UV-absorbing compounds and susceptibility of weedy species to UV-B radiation

Stratospheric ozone (O₃) depletion has led to increased terrestrial ultraviolet‐B (UV‐B) radiation (290–320 nm). Leaves exposed to this radiation produce UV‐absorbing compounds in the epidermal cells, which protect plants from UV‐B damage. To determine the role of UV‐absorbing compounds in the UV‐B sensitivity of weeds (common chickweed (Stellaria media), downy brome (Bromus tectorum), green smartweed (Polygonum scabrum), redroot pigweed (Amaranthus retroflexus), spotted cat’s‐ear (Hypochoeris radicata), and stork’s‐bill (Erodium cicutarium)) seedlings were exposed to 0, 4 (field ambient), 7 (18% O₃ depletion) and 11 (37% O₃ depletion) kJ m^?2 d^?1 of biologically effective UV‐B radiation in a greenhouse. Ultraviolet‐absorbing compounds were extracted from the second true‐leaf (0.5 cm² samples) with methanol : distilled water : HCl (79 : 20 : 1) in an 85°C water bath for 15 min, and the absorbance of the extracts measured at 300 nm. The shoot dry biomass was recorded to determine the susceptibility to UV‐B radiation. Common chickweed was the most sensitive and green smartweed the least sensitive weed to UV‐B radiation. The latter accumulated more UV‐absorbing compounds and this accumulation occurred earlier compared with common chickweed. As UV‐B_BE radiation levels increased from 0 to 11 kJ m^?2 d^?1, the green smartweed shoot biomass did not decline. However, the biomass of all five susceptible species declined despite an increase in the UV‐absorbing compounds in response to increased UV‐B radiation. Therefore, formation of a ‘UV‐screen’ in these species is not sufficient to fully prevent UV‐B damage. When the concentration of UV‐absorbing compounds in the six species was plotted against their susceptibility to UV‐B radiation, no relationship was observed. Thus, while the accumulation of UV‐absorbing compounds may be a major factor in the protection of certain species against UV‐B radiation and may offer some degree of defence in other species, it does not explain UV‐B susceptibility differences in weedy species in general.     

Solar UV-B radiation limits seedborne anthracnose infection and induces physiological and biochemical responses in Lupinus mutabilis

The aim of this study was to test whether solar UV-B radiation and temperature have an effect on infection of lupin seeds by Colletotrichum acutatum. Samples of infected seed were placed in a solar oven and exposed on sunny days for 15, 30, 45, 60, 75, 90 and 120 min. The degree of reduction in disease incidence and seed germination was dependent on the exposure time. Exposure times of 75 min (UV-B 4.41 kJ m⁻², ≈76 °C) and higher reduced incidence from 5% to undetectable levels, but also reduced seed germination by around 10% compared with untreated seed. Therefore, in a second experiment, infected seeds were exposed for 45 or 60 min in the solar oven (UV-B 2.83 or 3.75 kJ m⁻², respectively, ≈76 °C), for 60 min at ambient temperature (UV-B 3.75 kJ m⁻², ≈21 °C) or to dry heat for 60 min at 75 °C. Exposure for 60 min in the solar oven reduced seed infection by 99%, while UV-B-radiated seed at ambient temperature or dry-heat reduced infection by 60% or 32%, respectively. To evaluate the effect of UV-B plus high temperature on seedlings, lupin seed exposed for 45 or 60 min (UV-B 2.83 or 3.75 kJ m⁻², respectively, at ≈76 °C) were grown and physiological and biochemical responses of the seedlings were assessed. Seedlings from exposed seed had higher total concentrations of chlorophyll, protein and peroxidase activity than those grown from unexposed infected seed.     

