-term effects of three rearing temperatures on protein metabolism and growth trajectories of Senegalese sole larvae using ¹⁴C-labelled Artemia protein as feed. A first feeding trial was performed on larvae reared at 15, 18 and 21 °C (at 26, 17 and 14 days post-hatching (dph), respectively) and a second trial conducted on post-larvae after transfer to the same rearing temperature (~20 °C) (49, 35 and 27 dph, in larvae initially reared at 15, 18 and 21 °C, respectively). Temperature greatly influenced larvae relative growth rate (RGR) and survival, since growth at 15 °C was severely depressed. Protein digestibility and retention was highest at 18 °C during the first trial (85.35 ± 1.16 and 86.34 ± 2.33 %, respectively). However, during the second trial, post-larvae from 15 °C had the highest feed intake and protein digestibility (3.58 ± 1.54 and 75.50 ± 1.35 %, respectively), although retention was similar between treatments. Furthermore, after transfer to 20 °C larvae from 15 °C experienced compensatory growth, which was observed until 121 dph, and confirmed by RGR values, which were significantly higher at 15 ºC than at 21 ºC or 18 ºC. Results from the present study show that Solea senegalensis larval development, survival and protein digestion and retention are highly affected by thermal history.     

Research on intensive aquaculture: Proceedings of a German-Israeli seminar on aquaculture held from March 10 to 11, 1980 at Shefayim,Israel. H. Rosenthal and O.H. Oren (Editors). European Mariculture Society,Belgium, Special Publication No. 6, 1981. ii + 277 pp., BFr. 1150

A solar-heated greenhouse containing recirculating fish production systems was used to culture Tilapia nilotica and T. aurea. Fish production was maximized by sequential rearing, grading, and restocking of fish at high densities to utilize each system fully. Marketable-size (167 g) fish were produced from 29-g fingerlings in 137 days. No reproduction was recorded.Spawning in February was induced naturally by a $\text{16}\text{L}\text{8}\text{D}$ photoperiod, water temperature of 24°C, and reducing stock density.     

Influence combinee des facteurs temperature et salinite sur la metamorphose et la croissance larvaire de la crevette rose Palaemon serratus (Pennant) (Crustacea,Decapoda, Palaemonidae)

The rate of metamorphosis of larvae, duration of larval stages and carapace lengths of postlarvae were measured in the common prawn, Palaemon serratus, reared in 30 different combinations of temperature and salinity. Temperature varied from 13°C to 29°C and salinity from 13‰ to 43‰. When salinity.was 13‰, this species was able to metamorphose at a temperature of 21°C. For salinities of 19‰, 25‰, 31‰, 37‰ and 43‰, the temperature range over which metamorphosis occurs extends from 17°C to 25°C. The equation of Van't Hoff can be adapted to calculate the relation between the rearing rate, which is the converse of the duration of larval stages, and the water temperature. The Q₁₀ values vary from 1.39 minimum at a salinity of 37‰ to 2.28 maximum at 31‰. Neither the effect of temperature (at 0.1%) nor that of salinity (at 1%) is significant for the carapace lengths of postlarvae.Tridimensional models of the combined effects of temperature and salinity on the rate of metamorphosis and on the number of metamorphosed postlarvae appearing daily were constructed for this species.     

Toxic levels of mercury for sequential larval stages of Macrobrachium rosenbergii (de Man)

The toxicity of mercury for sequential stages of Macrobrachium rosenbergii (de Man) larvae was studied under static water conditions at a salinity of 12‰ and temperature of 25.5 – 27.0°C. The results revealed that stage 1 larvae had the lowest threshold lethal concentration (TLC) of mercury, 0.041 ppm Hg, while the post-larvae had a TLC of 0.325 ppm Hg. There was an abrupt increase in the TLC of mercury after the larvae had reached stage 5. The lower TLC prior to stage 5 is most probably due to the more frequent moulting and the relatively thinner cuticle of the larvae.     

