投喂蚯蚓对中国明对虾生长及生化组成的影响

以不同比例的鲜活赤子爱胜蚓（Eisenia foetida）和人工饲料配合投喂中国明对虾（Fenneropenaeus chinensis）幼虾,测定各种饵料配比对其生长和生化组成的影响。结果表明,在人工饲料中配合投喂1／4以上的赤子爱胜蚓（干重比）可显著提高中国明对虾幼虾的生长速率（P〈0．05）,但对对虾的成活率没有显著影响。随着蚯蚓投喂量的增加,对虾肌肉水解氨基酸的总量以及其中的必需氨基酸、鲜味氨基酸（谷氨酸Glu、天冬门氨酸Asp、甘氨酸Gly、丙氨酸Ala）和促摄食氨基酸（蛋氨酸Met、赖氨酸Lys、甘氨酸Gly）的含量都呈增加的趋势,分析结果表明,投喂蚯蚓对对虾肌肉中促摄食氨基酸含量的影响最大,鲜味氨基酸次之,对必需氨基酸含量的影响最小。氨基酸营养价值的评价结果表明,投喂蚯蚓可提高对虾肌肉蛋白质的营养价值,改善对虾肌肉品质。随着蚯蚓投喂量的逐渐增加,对虾肌肉脂肪中的必需脂肪酸、∑n-3相对含量及其表示脂肪酸营养价值高低的∑n-3／∑n-6的比值都呈降低的趋势,且在蚯蚓投喂量占日投喂量的1／4以上时达差异显著;对蚯蚓肌肉脂肪酸的分析结果表明：蚯蚓脂肪中的二十二碳六烯酸（DHA）相对含量较低,因此,以蚯蚓作为对虾饲料,特别是亲虾的DHA饲料来源时,应配合投喂DHA含量较高的其他饲料。     

饥饿对凡纳滨对虾能量代谢及脂肪酸组分的影响

试验考察了喂食与禁食状态下,凡纳滨对虾(Litopenaeus vannamei)的生长及其肌肉脂肪酸组成的变化。试验虾初始重为0.2627~0.2715g,水体盐度10,温度为27~30℃。喂食采用蛋白饲料(PD)和无蛋白饲料(FPD),蛋白饲料主要以酪蛋白、明胶为蛋白源,蛋白质含量为43.8%。喂食试验为30d,禁食试验为15d。结果表明,经15d的禁食,凡纳滨对虾幼虾日增重率为-1.85%,成活率为53.33%。饥饿状态下,蛋白质和脂肪是凡纳滨对虾的主要能源物质,且以蛋白质为主。饲喂蛋白质饲料组,虾体肌肉脂质中C18∶2n-6、C18∶3n-3、C20∶4n-6和C22∶6n-3(DHA)的含量明显升高(P<0.05)。禁食(ND)状态下,C18∶2n-6、C20∶5n-3和C22∶4n-6得到保留,而C18∶3n-3、C22∶6n-3的含量明显减少(P<0.05),特别是C22∶6n-3消耗后所剩无几。g     

南非斑节对虾和中国明对虾肌肉营养成分分析比较

对同池饲养的南非斑节对虾和中国明对虾肌肉的营养成分进行了分析比较,结果显示,南非斑节对虾肌肉中的粗脂肪含量显著低于中国明对虾(P0.05);水分、粗蛋白质、粗灰分、氨基酸含量二者没有显著差异。南非斑节对虾与中国明对虾肌肉的脂肪酸组成有较大差异,南非斑节对虾肌肉中的饱和脂肪酸含量显著低于中国明对虾(P0.05),单不饱和脂肪酸含量显著高于中国明对虾(P0.05),多不饱和脂肪酸含量二者无显著差异。南非斑节对虾的n-3族多不饱和脂肪酸含量显著高于中国明对虾的(P0.05),而n-6族多不饱和脂肪酸含量显著低于中国明对虾(P0.01),表明南非斑节对虾合成n-6多不饱和脂肪酸的能力较差。     

