Resistances of grasses to two sobemoviruses, cocksfoot mottle and cynosurus mottle

Infection of Dactyls glomerata with cocksfoot mottle virus (Cf MV) and of Cynosurus cristatus with cynosurus mottle virus (CyMV) is frequently lethal. However, varieties of each species differ in the proportion of genotypes which survive, and in the damage suffered by these surviving plains. Infected plants of the CfMV-resistant cocksfoot, cv. Cambria, were generally much more aggressive in the competitive environment of the sward than were infected plants of the susceptible cv. S37. Thus, Cambria populations outyielded comparable populations of S37 by only 7% when healthy, but by 40% when infected with CfMV. A few genotypes in the infected Cambria population apparently recovered from the disease, became virus-free and were immune to further infection Resistance to CyMV in C. cristatus takes the form of resistance to systemic spread. A few symptomless and apparently virus-free tillers grow up from the bases of infected tillers. However, these tillers are susceptible to further infection. It is suggested that, in resistant genotypes, CfMV and CyMV trigger the production of antiviral agent(s) which block or restrict virus replication. There is some evidence that CfMV resistance is virus isolate specific, and this could present problems when breeding for durable resistance.     

Progeny tests to identify diploid potato clones homozygous at loci controlling resistance to PLRV

Summary Using potato parental lines homozygous at a locus or loci controlling resistance to potato leafroll virus (PLRV) can give advantages in the selection of resistant forms. In order to identify homozygous diploid clones their test-cross families were evaluated. All the clones that were test-crossed expressed resistance in primarily- and secondarily-infected plants and etiolated sprouts, and were derived from mating genotypes highly resistant to PLRV. Genotypes from test-cross families varied in resistance to PLRV, and one family was found which had only resistant genotypes, suggesting that the resistant parent of this progeny was homozygous at resistance loci. Evidence was gathered that resistance in some diploid clones may result from resistance to virus multiplication as well as restricted virus transport from leaves to tubers.     

Volunteer potatoes as a source of potato leafroll virus and potato virus X

Volunteer potatoes were investigated as infection sources for potato leafroll virus (PLRV) and potato virus X (PVX) in a high elevation seed potato growing area of eastern Idaho. Population densities ofMyzus persicae were assessed. Percentage of PLRV and PVX infection of the volunteers and seed potato crops was determined, as well as density of volunteers and certain parameters of volunteer growth and reproduction. Volunteers apparently harbored no more PLRV than the potato crop from which they originated. But they were found to be an important reservoir of PVX with the infection increasing as much as 12.43% in one year. No aphids capable of transmitting PLRV were found although one species that can transmit potato virus Y was recorded. The mean density of volunteers varied from 0 to 84,880 stems/ha. The number of tubers remaining in the field after harvest and winter weather conditions appeared to be the only factors affecting volunteer density. Volunteer plants arising from seed pieces at an average depth of 6.1 cm were found to set an average of 2.1 new tubers per plant at an average depth of 4.0 cm. These results suggest that volunteer potatoes are a significant source of PVX infection in subsequent seed potato crops.     

野生马铃薯抗PVY材料的鉴定与筛选

通过人工接种的方法对5类野生马铃薯材料进行了马铃薯Y病毒(PVY)的抗性鉴定和筛选。它们对PVY抗性存在明显的差异,其中Solanum stoloniferum(S.A2)×S.stenotomum(104)和S.stoloniferum(S.A5)×S.stenotomum(105)组合抗性最强,属于抗病群体,S.chacoense×S.stenotomum(103)组合属于中抗群体,S.chacoense(102)和S.demissum(101)组合属于感病群体。并从中筛选出一批抗PVY的育种材料:0级抗性材料108份,1级抗病材料56份,3级抗病材料94份。     

Acquisition of potato leafroll virus byMyzus persicae from secondarily-infected potato plants of different genotypes