The importance of reduced light intensity on the growth and development of six weed species

Crops limit light for photosynthesis and growth of weeds. We studied the effect of reduced light on performance of six weed species [one invasive species (Amsinckia micrantha), three common species (Veronica persica, Capsella bursa‐pastoris, Viola arvensis) and two less common weeds (Anagallis arvensis and Scleranthus annuus)]. In two glasshouse experiments, six light levels were achieved aiming at 0%, 20%, 50%, 80%, 90% and 95% reduction of light and corresponding with daily light integrals (DLI) of 12.4, 9.63, 7.13, 2.74, 0.95 and 0.69 mol m^?2 day^?1 in experiment 1 and 21.2, 18.0, 10.7, 3.71, 1.64 and 1.20 mol m^?2 day^?1 in experiment 2. The number of leaves was strictly controlled by DLI. Chlorophyll content index, maximum photochemical efficiency of photosystem II (F_v/F_m), stomatal conductance, flowering and dry matter were strongly reduced when DLI was reduced to 0.69–3.71 mol m^?2 day^?1 for all species. Threshold DLI for flowering was ca. 3.71 mol m^?2 day^?1 for S. annuus, V. arvensis, A. arvensis and V. persica, while C. bursa‐pastoris deviated by flowering at DLI of 0.95 mol m^?2 day^?1. This may explain why C. bursa‐pastoris is common in the seedbank of Danish arable soils in spite of intensive farming with well‐fertilised and dense crops.     

Characterization of triadimefon sorption in soils using supercritical fluid (SFE) and accelerated solvent (ASE) extraction techniques

Sorption–desorption of the fungicide triadimefon in field‐moist silt loam and sandy loam soils were determined using low‐density supercritical fluid extraction (SFE). The selectivity of SFE enables extraction of triadimefon from the soil water phase only, thus allowing calculation of sorption coefficients (K_d) at field‐moist or unsaturated conditions. Triadimefon sorption was influenced by factors such as soil moisture content and temperature; sorption increased with increased moisture content up to saturation, and decreased with increased temperature. For instance, K_d values for triadimefon on the silt loam and the sandy loam soils at 40 °C and 10% water content were 1.9 and 2.5 ml g⁻¹, respectively, and at 18% water content, 3.3 and 6.4 ml g⁻¹, respectively. Isosteric heats of sorption (ΔH_i) were −42 and −7 kJ mol⁻¹ for the silt loam and sandy loam soils, respectively. Sorption–desorption was also determined using an automated accelerated solvent extraction system (ASE), in which triadimefon was extracted from silt loam soil by 0.01 M CaCl₂. Using the ASE system, which is basically a fast alternative to the batch equilibration system, gave a similar ΔH_i value (−29 kJ mol⁻¹) for the silt loam soil (K_f = 27 µg^{1 − 1/n} ml^1/n g⁻¹). In order to predict transport of pesticides through the soil profile more accurately on the basis of these data, information is needed on sorption as a function of soil water content. © 2000 Society of Chemical Industry     

Seed structure and germination in Discaria toumatou (Rhamnaceae)

