Diversity in Phenotypic Profiles in Landrace Populations of Vietnamese Rice: A Case Study of Agronomic Characters for Conserving Crop Genetic Diversity on Farm

Phenotypic variation of agronomic characters in aromatic rice (Oryza sativa L.) was analyzed in order to elucidate the genetic diversity of the populations of rice landraces. Thirteen populations in Namdinh province, in the Red River Delta of Vietnam, were studied in both of farmers fields and an experimental field. Only small differences were found in agronomic characters among the populations, including populations containing different varieties. Variations among the populations in the frequency distributions of several characters suggested different degrees of diversity among the populations even among populations with the same variety name. The phenotypic profiles of farmers fields were different from those in an experimental field suggesting the environmental differences between them. One variety cultivated in the Myloc district had different phenotypic characters from the other varieties in the Haihau district more clearly in the experimental field. Since the small phenotypic variation in each of farm might be due to the genetic drift and selection by the farmers, on farm conservation of the landraces of rice is considered to be under a force to decrease phenotypic diversity. The present study suggests that the targeting several farms with different phenotypic profiles contribute to the conservation of regional genetic diversity of the landraces of rice.     

Variation of leaf esterases in some Ethiopian tetraploid wheat landraces

Summary Esterase isozymes of tetraploid wheat landraces originating from four localities in the central highlands of Ethiopia were studied using the horizontal starch gel electrophoresis. The landraces studied consisted of 60 Triticum turgidum L., one T. dicoccon Schrank, one T. polonicum L. and an improved local cultivar. One introduced durum cultivar was also included for comparison.A total of 12 esterase zymograms were detected in the landraces. Among these, the types C, A and G were found to be the most prevalent. The esterase isozymes exhibited high variation in the landraces. The esterase zymograms of the introduced durum cultivar and T. dicoccon differed from those of the landraces while the patterns attained in T. polonicum and the improved local cultivar were identical to those of the other landraces.The total number of bands detected was 10, of which 2 were rare, being displayed by only 1 pattern each. The band content of zymograms was in the range of 3–6 and the average was 4. The unique bands were observed in zymograms with both high and low prevalences in the landraces. Two zymograms, including zymogram C which had the highest frequency, occurred in all the localities. The high yielding landraces displayed 4 of the zymograms, one of which belonged to two of the zymograms with widest distribution.     

Natural and human selection for purple-grain tetraploid wheats in the Ethiopian highlands

Summary Purple-grain tetraploid wheats (Triticum turgidum L.) are widely cultivated in the Ethiopian highlands despite the claim that they have lower industrial quality properties and market prices than the white or red/brown seed-colour types. In an attempt to find a possible explanation for this, the three seed-colour groups were compared for grain yield, other 11 agronomic traits and protein content. Five traits displayed significant differences between seed colour groups where the purple-seed was superior; earlier maturity, shorter height, and higher fertility, tillering capacity and harvest index. Most of these are important adaptive traits to waterlogging stress on dark-clay soils (pellic vertisols) where the great bulk of the Ethiopian tetraploid wheats have been grown. Furthermore, among the three seed-colour groups, purple-seed wheat has the best malting quality for the preparation of arekie, a locally distilled spirituous liquor. It, therefore, appears that both natural and human selections have been reponsible for their continued cultivation. Hence, the notion that purple-seeded wheat is the “least preferred” should be interpreted carefully not to necessarily address the whole community in Ethiopia. As to their taxonomy, all tetraploid wheat taxa (T. turgidum L. sensu lato, 2n = 4x = 28) that are found in Ethiopia, with the possible exception of T. dicoccon Shrank (locally known as Adja), may possess the purple pericarp-colour, although in varying frequencies; very low inT. polonicum L., and high inT. carthlicum Nevski andT. durum Desf.     