蝇蛆蛋白对凡纳滨对虾生长、免疫及肌肉成分的影响

在5000m~2、密度为9.0×10~9尾/hm~2的凡纳滨对虾池塘中,投喂基础饲料和20%蝇蛆蛋白+基础饲料饲养体质量(0.18±1.10~)g的凡纳滨对虾,研究了蝇蛆蛋白对生长性能、非特异性免疫及肌肉成分的影响。150d的饲养结果表明,投喂添加蝇蛆蛋白的饲料时,凡纳滨对虾的增长率(310~.51±11.39)%、相对体质量增加率(6383.33±20.54)%和存活率(64.61±3.47)%显著高于对照组的增长率(287.80±15.6)%、相对体质量增加率(5161.11±32.82)%和存活率(52.89±4.22)%(P0.05),而饲料系数显著低于对照组(P0.05)。饲料中添加20%蝇蛆蛋白显著提高了对虾血清超氧化物歧化酶、碱性磷酸酶、酸性磷酸酶、酚氧化物酶和溶菌酶的活性(P0.05)。饲料中添加20%蝇蛆蛋白显著提高了凡纳滨对虾肌肉中粗蛋白含量、必需氨基酸总量、鲜味氨基酸总量以及必需氨基酸占总氨基酸的比值,而其他体成分差异不明显。     

投喂蝇蛆混养中国明对虾和三疣梭子蟹试验

利用2口1.5 hm2的池塘进行投喂蝇蛆混养中国明对虾和三疣梭子蟹试验,1﹟池不投喂蝇蛆,2﹟池配合投喂30%～50%蝇蛆.试验结果表明,2﹟池对虾、梭子蟹产量分别是1﹟池的1.8倍和1.2倍,成活率分别比1﹟池高10.7%和2.7%;纯利润2﹟池比1﹟池多2.8万元;投入产出比,1﹟池为1∶2.73,2﹟池为1∶3.19.2﹟池虾蟹体质量同期增长高于1﹟池,这一变化在养殖中后期更为显著.投喂30%～50%鲜活蝇蛆混养中国明对虾及三疣梭子蟹效益显著.     

海水和淡水养殖凡纳滨对虾肌肉营养成分的比较

测定和分析了海水和淡水养殖的凡纳滨对虾肌肉常规营养成分、氨基酸含量和脂肪酸组成。试验结果表明,海水养殖凡纳滨对虾肌肉中粗蛋白和粗灰分含量显著高于淡水养殖凡纳滨对虾(P0.05),而水分的含量显著低于淡水养殖凡纳滨对虾(P0.05);海水养殖凡纳滨对虾氨基酸总量、必需氨基酸量、鲜味氨基酸总量均显著高于淡水养殖凡纳滨对虾(P0.05);海水和淡水养殖凡纳滨对虾肌肉中均含有丰富的不饱和脂肪酸,总量分别达68.48%和69.80%,但海水虾肌肉中饱和脂肪酸和单不饱和脂肪酸总量显著高于淡水虾(P0.05),而多不饱和脂肪酸总量显著低于淡水虾(P0.01);海水虾肌肉中n-3多不饱和脂肪酸总量亦显著低于淡水虾(P0.05),表明在海水环境中凡纳滨对虾合成n-3多不饱和脂肪酸的能力较差。综合分析表明,海水养殖凡纳滨对虾肌肉的营养价值和风味稍优于淡水养殖凡纳滨对虾。     

