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OBJECTIVE: To determine prevalence of paratuberculosis among dairy cattle herds and to identify associated soil-related risk factors. SAMPLE POPULATION: Serum and soil samples for 121 Michigan dairy herds. PROCEDURE: Blood samples were collected from cows at each farm and tested for Mycobacterium paratuberculosis, using an antibody ELISA. Soil samples were collected from pastures and exercise lots; pH and available iron content were determined. A questionnaire was administered to collect data regarding farm management practices and productivity. RESULTS: 55% of the herds tested had > or = 2 M paratuberculosis-positive cattle. Adjusting sample prevalence for distribution of herd size strata yielded a statewide herd prevalence of 54%. Of 3,886 cattle tested, 267 had positive results. Prevalence of test-positive cattle was 6.9%. For every part per million (ppm) increase in soil iron content, there was a 1.4% increase in the risk of a herd being test-positive. An increase in soil pH of 0.1 was associated with a 5% decrease and an increase in soil iron content of 10 ppm was associated with a 4% increase in the number of test-positive cattle. Application of lime to pasture areas was associated with a herd being only 10% as likely to be paratuberculosis positive and with a 72% reduction in number of test-positive cattle. CONCLUSIONS AND CLINICAL RELEVANCE: Prevalence of paratuberculosis-positive dairy herds in Michigan (54%) was greater than expected, but prevalence of paratuberculosis-positive cattle (6.9%) was within anticipated values. These prevalences were associated positively with acidic soil and increased soil iron content. Application of lime to pasture areas was associated with reduced risk of paratuberculosis. 相似文献
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Mee JF 《Veterinary journal (London, England : 1997)》2008,176(1):93-101
This review focuses on the case definition of dystocia, its current prevalence and recent temporal trends, the different types of dystocia and their associated risk factors in dairy cattle. The reported dystocia rates in dairy cattle internationally are generally <5%, apart from those in the United States, where they are higher. Given the skewed distribution of herd dystocia rates, average figures mask high prevalence herds. Phenotypic dystocia trends are generally increasing internationally and this trend has been partially attributed to the introduction of Holstein genes. The principal types of dystocia differ between primiparae and pluriparae, with feto-pelvic disproportion (FPD) predominating in the former and fetal malposition in the latter. In order of importance, the two major determinants of FPD are calf birthweight and maternal pelvic size. Abnormal fetal position is most influenced by the number of fetuses, parity and calf sire breed. Adequate weighting of dystocia in selection indices, achievement of heifer rearing targets prior to both service and calving, and appropriate periparturient management decisions are prerequisites for controlling dystocia in dairy cattle. 相似文献
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Scott HM Sorensen O Wu JT Chow EY Manninen K 《The Canadian veterinary journal. La revue veterinaire canadienne》2007,48(4):397-406
A province-wide cross-sectional seroprevalence and agroecological risk factor study of Mycobacterium avium subspecies paratuberculosis (MAP) and Neospora caninum (NC) infection among cattle in 100 cow-calf herds in Alberta was conducted. The seroprevalence of MAP in adult cattle was 1.5% across all herds. Using a widely accepted herd test cutpoint of 2 or more seropositive cows out of 30 animals tested, 7.9% of herds were estimated to be infected (95% confidence interval (CI): 2.3-23.4%). Seroprevalence of MAP differed by agroecological region; specifically, cattle and herds in areas with high soil pH (> 7.0), southern latitudes, and arid climates had a moderately reduced risk of infection (P < 0.10). Seroprevalence of NC infection was 9.7% among adult beef cattle province-wide--these levels also varied by agroecological region--with 91.0% of herds infected overall. 相似文献
