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1.
Twenty‐three cytoplasmic male sterile BC1F1 barley lines were exposed to varying temperature treatments (TTs) to examine the effect of temperature on the stability of the expression of cytoplasmic male sterility (cms). The TTs used for this test were: (i) controlled low‐temperature treatment of 16°C (CL), (ii) controlled medium‐temperature treatment of 21–24/16–17°C day/night (CM) and (iii) ambient glasshouse‐temperature treatment of 24–41/16–17°C day/night (AG). The expression of cms was found to be variably influenced by temperature and by the genetic background of the cms recipient lines. Ten cms lines exhibited consistently complete male sterility over TTs, indicating that these lines are completely under cms genes control, while other lines showed partial revision to fertility across different TTs with profound influence of warm temperatures (CM and AG) in breaking down the cms in barley. 相似文献
2.
Temperature related instability of a naturally induced cytoplasmic male sterility in industrial chicory (Cichorium intybus L.)
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Jeroen Van der Veken Stany Vandermoere Olivier Maudoux Christine Notté Tom Eeckhaut Joost Baert Stefaan Werbrouck Johan Van Huylenbroeck 《Plant Breeding》2018,137(3):423-432
Natural alloplasmic cytoplasmic male sterile (CMS) clones of industrial chicory were obtained after crossing wild chicory with selected breeding lines. We investigated the CMS stability of 10 clones in various environmental growing conditions. CMS was stable under cool growing conditions in most of them. Fertility restoration, based on pollen production scores, was observed in all clones after a period of hot temperatures. The early flower bud stage was sensitive, resulting in fertile flowers 12–17 days after exposure to high temperatures. Experiments under controlled growing conditions at 15°C demonstrated that a heat shock of 2 days at 25 or 30°C was sufficient to restore fertility. Sterile flowers were formed when plants were again grown at lower temperatures. Significant differences between individual clones were observed, indicating the potential of genetic selection to obtain stable CMS parent lines. 相似文献
3.
H. Ahokas 《Euphytica》1979,28(2):409-419
Summary 58 varieties, strains or mutants of barley were tested in the msml cytoplasm in Finland, location ca. 61 N. Three (5%) were found to be partial restorers, the rest being maintainers of sterility. 31 Israeli strains of wild barley (ssp. spontaneum) were tested. About one third were maintainers of sterility, the majority partial restorers, and two strains were full restorers. There is probably polymorphism of the restorer gene in Israeli wild barley. Partial restoration displays an environmental response. The physiology of partial restoration is discussed. 相似文献
4.
Summary The use of ethrel for hand crossing in recurrent selection programs with barley is advocated. 相似文献
5.
It is very important for rapeseed hybrid production to develop and utilize a novel cytoplasmic male sterility (CMS) system concerning the possible risk because of a narrow cytoplasm background. Here the anatomy of anther development in the CMS system, named NCa , was observed using light microscope and transmission electron microscope, and the restriction fragment length polymorphism (RFLP) analyses of mitochondrial DNA of NCa sterile line were also performed in comparison with the other rapeseed sterile lines, such as pol , nap , ogura , and tour . The anther abortion of this CMS line occurred at the later uninucleate microspore stage, and the anatomic aborting characteristics were obviously different from all the other rapeseed CMS lines reported before. The RFLP analyses revealed that five probe/enzyme combinations could distinguish the five CMS lines. The results of anatomic observations and mitochondrial DNA polymorphism indicated that the NCa CMS system is a novel one which differs from the pol , nap , ogura , and tour systems. 相似文献
6.
