Crystal Structure of the Solid Electrolyte, RbAg4I5

The crystal structure of the solid electrolyte RbAg(4)I(5) has been determined from single-crystal x-ray diffraction counter data. There are four RbAg(4)I(5) in a cubic unit cell with a = 11.24 A. The structure refinement, by least squares, is based on space group P4(1)3 (O(7)). The arrangement of the iodide ions is similar to that of the manganese atoms in beta-manganese, and provides 56 iodide tetrahedra per unit cell, which share faces in such manner as to provide diffusion paths for the silver ions. The occurrence of the two low-temperature phases of RbAg(4)I(5) has been established by x-ray diffraction and optical examination.     

Protein, DNA, and virus crystallography with a focused imaging proportional counter

A set of programs has been developed for rapid collection of x-ray intensity data from protein and virus crystals with a commercially available two-dimensional focused geometry electronic detector. The detector is compact and portable, with unusually high spatial resolution comparable to that used in oscillation photography. It has allowed x-ray data collection on weakly diffracting crystals with large unit cells, as well as more conventional "diffractometer-quality" crystals. The quality of the data is compared with that from oscillation photography and automated diffractometry in the range of unit cells from 96.3 to 383.2 angstroms. Isomorphous and anomalous difference Pattersons, based on detector data, are shown for a variable surface glycoprotein mercury derivative and for a repressor-DNA bromine derivative, which has been solved at 7 angstroms with detector data only.     

基于人工神经网络的道路表面降雨水深预测

建立了基于人工神经网络的道路表面水膜厚度预测模型，通过试验数据的训练确定权重和阈值，经过检验样本的检验，能够很好地预测道路表面的水膜厚度。结果表明，本文建立的人工神经网络模型用于道路表面水膜厚度预估可行。     

Renin and angiotensin: the complete system within the neuroblastoma x glioma cell

Cells of the homogeneous hybrid line neuroblastoma x glioma (NG108-15) have many neuronal properties. Immunocytochemical tests show that they contain both immunoreactive renin and angiotensin; direct radioimmunoassays show that they are positive for renin, angiotensin I, and angiotensin II; enzymatic assays show that they contain angiotensinogen and converting enzyme as well. The renin appears to be present in an enzymatically inactive form that can be activated by trypsin and then blocked by antiserum to purified mouse submaxillary renin. Renin concentration and activity are increased by enhancing cellular differentiation with dibutyryl cyclic adenosine monophosphate or by serum withdrawal. These findings demonstrate a complete renin-angiotensin system within these neuron-like cells, and suggest that activation of intracellular renin could generate angiotensin II.     

黔中马尾松近熟林空间结构特征及其调控

林分空间结构是描述林分结构极其变化的重要指标,是森林经营与管理成功的标志。利用随机抽样方法在黔中马尾松Pinus massoniana可持续经营管理试点（安顺市平坝区）设置100 m×100 m马尾松近熟林固定样地,采用10 m×10 m网格调查每株林木的树高、胸径和坐标数据,主要以混交度、大小比数和角尺度的一元和二元分布研究马尾松林的空间结构特征。结果表明:①该林分中马尾松在株数和断面积比例上占明显优势,种内竞争对马尾松生长影响很大,整个林分以零度混交为主,空间异质性不高;②在整个林分中,大部分林木处于随机分布,胸径差异不显著,伴生树种主要空间分布格局为集聚分布;③模拟采伐有利于林分空间结构的调控,改善林分空间分布格局,为森林群落的正向演替以及优势种群的健康生长提供了有利空间条件。     

Structure of the hydrated alpha-Al(2)O(3) (0001) surface

The physical and chemical properties of the hydrated alpha-Al(2)O(3) (0001) surface are important for understanding the reactivity of natural and synthetic aluminum-containing oxides. The structure of this surface was determined in the presence of water vapor at 300 kelvin by crystal truncation rod diffraction at a third-generation synchrotron x-ray source. The fully hydrated surface is oxygen terminated, with a 53% contracted double Al layer directly below. The structure is an intermediate between alpha-Al(2)O(3) and gamma-Al(OH)(3), a fully hydroxylated form of alumina. A semiordered oxygen layer about 2.3 angstroms above the terminal oxygen layer is interpreted as adsorbed water. The clean alpha-Al(2)O(3) (0001) surface, in contrast, is Al terminated and significantly relaxed relative to the bulk structure. These differences explain the different reactivities of the clean and hydroxylated surfaces.     

