Cytotoxic antibody in normal human serums reactive with tumor cells from acute lymphocytic leukemia

Serums showing complement-dependent cytotoxic reactions to acute lymphocytic leukemia cells were detected in three normal unimmunized subjects. These serums were reactive with tumor cells from 514 (514 tested) acute lymphocytic leukemia patients, and three (12 tested) patients with acute myelocytic leukemia; they did not react with tumor cells from patients with acute monocytic leukemia (two tested), with chronic lymphocytic leukemia (two tested) or with leukolymphosarcoma (two tested); nor did they react with normal lymphocytes from 52 different donors. These reactive serums appear to recognize antigens primarily associated with acute lymphocytic leukemia.     

高简并引物小温度范围不规则降落PCR在庞大基因家族扩增中的应用（摘要）

[目的]探讨了高简并性引物扩增庞大基因家族基因的特殊方法。[方法]采用常规高简并引物对鲤鱼基因组DNA分析进行了常规PCR扩增、常规降落PCR扩增以及优化后的小温度范围不规则跳跃降落PCR扩增。[结果]采用PCR仅得1条明显条带,得到的基因较少;采用常规降落PCR法仅得到弥散性扩增,无明显条带出现;而采用小温度范围的不规则跳跃降落PCR则得到了得到了3条明显条带和多个基因,扩增结果理想。[结论]为庞大基因家族扩增条件中的优化与选择提供了理论依据。     

CHROMATOGRAPHIC HETEROGENEITY OF RABBIT SERUM AMYLASE

A chromatograph of normal rabbit serum shows three major peaks of amylase activity. Material associated with each peak differed in composition of serum protein, electrophoretic distribution of amylolytic activity, optimum pH for activity and Michaelis-Menten constants for starch hydrolysis. These data suggest but do not prove conclusively that normal rabbit serum contains at least three molecular forms of amylase.     

亚致死剂量苏云金杆菌蜡螟亚种对大蜡螟幼虫磷酸酶活力的影响

本文研究了亚致死剂量苏云金杆菌蜡螟亚种对大蜡螟幼虫磷酸酶活力的影响。结果表明,经苏云金杆菌蜡螟亚种(Bacillus thuringiensis galleriae)晶体蛋白处理后大蜡螟幼虫肠道酸性磷酸酶比活力升高,电泳分析表明肠道碱性磷酸酶活力降低,同功酶谱无明显变化。组织化学染色结果也表明,中肠细胞碱性磷酸酶活力下降。但幼虫血淋巴碱性磷酸酶活力及同功酶谱都没有明显变化。     

Three gamma-globulins in normal human serum revealed by monkey precipitins

Precipitating antibodies specific for three normal human gamma-globulins of relatively slow electrophoretic mobility were prepared in monkeys and demonstrated by immtinoelectrophoresis in conjunction with absorption techniques in which two myeloma globulins were used as absorbents.     

Athens variant of glucose-6-phosphate dehydrogenase

A variant of glucose-6-phosphate dehydrogenase (G6PD), characterized by slower than normal electrophoretic migration and associated with mild deficiency of G6PD in the red cells, was detected in two unrelated Greek males. Electrophoretic, chromatographic, and enzymologic study indicated that the new mutant is structurally different from normal G6PD (B+) and from the Mediterranean variant associated with red-cell enzyme deficiency (B-). Convincing electrophoretic separation of the new variant from the normal B+ and the Mediterranean B- enzymes was achieved only by detailed electrophoretic study in different buffer systems and conditions.     

Lineage-specific requirement of c-abl function in normal hematopoiesis

Structural abnormalities of the c-abl proto-oncogene are found in hematopoietic cells of more than 90 percent of individuals with chronic myelogenous leukemia. Therefore c-abl may be important in normal as well as malignant hematopoiesis. Normal human hematopoietic progenitor cells were exposed to three different c-abl sense or antisense oligodeoxynucleotides, and the effects on myeloid and erythroid colony formation were examined. The c-abl antisense oligodeoxynucleotides inhibited myeloid, but not erythroid, colony formation. The c-abl sense oligodeoxynucleotides and bcr sense and antisense oligodeoxynucleotides were not inhibitory in this assay. These data show that c-abl is critical in normal myelopoiesis and may explain the relatively selective expansion of leukocytes in patients with chronic myelogenous leukemia.     

