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一种由麦根腐平脐蠕孢(Bipolaris sorokiniana)引起的柳枝稷叶斑病 总被引:1,自引:0,他引:1
<正>柳枝稷(Panicum virgatum)属禾本科(Gramineae)黍属(Panicum)是多年生C4草本植物。该植物原产于北美,环境适应性极强,具有耐旱、耐盐碱、耐贫瘠等特点。它不仅是国际上公认的作为生物燃料的能源作物,还是一种优质牧草。截止2010年,柳枝稷在我国北方种植面积已达115.8 hm~2[1]。随着种植面积的不断扩大,柳枝稷病害问题越来越严重,病害造成柳枝稷生物量下 相似文献
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【目的】查明广西火龙果园病虫草害种类,探究火龙果主要病虫害的发生规律。【方法】火龙果病虫害种类调查采用了普查法和实地估测法,而主要病虫害的发生动态均采用五点取样调查法。【结果】调查表明,广西火龙果园的主要病虫草害及其天敌种类共有99种,其中害虫及有害动物有17种,病害有12种,杂草43种,昆虫天敌27种;在广西种植的火龙果品种中,金都1号、美龙、越南金边在同一年内发生3次炭疽病高发期,分别在3月、4月和7月,其中金都1号抗炭疽病性较好;溃疡病在同一年内出现2次高发期,分别在4月、7月,其中金都1号抗溃疡病较好;斜纹夜蛾、棉铃虫以及绿象甲在同一年内的种群数量变化均出现双峰型,年内2次高峰期发生在5月和7月,其中5月发生量较大。【结论】上述研究结果可提高人们对火龙果的病虫草害种类及主要病虫害发生规律的认识,为制订火龙果主要病虫草害综合防治方案奠定基础。 相似文献
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云南魔芋新病害—疫病病原菌的鉴定 总被引:2,自引:0,他引:2
<正>魔芋为天南星科(Araceae)魔芋属(Amorphophallus Blume)植物,是富含葡甘露聚糖的经济作物。随着人们对魔芋葡甘露聚糖的进一步研究和深度开发利用,魔芋的生产越来越受到重视,种植方式也由传统的半野生零星种植转向规模化大面积种植。云南是魔芋的起源中心之一[1],其气候环境非常适宜魔芋的生长和种植,但由于规模化种植程度的扩大以及芋农较为粗放的栽培管理方式,魔芋病害日趋严重,新病害亦不断出现。20世纪90年代初,我国魔芋有3种主要病害,现已增至11种。最近在云南省魔芋不同生育期的叶片及茎杆上又发现一种新病害——魔芋疫病。该病水渍状的黑 相似文献
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甘草根腐病病原菌鉴定 总被引:10,自引:0,他引:10
甘草(Glycyrrhiza uralensis Fish.)别名甜草、蜜草、甜根子, 为豆科多年生草本植物, 以根与根茎入药, 具有补脾益气、清热解毒、祛痰止咳、缓急止痛、调和诸药之功效, 是我国临床常用的中药材, 也可用作食品添加剂。甘草主要分布在我国的内蒙古、甘肃和宁夏, 在青海、陕西、新疆、黑龙江、辽宁、吉林、河北、山西等地局部地区也有分布。宁夏盐池县是我国乌拉尔甘草的重要产区, 面积大、贮量多、品质好, 1995年被誉为“中国甘草之乡” [1]。近年来, 野生甘草遭到了大规模采挖, 甘草蕴藏量急剧减少, 目前主要通过人工种植来满足市场需求。随着甘草种植面积的不断扩大, 甘草病虫害日趋严重, 根腐病危害尤为突出, 直接影响甘草的产量和品质, 造成巨大经济损失。 相似文献
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以分离自西瓜上的Aac5菌株为例,通过同源重组的方法,构建了hrc N基因插入缺失突变体,通过PCR方法和Southern blot验证突变菌株,对突变体进行致病性、致敏性、生长曲线和运动性测定。为明确hrc N基因与其他基因的关系,通过实时荧光定量PCR法定量检测了hrp A、hrc V、hrc U、Lux I、LuxR 5个基因的表达量。结果显示:与野生型相比,突变体致病力和致敏性明显减弱,致病时间延迟,群体感应信号减弱,生长能力明显下降,运动性减弱,互补菌株只能恢复部分功能;5个基因在突变体中的表达量均上调,hrc N基因与这5个基因之间均为负调控关系。说明hrc N基因在果斑病菌致病能力上发挥重要作用。 相似文献
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Gene S31pg1, which encodes a polygalacturonase (PG), was previously isolated from citrus race S31 of Geotrichum candidum, the causal agent of citrus sour rot. We have now isolated and sequenced an additional PG gene, S31pg2, with 95% identity to S31pg1 in the mature proteins. To evaluate the contribution of the two PG genes in the development of citrus sour rot, each gene was expressed in the fission yeast Schizosaccharomyces pombe. Both genes conferred PG activity to the yeast. Crude enzyme solutions containing S31PG1 severely degraded the albedo tissue of lemon peel, but those containing S31PG2 did not. Concentrated crude S31PG1 solutions also caused soft rot on lemon fruit, indicating that not S31PG2 but S31PG1 is an important pathogenicity factor in citrus sour rot. Next, the protopectinase (PP) activity of each PG was measured. Although S31PG1 and S31PG2 are highly homologous, S31PG1 had high PP activity, whereas S31PG2 had much lower activity. PG from G. candidum noncitrus race S63 (nonpathogenic to citrus fruits) was also assayed but did not have any PP activity at all. These results suggest that the different PP activities of the PGs are a key to the pathogenicity of G. candidum to lemon fruit. 相似文献
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三重PCR检测黄瓜炭疽病菌、菌核病菌和细菌性萎蔫病菌 总被引:1,自引:0,他引:1
