Effects of salicylic acid on the behavior of Yali pear infected by <Emphasis Type="Italic">Alternaria kikuchiana</Emphasis> Tanaka

To investigate the inductive effect of salicylic acid (SA) on the resistance of Pyrus bretschneider cv Yali to black spot disease (Alternaria kikuchiana Tanaka), the physiological and biochemical characteristics of detached pear leaves at the age of 5 to 10 days were measured after application of SA. The results showed that exogenous SA significantly improved the resistance of Yali pear (Pyrus bretschneider cv Yali) leaves to black spot disease. For the SA treatment at 0.02 mmol·L⁻¹ SA concentration, the disease index was the lowest, and the induced resistance reached up to 63.9%. Furthermore, SA induced local and systemic resistance of Yali pear against the black spot disease. Expression of systemic resistance in leaves was detectable 3 d after SA treatments and lasted for 10 d. POD, PPO, and PAL activities of Yali pear leaves increased by SA treatment. It is suggested that exogenous SA solution as a chemical activator could induce the resistance of Yali pear to black spot disease.     

Salicylic acid induces the accumulation of defense-related enzymes in Whangkeumbae pear and protects from pear black spot

The ability of salicylic acid (SA) to induce disease resistance was studied with Whangkeumbae pears affected by Alternaria kikuchiana Tanaka. SA (0.02, 0.2 and 2 mmol·L⁻¹ active ingredient) protected Whangkeumbae pear leaves from artificial infection when applied before inoculation. When the concentration of SA reached 0.2 mmol·L⁻¹, the disease-infected index was the lowest, and the rate of induced resistance reached 59.0%. The protection of Whangkeumbae pear leaves was associated with the activation of three defense-related enzymes, SOD, POD and PAL. Accumulation of three enzymes was induced locally in treated leaves and systemically. These results suggested that SA could induce systemic resistance in Whangkeumbae pear leaves by increasing defense-related compounds.     

外源水杨酸对野生龙葵幼苗干旱胁迫的缓解效应

利用20%的PEG6000模拟干旱胁迫,同时用不同浓度（0、0.5、1.0、1.5、2.0 mmol·L^-1）的水杨酸(SA)喷施幼苗,研究了不同浓度水杨酸处理对干旱胁迫下野生龙葵幼苗生长的影响。结果表明：干旱胁迫下,龙葵幼苗植株变矮、鲜重降低、根长缩短,叶绿素和可溶性蛋白含量减少,抗氧化酶活性、游离脯氨酸和丙二醛含量增加;经SA处理干旱胁迫下龙葵幼苗株高、根长和鲜重都有明显增加,特别是1.5 mmol·L^-1 SA处理组分别增加20.8%、28.0%和29.7%,1.5 mmol·L^-1 SA处理组龙葵幼苗叶绿素含量提高31.9%、脯氨酸含量提高65.4%,可溶性蛋白含量提高42.8%,超氧化物歧化酶（SOD）、过氧化物酶（POD）与过氧化氢酶（CAT）活性达到最高,与对照相比分别增加了30.2%、87.8%和50.3%;SA处理使丙二醛（MDA）含量降低了37.8%。综合来看,适当浓度的SA可以缓解干旱对龙葵幼苗造成的伤害,并以1.5 mmol·L^-1外源SA缓解效果最好。     

Effects of salicylic acid (SA), ultraviolet radiation (UV-B and UV-C) on trans-resveratrol inducement in the skin of harvested grape berries

Effects of salicylic acid (SA), ultraviolet radiation (UV-B and UV-C) on the trans-resveratrol (Res) inducement of the skin of harvested grape berries were studied with three grape cultivars Takasuma, Tano Red and Carigane. Split plot design tests were adopted to compare the effects of UV-B and UV-C radiation on Res inducement of different cultivars. Results showed that spraying 100 mg·L⁻¹ SA markedly enhanced Res contents in the skins of harvested berries for the three selected cultivars. However, the effect of SA varied with the cultivars, and Res inducement by SA was more effective to Tano Red than Takasuma and Carigane. UV-B or UV-C irradiation significantly increased Res contents in grape skins and UV-C was more effective than UV-B. The effects of UV types and dosages on Res inducement depended upon cultivars. In the range of 0–3.6 kJ·m⁻², the Res contents in the skins of the three grape cultivars were enhanced along with the increase of dosages of UV-B and UV-C. __________ Translated from Journal of Fruit Science, 2007, 24(1): 30–33 [译自: 果树学报]     