Summary The acquisition of potato leafroll virus (PLRV) byMyzus persicae nymphs from the top leaves of potato plants was studied throughout a growing season in relation to the antigen titre in those leaves and the feeding behaviour of the aphid. Secondarily-infected plants of eight potato genotypes with different levels of field resistance served as virus sources. Early in the growing season, plants were efficient sources for virus acquisition. The amount of viral antigen detected inM. persicae nymphs fed on the top leaves was strongly correlated with the titres of viral antigen in these leaves. Virus acquisition from the top leaves of older potato plants was markedly impaired and could not be correlated with their virus titre. With increasing age of the potato plants and the development of virus symptoms, the virus titre in the leaves declined and the initial weak correlation between the virus titre and field resistance ratings disappeared. Thus, screening secondarily-infected potato plants for field resistance to PLRV based on the concentration of viral antigen in leaves or in aphids fed on them should be avoided later in the growing season. The feeding rate ofM. persicae, measured by the number of honeydew droplets excreted, did not account for the reduced uptake of virus from older plants since it was not influenced by the age of the plant. Throughout the growing season, the feeding rate ofM. persicae nymphs on PLRV-infected plants was higher on genotypes with low levels of field resistance to PLRV than on genotypes with high ones.     

Production of seed potatoes in Cyprus: the effects of roguing and planting date on the spread of potato leaf roll virus,tuber yield,and infestation by potato tuber moth

Summary Field experiments during 1984–6 tested the effects of planting date on the development of aphid infestations and the spread of potato leaf roll virus (PLRV) in rogued or unrogued plots of potatoes, cv. Spunta. Plantings were made each month from December to April, the customary time for planting being February. Aphid infestation in early-planted plots was severe throughout the growing season; plots planted in February were also severely infested early in the growing season but the populations later gradually declined to undetectable levels. Nevertheless, the incidence of PLRV in the latter plots was as high as in those planted in December-January. Late-planted crops escaped aphid infestation and PLRV infection, either in part (March planting) or completely (April planting). Such crops, however, were uneconomical due to poor yields and heavy losses from potato tuber moth infestation. Roguing significantly reduced the spread of PLRV in all years but its interaction with planting date was insignificant.     

Inheritance of extreme resistance to potato virus Y in potato

Summary Segregation for extreme resistance to PVY was evaluated in progenies derived from crossing two extremely resistant potato clones with parents differing in resistance. Resistance was evaluated after mechanical inoculation with PVY^O and PVY^N, and after graft inoculation with PVY^O. Biological and serological tests (ELISA) were used for virus detection. The extreme resistance is governed by a single dominant gene, but observed segregations deviated from the expected ratios. Considerable modifying effects were detectable, depending on the potato genotype and virus isolate, for a significant excess of susceptible genotypes was observed in some progenies. Moreover, genotypes with non-parental types of resistance to PVY were observed.     

Potato Cultivar and Seed Type Affect the Development of Systemic <Emphasis Type="Italic">Potato virus Y</Emphasis> (PVY<Superscript>N-Wi</Superscript>) Infection

Potato virus Y (PVY) infection is one of the greatest challenges to seed potato production in the United States. To determine how cultivar and seed type affect the development of systemic PVY infection, Russet Burbank and Russet Norkotah Colorado 3 cultivars were grown from two types of pre-nuclear seed (i.e., plantlets and minitubers) and Generation 3 (G3) tubers and challenged with PVY strain Wilga (PVY^N-Wi). Systemic PVY infection was measured by assaying spread of virus from the inoculation site to upper non-inoculated leaves. The Burbank cultivar had a lower incidence of systemic PVY infection compared to the incidence of systemic PVY that developed in the Colorado 3 cultivar. Furthermore, Burbank plants grown from G3 tubers had a lower incidence of systemic PVY infection, as compared to Burbank plants grown from plantlets. Together our results indicate that both cultivar and seed type affect the development of systemic PVY^N-Wi infections post-inoculation.     