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Antibodies to Coxiella burnetii have been found in the Danish dairy cattle population with high levels of herd and within herd seroprevalences. However, the prevalence of antibodies to C. burnetii in Danish beef cattle remains unknown. The objectives of this study were to (1) estimate the prevalence and (2) identify risk factors associated with C. burnetii seropositivity in Danish beef and dairy cattle based on sampling at slaughter. 相似文献
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Osterstock JB Fosgate GT Derr JN Cohen ND Roussel AJ 《Preventive veterinary medicine》2008,84(1-2):121-134
The objective of this study was to assess genetic similarity of beef cattle using microsatellite markers and to use this information to describe familial aggregation of paratuberculosis test results in Texas beef cattle. Paratuberculosis testing was performed on 2622 adult beef cattle using two commercially available serum ELISAs and radiometric fecal culture. Pedigree records were collected for registered purebred herds and herds with sufficiently detailed production records to identify parent-offspring pairs. Cases were defined as cattle with at least one positive paratuberculosis test result. Three controls were matched by herd of residence for each case. All parent-offspring pairs, cases, and controls were genotyped for 12 microsatellites. Bayesian analysis of allele frequency data was used to describe population substructure and assign individual cattle into groups of genetically similar cattle. The proportion of known parent-offspring pairs assigned to the same cluster was used to assess the validity of the approach to identify familial structure. Conditional logistic regression was used to describe the association between cluster assignment and paratuberculosis test-status matched by herd. Nine clusters of genetically similar individuals were identified and were supported by the proportion of parent-offspring pairs assigned to the same clusters. Increased odds of having at least one positive paratuberculosis test result were identified for two clusters compared to the cluster with the lowest proportion of positive paratuberculosis test results after conditioning on herd. The results of this study demonstrate that population substructure can be used to describe familial aggregation of paratuberculosis test results in beef cattle of unknown pedigree. 相似文献
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Pozzato N Capello K Comin A Toft N Nielsen SS Vicenzoni G Arrigoni N 《Preventive veterinary medicine》2011,102(1):83-86
Paratuberculosis is a chronic granulomatous infection caused by Mycobacterium avium subsp. paratuberculosis (MAP) that affects multiple ruminant species causing important economic losses. Therefore, control programmes at herd and regional levels have been established worldwide and prevalence estimates are needed for their implementation. Although different herd-level prevalence estimations for paratuberculosis have been reported in Europe, very few studies provided comparable and interpretable values, due to poor study designs and lack of knowledge about the accuracy of the diagnostic tests used. To overcome these problems we applied a latent class analysis to the results of two prevalence studies carried out in two neighbouring Northern Italian regions (Lombardy and Veneto) that account for over 50% of the Italian dairy cattle population. Serum samples from a randomly selected number of farms in the two regions were analyzed by different ELISA tests. The herd-level Apparent Prevalences (AP) were 48% (190/391) for Lombardy and 65% (272/419) for Veneto. Median within-herd APs were 2.6% and 4.0% for Lombardy and Veneto, respectively. Posterior estimates for the herd-level True Prevalences (TP) based on a Bayesian model were very similar between the two regions (70% for Lombardy and 71% for Veneto) and close to previous estimates of infected herds in Europe. The two 95% credibility intervals overlap each other, virtually showing only one distribution of the herd-level true prevalence for both regions. On the contrary, estimates of the within-herd TP distributions differed between the two regions (mean values: 6.7% for Lombardy and 14.3% for Veneto), possibly due to the different age distribution within the herds from the two regions. 相似文献
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Nyeleti C Hildebrandt G Kleer J Molla B 《Berliner und Münchener tier?rztliche Wochenschrift》2000,113(11-12):431-434