Temperature effects on acute copper toxicity to juvenile channel catfish Ictalurus punctatus

An experiment was conducted to evaluate the effect of temperature on the acute toxicity of copper sulfate to juvenile channel catfish (Ictalurus punctatus). Alkalinity and hardness were 30 and 26 mg l⁻¹, respectively. The 7–10-g fingerlings were acclimated in four climate chambers at test temperatures of 21, 23, 25, and 27 °C for 2 weeks. After determining an acutely lethal copper dose, aerated tanks of 7.6 l were placed in each climate chamber in quadruplicate. To each, seven acclimated channel catfish fingerlings were added. The 72-h cumulative mortality from 20 mg l⁻¹ copper sulfate was significantly lower in the 27 °C treatment (25%) compared to the 21, 23, and 25 °C treatments, where cumulative mortality was 79–96%. Total mortality at 72 h was significantly correlated (P<0.05) with decreasing temperatures (r=−0.91). This provides evidence of an inverse relationship between toxicity of copper sulfate and water temperature. Survival time, however, decreased with increasing temperatures.     

Molecular endocrine changes of Gh/Igf1 axis in gilthead sea bream (<Emphasis Type="Italic">Sparus aurata</Emphasis> L.) exposed to different environmental salinities during larvae to post-larvae stages

The influence of acclimation of the euryhaline gilthead sea bream (Sparus aurata) larvae/post-larvae to brackish water on growth, energetic contents, and mRNA levels of selected hormones and growth-regulating hypothalamic neurohormones was assessed. Specimens from 49 days post-hatching were acclimated during 28 days to two different environmental salinities: 38 and 20 psu (as brackish water). Both groups were then transferred to 38 psu and acclimated for an additional week. Early juveniles were sampled after 28 days of acclimation to both salinities and one week after transfer to 38 psu. Pituitary adenylate cyclase-activating peptide (adcyap1; pacap), somatostatin-I (sst1), growth hormone (gh1), insulin-like growth factor-I (igf1), and prolactin (prl) mRNA expression were all studied by QPCR. Post-larvae acclimated to 20 psu showed better growth performance and body energetic content than post-larvae maintained at 38 psu. prl, adcyap1, and igf1 mRNA expression levels increased in 20-psu-acclimated post-larvae but decreased upon transfer to 38 psu. GH1 expression did not show significant changes under both experimental conditions. Our results suggested an enhanced general performance for post-larvae in brackish water, supported by the actions of adcyap1, igf1, and prl.     

Growth performance,oxidative stress,and non-specific immune responses in juvenile sablefish, <Emphasis Type="Italic">Anoplopoma fimbria</Emphasis>, by changes of water temperature and salinity

Juvenile sablefish, Anoplopoma fimbria (mean length 15.5 ± 1.9 cm, mean weight 68.5 ± 4.8 g), were used to evaluate the effects on growth, oxidative stress, and non-specific immune responses by changes of water temperature (8, 10, 12, 14, 16, 18, and 20 °C) and salinity (100 (35.0), 90 (31.5), 80 (28.0), 70 (24.5), 60 (21.0), 50 (17.5), and 40% (14.0) (‰)) for 4 months. The growth performance was significantly increased at the temperature of 12 and 14 °C, and the feed efficiency was notably decreased at the temperature of 18 °C. The growth performance and feed efficiency were also significantly decreased at low salinity. The antioxidant responses such as superoxide dismutase and catalase were significantly increased by the high temperature and decreased by the low salinity. The immune responses such as lysozyme and phagocytosis were elevated by the temperature of 18 °C and decreased by the salinity of 50%. The results of this study indicate that the growth performance of juvenile sablefish, A. fimbria, is influenced by the temperature and salinity, and the excessive temperature and salinity levels can affect the antioxidant and immune responses.     