Role of male-sterile cytoplasm in resistance to barley yellow mosaic virus and Fusarium head blight in barley 总被引:2,自引:0,他引:2
Two types of male‐sterile cytoplasm, designated msm1 and msm2, in barley were investigated to determine whether these cytoplasms confer resistance to barley yellow mosaic virus (Ba YMV) and Fusarium head blight (FHB). Alloplasmic lines and isogenic lines of two cultivars showed the same reaction to each Ba YMV as that of their euplasmic lines. This demonstrates that the barley male‐sterile cytoplasms msm1 and msm2 have no effect on resistance to BaYMV. No significant difference in reactions to FHB was recognized among fertile alloplasmic lines of ‘Adorra’, but the difference in reactions to FHB between fertile and sterile isogenic lines of ‘Adorra’ was significant. The damage caused by FHB in the male‐sterile lines that produced sterile pollen was significantly greater than the damage in a sterile line that did not produce pollen. These results suggest that pollen or anthers are important factors in infection with or spread of FHB. For production of hybrid seeds, male‐sterile lines with no pollen production, such as those with msm1 male‐sterile cytoplasm, would reduce FHB infestation. 相似文献
7.
Anther culture was applied as a method to develop the essential components of a cytoplasmic male sterility hybrid system in
rye (Secale cereale L.). These components are the male sterile seed parent (A line), its isogenic maintainer counterpart (B
line) and the restorer pollen parent (R line). Australian rye cultivars were crossed reciprocally to the cultivar ‘Luchs’
which carries the Pampa male sterile cytoplasm (cms-P). Anthers of the F1s in the cms-P cytoplasm (primary cross) and their
reciprocals in the normal cytoplasm (reciprocal cross) were cultured in a modified C17 medium. Male sterile and male fertile
doubled haploids were obtained from the anther culture of the F1s in the cms-P cytoplasm. Testcrosses indicated that the male
sterile doubled haploids were A lines and the male fertile doubled haploids were R lines (restorers). The anther culture of
genotypes in the normal cytoplasm (reciprocal cross) gave all male fertile doubled haploids. Testcrosses indicated that the
male fertile doubled haploids were R lines (restorers) in the normal cytoplasm. The expected maintainer B lines were not identified
because of the limited number of doubled haploids obtained from the anther culture of reciprocal crosses. Experimental single
cross hybrids between male sterile and restorer male fertile doubled haploids showed high levels of heterosis. The results
of this study have significant economic implications especially in the production of hybrids in several species.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
8.
In this study, we report the mapping of the Rf locus in soybean by microsatellite simple sequence repeat (SSR) genetic markers. A cross was made between cytoplasmic male sterility (CMS) line JLCMS82A and restorer line JIHUI 1 based on the DNA polymorphisms revealed by 109 SSR markers. A F2 population derived from a single F1 plant containing 103 individuals was used for mapping the Rf locus. The Rf gene of JIHUI 1 gametophytically restores male fertility to JLCMS82A. Fertile and semi-fertile DNA bulks and parental DNAs were screened with 219 SSR markers, and Satt215 which was previously mapped to soybean LG J, was found linked to the Rf gene. Five additional polymorphic SSR markers from LG J were used for analysis and a regional linkage map around the Rf locus was established. SSR markers, Sctt011 and Satt547, flanked the Rf locus at 3.6 cM and 5.4 cM, respectively. The availability of these SSR markers will facilitate the selection of restorer lines in hybrid soybean breeding. 相似文献
9.
Maternal inheritance of male sterility in the progeny of a natural hybrid between Cajanus lineatus and C. cajan
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Kul Bhushan Saxena Ravikotti Vijaya Kumar Rama Rao Rachit Kumar Saxena 《Plant Breeding》2018,137(2):229-233
Adoption of pigeonpea hybrids in central and southern India is showing high impact with on‐farm yield advantages of >30%. The hybrid pigeonpea technology, the first in any legume crop, is based on a cytoplasmic‐nuclear male‐sterility (CMS) system. For a long‐term sustainability of hybrid programme, it is imperative that both nuclear diversity and cytoplasmic diversity are maintained among hybrid parents. In this context, a continuous search for new CMS‐inducing cytoplasms is necessary. This paper reports detection of maternal inheritance of male sterility in the progeny derived from a natural hybrid between a wild relative [Cajanus lineatus (W. & A.) Maesen comb. nov.] of pigeonpea and an unknown pigeonpea [Cajanus cajan (L.) Millsp.] genotype. In the present study, the male sterility was maintained up to BC7F1 generation by an advanced breeding pigeonpea line ICPL 99044. This male sterility inducing cytoplasm of C. lineatus was tagged as A6. In future, this CMS genetic stock can be used to develop a range of new pigeonpea hybrids with high yield and adaptation. 相似文献
10.
