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新疆部分地区牛新孢子虫病的血清学调查 总被引:7,自引:0,他引:7
应用间接免疫荧光抗体试验,酶联免疫吸附试验,斑点酶联免疫吸附试验3种检测方法,对新疆地区部分牛和牦牛的血清样品进行新子虫病抗体检测。结果显示,298头份牛血清全部为阴性,4份牦牛血清1份为阳性。 相似文献
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为初步了解湖南及周边地区猪伪狂犬病免疫及野毒感染情况,于2020年1—12月从湖南及周边地区238个猪场采集血清样品7194份,通过酶联免疫吸附试验(ELISA)分别检测伪狂犬病病毒(PRV)gB抗体和gE抗体.结果发现,667份血清样品为PRV-gE抗体阳性,5937份血清样品为PRV-gB抗体阳性,其平均阳性率分别... 相似文献
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鸡传染性法氏囊病快速诊断方法的研究 总被引:6,自引:0,他引:6
本文报道了一种既可以检测IBD囊毒抗原,又可以检测IBD抗体的酶联免疫吸附试验(ELISA)双抗体夹心法。用本方法检测IBD阳性法氏囊样品122份,结果检出阳性120份,符合率为98.4%。检测阴性法氏羹样品88份,均为阴性。本方法与琼脂扩散试验方法比较,共检测人工感染发病的病死鸡法氏囊97份,结果琼脂扩散检出阳性法氏囊49份(50.51%),阴性48份(49.48%),而用本方法检测结果,全为阳性,检出阳性率为100%,比AGP法检出率高49.48%。检测高免蛋黄液的抗体。16个样品(以2倍递增进行稀释),结果本方法比琼脂扩散试验高1~2个滴度。试验证明该方法特异、敏感,并且快速简便,试验在室温条件下进行,不需要任何仪器设备,用肉眼观察颜色的变化就可判断试验结果,试验反应时间仅需10分钟左右。 相似文献
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用酶联免疫吸附试验(ELISA)对2012年上海市某地区27个养殖场户1 760份猪血清样品进行了猪流感(H3N2)血清学抗体检测。检测结果表明,在27个养殖场户中均检出H3N2抗体阳性,共检出阳性320份,阳性率为18.18%。 相似文献
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上海地区奶牛犬新孢子虫病血清学抗体检测 总被引:2,自引:0,他引:2
为了掌握上海地区奶牛新孢子虫病的流行情况,应用酶联免疫吸附试验对上海地区奶牛血清样品进行新孢子虫病抗体检测。结果表明,被检的41个奶牛场1239份奶牛血清中有63份抗体阳性,阳性率为5.08%。各场阳性率变幅较大(0-44.23%)。 相似文献
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美国明尼苏达州立大学的研究人员在2010年7~8月对泰国放牧鸭的禽流感病毒暴露风险进行了相关研究。他们采集了6254份鸭血清,采用酶联免疫吸附试验(ELISA)方法检测了针对A型流感病毒的核蛋白(NP)和H5亚型血凝素的抗体。检测结果显示,85%的血清样品为A型流感病毒抗体阳性,对NP阳性的样品再次进行H5亚型血凝素抗体检测,39%的样品为阳性,4%为疑似阳性。而这些阳性或疑似阳性的样品12%的血凝效价大于或等于1∶20。研究结果证实A型流感血清阳性的风险因素包括鸭日龄、家禽间的接触、 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献
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以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献