中国育成的梨品种（系）系谱分析

为全面了解中国梨的育种成果,系统收集并分析了1949—2018年中国报道育成的327个梨品种（含品系,下同）,并绘制了以主要品种为亲本育成品种的系谱关系图。育成的梨品种中,杂交品种最多（207个,63.30%）,其他依次是芽变品种（70个,20.41%）、实生选育品种（41个,12.54%）和诱变品种（9个,2.75%）。根据品种特性分类,育成品种中抗黑星病品种最多（134个,占40.98%）,其次是抗寒品种（91个,27.83%）和耐贮品种（84个,25.69%）。果实成熟期明确的265个品种中,早熟、中熟和晚熟品种分别占25.28%、43.77%和30.95%。分析亲本来源明确的301个品种,发现这些品种由137个亲本育成,包括49个国内地方品种,44个国外引进品种和44个衍生品种,其中‘苹果梨’的衍生品种最多（66个）,其次为‘二十世纪’（57个）、‘鸭梨’（43个）、‘茌梨’（36个）、‘砀山酥梨’（21个）和‘库尔勒香梨’（14个）。通过系谱分析发现,不同地理来源或者植物学性状差异较大的亲本组合选育出的杂种后代具有较强的杂种优势。     

我国高类黄酮(红皮与红肉)苹果育种取得突破性进展

“医食同源,吃营养,吃健康”已经成为人们的共识。针对我国苹果产业品种单一、特色多样化品种严重不足、主栽品种’红富士'果实着色、风味品质及结果习性等性状需要进一步改良、苹果育种特别是红肉苹果育种周期长、缺乏优异种质等问题,系统介绍了以“高类黄酮(红皮与红肉)”为主线,以新疆红肉苹果挖掘与创新利用为研究重点,在品质形成与芽变机理、红肉苹果优异种质创制、高效育种技术创建、特色多样化新品种培育及其配套高效栽培技术创新等方面取得的最新研究进展及其成果,旨在为其他野生果树资源的评价挖掘与创新利用提供参考。     

Molecular Characterization of Some Apple Accessions Using DNA Markers Associated with Fruit Skin and Flesh Colour

Apple (Malus domestica Borkh.) is one of the most widely grown and economically important fruit crops in the world. Skin colour is one of the most important criteria for marketing of apple fruits. Thus, improving fruit colouring is one of the main objectives of apple breeding programs. Even though environmental conditions may affect the colouring of apple, understanding the genetic basis of colouring is important to accelerate the breeding process. In recent years, molecular biology and genetic studies were conducted to explore the molecular basis of colouring in apple. Many genes responsible for the anthocyanin synthesis were identified and their associations with colouring of apple fruit flesh and/or skin were demonstrated. In addition, some DNA markers associated with fruit flesh and skin colour have been developed for screening apple cultivars and hybrids. In this study, 90 apple accessions of international, national, and local decents were selected from the genetic resources collection. Genomic DNA was isolated from leaf tissue of all acession and screened with four different DNA markers associated with fruit flesh and skin colour. Apple accessions in the collection were characterized and their genotypes and genetic potential for fruit colouration were determined by different DNA markers. Additionally, the use of these DNA markers in different apple accessions, well-characterized cultivars and uncharacterized local and national types were investigated.

     

世界葡萄抗寒育种的成就与展望

近2个世纪,通过选种和杂交育种,世界葡萄抗寒育种在抗寒性与果实优质性状的结合方面取得了突破,育成许多抗寒性极强(耐-30～-40℃低温)、品质优良的鲜食、酿酒和制汁品种。由于葡萄的抗寒性是受多基因控制的数量性状,杂交后代的抗寒性基本呈以亲中值为峰顶的正态分布,所以我国应积极引进这些品种,在华北、西北等葡萄主产区试验、推广露地越冬栽培的同时,利用它们与现有抗寒性较弱的主栽品种(抗寒性-15～-18℃)杂交,培育适合我国冬季气候条件的抗寒品种(抗寒性在-25℃左右),迅速提高我国葡萄抗寒育种水平。     

Effects of 1-aminocyclopropane carboxylic acid on the rate of ethylene release from detached fruiting spurs and on fruit abscission in apple

