沙地柏硬枝扦插繁育试验研究

本试验设置生长调节剂、扦插基质、枝条不同部位穗条3个处理因子,通过统计不同处理插穗的生根率、平均生根数和平均根长,研究各处理对沙地柏硬枝扦插繁育生根的影响。试验结果表明:不同生长调节剂、不同基质、不同部位穗条对插穗生根影响达显著水平,用0.6%GBT处理穗条生根率最高;以河沙为基质,插穗生根效果最好;枝条中部穗条扦插生根效果最佳。沙地柏扦插最佳的处理组合是枝条中部穗条用0.6%GBT处理,在河沙基质中进行扦插。     

一品红扦插繁殖研究初报

以一品红枝条为材料,笔者探讨了在温室条件下,不同扦插基质、扦插季节、部位穗条对扦插生根率等的影响。结果表明：于细沙中进行扦插,插条生根率可达87．2％;上部枝条扦插生根率比中部枝条扦插高16．4％;春夏季扦插生根率比秋冬季高23．2％,插后生根时间缩短了8d。     

常绿白蜡扦插繁殖技术

以当年生和2年生枝条为扦插材料,用不同处理方法对常绿白蜡进行扦插育苗试验,考察影响常绿白蜡扦插生根的主要因子,结果发现激素种类为影响常绿白蜡嫩枝扦插生根主要因素,其次是插穗类型和浸泡时间,激素浓度影响较小。试验分析表明当年生枝条扦插生根效果比2年生枝条扦插生根效果更好。常绿白蜡嫩枝扦插试验最佳处理为取当年生枝条中部用浓度为500mg／kg的ABT生根粉1号溶液快浸10s,扦插后60天生根率达60％。     

常绿白蜡扦插繁殖技术

以当年生和2年生枝条为扦插材料,用不同处理方法对常绿白蜡进行扦插育苗试验,考察影响常绿白蜡扦插生根的主要因子,结果发现激素种类为影响常绿白蜡嫩枝扦插生根主要因素,其次是插穗类型和浸泡时间,激素浓度影响较小。试验分析表明当年生枝条扦插生根效果比2年生枝条扦插生根效果更好。常绿白蜡嫩枝扦插试验最佳处理为取当年生枝条中部用浓度为500 mg/kg的ABT生根粉1号溶液快浸10 s,扦插后60天生根率达60%。     

美丽梧桐扦插青苗技术研究

以美丽梧桐萌芽枝条为材料,通过不同激素、不同浓度、不同育苗基质等扦插技术研究,结果表明:激素催根效果明显,NAA100mg/l处理枝条2h扦插生根率达93.33％;半木质化枝条远离顶芽部分扦插生根率高,达95.56％;沙床扦插生根率高,达82.22％;混合基质直接入袋扦插生根率为72.22％,减少了育苗程序,降低育苗成...     

鸡血藤扦插繁育技术研究

本文以广东梅州野生及人工种植的多年生鸡血藤Spatholobus suberectus为材料,以提高鸡血藤扦插育苗繁殖系数为目的,对影响鸡血藤扦插生根的主要因素开展扦插试验。结果表明:人工植株和野生植株的穗条扦插生根率无显著差异;扦插基质类型对生根率有显著影响,采用黄心土作为基质生根效果最好;1~3a生枝条中,鸡血藤的木质化枝条生根率要高于半木质化嫩枝,粗度>0.8cm的木质化枝条生根率最高;外源激素种类及其质量体积浓度均对扦插生根率产生显著影响,采用质量体积浓度为500mg·L^-1的GGR浸泡插穗基部1h扦插生根效果最好;不同季节扦插对比试验显示春季扦插的生根率要显著高于其他季节;扦插方式试验表明营养袋扦插生根率与插床扦插无显著差异。     

丹桂扦插繁殖技术研究

研究不同扦插基质、不同NAA浓度和不同扦插季节及不同部位枝条对丹桂扦插繁殖的影响,结果表明:在夏季用山地红壤以及200 mg.L-1NAA处理穗条最有利于提高扦插生根率;扦插基质、NAA浓度和季节对丹桂扦插生根数量影响不显著;NAA处理对穗条新梢的生长有促进作用;不同部位枝条对于扦插生根率影响甚小。     