Genetic Diversity Among Traditional Ethiopian Highland Maize Accessions Assessed by Simple Sequence Repeat (SSR) Markers

Over the past three centuries, maize has become adapted to complex environmental conditions in the highlands of Ethiopia. We analyzed 62 traditional Ethiopian highland maize accessions, using 20 simple sequence repeat (SSR) markers and 15 morphological traits, to assess genetic diversity and relationships among these accessions and to assess the level of correlation between phenotypic and genetic distances. The accessions varied significantly for all of the measured morphological traits. The average number of alleles per locus was 4.9. Pair-wise genetic dissimilarity coefficients ranged from 0.27 to 0.63 with a mean of 0.49. Ward minimum variance cluster analysis showed that accessions collected from the Northern agroecology were distinct from the Western and Southern agroecologies. However, there was no differentiation between the Western and Southern accessions. This suggested gene flow between these regions. The relationship between morphological and SSR-based distances was significant and positive (r = 0.43, p = 0.001). The high genetic diversity observed among these set of accessions, suggests ample opportunity for the development of improved varieties for different agroecologies of Ethiopia. From conservation perspective, sampling many accessions from all agroecologies would be an effective way of capturing genetic variation for future collections and conservation.     

Effect of Developmental Stages Length on Yield and some Quality Traits of Turkish Durum Wheat (<Emphasis Type="Italic">Triticum turgidum</Emphasis> L. Convar. <Emphasis Type="Italic">durum</Emphasis> (Desf.) Mackey) Landraces: Influence of Developmental Stages Length on Yield and Quality of Durum Wheat

The study aimed at exploring durum wheat landraces to be utilized in breeding programs. 566 single durum wheat plants selected from 117 populations collected from 12 provinces were studied. The selected material was planted for characterizing their some qualitative and quantitative traits such as percent vitreousness, pearling index, grain protein content, seed yield and thousand kernel weight; as well as determining time frame for germination-tillering, germination-shooting, germination-heading, germination-maturity, tillering-shooting (T-S), tillering-heading (T-H), tillering-maturity, shooting-heading, shooting-maturity, and heading-maturity. Mean, coefficient of variation, and confidence intervals (0.95) were computed for each of 12 provinces, for altitudinal origins with 200 m of ranges and, for each of two geographical regions separately. The highest variation existed for number of days between T-H and the lowest for number of days between T-S. The highest variation within developmental stages was observed in samples from Diyarbakir with a CV of 32.96%, from 600 to 799 altitude range with a CV of 18.86%, and from Southeast Anatolia with a CV of 20.12%.     

Extent and Pattern of Genetic Diversity for Morpho-agronomic Traits in Ethiopian Highland Pulse Landraces: I. Field Pea (<Emphasis Type="Italic">Pisum sativum</Emphasis> L.)

An experiment was conducted in 2001 at Holetta and Kulumsa, Ethiopia, to study the extent and pattern of genetic diversity in Ethiopian field pea (Pisum sativum L.) landraces. One hundred forty-eight germplasm accessions were grown in an alpha lattice design with 2 replications. Data on 12 traits were collected and analyzed. Differences among the accessions were significant for most of the traits (except number of seeds/ pod) at each location even though differences pooled over location were mostly non-significant. The accessions were grouped into five clusters of different sizes. Accessions from the southern part of the country (Arsi) distributed overall clusters while those from the northern half (North and South Wello, North Gonder and North Shewa) fell into clusters C₁ to C₃. Cumulative effects of a number of characters dictated differentiation of the accessions into clusters. There was no definite relationship between geographic diversity and genetic diversity as overlapping was encountered in clustering pattern among accessions from different parts of the country. Accessions from different regions might have similar genetic background and those from the same origin might also have different genetic background. Therefore, geographic diversity should not necessarily be used as an index of genetic diversity and parental selection should be based on a systematic study of genetic diversity in a specific population. Genetic distances among most of the clusters were significant that crosses between parents selected out of them are expected to generate desirable genetic recombination. Selection should also consider the special advantages of each cluster and each accession within a cluster. Future germplasm collection, conservation and breeding efforts should focus not only on inter-regional diversity but also on intra-regional diversity.     

Farmers’ Perception and Genetic Erosion of Tetraploid Wheats Landraces in Ethiopia