Summary

1-Aminocyclopropane carboxylic acid (ACC) was applied to apple (Malus × domestica Borkh.) trees or to individual fruiting spurs between full bloom (FB) and 31 d after full bloom (DAFB; mean fruit diameter of 20 mm) to determine the effects of ACC on the ethylene released from detached spurs and on fruit abscission. The rate of ethylene release from detached fruiting spurs increased 1 d after applying 50 mg l^–1 ACC at full bloom or 16 DAFB (mean fruit diameter of 10 mm), but ACC had a reduced effect on the rate of ethylene release when it was applied 31 DAFB (mean fruit diameter of 20 mm). ACC reduced fruit set in ‘GoldRush’ apple in a concentration-dependent manner in 2009 and in 2010, but had no thinning activity on ‘Pink Lady’ in 2010. ACC at 200 mg l^–1 resulted in the yellowing and abscission of ‘GoldRush’ spur leaves in 2009, but not in 2010. The effects of combining ACC and 5 mg l^–1 naphthaleneacetic acid on fruit set in ‘GoldRush’ apple were additive. The application of 200 mg l^–1 ACC when fruit are ca. 10 mm in diameter may therefore be a useful chemical thinning treatment for apple fruit.     

Evaluating the effectiveness of different strategies for calcium application on the accumulation of calcium in apple (Malus × domestica Borkh. ‘Braeburn’) fruit

Summary

Orchards displaying calcium (Ca) deficiency are a common phenomenon worldwide, despite the presence of sufficient Ca in the soil and the plant. A 3-year trial was conducted between the 2007 – 2008 and 2009 – 2010 growing seasons to evaluate the contributions of soil and foliar Ca applications to Ca concentrations in ‘Braeburn’ apple (Malus × domestica Borkh.) fruit. Ca(NO₃)₂ (Calflo; Yara Africa, Fourways North, South Africa) was applied as six separate foliar sprays until run-off. Applications were made at 1-week intervals between approx. 21 – 70 d after full bloom (DAFB) at 6.75 ml l^–1. Soil applications of Ca (Tropicote^TM; International ASA, Oslo, Norway) at 300 kg ha^–1 were applied at fruit set, or after harvest, according to standard practice. Mineral analysis was conducted to assess the soluble Ca concentrations of whole fruit (without pips and stalks), to quantify the contribution of foliar sprays or soil-applied Ca. Fruit Ca concentrations were maintained at satisfactory levels (4.5 mg Ca 100 g^–1 FW) at harvest by applying a series of six foliar sprays early in the season (for all seasons) during the trial period. Fruit Ca concentrations at 80 DAFB were highest in the treatments with foliar applications of Ca. In 2009 – 2010, Ca concentrations in apple fruit were lowest (8.38 mg 100 g^–1 FW) for soil application of Ca at fruit set. Ca applications to soil after harvest in the previous season, and soil applications shortly after fruit set in the current season, did not significantly increase Ca concentrations in current-season fruit, providing soil Ca levels were above the minimum requirement for apple trees. A possible explanation is that apple trees regulate their uptake of Ca through the roots when soil Ca is available in sufficient quantities. This confirms the importance of active root growth for efficient Ca uptake by apple trees when applying Ca to the soil.     

The risks of success in quality vegetable markets: Possible genetic erosion in Marmande tomatoes (Solanum lycopersicum L.) and consumer dissatisfaction

Following consumer complaints about the quality of modern varieties of tomato, landraces have strengthened their quality markets. In Spain, two tomato landraces, ‘Montserrat’ and ‘Pera Girona’, are grown in contiguous areas and have different market niches. We used amplified fragment length polymorphism (AFLP) to characterize 13 accessions of Montserrat, 14 accessions of Pera Girona, and 4 control varieties. We found a narrow genetic base for ‘Montserrat’ and ‘Pera Girona’ (8.5% of polymorphic loci) and no differences between the landraces. We studied agronomical and sensory traits to determine why the two landraces continue to have separate market niches. We found high variability among accessions within each landrace and overlapping among accessions of both landraces for all traits except fruit morphology. Consumers probably came to associate the organoleptic quality of these landraces with their external traits, but due to spontaneous crossings and introgressions these relations have been lost. Selection within landraces will be necessary to reestablish the link between morphology and sensory value and to consolidate these quality markets.     