Influence of gamete density,salinity and temperature on the normal embryonic development of the mangrove oyster Crassostrea rhizophorae Guilding, 1828

Laboratory experiments were undertaken to determine the optimal environmental conditions and some of the other factors concerned in the development of Crassostrea rhizophorae embryos.Critical variables such as the number of spermatozoa per ovocyte during fertilization, the time of fertilization after gamete liberation, egg density, temperature and salinity were related to the proportion of normal D-larvae of C. rhizophorae in the resulting broods.The highest proportion of normal D-larvae was obtained at concentrations of 500–5000 spermatozoa/ovocyte, under conditions of 25‰ salinity at 25 ± 1°C. The optimal density of eggs, for the production of normal D-larvae, was 10⁴?4 × 10⁴ ovocytes/l. If fertilization was delayed for more than 45 min after liberation of spermatozoa the proportion of normal D-larvae was greatly reduced. The experiments demonstrated that the temperature for developing embryos should be below 30°C. At 20 and 25°C there was a high proportion of normal D-larvae 24 h after fertilization. The ideal salinity for embryonic development in C. rhizophorae was 25–37‰. Below a salinity of 16‰, less than 2.5% of the D-larvae were normal.     

Performance of Albino and Normal Channel Catfish (Ictalurus punctatus) in Different Water Temperatures

Experiments to determine effects of skin colour on growth in different water temperatures were conducted with albino and normally pigmented channel catfish (Ictalurus punctatus). In Experiment 1, fish were reared in 29°C, 27°C, or 22°C water from hatching to 40 weeks of age. Two additional groups were initially reared in 27°C water for either 12 or 16 weeks and in 22°C water for either the remaining 28 or 24 weeks, respectively. Results indicated that: (1) albino fish reared in 29°C water were superior in growth to normal fish; (2) albino fish did not differ from normal fish in growth when reared in either 27°C or 22°C water; (3) albino fish were inferior in growth to normal fish when reared in 27°C water for either 12 or 16 weeks of the 40-week growth period; (4) genetic differences between families and the age of fish strongly influenced relative growth of both colour types; and (5) both albino and normal catfish were able to compensate in growth at a later age. Experiment 2 began when fish were 48 weeks old and included both albino and normal fish which were either full-siblings or not genetically related. Results indicated that: (1) albinos and normal catfish did not respond differently to a 5°C water temperature change; (2) genetically related and randomly selected albino and normal fish produced consistent results concerning water temperature, skin pigmentation, and sex effects on growth; (3) increasing water temperature from 22°C to 27°C for 19 weeks resulted in increased weight and length of the fish by 144 and 80%, respectively; and (4) growth increase during the first 19-week period resulting from the higher water temperature was followed by a significant increase in the subsequent 10-week growth when the fish were in the lower water temperature.     

Effects of different salinity and temperatures on the growth,survival, haematocrit and blood biochemistry of Goldfish (Carassius auratus)

The aim of the present study was to investigate the effects of different salinities (0‰, 6‰ and 12‰) and temperatures (23, 27 and 31 °C) on the food consumption, growth, blood biochemistry and haematocrit of Goldfish. After 45 days of exposure to different salinities and temperatures, Goldfish showed a good adaptation to these salinities and temperatures in terms of blood biochemistry (glucose and triglyceride) and haematocrit. Salinities (0‰ and 6‰) and temperatures (23 and 27 °C) did not affect the weight gain, specific growth rate, final biomass and feed conversion rates, but these parameters were significant (P<0.05) at 12‰ salinity and 31 °C temperature. Plasma total protein levels decreased with the increase in salinity (P<0.05), while they were independent of temperature. In conclusion, Carassius auratus, a freshwater stenohaline fish, showed good growth in saline waters with maximum 12‰ salinity and 31 °C temperature.     

Effects of salinity and temperature on the growth and survival of New Zealand shortfin,Anguilla australis,and longfin,A. dieffenbachii,glass eels