本试验建立一种可同时检测黄瓜炭疽病(Colletotrichum orbiculare)、黄瓜菌核病(Sclerotinia sclerotiorum(Lib.)de Bary)和黄瓜细菌性萎蔫病(Erwinia tracheiphila)等黄瓜主要病害病原菌的三重PCR检测体系。采用正交试验设计方法, 对三重PCR的影响因素分析研究, 进行退火温度优化, 并以3个引物组、Taq DNA聚合酶、dNTP和Mg2+ 共6因素3水平进行多重PCR体系优化, 成功建立了适合黄瓜主要病害的三重PCR最佳检测体系, 即25 μL的反应体系中含有0.24 μmol·L-1 CY1/CY2;0.72 μmol·L-1 SSFWD/SSREV;0.336 μmol·L-1 ET-P1/ ET-P2;1 U Taq聚合酶;0.15 mmol·L-1 dNTP;1 mmol·L-1 MgCl2, 最适退火温度为63℃。该方法能够快速从田间黄瓜发病植株和根围土壤中将黄瓜炭疽病菌、黄瓜菌核病菌和黄瓜细菌性萎蔫病菌检测出来, 灵敏度可以达到10 pg·μL-1。 相似文献
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Satoshi Taba Nao Miyahira Kanami Nasu Tetsuya Takushi Zen-ichi Moromizato 《Journal of General Plant Pathology》2007,73(5):374-376
Strawberry pear (pitahaya, pitaya) [Hylocereus undatus (Haw.) Britt. and Rose] postharvest fruit rot was found at an agricultural products store in Itoman city, Okinawa Prefecture
in 2006. The symptoms included depressed, water-soaked lesions with olive to black powdery spots coalescing into a soft rot.
The causal fungus was identified as Bipolaris cactivora (Petrak) Alcorn. This is the first report of strawberry pear fruit rot caused by B. cactivora. 相似文献
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为明确取食马铃薯块茎和叶片的马铃薯块茎蛾Phthorimaea operculella幼虫肠道可培养细菌种类组成的多样性及其差异,分别对取食马铃薯块茎和叶片的马铃薯块茎蛾4龄幼虫的肠道细菌进行分离培养,根据菌落形态和生理生化特征及16S rDNA序列分析对菌株进行分类鉴定。结果表明,从取食马铃薯块茎的马铃薯块茎蛾肠道内共分离获得细菌有4门8科10属15种,其中褪色沙雷氏菌Serratia marcescen和乙酸钙不动杆菌Acinetobacter calcoaceticus为优势种,其相对多度分别为17.20%和16.06%。从取食马铃薯叶片的马铃薯块茎蛾肠道内共分离获得细菌有4门10科10属16种,其中琥珀葡萄球菌Staphylococcus succinus和乙酸钙不动杆菌为优势种,其相对多度分别为11.86%和15.07%。取食马铃薯块茎和叶片的马铃薯块茎蛾肠道内相同的可培养细菌有4属5种,分别为褪色沙雷氏菌、深红沙雷氏菌Serratia rubidaea、阿氏肠杆菌Enterobacter asburiae、乙酸钙不动杆菌和琥珀葡萄球菌。表明取食马铃薯块茎和叶片的马铃薯块茎蛾... 相似文献
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Kazutaka Kido Rie Adachi Masaru Hasegawa Kazutaka Yano Yasufumi Hikichi Shigeharu Takeuchi Tae Atsuchi Yuichi Takikawa 《Journal of General Plant Pathology》2008,74(4):302-312
An internal fruit rot with a malodor was found in netted melons (Cucumis melo L.) in commercial greenhouses in Kochi Prefecture, Japan, in 1998, despite their healthy appearance and lack of water-soaking
or brown spots on the surface. A yellow bacterium was consistently isolated from the affected fruits. To confirm the pathogenicity
of eight representative isolates of the yellow bacterium, we stub-inoculated ovaries (immature-fruits) 5–7 days after artificial
pollination, with a pin smeared with bacteria. After the melon fruits had grown for 60 more days, an internal fruit rot resembling
the natural infection appeared, and the inoculated bacterium was reisolated. The melon isolates had properties identical with
Pantoea ananatis, such as gram-negative staining, facultative anaerobic growth, indole production, phenylalanine deaminase absence, and acid
production from melibiose, sorbitol, glycerol, and inositol. Phylogenetic analysis based on 16S rDNA sequences showed that
the melon bacterium positioned closely with known P. ananatis strains. The melon bacterium had indole acetic acid (IAA) biosynthesis genes (iaaM and iaaH) and a cytokinin biosynthesis gene (etz). The bacterium could be distinguished from the other ‘Pantoea’ group strains by rep-PCR genomic fingerprinting. From these results, the causal agent of internal fruit rot was identified
as a strain of P.ananatis [Serrano in (Philipp J Sci 36:271–305, 1928); Mergaert et al. in (Int J Syst Bacteriol 43:162–173, 1993)].