中草药对斑节对虾生长、饲料利用和肌肉营养成分的影响

研究了中草药对斑节对虾Penaeusmonodon(初始体重0.30g.尾-1)生长性能、饲料利用以及肌肉营养成分和氨基酸的影响。6种实验饲料(按顺序分别为1,2,3,4,5和6)中中草药的添加量分别为0,0.5,1.0,2.0,4.0和8.0g·kg-1饲料。除了饲料2之外,斑节对虾的成活率、饲料系数和蛋白质效率都显著(P<0.05)优于对照组(饲料1)。投喂饲料3的斑节对虾增重率与对照组相比,虽然统计学上不存在显著差异,但有较大幅度的提高。投喂添加中草药的饲料对斑节对虾肌肉的水分和蛋白质含量无显著影响,但脂肪含量明显下降。结果表明,斑节对虾饲料中添加适量的中草药能够促进生长、显著提高成活率和降低饲料系数,并改变斑节对虾肌肉中脂肪含量和氨基酸组成。     

饲料中n-3 PUFA/n-6 PUFA比值对罗氏沼虾幼虾生长性能和抗氧化能力的影响

为探讨饲料多不饱和脂肪酸n-3 PUFA/n-6 PUFA比值对罗氏沼虾幼虾生长性能、虾体肌肉组成、抗氧化能力、血清生理指标以及消化能力的影响，实验设计了n-3 PUFA/n-6 PUFA比值分别为0.37 (D1)、0.59 (D2)、0.93 (D3)、1.51 (D4)和4.38 (D5)的5种等氮等脂饲料饲喂罗氏沼虾幼虾8周，每组设4重复，每个重复40尾虾 。结果显示，饲料n-3 PUFA/n-6 PUFA比值对罗氏沼虾幼虾存活率 (SR)无显著影响；实验虾终末体重 (FW)、增重率 (WGR)和特定生长率 (SGR)随饲料n-3 PUFA/n-6 PUFA比值增加先升后降，均在D3组最高；且D3组虾有最大的肝胰腺蛋白酶活性及脂肪酸合成酶活性。虾体肌肉粗蛋白质、水分和灰分含量不受饲料n-3 PUFA/n-6 PUFA比值影响，但总脂肪含量在D3组显著高于其他组；各组虾体肌肉的n-3 PUFA/n-6 PUFA比值的变化趋势与饲料的n-3 PUFA/n-6 PUFA比值变化趋势呈正相关。随饲料n-3 PUFA/n-6 PUFA比值增加，实验虾血清和肝胰腺中超氧化物歧化酶 (SOD)活性、总抗氧化能力 (T-AOC)和血清铜蓝蛋白 (CP)含量均呈现先升后降趋势，并在n-3 PUFA/n-6 PUFA比值为0.93~1.51时达到最大，但丙二醛 (MDA)含量持续上升；D1组血清总胆固醇 (T-CHO)和甘油三酯 (TG)含量显著高于其他组；血清谷草转氨酶 (AST)和谷丙转氨酶 (ALT)活性先降后升，且D3组最低。研究表明，饲料适宜的n-3 PUFA/n-6 PUFA可显著提升罗氏沼虾生长性能和抗氧化能力，对增重率和特定生长率进行折线回归，建议罗氏沼虾幼虾饲料中最适n-3 PUFA/n-6 PUFA比值为0.86~0.94。     

大型水生植物饲养斑节对虾幼苗的初试结果

在８０Ｌ的玻璃水槽中用新鲜和腐烂的水生大型植物及促生长用商品颗粒饲料（４０％粗蛋白）饲养斑节对虾幼苗（ＰＬ５０）３０ｄ以上。投喂大型植物的幼虾之生长和成活率显著高于对照组（投喂颗粒饲料）。投喂新鲜禾本茨藻（１００％）的幼虾与投喂腐烂的海滨蔓藻（６５．４％）、新鲜海滨蔓藻（５８．９％）和颗粒饲料（５２．５％）的幼虾相比成活率最高。投喂腐烂禾本茨藻的幼虾成活率最低（３０．６％）但生长最好（头胸甲长（Ｃ     

