Steps toward alternative farming system in rice

An experiment was conducted on rice, in old alluvial soil zone, with bio-fertilizer and different doses of organic manure and chemical fertilizer to develop an alternative farming system which is productive, profitable, enhances soil health and conserves the natural resources over a long term. The experiment was conducted for four consecutive years (2007–2010) and it has been observed that 40% N and 25% P chemical fertilizer can safely be supplemented by low-cost, natural resource based bio-fertilizer (Azotobacter sp. and PSB) at 12 kg ha⁻¹ and organic manure (FYM) at 10.00 t ha⁻¹ to achieve long-term sustainability in rice cultivation that is more productive and profitable.     

Plant-availability to barley of phosphorus in ash from thermally treated animal manure in comparison to other manure based materials and commercial fertilizer

Phosphorus (P) is an essential nutrient and a limited resource, yet excess P is applied to agricultural land and can cause environmental problems in areas with intensive animal farming. In this study, the fertilizing effects of P in several animal manure-based products (including thermal treatment) were tested after application to two agricultural soil types (Jyndevad soil: clay 5.1%, silt 4.1%, sand 88.9%, organic matter 2.1%, total C 1.2% soil dry matter (DM), total P 266 mg kg⁻¹ soil DM, pH 6.3; Rønhave soil: clay 15.4%, silt 32.6%, sand 49.6%, organic matter 2.3%, Total C 1.3% soil DM, total P 488 mg kg⁻¹ soil DM, pH 6.6). The first-year effect of P application was tested in a spring barley crop (Hordeum vulgare L.) and residual P effects were tested in a perennial ryegrass (Lolium perenne L.) crop the following year. Untreated ash from thermally gasified animal manure biogas residue (GA) and a corresponding neutralized acid extract of the ash (ExL) in liquid form were the products in focus. Other products in use were: pelletized pig manure biogas residue (PEL), incinerated PEL (IA), anaerobically digested pig slurry (DS), dried ExL, dried fraction of separated pig slurry (SS), thermally gasified SS (GAs), thermally gasified poultry manure (GAp), crushed triple super phosphate (TSP) and disodium phosphate (DSP) was used as reference P fertilizer. For application of 20 kg P ha⁻¹ mineral P fertilizer replacement value (RV) in the second year in the sandy soil was 76% and 99% for GA, 79% and 123% for IA, 95% and 155% for PEL, 94% and 73% for ExL, 55% and 15% for ExD, 64% and 82% for SS, 104% and 109% for DS, 60% and 95% for GAp, 73% and 111% for GAs, where the first value is based on barley DM yield and the second on barley total P uptake. Tripling the GA application rate to 60 kg P ha⁻¹ in both soils had no significant effect on barley DM yield and P uptake. The overall efficiency for liquid fertilizers was much higher than for solid ones and relative effectiveness (RE) of ExL was comparable to RE of DSP. Despite the low P level in soils, the ryegrass crop grew very well on both soils in the second year, and there was no detectable residual effect of the treatments on grass yield and P uptake. In conclusion, untreated ash and solid manures used in this study were not suitable as starter P fertilizer, but could be used to maintain the level of available P in soil, as there were indications that ash/manure P contributed significantly to plant P uptake during the growing season of barley.     

Exploring options to combine high yields with high nitrogen use efficiencies in irrigated rice in China

In Jiangsu province, Southeast China, high irrigated rice yields (6–8000 kg ha⁻¹) are supported by high nitrogen (N) fertilizer inputs (260–300 kg N ha⁻¹) and low fertilizer N use efficiencies (recoveries of 30–35%). Improvement of fertilizer N use efficiency can increase farmers’ profitability and reduce negative environmental externalities. This paper combines field experimentation with simulation modeling to explore N fertilizer management strategies to realize high yields, while increasing N use efficiency. The rice growth model ORYZA2000 was parameterized and evaluated using data from field experiments carried out in Nanjing, China. ORYZA2000 satisfactorily simulated yield, crop biomass and crop N dynamics, and the model was applied to explore options for different N-fertilizer management regimes, at low and high levels of indigenous soil N supply, using 43 years of historical weather data.On average, yields of around 10–11,000 kg ha⁻¹ were realized (simulated and in field experiments) with fertilizer N rates of around 200 kg ha⁻¹. Higher fertilizer doses did not result in substantially higher yields, except under very favorable weather conditions when yields exceeding 13,000 kg ha⁻¹ were calculated. At fertilizer rates of 150–200 kg ha⁻¹, and at the tested indigenous soil N supplies of 0.6–0.9 kg ha⁻¹ day⁻¹, high fertilizer N recovery (53–56%), partial N productivity (50–70 kg kg⁻¹) and agronomic N use efficiency (20–30 kg kg⁻¹) were obtained with application in three equal splits at transplanting, panicle initiation and booting. Increasing the number of splits to six did not further increase yield or improve any of the N use efficiency parameters.     