Seed vigour is a precondition for early and homogenous field emergence of barley, in addition to effective malting. This study aimed to assess the selection of barley varieties by using seed vigour as the indicator. Seed vigour of barley (quantified as the germination percentage) was evaluated under drought (?0.2 MPa) and temperature stress (10°C). At two locations over a 3‐year period, 1 population of 133 Derkado × B83‐12/21/5 doubled haploid (DH) lines (and parents) was evaluated for seed vigour, of which 108 DH lines were assessed for three malting parameters. The relatively high values of vigour during the 3‐year period (overall average 94–95%) probably impeded high variations in genetic potential. A total of 27 DH lines of the 133 evaluated showed transgression for vigour (up to 98%) in comparison with the parents (Derkado: 96%; B83: 92%). In conclusion, caution should be applied when selecting for seed vigour, even in good crop years with high levels of seed vigour and low trait variations. Such selection might improve vigour, particularly in crop years with unsuitable weather conditions. 相似文献
11.
Hybrid breeding can exploit heterosis and thus offers opportunities to maximize yield, quality and resistance traits in crop species. Cytoplasmic male sterility (CMS) is a widely applied tool for efficient hybrid seed production. Encoded in the mitochondrial genome, CMS is maternally inherited, and thus, it can be challenging to apply in breeding schemes of allogamous self‐incompatible plant species, such as perennial ryegrass. Starting with a general introduction to the origin and the function of mitochondria in plants, this review focuses on the genetics and biology of CMS systems in plants to identify conserved and system‐specific mechanisms. We examine prospects of comparative mitochondrial genomics to identify candidate genes and causative polymorphisms associated with CMS across species and discuss specificities, obstacles and potentials of CMS as a breeding tool for maximizing heterosis in forage grasses. The purpose of the review is to highlight the importance of CMS and hybrid breeding in grasses, with the aim of facilitating research and development of novel breeding strategies to address the future needs for food, feed and biomass production. 相似文献
12.
Shu‐Yun Chen Szu‐Chien Liu Tzu‐Chuan Lo Andi Wahyono Sz‐Yun Chen Hsin‐Mei Ku 《Plant Breeding》2014,133(2):291-297
Non‐pungent bell pepper (Capsicum annuum L.) lacks the cytoplasmic male sterility (CMS) nuclear restorer allele, Rf, and CMS cannot be employed in its F1 hybrid seed production. To demonstrate that the genic male sterility (GMS) system in non‐pungent bell pepper can be converted to the CMS male sterility system, the conversion of GMS to CMS for non‐pungent bell pepper line GC3 was conducted by introgression of S‐type cytoplasm and the Rf allele from tropical pungent donors. After morphological traits were evaluated, two lines from BC1F1 containing S‐type cytoplasm and four lines from BC2F2 containing Rf allele, phenotypically similar to GC3, were obtained and could be employed as CMS male sterile lines and restorer lines for non‐pungent bell pepper. Four molecular markers potentially linked to traits of interest were also evaluated in BC1F1 and BC1F2 populations. This is the first time that GMS has been successfully converted to CMS in bell pepper, a significant contribution for bell pepper hybrid seed production. 相似文献
13.
14.