Assessing genetic erosion has been suggested as the first priority in any major effort to arrest loss of genetic diversity. In Ethiopia, although it is generally accepted that significant amount of genetic erosion has occurred and is still occurring, there is little data on its amount and extent. Thus, this study is conducted to quantify the extent of genetic erosion in Ethiopian tetraploid wheat landraces and to identify major causes of genetic erosion. To this end, a field survey of 126 farmers, randomly selected over five districts in eastern, south-eastern and central highlands of Ethiopia during 2001/2002 and 2002/2003 main cropping seasons was undertaken. Questioner was used to collect primary data from farmers who are potentially rich sources of information on genetic erosion at the variety level. Additional data were collected through key informant interviewing. Moreover, resampling was made from Tulo, Chiro and Harar Zuriya districts in eastern Ethiopia. Analysis of history profiles from primary and secondary data indicated a reduction in the use of local varieties over years. Triticum polonicum and T. turgidum are becoming very localized, and therefore, they are under greater threat of extinction. Using the calculation scheme: gene erosion=100%−gene integrity, i.e., the still extant landraces, genetic erosion was calculated for the three different areas where resamplings were made. Genetic erosion of 100% was observed both in T. durum and T. dicoccon in Tulo district. Likewise, genetic erosion of 85.7, 100 and 77.8%, respectively, was calculated for T. durum, T. turgidum and T. dicoccon in Chiro district. In Harar Zuriya, a genetic erosion of 88.9% for T. durum and 100% both in T. turgidum and T. dicoccon was detected. Number of farmers growing landraces of tetraploid wheats drastically decreased in all surveyed areas in the past decades. Displacement of landraces by other crops was the prominent factor for ending landrace cultivation. Farmers’ preference to yield potential and cash crops subsequently reduced the chance of maintaining landraces. Institutional factors like access to credit and the extension advice have influenced farmers’ decision regarding cultivar choice. In all surveyed areas, the most important initial source of seed of improved wheat varieties is the seed credit from the Ministry of Agriculture which uses a ‘plant now, pay later’ scheme to promote the distribution of improved varieties and fertilizers. The problem of genetic erosion through inappropriate maintenance of ex situ collections was also recognized and discussed.     

Genetic Diversity and Relationships of Wheat Landraces from Oman Investigated with SSR Markers

Little is known about genetic diversity and geographic origin of wheat landraces from Oman, an ancient area of wheat cultivation. The objectives of this study were to investigate the genetic relationships and levels of diversity of six wheat landraces collected in Oman with a set of 30 evenly distributed SSR markers. The total gene diversity, (H_T), conserved in the three durum wheat (Triticum durum desf.) landraces (H_T = 0.46) was higher than in the three bread wheat (Triticum aestivum L.) landraces (H_T = 0.37), which were similar to Turkish and Mexican bread wheat landraces calculated in previous studies. Genetic variation partitioning (G_ST) showed that variation was mainly distributed within rather than among the durum (G_ST = 0.30) and bread wheat (G_ST = 0.19) landraces. Based on modified Rogers’ distance (MRD), the durum and bread wheat landraces were distinct from each other except for a few individuals according to principal coordinate analysis (PCoA). One bread wheat landrace (Greda) was separated into two distinct sub-populations. A joint cluster analysis with other landraces of worldwide origin revealed that Omani bread wheat landraces were different from other landraces. However, two landraces from Pakistan were grouped somewhat closer to Omani landraces indicating a possible, previously unknown relationship. Implications of these results for future wheat landrace collection, evaluation and conservation are discussed.     

Extent and Pattern of Genetic Diversity for Morpho-agronomic Traits in Ethiopian Highland Pulse Landraces II. Faba Bean (<Emphasis Type="Italic">Vicia faba</Emphasis> L.)

A field experiment was conducted in 2001 at Holetta and Kulumsa, Ethiopia, to study genetic diversity in Ethiopian faba bean (Vicia faba L.) landraces. One hundred sixty random germplasm accessions were grown in an alpha lattice design with two replications. Data on 12 traits were collected and analyzed. Significant differences were observed among the accessions for most of the traits (except number of pods/podding nodes) at each location even though differences pooled over location were mostly non-significant. Cluster analysis distinguished seven diversity classes of different sizes. Accessions from the northern half of the country (North and South Wello, North Gonder and North Shewa) were closely related while those from the southern part of the country (Arsi) were highly diverse. Cumulative effects of a number of characters dictated differentiation of the accessions into clusters. Some overlapping were encountered between accessions from the northern and those from the southern parts of the country. The study revealed that accessions from different regions might have similar genetic background and those from the same origin might also have different genetic background. Therefore, geographic diversity should not necessarily be used as an index of genetic diversity and parental selection should be based on a systematic study of genetic diversity in a specific population. Genetic distances between most of the clusters were significant that crosses between parents selected out of them are expected to generate desirable progenies. Future germplasm collection, conservation and utilization strategies should put more focus not only on inter-regional diversity in the country as a whole but also on intra-regional diversity in Arsi.     