Assessing resistance types and levels to epidemic diseases from the analysis of disease progress curves: Principles and application to potato late blight

Both race-specific (RS) and race-non-specific (RNS) resistances exist in potato against the late blight pathogenPhytophthora infestans. Because these resistance types do not have the same epidemiological effects, their presence, alone or combined, in potato genotypes can be deduced from the analysis of disease progress curves from field experiments, a type of data commonly available to potato breeders, and their comparison with those of standard reference cultivars. The identification of RS resistance is based on the presence of a delay in epidemic onset compared to a susceptible cultivar, whereas the identification of RNS resistance is translated into a reduction of apparent infection rates. These parameters can be easily computed after linearization of the disease progress curves. This paper assesses the reliability of this identification using sets of experimental data, discusses its limitations, and highlights potential applications for breeding and cultivar assessment purposes.     

The relation between aphid flights and the spread of potato virus YN (PVYN) in the Netherlands

Summary In the Netherlands early haulm destruction is the main method of preventing excessive spread of virus diseases in seed potato crops. When potato leaf roll virus was the principal problem, the time of haulm destruction was determined by the flight ofMyzus persicae (Sulzer), studied with the aid of Moericke yellow water traps. As potato virus Y^N became a problem and other aphid species interfered, the interpretation of aphid flights became difficult. An attempt is made to quantify total aphid flight activity in terms of risk to the crop. By attributing relative efficiency factors to 9 vector species and considering their flights as recorded with suction traps, values of vector-pressure are obtained that correlate well with weekly infection of bait plants. If accumulated vector pressures are compared with the flights ofM. persicae as recorded with Moericke traps during 1970–1979, it appears that during 6 of these years the critical periods of both systems coincide. However, in 1974 and 1976 much potential vector threat occurred before the start of the flight ofM. persicae. Suggestions are made as to how to apply the method in practice. A semi-popular account of this paper has appeared in Dutch inGewasbescherming 12 (1981) 57–71.     

Genetic Resistances to Potato Leafroll Virus, Potato Virus Y, and Green Peach Aphid in Progeny ofSolanum etuberosum

Increasing prevalence of potato leafroll virus (PLRV) and potato virus Y (PVY) has been reported in seed and commercial potato production, resulting in the rejection of potatoes for certification and processing. Host plant resistance to PLRV and PVY and their primary vector, green peach aphid,Myzus persicae, could limit the spread of these viruses. Host plant resistance to PLRV, PVY, and green peach aphid has been identified in non-tuber-bearingSolanum etuberosum (PI 245939) and in its backcross 2 (BC₂) progeny. Resistance to green peach aphid involved a reduction in fecundity and adult aphid size. In addition, one BC₂ individual was identified as possessing a genetic factor that was detrimental to nymph survival. PVY resistance was identified in all five BC₂ progenies evaluated in a field screening under intense virus pressure. PLRV resistance was identified in two of the five BC₂ progeny. This resistance was stable in field and cage evaluations with large populations of viruliferous aphids. Based on the segregation of virus resistances in the BC₂ , PVY and PLRV resistances appear to result from the action of independent genetic mechanisms that reduce the levels of primary and secondary virus infection. Two BC₂ individuals, Etb 6-21-3 and Etb 6-21-5 were identified as having multiple resistances to PLRV, PVY, and green peach aphid derived fromS. etuberosum. This germplasm could prove useful to potato breeders in the development of virus-resistant cultivars.     

Screening potatoes for field resistance to early blight

A satisfactory method has been developed for screening potato clones for field resistance to early ligbht. Differences in clonal resistance can be evaluated and have proven to be consistent. Resistance to foliar infection appears to be generally associated with plant maturity. Late maturing selections are generally quite resistant and early maturing selections are usually extremely susceptible. Differences in resistance among named varieties from throughout the world were extremely variable.     