小紫珠硬枝扦插试验研究

按不同年龄、不同直径和不同激素处理对泰山野生小紫珠进行硬枝扦插试验，结果表明：在不采用任何激素处理的情况下，1年生枝条的插穗生根率达48．6％，明显优于多年生枝条；在1年生枝条中，又以直径0．6～0．8cm的插穗为宜，其生根率为67．3％。采用激素处理普遍可以提高扦插生根率，其中以1000mg／L的ABT1号生根粉浸泡插条基部10秒钟的处理效果最好，生根率达95．0％，为清水对照的185．2％。     

不同基质与激素对柳杉扦插繁殖的影响

以柳杉(Cryptomeria fortunei)枝条作枝条为插穗材料,研究了不同基质、不同水杨酸浓度和生根剂种类、浓度以及浸泡时间对扦插的影响。通过对保存率、生根率、不定根数的分析表明:蛭石∶珍珠岩∶河沙(1∶2∶2)基质对柳杉扦插的效果好于黄心土∶珍珠岩∶河沙(1∶2∶2),以及珍珠岩∶河沙(1∶1)及河沙的基质,生根率为48%;不同的生根剂种类、浓度和浸泡时间对柳杉扦插繁殖影响显著,其中以200 mg/L ABT1号处理1 h效果最好,生根率为46.8%。在高温季节用200 mg/L的ABT1号生根剂浸泡0.5 h,再用水杨酸浸泡0.5 h,能够明显提高柳杉扦插生根率,生根率达80%。     

油料植物南美油藤扦插技术研究

以2 a生南美油藤枝条作为穗条研究不同生根剂种类、生根剂浓度、穗条根部生根剂浸泡时间、穗条不同木质化程度以及以0.5年生枝条作为穗条研究不同品种对南美油藤扦插生根效果的影响。结果发现:南美油藤扦插7 d左右出现愈伤组织,10左右愈伤组织出出现白芽点,12 d左右开始生根,20 d可以移栽。不同生根剂种类、生根剂浓度、穗条根部生根剂浸泡时间对南美油藤扦插生根率具有显著影响,生根剂IBA,浓度500~1 000 mg/L,浸泡时间20~60 s对南美油藤扦插生根效果最优;木质化程度低的枝条作为穗条扦插效果优于完全木质化枝条,已经驯化的南美油藤V扦插效果优于其他几个品种。     

‘大10’果桑全光照弥雾嫩枝扦插试验

研究了GGR-6对‘大10’果桑全光照弥雾嫩枝扦插的影响。结果表明:GGR-6处理能够显著(P<0.05)提高‘大10’果桑插条生根率,明显缩短生根时间;GGR-6 100 mg.kg-1处理生根效果最好,插条的生根率、平均生根数和平均根长分别较对照增加50.0%、110.6%、68.1%,生根时间缩短10 d。     

杉木优良无性系组培快繁与容器苗培育技术研究

以20年生杉木优良无性系根部萌条获得的无菌组培苗作为试验材料,在前人研究基础上,进一步对杉木组培体系进行优化,并开展了指数施肥对组培苗轻基质容器苗生长影响研究,结果表明:最适培养基为DCR+6-BA0.4 mg·L-1+NAA0.02 mg.L-1,其增殖倍数达到4.19,每瓶平均总芽数高达31个,去除顶芽接种方式较之带顶芽,其增殖倍数、有效芽所占百分比分别提高了21.7％ ～ 45.5％和31.7％ ～ 39.2％;最佳生根培养基为1/4DCR+NAA0.8 mg.L-1,生根率达76.4％,平均生根条数为6.67;不同生根方式对移栽成活率皆无明显差异,均达到90％以上;指数施肥对苗高和高径比影响差异显著,N150为最优.     

母株年龄、激素种类及其浓度对日本落叶松扦插生根的影响

系统地研究了全光雾插条件下母株年龄、激素种类及其浓度对日本落叶松半木质化插穗生根的影响,并在此基础上利用隶属函数法对各处理的生根效果进行了综合评价,分别为不同年龄阶段的落叶松扦插选配出最佳激素种类及其浓度的处理组合,12.5、8.5 a子代林插穗经最佳组合处理后生根率分别可达78.8%、92.5%,极大地提高了较高年龄日本落叶松扦插繁殖的生根能力.三因素方差分析结果表明,母株年龄(A)、激素种类(B)和激素浓度(C)对落叶松插穗生根率、生根量和偏根率等生根性状的影响达极显著或显著水平,同时三因素互作效应(A×B×C)及双因素互作效应(A×B、A×C、B×C)对绝大多数性状的影响也很显著.根据分年龄阶段进行的激素种类、浓度及其互作效应的比较结果,可以看出处理间生根性状的变化缺乏明显的规律性.     

