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1.
Recombinant inbred lines (RILs) derived from a cross between Brassica rapa L. cv. ‘Sampad’, and an inbred line 3‐0026.027 was used to map the loci controlling silique length and petal colour. The RILs were evaluated under four environments. Variation for silique length in the RILs ranged from normal, such as ‘Sampad’, to short silique, such as 3‐0026.027. Three QTL, SLA3, SLA5 and SLA7, were detected on the linkage groups A3, A5 and A7, respectively. These QTL explained 36.0 to 42.3% total phenotypic variance in the individual environments and collectively 32.5% phenotypic variance. No additive × additive epistatic interaction was detected between the three QTL. Moreover, no QTL × environment interaction was detected in any of the four environments. The number of loci for silique length detected based on QTL mapping agrees well with the results from segregation analysis of the RILs. In case of petal colour, a single locus governing this trait was detected on the linkage group A2. 相似文献
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3.
Inheritance of petal colour and its independent segregation from seed colour in Brassica rapa 总被引:1,自引:0,他引:1
The inheritance of petal (flower) colour and seed colour in Brassica rapa was investigated using two creamy‐white flowered, yellow‐seeded yellow sarson (an ecotype from Indian subcontinent) lines, two yellow‐flowered, partially yellow‐seeded Canadian cultivars and one yellow‐flowered, brown‐seeded rapid cycling accession, and their F1, F2, F3 and backcross populations. A joint segregation of these two characters was examined in the F2 population. Petal colour was found to be under monogenic control, where the yellow petal colour gene is dominant over the creamy‐white petal colour gene. The seed colour was found to be under digenic control and the yellow seed colour (due to a transparent coat) genes of yellow sarson are recessive to the brown/partially yellow seed colour genes of the Canadian B. rapa cvs.‘Candle’ and ‘Tobin’. The genes governing the petal colour and seed colour are inherited independently. A distorted segregation for petal colour was found in the backcross populations of yellow sarson × F1 crosses, but not in the reciprocal backcrosses, i.e. F1× yellow sarson. The possible reason is discussed in the light of genetic diversity of the parental genotypes. 相似文献
4.
Shuancang Yu Fenglan Zhang Xiuyun Zhao Yangjun Yu Deshuang Zhang 《Plant Breeding》2011,130(5):580-583
5.
Quantitative trait locus analysis of seed germination,seedling vigour and seedling‐regulated hormones in Brassica napus
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Tung C. T. Nguyen Suzanne R. Abrams Wolfgang Friedt Rod J. Snowdon 《Plant Breeding》2018,137(3):388-401
Good germination and seedling vigour are major breeding targets in winter oilseed rape (Brassica napus), because seedling vigour and prewinter crop establishment are closely associated with postwinter growth and yield. Here, we identified quantitative trait loci (QTL) related to germination, seedling vigour and seedling‐regulated hormones in a doubled haploid (DH) mapping population from a cross between winter oilseed rape parents with high vigour (Express 617) and low vigour (1012‐98). By phenotyping in a climate‐controlled glasshouse, we identified a total of 13 QTL on nine chromosomes for germination and seedling‐related traits at 7 and 14 days after sowing (DAS), explaining up to 11.2% of the phenotypic variation for seedling vigour. Forty‐seven metabolic QTL on 15 chromosomes were identified for auxin, abscisic acid (ABA) and dihydrophaseic acid (DPA) at 5 and 12 DAS, explaining up to 49.4% of phenotypic variation in seedling hormone composition. Multitrait QTL hot spots contribute to our understanding of the genetics and metabolomics of germination and seeding vigour in B. napus, and represent potential targets to breed high‐vigour cultivars. 相似文献
6.
Detection of loci controlling seed glucosinolate content and their association with Sclerotinia resistance in Brassica napus 总被引:1,自引:0,他引:1
A genetic linkage map of Brassica napus constructed from a cross between a low glucosinolate cultivar ‘H5200’ and a high glucosinolate line ‘NingRS‐1’ was used to identify loci associated with seed glucosinolate content and to understand the association between specific glucosinolate components and Sclerotinia resistance. Seed glucosinolate content was assessed by standard High pressure Liquid Chromatogram (HPLC) protocol. Seven components of seed glucosinolate, including four types of aliphatic glucosinolate, two types of indolyl glucosinolates and one aromatic glucosinolate were detected in the seeds. Three quantitative trait loci (QTLs) were identified for seed total glucosinolate content. From three to 15 loci were found to be responsible for different types of glucosinolates, and by comparing the overlapped intervals, eight genomic regions were defined. One of the nine loci associated with aliphatic glucosinolate content was found to be associated with Sclerotinia resistance on the leaf at the seedling stage, and one locus, responsible for 3‐indolyl‐methyl glucosinolate content, was probably linked with Sclerotinia resistance on the stem of the maturing plant. The association between seed glucosinolate content and Sclerotinia resistance is discussed. 相似文献
7.