Adventitious shoot regeneration from the leaves of some pear varieties (Pyrus spp.) grown in vitro

The pear (Pyrus spp.) is one of the most important temperate fruit crops. A complete protocol for adventitious shoot regeneration was developed from the leaves of four pear varieties grown in vitro: Abbe Fetel, Yali, Packham’s Triumph and Aikansui, and the Chinese rootstock variety Duli. Shoot explants were collected from the field and cultured in vitro in Murashige and Skoog (MS) media supplemented with 1.0 mg·L⁻¹ 6-benzylaminopurine (BA) and 0.1 mg·L⁻¹ indole-3-butyric acid (IBA). After four weeks, leaf explants of all 5 varieties grown in vitro were excised and cultured in MS media supplemented with 0.0 mg·L⁻¹, 0.2mg·L⁻¹, 0.5 mg·L⁻¹, 1.0 mg·L⁻¹ and 2.0 mg·L⁻¹ naphthaleneacetic acid (NAA) and 5.0 mg·L⁻¹ BA or with 1.0 mg·L⁻¹, 2.0 mg·L⁻¹ and 4.0 mg·L⁻¹ thidiazuron (TDZ). The cultures were maintained in darkness for 21 days for shoot induction in the shoot induction medium (IM), then transferred to the shoot expression medium (EM) in 1.0 mg·L⁻¹ TDZ without any auxins and kept in a growth room at (25±2)°C under a 16/8 h light/dark photoperiod regime for 8 weeks. Finally, the shoots were transferred to the MS shoot elongation medium (SEM) supplemented with 0.2 mg·L⁻¹ BA, 0.1 mg·L⁻¹ IBA and 0.2 mg·L⁻¹ gibberellic acid (GA₃). A combination of TDZ and NAA had a significant effect on the number of shoot regenerations in all 5 tested varieties. The maximum mean number of shoots and maximum number of shoots per leaf obtained from Yali variety were 11.8 (P⩽0.001) and 22, followed by Aikansui with 6.6 (P⩽0.001) and 4.6, and Duli with 8 (P⩽0.001) and 12, all arising from the combination of 0.2 mg·L⁻¹ NAA with 1.0 mg·L⁻¹ TDZ. For Packham’s Triumph and Abbe Fetel, the maximum mean number of shoots and maximum number of shoots per leaf were 5.6 (P⩽0.001), 4.8 and 8 (P⩽0.001), and 11, respectively, from the combination of NAA (1.0 mg·L⁻¹) and TDZ (2.0 mg·L⁻¹). Abbe Fetel was the only variety which produced significantly higher adventitious shoots from the two different combinations of 1.0 mg·L⁻¹ NAA and 5.0 mg·L⁻¹ BA (P⩽0.05), and 2.0 mg·L⁻¹ NAA and 5.0 mg·L⁻¹ BA (P⩽0.01). Some of the most prominent problems associated with shoot proliferation and regeneration were also observed and discussed in this paper.     

不同浓度水杨酸诱导桉树抗焦枯病保护酶活性变化

以前期收集的福建省11个桉树主栽种系中的中抗种系巨尾桉(Eucalyptus grandis×E.urophylla)为试验材料,用浓度为0(CK)、1.0、1.5、2.0、2.5和3.0 mmol.L-1的外源植物生长物质水杨酸(SA)对桉树叶片进行喷雾处理,以提高桉树对焦枯病的抗病性。结果表明,SA能有效诱导桉树提高对焦枯病的抗性,不同浓度SA对桉树的抗病性诱导效果不同。随着SA浓度的增加,桉树叶片中过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)、超氧化物歧化酶(SOD)和多酚氧化酶(PPO)的活性都呈现出先上升后下降的变化趋势。其中,当SA浓度为2.0 mmol.L-1时,桉树叶片中POD、PAL、SOD和PPO的活性都最高。     

Effects of regulators on the respiratory enzyme of pear branches during dormant period

The effects of regulators on the respiratory enzymes of Qiyuesu’s pear branches during dormant period were studied. The results showed that enzyme activities of SDH, G-6-PDH and 6-PGDH were increased by 0.02 mmol/L SA and 120 mg/L GA₃. The enzyme activities of phosphohexoisomerase were reduced by 0.02 mmol/L SA and 120 mg/L GA₃. Compared with the control, the PGI activity was reduced by 20.5% and 13.6% using 0.02mmol/L SA and 120mg/L GA₃. Similarly, the SDH activity was increased by 6.1% and 29.2%, respectively. Likewise, the activity of G-6-PDH and 6-PGDH was increased by 93.9% and 24.8%. Changes of respiratory enzymes were consistent with respiratory pathway of regulators, which indicated that SA and GA₃ were helpful to break the dormancy by enhancing activities of SDH, G-6-PDH and 6-PGDH.     