The ideal water conditions for maximizing the performance of the nursery culture of glass eels harvested from the wild for aquaculture need to be determined for the New Zealand shortfin (Anguilla australis) and longfin (Anguilla dieffenbachii) eels. This study determined the survival and growth of glass eels reared under different temperature and salinity conditions in the laboratory. The growth and survival of shortfin and longfin glass eels reared in salt water (35‰) maintained at 25 °C was examined over 84 days from capture. The mean specific growth rate (SGR) was higher in shortfin [2.30±0.29% body weight (b.w.) day^?1] than longfin glass eels (1.52±0.06% b.w. day^?1), and survival was also higher in shortfin (76.0±4.16%) than for longfin glass eels (28.7±6.36%). A second experiment identified the effect of salinity (0, 17.5‰ and 35‰) and temperature (17.5 and 26.5 °C) on the acclimation, growth performance and survival of shortfin and longfin glass eels over a period of 84 days from capture. There was no incidence of mortality for either shortfin or longfin glass eels reared across all salinity treatments (0‰, 17.5‰ and 35‰) at 26.5 °C, while survival of shortfin and longfin glass eels reared at 17.5 °C was the highest in 17.5‰, followed by 35‰ and 0‰ treatments. Both temperature and salinity affected the SGR of shortfin glass eels, with the highest SGR observed for shortfin glass eels reared in 0‰ water maintained at 26.5 °C. In longfin glass eels, salinity alone had an effect on the SGR, with the highest SGR observed in glass eels reared in 0‰ water regardless of the water temperature (17.5 and 26.5 °C). In addition, the adaptability of glass eels to salinity was evaluated from the development and the physiological responses of gill chloride cell (CC) morphology. The number and size of CCs increased significantly with increasing salinity in both shortfin and longfin eels.     

The effects of temperature on respiration of Amur sturgeon,Acipenser schrenckii,at two acclimation temperatures

In order to clarify the respiratory responses strategy of Amur sturgeon Acipenser schrenckii exposed to water temperature changes, respiratory parameters of the fish were studied under two temperature regimes: fish acclimated at 13°C for Group I, temperature was increased to 16°C, 19°C, 22°C and 25°C and then returned stepwise to 22°C, 19°C, 16°C and 13°C; and fish acclimated at 25°C for Group II, the water temperature was reduced in steps to 22°C, 19°C, 16°C and 13°C, subsequently, returned to 16°C, 19°C, 22°C and 25°C. The results showed that the respiratory frequency (f_R), oxygen consumption rate (VO₂) and gill ventilation (V_G) of the fish were directly dependent on the acute temperature in both acclimation groups (p < .05). The initial 25°C VO₂ in Group II was significantly higher than the initial 13°C VO₂ in Group I (p < .05), but was significantly lower than that at 25°C in Group I (p < .05). In Group I, respiratory stroke volume (V_S.R) of fish significantly increased or decreased with the acute temperature increases or decreases, respectively (p < .05); oxygen consumption efficiencies (EO₂) of fish did not significantly show differences when temperature increased to 25°C from 13°C (p > .05), but the EO₂ significantly declined while returning to acclimation temperature (p < .05). In Group II, the V_S.R of the fish did not significantly change with acute temperature fluctuations between 25 and 13°C (p > .05), while the EO₂ increased with acute temperature increases (p < .05). The Q₁₀ values for f_R, VO₂, V_S.R, V_G and EO₂ were 1.53–1.72, 1.92–2.06, 1.07–1.60, 1.78–2.44 and 1.11–1.65 at 13–25°C of temperature interval respectively. Amur sturgeon showed partial metabolic compensation to temperature changes. The study results suggest that the ability of Amur sturgeon to regulate metabolism in response to acute temperature changes makes this species good adaptability in the aquaculture rearing.     

Cold tolerance of the banana prawn Penaeus merguiensis de Man and its growth at different temperatures

Critical thermal minima (CTMin) were determined for subadults of Penaeus merguiensis acclimated at 15, 18, 21 and 24 °C for 3 weeks. The effect of cooling rate on CTMin was also investigated. The CTMin of prawns from these respective acclimation groups were 5.3, 6.0, 7.4, 9.0 and 4.7, 5.4, 6.0, 7.3 °C at the cooling rate of 1 °C h^?1 and 3 °C h^?1 respectively. Both acclimation temperature and cooling rate had a significant effect (P < 0.01) on the CTMin. Observation during the acclimation period showed that the growth rate of prawns acclimated at 24 °C was much higher than those acclimated at 21, 18 and 15 °C. The results indicate that P. merguiensis can overwinter in aquaculture ponds in south‐east Queensland, Australia, or other subtropical areas and attain good growth if simple overwintering facilities are available.     