The objective of this study was to determine quantitative trait loci (QTL) underlying ten floral and related traits in Aquilegia. The traits assessed were calyx diameter, corolla diameter, petal length, petal blade length, sepal length, sepal width, spur length, spur width, plant height and flower number. These are important traits for ornamental value and reproductive isolation of Aquilegia. QTL analysis of these traits was conducted using single‐marker analysis and composite interval mapping (CIM). We used an F2 population consisting of 148 individuals derived from a cross between the Chinese wild species Aquilegia oxysepala and the cultivar Aquilegia flabellata ‘pumila’. Resulting CIM analysis identified 39 QTLs associated with these traits, which were mapped on seven linkage groups. These QTLs could explain 1.22–53.28% of the phenotypic variance. Thirty‐one QTLs, which explained more than 10% of the phenotypic variation, were classified as major QTLs. Graphical representations of the QTLs on seven linkage groups were made. Our research provides the potential for future molecular assisted selection breeding programmes and the cloning of target genes through fine mapping. 相似文献
8.
Yanan Liu Hongwei Zhang Xuhui Li Feng Wang Demar Lyle Lianjun Sun Guoying Wang Jianhua Wang Li Li Riliang Gu 《Plant Breeding》2019,138(1):29-37
Quantitative trait locus (QTL) mapping for seed longevity is essential for breeding modern cultivars with resistance to deterioration during postharvest storage. The inbred lines X178 and I178 showed large differences in seed vigour after artificial aging treatment, while they had similar performances in terms of most agronomic traits. An F2:3 population and a recombinant inbred line (RIL) population were generated to map QTL after 5 days under artificial aging conditions. Positive correlations were observed among all investigated traits including the aging germination rate, relative aging germination rate, aging simple vigour index, aging primary root length, aging shoot length and aging total length. Thirteen QTL were identified to locate on five chromosome regions: Chr.1:297 Mb (chromosome 1 region 297 Mb), Chr.3:205 Mb, Chr.4:240 Mb, Chr.5:205 Mb and Chr.7:155 Mb, with 2 to 4 QTL co‐located on a region. In each region, 3–8 previously identified aging‐related QTL were located, confirming the importance of these regions for controlling seed longevity in different maize populations. Taken together, the results of this work provide a foundation for further QTL fine mapping and the molecular‐assisted breeding of aging tolerant maize. 相似文献
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10.
Inheritance of seed colour in Brassica campestris L. and breeding for yellow-seeded B. napus L. 总被引:3,自引:0,他引:3
Summary Seed colour inheritance was studied in five yellow-seeded and one black-seeded B. campestris accessions. Diallel crosses between the yellow-seeded types indicated that the four var. yellow sarson accessions of Indian origin had the same genotype for seed colour but were different from the Swedish yellow-seeded breeding line. Black seed colour was dominant over yellow. The segregation patterns for seed colour in F2 (Including reciprocals) and BC1 (backcross of F1 to the yellow-seeded parent) indicated that the black seed colour was conditioned by a single dominant gene. Seed colour was mainly controlled by the maternal genotype but influenced by the interplay between the maternal and endosperm and/or embryonic genotypes. For developing yellow-seeded B. napus genotypes, resynthesized B. napus lines containing genes for yellow seed (Chen et al., 1988) were crossed with B. napus of yellow/brown seeds, or with yellow-seeded B. carinata. Yellow-seeded F2 plants were found in the crosses that involved the B. napus breeding line. However, this yellow-seeded character did not breed true up to F4. Crosses between a yellow-seeded F3 plant and a monogenomically controlled black-seeded B. napus line of resynthesized origin revealed that the black-seeded trait in the B. alboglabra genome was possibly governed by two independently dominant genes with duplicated effect. Crossability between the resynthesized B. napus lines as female and B. carinata as male was fairly high. The sterility of the F1 plants prevented further breeding progress for developing yellow-seeded B. napus by this strategy. 相似文献
11.
