Breeding for improvement of flowering attributes of winterhardy rosa kordesii Wulff hybrids

Summary The results from tetraploid hybrids of R. kordesii showed that flowering attributes can be improved by a suitable combination of modifying factors as was shown previously by investigations with diploid hybrids from R. rugosa. A longer flowering period than 7 weeks indicated always the ability for recurrent bloom in these investigations but a shorter flowering period did not always indicate non-recurrent bloom.The offspring from the cross of the recurrent R. kordesii with the non-recurrent seedling G12 segregated into non-recurrent and recurrent types in a ratio of 3:1 for a tetrasomic inheritance, assuming a duplex segregation and complete dominance.The flowering attributes could be improved without loss of a high level of winterhardiness.     

On the transmission of the yellow flower colour from Rosa foetida to recurrent flowering hybrid tea-roses

Summary To transfer the yellow flower colour of R. foetida to Hybrid Tea roses, F₁'s and backcrosses with Hybrid Tea's were produced. In B₁ populations, yellow, recurrent flowering seedlings occurred. Recurrent flowering was controlled by one recessive gene.     

The induction in vitro of adventitious shoots in Rosa

Summary Adventitious shoots were formed on excised leaves, roots and callus of Rosa persica x xanthina and on excised leaves of R. laevigata and R. wichuraiana on culture media that included BAP and NAA as growth regulators. Shoots formed freely on freshly cultured callus of R. persica x xanthina but their production declined in successive cultures and ceased after twelve weeks. Transplantation to soil was improved by rooting plantlets in cellulose plugs in vitro and transferring plantlets to soil while still in the plugs.     

软枣猕猴桃玫瑰饮料的研制

以新鲜软枣猕猴桃和玫瑰花为主要原料制作复合饮料,通过单因素及正交试验筛选饮料的最佳原料及澄清剂配方。结果表明,软枣猕猴桃玫瑰饮料的最佳配方为:30%软枣猕猴桃汁、15%玫瑰花汁、0.015%阿斯巴甜、0.02%柠檬酸;最佳澄清剂配方为:0.15%壳聚糖、0.1%硅藻土、0.05%明胶。在此工艺条件下制得的饮料具有清澈透明的浅黄色,猕猴桃与玫瑰的香气均匀浓郁,酸甜适口,味道协调。     

Inheritance of winterhardiness in roses

Summary Heritability in the broad sense and the distribution of levels of winterkill were analyzed in the offspring from hardy diploid and from hardy, semi-hardy and tender tetraploid roses. The heritabilities for different parental combinations ranged from 51 to 92%.The offspring from crosses of hardy parents were also hardy and showed little variation in hardiness levels. The offspring from crosses of hardy roses with the semi-hardy R. kordesii and the tender Queen Elizabeth survived the winters without coverage but showed a wider variation in hardiness levels.The desirable level of hardiness, an average winterkill of less than 10%, could be achieved through selection in the first or second generation of breeding, depending on the hardiness levels of the parents. The hypothesis is advanced that winterhardiness in roses is controlled by very few or closely linked genetic factors.Contribution No. 557     

Breeding behavior for tuber protein in Solanum tuberosum and Tuberosum-phureja hybrids

Summary True protein content among tubers within a plant of Solanum tuberosum cv. Oneida was found to be negatively correlated with tuber size. A similar study of S. phureja genotype 148-17 revealed no correlation. Tuber protein was determined for 250 genotypes in each of four hybrid potato populations in a factorial mating design with four potato cultivars as stylar parents mated to four groups of pollen parents (4x cultivars, 4x high protein selections, 2x S. phureja, 2x high protein S. phureja). The hybrid population derived from the 4x high protein selections was significantly higher in protein content than the other populations. High estimates of general combining ability for tuber protein content were found for both stylar and pollen parents. Poor photoperiodic adaptation to growing conditions in northern latitudes was a possible explanation for the variable protein phenotypes typical of S. phureja and the lack of transmission of the high protein character in phureja to 4x-2x hybrids. Although protein content was negatively correlated with total yield, high protein segregates with good yield potential were identified in all four populations.Scientific Journal Series Article 11, 616 of the Minnesota Agricultural Experiment Station.     