To estimate the prevalence and distribution of Salmonella in the chain from cattle to the consumer, faeces, mesenteric lymphnodes and beef cuts from 235 cattle, stool samples from 300 workers of the same Addis Ababa abattoir, and 330 minced beef samples from supermarkets in Addis Ababa were analyzed. Isolated Salmonella strains were serotyped and tested for antibiotic susceptibility. Low prevalence in faeces and lymphnodes, and higher contamination rates of beef cuts (diaphragm, abdominal muscles) indicate severe cross-contamination during slaughter. Animals of poor health status were far more frequently carriers of salmonellae, which stresses the need of intensive ante-mortem inspection on slaughter animals. During transport from slaughterhouse to the supermarkets, production and selling of minced beef, the prevalence of Salmonella did not increase. 相似文献
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Otranto D Llazari A Testini G Traversa D Frangipane di Regalbono A Badan M Capelli G 《Veterinary parasitology》2003,118(1-2):7-18
A cross-sectional serological survey for Neospora caninum was carried out on beef and dairy cattle in southern and northern Italy. A total of 111 herds and 1140 animals were tested using an ELISA assay (CHEKIT-Neospora) to detect anti-N. caninum antibodies. Management and individual data were collected and analysed both by linear and logistic multiple-regression models in order to find good predictors of the cattle seroprevalence and anti-N. caninum antibody level. At least one seropositive animal was found in 49 herds (44.1%), of which 31 (39.7%) from southern Italy and 18 (54.5%) from northern Italy. A total of 126 head of cattle (11%) were found to be seropositive and the seroprevalence was lower in southern (8.7%) than in northern Italy (16%). One of the best predictors of neosporosis seroprevalence in this study was the practice of self-rearing replacement heifers. Further risk factors were linked to higher stocking density, i.e. animals farmed in large herds and with no summer or permanent grazing practices were more likely to be seropositive than others. Farms with two or more dogs had higher herd seropositivity than farms with one or no dogs and this factor interacted significantly with the farm size and presence of poultry. Among individual characteristics, seropositivity was higher in animals sampled in mid- or late-pregnancy compared to animals either in early pregnancy or not pregnant. There was a significant interaction between the factors for pregnancy status and grazing practices. None of the epidemiological data recorded was a good predictor of the anti-N. caninum antibody level. 相似文献
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Geoffrey T. Fosgate Jason B. Osterstock Lisa A. Benjamin Georgina L. Dobek Allen J. Roussel 《Preventive veterinary medicine》2009,91(2-4):226-233
One thousand three hundred and twenty-four adult beef cattle were tested for paratuberculosis using 2 antibody enzyme-linked immunosorbent assays (ELISA), an interferon-gamma (INF-γ) ELISA, and radiometric bacterial culture of feces from 5 populations. Two populations of cattle (n = 226) had data available to calculate a ratio of humoral to cell-mediated immunity based on results from one antibody test and the INF-γ ELISA. Latent class analysis was used to estimate accuracy of the 4 paratuberculosis assays within a Bayesian framework. Determination of test accuracy and paratuberculosis prevalence in the latent class analysis allowed for estimation of predictive value positive (PVP) functions. The estimated PVP functions were used to iteratively assign paratuberculosis status to sampled cattle. Accuracy of the immunity ratio, an antibody ELISA, and the INF-γ ELISA were determined for multiple cutoffs based on probabilistically assigned paratuberculosis status. Area under the receiver-operating characteristic (ROC) curves (95% probability interval) were estimated as 0.78 (0.66, 0.89), 0.81 (0.68, 0.92), and 0.59 (0.47, 0.71) for the immunity ratio, antibody ELISA, and INF-γ ELISA, respectively. The Youden index (sensitivity + specificity − 1) peaked at immunity ratios of 0.5 (J = 0.48) and 1.0 (J = 0.46). Sensitivity and specificity (95% probability interval) at an immunity ratio cutoff of 0.5 were 0.65 (0.44, 0.85) and 0.83 (0.78, 0.88), respectively. Sensitivity and specificity (95% probability interval) at the 1.0 cutoff were 0.55 (0.33, 0.77) and 0.91 (0.87, 0.95), respectively. An immunity ratio could be used to diagnosis paratuberculosis in beef cattle but requires further investigation. 相似文献