Quantitative trait loci mapping of freezing tolerance and photosynthetic acclimation to cold in winter two‐ and six‐rowed barley
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Photoacclimation (PA) and freezing tolerance (FT) have been identified as closely related traits, due to common mechanisms of environmental control. In this study, diversity array technology (DArT) was used for identification of the quantitative trait loci (QTL) of FT and PA in winter barley. Simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers were subsequently used to saturate QTL regions. Two F2 mapping populations were created, for two‐rowed (P44) and six‐rowed barley (CaP). Different regions of the genome were responsible for differences in traits between parents in these two populations. Eleven QTLs were identified in the P44 population, including five typical for FT and PA, on chromosomes 2H, 3H and 7H. In the CaP population, only one QTL connected with PA and 10 connected with FT were found on all chromosomes except 2H. Our results demonstrate that different sets of markers should be applied in marker‐assisted selection for FT in two‐ and six‐rowed barley, as several loci determine FT at the level of biparental crosses. 相似文献
12.
Identification of quantitative trait loci underlying soybean (Glycine max [L.] Merr.) seed weight including main,epistatic and QTL × environment effects in different regions of Northeast China
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Seed weight (SW) is the important soybean (Glycine max [L.] Merr.), yield component and also affected the quality of soybean‐derived foods. The aim of this study was to identify the quantitative trait loci (QTL) underlying SW through 112 recombinant inbred lines (RILs) derived from the cross between “Zhongdou27” (G. max, designated by its bigger seed size, 21.9 g/100 seeds) and “Jiunong 20” (G. max, smaller seed size, 17.5 g/100 seeds). Phenotypic data were collected from this RIL population after it was grown in the sixteen tested environments. A total of eight QTL (QSW1‐1, QSW2‐1, QSW2‐2, QSW5‐1, QSW15‐1, QSW17‐1, QSW19‐1 and QSW20‐1) were identified, and they could explain 4.23%–14.65% of the phenotypic variation. Among these eight QTL, three QTL (QSW1‐1 located on the interval of Sat_159‐Satt603 of chromosome (Chr) 1 (LGD1a), QSW19‐1 located on the interval of Sat_340‐Satt523 of Chr 19 (LGL) and QSW20‐1 located on Sat_418‐Sat_105 of Chr 20 (LGI)) were newly identified and could explain 4.235%–10.08%, 8.45%–13.49% and 8.08%–10.18% of the phenotypic variation, respectively. Six of the eight identified QTL including QSW2‐2, QSW5‐1, QSW15‐1, QSW17‐1, QSW19‐1 and QSW20‐1 exhibited a significant additive (a) effect, while two QTL (QSW2‐1 and QSW19‐1) only displayed significant additive‐by‐environment (ae) effects. A total of four epistatic pairwise QTL for SW were identified in the different environments. These eight QTL and their genetic information obtained here were valuable for molecular marker‐assisted selection and the realization of a reasonable SW breeding programme in soybean. 相似文献
13.
甘蓝型油菜产量及相关性状的QTL分析 总被引:11,自引:1,他引:11
高产是甘蓝型油菜育种的重要目标之一,产量是多基因控制的数量性状。本文通过QTL作图分析了产量及其相关性状的数量性状位点,以甘蓝型油菜中油821和保604 F1代小孢子培养获得的DH系为作图群体,构建了由20个连锁群组成的,包括251个分子标记( 2个RFLP标记,72个RAPD标记,91个SSR标记,86个SRAP标记)的遗传连锁图(10个标记没有分配到连锁群中)。图谱的平均图距为6.96 cM,共覆盖油菜基因组1 746.5 cM。在此图谱基础上采取复合区间作图法,检测到与油菜产量及其相关性状有关的QTL共17个。其中控制株高的3个分别位于第4、第9和第10连锁群上,对性状的解释率为9.42%~17.58%;与分枝部位有关的4个分别位于第4、第6和第7连锁群上,其中Bp1 和Bp2 均位于第4连锁群,对性状的解释率为8.13%~15.20%;与主花序有效长有关的3个分别位于第4、第10和第16连锁群上,对性状的解释率为7.49%~23.36%;与一次有效分枝有关的2个分别位于第1、第4连锁群上,对性状的解释率为15.29%~19.58%;与角果总数和千粒重有关的分别位于第4连锁群和第9连锁群上,贡献率分别为17.42%和7.64%;与单株产量有关的3个分别位于第3、第4和第15连锁群,共解释26.60%的表型变异。部分性状的QTL在连锁群上成簇分布,对性状贡献率很大,表现主效QTLs的特点,相应的性状之间也呈显著相关,这表明一因多效或者相关的QTLs之间紧密连锁是性状相关的遗传基础。本研究中与主效QTLs连锁的标记可用于油菜产量性状的分子标记辅助选择。 相似文献
14.
