共查询到20条相似文献,搜索用时 15 毫秒
1.
Opiates and pain pathways: demonstration of enkephalin synapses on dorsal horn projection neurons 总被引:1,自引:0,他引:1
M A Ruda 《Science (New York, N.Y.)》1982,215(4539):1523-1525
The participation of the opiate peptide enkephalin in the neural circuitry of the dorsal horn was examined at the light and ultrastructural level through the use of the combined techniques of immunocytochemistry and retrograde transport of horseradish peroxidase. Enkephalin immunoreactive axonal endings made direct synaptic contact with the soma and proximal dendrites of dorsal horn thalamic projection neurons. This observation demonstrates that one major synaptic site of enkephalin modulation of the transfer of nociceptive information in the dorsla horn is on the projection neurons themselves.U 相似文献
2.
The histochemical reaction for phosphorylase is completely lost from anterior horn neurons rich in phosphorylase within 72 hours after proximal or distal axonal section. Using this new type of axonal reaction as a marking technique in the anterior horn of the seventh lumbar spinal cord segment of the cat, we demonstrated that (i) alpha motor neurons of slow twitch motor units, like those of fast twitch motor units, are rich in phosphorylase and poor in succinate dehydrogenase, and (ii) interneurons and Renshaw neurons are rich in succinate dehydrogenase and poor in phosphorylase. Gamma motor neurons, because of their small size, are considered to be rich in succinate dehydrogenase and poor in phosphorylase. Thus, anterior horn neurons capable of higher firing frequencies (Renshaw neurons, interneurons, and gamma motor neurons) are richer in mitochondrial oxidative enzyme activity as marked by succinate dehydrogenase. Those firing at lower frequencies (both types of alpha motor neurons) are richer in phosphorylase activity and glycogen content and, thus, apparently better equipped for anaerobic glycolysis. 相似文献
3.
Harvey RJ Depner UB Wässle H Ahmadi S Heindl C Reinold H Smart TG Harvey K Schütz B Abo-Salem OM Zimmer A Poisbeau P Welzl H Wolfer DP Betz H Zeilhofer HU Müller U 《Science (New York, N.Y.)》2004,304(5672):884-887
Prostaglandin E2 (PGE2) is a crucial mediator of inflammatory pain sensitization. Here, we demonstrate that inhibition of a specific glycine receptor subtype (GlyR alpha3) by PGE2-induced receptor phosphorylation underlies central inflammatory pain sensitization. We show that GlyR alpha3 is distinctly expressed in superficial layers of the spinal cord dorsal horn. Mice deficient in GlyR alpha3 not only lack the inhibition of glycinergic neurotransmission by PGE2 seen in wild-type mice but also show a reduction in pain sensitization induced by spinal PGE2 injection or peripheral inflammation. Thus, GlyR alpha3 may provide a previously unrecognized molecular target in pain therapy. 相似文献
4.
Inflammation, trauma, or nerve injury may cause enduring hyperalgesia, an enhanced sensitivity to painful stimuli. Neurons in lamina I of the spinal dorsal horn that express the neurokinin 1 receptor for substance P mediate this abnormal pain sensitivity by an unknown cellular mechanism. We report that in these, but not in other nociceptive lamina I cells, neurokinin 1 receptor-activated signal transduction pathways and activation of low-threshold (T-type) voltage-gated calcium channels synergistically facilitate activity- and calcium-dependent long-term potentiation at synapses from nociceptive nerve fibers. Thereby, memory traces of painful events are retained. 相似文献
5.
Input resistance, electrical excitability, and size of ventral horn cells in cat spinal cord 总被引:5,自引:0,他引:5
D Kernell 《Science (New York, N.Y.)》1966,152(729):1637-1640
Experiments on cat lumbosacral alpha motoneurones showed that, in comparison with cells possessing rapidly conducting axons, the cells with slowly conducting axons have the higher input resistance, that they need weaker stimulating currents to reach the threshold for repetitive firing, and that they need a relatively larger increment in current strength for a given increase in firing rate. Measurements of the number and diameters of dendritic trunks gave larger values for the larger cell bodies. The discussion deals with the interrelation between cell geometry, electrical properties, and the reflex action of alpha motoneurones. 相似文献
6.