Nitrogen use efficiency and residual effect of fertilizers with nitrification inhibitors

Blending fertilizers with nitrification inhibitors (NI) is a technology to reduce nitrogen (N) losses. The application of NI could increase the soil N supply capacity over time and contribute to an enhancement of N use efficiency (NUE) in some cropping systems. The objectives were to determine in a field experiment located in Central Spain (i) the effect of NI-fertilizers applied to maize (Zea mays L.) during two seasons on yield, N content and NUE compared to conventional fertilizers, (ii) the soil residual effect of NI-fertilizers in a non-fertilized sunflower (Helianthus annuus L.) planted during a third season, and (iii) the possible sources of residual N via laboratory determinations. The maize was fertilized with ammonium sulfate nitrate (ASN) and DMPP (3,4-dimethylpyrazole phosphate) blended ASN (ENTEC^®) at two levels (130 and 170 kg N ha⁻¹). A control treatment with no added N fertilizer was included to calculate NUE. The second year, DMPP application allowed a 23% reduction of the fertilizer rate without decreasing crop yield or grain quality. In addition, the sunflower planted after the maize scavenged more N in treatments previously treated with ENTEC^® than with traditional fertilizers, increasing NUE in the cropping systems. After DMPP application, N was conserved in non-ready soil available forms during at least one year and subsequently released to meet the sunflower crop demand. The potential N mineralization obtained from aerobic incubation under controlled conditions of soil samples collected before sunflower sowing was higher for ENTEC^® than ASN or control treatments. A higher δ¹⁵N in the soil indicated larger non-exchangeable NH₄⁺ fixation in soils from the plots treated with ENTEC^® or ASN-170 than from the ASN-130 or the control. These results open the opportunity to increase NUE by designing crop rotations able to profit from the effect of NI on the soil residual N.     

Stable isotope technique in the evaluation of tillage and fertilizer effects on soil carbon and nitrogen sequestration and water use efficiency

Agricultural soil could be made to serve as a sink rather than a source of greenhouse gases by suitable soil management. This study was, therefore, conducted to assess the impact of tillage and fertilizer application on soil and plant carbon and nitrogen fractionation and intrinsic water use efficiency (iWUE). The experiment was a split–split-plot factorial design with three replications. The main plot consisted of two tillage treatments: zero tillage (ZT) and conventional tillage (CT). The sub-plot contained four NPK fertilizer treatments (0, 90, 120 and 150 kg N ha⁻¹), while the sub–sub-plot comprised three poultry manure (PM) treatments (0, 10 and 20 Mg ha⁻¹). Soil carbon and nitrogen sequestration were evaluated using stable isotope of carbon (δ¹³C) and nitrogen (δ¹⁵N). The δ¹³C in maize plant was used to obtain iWUE. It was observed that soil δ¹³C and δ¹⁵N were more depleted under ZT than CT and in plots treated with 20 Mg ha⁻¹ PM (PM₂₀) implying carbon and nitrogen sequestration under ZT and by PM₂₀. Relative to the control, application of PM₂₀ raised soil δ¹⁵N enrichment by 82% and 96% under CT and ZT, respectively. Higher iWUE of 25.7% was obtained under CT and was significantly higher than the iWUE values under ZT in the second year of the study while the iWUE was significantly lower with PM₂₀ application than other fertilizer treatments. The significant δ¹³C depletion and hence lower iWUE with combination of NPK fertilizer and PM under CT than the control implied that soil disturbance under tilled plots was mediated by combined nutrient management thereby limiting soil C available for fractionation resulting in lower iWUE. This suggests that conservation tillage such as zero tillage and integrated application of organic and inorganic fertilizers are good strategies for reducing soil carbon and nitrogen emission.     