Three-dimensional structure of an antigen-antibody complex at 2.8 A resolution

The 2.8 A resolution three-dimensional structure of a complex between an antigen (lysozyme) and the Fab fragment from a monoclonal antibody against lysozyme has been determined and refined by x-ray crystallographic techniques. No conformational changes can be observed in the tertiary structure of lysozyme compared with that determined in native crystalline forms. The quaternary structure of Fab is that of an extended conformation. The antibody combining site is a rather flat surface with protuberances and depressions formed by its amino acid side chains. The antigen-antibody interface is tightly packed, with 16 lysozyme and 17 antibody residues making close contacts. The antigen contacting residues belong to two stretches of the lysozyme polypeptide chain: residues 18 to 27 and 116 to 129. All the complementarity-determining regions and two residues outside hypervariable positions of the antibody make contact with the antigen. Most of these contacts (10 residues out of 17) are made by the heavy chain, and in particular by its third complementarity-determining region. Antigen variability and antibody specificity and affinity are discussed on the basis of the determined structure.     

Signaling for growth orientation and cell differentiation by surface topography in uromyces

The dimensions of the topographical signals for growth orientation and infection structure formation, a cell differentiation event that includes nuclear division, were determined for the stomatal penetrating rust fungus Uromyces appendiculatus. The differentiation signal was found to be a simple ridge on the substrate surface that had a markedly optimum height of 0.5 micrometer. Such ridges were microfabricated on silicon wafers by using electron-beam lithography. A similar ridge, in the form of a stomatal lip, was found associated with the stomatal guard cells of the bean (Phaseolus vulgaris) leaf. Ridge elevations greater than 1.0 micrometer or less than 0.25 micrometer did not serve as effective signals. Germ tubes of the fungus were highly oriented by ridge spacings of 0.5 to 6.7 micrometers. The data indicate that the fungus is able to distinguish uniquely minute differences in leaf surface topography in order to infect the host plant.     

Mercury: results on mass, radius, ionosphere, and atmosphere from mariner 10 dual-frequency radio signals

Analysis of the radio-tracking data from Mariner 10 yields 6,023,600 +/- 600 for the ratio of the mass of the sun to that of Mercury, in very good agreement with values determined earlier from radar data alone. Occultation measurements yielded values for the radius of Mercury of 2440 +/- 2 and 2438 +/- 2 kilometers at laditudes of 2 degrees N and 68 degrees N, respectively, again in close agreement with the average equatorial radius of 2439 +/- 1 kilometers determined from radar data. The mean density of 5.44 grams per cubic centimeter deduced for Mercury from Mariner 10 data thus virtually coincides with the prior determination. No evidence of either an ionosphere or an atmosphere was found, with the data yielding upper bounds on the electron density of about 1500 and 4000 electrons per cubic centimeter on the dayside and nightside, respectively, and an inferred upper bound on the surface pressure of 10(-8) millibar.     

基于LS-SVM的采摘机器人运动变结构控制

为了提高采摘机器人控制的精度，提出了一种基于支持向量机的变结构控制方法。利用支持向量机在线调整变结构控制律中的控制参数，克服了常规变结构控制方法中需预先设定趋近律参数的限制，既保留了传统趋近律的优点，又有效的改善了系统的控制品质，消除了系统抖振，使系统最终以理想方式在滑模面上运动，理论分析和仿真结果表明了所提出方法的有效性。     

A genetic polymorphism in the renin gene of Dahl rats cosegregates with blood pressure

Blood pressure is influenced by multiple genetic loci whose identities are largely unknown. A restriction fragment length polymorphism (RFLP) in the renin gene was found between Dahl salt-hypertension-sensitive (S) and Dahl salt-hypertension-resistant (R) rats. In an F2 population derived from crossing S and R rats, the renin RFLP cosegregated with blood pressure. One dose of the S-rat renin allele was associated with an increment in blood pressure of approximately 10 mmHg, and two doses of this allele increased blood pressure approximately 20 mmHg. From this it can be definitively concluded that in the rat the renin gene is, or is closely linked to, one of the genes regulating blood pressure.     