Shoot production in cut roses with reference to breeding for winter flowering

Summary In 62 clones of cut roses the numbers of flowering and blind shoots were recorded from February 1975 to January 1976. On average about 25 shoots were harvested per plant, of which 27% were blind. No significant correlation was found between the average numbers of flowering and blind shoots. Progenitors for winter flowering roses should be selected among clones that combine a constant total shoot production with a low percentage of blind shoots.     

Linkage relationships of genes for leaf morphology and double flowering in Matthiola incana

Summary The inheritance and linkage relationships of a leaf morphology gene of Matthiola incana were investigated. The allele for sinuate leaf shape, c, was found to be recessive to the allele for normal entire leaf, C. The c allele was tightly linked to the recessive allele for double flowering, s. The recombination frequency between the two loci was close to zero. The mode of inheritance of the C gene was in accordance with the hypothesis that a pollen lethal gene is responsible for the constant 1:1 segregation ratio of double-flowered (= male sterile) to single-flowered (= fertile) plants in most M. incana breeding lines. The sinuate leaf allele seemed to reduce the frequency and delay the flowering of double-flowered plants. The importance of the C gene as a double flowering marker in the cultivation and the breeding of M. incana is discussed.Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel. No. 1128-E, 1993 series.     

Variability and inheritance of flowering time and duration in Actinidia chinensis (kiwifruit)

We investigated the variability and inheritance of flowering in a seedling population of diploid Actinidia chinensis Planch. We found considerable variation in time to reach 50% flowering (D50) and flowering duration (FD). D50 was highly heritable (h ² > 0.85) suggesting that this trait would respond well to selection in this population, whereas FD was greatly affected by the environment (h ² < 0.20). The results also showed that the flowering times of male vines were more sensitive to the environment than those of female vines. However, significant specific combining ability (SCA) effects could be achieved by targeting specific bi-parental combinations for breeding and selection of flowering time. The diversity in flowering traits among males will facilitate the selection of male pollinizers that coincide in flowering time with present or future female cultivars. The possibility of breeding female cultivars with a short flowering period to improve the consistency of fruit maturity at harvest is discussed.     

Variation between genotypes of carnation (Dianthus caryophyllus cultivars and interspecific hybrids) in time of flowering and response to long days. I. Variation in yield distribution

Summary Early flowering carnation cultivars, responding to artificial long days, can assist the grower to plant the onset of flowering and achieve a better distribution of flower production over the year.A comparative trial is described of 20 carnation genotypes (cultivars and backcrosses of interspecific hybrids), representing a wide variation in flowering time and response to long days. The trial was planted in September in a phytotron under a winter light intensity of 15 W/m² for eight hours per day. In one half of the trial, daylength was increased to 24 hours for 25 days in December-January. At the end of February the plants were transferred to a glasshouse where they were observed until all primary shoots had flowered. The main purpose of the experiment was to analyse the genetic factors responsible for the large differences in flowering behaviour between genotypes. Average flowering dates varied from 132 days from pinching in a Dianthus chinensis hybrid to 181 days in the mediterranean cultivar Raggio di Sole. LD response was most pronounced in the earliest flowering Diantini selections and least in the traditional commercial cultivars. In the former the LD effect was evident in all shoots of a plant, but mostly so in the lower (older) shoots; in the traditional cultivars only the higher placed shoots responded to LD. As a result, the proportion of the shoots actually responding to LD varied from 28% in the traditional cultivars to 54% in the Diantini x D. Allwoodii cv Doris group of genotypes.     

The occurrence of 2 n eggs in the dihaploids derived from Rosa hybrida L.

Dihaploid derivatives of 4x Rosa hybrida L. obtained through parthenogenesis induced by irradiated pollen significantly produce 2n eggs,often accompanied by gametes with higher ploidy levels (3x and 4x). The gamete ploidy level distribution depends on the originating genotype. The ploidy level of the male parent affects the maternal /paternal ratio in the endosperm, which can be involved in the counter-selection of triploid genotypes resulting from the crosses made between dihaploid and 4x Rosa hybrida.The observed segregation ratios of flower doubleness in triploid progenies of dihaploids × R. wichuraïanasuggest that the relevant locus could be positioned far from the centromere.     

