黔西县几个种猪场生猪疫病血清学调查报告

采用正向间接血凝（IHA）试验随机对3个1000头以上种猪场的90份血清,进行了猪口蹄疫（FMD）、猪瘟（HC）血清学监测;同时还采用一步法猪瘟（HC）、猪繁殖与呼吸综合征（PRRS）疫病病毒反转录（PCR）对血清进行了检测。结果表明,种猪场的FMD、HC免疫合格率分别为85.56%和87.78%,均达到国家农业部规定70.0%的标准;用PCR法检测HC、PRRS阳性检出数为0。     

黔西县规模养猪场生猪疫病血清学调查报告

采用正向间接血凝(IHA)试验对黔西县7个500头以上规模养猪场的175份血清,进行了猪口蹄疫(FMD)、猪瘟(HC)血清学监测;同时采用酶联免疫吸附试验(ELISA)进行了猪繁殖与呼吸综合征(PRRS)血清学检测。结果显示:规模养猪场FMD、HC免疫合格率分别为72.0%和79.4%,均达到国家农业部规定70.0%的标准;PRRS阳性检出率为8.0%,表明该疫病在黔西县已有感染,应予以重视,加强防治。     

猪瘟（HC）、口蹄疫（FMD）、猪繁殖与呼吸综合征（PRRS）疫苗同时分点免疫的效果分析

选择日龄相近、饲养条件一致、健康状况良好的断奶仔猪150头,随机分为A、B、C三组,A组按传统免疫程序接种HC、FMD疫苗;B组按传统免疫程序接种HC、FMD、PRRS疫苗;C组则同时分点接种HC、FMD、PRRS疫苗。经检测,三种疫苗同时分点接种对动物本身不会产生任何免疫副作用;HCV抗体检测合格率首免后达到68.0%,二免后达到91.7%;FMD抗体检测合格率首免后达到80.0%,二免后达到81.3%;新生乳猪和断乳仔猪发生PRRS的死亡率分别从原来的17.5%、17.2%下降到0%。     

规模化猪场主要传染病抗体水平检测及分析

2003年11月,笔者在武汉市郊某规模化猪场对种猪、育肥猪和哺乳仔猪群随机抽检153份血清进行了猪瘟(HC)、口蹄疫(FMD)、猪传染性胸膜肺炎(APP)、蓝耳病(PRRS)和猪圆环病毒(PCV)等主要传染病的抗体检测,现将结果报道如下。     

2018—2021年上海市嘉定区规模猪场主要疫病抗体监测

为了解上海市嘉定区规模猪场口蹄疫(FMD)、猪瘟(CSF)、猪繁殖与呼吸综合征(PRRS)、猪伪狂犬病(PR)和猪流行性腹泻(PED)等疫病的免疫及流行状况,对嘉定区现有的2个规模猪场2018—2021年的检测数据进行汇总分析.结果显示:嘉定区规模猪场FMD、CSF、PRRS、PR免疫抗体平均合格率分别为91.8％、8...     

猪口蹄疫、猪瘟、高致病性蓝耳病三种疫苗不同组合同时分点免疫的效果观察

为了寻求猪瘟(CSF)、猪口蹄疫(FMD)和猪高致病性蓝耳病(PRRS)疫苗联合注射的新型免疫程序,本研究采用这3种疫苗的不同组合进行免疫,将40～50日龄健康仔猪133头分为7组,观察其免疫反应,并经ELISA试验检测血清抗体效价来评价其免疫效果。结果得出:3种疫苗同时分点免疫,免疫副反应率比较低,特别是进行二免、三免后抗体效价更高,适宜推广。     

猪瘟、猪蓝耳病、猪口蹄疫免疫抗体检测分析

为了实时掌握伊州区猪瘟(Classical swine fever, CSF)、猪蓝耳病(Porcine reproductive and respiratory syndrome, PRRS)、口蹄疫(Foot-and-mouth disease virus,FMD)抗体免疫状况,研究采用ELISA方法对该区的156份血样进行了猪瘟(CSF)、猪蓝耳病(PRRS)、口蹄疫(FMD)抗体检测。结果表明:规模场猪群猪瘟、猪蓝耳病、口蹄疫抗体合格率分别69.23%、70.51%、79.49%,其中猪瘟免疫效果不理想,必须实施紧急补免;散养户猪群猪瘟、猪蓝耳病、口蹄疫抗体合格率分别80.77%、79.49%、96.15%,免疫效果均较理想。此次检测为更进一步做好全区猪群CSF、PRRS和FMD的有效防控提供了基本参考和科学依据。     