Search for male sterility-inducing cytoplasm in wild species of the genus Oryza was attempted with a view to diversify the base of the cytoplasmic genetic male sterility system currently used in the development of commercial rice hybrids. A total of 132 interspecific crosses were made involving accessions of four wild and two cultivated species, all belonging to the A genome. Wild accessions possessing sterility-inducing cytoplasms were identified following reciprocal and sterile F2 backcross methods. Sterile segregants were pursued through substitution backcrosses to develop cytoplasmic male-sterile (CMS) lines. CMS lines were developed with the cytoplasm of either O. rufipogon (VNI) or O. nivara (DRW 21018, DRW 21001, DRW 21039, DRW 21030 and RPW 21111). Based on shape, staining, and abortive pattern of pollen and also on type of interaction with a set of restorers and maintainers for known cytoplasmic male steriles of WA source (V 20A), the newly-developed CMS lines were grouped into four classes. Of these, RPMS 1 and RPMS 2 showed gametophytic male sterility with a restorer reaction different from WA cytoplasmic male-sterile stocks. 相似文献
15.
Summary In the period 1980–1984 about 70000 selfed seeds of the petunia cultivar Blue Bedder were treated with the chemical mutagens ethidium bromide (EB) and ethylnitroso urea (ENU) with the intention to induce (new sources of) cytoplasmic male sterility (CMS). This work resulted in 27 stable male sterile MS-plants, all derived from the ENU-treatments.Crosses with the normal fertile type and with material carrying restorer genes for the common type of CMS in petunia were made. Results obtained from some MS-plants raise a presumption of genic male sterility, probably of the dominant type, having been induced. More detailed investigations are underway. 相似文献
16.
Z. X. Fan W. X. Lei D. F. Hong J. P. He L. L. Wan Z. H. Xu P. W. Liu G. S. Yang 《Plant Breeding》2007,126(3):297-301
Over the past decade, the polima cytoplasmic male sterility ( pol CMS) three-line and two-line systems have been developed for the production of hybrid seed in Brassica napus oilseed rape in China. The discovery of the novel pol CMS restorer line FL-204 is described here. It restores male fertility of hybrid plants in the pol CMS system, but hybrid seed production can only be carried out under autumn sowing in Wuhan in south China under moderate temperatures at flowering. The restorer cannot be used as a male for hybrid seed production in northwestern China (Gansu) under spring sowing conditions, because there it is more or less male sterile due to high temperatures at flowering. Because of this behaviour, it is referred to as a fertility temperature-sensitive restorer (FTSR) in this paper. F2 , BC1 as well as double haploid populations were constructed to determine the inheritance of fertility restoration of FL-204 in the autumn at Wuhan and under spring sowing conditions at Gansu, respectively. Deviations from Mendelian genetics were observed. It was hypothesized that the change of fertility was the result of the interaction between nuclear genes [restoring gene ( Rf ) and temperature-sensitive genes ( ts )] and the cytoplasm. The Rf gene in FL-204 was incapable of restoring male fertility of pol CMS lines under spring sowing conditions at Gansu where it is inactivated by the recessive ts gene present in FL-204. However, the ts gene(s) could be non-functional under moderate temperature conditions at flowering at Wuhan which allows full expression of male fertility in FL-204. The recessive ts gene(s) can only be expressed in plants containing the pol sterile cytoplasm. A method for the utilization of the FTSR pol CMS restorer FL-204 for the production of hybrid seed in B. napus oilseed rape is proposed. 相似文献
17.
Two shallow lowland male sterile lines, MotiA and PadminiAwith Miz.4 cytoplasmic source were developed throughintervarietal hybridization and recurrent backcrossing withtheir respective parents. In all probability, MotiA and PadminiA of Miz.4 source may be genetically different from the Wild abortive CMS source (V.20A). These two CMS lines are semitall in stature, photosensitive, of late duration in flowering with easy fertility restoration ability, and thus could be useful for developing hybrid rice suitable for shallow lowland areas. 相似文献
18.