Diversity, distribution and management of yam landraces (Dioscorea spp.) in Southern Ethiopia

Yam (Dioscorea spp.) is widely grown in many parts of Ethiopia and plays a vital role in local subsistence. Nevertheless, its diversity has not been studied in detail. A survey covering 339 farm households and eight districts was conducted in the major yam growing regions of Southern Ethiopia to investigate the diversity and distribution of yam landraces using structured and semi-structured questionnaires. A total of 37 named landraces were recorded, with a range from one to six (mean 2.9) on individual farms. Farmers’ decisions regarding the number and type of landraces maintained was influenced by tolerance of the landraces to drought, their maturity time and market demand. Most landraces had limited abundance and distribution, and only a few dominant landraces were widely grown. There was also variation amongst districts with respect to diversity, distribution and abundance of the landraces found. In the majority of the localities surveyed, farmers reported a decreasing trend in the number of landraces maintained on individual farms and in the overall yam production. Besides, in those limited areas where yam production is expanding, farmers are increasingly relying on a few selected landraces that mature early. Findings of this study suggest that local farmers in Wolayita and Gamo-Gofa zones maintain considerable yam diversity that remains to be further explored for sustainable utilization and conservation of the available genetic resources.     

Geographic Pattern of Genetic Diversity Among 43 Ethiopian Mustard(Brassica carinata A. Braun) Accessions as Revealed by RAPD Analysis

As an oilseed crop, the cultivation of Ethiopian mustard (Brassica carinata) is restricted only to Ethiopia. Even though geographic diversity is a potent source of allelic diversity, the extent of genetic diversity among germplasm material of Ethiopian mustard from different countries has not been assessed. Forty-three accessions, comprising 29 accessions from eight different geographic regions of Ethiopia and 14 exotic accessions from Australia, Pakistan, Spain, and Zambia were analysed for their genetic diversity using random amplified polymorphic DNA (RAPD) technique. A set of 50 primers yielded a total of 275 polymorphic bands allowing an unequivocal separation of every Ethiopian mustard accession. The usefulness of the 50 RAPD primers in measuring heterozygousity and distinguishing accessions was variable such that polymorphic information content (PIC) varied from 0.05 to 0.40, band informativeness (BI) from 0.05 to 0.65 and primer resolving power (RP) from 0.15 to 6.83. Jaccard's similarity coefficients ranged from 0.44 to 0.87 indicating the presence of a high level of genetic diversity. On the average, Australian and Ethiopian accessions were the most similar while, Spanish and Zambian accessions were the most distant ones. Cluster analysis grouped the 43 accessions into four groups, which has quite a high fit (r = 0.80) to the original similarity matrix. With no prior molecular information, the RAPD technique detected large genetic diversity among the 43 accessions from five different countries and their grouping by dendrogram and principal coordinate analysis (PCoA) was inclined towards geographic differentiation of RAPD markers. Conversely, RAPD differentiation along geographic origin was not apparent within the Ethiopian accessions.     

Dispersal of durum wheat [<Emphasis Type="Italic">Triticum turgidum</Emphasis> L. ssp. <Emphasis Type="Italic">turgidum</Emphasis> convar. <Emphasis Type="Italic">durum</Emphasis> (Desf.) MacKey] landraces across the Mediterranean basin assessed by AFLPs and microsatellites

A comprehensive characterization of crop germplasm is critical to the optimal improvement of the quality and productivity of crops. Genetic relationships and variability were evaluated among 63 durum wheat landraces from the Mediterranean basin using amplified fragment length polymorphisms (AFLPs) and microsatellites markers. The genetic diversity indices found were comparable to those of other crop species, with average polymorphism information content (PIC) values of 0.24 and 0.70 for AFLP and microsatellites, respectively. The mean number of alleles observed for the microsatellites loci was 9.15. Non-metric multi-dimensional scaling clustered the accessions according to their geographical origin with the landraces from the South shore of the Mediterranean Sea closely related. The results support two dispersal patterns of durum wheat in the Mediterranean basin, one through its north side and a second one through its south side.     