Emery EC Young GT Berrocoso EM Chen L McNaughton PA 《Science (New York, N.Y.)》2011,333(6048):1462-1466
The rate of action potential firing in nociceptors is a major determinant of the intensity of pain. Possible modulators of action potential firing include the HCN ion channels, which generate an inward current, I(h), after hyperpolarization of the membrane. We found that genetic deletion of HCN2 removed the cyclic adenosine monophosphate (cAMP)-sensitive component of I(h) and abolished action potential firing caused by an elevation of cAMP in nociceptors. Mice in which HCN2 was specifically deleted in nociceptors expressing Na(V)1.8 had normal pain thresholds, but inflammation did not cause hyperalgesia to heat stimuli. After a nerve lesion, these mice showed no neuropathic pain in response to thermal or mechanical stimuli. Neuropathic pain is therefore initiated by HCN2-driven action potential firing in Na(V)1.8-expressing nociceptors. 相似文献
7.
The centrifugal fibers in the pigeon retina end in the inner nuclear layer and form two kinds of terminals, convergent and divergent. In the inner nuclear layer the fibers synapse with amnacrine and displaced ganglion cells. Because of their great number and their even distribution these fibers appear to constitute a system for the localized centrifugal control of the retinal functions. 相似文献
8.
Synaptic rearrangement during development is a characteristic of the vertebrate nervous system and was thought to distinguish vertebrates from the invertebrates. However, examination of the wind-sensitive cercal sensory system of the cricket demonstrates that some identified synaptic connections systematically decrease in strength as an animal matures, while others increase in strength over the same period. Moreover, a single sensory neuron could increase the strength of its synaptic connection with one interneuron while decreasing the strength of its connection with another interneuron. Thus, rather than being a hallmark of the vertebrate nervous system, synaptic rearrangement is probably characteristic of the development of many if not all nervous systems. 相似文献
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10.
Verhage M Maia AS Plomp JJ Brussaard AB Heeroma JH Vermeer H Toonen RF Hammer RE van den Berg TK Missler M Geuze HJ Südhof TC 《Science (New York, N.Y.)》2000,287(5454):864-869
Brain function requires precisely orchestrated connectivity between neurons. Establishment of these connections is believed to require signals secreted from outgrowing axons, followed by synapse formation between selected neurons. Deletion of a single protein, Munc18-1, in mice leads to a complete loss of neurotransmitter secretion from synaptic vesicles throughout development. However, this does not prevent normal brain assembly, including formation of layered structures, fiber pathways, and morphologically defined synapses. After assembly is completed, neurons undergo apoptosis, leading to widespread neurodegeneration. Thus, synaptic connectivity does not depend on neurotransmitter secretion, but its maintenance does. Neurotransmitter secretion probably functions to validate already established synaptic connections. 相似文献
11.
Abrahamsen B Zhao J Asante CO Cendan CM Marsh S Martinez-Barbera JP Nassar MA Dickenson AH Wood JN 《Science (New York, N.Y.)》2008,321(5889):702-705
Peripheral pain pathways are activated by a range of stimuli. We used diphtheria toxin to kill all mouse postmitotic sensory neurons expressing the sodium channel Nav1.8. Mice showed normal motor activity and low-threshold mechanical and acute noxious heat responses but did not respond to noxious mechanical pressure or cold. They also showed a loss of enhanced pain responses and spontaneous pain behavior upon treatment with inflammatory insults. In contrast, nerve injury led to heightened pain sensitivity to thermal and mechanical stimuli indistinguishable from that seen with normal littermates. Pain behavior correlates well with central input from sensory neurons measured electrophysiologically in vivo. These data demonstrate that Na(v)1.8-expressing neurons are essential for mechanical, cold, and inflammatory pain but not for neuropathic pain or heat sensing. 相似文献
12.
