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为了研究苹果渣用作反刍动物饲料的饲用价值,以3只安装瘤胃瘘管的陕北白绒山羊母羊为试验动物,采用尼龙袋法测定了苹果渣在陕北白绒山羊瘤胃内的干物质(DM)和粗蛋白质(CP)降解率,并用NRC模型分析了苹果渣的瘤胃降解蛋白(RDP)和瘤胃非降解蛋白(RUP)。结果表明,苹果渣在48h的DM降解率为89.16%,CP降解率为82.89%,且二者之间相关性显著(P<0.01);DM和CP有效降解率分别为61.02%和58.44%;RDP含量为58.52%,说明其蛋白质品质较好。因此,由苹果渣降解特性等可以看出,苹果渣含有较高的营养价值,且适口性好,可用作反刍动物饲料。     

In vivo transmission studies of ��Candidatus Mycoplasma turicensis�� in the domestic cat

The natural transmission routes of the three feline haemotropic mycoplasmas – Mycoplasma haemofelis, ‘Candidatus Mycoplasma haemominutum’, and ‘Candidatus Mycoplasma turicensis’ (CMt) – are largely unknown. Since CMt has been detected in the saliva of infected cats using PCR, we hypothesised that direct transmission via social or aggressive contact may occur. The aim of this study was to evaluate this transmission route. CMt-positive saliva and blood samples were obtained from three prednisolone-treated specific pathogen-free (SPF) cats that were infected intraperitoneally with CMt. Five SPF cats were inoculated with CMt-positive saliva or blood subcutaneously to mimic cat bites, and five cats were inoculated orally with blood or oronasally with saliva to mimic social contact. Blood samples were monitored for CMt infection using quantitative real-time PCR and for seroconversion using a novel western blot assay. Neither oronasal nor subcutaneous inoculation with CMt-positive saliva led to CMt infection in the recipient cats, as determined by PCR, independent of prior prednisolone treatment. However, when blood containing the same CMt dose was given subcutaneously, 4 of the 5 cats became PCR-positive, while none of the 5 cats inoculated orally with up to 500 μL of CMt-positive blood became PCR-positive. Subsequently, the latter cats were successfully subcutaneously infected with blood. All 13 CMt-exposed cats seroconverted. In conclusion, CMt transmission by social contact seems less likely than transmission by aggressive interaction. The latter transmission may occur if the recipient cat is exposed to blood from an infected cat.