Potato breeding aims at breeding diversified cultivars not only suitable for different purposes, but also resistant to diseases, such as late blight caused by Phytophthora infestans , which is a major constraint in potato production. Resistance to P. infestans has been previously introduced into the diploid hybrid population 98-21 from Solanum verrucosum and Solanum microdontum . In the present study, we assessed the segregation of tuber dormancy, tuber shape, regularity of tuber shape, eye depth and flesh colour in this population. Quantitative Trait Loci (QTLs) affecting these important quality traits were tagged using the genetic map developed for this population to locate QTLs for late blight resistance. The most prominent QTL for dormancy was detected on chromosome II and explained 7.1% of the variance. The most important QTLs for tuber eye depth, flesh colour, shape and shape regularity were identified on chromosomes X ( R 2 = 14.7%), IV ( R 2 = 5.8%), II ( R 2 = 8.0%) and III ( R 2 = 10.4%) respectively. All traits were also affected by minor QTLs. The obtained results improve our understanding of the inheritance of traits relevant for variety development in potato. 相似文献
15.
Hidetoshi Ajisaka Yasuhisa Kuginuki Susumu Yui Sueo Enomoto Masashi Hirai 《Euphytica》2001,118(1):75-81
DNA markers linked to a locus controlling an extreme late bolting trait, which was originally found in a local cultivar of
a non-heading leafy vegetable,‘Osaka Shirona Bansei’ (Brassica rapa L. ssp. pekinensis syn. campestris L.) were identified using bulked segregant analysis. A doubled haploid (DH) line, DH27, which is a progeny of ‘Osaka Shirona
Bansei’, shows extreme late bolting, and bolts without vernalization. DH27 was crossed with a normal bolting DH line, G309.
The plantlets of the parents, F1 and F2, were vernalized and then grown in a greenhouse. The bolting time of F2 plants showed a continuous distribution from 19 to 231 days after vernalization (DAV), suggesting the effects of a few major
genes and polygenes. Possible linkage markers for this trait were screened by modified bulked segregant analysis (BSA). The
BSA using four bulks suggested that a 530-bp RAPD band RA1255C was linked to a locus controlling the bolting trait. The RAPD
band was cloned and used as a probe to detect RFLP. The fragment detected a single locus, BN007-1,the segregation of which
in the F2 population matched that of RA1255C. Three other RAPDs were found to be linked to BN007-1. A quantitative trait locus(QTL)
affecting the bolting time was detected around BN007-1 using MAPMAKER/QTL. Since the difference between bolting times of both
the parental genotypes in the F2 was 138 days, these markers may be useful for a marker-assisted selection (MAS) in the breeding program for late bolting
or bolting-resistant cultivars in B. rapa crops.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
16.
B. Tar'an T. Warkentin D.J. Somers D. Miranda A. Vandenberg S. Blade D. Bing 《Euphytica》2004,136(3):297-306
Breeding efforts to improve grain yield, seed protein concentration and early maturity in pea (Pisum sativum L.) have proven to be difficult. The use of molecular markers will improve our understanding of the genetic factors conditioning these traits and is expected to assist in selection of superior genotypes. This study was conducted to identify genetic loci associated with grain yield, seed protein concentration and early maturity in pea. A population of 88 recombinant inbred lines (RILs) that was developed from a cross between 'Carneval' and 'MP1401' was evaluated at 13 environments across the provinces of Alberta, Manitoba and Saskatchewan, Canada in 1998, 1999 and 2000. A linkage map consisting of 193 AFLPs (amplified fragment length polymorphism), 13 RAPDs (random amplified polymorphic DNA) and one STS (sequence tagged site) marker was used to identify putative quantitative trait loci (QTL) for grain yield, seed protein concentration and early maturity. Four QTL were identified each for grain yield and days to maturity, and three QTL were identified for seed protein concentration. A multiple QTL model for each trait showed that these genomic regions accounted for 39%, 45% and 35% of the total phenotypic variation for grain yield, seed protein concentration and days to maturity, respectively. The consistency of these QTL across environments and their potential for marker-assisted selection are discussed in this report. 相似文献
17.
Fine mapping of qSS‐9, a major and stable quantitative trait locus,for seed storability in rice (Oryza sativa L.)