蟹塘套养与温室养殖的中华鳖雌体营养组成的比较研究

实验研究了中华绒螯蟹(Eriocheir sinensis)池塘套养与温室养殖的中华鳖(Trionyx sinensis)雌体(分别简称"套养鳖"和"温室鳖")的生物学指数,肌肉和裙边中的常规营养成分、氨基酸含量和脂肪酸组成。结果显示:(1)套养鳖裙边指数略高于温室鳖,而套养鳖肝体指数显著低于温室鳖。(2)套养鳖肌肉和裙边中的水分含量显著高于温室鳖,而两种组织中的粗蛋白含量均以温室鳖较高。(3)套养鳖肌肉和裙边中的总必需氨基酸、总非必需氨基酸和总氨基酸含量均显著低于温室鳖;除色氨酸外,温室鳖肌肉中的大部分必需氨基酸分(EAAS)高于套养鳖,而裙边中的所有EAAS均以套养鳖较高。(4)套养鳖肌肉中的C16∶0、C18∶2n6、C18∶3n6、C20∶2n6和C20∶3n3含量显著低于温室鳖,而其C16∶1、C18∶3n3、C20∶3n6和C20∶4n6含量较高;套养鳖裙边中的C18∶3n6、C20∶2n6、C20∶3n6、C20∶4n6含量显著高于温室鳖,而C18∶1n9、C18∶2n6、C18∶3n3、总n-3多不饱和脂肪酸和n-3/n-6多不饱和脂肪酸之比均以温室鳖较高。结果表明,温室鳖肌肉和裙边的干物质含量较高,尤其是蛋白质含量,而套养鳖的氨基酸和脂肪酸组成更优。     

Effect of Various Dietary Lipid Levels on Quantitative Essential Fatty Acid Requirements of Juvenile Pacific White Shrimp Litopenaeus vannamei

A 6-wk feeding trial was conducted to evaluate the effect of various dietary lipid levels on quantitative requirements for essential fatty acids (EFA) by juvenile Pacific white shrimp Litopenaeus vannamei . A factorial experiment with three dietary lipid levels (3, 6, and 9%) and three dietary levels (0.5, 1, and 2%) of a mixture of n-3 highly unsaturated fatty acids (HUFA) was used. An increase in quantitative requirements for EFA with increasing supplementation level of dietary lipid was not demonstrated. Results suggested that this shrimp species appeared to be able to satisfy its n-3 HUFA requirements when they were supplied at 0.5% of diet; this dietary level may be even lowered. Moreover, depressed growth was observed in shrimp fed diets with the HUFA mixture supplemented at 2%. In addition, the present study showed that increasing the dietary lipid level had an effect on lipid composition of shrimp, by increasing lipid deposition in hepatopancreas and muscle tissue, but without a significant effect on growth.     

Essentiality of Dietary n-3 Highly Unsaturated Fatty Acids in Juvenile Japanese Flounder Paralichthys olivaceus

This study was conducted to confirm the essentiality of dietary n-3 highly unsaturated fatty acids (n-3 HUFA) and to investigate the effects of dietary lipid sources on growth performance, liver, and blood chemistry in juvenile Japanese flounder. Three replicate groups of fish (average weighing 3.0 g) were fed experimental diets containing lauric acid ethyl ester, soybean oil, soybean and linseed oils mixture, and squid liver oil as lipid sources for 13 wk. No significant difference was observed in survival among all groups ( P >0.05). Weight gain, feed efficiency and protein efficiency ratio of fish fed the squid liver oil diet containing high n-3 HUFA level were significantly higher than those of fish fed the other diets ( P 0.05). Saturated and monounsaturated fatty acids of liver polar and neutral lipid fractions in fish fed the diet containing lauric acid tended to increase compared to those of the other groups. Fish fed the diets containing soybean and/or linseed oils, which contained high contents of 18:2n-6 and 18:3n-3, respectively, showed the highest contents of 18:2n-6 and 18:3n-3 in both lipid fractions of the liver ( P 0.05). Significantly higher content of n-3 HUFA was observed in both lipid fractions of the liver from fish fed the diet containing squid liver oil than for fish fed the other diets ( P 0.05). Total cholesterol, glucose, and glutamic-oxaloacetic acid transaminase in plasma were significantly affected by dietary lipids ( P 0.05). Histologically, the liver of fish fed the diet containing squid liver oil had a clear distinction between nuclear and cytoplasm membranes; however, cytoplasm of fish fed the diets containing lauric acid and soybean oil was shrunken, and the hepatic cell outline was indistinguishable. It is concluded that the dietary n-3 HUFA is essential for normal growth, and that the dietary lipid sources affect growth performance, liver cell property, and blood chemistry in juvenile Japanese flounder.     