Data collected from field trials carried out in different years and environments were used to determine competitive ability and threshold levels of Ammi majus L., Chenopodium album L. and Sinapis arvensis L. The hyperbolic model showed a good fit to the experimental data. The three broadleaf weeds showed low and similar competitive indices (i ranging from 1–08 to 1–75), supporting the hypothesis of a high sunflower competitive ability. The economic threshold was four to six plants m^?2 for all three weeds in the case of weed control by hoeing and about six plants m^?2 for S. arvensis in the case of a postemergence treatment with imazamethabenz. Limitations and approximations in estimating competitive parameters are pointed out and discussed. Aptitude à la compétition et seuils de nuisibilité de trois mauvaises herbes dicotylédones dans le tournesol Des données recueillies dans des expérimentations au champ conduites dans différents environnements et au cours de différentes années ont été utilisées pour déterminer l'aptitude à la compétition et les seuils de nuisibilité de Ammi majus L., Chenopodium album L. et Sinapis arvensis L. Le modéle hyperbolique fournissait un bon ajustement aux données. Les trois mauvaises herbes avaient des indices de compétition bas et similaires (i de 1,08 à 1,75) ce qui conforte l'hypothèse d'une forte aptitude à la compétition du tournesol. Les seuils économiques étaient 4–6 plantes m^?2 pour toutes les mauvaises herbes dans le cas de désherbage mécanique et environ 6 plantes m^?2 pour S. arvensis dans le cas de traitement de post-levée avec l'imazaméthabenz. Les limitations et les approximations dans l'évaluation des paramètres de la compétition sont identifiés et discutés. Konkurrenzkraft und Schadensschwellen von drei dikotylen Unkrautarten in Sonnenblume Anhand von Ergebnissen aus Freilandversuchen in verschiedenen Jahren auf verschiedenen Standorten wurden die Konkurrenzkraft und die Schadensschwellen von Ammi majus L., Chenopodium album L., und Sinapis arvensis L, bestimmt. Die Ergebnisse ließen sich mit einer hyperbolischen Gleichung gut beschreiben. Die Konkurrenzindices i von 1,08 bis 1,75 waren fur alle 3 Arten ahnlich niedrig, worin sich die starke Konkurrenz der Sonnenblume widerspiegelt. Bei mechanischer Unkrautbekampfung lagen die Schadensschwellen fur alle 3 Unkrauter zwischen 4 und 6 Pflanzen m^?2, fur Nachauflaufbehandlung mit Imazametabenz fur Sinapis arvenis bei 6 Pflanzen m^?2. Grenzen und Annaherung der Bestimmung von Konkurrenzparametern werden eingehend diskutiert.     

Competitive ability and threshold levels of three broadleaf weed species in sunflower

Data collected from field trials carried out in different years and environments were used to determine competitive ability and threshold levels of Ammi majus L., Chenopodium album L. and Sinapis arvensis L. The hyperbolic model showed a good fit to the experimental data. The three broadleaf weeds showed low and similar competitive indices (i ranging from 1–08 to 1–75), supporting the hypothesis of a high sunflower competitive ability. The economic threshold was four to six plants m^?2 for all three weeds in the case of weed control by hoeing and about six plants m^?2 for S. arvensis in the case of a postemergence treatment with imazamethabenz. Limitations and approximations in estimating competitive parameters are pointed out and discussed. Aptitude à la compétition et seuils de nuisibilité de trois mauvaises herbes dicotylédones dans le tournesol Des données recueillies dans des expérimentations au champ conduites dans différents environnements et au cours de différentes années ont été utilisées pour déterminer l'aptitude à la compétition et les seuils de nuisibilité de Ammi majus L., Chenopodium album L. et Sinapis arvensis L. Le modéle hyperbolique fournissait un bon ajustement aux données. Les trois mauvaises herbes avaient des indices de compétition bas et similaires (i de 1,08 à 1,75) ce qui conforte l'hypothèse d'une forte aptitude à la compétition du tournesol. Les seuils économiques étaient 4–6 plantes m^?2 pour toutes les mauvaises herbes dans le cas de désherbage mécanique et environ 6 plantes m^?2 pour S. arvensis dans le cas de traitement de post-levée avec l'imazaméthabenz. Les limitations et les approximations dans l'évaluation des paramètres de la compétition sont identifiés et discutés. Konkurrenzkraft und Schadensschwellen von drei dikotylen Unkrautarten in Sonnenblume Anhand von Ergebnissen aus Freilandversuchen in verschiedenen Jahren auf verschiedenen Standorten wurden die Konkurrenzkraft und die Schadensschwellen von Ammi majus L., Chenopodium album L., und Sinapis arvensis L, bestimmt. Die Ergebnisse ließen sich mit einer hyperbolischen Gleichung gut beschreiben. Die Konkurrenzindices i von 1,08 bis 1,75 waren fur alle 3 Arten ahnlich niedrig, worin sich die starke Konkurrenz der Sonnenblume widerspiegelt. Bei mechanischer Unkrautbekampfung lagen die Schadensschwellen fur alle 3 Unkrauter zwischen 4 und 6 Pflanzen m^?2, fur Nachauflaufbehandlung mit Imazametabenz fur Sinapis arvenis bei 6 Pflanzen m^?2. Grenzen und Annaherung der Bestimmung von Konkurrenzparametern werden eingehend diskutiert.     

