Expression Pattern of CD34 at the Maternal–Foetal Interface During Pregnancy in Pigs

The pig exhibits a non‐invasive, epitheliochorial placentation. Adhesion molecules are indispensable for successful implantation and establishment of placentation. CD34 is an adhesion molecule belonging to the immunoglobulin superfamily (IgSF). To take the first step to investigate the role of CD34 in placentation, we examined the expression pattern of CD34 at the maternal–foetal interface in Yorkshire gilts on days 15, 26, 50 or 95 and in Meishan gilts on days 26, 50 or 95 of pregnancy (n = 3 gilts/breed/day of pregnancy) by immunohistochemical technique. The CD34‐positive signals were detected in uterine luminal epithelium and trophectoderm in Yorkshire pigs; the staining for CD34 was located in trophectoderm but barely detectable at the uterine luminal epithelium on day 15 of pregnancy. Then, the expression of CD34 increased dramatically in both the uterine luminal epithelium and trophectoderm by day 26, and weak staining intensity was observed at the maternal–foetal interface on days 50 and 95 of pregnancy. The expression pattern of CD34 in Meishan pigs is similar to that in Yorkshire pigs except that only a few positive signals were observed at the luminal epithelium on day 26 of pregnancy. These results suggest that CD34 may be involved in mediating the cell‐to‐cell adhesion between trophectoderm and the luminal epithelial cells during early pregnancy in pigs.     

Characterization of porcine uterine estrogen sulfotransferase

A quantitative trait locus (QTL) for uterine capacity is located on chromosome 8. Comparison of porcine and human genetic maps suggested that the estrogen sulfotransferase (STE) gene may be located near this region. The objectives of this study were to clone the full coding region for STE, compare endometrial STE gene expression between Meishan and White composite pigs during early pregnancy, and map the STE gene. We obtained a clone (1886 bp) containing the full coding region of STE by iterative screening of an expressed sequence tag library. Endometrial STE mRNA expression in White composite gilts was determined by Northern blotting on days 10, 13, and 15 of the estrous cycle; and on days 10, 13, 15, 20, 30, and 40 of pregnancy. STE mRNA expression was elevated (P<0.01) on days 20 and 30 of pregnancy compared to other days of the cycle or pregnancy. Endometrial STE mRNA expression during early pregnancy, determined using real-time RT-PCR, was elevated (P<0.01) on day 20 compared to day 15, decreased (P=0.02) between days 20 and 30, and decreased further (P<0.01) between days 30 and 40 in both Meishan and White composite pigs. Expression of STE mRNA was greater (P=0.01) in White composite pigs compared to Meishan pigs. Using a microsatellite from an STE containing BAC genomic clone, the STE gene was mapped to 65 centimorgans on chromosome 8. Because STE mRNA expression differs between Meishan and White composite pigs, the STE gene may be a candidate for the uterine capacity QTL.     

Experimental inoculation of pregnant swine with type 1 bovine viral diarrhoea virus

The ability of bovine viral diarrhoea virus type 1 (BVDV-1) to induce transplacental infection in pigs was evaluated. Control pigs (n = 4) were sham-inoculated while infected pigs (n = 4) were intranasally inoculated with BVDV-1 on day 65 of gestation. Blood samples were tested throughout the study for BVDV and antibody to BVDV. On day 110 of gestation, a Caesarean section was performed. Serum was obtained for virus isolation and antibody determination from all piglets, and all experimental animals were killed. Tissues were collected for virus isolation and histopathology. Bovine viral diarrhoea virus was isolated on days 5 and 7 after infection and seroconversion was demonstrated in all infected gilts; however, BVDV was only isolated from one fetus from an infected pig. Viraemia and seroconversion were demonstrated in the pregnant gilts; however, transplacental infection at day 65 of gestation in the pig was not consistently demonstrated.     