石灰氮和水杨酸对油桃金山早红花芽休眠的影响

以金山早红油桃为试材,在花芽自然休眠进程的不同阶段施用不同浓度的石灰氮和水杨酸后置于温室中进行萌芽培养,并对芽体内活性氧含量、抗氧化酶活性和呼吸速率变化进行了测定.结果表明:①初休眠期和深休眠期施用水杨酸对油桃破眠无效,后期休眠10%水杨酸对促进油桃破眠有一定效果,萌芽较对照提前8 d,最终萌芽率达100%;在整个休眠期石灰氮处理对破眠无效.②在初休眠期和深休眠期,石灰氮和水杨酸处理的花芽H2O2含量、抗氧化酶和呼吸速率的变化趋势相似.③在休眠后期,芽体的H2O2含量很低,但石灰氮处理的有所升高,而水杨酸处理的则呈下降趋势;过氧化氢酶(CAT)活性在较低水平上有所升高后再继续下降,但水杨酸处理的活性比石灰氮处理的上升幅度小;石灰氮和水杨酸处理的过氧化物酶(POD)活性在低水平上升高,幅度大于对照;而超氧化物歧化酶(SOD)活性则在较高水平上缓慢下降,但10%水杨酸处理的在培养后期有所回升;石灰氮处理的呼吸速率变化幅度很小,但10%水杨酸处理的上升幅度较大.     

Adventitious shoot regeneration from the leaves of in vitro grown ‘Zhongli 1’ pear (Pyrus spp.)

The pear (Pyrus spp.) is one of the most important temperate fruit crops. The technique of adventitious shoot regeneration from leaves is considered to be one of the shortcuts in the research on pear genetic modification and cellular engineering, which, however, has not been widely used. As the regeneration frequency of pear leaves is usually very low, the research on adventitious shoot regeneration from pear leaves is eagerly needed. In this experiment, the factors affecting shoot and bud regeneration from the leaves of ‘Zhongli 1’ pear were studied, and an efficient protocol for shoot regeneration was established. The results showed that different types of basic media, different combinations of plant growth regulators, leaf placement on medium, periods of dark culture and the use of silver nitrate (AgNO₃) on culture media all significantly affected the adventitious shoot regeneration frequency of ‘Zhongli 1’ pear. The details are as follows: (1) Among three kinds of basic media, NN69 was better for ‘Zhongli 1’ shoot regeneration, followed by half (½) MS, while full MS had no effect on shoot regeneration; (2) Thidiazuron (TDZ) was better than 6-benzylaminopurine (6-BA) for ‘Zhongli 1’ regeneration, with an optimal concentration of 1.5 mg · L^?1, and the regeneration rate under this concentration could reach 85%, with 2.72 buds per leaf. 0.5 mg · L^?1 indole-3-butyric acid (IBA), which induced a higher regeneration frequency, was a better choice for pear regeneration compared with 0.3 mg · L^?1 naphthaleneacetic acid (NAA). Among the different combinations of plant growth regulators, TDZ + IBA was better for inducing high regeneration frequency; (3) The abaxial surface of leaves touching the medium was beneficial for leaves to uptake nutrients from the medium, and because of that, the regeneration frequency of leaves was significantly higher than that of leaves touching the medium with their adaxial surfaces (obverse side of leaf); (4) Dark culture was necessary for bud regeneration, and the best duration for dark culture of ‘Zhongli 1’ pear was 21 days; (5) The addition of 1.0 mg · L^?1 AgNO₃ into the culture medium could promote adventitious shoot regeneration significantly. A high adventitious shoot regeneration frequency was obtained in this research, which will be beneficial for further research on efficient and stable in vitro plant regeneration systems and genetic modification of pear.     

