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牛结节性皮肤病是由牛结节性皮肤病病毒引起的一种急性、亚急性或慢性传染病。患病牛的临床症状主要为发热、消瘦、淋巴结肿大、皮肤水肿、局部形成结节或溃疡,影响肉牛增重、奶牛产奶量等,对牛养殖业可造成严重经济损失。疫苗防控动物疫病是一种高效、可控措施,对疫病的预防发挥重要作用。近年国内外研究人员对牛结节性皮肤病疫苗进行研究,取得一定成绩。本文对牛结节性皮肤病疫苗研究进行综述,为后续牛结节性皮肤病疫苗研发提供参考。 相似文献
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CH Annandale PC Irons VP Bagla UI Osuagwuh EH Venter 《Reproduction in domestic animals》2010,45(2):250-255
The objectives of this work were to determine the site of persistence of lumpy skin disease virus (LSDV) in bulls shedding the virus in semen for a period longer than 28 days, to determine if the virus is present in all fractions of semen and to study lesions that developed in the genital tract. Six serologically negative postpubertal bulls were experimentally infected with a virulent field isolate of LSDV. The polymerase chain reaction (PCR) was performed on sheath washes, vesicular fluid, supernatant and cell‐rich fractions of semen from day 10 to day 26 postinfection (p.i.). Bulls that were positive by PCR on the whole semen sample collected on day 28 p.i. were slaughtered and tissue samples from their genital tracts submitted for histopathological evaluation, immunoperoxidase staining, virus isolation and PCR. Two of the bulls developed severe lumpy skin disease (LSD) and were found to be shedding viral DNA in their semen on day 28 p.i. Viral DNA was identified in all semen fractions from all bulls, but mostly from the cell‐rich fraction and from the severely affected bulls. The PCR assay was positive on postmortem samples of testes and epididymides from the two severely affected bulls. Virus could be recovered from the testes of these two bulls and from the epididymis of one of them. Immunoperoxidase staining was positive for LSDV staining in sections of testes and epididymides exhibiting necrosis. This study suggests that the testis and epididymis are sites of persistence of LSDV in bulls shedding virus in semen for prolonged periods and revealed that viral DNA is present in all fractions of the ejaculate. 相似文献
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Goat pox virus was isolated during an outbreak of pox infection among goats in the Sersenk district, Iraq. The isolated virus grew on the chorioallantoic membranes of developing chick embryos and in primary lamb testis cell cultures. It was identified morphologically as a pox virus and serologically as a member of the Capripoxvirus group in the family Poxviridae. The isolated virus was designated the "Sersenk" strain. 相似文献
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H. H. Tantawi M. M. Awad M. O. Shony A. H. Alwan F. K. Hassan 《Tropical animal health and production》1980,12(1):30-34
The Sersenk strain of goat pox virus first isolated in Iraq, grew on chorioallantoic membranes (CAM) of developing chicken embryos producing generalised large pocks, 2 to 3 mm in diameter, on the third day post-inoculation. The virus killed the inoculated embryos. Replication of the virus in primary cultures of lamb testis cells induced a cytopathic effect (CPE) and plaque formation characteristic of pox viruses. It was antigenically related to reference strains of goat pox and sheep pox viruses. The virus was sensitive to ether and chloroform, failed to agglutinate erythrocytes and was strictly pathogenic to goats. 相似文献
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Goatpox (GTP), sheeppox (SPP) and lumpy skin disease (LSD) are three severe diseases of goat, sheep and cattle. Their typical clinical symptoms are characterized by vesicles, papules, nodules, pustules and scabs on animal skins. The GTP, SPP and LSD are caused by goatpox virus (GTPV), sheeppox virus (SPPV) and lumpy skin disease virus (LSDV), respectively, all of which belong to the genus Capripoxvirus in the family Poxviridae. Several capripoxvirus (CaPV) isolates have been virulently attenuated through serial passaging in vitro for production of live vaccines. CaPV-based vector systems have been broadly used to construct recombinant vaccines for delivering foreign antigens, many of which have been demonstrated to induce effective immune protections. Homologous recombination is the most commonly used method for constructing recombinant CaPVs. Here, we described a methodology for generation of recombinant CaPVs by the homologous recombination, and further reviewed CaPV-vectored vaccines for delivering foreign antigens. 相似文献
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Comparison of the external dimensions of capripoxvirus isolates 总被引:4,自引:0,他引:4