Old,new and the newest concepts of inhibition of luteolysis during early pregnancy in pig

In 1977 Bazer and Thatcher proposed that maternal recognition of pregnancy in the pig involves the secretion of PGF(2alpha) towards the uterine lumen (exocrine) rather than towards the uterine venous drainage (endocrine) as occurs in the non-pregnant pig during the mid to late stages of the estrous cycle. The retrograde transfer of PGF(2alpha) from the venous blood and uterine lymph into the uterus and the ability of the uterine vein and artery wall to accumulate PGF(2alpha) could constitute a part of putative mechanism of corpus luteum protection during early pregnancy. A luteotropic/anti-luteolytic effect of PGE(2) in the pig also has been frequently demonstrated and it seems that the most effective agent in changing PGE(2):PGF(2alpha) secretion is estradiol. The role for oxytocin during luteolysis and early pregnancy is controversial. It appears, however, that the main function of this hormone is autocrine and/or paracrine stimulation of PGF(2alpha) secretion. Pig trophoblastic interferons, unlike those of ruminants, do not themselves exert an anti-luteolytic effect in pigs. It is likely, that cytokines and angiogenic growth factors are involved in the initiation of luteolysis and/or maintenance of corpora lutea (CL).A discovery of functional LH receptors in porcine endometrium opened a new possibility for this hormone in luteolysis and perhaps in recognition of pregnancy in pigs. The endogenous LH pulses can provoke prostaglandin secretion from endometrium in pigs. On the other hand prolongation of up-regulation of LH receptors in endometrium of early pregnant gilts can additionally increase angiogenic factor production before the process of implantation is completed. Finally new integrated concepts of luteolysis and inhibition of luteolysis in pigs based on selectively reviewed information are presented.     

A radioimmunoassay for porcine intrauterine folate binding protein.

A RIA was developed for porcine intrauterine folate binding protein (FBP). Displacement of [125I]FBP caused by increasing dilutions of uterine flushings collected from either d-15 pregnant or nonpregnant gilts or media from culture of endometrial tissue from d-15 pregnant or nonpregnant gilts was parallel to the displacement caused by the standard curve. Addition of known amounts of purified allantoic fluid FBP to dilutions of either intrauterine flushings or endometrial culture medium were measured accurately with the RIA. To test specificity, 2-mL samples of uterine flushings collected from d-15 pregnant and nonpregnant gilts were preincubated with 10 microCi of [3H]folic acid and then chromatographed using Sephadex G-100 (Sigma Chemical Co., St. Louis, MO). The fractions were subsequently assayed for radioactivity by liquid scintillation spectrophotometry and for FBP by RIA. The [3H]folic acid and FBP peaks coincided, indicating that the RIA is specific for FBP. Uterine flushings were collected on d 10, 11, 12, 13, 14, and 15 of the cycle or pregnancy from 1) White crossbred, 2) progesterone-treated White crossbred (200 mg of progesterone at 48 and 72 h after estrus), and 3) Meishan gilts and assayed for FBP. Total FBP increased 140-fold from d 10 to 15, and the pattern of change across day did not differ between pregnant and nonpregnant gilts. Progesterone treatment increased intrauterine FBP content on d 10 and 11. No difference in FBP concentrations was detected between White crossbred and Meishan gilts. These results indicate that the RIA for FBP is valid, allowing measurement of this protein in uterine flushings and endometrial culture medium. The onset of FBP secretion by the uterus between d 10 and 15 of the cycle or pregnancy is influenced by the timing of onset of progesterone influence in a manner similar to the endometrial proteins uteroferrin and retinol binding protein. In contrast to these endometrial proteins, FBP concentrations are similar in Meishan and White crossbred gilts.     

不同猪种ESR基因多态性的PCR-RFLP分析

运用PCR-RFLP方法,检测了杜洛克、约克夏、长白猪、皮特兰、梅山猪、二花脸猪、枫泾猪、荣昌猪、苏太猪等9个国内外猪种的ESR基因PvuⅡ位点多态性。结果表明,在约克夏猪、二花脸猪、枫泾猪和苏太猪中检测到了AA、AB、BB 3种基因型,在杜洛克猪、皮特兰猪中检测到AB、BB 2种基因型,在长白猪、梅山猪、荣昌猪中只检测到了AB基因型。在本研究中不同品种的A、B基因频率差异显著,总体上在国外猪群体中A等位基因为优势等位基因,而地方猪种中B等位基因为优势等位基因。     

Differential growth factor content of uterine luminal fluids from large white and prolific Meishan pigs during the estrous cycle and early pregnancy