六出花瓶插过程中乙烯代谢特征及其调控效应

为了探究六出花瓶插过程中乙烯代谢特征及其调控效应,为六出花切花保鲜剂的研发提供理论依据,以花苞期的六出花切花为供试材料,在基础瓶插液( 1 %蔗糖 + 200 mg·L^-1 8-羟基喹啉)中分别加入20 mg·L^-1乙烯利(ETH)、4 mg·L^-1纳米银(NS)、75 mg·L^-1水杨酸(SA)和10 mg·L^-1精胺(SP)配制瓶插液处理六出花切花,分析瓶插过程中切花形态、生理和基因表达3个层面的指标变化。结果表明：（1）含有乙烯利的瓶插液处理显著缩短了六出花切花瓶插寿命,含有纳米银、水杨酸、精胺的瓶插液处理均能延长六出花切花瓶插寿命,其中以含有4 mg·L^-1纳米银的瓶插液处理效果最佳,延长了瓶插期 2 d。（2）含有乙烯利的瓶插液处理显著加速了花枝鲜样质量和花瓣可溶性糖、可溶性蛋白含量的降低,增加了花瓣丙二醛含量和相对电导率,显著提高了花瓣乙烯代谢水平。含有纳米银、水杨酸、精胺的瓶插液处理六出花切花均不同程度地延缓了花枝鲜样质量和花瓣可溶性糖、可溶性蛋白含量的降低,抑制了丙二醛含量和相对电导率的升高,降低了乙烯代谢水平。（3）含有乙烯利的瓶插液处理增加了开花各时期乙烯合成及信号转导关键基因（AlACO、AlACS、AlCTR1、AlERS1和AlEIN3）的表达量;纳米银、水杨酸和精胺均能不同程度地抑制乙烯合成及信号转导关键基因的表达。研究结果显示六出花为乙烯末期上升型花卉,外源施加乙烯抑制剂可以延缓六出花切花衰老,提高瓶插品质,其中以4 mg·L^-1的纳米银效果最佳。     

Modulation of defence responses by improving photosynthetic activity,antioxidative metabolism,and vincristine and vinblastine accumulation in <Emphasis Type="Italic">Catharanthus roseus</Emphasis> (L.) G. Don through salicylic acid under water stress

A pot experiment was conducted to find out whether the foliar spray of salicylic acid (SA) could successfully ameliorate the adverse effects of water stress on periwinkle. Pots were irrigated with ground water regularly as a control and other treatments were given as 15 and 30 days interval drought (DID) at 30 DAS. Plants were uprooted randomly at 46 and 61 DAS, washed carefully and separated into root, stem and leaf for analyses. A uniform concentration (10⁻⁵ mol/l) of salicylic acid (SA) was applied as a foliar spray at the vegetative stage. Water stress significantly reduced the growth attributes including plant height, leaf-area index, shoot and root fresh weights, shoot and root dry weights. Long term water stress led to a gradual decrease in photosynthetic parameters and activities of nitrate reductase and carbonic anhydrase. Ascorbic acid, total alkaloids and antioxidant enzymes: superoxide dismutase, catalase and peroxidase increased in stress faced plants. Foliar application of SA (10⁻⁵ M) reduced the damaging effect of stress on plant growth and accelerated the restoration of growth processes. It not only improved the growth parameters but also partially reversed the effects of salinity. Total alkaloid content was improved by SA application both in unstressed and stressed plants. Foliar spray of SA not only overcame the adverse effect of stress but also improved the content of vincristine and vinblastine in stressed plants.     

化肥配施生物炭对稻田田面水氮磷流失风险影响

在控制外源氮输入相同的前提下,通过大田试验研究生物炭部分替代化肥作为底肥,不同生物炭施用量(5、10、20 t·hm~(-2))对水稻生长期内稻田田面水氮磷迁移转化特征的影响。研究结果表明:各处理的田面水总氮、硝氮、铵氮浓度在施肥后第3 d达到最高,然后迅速下降,并逐渐稳定;田面水总磷浓度在施肥后2~4 d内增幅较小,而后迅速下降至稳定,施加生物炭对田面水总磷的影响不大;可溶性磷浓度在施肥后2~4 d内处于平稳下降的状态,之后迅速下降至稳定。稻田施肥后10 d内是控制氮磷流失的最佳时段。采用生物炭代替部分化肥的施肥方式,在一定范围内能降低稻田田面水的氮磷浓度,稻田退水氮、磷的输出负荷分别减少了39%~50%和38%~50%,显著提高了水稻生态效益。通过综合效益评估可知,施加5 t生物炭代替化肥是综合效益最高的施肥方法,该施肥方式下氮、磷的年输出负荷分别为16.83、1.89 kg·hm~(-2)。     