Harvest parameters to improve the storability of high quality ‘Reinette du Canada’ apple

Summary

‘Reinette du Canada’ apple (Malus domestica Borkh.), which has been declared a Protected Designation of Origin (PDO) product throughout the European Community, is severely affected by bitter pit during storage, but increasing demand for organic produce requires chemical post-harvest treatments to be replaced with organic practices. The objective of this work was to determine the optimum stage of fruit maturity to improve the storability of high quality ‘Reinette du Canada’ apple during storage without applying post-harvest treatments. Seasonal conditions influenced fruit quality and the occurrence of storage disorders in ‘Reinette du Canada’. Harvest maturity also influenced the quality of ‘Reinette du Canada’ apple during storage, with later harvests reducing the development of bitter pit and increasing the soluble solids content:titratable acidity (SSC:TA) ratio. This study suggests that the SSC:TA ratio was a useful parameter for determining the optimum harvest maturity for high acidity, ‘Reinette du Canada’ apple. High quality fruit were obtained after long-term storage when the SSC:TA ratio exceeded 11.0, and fruit firmness at harvest exceeded 90 N. If these thresholds were not achieved, the quality of harvested ‘Reinette du Canada’ apples may not be sufficient to market fruit with the PDO designation. The results of our study also suggested that skin hue angle (hº) measurements may provide a quick and useful index that could replace fruit firmness measurements in the future.     

Determination and confirmation of S-RNase genotypes of apple pollinators and cultivars

Summary

We analysed the S-RNase genotypes of 23 crab apple (Malus spp.) pollinators and 102 cultivars of domestic apple (Malus pumila Mill.) by PCR amplification and digestion. Within the 23 pollinators, four pollinators, ‘Hopa’, ‘Jack’, ‘Pink Perfection’ and ‘Profusion B’, each had two unidentified S-RNase alleles. These cultivars should be useful pollinators for all domestic cultivars. Twenty-one of the domestic cultivars exhibited S-genotypes contrary to those expected from their supposed parentage, suggesting that one or both reported parents were wrong. We confirmed many of the S-RNase genotypes by pollination tests.     

Influence of a pre-harvest reflective orchard mulching film on lipid peroxidation and anti-oxidant activity in apple fruit during post-harvest storage

Summary

Studies have shown that applying a reflective film to the floor of an orchard enhances fruit colouration. Here we assessed whether this practice also prolonged the post-harvest storage life of apple (Malus domestica Borkh cv. Fuji and Malus prunifolia [Willd.]) fruit. Strips of reflective mulching film (RMF) were placed beneath the apple trees and RMF-treated fruit were compared with those from untreated control (CK) trees. Enzymes involved in reducing lipid peroxidation such as superoxide dismutase (SOD) and catalase (CAT) are associated with delaying fruit senescence. Their activities, as well as malondialdehyde (MDA) concentrations, were quantified as indicators of physiological changes related to lipid peroxidation and membrane damage in the peel and flesh of RMF-treated or control apple fruit during 50 d of post-harvest storage at 4ºC. SOD activities increased in the peel and flesh from RMF-treated fruit during the first 40 d post-harvest, but declined significantly during the final 10 d of storage. RMF treatment resulted in increases in CAT activities of 5 – 14% and 16 – 29% in the peel and flesh of RMF-treated apples, respectively, compared with untreated CK fruit. Conversely, MDA concentrations were 7 – 23% and 14 – 30% lower in the peel and flesh, respectively, of RMF-treated fruit than in CK fruit over the 50 d storage period. Similarly, RMF treatment resulted in reductions of 7 – 20% and 3 – 10% in saturated fatty acid (SFA) concentrations in the peel and flesh, respectively, compared to CK fruit, while the corresponding polyunsaturated fatty acid (PUFA) concentrations increased by 2 – 10% and 3 – 7%. In conclusion, the use of RMF in apple orchards appears to be an effective technique to delay post-harvest senescence in apple fruit.     