Uterine luminal fluids (ULF) from Large White (LW) and prolific Chinese Meishan (MS) gilts were compared with respect to their peptide growth factor content during an estrous cycle and early pregnancy. Insulin-like growth factor-I (IGF-I) was quantitated by RIA; in vitro growth promoting properties of uterine luminal fluid mitogen (ULFM) were measured by [3H]thymidine incorporation into DNA of quiescent AKR-2B fibroblastic cells in culture. Peak concentrations (pg/microgram ULF protein) of IGF-I in ULF of Large White and Meishan gilts, respectively, were: estrous cycle, 9.8 +/- 1.4 (on d 10) and 39.7 +/- 7.8 (on d 12); gestation, 13.1 +/- 3.2 (on d 8 and 10) and 11.9 +/- 2.1 (on d 12), with differences among days (except d 10, P greater than .5) being affected by breed (P less than .10). For both breeds, there was a rapid decline in IGF-I concentrations by d 14 of the cycle and of pregnancy. Uterine luminal fluid mitogen activity was greater (P less than .01) for LW than for MS gilts on d 10 to 14 of an estrous cycle and gestation and diminished in a time-dependent manner in both breeds. No correlation was observed between IGF-I concentrations and uterine weights for either breed. In contrast, a negative correlation between uterine weight and ULFM activity was detected for cyclic (MS: r = -.855, P less than .10; LW: r = -.834, P less than .05) and pregnant (MS: r = -.806, P less than .10; LW: r = -.928, P less than .05) gilts.(ABSTRACT TRUNCATED AT 250 WORDS)     

Myometrial electrical activity during pregnancy and parturition in the pygmy goat

To quantify the pattern of myometrial activity during gestation and parturition, one bipolar electrode was implanted on each uterine horn of four bilaterally pregnant pygmy goats between days 102 and 120 of gestation. After a recovery period, electromyographic recordings were made for at least six hours per day and continuously when parturition was judged to be imminent. During late gestation myometrial activity occurred as discrete episodes of myometrial electrical activity (EMEA) with a mean duration ranging from 6.2 +/- 2.07 to 8.3 +/- 1.60 minutes. The mean interval between two successive EMEAs ranged from 45.8 +/- 19.95 to 74.7 +/- 42.27 minutes. In three of the four goats these characteristics were not significantly different from the two uterine horns. Parturition was preceded by a prolonged period (eight to 12 hours) of myometrial quiescence. It was only from 19 to 15 hours before expulsion of the first kid that total duration of EMG activity increased. This finally resulted in the regular occurrence of bursts which occupied 25 to 30 per cent of the recording time. The results demonstrate that, as in the sheep, cow and pig, the myometrium is active during late pregnancy. It is postulated that luteolysis coincides with the prolonged period of myometrial quiescence which precedes the onset of the parturient pattern of uterine activity.     

Cystine aminopeptidase activity (oxytocinase) in pregnant guinea pigs: normal and infected with Campylobacter fetus.

The cystine aminopeptidase (CAP) activity was determined in plasma from guinea pigs at various stages of gestation and in pregnant guinea pigs infected with Campylobacter fetus subspecies intestinalis. The CAP activity decreased with length of gestation, which is in contrast to the changes in activity in persons. Infected guinea pigs near the time of abortion can be distinguished from normal guinea pigs on the basis of plasma CAP activity, if the plasma samples are taken 1 to 2 days before abortion. Among pregnant guinea pigs at the same stage of gestation, CAP activity was lower for infected than for normal pregnant animals. This test might be used to indicate probable disruption of placentation and the onset of abortion in the guinea pig.     

Cloning of Porcine SKP1 Gene and it's Expression Studies between Meishan and Duroc Pigs with Follicle

To detect the expression pattern of S-phase kinase association protein 1 (SKP1) gene in during porcine follicle development.The coding sequence (CDS) of SKP1 gene was cloned from porcine follicular tissue.To analyze the tissue expression profiling of SKP1 gene and its expression level in follicles with different diameter (S,M1,M2,L) from Duroc and Meishan pigs were investigated by Real-time fluorescence quantitative PCR.The results showed that the length of CDS of porcine SKP1 was 492 bp,and it's similarity with that of Ovis aries,Homo sapiens,Pan troglodytes,Bos taurus and Rattus were 93.10%,92.90%,92.29%,91.89% and 89.86%,respectively.SKP1 mRNA was expressed in all tested tissues (muscle,fat,heart,liver,spleen,lung,kidney,stomach,duodenum,ovarian,tubal,uterine,uterine horn,pituitary,corpus luteum,brain,hypothalamus),and especially high in uterine,spleen and tubal.Both in Meishan pig.The expression of SKP1 mRNA in S,M1 and M2 follicles were higher than Duroc pig.Especially,the expression in Meishan pig M1 and M2 follicles were significantly higher than Duroc pig (P<0.01),respectively 2.39,2.82 times,and the expression of L follicle in Duroc pig was higher than Meishan pig,which suggested that SKP1 gene might be involved in the process of procine's follicular development.     