Methyl isothiocyanate volatilization from fields treated with metam‐sodium

Emission of methyl isothiocyanate (MITC) from fields treated with metam‐sodium (sodium N‐methyldithiocarbamate) is a potential environmental and human safety hazard. Concentrations of MITC at three heights above four arable fields were measured following the application of metam‐sodium at a rate of 480 litre ha⁻¹ (166 kg AI ha⁻¹). Two of these fields were treated by injection into a center‐pivot irrigation system (chemigation), while in the other two fields the fumigant was applied through injection directly into the soil. Generally, higher MITC air concentrations were observed above chemigated than above injected fields. Maximum MITC air concentrations were 11.2 and 7.4 µg m⁻³ recorded 10 cm above ground 6–8 h following application and the minimum concentrations were 0.7 and 0.2 µg m⁻³ observed at 200 cm 30–35 h after application above chemigated and injected fields, respectively. The estimated MITC respiratory exposure a worker might encounter during the re‐entry period ranged between 1.37 and 0.03 mg day⁻¹ in chemigated fields and between 0.35 and 0.02 mg day⁻¹ in the injected fields. These results suggest that application of the fumigant through injection reduced MITC volatilization losses in comparison with the chemigation method, thus posing a relatively lower risk of exposure to MITC emissions. © 2000 Society of Chemical Industry     

Competition between Imperata cylindrica and maize in the forest savannah transition zone of Nigeria

Imperata cylindrica is a noxious weed that infests annual and perennial crops in most tropical regions. High crop densities may offer opportunities to reduce I. cylindrica competition in small‐scale farming systems. The competitive ability of maize relative to I. cylindrica was evaluated in an addition series experiment in the forest savannah transition zone in 2006 and 2007 at Ibadan, Nigeria. Maize and I. cylindrica were planted in eight monoculture densities (4, 8, 12, 16, 20, 32, 48 and 64 plants m^?2) and in a 1:1 mixture at eight total densities (2:2, 4:4, 6:6, 8:8, 10:10, 16:16, 24:24 and 32:32 maize: I. cylindrica plants m^?2) as in monoculture. Non‐linear regression models were used to relate crop and weed shoot biomass to their densities and total grain yield to maize density. In maize, intraspecific competition was more than interspecific competition; in I. cylindrica, interspecific competition was higher than intraspecific. As expected, total grain yield was lower in the mixture than in maize monoculture at all total densities. Average maize grain yield in maize monoculture differed from that in mixtures by 0.77 t ha^?1 in 2006 and 0.57 t ha^?1 in 2007. Niche differentiation indices were <1 in 2006 and >1 in 2007, indicating that both species competed for similar resources in 2006, but not in 2007. The greater competitive ability of maize over I. cylindrica may be associated with rapid growth and canopy development observed in the field.     

Does soil nitrogen affect early competitive traits of annual weeds in comparison with maize?

Soil nitrogen (N) is considered an important driver of crop‐weed interactions, yet the mechanisms involved have been only partially explored, especially with respect to early‐season growth, when competitive hierarchies are formed. This study characterises the effects of different N levels on biomass accumulation and plant morphology for maize (Zea mays), and four important weed species (Amaranthus retroflexus, Abutilon theophrasti, Setaria faberi, and Chenopodium album). Under glasshouse conditions, plants were grown in separate pots and irrigated with nutrient solution at four N concentrations (0.2, 0.5, 2, 5 μm L⁻¹) until 57 days after emergence. Except for S. faberi, which was unresponsive to N, the relative biomass growth rates (RGR) of maize and the broad‐leaved weeds were positively and similarly affected by increasing nitrogen. At all N levels, maize had a height advantage by virtue of its larger seed size, which conferred early growth benefits independent of RGR. At low N, biomass growth was instrumental to S. faberi’s improved competitive position, whereas height development per unit biomass improved the competitive position of A. theophrasti, C. album and A. retroflexus. The approach presented could be applied to other crop‐weed systems to evaluate environmental impacts on competitive outcomes.     