Vegetative propagation of Colutea arborescens L., a multipurpose leguminous shrub of semiarid climates

The vegetative propagation of the multipurpose leguminous shrub Colutea arborescens from leafless hardwood cuttings was examined. Specific attention was paid to the factors: 1) cutting origin (apical, medial or basal); 2) indole-3-butyric acid (IBA) treatment (0, 50 or 200 ppm); and 3) relative humidity of the propagation environment (high or low humidity). After 120 days, 67.6% of the C. arborescens cuttings had rooted. The rooting ability of the cuttings was significantly influenced by the cutting origin and hormonal treatment. No effects on rooting percentage were shown by the relative humidity of the propagation environment. The best rooting response (95%) was shown by basal cuttings treated with 200 ppm IBA. Cutting origin, IBA treatment and relative humidity were significantly related to the root number, longest root length and the fresh/dry biomass of roots and leaves. Under conditions of high humidity, basal cuttings treated with 200 ppm IBA gave rise to the most highly developed plants. The positive relationship between sprouting and rooting suggests that successful propagation is related to the shoot formation potential of the cutting's food reserves. These findings were used to propose an efficient method for the vegetative propagation of C. arborescens plants. This revised version was published online in June 2006 with corrections to the Cover Date.     

闽楠扦插繁殖技术研究

通过正交试验设计研究不同年龄母株穗条、扦插基质、生根促进剂浓度、扦插时间对闽楠生根率及根生长的影响。结果表明:扦插基质、母株年龄、扦插时间对闽楠生根率及根生长影响显著,不同生根促进剂浓度对生根率及根生长无显著影响;各因素的最佳组合为:1年生母株穗条经ABT1#800 mg.L-1速沾后扦插于砻糠灰中;3月份扦插生根率最高,达89.6%。     

Distinct effects of auxin and light on adventitious root development in Eucalyptus saligna and Eucalyptus globulus

Adventitious rooting is essential for vegetative propagation of woody species. We studied the effects of auxin and light on the development of adventitious roots in cuttings obtained from seedlings of Eucalyptus saligna Smith and E. globulus Labill in an attempt to characterize the adventitious rooting process and identify factors controlling rhizogenesis. Root development was scored as rooting percentage, root density (roots per rooted cutting), mean rooting time and root length. In both species, rooting time was reduced in the presence of auxin. Cuttings from 2-month-old E. saligna seedlings were responsive to lower auxin concentrations than comparable cuttings from E. globulus seedlings. Cuttings from 3-month-old E. saligna seedlings rooted promptly and rooting was not significantly affected by light conditions. In contrast, rooting of cuttings from 3-month-old E. globulus seedlings exhibited recalcitrant behavior and no roots were formed if illuminated during the root formation phase. Effective root regeneration of E. globulus cuttings was obtained by a 4-day exposure to 10 mg l(-1) IBA and culture in darkness during the root formation step. Loss of rooting capacity with seedling age was more pronounced in E. globulus than in E. saligna. The possibility of switching adventitious rooting off and on by manipulating light regime and exogenous auxin supply in E. globulus, and the constitutive nature of rooting in E. saligna may provide useful models for examining the rooting process at the biochemical and molecular levels in Eucalyptus.     

Cutting propagation of <Emphasis Type="Italic">Santalum austrocaledonicum</Emphasis>: the effect of genotype,cutting source,cutting size,propagation medium,IBA and irradiance

Developing methods for routine clonal propagation of sandalwood (Santalum austrocaledonicum) is important for its domestication and development as a commercial agroforestry species. The amenability of this species to propagation by leafy stem cuttings in low-cost non-mist propagators was assessed in four separate experiments. These experiments evaluated the effects of (1) genotype (15 genotypes from two island provenances), (2) cutting position on the stock plant (apical, medial and basal), (3) cutting size (1-node/400 mm² and 2-node/800 mm² leaf area), (4) three propagation media [scoria (5 mm, air-filled porosity (AFP)—29%), vermiculite and perlite (1:1 v/v, AFP—46%) and vermiculite, perlite and peat (2:2:1 v/v/v, AFP—42%)], (5) indole-3-butyric (IBA) (3000, 4000 and 8000 ppm) and (6) irradiance in the propagator [daily light integral (DLI) 5.3, 3.9, 2.6, 2.2 mol m^?2 day^?1]. IBA, propagation media and cutting size had no significant effect on rooting percentage, root number or root growth. Evidence of provenance-based variation in rooting capacity was recorded with greater rooting success for genotypes from the island of Erromango compared with Tanna. Variation in adventitious root induction was also recorded between individual genotypes from Erromango across all four experiments. Cuttings collected from the apical and medial parts of the shoot on the stock plant had higher rooting percentage than those collected from the base. Differences in rooting capacity between apical and medial shoots were variable between experiments and may be attributed to different levels of hardening. The use of artificial shade (~?70%) to achieve a mean DLI of between 2.6 and 3.9 mol m^?2 day^?1 increased rooting percentage compared to both lower (2.2 mol m^?2 day^?1 or?~?90% shade) and higher (5.3 mol m^?2 day^?1 or?~?50% shade) irradiance treatments. Leaf retention of cuttings in the propagator was positively associated with the percentage of cuttings with adventitious roots, with highest percentage in cuttings with full leaf retention, regardless of original cutting size. This study demonstrated S. austrocaledonicum seedlings can be successfully propagated by cuttings provided the propagation conditions are optimized for each genotype.     