山地丘陵区社会经济数据空间化模型构建及应用

社会经济数据的空间化,是地学研究中解决数据结构不一致、空间单元不匹配和数据在空间单元内均一化问题的有效方法.本文基于多因素回归分析建立了社会经济数据空间化处理方法,选取重庆市丰都县为研究区,以人口密度和GDP规模为对象进行了回归建模,为社会经济数据的空间精细化研究提供借鉴.结果表明,采取向后剔除法准则的多元线性回归分析数据融合方法,较好地模拟了研究区社会经济数据的空间分布格局,拟合方程修正后的可决系数均达到0.80以上;将以乡镇为单元拟合的县域社会经济数据融合模型,应用于村域尺度社会经济数据的空间精细化表达,经验证数据吻合度普遍在80%以上,能够较好地实现村域尺度社会经济数据的空间化.因此,就研究区而言,基于多因素回归分析的社会经济数据空间化模型构建,较好地实现了对社会经济数据的反演,为创建区域范围内村域尺度连续的社会经济数据表面提供了支撑.     

A surface-tailored, purely electronic, mott metal-to-insulator transition

Mott transitions, which are metal-insulator transitions (MITs) driven by electron-electron interactions, are usually accompanied in bulk by structural phase transitions. In the layered perovskite Ca(1.9)Sr(0.1)RuO4, such a first-order Mott MIT occurs in the bulk at a temperature of 154 kelvin on cooling. In contrast, at the surface, an unusual inherent Mott MIT is observed at 130 kelvin, also on cooling but without a simultaneous lattice distortion. The broken translational symmetry at the surface causes a compressional stress that results in a 150% increase in the buckling of the Ca/Sr-O surface plane as compared to the bulk. The Ca/Sr ions are pulled toward the bulk, which stabilizes a phase more amenable to a Mott insulator ground state than does the bulk structure and also energetically prohibits the structural transition that accompanies the bulk MIT.     

An ABAGE-Like Metabolite of Botrytis cinerea Isolate BC4 Inhibited the Growth of Hypocotyls and Roots of Amaranthus retroflexus Seedlings

A metabolite, which had an inhibitory effect on plant growth, was isolated from cultural filtrate of Botrytis cinerea isolate BC4 by column chromatography on silica gel and preparative HPLC. Its structure was determined from HPLC-ESI MS, GC, IR, ^1H NMR and ^13C NMR spectral data, as well as chemical hydrolysis. The results showed that this metabolite was quite similar to abscisic acid-β-D-glucopyranosyl ester（ABAGE） in structure. The inhibitory effect of the ABAGE-like metabolite on plant growth was investigated using a weed Amaranthus retroflexus L. as a bioassay material. The results showed that it inhibited hypocotyls and roots growth of A. retroflexus （Amaranthus retroflexus L.） seedlings when its concentration was over 0.5 μM. The concentrations for 50% inhibition of hypocotyls and roots growth of A. retroflexus seedlings were 2.8 and 1.4 μM, respectively.     

Mass and local topography measurements of Itokawa by Hayabusa

The ranging instrument aboard the Hayabusa spacecraft measured the surface topography of asteroid 25143 Itokawa and its mass. A typical rough area is similar in roughness to debris located on the interior wall of a large crater on asteroid 433 Eros, which suggests a surface structure on Itokawa similar to crater ejecta on Eros. The mass of Itokawa was estimated as (3.58 +/- 0.18) x 10(10) kilograms, implying a bulk density of (1.95 +/- 0.14) grams per cubic centimeter for a volume of (1.84 +/- 0.09) x 10(7) cubic meters and a bulk porosity of approximately 40%, which is similar to that of angular sands, when assuming an LL (low iron chondritic) meteorite composition. Combined with surface observations, these data indicate that Itokawa is the first subkilometer-sized small asteroid showing a rubble-pile body rather than a solid monolithic asteroid.     

Solution structure of kistrin, a potent platelet aggregation inhibitor and GP IIb-IIIa antagonist.

The structure of kistrin, which is a member of a homologous family of glycoprotein IIb-IIIa (GP IIb-IIIa) antagonists and potent protein inhibitors of platelet aggregation, has been determined by two-dimensional nuclear magnetic resonance (NMR) spectroscopy. The 68-residue protein consists of a series of tightly packed loops held together by six disulfide bonds and has almost no regular secondary structure. Kistrin has an Arg-Gly-Asp (RGD) adhesion site recognition sequence important for binding to GP IIb-IIIa that is located at the apex of a long loop across the surface of the protein.     