No significant difference was found between the external dimensions of the M forms of isolates of sheep pox virus from Nigeria, sheep and goat pox virus from Kenya and lumpy skin disease virus from South Africa. Earlier reports that isolates of capripoxvirus can be distinguished by their relative sizes could not be substantiated. 相似文献
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Gulbahar MY Cabalar M Gul Y Icen H 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2000,47(3):173-181
The present study describes the detection of sheeppox virus antigen in various lamb tissues, using an immunohistochemical technique, in sheeppox cases which occurred naturally. Sheeppox viral antigen was detected in the cytoplasm of sheeppox cells and degenerated epithelial cells of the skin, lungs and digestive tract involving typical sheeppox lesions. Nuclear staining was also observed in some typically deformed nuclei of sheeppox cells. The immunostaining of sheeppox virus showed a correlation with the presence of sheeppox cells and degenerated epithelial cells resembling them. Additionally, in order to confirm the presence of sheeppox virus in the skin and lung samples, direct electron microscopy was performed and sheeppox virus was only demonstrated in two skin samples. 相似文献
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The current status of sheep pox disease 总被引:12,自引:0,他引:12
Bhanuprakash V Indrani BK Hosamani M Singh RK 《Comparative immunology, microbiology and infectious diseases》2006,29(1):27-60
Sheep are the moving banks of shepherds and their economic contribution in terms of meat, wool and skin/hide is immense. Various infectious diseases jeopardize the optimum productivity; among which sheep pox is more important as the disease restricts the export of sheep and their products besides other economic losses. Although, clinical signs are indicative of the disease but a laboratory confirmation is necessary for unequivocal diagnosis and studying epidemiology. The causative agent, sheep pox virus (SPV), is antigenically and genetically closely related to goat pox virus (GPV) and lumpy skin disease virus (LSDV), the other members of the genus capripox virus. In some countries, SPV and GPV are cross infective to small ruminants posing problem in diagnosis and epidemiology. However, recent studies have showed that the viruses are phylogenetically distinct and can be differentiated by molecular tools. Prophylaxis using attenuated vaccines is the choice of control measure as the immunity is long lasting. Detailed information on isolation, identification, pathology, epidemiology, diagnosis and prophylaxis would not only help in updating the knowledge of scientific fraternity but will be useful to the policy makers in order to formulate appropriate measures for control and eradication of the disease. This synthesis is to present an up-to-date review of the disease and its control to provide the reader with an overview of the problem. 相似文献
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Afshar A Bundza A Myers DJ Dulac GC Thomas FC 《The Canadian veterinary journal. La revue veterinaire canadienne》1986,27(8):301-306
Under conditions of a maximum security laboratory, four cross-bred sheep were inoculated intradermally only or intradermally and intratracheally with a West African isolate of sheep pox virus. All sheep had increased temperature and depression by the fourth or fifth day after infection. Nasal and lacrimal discharge and coughing occurred in all sheep but were more severe in sheep receiving the virus via the tracheal route. From the fifth day after infection, numerous papular erythematous skin lesions developed at the inoculation sites. These were 3-7 mm in diameter and gradually became nodular. Some of these lesions healed and others coalesced to form tumorlike masses. In one sheep, euthanized 14 days after intradermal and intratracheal inoculation, nodular lesions were found in the skin around the eyes, nostrils, oral and perianal regions, the mucosa of the rumen and throughout the lungs. Histologically, skin nodules were characterized by ischemic necrosis, vasculitis, microvesicualtion, eosinophilic cytoplasmic inclusions in the dermal epithelial cells and vacuolar nuclear degeneration. The pulmonary lesion was that of proliferative alveolitis with occasional cytoplasmic inclusions in the alveolar cells and macrophages. Ultrastructurally, large cuboidal virus particles were found both in the skin lesion and inoculated tissue cultures. The sheep pox virus structure was easily distinguished from contagious ecthyma virus, a parapoxvirus which causes sporadic disease in Canada. Serum neutralizing antibodies developed in all the sheep by 14 days postinfection.