Six cytoplasmic male sterility (CMS) systems, viz. moricandia, ogura, oxyrrhina, siifolia, tournefortii and trachystoma of Indian mustard (Brassica juncea L.) were characterized for agronomic and floral characteristics. Introgression of alien cytoplasm caused alterations in different floral traits in ogura, siifolia, tournefortii and trachystoma CMS systems. Varied response to different genetic backgrounds of CMS lines indicated the presence of cytoplasmic–nuclear interaction in alteration of floral traits. On the basis of floral characteristics, CMS systems could be grouped into distinct classes. Siifolia, tournefortii and trachystoma CMS lines had narrow petals, while moricandia, ogura and oxyrrhina had wider petals, which were distinguishable on the basis of visual observations. The ratio between length and width of petals were >2.0 in wide petal group but <2.0 in narrow petal group. Further, the relative position of anther and stigma, which was estimated as the ratio between stamen and style length could differentiate the CMS systems. Stamens were longer than styles in oxyrrhina, equal in moricandia and shorter in ogura, siifolia, tournefortii and trachystoma male sterile lines. Non‐viable pollen grains were present in moricandia and oxyrrhina systems, but absent in other systems. In tournefortii and trachystoma, few flowers showed petaloid corolla. All male sterile lines, except trachystoma, which showed crooked siliqua formation were at par with their respective maintainers for flower initiation, plant height, primary branches, seeds per siliqua, seed yield, harvest index, oil and protein content. In general, flower senescence and maturity occurred earlier in male sterile lines than in their respective maintainer lines. 相似文献
19.
Parjeet Singh Aulakh Major S. Dhaliwal Salesh Kumar Jindal Roland Schafleitner Kuldeep Singh 《Plant Breeding》2016,135(4):531-535
Most of the hybrid seed in chilli are produced manually, but the use of male sterility (MS) can reduce the cost of hybrid seed production. MS‐12, a nuclear male‐sterile (NMS) line developed at Punjab Agricultural University, Ludhiana (India), has been utilized to develop commercial F1 hybrids. A recessive gene, designated as ms10, governs MS in MS‐12. Due to recessive gene control, development of new NMS lines incorporating ms10 gene is tedious and time‐consuming. We identified SSR markers AVRDC‐PP12 and AVRDC_MD997* linked to the ms10 gene. A total of 558 primer pairs were screened following bulked segregant analysis (BSA). Linkage analysis in 210 F2 plants indicated that the two SSR markers were linked to the ms10 gene and the marker AVRDC‐PP12 was closest to the gene at 7.2 cM distance. The marker was mapped to chromosome 1 at genome position 175 694 513 to 175 694 644. Until more closely linked markers are developed, the marker AVRDC‐PP12 would facilitate transfer of ms10 gene through marker‐assisted selection (MAS). Fine mapping would lead to cloning of the ms10 gene. 相似文献
20.
The three short duration cytoplasmic genetic male sterility (CGMS) hybrids developed using A2 (Cajanus scarabeoides) cytoplasm-based male sterile lines (CORG 990047A, CORG 990052A and CORG 7A) and the restorer inbred AK 261322 and their segregating populations (F2 and BC1F1) were subjected to the study of inheritance of fertility restoration in pigeonpea. The fertility restoration was studied based on three different criteria, namely, anther colour, pollen grain fertility and pollen grain morphology and staining. The F2 and BC1F1 populations of the three crosses, namely, CORG 990047A × AK 261322, CORG 990052A × AK 261322 and CORG 7A × AK 261322, segregated in the ratio of 3:1 and 1:1, for anther colour (yellow:pale yellow), pollen grain fertility (fertile:sterile) and for pollen grain morphology and staining. The above study confirmed that the trait fertility restoration was controlled by single dominant gene. This finding can be utilized for the identification of potential restorers, which can be further used in the development of CGMS-based hybrids in pigeonpea. 相似文献