Mouse lysozyme production by a monocytoma: isolation and comparison with other lysozymes

A transplantable mouse tumor, GPC-11, produces large amounts of lysozyme. The tumor is a reticulum cell sarcoma, type A, and is a neoplasm of monocytes. The lysozyme was purified from mouse urine in quantities sufficient for structural analysis. Comparison of mouse lysozyme with lysozymes from; chicken egg white and patients with monocytic leukemia reveals similarities in size and electrophoretic mobility and, with human lysozyme, in functional properties; but considerable differences are found in antigenic characteristics and amino acid composition.     

Polymorphism of lactate dehydrogenase in gelada baboons

In a group of 21 gelada baboons, three different lactate dehydrogenase patterns were observed after starch-gel electrophoresis of tissue extracts. The patterns indicate that a mutation has occurred in this population in the gene responsible for the A-subunit. The normal and variant homozygotes each contain five isozymes, those containing the A subunit having characteristically different electrophoretic mobilities. The heterozygote contains the expected 15 isozymes.     

Antigen solubilized from human leukemia: lymphocyte stimulation

Soluble antigen was extracted with hypertonic (3 molar) potassium chloride from the malignant cells of seven patients with acute leukemia. The antigen and leukemia cells were used to stimulate autologous patients' and allogeneic normal donors' lymphocytes in mixed lymphocyte cultures. The lymphocytes of six patients showed significant blastogenic responses to autologous antigen. In contrast, the lymphocytes of only one of seven normal donors responded to the soluble antigens. Both patients' and normal subjects' lymphocytes responded to the intact leukemia cells. The use of these antigens should facilitate the study of specific tumor immunity in human leukemia.     

Externally suppressible proline quadruplet ccc U

Three (+1) frameshift mutations located at different genetic sites respond with high specificity to the same external suppressor. In each case, the suppressor restores small amounts of protein that is normal in electrophoretic mobility and heat stability. One of these proteins has been shown to have the wildtype amino acid sequence. The messenger RNA quadruplet CCCUappears to be common to all three frameshift sites and to be translated by the suppressor as proline. A likely suppressor agent is a proline transfer RNA with a quadruplet anticodon or its functional equivalent.     

RNA metabolism in tracheal epithelium: alteration in hamsters deficient in vitamin A

The electrophoretic pattern of RNA molecules that are synthesized in vitro in tracheal epithelium from hamsters deficient in vitamin A differs from that of RNA synthesized in normal, pair-fed control hamsters. There is less RNA of low electrophoretic mobility in the epithelial cells deficient in vitamin A. This alteration is reversed after the deficient animals have been treated with vitamin A.     

6-phosphogluconate dehydrogenase: hemizygous manifestation in a patient with leukemia

In a study of 41 patients with chronic myelocytic leukemia, two were found to have the 6-phosphogluconate dehydrogenase heterozygous phenotype A-B, and two had the phenotype characteristic of Pd(B) homozygosity. Since one of the two with Pd(B) homozygosity was the mother of two children with the A phenotype, it was presumed that she carried a Pd(A) gene not expressed in her blood cells. his was confirmed by electrophoretic analysis of her fibroblasts, which had the A-B phenotypic pattern. Gene deletion is considered to be the most likely explanation.     