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Osterstock JB Fosgate GT Cohen ND Derr JN Roussel AJ 《Journal of animal science》2008,86(8):1977-1983
A cross-sectional study was performed to determine the odds of having a positive paratuberculosis ELISA result if the dam was ELISA positive in Texas beef cattle, adjusted for individual and herd-level risk factors for seropositivity. Texas beef cattle (n = 2,621) were tested for paratuberculosis by using a commercial ELISA and microbiologic culture of feces for Mycobacterium avium subsp. paratuberculosis (MAP). Pedigree data were collected to identify dam-and sire-offspring pairs. Bayesian mixed-effects logistic regression was used to estimate the odds of seropositivity associated with age, dam ELISA status, sire ELISA status, herd size, herd history of clinical paratuberculosis, within-herd seroprevalence, within-herd fecal MAP prevalence, and within-herd fecal non-MAP Mycobacterium spp. prevalence. Herd of residence was included as a random effect to account for the correlation of observations within the same herd. Statistically probable associations were observed between ELISA status and herd fecal MAP prevalence [OR (odds ratio) 1.28 per 1% increase; P < 0.001] and herd seroprevalence (OR 1.21 per 1% increase; P < 0.001). The association with dam ELISA status was small (OR 1.35) and not highly probable (P = 0.69). Results indicate that use of dam ELISA status to make culling decisions in beef cattle may not improve the success of paratuberculosis control programs. Alternative strategies may be more effective for reducing the odds of seropositivity. 相似文献
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Cow and calf genetic and environmental factors were evaluated for their association with ELISA scores for paratuberculosis in a multibreed population of beef cattle. The ELISA scores are a measure of the presence or absence of antibodies against Mycobacterium avium subsp. paratuberculosis in bovine serum. The linear mixed-model analysis used 352 ELISA scores from 238 cows: 51 Angus (A); 34 Brahman (B); 41 (3/4 A 1/4 B); 45 (1/2 A 1/2 B); 34 (1/4 A 3/4 B); and 33 Brangus (5/8 A 3/8 B). Cows were assumed to be unrelated. Year affected (P < 0.001) ELISA scores, but age of cow did not, which was expected to be significant because of the chronic progressive nature of this disease. Important regressions on fixed effects associated with cows were 1) a positive estimate of cow B breed effect (0.59 +/- 0.24; P < 0.017), indicating an upward trend of ELISA scores toward 100% B cows; 2) a negative estimate for weight change from before calving (late November) to the date of the blood sample in May (-0.0062 +/- 0.0019 score/kg; P < 0.002), indicating that poorer maintenance of cow weights was associated with higher ELISA scores; and 3) a positive estimate for days in lactation of cow on the date of the blood sample (0.0086 +/- 0.0034 score/d; P < 0.021), indicating the production of larger amounts of antibodies against Mycobacterium avium subsp. paratuberculosis as lactation progressed. Relevant regressions on fixed effects associated with calves were 1) calf birth weight (-0.022 +/- 0.010 score/kg; P < 0.035), and 2) calf gain from birth to the date of the cow blood sample (-0.0092 +/- 0.0027 score/kg; P < 0.001). These estimates indicate that cows that produced lighter calves at birth and/or calves with slower preweaning growth tended to have greater ELISA scores. Although the sensitivity (percentage of infected animals detected) of ELISA was only 50%, these results suggest that subclinical paratuberculosis may be negatively affecting cows and their offspring. Factors identified as associated with ELISA scores could help producers with culling decisions related to paratuberculosis control and eradication in beef cattle. 相似文献
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Solis-Calderon JJ Segura-Correa VM Segura-Correa JC Alvarado-Islas A 《Preventive veterinary medicine》2003,57(4):199-208