We have recorded from the granular layer of the turtle cerebellum extracellular unitary potentials that appear to reflect pre- and postsynaptic events at the synapse between a single swelling of a mossy fiber and the dendritic tips of several granule cells. The presynaptic component is an all-or-none potential. It can be directly activated by spinal stimulation and is unaltered by repetitive activity or by high concentrations of magnesium. The postsynaptic component is a graded potential. It follows the presynaptic component by approximately 1 millisecond and is depressed by repetitive activity and by high concentrations of magnesium. The recording of large potentials produced by the flow of postsynaptic current within a single glomerulus suggests powerful transmission. Electron micrographs demonstrate large cerebellar glomeruli in the turtle and a substantial accumulation of mitochondria in the dendritic tips of granule cells. 相似文献
13.
Eddy JA 《Science (New York, N.Y.)》1974,184(4141):1035-1043
14.
Synaptic reorganization in the hippocampus induced by abnormal functional activity 总被引:55,自引:0,他引:55
Abnormal functional activity induces long-lasting physiological alterations in neural pathways that may play a role in the development of epilepsy. The cellular mechanisms of these alterations are not well understood. One hypothesis is that abnormal activity causes structural reorganization of neural pathways and promotes epileptogenesis. This report provides morphological evidence that synchronous perforant path activation and kindling of limbic pathways induce axonal growth and synaptic reorganization in the hippocampus, in the absence of overt morphological damage. The results show a previously unrecognized anatomic plasticity associated with synchronous activity and development of epileptic seizures in neural pathways. 相似文献
15.
Drdla-Schutting R Benrath J Wunderbaldinger G Sandkühler J 《Science (New York, N.Y.)》2012,335(6065):235-238
Painful stimuli activate nociceptive C fibers and induce synaptic long-term potentiation (LTP) at their spinal terminals. LTP at C-fiber synapses represents a cellular model for pain amplification (hyperalgesia) and for a memory trace of pain. μ-Opioid receptor agonists exert a powerful but reversible depression at C-fiber synapses that renders the continuous application of low opioid doses the gold standard in pain therapy. We discovered that brief application of a high opioid dose reversed various forms of activity-dependent LTP at C-fiber synapses. Depotentiation involved Ca(2+)-dependent signaling and normalization of the phosphorylation state of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors. This also reversed hyperalgesia in behaving animals. Opioids thus not only temporarily dampen pain but may also erase a spinal memory trace of pain. 相似文献
16.
Eyes were removed from rats shortly after birth, when there are few formed synapses in the colliculus. It was found that synaptogenesis continues to give a near-normal ratio of terminals containing either spheroidal or flattened vesicles. After eye removal in adult rats, however, reinvasion of synaptic sites vacated by degenerate optic terminals occurs, with an incomplete return toward a normal proportion of synaptic types. 相似文献
17.
Synaptic transmission between photoreceptors and horizontal cells in the turtle retina 总被引:18,自引:0,他引:18
Low calcium, high magnesium, and cobalt hyperpolarize the horizontal cell membrane and suppress the response to light, but only partially affect the response of receptor cells. These observations are consistent with the interpretation that a depolarizing transmitter is released by photoreceptors in darkness. The hyperpolarizing response to light of the horizontal cells would then result from a reduction in the amount of transmitter released. 相似文献
18.
Freeze-etched neuropil of the cat subfornical organ was examined with the electron microscope for synaptic vesicles. Round vesicles were found exclusively in both unfixed and aldehyde-fixed specimens. Range of diameters and histograms failed to differ significantly between freeze-etched and conventionally prepared material. The mnode of distribution of diameters was approximately 500 angstroms. Round stomata (approximately 350 angstromns in diameter) were found at the outer surface of the plasmalemma of nerve terminials; they are interpreted as pinocytotic vesicles. 相似文献
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Synaptic potentials recorded in cell cultures of nerve and muscle 总被引:11,自引:0,他引:11
G D Fischbach 《Science (New York, N.Y.)》1970,169(952):1331-1333
Initially dissociated spinal cord and muscle cells derived from chick embryos differentiate sufficiently in tissue culture to form functional synaptic contacts. Spontaneous and evoked potentials recorded with intracellular microelectrodes resemble synaptic responses of adult spinal cord and neuromuscular junctions. 相似文献