猪SKP1基因的克隆及其在梅山猪与杜洛克猪卵泡中的表达研究

为探索SKP1(S-phase kinase association protein 1)基因在猪卵泡中的表达规律,本试验从猪卵泡组织中克隆了猪SKP1基因CDS区全长序列,采用Real-time PCR方法检测SKP1基因在不同组织中的表达谱,进一步分析了该基因在梅山猪和杜洛克猪S卵泡、M1卵泡、M2卵泡、L卵泡中的表达。结果表明,经克隆测序,得到了猪SKP1基因492 bp编码区全长序列,与羊、人、黑猩猩、牛、大鼠的同源性分别为93.10%、92.90%、92.29%、91.89%、89.86%。SKP1基因在各组织中均有不同程度的表达(肌肉、脂肪、心脏、肝脏、脾脏、肺脏、肾脏、胃、十二指肠、卵巢、输卵管、子宫、子宫角、垂体、黄体、大脑、下丘脑),其中在子宫、脾脏、输卵管中表达量较高。SKP1基因的表达量在梅山猪S卵泡、M1卵泡和M2卵泡中的表达量均高于杜洛克猪,特别是在梅山猪M1卵泡和M2卵泡中SKP1基因的表达量极显著高于杜洛克猪(P<0.01),分别达到了2.39、2.82倍,而在L卵泡中的表达量却是杜洛克猪高于梅山猪,结果提示SKP1基因可能参与猪卵泡发育过程。     

Ultrasonographic studies on the reproductive tract of mares after parturition: effect of involution and uterine fluid on pregnancy rates in mares with normal and delayed first postpartum ovulatory cycles

During breeding of mares, ultrasonographic detection of uterine fluid accumulations in the first postpartum ovulatory period was associated with significantly decreased pregnancy rates, when compared with rates in control mares (P less than 0.005). The previously gravid uterine horn was recognized as the larger horn, when assessed for size by ultrasonography, for a mean of 21 days (range, 15 to 25 days) after parturition. On the basis of similar measurements obtained during 3 ultrasonographic scans (5-day period), uterine involution was determined to be completed in a mean of 23 days (range, 13 to 29 days). Progestin treatment did not affect uterine size, fluid accumulation, or rate of involution after parturition. However, delaying the first postpartum ovulation with 8 days of progestin treatment significantly improved pregnancy rates (P less than 0.05). More (P less than 0.05) mares became pregnant (23 of 28, 82%) when ovulation occurred after day 15 in the first postpartum ovulatory period, compared with those mares that ovulated before day 15 (6 of 12, 50%). We concluded that ultrasonographic detection of uterine fluid and postpartum progestin treatment can be used to manipulate breeding strategies and to improve pregnancy rates in mares bred during the first postpartum ovulatory period.     

Tumour Necrosis factor-α Receptors are Present in the Corpus Luteum Throughout the Oestrous Cycle and During the Early Gestation Period in Pigs

To determine the physiological significance of tumour necrosis factor‐α (TNFα) in the regulation of luteal functions in pig, this study was conducted to identify the presence of functional TNFα receptors in porcine corpora lutea (CL) throughout the oestrous cycle and the early gestation. The CL were isolated from pigs on days 4, 6, 8, 12 or 15 of the oestrous cycle (n=3; day 0 = oestrus) and days 15, 20 or 25 of gestation (n=3; day 0 = mating). A Scatchard analysis revealed the presence of a high‐affinity binding site for TNFα in all samples (dissociation constant; 2.7 ± 0.51 to 5.8 ± 0.50 nM ). The concentration of TNFα receptors was higher on day 15 of the oestrous cycle than on days 4 and 8 of the oestrous cycle (p < 0.05). Furthermore, TNFα receptor concentrations in the CL on days 15, 20 and 25 of gestation were significantly lower than on day 15 of the oestrous cycle (p < 0.05). On day 9 of the oestrous cycle, exposure of cultured luteal cells to 0.06–60 nM TNFα stimulated prostaglandin (PG) F_2α and PGE₂ secretion in a dose‐dependent manner (p < 0.05). These results indicate that functional TNFα receptors are present in the porcine CL throughout the oestrous cycle and early gestation, and suggest that TNFα plays one or more physiological roles in regulating CL function throughout the oestrous cycle and the early gestation period. In addition, TNFα receptor concentration in the CL of the late luteal stage (day 15) of the oestrous cycle was higher than on the respective day in the early pregnant pig, suggesting that TNFα plays a role in accomplishing luteolysis in the porcine CL.     