Variation in primitive landraces of common bean (<Emphasis Type="Italic">Phaseolus vulgaris</Emphasis> L.) from Argentina

The phenotypic variation found in four common bean (Phaseolus vulgaris L.) complex primitive landraces, among a group of accessions collected in Northwestern Argentina in several missions is described, with particular attention to the wide diversity found in some small areas. It is presented a hypothesis about the maintenance of such diversity in bean mixtures or complex primitive landraces that grow close to their wild relative. Wide diversity regarding to seed type and plant characteristics was displayed by the landraces MCM-SV (composed of 11 lines), MCM-292 (14 lines), MCM-298 (5 lines) and VAV-3716 (14 lines). Food uses of dry seed and fresh pod seemed to be more relevant than the aesthetic use although all of them were presumably considered by humans for centuries resulting in the current phenotypes of these complex primitive landraces. Additionally, some weedy types (intermediate between wild and domesticated types) were detected in the landracesMCM-292 and MCM-298. The four complex landraces described consisted of highly diverse mixtures and they could play a role in breeding to enlarge the genetic basis of domesticated bean varieties belonging to the Andean gene pool.     

RAPD Polymorphism of the White-Flowered Gourd (Lagenaria siceraria (Molina) Standl. Landraces and its Wild Relatives in Kenya

The present study was performed to investigate genetic diversity of Kenyan landraces of the white-flowered gourd (Lagenaria siceraria), which exhibits tremendous morphological variation. RAPD analyses were performed on 53 landraces of the cultivated species L. siceraria and 42 accessions of three wild species (40 L. sphaerica, 1 L. abyssinica, and 1 L. breviflora). A total of 432 polymorphic bands were detected using 54 primers. The four species were clearly differentiated from one another. Intra-specific variations were investigated with L. siceraria and its wild relative L. sphaerica. Landraces of the cultivated species collected from different ethnic communities or regions were differentiated. Morphological variations were not associated with RAPD variations. Bitter landraces collected in Maasai communities showed two specific RAPD bands. In the wild species, accessions collected from the eastern and western sides of the Great Rift Valley were genetically differentiated from each other. In both species, genetic and geographical distance matrices computed among all pairs of accessions were significantly correlated, implying that the observed geographical variation can be explained by the 'Isolation by distance model'. Progeny plants derived from a common mother in L. siceraria showed a low level of segregation in RAPD pattern, suggesting that collected landraces are cultivated, maintaining their inherent traits although they are monoecious and insect-pollinated, whereas the wild relative L. sphaerica showed a higher level of segregation. The morphological diversity observed among landraces of L. siceraria is the result of human selection and their genetic identities are maintained by inbreeding probably resulting from frequent self-pollination.     

Barrier analysis detected genetic discontinuity among Ethiopian barley (Hordeum vulgare L.) landraces due to landscape and human mobility on gene flow

The rate of gene flow is greatly affected by the ability of a species to move from one location to another. In Ethiopia the presence of diverse agro-ecological zones, climatic features, rugged mountains and isolated valleys affected the seed-mediated gene flow among regions. Hence, this study was aimed at investigating and detecting presence of any gene barriers and genetic differentiation among regions. Thus, the study was proposed to test whether the high genetic diversity of barley in Ethiopia was due to a reduced gene flow resulting from geographic barriers and/or effects of human activities. A total of 199 barley landraces collected from 10 different geographic regions and altitudes of Ethiopia were analyzed for 15 molecular markers. A barrier analysis was conducted to identify any geographic areas with pronounced genetic discontinuity between the regions that can be interpreted as barriers to gene flow. The result obtained from analysis of molecular variances indicated high genetic variation within regions rather than between regions. Despite high gene flow among regions, we were able to detect genetic discontinuity due to landscape and human mobility for certain barley growing areas. Hence, it was postulated that these barriers have to be considered in genetic resource sampling strategies.     