Key roles of leaves, stockplant age, and auxin concentration in vegetative propagation of two African mahoganies: Khaya anthotheca Welw. and Khaya ivorensis A. Chev

Leaf area, IBA concentration and age of stockplants were all found to be important factors for successful rooting for vegetative propagation using single-node cuttings of the two main African mahogany species: Khaya anthotheca and K. ivorensis. Cuttings with leaf area of 30–50 cm² had the best rooting percentage and cuttings with about 30 cm² had the most number of roots per cutting in K. anthotheca. Khaya ivorensis, cuttings with 10–30 cm² leaf area had the highest rooting percentage. Cuttings collected from 1-year-old stockplants recorded the highest rooting percentage and largest number of roots per cutting. Cuttings from 3-year-old stockplant of the same seeds sources had the lowest rooting suggesting aging negatively impacted rooting ability. The effect of auxin concentration, on rootability was examined with cuttings of K. anthotheca. An IBA concentration of 0.8% was the best exogenous auxin concentration for percentage rooting, number of roots per cutting and the length of the longest root per cutting.     

Improving regeneration of mahogany: techniques for vegetative propagation of four African mahogany species using leafy stem cuttings

Regeneration to ensure sustainable production and conservation of mahogany species globally is considered very important to foresters, investors in plantation, conservationists and timber industries worldwide. Natural regeneration of the mahoganies has been found to be far less than the rate of exploitation hence strong efforts are being made to develop other methods to regenerate mahogany seedlings to augment the low natural replacement and to ensure sustainability. This study was commissioned to investigate the effect of rooting media (river-sand, top soil and a mix of the two (50:50 v/v) on the rooting ability of four mahogany species (Khaya grandifoliola, K. ivorensis, Entandrophragma angolense and E. utile) using non-mist propagators. We investigated the effects of the source of stem cutting within a shoot (top, middle and basal position) on the rooting ability of stem cuttings from the four species in river-sand. Roots per cutting, longest root length and rooting percentage differed significantly among species in all three rooting media. Rooting ability was greatest in K. grandifoliola, which exhibited the highest average rooting percentage (73 %), longest average single root (9.9 mm) and the most roots per cutting (4.9). Rooting percentage was higher in the Khaya species than in the Entandrophragma species in all three rooting media. The mixed medium had the highest rooting percentage and the highest number of roots per rooted cutting in the two Khaya species, while the topsoil media had the best rooting percentage in the Entandrophragma species. Rooting of the two Khaya species can be maximized using a mixture of river-sand and topsoil. Cutting position within a shoot had significant impact on rooting in all four species with rooting percentage being best from middle positions in all four species. However, length of longest root and the number of roots per rooted cutting varied between the middle and basal portions. The study provides useful information for vegetative propagation of leafy stem cutting of the four African mahogany species, which could contribute to regeneration and conservation of these important timber species in the tropics.     

大丽花优良品种扦插繁殖与栽培技术研究

以大丽花品种大金红和粉西施为试材,利用不同生长调节剂（50mg／L萘乙酸（NAA）、50mg／L吲哚乙酸（IBA）和50mg／L生根粉（ABT））对大丽花插条进行处理,以清水为对照,结果表明,50mg／L NAA处理的大金红幼芽生根数、根长和生根率最高,生根时间最短。50mg／L NAA处理粉西施插条以10s生根效果最佳;研究了大丽花的块根保存、扦插繁殖方法以及露地栽培管理技术,以期为生产上大规模繁育大丽花优良品种提供理论依据,满足园林绿化美化对大丽花优质种苗的需求。