甘肃省部分规模化猪场主要疫病抗体监测及动态分析

本试验采用乳胶凝集试验(LAT)、正向间接血凝试验(IHB) 和酶联免疫吸附试验(ELISA)检测了甘肃省6个规模化猪场233份血清9种疫病抗体水平。检测结果显示：猪瘟(HC)、猪O型口蹄疫(FMD)、猪传染性胸膜肺炎(APP)、猪伪狂犬(PR)、猪细小病毒病(PP)、猪萎缩性鼻炎(AR)、猪日本乙型脑炎(JB)、 蓝耳病(PRRS)和猪圆环病毒2型(PCV-2)抗体阳性率分别为78.54%、62.23%、44.64%、35.62%、20.17%、14.59%、57.08%、 63.52%和90.13%。结果表明：PCV 2免疫效果好,其余8种均不理想。     

2015～2019年蒙自市猪瘟、蓝耳病、猪O型口蹄疫免疫抗体监测分析

为全面掌握蒙自市生猪防疫质量和免疫效果,2015～2019年对全市11个乡镇的44个规模养殖场和106户散养户共采集血清974份,采用酶联免疫吸附试验分别对猪瘟(CSF)、蓝耳病(PRRS)、猪O型口蹄疫(FMD)的免疫抗体进行监测。结果显示:2015～2019年,CSF、PRRS、FMD总体免疫抗体阳性率分别为80.86%、74.59%、66.37%;春防免疫中FMD免疫不合格,秋防免疫中PRRS、FMD免疫不合格,其余均为合格;规模猪场与散养户免疫抗体FMD均为不合格,其余均为合格,且规模猪场阳性率均高于散养户。CSF、PRRS、FMD均免疫合格的乡镇有3个,免疫均不合格的乡镇有2个;CSF、PRRS免疫合格的乡镇有4个;CSF、FMD免疫合格的乡镇有1个;仅有PRRS免疫合格的乡镇有1个。     

陕西省部分猪场5种疫病抗体及病原的检测

为调查2019年陕西省规模化猪场中口蹄疫(FMD)、猪繁殖与呼吸综合征(PRRS)、猪瘟(CSF)、猪圆环病毒2型(PCV2)和猪伪狂犬病(PR)的免疫状况及病原感染情况,采用间接ELISA对送检的20233份血清样品进行抗体检测,采用PCR/RT-PCR方法对送检的1633份病料样品进行病原检测.结果表明,检测口蹄疫...     

猪群高热症的血清流行病学调查研究

采用血清学试验对贵阳市花溪区某种猪场的95份血清样本进行猪繁殖障碍性疫病血清学检测,以了解猪群免疫水平及疫病感染状况.结果显示,猪群O型口蹄疫（O-FMD）、猪瘟（HC）、猪繁殖与呼吸障碍综合征（PRRS）、猪伪狂犬病（PR）免疫合格率分别为100％ （33/33）、62.9％（39/62）、14.5％ （9/62）、61.3％ （38/62）,HC、PRRS和PR免疫合格率均未达到农业部要求;通过比较母猪免疫抗体与仔猪母源抗体水平相关性,发现HC与PR首免后抗体保护期维持较短,PRRS免疫不合格;猪群猪圆环病毒病（PC）、猪细小病毒病（PP）隐性感染抗体阳性率分别为70.5％ （67/95）、82.1％（78/95）,隐性感染情况严重.血清流行病学调查结果表明,该猪场应改进HC、PR免疫程序,立即对全群进行PRRS补免,并要高度重视PC和PP的防控。     