Genetic Diversity of Glutenin Protein Subunits Composition in Durum Wheat Landraces [Triticum turgidum ssp. turgidum Convar. durum (Desf.) MacKey] from the Mediterranean Basin

The genetic diversity of high and low molecular weight glutenin subunits of 63 durum wheat landraces from different geographical regions in the Mediterranean Basin was studied using SDS-PAGE. Great variability in glutenin composition was found, with 42 high and low molecular weight glutenin haplotypes, 20 allele combinations at the HMW-GS loci, and 18 at the LMW-GS. All five possible LMW models were detected in all Mediterranean regions. Rare alleles were found at Glu-B1 locus in high frequencies and a priori related alleles to grain quality were also observed. Global genetic diversity index was relatively high (0.67); it ranged from 0.33 to 0.66. Cluster analysis on the frequency patterns of origins grouped genotypes following a geographical structure. Rogers’ distance coefficient on frequency pattern for each region of origin showed two germplasm pools with distinct quality profiles, where South West Asian landraces were very different from the landraces of other Mediterranean areas. The relationship between different regions of origin is discussed and two possible ways of introduction of wheat in the Iberian Peninsula (N Africa and SE Europe) are hypothesized. The use of Mediterranean durum wheat landraces as source of genetic variability for grain quality improvement is highly recommended.     

Genetic Diversity Among Brazilian Cultivars and Landraces of Tomato Lycopersicon Esculentum Mill. Revealed by RAPD Markers

Random amplified polymorphic DNA markers (RAPD) were used to estimate the variability of 35 tomato accessions (Lycopersicon esculentum Mill.). A total of 257 reproducibly scorable bands were obtained from 20 primers, 78.6% of which were polymorphic. The percentage distribution of RAPD markers shows a bimodal distribution, and the frequency of rare alleles is similar in commercial and landrace accessions. Genetic distances among accessions were calculated and a dendrogram showing the genetic relationships among them was constructed allowing for the separation of four groups. Twenty out of 23 Brazilian landraces fell within one group, whereas commercial cultivars were distributed in the four groups. AMOVA analysis of RAPD data showed that, despite the high within Brazilian landraces and commercial cultivars variation, these two groups are significantly different, indicating that landraces can be a source of variation for breeding programs.     

Quantitave evaluation of Ethiopian landraces of lentil (Lens culinaris)

Summary One hundred and fifty-six landrace populations of lentil (Lens culinaris Medikus) collected from 10 provinces in Ethiopia were evaluated for a set of six quantitative traits at three sites contrasting in altitude. Consistent regional differences among landraces were found for time to flower and maturity, 100-seed weight, number of seeds/pod and plant height. The regional differences were clarified by a discriminant analysis based on 100-seed weight, time to flower and plant height. The lentil of the West Highlands was early and short, that of the North Highlands was large-seeded, whereas lentils from the Central Highlands were the least distinctive group. Selection for seed size was the result of local human preferences. Humans were probably also responsible for the lack of adaptive value of plant phenology in relation to altitude. Selection for seed yield at the low and middle elevation sites gave a positive response to selection at both sites. However, selection for yield at the highland site did not give a positive response elsewhere, indicating that adaptation to highland conditions differed from that at lower elevations.     

Exploiting the diversity of barley landraces in Ethiopia

The paper describes a methodology to incorporate the collection of barley landraces available in the Plant Genetic Resources Centre of Ethiopia (PGRC/E), as well as future collections, in the Ethiopian barley breeding program. Six hundred pure lines extracted from thirty Ethiopian barley landraces were evaluated and selected, along with the original populations, between 1989 and 1993. Three lines were identified which significantly outyielded the local landrace in some of the testing sites and had a higher average yield across sites and seasons. The yield of these lines was similar to the best improved variety also developed from a landrace population. Despite the relatively small number of landrace-lines (600) in the original population, the exploitation of the diversity existing within Ethiopian barley germplasm has produced in only five years promising lines for yield at low input conditions and for disease and insect resistance. Some lines had a yield potential comparable with the best breeding lines produced earlier, and one is under multiplication for release.     

Genetic diversity in bambara groundnut (Vigna subterranea (L.) Verdc) landraces assessed by Random Amplified Polymorphic DNA (RAPD) markers

Genetic diversity in 12 landraces of bambara groundnut (Vigna subterranea), an indigenous African legume, was evaluated using Random Amplified Polymorphic DNA (RAPD) markers. DNA from individuals of each landrace was also analysed to determine the level of heterogeneity within landraces. RAPDs revealed high levels of polymorphism among landraces. The percentage polymorphism ranged from 63.2% to 88.2% with an average of 73.1% for the 16 RAPD primers evaluated. The construction of genetic relationships using cluster analysis groups the 12 landraces in two clusters. RAPDs are useful for the genetic diversity studies in V. subterranea and can identify variation within landraces.