Influence of temperature and salinity on survival of the freshwater mullet, Rhinomugil corsula (Hamilton)

The upper incipient lethal temperatures of the freshwater mullet, Rhinomugil corsula, acclimated to 15, 20, 25, 30 and 35°C in fresh water, were 32.4, 34.1, 36.0, 36.2 and 36.5°C respectively, and the corresponding lower lethal temperatures were 10.5, 11.5, 13.2, 15.8 and 19.5°C. The mullet has a total tolerance (area of thermal polygon) of 569°C with an upper and lower thermal tolerance of 253 and 316°C². Likewise, the total resistance of the mullet was 391°C², with upper and lower resistance zones of 181 and 210°C respectively. The upper critical temperatures of swimming inhibition of R. corsula (17.2 cm; acclimation 30°C), determined in a swimming tunnel, were 35.2, 34.6 and 34.2 for water current velocities of 38, 62 and 77 cm s^?1 respectively. The corresponding lower critical temperatures were 26.2, 27.5 and 28.1°C. These results indicated the stenothermal nature of the mullet by comparison with other fishes, e.g. Tilapia mossambica.In tests on the influence of ambient salinity on thermal resistance, R. corsula survived longest at 7‰ (iso-osmotic salinity). At salinities above and below this point, survival times were shorter at any lethal temperature. In a tentative scheme for quantification of stress due to temperature and salinity at death (after acclimation to 30°C and tested at 37°C), the hypo-osmotic and hyper-osmotic stress were estimated to be 50 and 31% of the thermal stress (100%) respectively.     

Observations on salinity tolerance and osmoregulation in laboratory-reared Macrobrachium rosenbergii post-larvae (Crustacea: Caridea)

The tolerance of postlarval Macrobrachium rosenbergii to gradual and rapid increases in salinity was determined. Mortalities occurred at salinities around 25‰ and increased rapidly at levels ≥30‰ in both cases. However, acclimation substantially increased survival time at 35‰.Freezing point depressions of blood were measured from laboratory-reared M. rosenbergii postlarvae and juveniles exposed to various salinities from fresh water to approximately 35‰. The blood concentration was hyperosmotic to the medium at salinities from fresh water to about 17–18‰ and hypoosmotic at higher salinities. Postlarvae maintained a nearly constant blood concentration (freezing point depression = ?0.89 ± 0.13°C) over a wide range of external salinities (fresh water to about 27–30‰). The animals' osmoregulatory mechanisms failed at salinities ≥30‰, and thereafter the blood concentration paralleled that of the medium. The blood concentrations of juvenile shrimp grown for 5 months at salinities from fresh water to about 15‰ (freezing point depression = ?0.88 ± 0.07°C) closely resembled those of postlarvae.The osmoregulatory performance of young M. rosenbergii is generally similar to that of other brackishwater animals, but in their ability to hyperosmoregulate effectively in fresh water they more closely resemble fresh water species. It is suggested that M. rosenbergii may be able to conserve salt in dilute media by producing blood-hypoosmotic urine.An interesting stress symptom often preceded death of postlarvae in high salinities. The animals changed gradually from nearly transparent to opaque white and then died, usually within a day or so.     

Effects of stocking density,ration and temperature on growth,survival and metamorphosis of auricularia larvae of the California sea cucumber,Parastichopus californicus

The combined effects of stocking density (0.2, 0.5, 1, 2, 4 and 8 inds/ml) and dietary microalgal ration (20,000 and 40,000 cells/ml) and the sole effect of temperature (10, 12, 14, 16, 18 and 22°C) on the specific growth rate (SGR), per cent survival and per cent metamorphosis of auricularia larvae of the California sea cucumber, Parastichopus californicus, were evaluated in two separate experiments. The SGR was not significantly affected by stocking densities in the range of 0.2 to 4 inds/ml, but was significantly reduced at 8 inds/ml. The SGR of larvae fed 20,000 cells/ml was significantly reduced in comparison to those fed 40,000 cells/ml. Larvae had significantly higher per cent survival and per cent metamorphosis when reared at densities of 0.2 and 0.5 inds/ml compared with those reared at 2–8 inds/ml. Microalgal ration level did not significantly impact survival or metamorphosis. Larvae reared at 16 and 18°C had significantly higher SGRs and per cent metamorphosis than those held at all other temperatures, while per cent survival was highest at 16°C. Based on these results, we recommend rearing auricularia larvae of P. californicus at a stocking density at or below 0.5 inds/ml, a dietary ration of 40,000 cells/ml, and a temperature of 16°C.     