The main objective of this cross-sectional study was to estimate the seroprevalence of infectious bovine rhinotracheitis (IBR) in a population of non-vaccinated beef cattle in the livestock region of Yucatan, Mexico and to determine potential risk factors related to the seroprevalence. Also, we estimated the intraherd correlation (re) and design effect (D) of IBR seropositivity. Cattle were selected by two-stage cluster sampling. Blood samples were collected from 564 animals from 35 herds. Sera were tested for antibodies against IBR using the serum-neutralisation test. Information regarding the herd and each animal sampled were recorded through a personal interview with the farmer or farm manager. The data were analysed using fixed-effects logistic multiple regression. Thirty-four of the 35 herds had at least one seropositive animal. The animal true seroprevalence was 54.4%. Animals in large herds or in production had higher odds of seropositivity than those in small herds or growing. The re and D were 0.17 and 3.62, respectively. 相似文献
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Tennent-Brown BS Pomroy WE Reichel MP Gray PL Marshall TS Moffat PA Rogers M Driscoll VA Reeve OF Ridler AL Ritaven S 《New Zealand veterinary journal》2000,48(5):149-150
AIM: To estimate the prevalence of Neospora infection in a sample of New Zealand beef cattle. METHODS: The prevalence of Neospora caninum infection in New Zealand beef cattle was estimated by collecting blood at slaughter from 499 beef cattle from 40 different farms at 2 slaughter plants in the North Island and 1 in the lower South Island . Sera were tested using an ELISA against Neospora tachyzoite antigen. RESULTS: The prevalence of seropositive cattle was 2.5% (n=120), 3.6% (n=166) and 2.3% (n=213) at the plants surveyed, the overall prevalence being 2.8%. The serologically positive cattle came from 9 farms, 3 of which had more than 1 positive animal. The highest prevalence recorded amongst animals from 1 farm was 4/13 (31%), in a group of young steers. CONCLUSION: Neosporosis appears to be present at a lower level in the New Zealand beef cattle population than in the New Zealand dairy cattle population. Nevertheless, from the high seroprevalence evident amongst young cattle on 1 farm, we suggest that Neospora may be a cause of infertility in beef cattle in this country. 相似文献
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B.S. Tennent-Brown W.E Pomroy M.P. Reichel P.L. Gray T.S. Marshall P.A. Moffat 《New Zealand veterinary journal》2013,61(5):149-150
dAim:To estimate the prevalence of Neospora infection in a sample of New Zealand beef cattle. dMethods: The prevalence of Neospora caninum infection in New Zealand beef cattle was estimated by collecting blood at slaughter from 499 beef cattle from 40 different farms at 2 slaughter plants in the North Island and 1 in the lower South Island. Sera were tested using an ELISA against Neospora tachyzoite antigen. dResults: The prevalence of seropositive cattle was 2.5% (n=120), 3.6% (n=166) and 2.3% (n=213) at the plants surveyed, the overall prevalence being 2.8%. The serologically positive cattle came from 9 farms, 3 of which had more than 1 positive animal. The highest prevalence recorded amongst animals from 1 farm was 4/13 (31%), in a group of young steers. dConclusion: Neosporosis appears to be present at a lower level in the New Zealand beef cattle population than in the New Zealand dairy cattle population. Nevertheless, from the high seroprevalence evident amongst young cattle on 1 farm, we suggest that Neospora may be a cause of infertility in beef cattle in this country. 相似文献
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Neospora caninum seroprevalence and associated risk factors in beef cattle in the northwestern United States 总被引:1,自引:0,他引:1
A Neospora caninum seroprevalence and risk factor survey of 2585 cows was conducted in 55 beef cow-calf herds located in five northwestern states of the USA. Blood samples were collected by private veterinary practitioners and management practices were surveyed using a mail questionnaire. Producers were randomly selected from those that employed these veterinarians to perform annual herd pregnancy examinations. Questions were asked about animal management, grazing and feeding, immunization and record keeping practices. Blood was collected from a systematically selected sample of cows in each herd, and age, origin, and pregnancy status were recorded. Blood samples were analyzed for antibodies against N. caninum antigen using a monoclonal antibody-based competitive inhibition (CI) ELISA. Overall seroprevalence was 24% and within herd seroprevalence ranged from 3 to 67% with a median of 19%. Within herd seroprevalence and mean inhibition percentage were different between the five states. Herds that managed their cows on range for summer grazing had lower seroprevalence than those that did not, while increased seroprevalence was associated with higher winter stocking density. Cows less than 3 years of age had higher CI ELISA inhibition percent values than cows greater than 6 years of age. No relationship was noted between serologic status and individual cow origin (purchased or raised), or pregnancy status at the time of sampling. 相似文献
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Boelaert F Walravens K Biront P Vermeersch JP Berkvens D Godfroid J 《Veterinary microbiology》2000,77(3-4):269-281
The national bovine paratuberculosis (PTB) seroprevalence (apparent prevalence) in the Belgian cattle population was determined by a serological survey that was conducted from December 1997 to March 1998. In a random sample of herds (N=556, 9.5%), all adult cattle of 24 months of age or older (N=13,317, 0.4%) were tested for the presence of antibodies using a commercially available absorbed ELISA test kit. The PTB median within-herd seroprevalence (proportion of detected animals within the seropositive herds) and the PTB individual-animal seroprevalence (proportion of detected animals) were, respectively, 2.9% (quartiles=1.6-5.6) and 0.87% (95% confidence interval (CI)=0.71-1.03). The PTB herd seroprevalence (proportion of detected herds) was 18% (95% CI=14-21).Assuming a test sensitivity and specificity of 45 and 99% [Sweeney et al., 1995. J. Vet. Diagn. Invest. 7 (4), 488; Sockett et al., 1992. J. Clin. Microbiol. 30 (5), 1134], respectively, the median true within-herd prevalence and the true individual-animal were estimated to be 7 and 2%, respectively. The true herd prevalence of Mycobacterium paratuberculosis infection was first estimated according to currently accepted methodology. This calculation revealed that the specificity of the used test has a dramatic effect on the estimation; assuming a test sensitivity of 45% and a true within-herd prevalence of 7%, the true herd prevalence estimation decreased from 36 to 0.8% if the test specificity decreased from 99. 9 to 99%, respectively. This sensitivity analysis showed that the practical limits of the accuracy of the used screening test jeopardize the estimation of the true herd prevalence within reasonable confidence limits, because the within-herd PTB true prevalence was low.For this reason we augmented the herd specificity for herds with larger adult herd size (>5). This was done by increasing the cut-off number of positive cattle required (>/=2) to classify a herd truly positive and including herds with one positive test result if there was historical evidence of PTB (previous diagnosis and/or clinical signs). This approach resulted in an estimated true herd prevalence of M. paratuberculosis infection of 6%. The true herd prevalence for dairy, mixed and beef herds was, respectively, 10, 11 and 3%. 相似文献
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Dargatz DA Byrum BA Hennager SG Barber LK Kopral CA Wagner BA Wells SJ 《Journal of the American Veterinary Medical Association》2001,219(4):497-501
OBJECTIVE: To estimate the prevalence of Mycobacterium avium subsp paratuberculosis infection among cows on beef operations in the United States. DESIGN: Cross-sectional seroprevalence study. Sample Population-A convenience sample of 380 herds in 21 states. PROCEDURES: Serum samples were obtained from 10,371 cows and tested for antibodies to M avium subsp paratuberculosis with a commercial ELISA. Producers were interviewed to collect data on herd management practices. RESULTS: 30 (7.9%) herds had 1 or more animals for which results of the ELISA were positive; 40 (0.4%) of the individual cow samples yielded positive results. None of the herd management practices studied were found to be associated with whether any animals in the herd would be positive for antibodies to M avium subsp paratuberculosis. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that the prevalence of antibodies to M avium subsp paratuberculosis among beef cows in the United States is low. Herds with seropositive animals were widely distributed geographically. 相似文献