Screening of Optimal Differentiation Medium toLonicera edulis

An excellent Lonicera edulis strain, L1-8 that was bred by Northeast Institute of Geography and Agro-ecology, Chinese Academy of Sciences, was used as material in this research. The axillary buds of its dormant branches were used as explants. A fourfactor and four-level orthogonal test was designed in order to choose the best differentiation medium for providing the technical support of Lonicera edulis micropropagation. The results showed that the culture medium and concentration of 6-BA were the main factors, and the best differentiation condition was MS culture medium containing 2.0 mg · L-1 6-BA, 0.3 mg · L-1 IBA and 1.5 mg · L-1 GA3.     

富里酸对土壤中DnBP的降解及微生物活性的影响

为探究富里酸对有机污染物DnBP的降解和土壤微生物活性的作用,采用土壤培养试验,研究了FA对土壤中DnBP的降解动力学过程及土壤微生物活性的影响规律。结果表明,FA的添加缩短了DnBP的降解半衰期,FA各处理组的半衰期为对照处理的48.1%~67.7%;FA促进了土壤的基础呼吸,40mg g-1处理组促进作用最强,最大CO2释放量为空白对照组的40.87倍;而过氧化氢酶活性则表现出了低浓度FA(10~20mg g-1)促进,高浓度FA(40~160mg g-1)抑制的变化趋势;FA对土壤脱氢酶活性主要表现为促进作用,40~160mg g-1FA对脱氢酶活性促进作用显著。相关分析结果表明FA用量与土壤中DnBP降解率呈正相关,与过氧化氢酶活性呈极显著负相关,与土壤呼吸强度、脱氢酶活性呈极显著正相关。通径分析结果表明FA用量与土壤中DnBP降解率之间有较强的直接效应,还与土壤呼吸强度、土壤酶之间存在间接效应,从而影响DnBP的降解。     

Influences of nitrogen fertilizer application rates on radish yield, nutrition quality, and nitrogen recovery efficiency

Radishes (Raphanus sativus L.) were grown in plastic pots in a screenhouse to investigate the influences of nitrogen fertilizer application rates (NFAR) on yield, nitrate content, nitrate reductase activity (NR), nutrition quality, and nitrogen recovery efficiency (NRE) at commercial mature stage. Five N-rate treatments, 0.644, 0.819, 0.995, 1.170, and 1.346 g·pot⁻¹, were set up in the screenhouse pot experiments, and nitrogen fertilizer (unlabeled N and ¹⁵N-labeled fertilizer) was applied as basal dressing and topdressing, respectively. The results indicated that the fresh and dry weight yields of radish increased with the increase of NFAR at the range of 0.099 to 0.180 g N·kg⁻¹ soil, decreased at 0.207 g N·kg⁻¹ soil, and accordingly there was a significant quadratic relationship between the fresh and dry weight yields of radish and the NFAR. At the high addition of urea-N fertilizer, the nitrate content accumulated in the fleshy roots and leaves due to the decline in NR activity. From 0.644 to 0.819 g N·pot⁻¹ NR increased most rapidly, the highest NR activity occurred at 0.819 g N·pot⁻¹, and the lowest NR activity happened at 1.346 g N·pot⁻¹. Soluble sugar and ascorbic acid initially increased to the highest value and then decreased, and, contrarily, crude fiber rapidly decreased with the increase of NFAR. Total N uptake (TNU), N derived from fertilizer (N_dff), and N derived from soil (N_dfs) in radish increased, except that N_dfs relatively and slightly decreased at the rate of 0.207 g N·kg⁻¹soil. The ratio of Ndff to TNU increased, but the ratio of Ndfs to TNU as well as NRE of N fertilizer decreased with the increase of NFAR. Therefore, the appropriate NFAR should be preferably recommended for improving the yields and nutrition qualities of radish and NRE of N fertilizer. These authors contributed equally to this work     