Organic amendment and minimum tillage in winter wheat grown in Mediterranean conditions: Effects on yield performance,soil fertility and environmental impact

The experiment was conducted to evaluate the agronomic benefit of the application of organic fertilizers combined with different soil tillage on quantitative and qualitative components of winter wheat (Triticum durum Desf., cv. ‘Simeto’) and on chemical soil fertility parameters. The environmental impact, due to heavy metals introduced in soil-plant system, was further investigated. Soil tillage treatments consisted of conventional (CT) and minimum tillage (MT). Fertilization treatments were: mineral at 100 kg N ha⁻¹ (N_min); municipal solid waste compost at 100 kg N ha⁻¹ (N_comp); 50 kg N ha⁻¹ of both compost and mineral fertilizers (N_mix); sewage sludge at 100 kg N ha⁻¹ (N_ss). These treatments were compared with an unfertilized control (N₀). No significant difference was observed between the two soil tillage treatments for quantitative yield production, while among the fertilization treatments N_ss did not show any significant difference compared to N_min. At the end of the research, the fertility of the soil (oxidable carbon, total nitrogen, available phosphorus) was on average higher in N_comp and N_ss treatments compared to the N₀ and N_min ones. The overall distribution of heavy metals in soil-plant system respect to the different fertilizer treatments has not allowed to grouped their effects with Principal Components Analysis. This result showed that the amount of potential pollutants applied by organic amendments did not modified the dynamic equilibrium of the soil–plant system. The MT, as well as the fertilization with the application of sewage sludge (N_ss), allowed to reach productive performance similar to conventional management (CT with N_min). Here we demonstrate that, in the short term period, sustainable agronomical techniques can replace the conventional one with environmental benefit.     

Fertilizers,hybrids, and the sustainable intensification of maize systems in the rainfed mid-hills of Nepal

In the rainfed mid-hill region of Nepal, most fields receive 2–3 t ha⁻¹ of organic compost application every year. Despite efficient recovery and use of organics in the mixed crop-animal systems that predominant in the mid-hills, depleted soil fertility is widely understood to be a significant constraint to crop productivity, with most farmers achieving maize grain yields below 2 t ha⁻¹. Increased use of fertilizer may arrest and even reverse long-term soil quality degradation, but few farmers in the mid-hills use them at present and existing recommendations are insufficiently responsive to site, varietal, and management factors that influence the productivity and profitability of increased fertilizer use. Moreover, policy makers and development practitioners often hold the perception that returns to fertilizer use in the mid-hills are too low to merit investment. In this study, on-farm experiments were conducted at 16 sites in the Palpa district, Nepal to assess the responsiveness of a maize hybrid (DKC 9081) and an ‘improved’ open-pollinated maize variety (‘OPV’, Manakamana-3) to four nitrogen (N) rates, i.e., 0, 60, 120 and 180 kg ha⁻¹, with each N rate response evaluated at 30:30 and 60:60 kg ha⁻¹ rates of phosphorus (P₂O₅) and potassium (K₂O), respectively. With sound agronomy and high rates of fertilizer (180:60:60 kg N:P₂O₅:K₂O ha⁻¹), grain yields observed in the field experiments exceeded 8 t ha⁻¹ with hybrids and 6 t ha⁻¹ with OPV. Yield levels were lower for OPV than hybrid at every level of applied N, but both genotypes responded linearly to N with partial factor productivity for N (PFP_N) ranging from 14 to 19 for OPV versus 26–30 for hybrid, with improved N efficiencies obtained when P and K rates were significantly higher. Averaged across phosphorus (P) and potassium (K) levels, a $ 1 incremental investment in fertilizer increased the gross margin (GM) by $ 1.70 ha⁻¹ in OPV and by $ 1.83 ha⁻¹ in the hybrid. For the full response of N, requires higher rate of P₂O₅:K₂O and vice-versa and full response to P₂O₅:K₂O does not occur if N is absent. These results suggest that, i) degraded soils in the mid-hills of Nepal respond favorably to macronutrient fertilizers – even at high rates, ii) balanced fertilization is necessary to optimize returns on investments in N but must be weighed against additional costs, iii) OPVs benefit from investments in fertilizer, albeit at a PFP_N that is 36–47% lower than for hybrids, and, consequently iv) hybrids are an effective mechanism for achieving a higher return on fertilizer investments, even when modest rates are applied. To extend these findings across years and sites in the mid-hills, crop growth simulations using the CERES-maize model (DSSAT) were conducted for 11 districts with historical weather and representative soils data. Average simulated (hybrid) maize yields with high fertilizer rate (180:60:60 kg N:P₂O₅:K₂O ha⁻¹) ranged from 3.9 t ha⁻¹ to 7.5 t ha⁻¹ across districts, indicating a high disparity in attainable yield potential. By using these values to estimate district-specific attainable yield targets, recommended N fertilizer rates vary between 65 and 208 kg N ha⁻¹, highlighting the importance of developing domain-specific recommendations. Simulations also suggest the potential utility of using weather forecasts in tandem with site and planting date information to adjust fertilizer recommendations on a seasonal basis.     