Response of Potato Genotypes to Bacterial Wilt Caused by Ralstonia Solanacearum (Smith)(Yabuuchi et al.) In the Tropical Highlands

Potato varietal resistance to bacterial wilt disease caused by Ralstonia solanacearum Yabuuchi et al., (Microbiology and Immunology 39:897–904, 1995) is the best management of the disease. Because the causal pathogen exhibits strong host-pathogen-environment interaction, screening the potential parents for resistance under the target growing environmental conditions is the first important step for effective resistance breeding. The objective of this study was to determine the response to bacterial wilt of selected potato genotypes currently grown by farmers in Kenya and candidate clones from the International Potato Center to identify parents that can be used in the local breeding program to develop resistant cultivars. A study was carried out at Kabete, Kenya for three consecutive seasons between November 2011 and February 2013. Thirty six potato genotypes were planted on an inoculated field at the Kenya Agricultural Research Institute (KARI), Kabete using alpha lattice experimental design with three replications. Data collected were days from planting to onset of wilting (DTOW), bacterial wilt incidence (BWI), total tuber weight (ton ha^?1) (TTW), total tuber numbers/hectare (TTN), proportion of ware sized tubers (PWTTW), proportion of symptomatic tubers based on weight (PSTTW), proportion of symptomatic tubers based on tuber numbers (PSTTN) and latent infection (LI) of the tubers. Almost, all the potato genotypes evaluated in this study were susceptible to bacerial wilt. Ranking of genotypes based on resistance differed among the three seasons. On average, the three most resistant genotypes were Kenya Karibu, Kenya Sifa and Ingabire. The study identified eight potato genotypes (Meru, Ingabire, Kenya Karibu, Sherekea, Kihoro, Tigoni, Bishop Gitonga and Cangi) to be used as promising parents for subsequent crosses. The chosen genotypes are prolific in pollen production and popularly grown by Kenyan farmers.     

Changes in the distribution of phenylamide-resistant strains ofPhytophthora infestans (Mont.) de Bary in Ireland 1985–89

Summary Phenylamide-based fungicides were reintroduced to the Irish market in 1985. Mixtures of phenylamides and mancozeb were recommended for no more than 3 sprays in potato late blight control programmes with the last systemic spray to be applied not later than 31 July. Samples of potato foliage infected withP. infestans were collected from the main potato growing areas in Ireland over a five-year period starting in 1985. Using the floating disc method, 11% of the crops sampled in 1985 were found to have phenylamide-resistant strains ofP. infestans present. The number of crops with resistant strains increased rapidly up to 1988 when 83% of the crops sampled had resistance. Factors affecting the distribution of resistance and the efficacy of a phenylamide-based spray programme are discussed.     

The infection pressure of potato leafroll virus and potato virus Y in relation to aphid populations in Cyprus

Summary The infection pressure of two viruses, potato leafroll (PLRV) and potato virus Y (PVY), both common in seed potatoes grown in Cyprus, was determined in three experiments in 1982–83. Virus-free bait plants, of potato and four other species, were exposed weekly to field infection during the growing season (March–June), and then returned to an aphid-free glasshouse for symptom expression. Only tobacco plants produced clear symptoms enabling reliable assessment of PVY infection pressure. When assessed with ELISA or by tuber indexing, the potato plants were efficient baits for both viruses whose infection period commenced at emergence (mid March to early April) and ended within 6–7 weeks. The seasonal trend of aphid populations, determined with Moericke traps or 100-leaf counts, correspond to that of virus spread. Correlation and regression analysis of aphid and virus data implicated the alate form ofMyzus persicae as the principal vector of both viruses.     