Weed seed bank as affected by tillage,residue, and fertilization management under sweet corn-wheat cropping sequence in Iran

Soil weed seed bank is an important factor determining above-ground floristic composition and weed density in agricultural systems. The quantitative and qualitative measures of weed seed bank can help growers to predict the extent to which they are facing weed problems. Along with tillage, crop residues can affect the fate of weeds in the upcoming crops. To investigate such effects, we compared the effects of tillage systems [conventional tillage (CT), reduced tillage (RT), and no tillage (NT)], wheat residue retention, and nitrogen (N) rates (0, 69, 138, and 207 kg N ha⁻¹) on depth-related characteristics of the weed seed bank under a sweet corn-wheat sequence during 2014–2015 growing seasons in Shiraz, Iran. Soil bank was not affected by tillage systems but tended to be slightly higher under RT. The highest (898 seeds m⁻²) and lowest (322 seeds m⁻²) weed population at 0–10 cm depth were found when 138 kg N ha⁻¹ in 2015 and 207 kg N ha⁻¹ in 2014 were applied. Species richness and diversity were higher under NT and RT practices at the top layer, but CT system was more diversified at deeper depths. They were higher when crop residues were retained as well. Barnyard grass (Echinochloa crus-galli [L.] Beauv), common lambsquarter (Chenopodium album L.), common purslane (Portulaca oleracea L.), field bindweed (Convolvulus arvensis L.), flixweed (Descoreinia sofia [L.] Webb. & Berth.), henbit (Lamium amplexicaule L.), pigweeds (Amaranthus spp.), and stinking goosefoot (Chenopodium vulvaria L.) were the most common weeds found in all tillage systems and soil depths. Grasses were relatively lower than broadleaves regardless of treatments. Weed seed bank was mostly affected by weather conditions than treatments in this short-term experiment.     

Effect of air temperature, rain and drought on hot water weed control

The influence of rain and drought before, and air temperature during, weed control with hot water was studied in laboratory experiments on the test weed Sinapis alba (white mustard). The plants were grown in a greenhouse and treated outdoors. There was no difference in weed control effect when S. alba plants at the four‐leaf stage were treated at the air temperatures 7°C and 18^°C. The effective energy dose for a 90% fresh weight reduction was 465 kJ m^?2 for both air temperatures. Weed control of S. alba at the four‐ to six‐leaf stage in rainfall above the rainwater run‐off level increased the required effective energy dose by 20% (i.e. 120 kJ m^?2) compared with dry plants. A short period of drought just before treatment on S. alba at the two‐ to four‐leaf stage increased the plant fresh weight reduction, which was 22% at low energy dose (190 kJ m^?2) and 44% at high energy dose (360 kJ m^?2). Hot water weed control should thus be carried out when the plants are drought stressed and avoided when the plants are wet. The air temperature seems to be of little importance in the range 7–18^°C.     

Effects of different potato cultivars on life history and demographic parameters of Phthorimaea operculella Zeller (Lepidoptera: Gelechiidae)

Potato tuber worm, Phothorimae operculella Zeller (Lepidoptera: Gelechiidae) is a common pest of potato which causes a heavy loss in yield either in fields or storage. The effect of six potato cultivars (Agria, Florida, Impala, Picasso, Satina, and Sprint) were evaluated on the life history, life table and demographic parameters of P.operculella under laboratory conditions, which could be appropriate indices in resistance and susceptibility evaluation of potato cultivars. The longest development time was on Picasso (28.76 ± 0.36d) cultivar. The male longevity ranged from 4.83 ± 0.44d on Sprint to 7.45 ± 0.60d on Picasso. Impala and Satina with 53.22 ± 7.78 and 28.74 ± 3.54 eggs/individual had the highest and the lowest net reproductive rates (R₀). The highest values for the intrinsic rate of increase (r_m) and finite rate of increase (λ) were on Impala (r_m: 0.119 day^?1, λ: 1.75 day^?1), and the lowest value was on Sprint (r_m: 0.090 day^?1, λ: 1.01 day^?1). The results indicated that Sprint was the most resistant cultivar for the feeding of P.operculella, which could be useful in the development of integrated pest management programs for this pest.     