新种质创造支撑果品产业升级——红肉苹果和‘库尔勒香梨’种质资源利用以及‘红富士’芽变选种案例分析

种质资源评价挖掘和创新利用是现代农业的重要特征。新疆是苹果等多种落叶果树起源演化中心,种质资源极为丰富。笔者系统介绍了近几年来在新疆红肉苹果和‘红富士’以及‘库尔勒香梨’资源评价挖掘和创新利用方面的研究进展,主要包括红肉和红皮苹果育种理论与技术创新、新品种培育和配套技术研发以及优质、耐贮、晚熟梨新品种创制,为苹果和梨产业升级提供了重要支撑。     

Molecular diversity of Chinese Cucurbita moschata germplasm collections detected by AFLP markers

Cucurbita moschata is an important vegetable crop. Although a total of 1032 landraces of C. moschata are maintained in China, little is known about their genetic diversity. Molecular characterization is needed to facilitate the use of this C. moschata germplasm collection in breeding. Seventy-four Chinese accessions and 15 accessions from other countries were selected for evaluation based upon variation in fruit traits and geographical origin of molecular diversity with AFLP analysis. Nine pairs of EcoRI/MseI primers produced 500 fragments, of which 75.57% were polymorphic, indicating a high degree of diversity. The accessions from China were classified into two clusters, which were clearly differentiated from the accessions originating from Mexico, Guatemala, Honduras, and Ecuador. Chinese group genetically more closely related to other Asian countries group (India and Japan). In general, the accessions from the Americas had a greater number of unique loci than those from China. The differences are probably due to a limited number of introductions and genetic drift. The Americas are the center of origin of C. moschata and therefore more diverse. With AFLP analysis, the accessions did not clearly group according to fruit shape; however, sub-clusters exist in acorn- and dumbbell-shaped accessions. The assessment of genetic distance, along with some unique traits among the different genotypes, could be useful in further genetic studies and the selection of the most adequate accessions for use in breeding programs.     

Ascorbic acid level of Belgian apple genetic resources

With the help of the public, a wide collection of old apple cultivars (cvs) was established progressively at our Department from 1975 onwards. Currently, more than 1400 apple accessions are being maintained and three quarters of them are subjected to field evaluation for disease resistance and agronomic characters. This paper presents results of the ascorbic acid (Vitamin C) contents of old cvs combining interesting disease resistance and agronomic features in order to promote them as cvs adapted to production systems which are more environmentally friendly or to be used as parents in breeding programmes. First, a methodological study shows the importance of the variability of ascorbic acid content depending on fruit position in the trees, fruit size, fruit skin colour and maturity stage. Statistical analyses show that the sample size of six fruits per cv is sufficient to detect a difference of 20% between cvs. Old apple cvs such as Grenadier^RGF, Transparente de Croncels, Bramley’s Seedling, Reinette des Capucins, Reinette Libotte and Reinette de Waleffe contain three and seven times more ascorbic acid than the commercial cvs Gala and Elstar, respectively. The paper highlights the need to evaluate the rich potential in our genetic resources.     

Determination of optimal harvest boundaries for ‘Ambrosia’ apple fruit using a delta-absorbance meter

In this study, a new chlorophyll measurement tool, the delta absorbance (DA) meter, was used to develop an optimal harvest maturity model for ‘Ambrosia’ apple (Malus × domestica Borkh.) fruit. Fruit from four commercial orchards in the Annapolis Valley, Nova Scotia, Canada, were sampled (25 fruit from three or four trees per location) over nine consecutive weekly harvests during the 2011 and 2012 growing seasons, and 8 weeks in the 2013 season. At each harvest, five fruit from each orchard site had their index of absorbance difference (I_AD) values, firmness, mass, titratable acidity (TA), soluble solids content (SSC), red skin colouration and internal core ethylene concentrations measured. Following approx. 3 months of storage at 3.5°C, 20 fruit from each site were removed and assessed for the incidence of disorders such as senescent breakdown, cortical browning and coreflush. Chlorophyll concentrations in the epidermis were strongly and positively related to I_AD values in the same tissue (P ≤ 0.001), confirming the assumption that chlorophyll was the basis for the DA meter I_AD signal. In addition, I_AD values declined significantly during fruit maturity and were negatively related to harvest week (P ≤ 0.001). The optimum harvest period was identified by aligning all ‘at harvest’ I_AD values, fruit quality measurements, and ‘post-storage’ disorder data with the corresponding harvest week. I_AD values associated with harvests having the highest commercial fruit quality then delineated the optimal harvest boundaries. The upper boundary I_AD value of 0.47 was defined as ‘when to begin harvest’, while the lower boundary I_AD value of 0.28 was considered to be ‘when to end harvest’ for long-term storage. The use of a DA meter and its I_AD value to define the optimal harvest boundaries may be applicable to all commercial apple cultivars, but should be developed for each cultivar and growing region.     