Incorporation of fatty acids from dietary neutral lipid in eye, brain and muscle of postlarval turbot fed diets with different types of phosphatidylcholine

Previous results demonstrated the stimulating effect of soybean phosphatidylcholine (PC) on the utilization of dietary neutral lipid in larval and postlarval fish. The present study further investigated the effect of the degree of saturation of dietary PC on the enhancement of dietary fatty acid incorporation in lipids of turbot. Newly-weaned turbot were fed for 20 days on four isolipidic diets containing the same amount of highly unsaturated fatty acids (HUFA), presented either as neutral lipid, i.e. fish oil ethyl esters, or as polar lipid. Diet FO was a phospholipid-free control diet. Diets HPC, SPC and FPC were supplemented with 3% hydrogenated soybean PC, 3% native soybean PC and 3% marine fish roe PC, respectively.The three PC-supplemented diets resulted in better growth and higher muscle triacylglycerol levels than the PC-free diet FO. The fish fatty acids were determined in 3 lipid classes (neutral lipid, PC, phosphatidylethanolamine) of 3 organs or tissues (eye, brain and muscle). Despite the identical amounts of n-6 and n-3 fatty acids provided by the soybean oil and by the HUFA ethyl esters, the substitution of 3% hydrogenated coconut oil in diet FO by 3% hydrogenated PC in diet HPC caused, averaged over the various tissues and lipid classes, a 7 to 12% higher incorporation of 18:2n-6, 20:4n-6, 20:5n-3 and a 32% higher 22:6n-3 level in turbot lipid. Diet HPC appeared as efficient as diet SPC for enhancing the incorporation of the n-3 HUFA from the ethyl esters. Feeding diet FPC, in which the n-3 HUFA were provided through the marine PC source, resulted in slightly higher levels of these fatty acids in the fish than feeding the ethyl ester HUFA diets, even if supplemented with PC. Present results confirm the positive effect of PC, either hydrogenated or native, on the utilization of fatty acids provided in the diet as neutral lipid. The slightly higher incorporation of HUFA, when esterified on dietary PC instead of neutral lipid, raises the question regarding the form of intestinal absorption of PL in fish.p>     

Requirement of n-3 long chain polyunsaturated fatty acids for European sea bass (Dicentrarchus labrax) juveniles: growth and fatty acid composition

European sea bass juveniles (14.4±0.1 g mean weight) were fed diets containing different levels of fish oil then of n-3 highly unsaturated fatty acids (n-3 HUFA) for 12 weeks. The fish performance as well as fatty acid (FA) composition of neutral and polar lipids from whole body after 7 and 12 weeks feeding were studied. The requirements of juvenile sea bass for n-3 highly unsaturated fatty acids (n-3 HUFA) were studied by feeding fish diets containing six different levels of n-3 HUFA ranging from 0.2% to 1.9% of the diet, with approximately the same DHA/EPA ratio (1.5:1).