Use of biofertilizers obtained from sewage sludges on maize yield

Maize production plays an essential role in global food security. In order to maintain both high quality and maize production, there is a great demand for fertilizers. The main objective of this work was to study, over two experimental seasons, the effect of a biofertilizer obtained from sewage sludge (SS) on the yield and on the quality of maize crops (Zea mays L.). The biofertilizer was applied in two ways: (i) to soil, at rates of 0, 10 and 20 Mg ha⁻¹ before sowing, and (ii) via foliar fertilization, applying 0, 3.6 and 7.2 l ha⁻¹ three times during each growing season accounting for a total rate of 0, 10.8 l ha⁻¹ and 21.6 l ha⁻¹. This study is novel because there are no previous studies of the effect of this biofertilizer on any agricultural crops. The results obtained show that, when the SS was applied directly to the soil, the macro- and micronutrients analyzed in both soil and leaves showed no significant differences between either of the fertilizer treatments. Foliar application of SS, however, increased the leaf concentrations of macro- and micronutrients. When the SS rate was 7.2 l ha⁻¹, grain protein concentration increased significantly by about 30% and the yield increased significantly by about 17% compared with the control treatment (SS not applied). These results suggested that, in order to improve agricultural maize yields, quality and nutritional, this SS should be applied as a foliar fertilizer instead of applying it to soil.     

Fourteen years of applying zero and conventional tillage,crop rotation and residue management systems and its effect on physical and chemical soil quality

Soil management systems may negatively affect the quality of the soil. Policymakers and farmers need scientific information to make appropriate land management decisions. Conventional (CT) and zero tillage (ZT) are two common soil management systems. Comparative field studies under controlled conditions are required to determine the impact of these systems on soil quality and yields. The research presented studied plant and soil physical and chemical characteristics as affected by different agricultural management practices, i.e. ZT and CT, cropped with continuous wheat or maize in monoculture (M) or in a yearly rotation (R) of these two crops, either with residue retention (+r) or without residues retention (?r), in an experimental field in the Transvolcanic Belt of Mexico after 14 years. The dominant factors defining soil quality were organic C, total N, moisture, aggregate stability, mechanical resistance, pH and EC. The principal component combining the variables organic C, total N, aggregate stability and moisture content showed the highest correlations with final yield (R = 0.85 for wheat and 0.87 for maize).After 14 years of continuous practice, ZTM + r and ZTR + r had the best soil quality and produced the highest wheat and maize yields of average 2001–2004 (6683 and 7672 kg ha^?1 and 5085 and 5667 kg ha^?1, respectively). Removing the residues, i.e. treatments ZTM ? r with maize (average 2001–2004: 1388 kg ha^?1) and ZTR ? r and CTR ? r with wheat (average 2001–2004: 3949 and 5121 kg ha^?1), gave the lowest yields and less favourable soil physical and chemical characteristics compared to the other practices. It was found that zero tillage with residue retention is a feasible management technology for farmers producing maize and wheat in the agro-ecological zone studied, resulting in a better soil quality and higher yields than with the conventional farmer practice (maize monoculture, conventional tillage and residue removal).     

Effect of depth of fertilizer banded-placement on growth,nutrient uptake and yield of oilseed rape (Brassica napus L.)