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Qiuyun Lin Yimei Jiang Ailing Sun Penghui Cao Linfang Li Xi Liu Yunlu Tian Jun He Shijia Liu Liangming Chen Ling Jiang 《Plant Breeding》2015,134(3):293-299
Seed storability in rice (Oryza sativa L.) is an important agronomic trait. We previously showed a quantitative trait locus of seed storability, qSS‐9, on chromosome 9 in a backcross population of ‘Koshihikari’ (japonica) / ‘Kasalath’ (indica) // ‘Koshihikari’. In this study, fine mapping of the chromosomal location of qSS‐9 was performed. Effect of ‘Kasalath’ allele of qSS‐9 was validated using a chromosome segment substitution line, SL36, which harboured the target quantitative trait loci (QTL) from ‘Kasalath’ in the genetic background of ‘Nipponbare’ under different ageing treatments in different environments. Subsequently, an F2 population from a cross between ‘Nipponbare’ and SL36 was used for fine mapping of qSS‐9. Simultaneously, four subnear isogenic lines (sub‐NILs) that represented different recombination breakpoints across the qSS‐9 region were developed from F3 progeny. Finally, the qSS‐9 locus was located between the Indel markers Y10 and Y13, which delimit a region of 147 kb in the ‘Nipponbare’ genome. These results provide a springboard for map‐based cloning of qSS‐9 and possibilities for breeding rice varieties with strong seed storability. 相似文献
18.
Although the Advanced Backcross strategy has proven very useful for QTL detection in tomato, it has been used mainly in identifying
QTL for agronomic traits such as yield, color, etc. Tomato flavor is an important quality characteristic, yet it has been
difficult to assess flavor or traits that affect it. In this study the AB-QTL strategy was applied to four advanced backcross
populations to identify QTL for biochemical properties that may contribute to the flavor of processed tomatoes, such as sugars
and organic acids. A total of 222 QTL were identified for 15 traits, including flavor as assessed by a taste panel. Correlations
of certain biochemicals with flavor and possible methods of assessing and improving flavor are discussed. In particular, QTL
with very significant effects associated with the ratio of sugars/glutamic acid, a trait highly correlated with improved flavor,
have been identified as good targets for future work in improving the flavor of tomatoes.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
19.
Summary Quantitative variability for seed yield and six other characters was analysed in Lotus corniculatus L. cv. Leo. The material consisted of 144 polycross progenies and 100 parents.Wide variability existed for all characters. The characters with the greatest variability were seed yield, forage grading and plant height. The polycross progeny test was employed to study the general combining ability of the parents. Highly significant differences existed for all seven characters under study.Parent-offspring genotypic and phenotypic correlations were high and significant for all characters except genotypic correlations for seed yield and seeds per pod. High h2 values (broad sense) were obtained for seed size and days to flowering. Traits with moderate to high h2 were seed yield (71% in parents, 64% in progenies), plant height, forage grading, and seeds per pod. The character pods per inflorescence had the lowest h2.Positive estimates of % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGak0dh9WrFfpC0xh9vqqj-hEeeu0xXdbba9frFj0-OqFf% ea0dXdd9vqaq-JfrVkFHe9pgea0dXdar-Jb9hs0dXdbPYxe9vr0-vr% 0-vqpWqaaeaabiGaaiaacaqabeaadaqaaqaaaOqaaiqabo8agaqcaK% aaavaabeqaceaaaeaacaqGYaaabaGaaeiraaaaaaa!3A89!\[{\text{\hat \sigma }}\begin{array}{*{20}c} {\text{2}} \\ {\text{D}} \\ \end{array} \] were obtained only for seed size. The ratio of dominance variance to additive variance indicated partial dominance for this character. Except for seed yield, in all other cases these estimates had very high sampling errors. In all cases except pods per inflorescence and seeds per pod high positive estimates of % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGak0dh9WrFfpC0xh9vqqj-hEeeu0xXdbba9frFj0-OqFf% ea0dXdd9vqaq-JfrVkFHe9pgea0dXdar-Jb9hs0dXdbPYxe9vr0-vr% 0-vqpWqaaeaabiGaaiaacaqabeaadaqaaqaaaOqaaiqbeo8aZzaaja% qbaeqabiqaaaqcaauaaiaaikdaaKaaGeaacaqGbbaaaaaa!3B30!\[\hat \sigma \begin{array}{*{20}c} 2 \\ {\text{A}} \\ \end{array} \] were obtained.The data indicated that it may be possible to simultaneously improve seed yield and maintain forage yield. Seed yield had positive and significant associations with seed size, seeds per pod and pods per inflorescence. The associations of days to flowering with forage grading (negative) and with pods per inflorescence (positive) were also significant. 相似文献