The mode of action of RH‐1965: a new phenylpyrimidinone bleaching herbicide

RH‐1965 is a new bleaching herbicide which causes newly developing leaf tissue to emerge devoid of photosynthetic pigments. Mode‐of‐action studies revealed that RH‐1965 inhibited the accumulation of both total chlorophyll and β‐carotene. Concomitantly, it induced the accumulation of the β‐carotene precursors, phytoene, phytofluene and, in particular, ξ‐carotene. Inhibition of chlorophyll accumulation by RH‐1965 is attributed to the photo‐oxidative destruction of chlorophyll in the absence of β‐carotene because RH‐1965 blocked chlorophyll accumulation to a greater extent under high light (50–330 µE m⁻² s⁻¹) than under low light (0.8 µE m⁻² s⁻¹) conditions. Radish (Raphanus sativus L) and barnyardgrass (Echinochloa crus‐galli (L) Beauv) were very senstive to RH‐1965. Under high light (330 µE m⁻² s⁻¹), the I₅₀ values for inhibition of chlorophyll accumulation were 0.10 and 0.15 µM , respectively. Wheat (Triticum aestivus L), on the other hand, was much less sensitive to RH‐1965 (I₅₀ = 1.4 µM ). It is concluded that the mode of action of RH‐1965 involves the inhibition of ξ‐carotene desaturation. © 2000 Society of Chemical Industry     

Competitive interactions between chick-pea genotypes and weeds

Growing chick‐pea in sustainable systems requires the use and development of more competitive genotypes which can complement the effects of reduced input weed control. A 2‐year study assessed the competitive ability of 13 genotypes grown in either the presence or absence of weeds, in a split‐plot design including the weeds in pure stands. Crop and weed density, phenology, relative biomass of crop (RB_c) and weeds (RB_w), crop yield characters, crop biometric traits in the absence of weeds, relative biomass total of mixtures (RBT) and crop competitive ability (C_b = ln RB_c/RB_w) were recorded. Lines C136, C120, C101 and C106, and cultivars Pascià, Visir and Sultano gave the best seed yield in the absence of weeds (1.8–2.0 t ha^?1 DM). Weeds reduced yield by 75% and 83% in C136 and C133 and by 87–97% in the other genotypes. Weed biomass in mixture (mainly Chenopodium album) averaged 4.42 t ha^?1 DM. Chick‐pea genotypes C136 and C133 were the most competitive, but weeds were more competitive than any of the chick‐peas. C_b was correlated directly to the height of first fertile pod (r² = 0.84) and inversely to the insertion angle of primary branches to the vertical (r² = 0.77). Intergenotypic variation for competitive ability could be exploited in integrated weed control using more competitive genotypes, or used in breeding programmes aimed to develop highly competitive cultivars on the basis of easily screenable characters.     

Effects of temperature and concentration of the accelerators ethoxylated alcohols,diethyl suberate and tributyl phosphate on the mobility of [14C]2,4‐dichlorophenoxy butyric acid in plant cuticles