The nucleotide sequence data reported are available in the DDBJ database under accessions AB297969, AB373739, AB373740, AB373741,
AB373742, AB373743 and AB373744. 相似文献
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大花蕙兰(Cymbidium hybridum),又名虎头兰、喜姆比兰,兰科、兰属多年生草本植物,是近几年我国花卉市场上流行的高档室内盆栽花卉。大花蕙兰的杂交育种已有一百多年的历史,每年新增加的品种有几十种之多。近年来,我国检验检疫部门分别从各入境口岸的进境大花蕙兰中多次发现携带有菊基腐病菌(Erwinia chrysanthemi)、兰花细菌性褐腐病菌(Erwinia cypripedii)、建兰花叶病毒(Cymbidium mosaic virus)等多种病原生物,均有潜伏期长、发病率高等特点。入境大花蕙兰种苗携带植物病原细菌、病毒等有害生物隐蔽性强、风险较高。 相似文献
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河南省玉米茎基部镰刀菌的形态和分子鉴定 总被引:7,自引:0,他引:7
为明确河南省玉米茎基部镰刀菌(Fusarium spp.)的种群组成及分布, 2011和2012年, 我们采集了河南省14个地市42个县(区)的玉米茎基腐病病样, 分离得到163个镰刀菌单孢菌株。首先对菌株进行了形态学鉴定, 在此基础上使用镰刀菌种特异性引物进行了PCR检测;对于部分PCR检测未能确认的菌株进行了Translation elongation factor 1-α(TEF-1α)基因片段测序及BLAST分析, 最终将163个菌株鉴定到种。结果表明在163个菌株中, 禾谷镰刀菌F. graminearum为优势种, 占44.2%(72株), 其次为层出镰刀菌F. proliferatum和轮枝镰刀菌F. verticillioides, 分别占28.8%(47株)和27.0%(44株)。豫西北的焦作(47.6%)、洛阳(50.0%)及豫中的许昌(45.5%)均以F. verticillioides为主, 豫东的商丘(42.9%)、开封(57.1%)以F. proliferatum为主, 豫北的安阳(66.7%)、濮阳(71.4%)、新乡(62.5%)和豫南的南阳(57.1%)、驻马店(45.0%)以及中部的郑州(57.1%)、漯河(66.7%)则均以F. graminearum为优势种。 相似文献
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Yukio Harada Shigeo Nakao Masahito Sasaki Yumi Sasaki Yukako Ichihashi Teruo Sano 《Journal of General Plant Pathology》2004,70(6):297-307
In 1982, an anamorphic fungus in the genus Monilia was first isolated as the causal agent of brown rot disease of Japanese apricot or mume (Prunus mume) in Oita Prefecture, Kyushu, Japan. Inoculation of flowers, shoots, and fruit of P. mume with the fungus reproduced brown rot disease symptoms similar to those found in nature. The fungus somewhat resembled the colony appearance of Monilinia (anamorph Monilia) laxa, the apricot brown rot fungus, on PSA plates, but it differed from the latter and the other two brown rot fungi, M. fructigena and M. fructicola, in terms of growth rate, temperature optima for mycelial growth and sporulation, morphology and germination pattern of conidia, nuclear number in the conidium, and nucleotide sequences in the ITS region of ribosomal DNA. It is newly described as Monilia mumecola Y. Harada, Y. Sasaki & T. Sano. A key to anamorphic states of four brown rot fungi of fruit trees is provided. 相似文献