A better understanding of crop growth and nutrient uptake responses to the depth of fertilizer banded-placement in the soil is needed if growth and nutrient uptake responses are to be maximized. A two-year field study covering two rape seasons (2010–2011 and 2011–2012) was conducted to examine the effect of banded-placement of N–P–K fertilizer at various depths on growth, nutrient uptake and yield of oilseed rape (Brassica napus L.). The results showed that fertilization at 10 cm and 15 cm soil depth produced greater taproot length and dry weight than fertilization at 0 cm and 5 cm. 0 cm and 5 cm deep fertilization significantly increased the lateral root distribution at 0–5 cm soil depth, while 10 cm and 15 cm deep fertilization induced more lateral root proliferation at 5–15 cm soil depth. At 36 days after sowing (DAS), 5 cm deep fertilization produced better aboveground growth and nutrient uptake than 10 cm and 15 cm deep fertilization. However, reversed results were observed after 36 DAS. 10 cm and 15 cm deep fertilization produced more rapeseed than 0 cm and 5 cm deep fertilization, moreover, the yield difference was more significant in drought season (2010–2011) than in relatively normal season (2011–2012). In summary, these results preliminarily suggest that both 10 cm and 15 cm are relatively proper fertilizer placement depth when the practice of banding fertilizer is used in oilseed rape production. But from the viewpoint of diminishing the production cost, 10 cm deep fertilization should be recommended in actual farming. Because 15 cm deep fertilization may require higher mechanical power input, and thus resulting in higher cost of production.     

Organic fertilizer effects on growth,crop yield,and soil microbial biomass indices in sole and intercropped peas and oats under organic farming conditions

In a field experiment, peas (Pisum sativum L.) and oats (Avena sativa L.) were grown as sole crops and intercrops, fertilized with horse manure and yard-waste compost derived from shrub and garden cuttings at 10 t C ha⁻¹ each. The objectives were to compare the effects of these organic fertilizer and cropping system in organic farming on (a) yield of peas and oats, grown as the sole crop or intercropped, as well as N₂ fixation and photosynthetic rates, (b) the yield of wheat as a succeeding crop, (c) microbial biomass indices in soil and roots, and (d) microbial activity estimated by the CO₂ evolution rate in the field and the amount of organic fertilizers, recovered as particulate organic matter (POM). In general, organic fertilizer application improved nodule dry weight (DW), photosynthetic rates, N₂ fixation, and N accumulation of peas as well as N concentration in oat grain. Averaged across fertilizer treatments, pea/oat intercropping significantly decreased nodule DW, N₂ fixation and photosynthetic rate of peas by 14, 17, and 12%, respectively, and significantly increased the photosynthetic rate of oats by 20%. However, the land equivalent ratio (LER) of intercropped peas and oats exceeded 1.0, indicating a yield advantage over sole cropping. Soil microbial biomass was positively correlated with pea dry matter yields both in sole and intercropped systems. Organic fertilizers increased the contents of microbial biomass C, N, P, and fungal ergosterol in soil and CO₂ production, whereas the cropping system had no effects on these microbial indices. According to the organic fertilizer recovered as POM, 70% (manure) and 64% (compost) of added C were decomposed, but only 39% (manure) and 13% (compost) could be attributed to CO₂–C during a 101-day period. This indicated that horse manure was more readily available to soil microorganisms than compost, leading to increased grain yields of the succeeding winter wheat.     

Effects of ozone treatments on microbial quality and some chemical properties of lettuce,spinach, and parsley

The effects of distilled, ozonated (12 mg L⁻¹) and chlorinated (100 mg L⁻¹) water treatments on inactivation of Escherichia coli and Listeria innocua inoculated on lettuce, spinach, and parsley and on some chemical characteristics (chlorophyll a, chlorophyll b, ascorbic acid, and total phenolic contents and antioxidant activity) of these vegetables were investigated. Chlorine and ozone washes resulted in average log reductions (±standard error) of 2.9 ± 0.1 and 2.0 ± 0.3 for E. coli in the vegetables tested, respectively, while the efficiency of ozone (2.2 ± 0.1 log) was very close to that of chlorine (2.3 ± 0.1 log) on L. innocua. Aqueous ozone did not cause any detrimental effects on the chemical characteristics of the vegetables. The effect of gaseous ozone treatment (950 μL L⁻¹, 20 min) on microbial inactivation and the chemical characteristics of parsley were also determined. This treatment resulted in 1.0–1.5 log reductions in the numbers of both microorganisms but caused significant losses in important bioactive compounds of parsley. Ascorbic acid and total phenolic contents and antioxidant activity in ozone-treated samples were 40.1, 14.4, and 41.0%, respectively, less than the control samples.     