高简并引物小温度范围不规则降落PCR在庞大基因家族扩增中的应用

[目的]探讨高简并性引物扩增庞大基因家族基因的特殊方法。[方法]采用高简并引物对鲤鱼基因组DNA分别进行了常规PCR扩增、常规降落PCR扩增以及优化后的小温度范围不规则跳跃降落PCR扩增。[结果]采用常规PCR仅得1条明显条带,得到的基因较少;采用常规降落PCR仅得到弥散性扩增,无明显条带出现;而采用小温度范围不规则跳跃降落PCR则得到了3条明显条带和多个基因,扩增结果理想。[结论]为庞大基因家族扩增条件的优化与选择提供了理论依据。     

Feline leukemia virus: occurrence of viral antigen in the tissues of cats with lymphosarcoma and other diseases

Felinie leutkemia viruts antigen is demonstrable by immunodiffusion with rabbit precipitating antiserum to purified felinie leukemia virus. The felinie leukemia virus antigen was found in the tissues of 25 of 33 cats with lymphosarcoma and of 5 of 13 cats with infectious peritonitis. Its presence was correlated with the occurrence of felinie leukemia virus demonstrable by electron microscopy. The one clinically normal cat giving a positive test for feline leukemia virus antigen belonged to a household in which two cats had developed lymphosarcoma. With the exception of a dog with lymphosarcoma induced by feline leukemia virus, the antigent was absent from lymphosarcoma and nonlymphomatous tumors of other species (man, dog, cow, goat, or pig).     

Nucleoside phosphatases of fetal and maternal blood cells: electron microscope study

Surface membranes of the blood cells located within the maternal blood sinuses of the rat placenta contain reaction products of nucleoside phosphatases. Fetal blood cells separated from maternal blood by the placental labyrinth show no activity during normal or prolonged gestation. Neonatal blood cells examined 34 hours after parturition show these enzyme activities.     

电泳法与幼苗法鉴定玉米杂交种纯度一致性与相关性分析

以幼苗期具有标记性状的玉米杂交种铁单4号，本育9号和东单54号为试材，利用电泳法和幼苗形态法分别对其纯度进行鉴定后，再对两种方法鉴定结果的一致性和相关性进行统计分析。结果表明；两种方法鉴定的结果无显差异。相关极显，且得到了电泳鉴定纯度（x)与幼苗鉴定纯度（y)回归方程y=7.93 0.9232x。     

Detection of an antigen associated with acute leukemia

Antiserums to a purified cell membrane component from a Burkitt's lymphoma tissue culture cell line were produced in rabbits. These antiserums were cytotoxic to peripheral white blood cells from 8 of 15 patients with acute leukemia and 5 of 41 relatives, but not to peripheral white blood cells from leukemia patients in clinical remission or from normal individuals. These antiserums appear to be detecting an acute leukemia associated antigen or antigens.     

Natural antibodies to human retrovirus HTLV in a cluster of Japanese patients with adult T cell leukemia

Human T cell lymphoma leukemia virus (HTLV) is a human retrovirus (RNA tumor virus) that was originally isolated from a few patients with leukemias or lymphomas involving mature T lymphocytes. Here we report that the serum of Japanese patients with adult T cell leukemia, but not the serum of tested normal donors, contains high titers of antibodies to HTLV. These observations, together with data from Japan showing that adult T cell leukemia is endemic in southwest Japan, suggest that HTLV is involved in a subtype of human T cell malignancy, including Japanese adult T cell leukemia.     

PEST domain-enriched tyrosine phosphatase (PEP) regulation of effector/memory T cells

Protein tyrosine kinases and phosphatases cooperate to regulate normal immune cell function. We examined the role of PEST domain-enriched tyrosine phosphatase (PEP) in regulating T cell antigen-receptor function during thymocyte development and peripheral T cell differentiation. Although normal na?ve T cell functions were retained in pep-deficient mice, effector/memory T cells demonstrated enhanced activation of Lck. In turn, this resulted in increased expansion and function of the effector/memory T cell pool, which was also associated with spontaneous development of germinal centers and elevated serum antibody levels. These results revealed a central role for PEP in negatively regulating specific aspects of T cell development and function.