Intrinsic activities of monodisperse ethoxylated dodecanols (MEDs), diethyl suberate (DESU) and tributyl phosphate (TBP) were investigated using Stephanotis floribunda leaf cuticular membranes (CMs) and [¹⁴C]2,4‐dichlorophenoxy butyric acid (2,4‐DB) as a model solute. When sorbed in cuticular membranes, MEDs, DESU and TBP increase solute mobility and are called accelerators for this reason. With MEDs, dose‐effect curves (log mobility vs accelerator concentration) were linear but, with DESU and TBP, curves convex to the x axes were obtained that approached a maximum at 90 and 150 g kg⁻¹, respectively. Accelerators increased the mobility of 2,4‐DB in the CMs by 9‐ to 48‐fold, and effects were larger at lower temperatures (range 15–30 °C). Activation energy for diffusion of 2,4‐DB was 105 kJ mol⁻¹, decreasing with increasing accelerator concentrations to 26 kJ mol⁻¹ with DESU at 90 g kg⁻¹ and 64 kJ mol⁻¹ with TBP at 150 g kg⁻¹. Thus, the intrinsic activity of DESU was much higher than that of TBP, which implies that, for a given effect, less DESU than TBP would be needed. MEDs were also very effective accelerators, lowering activation energies to 36 kJ mol⁻¹. Data are discussed in relation to increasing rates of foliar penetration of active ingredients at low temperatures. © 2001 Society of Chemical Industry     

Concentration-Dependent Mobility of Chlorfenvinphos in Isolated Plant Cuticles

The mobility of chlorfenvinphos in isolated pear (Pyrus communis cv. Bartlett) leaf cuticular membranes (CM) was studied as a function of concentration of chlorfenvinphos sorbed in the cuticle. Mobilities of chlorfenvinphos increased approximately 9-fold when the amount sorbed increased from 1 to 100 μg cm⁻² pear leaf cuticle. From the amounts per area, average volume fractions of chlorfenvinphos in the cuticle were calculated ranging from 2×10⁻³ to 5·1×10⁻². The increase in mobilities was steepest at the lower and levelled off at higher volume fractions. This correlation could be described for the whole range of volume fractions investigated by an equation which assumes homogeneously dispersed chlorfenvinphos. Temperature dependence of mobilities was studied at 17, 25 and 35°C and chlorfenvinphos volume fractions of 5·5×10⁻³ and 0·12, respectively. Arrhenius graphs were linear for both volume fractions, showing that cuticles did not undergo a phase transition due to the high amount of sorbed chlorfenvinphos. However, at a volume fraction of 0·12, the activation energy of diffusion, E_D, was significantly lower (83·6 kJ mol⁻¹) than at 5·5×10⁻³ (135 kJ mol⁻¹). We interpret these findings as evidence for a plasticising effect on cuticular waxes by chlorfenvinphos. So far, such an effect had been demonstrated only for certain adjuvants (ethoxylated alcohols) but not for active ingredients. Chlorfenvinphos not only increased its own mobility in pear leaf cuticles, but also that of 2,4-D in Citrus leaf cuticles. This would be expected if plasticising of waxes was the sole mechanism responsible for increased mobilities. From these data we predict that permeabilities of cuticles to chlorfenvinphos are not constant. Depending on temperature as well as types and amounts of adjuvants, rates of foliar penetration of chlorfenvinphos can be higher if its concentration in the spray liquid is increased.     

Photolytic versus microbial degradation of clomazone in a flooded California rice field soil

BACKGROUND: Clomazone is a popular herbicide used on California rice fields and exhibits rapid anaerobic microbial degradation (t_1/2 = 7.9 days). To test the potential of direct and indirect photolytic degradation as a cofactor in the overall degradation rate, sacrificial time‐series microcosms were amended with water, non‐sterilized soil + water and sterilized soil + water. Clomazone was added to each microcosm, which was then exposed to natural and artificial sunlight over 35 days. Water and acetonitrile extracts were analyzed for clomazone and metabolites via LC/MS/MS. RESULTS: The calculated pseudo‐first‐order degradation rate constants (k) were k_water = 0–0.005 ± 0.003 day^?1, k_sterile = 0–0.005 ± 0.003 day^?1 and k_non?sterile = 0.010 ± 0.002–0.044 ± 0.007 day^?1, depending on light type. The formation of ring‐open clomazone, a microbial metabolite, correlated with clomazone degradation. Trace amounts of 5‐hydroxyclomazone (m/z = 256 → 125), aromatic hydroxyclomazone (m/z = 256 → 141) and an unknown product (m/z = 268 → 125) were observed. CONCLUSIONS: The photolytic degradation rate depends on both light type and the quality of the chromophores that induce indirect photolysis. Microbial degradation was found to be sensitive to temperature fluctuations. Overall, microbes are shown to be more detrimental to the environmental fate of clomazone than photolysis. Copyright © 2012 Society of Chemical Industry     