Radiation use efficiency,chemical composition,and methane yield of biogas crops under rainfed and irrigated conditions

For biomethane production, the cup plant (Silphium perfoliatum L.) is considered a promising alternative substrate to silage maize (Zea mays L.) due to its high biomass potential and associated ecological and environmental benefits. It has also been suggested to grow cup plant on less productive soils because of its presumed drought tolerance, but robust information on the impact of water shortage on biomass growth and substrate quality of cup plant is rare. Therefore, this study assesses the effects of soil water availability on the chemical composition and specific methane yield (SMY) of cup plant. Furthermore above-ground dry matter yield (DMY) was analysed as a function of intercepted photosynthetic active radiation (PAR) and radiation use efficiency (RUE). Data were collected in a two-year field experiment under rainfed and irrigated conditions with cup plant, maize, and lucerne-grass (Medicago sativa L., Festuca pratensis Huds., Phleum pratense L.). The cup plant revealed a slight decrease of −6% in the SMY in response to water shortage (less than 50% of plant available water capacity). The average SMY of cup plant [306 l (kg volatile solids (VS))⁻¹] was lower than that of maize [362 l (kg VS)⁻¹] and lucerne-grass [334 l (kg VS)⁻¹]. The mean drought-related reduction of the methane hectare yield (MHY) was significantly greater for cup plant (−40%) than for maize (−17%) and lucerne-grass (−13%). The DMY reduction in rainfed cup plant was mainly attributed to a more severe decrease in RUE (−29%) than for maize (−16%) and lucerne-grass (−12%). Under water stress, the mean cup plant RUE (1.3 g MJ⁻¹) was significantly lower than that of maize (2.9 g MJ⁻¹) and lucerne-grass (1.4 g MJ⁻¹). Compared to RUE, the reduced PAR interception was less meaningful for DMY in rainfed crops. Hence, the cup plant is not suitable for growing on drought prone lands due to its high water demand required to produce reasonably high MHYs.     

Rice dry matter and nitrogen accumulation,soil mineral N around root and N leaching,with increasing application rates of fertilizer

Rice morphology and N leaching, together with mineral N in the soil and soil solution around root, were determined at different growth stages in a 3-year experiment located in the Taihu Lake region, China. The results showed that the N application rates had little impact on the soil mineral N around root, but increased the dry matter and N accumulation aboveground in the high fertility soil (55.3 mg kg⁻¹ of soil mineral N before rice season in 2008). However, no significant difference in grain yield was observed in all N treatments in these 3 years. Path analysis showed that spikelet per panicle made the greatest direct contribution (0.781) and total contribution (0.309) to grain yield compared to other yield components. And a higher panicle per m² and dry matter accumulation resulted in yield decline later in the season due to a decline in the percentage of filled grains.No significant increases in plant N uptake, regardless of N application rates, were observed at the seedling stage, which indicated that lower N application rates could suffice during the rice early growing stages. Nitrate contents, in spite of high N rates input, in the percolation water were all below 1.0 mg L⁻¹ throughout the rice growing season. The increased N rates showed an increment of total N leaching through the percolation water, but not significant. The cumulative total N leaching only accounted for 1.86–4.96% of N fertilizer input, which suggested the N leaching should not be considered as main pollution resources in paddy filed in summer rice season. However, the evaluation of N leaching in different stages indicated that N leaching at seedling stage was larger in dominant (averaged 39.8% of total N leaching) than other stages. For the lower absorbing ability of rice seedling and more N leaching risk, suggestions on N fertilizer reduction should be made at rice early growing stage in this region.     

Agronomic performance of an IR64 introgression line with large leaves derived from New Plant Type rice in aerobic culture

Aerobic culture is a promising water-saving technology in irrigated rice ecosystems, but the vulnerability of plants to fluctuations in soil moisture constrains leaf expansion and yield. The objective of this study was to examine whether an aboveground architecture with large leaves and reduced tillering is associated with vigorous leaf growth in aerobic rice culture. In a series of field experiments, we evaluated the agronomic performance of an IR64 introgression line, YTH323 (IR84640-11-27-1-9-3-2-4-2-2-2-B), with fewer tillers and larger leaves than IR64, derived from New Plant Type rice, under various water and nitrogen conditions. In flooded culture, YTH323 yielded the same as IR64 and 38% more than IR65564-44-51 (a New Plant Type rice) (9.0 vs. 6.6 t ha⁻¹). In aerobic culture, in contrast, it yielded 81% more than IR64 in slightly dry soils (5.1 vs. 2.8 t ha⁻¹). YTH323 had a higher leaf area index than IR64 and IR65564-44-51 under slightly dry soil conditions and under a range of nitrogen conditions. The higher and more stable yield of YTH323 in aerobic culture was attributable to greater early vigor, high specific leaf area, a high ratio of leaf weight to total biomass, and larger leaves, along with the characteristics of high-yield cultivars such as high responsiveness to fertilizers and good grain filling. We conclude that genetic modification of the aboveground architecture of IR64, a typical tropical lowland rice cultivar, to reduce tiller and leaf number improves adaptation to aerobic culture.     