水杨酸对黄瓜幼苗生理的影响

为了明确不同浓度外源水杨酸(Salicylic acid,SA)处理对黄瓜幼苗生长的影响,以黄瓜品种“中农203号”(Cucumis satiuns L.cv.ZhongNong 203)为试材,采用水培方法设计根际0.05 mmol/L、0.1mmol/L和0.5mmol/L SA 处理,研究黄瓜幼苗对SA的生理响应.结果表明:黄瓜幼苗叶片净光合速率(Pn)、胞间 CO2浓度(Ci)、气孔导度(Gs)、蒸腾速率(Tr)、叶绿素含量、植株含水量、电解质渗透率和MDA含量对SA的响应存在明显的浓度依存性和时间效应.0.05 mmol/L和0.1mmol/L SA处理增加了Ci和叶绿素含量;降低了电解质渗透率和MDA含量,提高了Pn,以0.1mmol/L SA处理效果最佳;而0.5 mmol/L SA处理降低了Ci和叶绿素含量,提高了Tr,最终导致Pn下降.结果表明,适宜浓度的SA处理有利于幼苗的生长.     

浆果生长期水杨酸和乙烯利处理对梅鹿辄葡萄品质的影响

为了探索浆果生长期不同质量浓度的水杨酸和乙烯利处理对酿酒葡萄果实品质的影响,以贺兰山东麓产区玉泉营4a生梅鹿辄葡萄为试材,在葡萄浆果生长期设置50、100、200 mg·L-1的水杨酸和300、600、900mg·L-1的乙烯利处理,研究不同果实的可溶性固形物、总糖、总酸、总酚、单宁以及总花色素含量等品质指标的影响,并运用主成分分析法对各指标进行综合分析评价。结果表明,不同处理可有效提高果实品质,且影响显著。其中经600 mg·L-1乙烯利处理果实的总糖、总酚、总花色素分别较对照提高10.76%、22.32%、39.96%;尤其经100mg·L-1水杨酸处理的效果更明显,果实的总糖、总酚、单宁较对照分别提高13.64%、44.24%、44.74%。说明,100mg·L-1水杨酸及600mg·L-1乙烯利处理最有利于酿酒葡萄果实品质提高。     

水杨酸增强黄瓜幼苗耐盐性的生理机制

【目的】明确外源水杨酸（Salicylic acid，SA）对黄瓜（Cucumis sativus L.）幼苗耐盐性的作用。【方法】采用SA根际注射和叶面喷施相结合的方法，研究了NaCl胁迫下黄瓜幼苗对外源SA的生理响应。【结果】外源SA促进了NaCl胁迫下黄瓜叶片可溶性糖和Pro的积累，最高分别使两者含量提高了110.4%和82.2%。SA处理提高了NaCl胁迫下黄瓜叶片SOD、POD、CAT活性，降低了非胁迫条件下黄瓜叶片SOD活性。SA使NaCl胁迫下黄瓜幼苗保持较高的含水量，并与单一NaCl胁迫对照达到极显著差异水平（n=10，P＜0.01）。施用外源SA后，与单一NaCl胁迫相比黄瓜叶片MDA含量和原生质膜电解质渗透率分别降低了30.9%和79.9%。SA处理能够显著改善NaCl胁迫下黄瓜植株的株高、茎粗和干物质积累（n=10，P＜0.05），有效地缓解NaCl对黄瓜幼苗生长的抑制作用。【结论】外源SA可以通过提高植株渗透调节能力和抗氧化能力，维持植株水分平衡，保护膜结构和功能，减轻NaCl对黄瓜幼苗的胁迫伤害。     

落叶果树芽休眠期的呼吸变化

本文系统论述了落叶果树芽在休眠期间呼吸强度及呼吸途径的动态变化，分析了不同因素对呼吸代谢的影响，以期通过呼吸代谢的调控而调节果树休眠的进程。简略探讨了落叶果树芽自然休眠的酶学和膜功能研究，并对今后的研究发展作了展望。     

裂盖马鞍菌甲醇提取物清除自由基活性的研究

采用DPPH法、水杨酸法、邻苯三酚自氧化法分别测定裂盖马鞍菌甲醇提取物清除DPPH自由基、羟自由基和超氧自由基的能力。结果表明,在实验条件下,裂盖马鞍菌甲醇提取物对超氧阴离子、羟自由基、DPPH自由基均有不同程度的抑制作用,最高清除活性分别达72.72%、54.90%、92.08%。因此裂盖马鞍菌甲醇提取物具有一定的体外抗氧化活性。