Dependence of Coregonus albula embryogenesis rate on the incubation temperature

Eggs stripped from Coregonus albula were incubated at different constant temperatures. The duration of embryogenesis varied from 183 days at 1.1°C to 45 days at 9.9°C. We describe the course of embryogenesis using 16 easily recognizable developmental stages (DS j). Development rate (DR j) for any given stage (DS j) is expressed as the reciprocal of time (in days) from fertilization to attainment of a given developmental stage. The generalized equation relating rate of development to stages DS j with respect to temperature (x) is

The values of DR to the “eyed egg” stage and to 50% hatch, expressed as percentage per day of the total development period, were computed and tabulated for temperatures ranging from 0.1 to 9.9°C. To verify the derived regression model we observed the course of embryogenesis of C. albula eggs incubated in a commercial hatchery at fluctuating temperatures. The observed times of attainment of the successive developmental stages were compared to the predicted times based on mean daily water temperatures. The time observed agreed well with times predicted by the model; the only exception was the time of hatching, which was systematically overestimated (5 to 7 days) by the model. This was possibly due to the influence of dissolved oxygen on the time course of hatching processes, which was not considered in our regression model.     

Growth of the hard clam Meretrix lusoria in Taiwan

The growth of hard clam Meretrix lusoria in Taiwan was observed as it was grown at six different stocking rates (55, 109, 172, 244, 344 and 455 clams/m²), from November 1979 to September 1980. It was found that the stocking rate had more effect on the increase of the total weight of the clam than on the shell length. Under the environmental condition of the study site, the optimum stocking rate was 244 clams/m². When the net production of the clam was lower than 1103 g/m², the growth of the individual clam was not affected by the stocking rate. But when the net production was higher than 1589 g/m², the growth of the individual clam seemed to be retarded. The average pH of the sea water in the clam culture area was between 7.9 and 8.4. The average dissolved oxygen content was between 4.2 and 11.2 mg/l. And the average water salinity was between 30‰ and 35‰. All these three factors did not seem to have direct relationship with the growth of the clams. Sea water temperature, however, showed great effects on the growth of the clams. It is shown that the growth of the hard clam was slow at a water temperature of 15–18°C, increased at 20–22°C, and accelerated at 25–32°C.     

Cortisol Response of Nile Tilapia,Oreochromis niloticus (L.), to Temperature Changes

Abstract

Nile tilapia, Oreochromis niloticus (L.), is a commercially-valuable fish species with high nutritional value. As a result of the intensive aquaculture of this species, handling, transport, and environmental changes that causes stress on these fish are unavoidable. The objective of this study was to determine the effects of gradual and acute temperature changes on juvenile tilapia. No significant difference (P > 0.05) was found among serum cortisol levels in juvenile tilapia when the water temperature was gradually increased from 27°C to 32°C, or 40°C, and maintained for 1 hour, although the levels were five times pretreat-ment levels. When tilapia acclimated to 27°C were subjected to 18°, 27°, 30°, 32°, 34°, 36°, 38°, or 40°C water for 10 minutes in a water bath, followed by a recovery period of 10 minutes at 27°C in the original aquaria, no significant difference (P > 0.05) in cortisol levels was observed among treatments except for significantly elevated levels at 38°C and 40°C. When tilapia acclimated to 27°C were subjected to the same temperature exposures but given a recovery period of 60 minutes at 27°C in the original aquaria, there was no significant (P > 0.05) increase in cortisol levels in tilapia among treatments from 18° to 36°C; but there was a significant (P > 0.05) increase between values from those treatments at 38° and 40°C. Acute temperature changes initiated the cortisol response as early as 10 minutes in fish following exposure to 38°C or 40°C and resulted in significant increases in the 38°C and 40°C treatments following 1 hour of recovery at 27°C. These results have implications for the management of tilapia during bacterial challenge, vaccination, and handling and transport during aquacultural activities.