Evaluation of cover-cropping managements on productivity and N-utilization efficiency of kenaf (Hibiscus cannabinus L.), under different nitrogen fertilization rates and soil types

Biomass productivity, nitrogen recovery fraction and nitrogen utilization efficiency (NUE) of kenaf (Hibiscus cannabinus L.) cultivar Tainung 2 were tested, under three Lens culinaries treatments (incorporated, harvested before the sowing of the energy crop and mono-cropping) and four nitrogen dressings (0, 50, 100 and 150 kg ha⁻¹), in two field experiments carried out on a fertile, clayey to loamy soil, and on a sandy soil of moderate fertility, in central Greece, over the period 2007–2009. The obtained results showed a positive response in L. culinaries cover cropping on kenaf total yield, on both experimental sites. Total dry biomass fluctuated from 16.07 to 21.46 t ha⁻¹ for incorporated plots and from 13.63 to 16.55 t ha⁻¹ for control treatments (relied only on applications of N-fertilization) for sandy soil, and from 14.98 to 19.28 t ha⁻¹ in case of legume incorporation and from 12.34 to 16.69 t ha⁻¹ for control plots, for clayey soil, respectively. The evaluated NUE was 76 kg kg⁻¹, for sandy soil, and 72 kg kg⁻¹, for clay soil. The recovery fraction escalated from 41% in control plots to 70% in plots with previous L. culinaries cultivation for sandy soil, while for clayey soil an increase of 20% was recorded, indicating a prominent effect of legume cover-cropping management.     

Rheological and chemical predictors of texture and taste in dessert banana (Musa spp.)

To be able to account for sensory qualities earlier in the assessment of a new banana hybrid in a selection scheme, predicting the sensory perception of banana texture and taste by instrumental parameters was investigated. Thirteen cultivated banana and four new triploid hybrids were characterized by sensory profiling, and rheological and chemical analyses. Multilinear regressions were used to calibrate predictions using 13 cultivated bananas, and the quality of predictions was validated using four hybrids. The sensory characteristics sourness and sweetness were predicted by titratable acidity (R² = 0.68) and pH (R² = 0.66). Malate and citrate were the main contributors to sweetness and sourness. Astringency was predicted by total tannins (R² = 0.55). Rheological parameters from texture profile analyses (stress at fracture, fracturability) were more suitable than pulp puncture force to predict the sensory texture properties firmness (R² = 0.47) and melting (R² = 0.60). These textural properties were predicted by titratable acidity and dry matter content (R² = 0.62). Predictions of mealiness, adhesiveness, and heterogeneity were not efficient. Differences of 3.6–3.7 meq 100 g⁻¹ FW in titratable acidity or of 0.30 g 100 g⁻¹ FW in malate or citrate were required to ensure a detectable difference in sourness or sweetness (p = 0.9). Pulp puncture force needed to differ by a minimum of 0.9 N before a difference in firmness could be perceived by the panelists. In conclusion, while models to predict sourness and sweetness can now be used for high throughput phenotyping, we recommend additional tests for other sensory attributes.     

The effect of plastic mulch on the fate of urea-N in rain-fed maize production in a semiarid environment as assessed by 15N-labeling

A better understanding of the fate of fertilizer nitrogen (N) is critical to design appropriate N management strategies in plastic-mulched croplands. We evaluated the effects of plastic mulch on urea-N recovery by crops and loss from soil in furrow-ridge plots, with and without maize (Zea mays L.) cropping, in a semi-arid rain-fed site in China. We applied the same rate of urea-N (281 kg ha⁻¹) to all treatments during the preparation of the furrow-ridges in 2011 and 2012 but ¹⁵N-labeled the urea in 2011 only. We used transparent film to cover all soil surfaces in the mulched treatments and seeded maize in furrows in treatments with crop. In 2011, plastic mulch increased the total N uptake in the aboveground biomass of maize by 53%, whereas it decreased the in-season labeled-N uptake by 19%, compared to non-mulched treatment. At harvest in 2011, in mulched treatments the total labeled-N remaining in the 0−170 cm soil layer was 25% greater whereas unaccounted labeled-N was 69% less, than in non-mulched treatments, regardless of whether maize was cropped. In 2012 the effect of mulch on total maize N uptake was comparable to that in 2011, but the residual soil labeled-N uptake by maize was 63% higher in mulched compared to non-mulched treatment. At harvest in 2012, plastic mulch increased total labeled-N remaining in the 0−170 cm depth in cropped soils and unaccounted labeled-N in non-cropped soils, compared with no mulch. Our results indicate that plastic mulch profoundly changes the fate of urea-N in maize production in cold and dry croplands.     