太湖流域地方猪种遗传结构评估和体重体尺性状差异相关选择位点鉴定

旨在解析太湖流域地方猪品种内部遗传结构,鉴定梅山猪亚群间、二花脸群体间和米猪群体间体重体尺性状差异的候选基因.试验采集440头猪样本(代表太湖流域地方猪最全面血统)进行基因芯片分型,并使用该基因分型数据集进行多种群体遗传学分析,明确太湖流域地方猪品种内部的遗传结构,并鉴定梅山猪亚群间、以及二花脸和米猪群体间体重体尺性状...     

Structure and Steroidogenesis of the Placenta in the Antarctic Minke Whale (Balaenoptera bonaerensis)

There are few reports describing the structure and function of the whale placenta with the advance of pregnancy. In this study, therefore, the placenta and nonpregnant uterus of the Antarctic minke whale were observed morphologically and immunohistochemically. Placentas and nonpregnant uteri were collected from the 15th, 16th and 18th Japanese Whale Research Programme with Special Permit in the Antarctic (JARPA) and 1st JARPA II organized by the Institute of Cetacean Research in Tokyo, Japan. In the macro- and microscopic observations, the placenta of the Antarctic minke whale was a diffuse and epitheliochorial placenta. The chorion was interdigitated to the endometrium by primary, secondary and tertiary villi, which contained no specialized trophoblast cells such as binucleate cells, and the interdigitation became complicated with the progress of gestation. Furthermore, fetal and maternal blood vessels indented deeply into the trophoblast cells and endometrial epithelium respectively with fetal growth. The minke whale placenta showed a fold-like shape as opposed to a finger-like shape. In both nonpregnant and pregnant uteri, many uterine glands were distributed. The uterine glands in the superficial layer of the pregnant endometrium had a wide lumen and large epithelial cells as compared with those in the deep layer. On the other hand, in the nonpregnant endometrium, the uterine glands had a narrower lumen and smaller epithelial cells than in the pregnant endometrium. In immunohistochemical detection, immunoreactivity for P450scc was detected in most trophoblast cells, but not in nonpregnant uteri, suggesting that trophoblast epithelial cells synthesized and secreted the sex steroid hormones and/or their precursors to maintain the pregnancy in the Antarctic minke whale.     

母猪妊娠期长短影响因素分析及其对产仔数的影响

我国是养猪大国,但生产效率相较于发达国家仍然相差很大,缩短母猪的繁殖周期是提高猪场综合生产效率和经济效益的有效手段.本文通过分析四川某种猪场包括大约克和长白猪的15665条繁殖数据,从母猪品种、公猪品种、配种季节和胎次等不同因素分析其对母猪妊娠期长短的影响,以及妊娠期长短对母猪产仔数的影响.结果表明,品种对母猪妊娠期有...     

Infection of pigs by aerosols of Aujeszky's disease virus and their shedding of the virus

On three consecutive days, six pigs were exposed for 15 minutes to aerosols of Aujeszky's disease virus. The total estimated dose was 4·5 log₁₀ 50. Within each isolation room, a sentinel pig was placed on a deck two feet away from the infected pig. The breath of the pigs that had inhaled the aerosols was collected on days 3, 7 and 13. The respiratory and other clinical signs of the infected pigs resembled those in field cases of Aujeszky's disease. All the pigs infected with Aujeszky's disease virus seroconverted within seven to 10 days after infection. Among the sentinel pigs, clinical signs were minimal and only three seroconverted.     

Monitoring pregnancy in babirusa (Babyrousa babyrussa) with transabdominal ultrasonography.