The growth rate at the end of the trial showed significant differences. Fish fed low dietary n-3 HUFA (0.2% DM of the diet) showed significantly lower growth than the diet 3 (0.7%), then no further improvement (P>0.05) of growth performance was seen by elevating the n-3 HUFA level in the diet up to 1.9% (diet 6). No difference in feed efficiency, protein efficiency ratio or protein retention was observed among treatments, nor in protein and total lipid content. However, the n-3 HUFA levels in diets highly influenced fish fatty acid composition in neutral lipid, while polar lipid composition was less affected. Comparison of polar lipid content after 7 or 12 weeks indicated that DHA remained stable at the requirement level, while arachidonic acid decreased with time. Results of this experiment suggest that the requirement for growth of n-3 HUFA of juvenile sea bass of 14 g weight is at least 0.7% of the dry diet.     

n-3/n-6 HUFA对大菱鲆幼鱼生长性能、全鱼脂肪酸组成和血清生化指标的影响

为探讨饲料中不同n-3/n-6高不饱和脂肪酸(HUFA)对大菱鲆幼鱼生长性能、全鱼脂肪酸组成和血液生化指标的影响,配制了6种不同n-3/n-6 HUFA(D1:29.54,D2:23.04,D3:18.97,D4:9.06,D5:6.86,D6:3.87)的实验饲料。以大菱鲆幼鱼(12.18±0.01)g为研究对象,在循环水养殖系统中开展了为期8周的养殖实验。实验共分6组,每组3个重复,每个重复35尾鱼。结果显示:饲料中n-3/n-6 HUFA对大菱鲆幼鱼的成活率(SR)无显著影响;增重率(WGR)、特定生长率(SGR)和蛋白质效率(PER)呈先上升后下降趋势且D6组的显著低于其他各组,D2组蛋白质效率显著高于其他各组。全鱼粗蛋白和灰分均呈先上升后下降趋势;D6组肌肉粗蛋白和灰分显著低于其他各组。全鱼ARA含量随着n-3/n-6 HUFA的下降呈上升趋势;全鱼中EPA、DHA、n-3/n-6多不饱和脂肪酸(PUFA)和n-3/n-6 HUFA均随着饲料中n-3/n-6 HUFA的下降呈下降趋势。血清中酸性磷酸酶(ACP)和超氧化物歧化酶(SOD)随着n-3/n-6 HUFA的变化呈上升趋势;溶菌酶(LZM)和总抗氧化能力(T-AOC)呈先上升后下降的趋势,溶菌酶在D2组达到最大值,总抗氧化能力在D3组达到最大值。综上所述,饲料中n-3/n-6 HUFA在适宜范围(18.97~23.04)显著提高了实验鱼的生长性能和非特异性免疫力,改变了鱼体组织的常规成分和脂肪酸组成。     

Requirements for n-3 highly unsaturated fatty acids in feeding juvenile Iranian sturgeon (<Emphasis Type="Italic">Acipenser persicus</Emphasis>) and its effects on growth,carcass quality,and fatty acid composition

A 60-day feeding experiment was carried out on juvenile Iranian sturgeon (Acipenser persicus) to evaluate the effects of different percentages of canola oil and fish oil containing n-3 highly unsaturated fatty acids (n-3 HUFA) on fish growth and fatty acid composition. The requirement for n-3 HUFA of juvenile Iranian sturgeon (48.4 ± 1.98 g) was studied by feeding the fish with various diets containing six different percentage of n-3 HUFA ranging from 1.56 to 17.25 (% of total fatty acids). Neither the weight gain, feed conversion ratio, condition factor, specific growth rate nor the protein efficiency ratio showed any significant differences between the dietary treatments nor in the body composition of juvenile Iranian sturgeon (P > 0.05); also there were no significant difference with respect to the effect of the dietary treatment (P > 0.05) on the blood parameters, for the content of plasma protein, glucose, cholesterol, and triglyceride. The fatty acid composition of the carcass of the Iranian sturgeon fed with the diets containing various levels of n-3 HUFA was reflected by the dietary fatty acid composition. The content of n-3 HUFA in the fish increased with an increase in dietary n-3 HUFA levels. The results indicate that the dietary n-3 HUFA had no effect on the growth of juvenile Iranian sturgeon.     