The effects of dairy cattle manure and mineral N fertilizer on irrigated maize and soil N and organic C

This work was aimed at providing a sustainable approach in the use of manure in irrigated maize crop under Mediterranean climatic conditions. To this end, the effect of continuous annual applications of dairy cattle manure, combined or not with mineral N fertilizer, on the following parameters was studied: grain yield, grain and plant N concentration, N uptake by plant, N use efficiency, and soil N and organic carbon. The experiment was conducted in a furrow-irrigated sandy soil under dry Mediterranean conditions during seven years. Three different rates of cattle manure (CM): 0, 30 and 60 Mg ha⁻¹, were applied each year before sowing. These CM rates were combined with four mineral N rates (0, 100, 200 and 300 kg N ha⁻¹) applied at sidedress.On average, the highest grain yields during the 7 years were obtained with the combination of CM at 30 Mg ha⁻¹ and mineral fertilizer and with CM at 60 Mg ha⁻¹ without mineral fertilizer. With CM at 30 Mg ha⁻¹, mineral fertilizer increased yields during most of the growing seasons, meanwhile with CM at 60 Mg ha⁻¹, there was not any significant effect of the joint application of mineral fertilizer on yields. Overall, best results were obtained exceeding maximum rates according to present legislation. The mean apparent nitrogen recovery (ANR) fraction during the 7 seasons was 29% for N exclusively applied as CM. Overall, increased N rates applied as CM resulted in decreased ANRs. However, ANR with CM at 30 and 60 Mg ha⁻¹ increased during the first two seasons. This increased ANR ascribed to mineralization of residual organic N applied in previous seasons explained the increasing yields observed in the treatments along the study.The application of CM during 7 years increased the soil organic carbon in the first 30 cm by 5.7 and 9.9 Mg ha⁻¹ with CM at 30 and 60 Mg ha⁻¹, respectively, when compared to the initial stock. Thus, manure-based fertilization could be an alternative to mineral fertilizer in order to achieve high maize yields while improving soil quality under dry Mediterranean conditions.     

Effect of elevated carbon dioxide and temperature on phosphorus uptake in tropical flooded rice (Oryza sativa L.)

A field experiment was carried out to assess the impact of elevated carbon dioxide (CO₂) and temperature on phosphorous (P) nutrition in relation to organic acids exudation, soil microbial biomass P (MBP) and phosphatase activities in tropical flooded rice. Rice (cv. Naveen) was grown under chambered control (CC), elevated CO₂ (EC, 550 μmol mol⁻¹) and elevated CO₂ + elevated temperature (ECT, 550 μmol mol⁻¹ and 2 °C more than CC) in a tropical flooded soil under open top chambers (OTCs) along with unchambered control (UC) for three years. Root exudates were analyzed at different growth stages of rice followed by organic acids determination. Rhizospheric soil was used for analysis of soil phosphatase, MBP and available P. The total organic carbon (TOC) in root exudates was increased by 27.5% and 30.2% under EC and ECT, respectively over CC. Four different types of organic acids viz. acetic acid (AA), tartaric acid (TA), malic acid (MA) and citric acid (CA) were identified and quantified as dominant in root exudates, concentration of these was in the order of TA > MA > AA > CA. The TA, MA, AA and CA content were increased by 34.4, 31.1, 38.7 and 58.3% under ECT compared to that of UC over the period of 3 years. The P uptake in shoot, root and grain under elevated CO₂ increased significantly by 29, 28 and 22%, respectively than CC. Soil MBP, acid and alkaline phosphatase activity was significantly higher under elevated CO₂ by 35.1%, 27 and 36%, respectively, compared to the CC. Significant positive relationship exists among the organic acid exudation, MBP, phosphatase activities and P uptake by rice. The enhanced organic acid in root exudates coupled with higher soil phosphatase activities under elevated CO₂ resulted in increased rate of soil P solubilization leading to higher plant P uptake.