Early determination of genotypes for apple scab resistance by forced flowering of test cross progenies

Summary Apple selections with different major genes for resistance to apple scab (Venturia inaequalis) derived from Malus floribunda and M. pumila were crossed with each other. The progenies were screened as young seedlings for their reaction to V. inaequalis race 1. A gene for resistance from M. pumila, causing stellate necrotic (SN) lesions, was epistatic to a second gene for resistance from M. floribunda, causing irregular chlorotic (Chl) lesions. Although in most cases SN, Chl and susceptible phenotypes were clearly distinct, occasionally reactions were difficult to characterize or varied from one inoculation to another. Selected seedlings showing resistant or susceptible reactions were forced to flower in 16–20 months in the greenhouse and test crossed with susceptible cultivars. Test cross seedlings were screened for scab reaction. The presence of both genes for resistance in a resistant plant was indicated by presence of both Chl and SN resistant phenotypes in the test cross progeny. Chi-square analysis of four large progenies produced a good fit to the expected ratio. The use of the forced flowering technique to determine scab resistance genotypes in 28 months demonstrated its value in breeding apples with multiple disease resistance.     

Embryo rescue in Rosa hybrida L.

Summary Two crosses between Rosa hybrida L. cultivars, that fail to produce progenies by conventional means, were carried out. In one of them, total hip abscision occured seven weeks after pollination; in the other one, only 2% of the fertilized hips remained on the plants after eleven weeks. Four- and five-week-old fertilized ovules were isolated in the first cross and six-week-old embryos in the second. The ovules were cultured on six media which differed in their mineral salt and sucrose concentrations while the embryos were cultured on only one of these media and eventually cold treated for one month at 4° C before being placed in a culture room set at 23° C. The embryos that had been isolated in ovulo when they were still not visible under a binocular lens developed atypically. The embryos isolated in ovulo when they were heart-shaped and on average 0.27 mm long performed in ovulo germination on some media and/or enlargement on all of them after two weeks; after another two week culture, once isolated from the ovule, all of them germinated. The cultured isolated embryos, that were exposed to cold, enlarged and germinated more rapidly when placed at 23° C than those not exposed to cold. Furthermore, the plantlets resulting from untreated embryo germination were characterized by large cotyledons which were only partially green. These results are discussed in regard to embryogenesis, precocious germination and dormancy.     

Breeding for resistance to yellow disease of hyacinths. II. Influence of flowering time,leaf characters,stomata and chromosome number on the degree of resistance

Summary Analysis of an incomplete diallel design with 14 hyacinth cultivars and 4 wild accessions of Hyacinthus orientalis showed a significant GCA component for resistance to yellow disease. The late flowering hyacinth cultivars King of the Blues and Marconi and the early flowering Hyacinthus orientalis 70129 were found to be the best combiners for yellow disease resistance. Within a cultivar, the degree of earliness itself was not associated with the degree of resistance. Correlation coefficients of leaf characters and degree of resistance showed that hyacinths with short and/or narrow leaves are generally more resistant than those with long and/or broad leaves. Tetraploid cultivars with few, large stomata tend to be susceptible, diploid ones with many small stomata tend to be the more resistant.     

Breeding widely adapted, popular maize hybrids

Summary World trend is to more maize hectarage grown to fewer, more widely-adapted hbrids. My purpose is to document research events leading to the most popular corn hybrids, Pioneer Brand 3780 and 3732. I provide background on maize adaptedness, on the seed maize business, on commercial maize hybrid development process, and on cultural practice change. I provide historical information on the cultivars: Leaming corn, Reid Yellow Dent, and Minn. 13 were widely-adapted open-pollinated varieties. Shift from open-pollinated to hybrid maize caused a shift in emphasis from local to wider adaptation. The first widely-adapted hybrids were Iowa 939 and U.S. 13. DEKALB 404A was the first popular and most popular double-cross hybrid. Pioneer 349 was the second-most popular double-cross hybrid. The first popular single cross hybrids were DEKALB 805 in medium and DEKALB XL45 in early U.S. Corn Belt in the early-1960's. Cultural practices (higher planting density, narrower rows, more nitrogen, better pesticides, and larger equipment allowing more timely operations) changed greatly in the 1960's and 1970's; allowing fewer, more widely-adapted genotypes to prevail. First hybrid 3780, then 3732 became the most popular hybrid; each exceeded one billion (1 × 10⁹) dollars sales. They were the result of wide-area testing, of utilizing higher plant density stress for selecting inbred lines and for screening hybrids, and of modern information management.     