Impact of transgenic viral resistance on seed potato certification

It was nearly 100 years ago that the decline in quality and yield observed in potato crops was attributed to the accumulation of pathogens in seed tubers saved from one field generation to the next. As a direct result of this realization, seed potato certification programs have been initiated throughout the world. While these programs have different administrative structures and employ various methodologies, they are all dedicated to the primary goal of producing a sufficient volume of high quality seed tubers as free from disease causing organisms as possible. Viral diseases are especially problematic because they have serious economic affects and their physical and biological properties make them difficult to control. Until recently, certification programs have relied on methodologies including the use of virus-free material obtained from tissue culture, strict adherence to practices that minimize the mechanical and insect spread of virus, physical inspection of seed increase plots to identify and minimize the number of virus-infected plants, and the increase of seed materials for only a limited number of years to insure that any infected materials are “flushed out” of the system. Approximately 10 years ago, it was first observed that virus resistance can be obtained by using transformation techniques to insert viral genes into the genome of plants. Subsequently, researchers in private and public laboratories have shown that potato plants produced in this way are highly resistant or immune to several viral diseases. The effect that the deployment of these materials may have on the seed potato certification process is the subject of this paper.     

US-8 and US-11 genotypes ofPhytophthora infestans from potato and tomato respond differently to commercial fungicides

Isolates ofPhytophthora infestans collected in Canada in 1997 from both potatoes and tomatoes, were tested on potato leaf discs for their response to an equal active ingredient concentration (10 μg a.i./mL) of the following commercial fungicides: Acrobat MZ (Dimethomorph and Mancozeb), Ridomil Gold (metalaxyl-m), Dithane (Mancozeb), Curzate (Cymoxanil), Bravo (Chlorothalonil), and Tattoo C (Propamocarb and Chlorothalonil). Relative percent leaf infection values, estimated on fungicide-treated vs fungicide-free leaf discs, were compared among isolates from the US-8 and US-11 genotypes isolated from the two host plants. Based on an equal concentration of each fungicide’s active ingredients, variations in relative percent leaf infection were recorded between US-8 and US-11 genotypes, and between potato and tomato isolates within each genotype. Bravo and Tattoo C used with similar active ingredients concentrations were the most inhibitory to all groups of isolates. Dithane and Ridomil Gold provided uniform low inhibition againstP. infestans when tested on potato leaf discs. The different behavior ofP. infestans isolates from potato vs tomato suggests that management of late blight in these two important crops must take such differences into consideration. In particular, the nature and concentration of the fungicides to be applied must take into account any information available about genotypes present on each crop.     

马铃薯病毒外壳蛋白融合基因转化马铃薯及其抗病性分析

将多种病毒的有效核酸片断拼接成融合基因转入马铃薯可获得多抗马铃薯材料。针对马铃薯生产中分布广泛、危害严重并经常混合感染的马铃薯X病毒(PVX)、马铃薯Y病毒(PVY)、马铃薯卷叶病毒(PLRV)和马铃薯S病毒(PVS),开展了利用基因工程方法获得兼抗4种马铃薯病毒转基因马铃薯材料的研究。试验在前期获得含4种马铃薯病毒外壳蛋白基因片段的质粒pART27-XSYV-rh的基础上,通过根癌农杆菌(Agrobacterium tumefaciens)介导转化马铃薯(Solanum tuberosum)品种‘陇薯3号’,PCR扩增和PCR-Southern杂交证明,4价融合基因已整合到马铃薯基因组中。qRT-PCR分析表明,该融合基因在转基因植株中能正常表达。3株转基因植株的抗病性鉴定结果表明,2株对4种病毒同时具有抗性;1株对PLRV侵染表现阳性,对另外3种病毒同时具有抗性。     

Host genes and transgenes that confer resistance to a Scottish isolate of potato leafroll virus are also effective against a Peruvian isolate

Summary Potato genotypes with host gene-mediated resistance (host-MR) and coat protein-mediated transgenic resistance (CP-MR) to potato leafroll virus (PLRV), were inoculated with a Scottish and a Peruvian isolate of PLRV. The coat protein transgene had been cloned from the Scottish PLRV isolate which had also been used during the screening and selection of genotypes with host resistance. Significantly less PLRV accumulated in plants with either host-MR or CP-MR than in plants of susceptible genotypes or in non-transformed control plants, but the two forms of resistance were equally effective against both PLRV isolates.