This case report documents the application of transabdominal ultrasonography for detecting pregnancy and monitoring fetal development in an unanesthetized, unrestrained babirusa (Babyrousa babyrussa). A 4-yr-old multiparous female babirusa was trained to accept monitoring via transabdominal ultrasonography on a weekly basis postcoitus. Uterine changes associated with pregnancy were first detected at 30 days gestation. Three fetuses were first visualized on day 38, and two of the three fetuses were detectable until term. One fetus failed to develop and appeared to be resorbed between days 80 and 94 of the 161-day pregnancy. The growth of the remaining two fetuses was monitored via cranial measurements taken during the weekly scans throughout the pregnancy. By monitoring a subsequent pregnancy via ultrasound in the same female it was possible to confirm her tendency towards partial resorption, with earliest detection of uterine changes on day 32 and apparent resorption of one of three fetuses at approximately the midpoint of gestation. Two rectal scans of a different female under anesthesia at 28 and 50 days gestation showed stages of development comparable to those visualized transabdominally. However, the transabdominal scans produced better images of the reproductive tract, perhaps because of the close proximity of the pregnant uterus to the abdominal wall.     

A comparison of six methods used for detecting pregnancy in sheep

The speed and accuracy of 4 ultrasonic devices (Scanopreg, Pregmatic 3, Medata external probe and Medata rectal probe) were compared with udder scoring and harnessed vasectomised rams as methods of pregnancy diagnosis in 177 ewes, 59 of which were non-pregnant. When using the Scanopreg in ewes of greater than 50 days gestation, accuracy in pregnant ewes averaged 98% and in non-pregnant ewes 87%. The Pregmatic 3 was 97% accurate in pregnant ewes after 50 days gestation and was 96% accurate in non-pregnant ewes. Ewes were examined at between 85 and 185 per hour using these devices. The Medata external probe was 99% accurate in pregnant ewes of greater than 110 days gestation but less accurate in early gestation. This instrument averaged 89% accuracy in non-pregnant ewes and an examination rate of 29 to 35 ewes per hour was achieved. The Medata rectal probe averaged 85% accuracy in pregnant ewes after 70 days gestation. Accuracy averaged 94% in non-pregnant ewes. Ewes were examined at between 35 and 59 per hour using this device. After 130 days gestation 84% of ewes had firm, obviously enlarge udders and another 14% had slight to moderate udder development. After 130 days gestation thick, clear udder secretions were expressed from 18% of ewes and thick, milky udder secretions were expressed from 8%. Harnessed vasectomised rams raddled one of 118 pregnant ewes (an accuracy in pregnant sheep of 99%) and raddled 53% of non pregnant ewes. It was concluded that the ultrasonic pregnancy testing devices have a commercial role, especially as aids to decision making.(ABSTRACT TRUNCATED AT 250 WORDS)     

香猪基因组外显子SNPs检测及繁殖和体型性状候选基因分析

为从基因组水平上探究香猪性成熟早、产仔数少、体型小的分子机理,本研究下载与香猪在繁殖和体型性状方面有明显差异的梅山猪、杜洛克猪的重测序数据作为参照,对26头香猪、24头梅山猪和24头杜洛克猪的重测序数据进行SNPs检测和等位基因频率差值分析,筛选香猪基因组外显子上与梅山猪、杜洛克猪高度分化的SNPs位点,并使用AS-PCR方法和Sanger测序方法统计其中8个SNPs位点的群体等位基因频率。最后,对含有高度分化SNPs位点的基因进行GO功能富集和KEGG通路分析。结果显示,SNPs检测获得香猪基因组外显子上SNPs共236 710个,包括193个终止密码子丢失、1 238个终止密码子获得、90 945个错义SNPs和144 334个同义SNPs。得到1 046个香猪高度分化SNPs,包括429个错义SNPs、2个终止子丢失、6个终止子获得和609个同义SNPs,影响524个蛋白质编码基因;并发现X染色体上44-58 Mb的一段富含高度分化SNPs的热点区域。群体等位基因频率验证结果与重测序结果一致,鉴定了8个位点均为香猪与梅山猪、杜洛克猪高度分化的SNPs。对524个含有高度分化SNPs的基因进行GO和KEGG分析,富集到卵母细胞减数分裂、雌激素信号途径等繁殖相关通路;GO功能注释到细胞内雌激素受体信号、纤毛运动等繁殖相关条目,骨骼系统形态发生、骨骼发育、成骨细胞分化等体型相关条目。得到含有香猪高度分化SNPs的18个繁殖性状候选基因PTGFR、TRO、DNAH17、PKDREJ、KAT8、DNAI2、VDR、TEX14、QSOX1、AR、WNK3、ADCY3、SPEF1、MIGA2、SLC2A8、PSMF1、TBC1D20、IFT172和7个体型相关基因IBSP、NR6A1、HSPG2、TARS、PDZD2、FGF4、AMER1,可能是决定香猪性成熟早、产仔数少、体型小的关键基因。