Comparison of the compatibility and metaxenia effects of several dessert apple and ornamental Malus cultivars with Cox’s orange pippin

Flowers of apple cv Cox’s Orange Pippin were hand-pollinated with diluted or undiluted pollen of six dessert and five ornamental Malus cultivars. Subsequent examinations of pollen tube growth, fruit set and seed numbers showed that all were compatible with Cox, though some differences were found. Pollen of four of the five ornamental Malus cvs, viz. Golden Hornet, Hillieri, Winter Gold and Aldenhamensis, was as compatible with Cox as that of the dessert cultivars. No metaxenia effects on size, weight, colour, russet, acidity or sugar content were seen in the pollinated Cox fruit.     

论我国苹果品种的更新问题

‘国光’、‘金冠’、‘红星’、‘倭巾’、‘青香蕉’、‘大国光’等是我国主要栽培的苹果品种:其中‘国光’在辽宁省占比重60%—70%,在山东及黄河故道地区占30%—40%,虽然‘国光’在辽东半岛和胶东半岛适应性强、产量高、耐贮藏,但果个小,着色差在国际市场上缺乏竞争力,而且在黄河故道地区裂果严重,急需进行更新,‘金冠’、‘红星’贮藏性差、‘倭巾’、‘青香蕉’、‘大国光’品质不良,作者根据苹果发展趋势和我国具体条件提出一套更新品种组合.认为‘红富士’、‘华冠’(‘金冠’ב富士’)和‘华帅’(‘富士’ב新红星’)应是我国今后主要更新的品种,三者在生产中所占比重应在80%—90%,后两个品种,一个可代替‘金冠’,一个则代替‘红星’.     

Resolving the Parentage of the Apple Cultivar ‘Meran’

In the mid-1970s, a new apple variety named ‘Meran’ was discovered in South Tyrol (northern Italy), which harbours the largest continuous apple growing area in Europe. The cultivar was registered for varietal protection and patented in several countries, and was declared to be a cross of the varieties ‘Golden Delicious’ and ‘Morgenduft’ (synonym ‘Rome Beauty’). The parentage of ‘Meran’ has, however, been questioned, and the present study aimed to assess the descent of this cultivar by the combined use of molecular genetic and bioinformatic tools. Five accessions of ‘Meran’ were collected from three different European germplasm collections and analysed at 14 variable microsatellite DNA loci. Subsequently, computer software was used to allocate the most likely parent pair from a set of cultivars representative for the apple growing area of South Tyrol in 1975. The molecular genetic data clearly excluded ‘Morgenduft’ as a gene donor to ‘Meran’ and provided strong evidence that ‘Meran’ is a cross of the cultivars ‘Golden Delicious’ and ‘Jonathan’, confirming previous assumptions based on morphological traits of the tree and fruit.     

Have the Flowers,Fruitlets, Ripe Fruit and Leaves of Apples Cultivars Similar Compositions of Phenolic and Antioxidant Capacity?

Total and specific phenolic compounds and antioxidant capacity were quantified over two seasons in flowers, leaves, fruitlets, and ripe fruit at harvest of the apple cultivars ‘Brookfield’, ‘Granny Smith’ and ‘Fuji’, as well the crabapple cultivars ‘Hillary’ and ‘Manchurian’. The results showed that leaves had the highest phenol concentrations and antioxidant capacity. Phenolic and antioxidant content was progressively lower from flowers, fruitlets, and ripe harvested fruit. There were high concentrations of phloridzin in flowers and leaves, while concentrations were lower in fruitlets, peel, and entire fruit at harvest. The phenolic compound with the highest concentration in peel was quercetin glycoside, while in the entire fruit it was flavanols. The studied crabapple cultivars did not present differences between them in phenolic compounds and antioxidant capacity.