Effects of dietary phospholipid and highly unsaturated fatty acid on the gonadal development, tissue proximate composition, lipid class and fatty acid composition of precocious Chinese mitten crab, Eriocheir sinensis

Precocious puberty is a major constraint to the economical rearing Eriocheir sinensis farming. Although dietary phospholipid (PL) and highly unsaturated fatty acids (HUFA) reportedly enhanced ovarian development in normal adult E. sinensis , it had opposite effects of reducing precocity in juveniles . This study investigated the effects of dietary PL and HUFA on survival, gonadal development and biochemical composition of precocious E. sinensis . Two diets with PL and HUFA supplementation (diet A) and deficient (diet B) were formulated and fed to precocious E. sinensis . Although no significant differences were found on survival and gonadosomatic index of crabs fed both diets, crabs fed diet B had significantly higher hepatosomatic index and hepatopancrean lipid content than crabs fed diet A. The percentages, 14:0, 18:1n-9, 20:5n-3 and 22:6n-3, in hepatopancreas, muscle and gonads were positively correlated to the diets. Interestingly, the crabs fed diet B accumulated significantly higher 18:2n-6 in gonads and muscle than those fed diet A. Furthermore, substantially higher 20:4n-6 was detected in the gonad and muscle than in the diets. These results suggested that dietary PL and HUFA had limited effects on male gonad biochemical composition. Meanwhile, the precocious E. sinensis may be capable of negating the negative effects of HUFA deficiency by enhanced tissue 18:2n-6 accumulation and converting 18:2n-6 to 20:4n-6.     

饲料脂肪水平对凡纳滨对虾生长、肌肉和肝胰腺脂肪酸组成的影响

以脂肪水平为4.64%、6.56%、8.47%、10.39%和12.31%的5组试验饲料,饲喂凡纳滨对虾[初始质量(9.84±0.14)g].水温为23.4～27.0℃,60 d.饲养结果显示,体质量增加及成活率以8.47%脂肪含量组最佳;雄虾肝胰腺中的C18:3n-3,雄虾肌肉脂质中C20:4 n-6、C20:5n-...     

The importance of dietary HUFA for meagre larvae (Argyrosomus regius; Asso, 1801) and its relation with antioxidant vitamins E and C

Despite the interest of meagre (Argyrosomus regius) as a fast‐growing candidate for Mediterranean aquaculture diversification, there is a lack of information on nutrition along larval development. Importance of highly unsaturated fatty acids (HUFA) and the antioxidant vitamins E and vitamin C has not been investigated yet in this species. Six diets with two levels of HUFA (0.4% and 3% dw), two of vitamin E (1500 and 3000 mg kg^?1) and two of vitamin C (1800 and 3600 mg kg^?1) were fed to 15 dah meagre larvae. Larval growth in total length and dry body weight was significantly lowest in larvae fed diet 0.4/150/180 and showed few lipid droplets in enterocytes and hepatocytes and lower HUFA contents than the initial larvae. Increase in dietary HUFA up to 3%, significantly improved larval growth and lipid absorption and deposition. Besides, among fish fed 3% HUFA, increase in vitamin E and vitamin C significantly improved body weight, as well as total lipid, 22:6n‐3 and n‐3 fatty acids contents in the larvae. Thus, the results showed that 0.4% dietary HUFA is not enough to cover the essential fatty acid requirements of larval meagre and a high HUFA requirement in weaning diets is foreseen for this species. Besides, the results also pointed out the importance of dietary vitamin E and C to protect these essential fatty acids from oxidation, increase their contents in the larvae and promote growth, suggesting high vitamin E and C requirements in meagre larvae (higher than 1500 and 1800 mg kg^?1 for vitamin E and vitamin C respectively).