猪瘟脾淋苗、猪O型口蹄疫和猪蓝耳病灭活苗两两联合应用的免疫效果观察

将猪瘟弱毒脾淋疫苗、猪O型口蹄疫灭活疫苗(Ⅱ)和高致病性猪蓝耳病灭活疫苗采用两两组合的方式同时分部位免疫35日龄左右的健康仔猪,观察并记录其免疫反应。在疫苗免疫后0、4、14、21d和28d无菌取猪血清,用ELISA检测免疫后三种疫苗抗体水平的动态变化。试验结果表明:猪瘟和猪口蹄疫疫苗同时免疫,效果较好;猪口蹄疫和高致病性猪蓝耳病疫苗同时免疫,效果较差;猪瘟疫苗和高致病性猪蓝耳病疫苗同时免疫,效果不明显,还需进一步验证。     

Efficacy of Fostera PRRS modified live virus vaccine against a Canadian heterologous virulent field strain of porcine reproductive and respiratory syndrome virus

Vaccination is a useful option to control infection with porcine reproductive and respiratory syndrome virus (PRRSV), and several modified live-PRRSV vaccines have been developed. These vaccines have shown some efficacy in reducing the incidence and severity of clinical disease as well as the duration of viremia and virus shedding but have failed to provide sterilizing immunity. The efficacy of modified live-virus (MLV) vaccines is greater against a homologous strain compared with heterologous PRRSV strains. The objective of this study was to evaluate the efficacy of Fostera PRRS MLV vaccine in protecting against challenge with a heterologous field strain widely circulating in the swine herds of eastern Canada. Forty-six piglets were divided into 4 groups: nonvaccinated-nonchallenged; nonvaccinated-challenged; vaccinated-challenged; and vaccinated-nonchallenged. The animals were vaccinated at 23 d of age with Fostera PRRS and challenged 23 d later with a heterologous field strain of PRRSV (FMV12-1425619). Overall, the vaccine showed some beneficial effects in the challenged animals by reducing the severity of clinical signs and the viral load. A significant difference between nonvaccinated and vaccinated animals was detected for some parameters starting 11 to 13 d after challenge, which suggested that the cell-mediated immune response or other delayed responses could be more important than pre-existing PRRSV antibodies in vaccinated animals within the context of protection against heterologous strains.     

不同类型猪蓝耳病疫苗的体液免疫效果比较

为科学评价不同猪蓝耳病疫苗对生产母猪和仔猪免疫效果,选取11个规模猪场,分别使用猪蓝耳病进口弱毒疫苗、国产弱毒疫苗、普通灭活疫苗、猪高致病性蓝耳病灭活疫苗对生产母猪和仔猪进行免疫,二免28d后,采集免疫猪群的血清,进行免疫抗体检测,结果表明:生产母猪使用不同类型的蓝耳病疫苗免疫后所产生的抗体阳性率均高于仔猪免疫后所产生的抗体阳性率。     

农村散养猪猪瘟疫苗首免及二免效果观察

为探索农村散养猪猪瘟疫苗首次及二次免疫抗体变化规律,选择20头35日龄仔猪,35日龄首次免疫,75日龄二次免疫,用猪瘟正向间接血凝试验监测免疫抗体。结果：猪瘟疫苗首次免疫合格期为40天,二次免疫合格期达到95天以上。     

口蹄疫双佐剂灭活疫苗的研究

在口蹄疫二价灭活疫苗（O型、AsiaⅠ型）常规使用法国SEPPIC公司206佐剂的基础上,设计了一种缓释作用更强的双佐剂成分疫苗。通过MTT法检测淋巴细胞增殖能力、液相阻断ELISA法检测口蹄疫抗体效价及攻毒试验测定PD50来评判该双佐剂疫苗与常规疫苗的效果差异。结果显示,双佐剂疫苗组细胞免疫水平（淋巴细胞刺激指数SI为1.235±0.060）比常规佐剂疫苗组（SI为1.115±0.035）和对照组（SI为1.010±0.045）高,与常规疫苗组相比差异显著（P＜0.05）,与空白对照组相比,差异极显著（P＜0.01）。双佐剂疫苗组O型和AsiaⅠ型抗体与常规佐剂疫苗组相比,全剂量组抗原量较充分,两组抗体水平差距不大;1/3剂量组和1/9剂量组由于抗原量较少和强缓释作用,导致抗体水平明显较低。攻毒保护结果为双佐剂疫苗组略高于常规佐剂疫苗组,前者每头份疫苗AsiaⅠ型为9.0 PD50,O型为11.84 PD50,后者每头份疫苗AsiaⅠ型为9.0 PD50,O型为9.0 PD50。由分析结果可见,双佐剂疫苗可引起较好的细胞免疫应答和缓释作用,达到好的攻毒保护效果。     