The clinical and pathological characteristics of experimental sheep pox produced with this West African isolate were similar to those caused by Neethling virus of lumpy skin disease in cattle.
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羊痘病毒G蛋白偶联趋化因子受体基因序列分析及其在鉴别病毒种属上的作用 总被引:1,自引:0,他引:1
为明确G蛋白偶联趋化因子受体(GpCR)基因在羊痘病毒种属鉴别中的作用,本实验对3株绵羊痘病毒(SPV)和2株山羊痘病毒(GPV)弱毒疫苗株的GpCR进行了克隆测序,并与GenBank中登录的21个羊痘病毒属成员相关序列进行了比对。核酸同源性分析表明SPV不同毒株之间同源性达到99.5%~99.8%;GPV之间同源性达到98.8%~99.8%;GenBank上公布的疙瘩皮肤病病毒(LSDV)之间同源性达到100%。而GPV、SPV和皮肤疙瘩病毒两两比对的同源性仅达到94.2%~95.9%。对GpCR的系统进化树分析可以看出GPV、SPV和LSDV分别位于不同的进化分枝上,而GPV与LSDV具有更近的亲缘关系。GpCR基因在鉴别GPV属和流行病学分析中具有重要价值。 相似文献
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为建立鉴别绵羊痘病毒(SPPV)和山羊痘病毒(GTPV)的检测方法,本研究针对这2种病毒的基因组序列,分别设计2对特异性引物,通过对引物浓度、退火温度等的优化,建立了快速鉴别检测SPPV和GTPV的双重PCR方法。该方法分别扩增出SPPV长度为177 bp和GTPV长度为222 bp的目的片段。特异性试验结果显示,该方法对牛疙瘩皮肤病病毒、犬细小病毒、大肠杆菌O157、沙门氏菌、健康羊组织和牛组织均无扩增。敏感性试验显示,该方法最低可检测1.725×107copies/μL的SPPV和1.71×106copies/μL的GTPV。应用该方法对50份临床病料样品进行检测的结果与病毒分离鉴定结果一致,均检出5份感染GTPV的病料和2份感染SPPV的病料,表明该方法可以用于临床病料样品的检测。 相似文献
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I E Anderson H W Reid P F Nettleton C J McInnes D M Haig 《Veterinary immunology and immunopathology》2001,83(3-4):161-176
In sheep infected with the parapoxvirus orf virus, primary infection orf skin lesions developed and resolved within 8 weeks. Reinfection lesions were smaller and resolved within 3 weeks. The host response in the skin was characterized by an accumulation of neutrophils, dendritic cells, CD4+ T cells, CD8+ T cells, B cells and T19+ gammadelta T cells. The magnitude of this accumulation paralleled orf virus replication in the skin. In situ hybridization was used to detect cells expressing interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha) and interleukin-4 (IL-4) mRNAs in orf skin. Cells expressing IL-4 mRNA were not detected at any time after infection. Cells expressing IFN-gamma mRNA were detected after reinfection but not after primary infection. Cells expressing TNF-alpha mRNA included epidermal cells, vascular endothelium and uncharacterized cells that increased more rapidly in the skin after reinfection compared to primary infection. The results are consistent with a prominent role for IFN-gamma in the host immune response controlling the severity of the disease. 相似文献