Germination response of Orobanche seeds subjected to conditioning temperature, water potential and growth regulator treatments

Broomrapes (Orobanche spp.) are parasitic weeds that cause significant losses of crop yield. Experiments were conducted to investigate the seed response to the artificial germination stimulant GR₂₄ in three species of Orobanche subjected to preconditioning under various temperatures, water potentials and with plant growth regulators. The highest germination percentages were observed in Orobanche ramosa, Orobanche aegyptiaca and Orobanche minor seeds conditioned at 18°C for 7 days followed by germination stimulation at 18°C. With the increase of the conditioning period (7, 14, 21 and 28 days), the germination percentage of O. ramosa and O. aegyptiaca progressively decreased. When conditioned at −2 MPa, the germination percentage was lower than at 0 and −1 MPa, especially at 13 and 28°C. Orobanche minor seeds could retain relatively high germination if conditioned at 18, 23 or 28°C, even after significantly extended conditioning periods (up to 84 days). GA₃ (30–100 mg L⁻¹), norflurazon and fluridone (10–100 mg L⁻¹), and brassinolide (0.5–1.0 mg L⁻¹) increased seed germination, while 0.01 mg L⁻¹ uniconazole significantly reduced germination rates of all three Orobanche spp. The promotional effects of GA₃ and norflurazon and the inhibitory effect of uniconazole were evident, even when they were treated for 3 days. Germination of Orobanche seeds was much lower when the unconditioned seeds were directly exposed to GR₂₄ at 10⁻⁶ m . This early GR₂₄-induced inhibition was however alleviated or even eliminated by the inclusion of GA₃ or norflurazon (10–50 mg L⁻¹) in the conditioning medium. On the contrary, the inclusion of uniconazole increased the inhibitory effect of GR₂₄, particularly in the case of O. ramosa.     

Residues in apples and sweet cherries after methyl bromide fumigation

Methyl bromide fumigations are used to treat apples, Malus domestica Borkh, and sweet cherries, Prunus avium (L), before export to Japan. In order to expand existing markets, additional cultivars are being prepared for export to Japan. As part of the approval process, residue analyses must be conducted and residues must be at acceptable levels. Five apple cultivars (‘Braeburn,’ ‘Fuji,’ ‘Gala,’ ‘Jonagold,’ and ‘Granny Smith’) were fumigated at 40 g m⁻³ for 2 h at 10 °C, and six sweet cherry cultivars (‘Brooks,’ ‘Garnet,’ ‘Lapin,’ ‘Rainier,’ ‘Sweetheart,’ and ‘Tulare’) were fumigated for 2 h with 64 g m⁻³ at 6 °C, 48 g m⁻³ at 12 °C, 40 g m⁻³ at 17 °C, and 32 g m⁻³ at 22 °C. Three replicates of fruit from each fumigation were analyzed for methyl bromide and bromide ion residues periodically with time. Methyl bromide residues for both apples and cherries were the highest immediately after fumigation, but rapidly declined so that only ‘Braeburn’ had residues >8 µg kg⁻¹ after 13 days and, except for ‘Lapin,’ all cherries were <1 µg kg⁻¹ after seven days. Average bromide ion residues were between 3.3 and 4.9 mg kg⁻¹ among apple cultivars, and between 3.7 and 8.0 µg kg⁻¹ among cherry cultivars. Published in 2000 for SCI by John Wiley & Sons, Ltd