Asia I型口蹄疫疫苗效力检验替代方法的初步研究

使用三株针对AsiaⅠ型口蹄疫146S抗原的杂交瘤细胞株(1#G3C2、2#G6B9、10#C2G1)大量生产腹水单克隆抗体,粗提纯化并按一定的比例进行混合。初步建立混合腹水单克隆抗体介导的间接夹心ELISA方法,使用该方法测定AsiaⅠ型病毒灭活抗原、O型病毒灭活抗原、正常BHK21细胞培养液,结果表明该方法特异性好,所测定OD值和AsiaⅠ型抗原稀释倍数呈明显线性关系。用该方法对11批AsiaⅠ型、O型单价或双价成品疫苗破乳抗原进行测定,结果表明不同批次不同企业生产的疫苗完整病毒颗粒抗原含量有差异,进一步完善有望用于AsiaⅠ型口蹄疫成品疫苗中有效颗粒抗原含量的测定,从而发展成一种疫苗效力检验替代方法。     

不同厂家猪O型口蹄疫灭活疫苗免疫效果监测

为准确掌握投放安徽省的A、B、C、D 4个厂家的猪O型口蹄疫灭活疫苗在实际生产中的免疫效果,2008年秋季至2011年春季,共采集了844个猪场10 439份免疫接种后1个月左右血清样,采用液相阻断ELISA检测了O型口蹄疫抗体滴度。结果显示,4个厂家疫苗的免疫接种效果差异明显,B厂疫苗免疫效果最好,D厂疫苗次之;不同年份疫苗的免疫接种效果差异显著,2010年的疫苗免疫接种效果最好。     

Rapid methodology for antigenic profiling of FMDV field strains and for the control of identity, purity and viral integrity in commercial virus vaccines using monoclonal antibodies

Monoclonal antibodies (MAbs) developed against different foot-and-mouth disease virus (FMDV) vaccine strains were extensively used to study any possible antigenic variations during vaccine production in Argentine facilities. Additionally, a typing ELISA using strain specific MAbs was developed to detect potential cross contaminations among FMDV strains in master and working seeds with high specificity and sensitivity and to confirm strains identity in formulated vaccines. This assay was carried out for the South American strains currently in use in production facilities in Argentina (A24/Cruzeiro, A/Argentina/01, O1/Campos and C3/Indaial) and for the strain O/Taiwan, produced only for export to Asia. These non-cross reactive MAbs were also used to analyze the integrity of viral particles belonging to each one of the individual strains, following isolation of 140S virions by means of sucrose density gradients from the aqueous phase of commercial polyvalent vaccines. Antigenic profiles were defined for FMDV reference strains using panels of MAbs, and a coefficient of correlation of reactivity with these panels was calculated to establish consistent identity upon serial passages of master and production seeds. A comparison of vaccine and field strain antigenic profiles performed using coefficients of correlation allowed the rapid identification of two main groups of serotype A viruses collected during the last FMD epidemic in Argentina, whose reactivity matched closely to A/Argentina/2000 and A/Argentina/2001 strains.     

Immunogenicity of foot-and-mouth disease virus type O1 replicated in either monolayer or suspended BHK cell system

The efficacy of vaccines formulated from the 10th passage of foot-and-mouth disease virus (FMDV) type O1 in monolayer baby hamster kidney (BHK) cells and the 8th passage in suspension BHK cells was compared in steers. The vaccines were inactivated with ethylenimine, contained an equal amount of antigen and were emulsified in oil-adjuvant. Six animals were vaccinated with each vaccine. During the challenge of immunity (91 days post-vaccination, DPV), one out of the six steers from the monolayer vaccine group became infected with the challenge virus while none of the six steers from the suspension vaccine group contracted the disease during the test period. The neutralizing antibody titers (means) of the serum samples taken at different DPV also did not suggest significant variation between these vaccines. In addition exposure to FMDV infected animals demonstrated that both vaccines elicited an immune state in the vaccinates.