Regeneration through somatic embryogenesis from petal-derived calli of Rosa hybrida L. cv arizona (hybrid tea)

Summary Somatic embryogenesis in callus cultures of petal explants of rose cv arizona is reported here. The calli from petals initiated on dicamba containing medium were friable and gave rise to embryos after several subcultures while those obtained from other explants did not show embryogenesis. Abscisic acid and phloroglucinol were necessary during maturation and plant development, respectively. The individual embryos grew into true-to-type plants.     

Variation in flowering response to pre-vernalisation growth in swedes (Brassica napus ssp. rapifera)

Summary The effect of pre-vernalisation growth (pre-growth) period on time of flowering was examined in four lines of swedes. No effect was found with the line from cv. Marian. The lines from cvs. Ruta Øtofte and Bangholm Wilby showed increases in time to flowering with increased pre-growth period; maximum delay occurred with 4 wk and 6 wk pre-growth, respectively. Most plants from a line of cv. Criffel showed no effects, but a small proportion did show long delays with pre-growth.     

Factors affecting in vitro flowering and fruiting of green pea (Pisum sativum L.)

Multiple shoots were efficiently regenerated from cotyledonary node and shoot tip explants of Pisum sativum within 15 days on MS medium containing B₅ vitamins and supplelmented with 2.0 mgl^-1 6-benzylaminopurine. The elongated shoots produced on the same medium were excised and transferred to MS medium containing half strength ammonium nitrate (8.25 gml^-1) and supplemented with auxins (indole-3-butyric acid or naphthalene acetic acid) either alone or in combinations with gibberellic acid. Rooting and flowering were observed on the 7th and 15th day after their transfer to rooting medium. The flowers self-fertilised in vitro and produced mature pods within 25 days of rooting. These seeds were germinable both in vitro and in vivo. In vitro seeds sown in pots under field conditions developed into flowering plants, and subsequently produced pods with viable seeds. This revised version was published online in July 2006 with corrections to the Cover Date.     

Wide somatic hybrids of Citrus with its related genera and their potential in genetic improvement

Citrus wild relatives are an untapped germplasm reservoir, which possesses many elite resistance traits. Genetic introgression into Citrus by conventional methods has been greatly hindered by polyembryony, pollen / ovule sterility, sexual / graft incompatibility, long juvenility etc. Somatic hybridization via protoplast fusion may make it possible to transfer genes from wild relatives to Citrus. To date, more than sixty sexually compatible or incompatible intergeneric somatic hybrids between Citrus and its various related wild genera have been produced by PEG - orelectrically - induced fusion. These wide somatic hybrids were identified by morphology, cytology, isozyme, RAPD and RFLP analyses. Genetic variation or recombination was also revealed in some of them. Several sexually compatible combinations have flowered and set fruits. The agronomic performance of these wide somatic hybrids is diverse, and the current results are not conclusive. Somatic hybrids are being tested as rootstocks. The prospects of wide somatic hybridization of Citrus with its wild relatives are discussed. This revised version was published online in July 2006 with corrections to the Cover Date.     

月季育种技术研究进展

月季育种技术近年来有了长足的发展并取得了丰硕的成果, 通过对引种筛选育种、 杂交育种、 芽变育种、 诱变育种、 分子育种等主要育种技术近年来国内外研究成果的报道, 总结了各育种技术的特点及应用情况, 并进行了展望, 提出将分子育种技术的先进手段与传统的杂交育种技术相结合, 并且注重国际交流以学习先进技术和经验, 同时深入对月季发育的细胞学及分子生物学机理的研究, 才能更有效地获得新品种的观点, 以期为月季育种工作提供参考, 缩小与国际先进水平的差距。