Structure,mechanism, and regulation of the Neurospora plasma membrane H+-ATPase

Proton pumps in the plasma membrane of plants and yeasts maintain the intracellular pH and membrane potential. To gain insight into the molecular mechanisms of proton pumping, we built an atomic homology model of the proton pump based on the 2.6 angstrom x-ray structure of the related Ca2+ pump from rabbit sarcoplasmic reticulum. The model, when fitted to an 8 angstrom map of the Neurospora proton pump determined by electron microscopy, reveals the likely path of the proton through the membrane and shows that the nucleotide-binding domain rotates by approximately 70 degrees to deliver adenosine triphosphate (ATP) to the phosphorylation site. A synthetic peptide corresponding to the carboxyl-terminal regulatory domain stimulates ATPase activity, suggesting a mechanism for proton transport regulation.     

How do temporal and spectral features matter in crop classifi cation in Heilongjiang Province,China?

How to fully use spectral and temporal information for efficient identification of crops becomes a crucial issue since each crop has its specific seasonal dynamics. A thorough understanding on the relative usefulness of spectral and temporal features is thus essential for better organization of crop classification information. This study,taking Heilongjiang Province as the study area,aims to use time-series moderate resolution imaging spectroradiometer(MODIS) surface reflectance product(MOD09A1) data to evaluate the importance of spectral and temporal features for crop classification. In doing so,a feature selection strategy based on separability index(SI) was first used to rank the most important spectro-temporal features for crop classification. Ten feature scenarios with different spectral and temporal variable combinations were then devised,which were used for crop classification using the support vector machine and their accuracies were finally assessed with the same crop samples. The results show that the normalized difference tillage index(NDTI),land surface water index(LSWI) and enhanced vegetation index(EVI) are the most informative spectral features and late August to early September is the most informative temporal window for identifying crops in Heilongjiang for the observed year 2011. Spectral diversity and time variety are both vital for crop classification,and their combined use can improve the accuracy by about 30% in comparison with single image. The feature selection technique based on SI analysis is superior for achieving high crop classification accuracy(producers' accuracy of 94.03% and users' accuracy of 93.77%) with a small number of features. Increasing temporal resolution is not necessarily important for improving the classification accuracies for crops,and a relatively high classification accuracy can be achieved as long as the images associated with key phenological phrases are retained.     

Localization and physical property experiments conducted by Opportunity at Meridiani Planum

The location of the Opportunity landing site was determined to better than 10-m absolute accuracy from analyses of radio tracking data. We determined Rover locations during traverses with an error as small as several centimeters using engineering telemetry and overlapping images. Topographic profiles generated from rover data show that the plains are very smooth from meter- to centimeter-length scales, consistent with analyses of orbital observations. Solar cell output decreased because of the deposition of airborne dust on the panels. The lack of dust-covered surfaces on Meridiani Planum indicates that high velocity winds must remove this material on a continuing basis. The low mechanical strength of the evaporitic rocks as determined from grinding experiments, and the abundance of coarse-grained surface particles argue for differential erosion of Meridiani Planum.     

Relation between earthquakes, weather, and soil tilt

Two years of local earthquake, temperature, and rainfall data taken near a tiltmeter site were used in a study of the numerical relation between these phenomena and the recorded tilt response. A least-squares shaping and predictive error filter approach was used. The relations were ranked in part according to the root mean square (r.m.s.) error of fit across the entire sample space. The tilt data with an annual range of tilt of approximately 10 microradians were fitted to the combined weather data of temperature and rainfall with a 0.75-microradian r.m.s. error. The best fit of earthquakes to these same tilt data is the subclass of events with magnitude (M) > 2.5 within 30 kilometers of the tilt site. The filter that mapped earthquakes to tilt yielded a 1.03-microradian r.m.s. error. The most unusual tilt anomaly over the entire 2-year period has the best fit of rainfall to the data for any single month of the entire data set. This unusual anomaly was the basis of an erroneously predicted earthquake (M approximately 5). These data indicate that if there are premonitory earthquake signals, they are buried in local meteorlogical noise. Separating an earthquake anomaly from the response to surface phenomena becomes more difficult as the earthquake anomaly lead time approaches the rise time of the soil to weather and seasonal variations.