共查询到19条相似文献,搜索用时 187 毫秒
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为了给桑叶黄酮作为天然抗氧化剂开发提供参考依据,试验以桑叶为研究对象,通过单因素试验分析了超声波辅助、提取时间、提取温度、料液比以及乙醇浓度对桑叶黄酮提取率的影响,利用响应面法优化了桑叶黄酮提取工艺,并结合1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除试验评估了提取物的抗氧化性能。结果表明:超声波辅助能够显著提高桑叶黄酮提取率(P<0.05);响应面模型得到的提取条件为乙醇浓度54.75%、提取时间17.61 min、料液比1:30.24(g/m L)、温度50.27℃,此时桑叶黄酮提取率最高为3.64%;实际测得提取时间17 min、提取温度50℃、料液比1:30(g/m L)、乙醇浓度55%的条件下桑叶黄酮提取率为3.62%,与理论值相对偏差为0.55%,且该条件下提取物DPPH自由基清除活性为99.12%。 相似文献
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蜂胶黄酮提取纯化的工艺研究 总被引:1,自引:0,他引:1
为确定蜂胶黄酮提取纯化的最佳工艺条件.以蜂胶黄酮的得率和绝对提取率为指标,采用正交法优化乙醇提取蜂胶黄酮的工艺条件,考察乙醇浓度、液固比、水浴温度和水浴时间对蜂胶黄酮得率和绝对提取率的影响,再通过大孔吸附树脂对蜂胶黄酮进行纯化.结果表明,最佳提取纯化工艺条件为:乙醇浓度为60%,液固比为18ml/g,水浴温度为55℃,水浴时间为5h,上述工艺条件下,黄酮纯度为72.2%.最优树脂为D101,吸附速度为1.0ml/min,洗脱剂先采用40%乙醇2BV体积洗脱洗去杂质,再用80%乙醇5BV体积洗脱获得黄酮成分,此条件下层析得到的蜂胶黄酮纯度90.2%. 相似文献
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为研究日粮中添加藤茶提取物对不同体重阶段猪生长性能、血液生化指标、抗氧化活性的影响,本研究选用平均初始体重为30 kg的三元杂交(杜×长×大)去势公猪90头,随机分成3个处理,分别为对照组(CON组,饲喂基础日粮)、植物精油复合物组(A组,基础日粮中添加0.03%植物精油复合物)和藤茶提取物组(B组,基础日粮中添加0.03%藤茶提取物),根据不同体重阶段(30~50、50~75、75~100 kg及100 kg~出栏)饲喂不同阶段的基础日粮,每次换料前及试验结束时称重采血,分析不同体重阶段添加0.03%藤茶提取物对猪生长性能、血液生化指标、抗氧化活性的影响。结果表明:与CON组相比,添加0.03%藤茶提取物显著提高了30~75 kg猪的平均日采食量(ADFI)和平均日增重(ADG)(P<0.05);显著降低了30~50 kg猪血清乳酸(LA)含量(P<0.05);显著提高了50~75 kg猪血液胰岛素(INS)浓度(P<0.05),但对血糖(GLU)浓度无显著影响(P>0.05);显著提高了75~100 kg猪GLU浓度(P<0.05),INS浓度有升高趋势(P>0.05),乳酸脱氢酶(LDH)含量显著降低(P<0.05);各体重阶段血清生长激素(GH)含量均有升高趋势(P>0.05)。B组猪血清总超氧化物岐化酶(T-SOD)活力、丙二醛(MDA)含量、谷胱甘肽过氧化物酶(GSH-Px)活力、总抗氧化能力(TAOC)在各体重阶段与CON组相比均无显著差异(P>0.05)。与A组相比,添加0.03%藤茶提取物显著提高了30~100 kg猪的ADFI(P<0.05),显著降低了30~50 kg猪血清LA含量(P<0.05),且显著提高了50 kg以上体重阶段猪血清GSH-Px活力(P<0.05)。综合试验结果,添加0.03%藤茶提取物可促进30~75 kg猪生长代谢,在一定程度改善50~100 kg猪的血糖代谢,且添加藤茶提取物效果优于添加等量植物精油复合物。 相似文献
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SHAN Guang-qiang CHEN Zhi-gang XU Guang-ren LEI Hong-yu TAN Yu-qin SU Jian-ming 《中国畜牧兽医》2016,43(12):3156-3162
To determine the content of total flavonoids from Rosa laevigata Michx. fruits, and optimize the process of cellulase-assisted extraction, the content of total flavonoids from Rosa laevigata Michx. fruits were determined with a double-wavelength spectrophotometry with detection wavelength were 500 nm and 580 nm as the reference, using rutin as a standard material, and the process of cellulase-assisted extraction designed by orthogonal test was optimized with the yield of flavonoids as the evaluation index, and the effects of extraction temperature, concentration of ethanol, cellulase dosage, ratio of liquid to material and other 4 factors on the content of total flavonoids were investigated. It showed a good linearity (r=0.9981) between absorbance difference(ΔA) and the rutin mass ranging of 19.90 to 398.00 μg, the standard curve equation was y=0.0008x-0.0047, and the yield of total flavonoids was 87.94 mg/g, the average recovery and RSD were 98.23% and 8.77%, respectively. And the optimumcellulase-assisted extraction conditions of total flavonoids from Rosa laevigata Michx. fruits by orthogonal array design were extraction temperature of 80℃, concentration of ethanol of 40%, cellulase dosage of 0.5%, ratio of liquid to material of 40:1, extraction time of 90 min, pH of 6.0, and extraction numbers of 2. Various factors affecting extracting yield were in a order of extraction temperature > cellulase dosage > ratio of liquid to material > concentration of ethanol. In this condition of extraction, yield of total flavonoids from Rosa laevigata Michx. fruits was (93.35±3.15) mg/g. Therefore, it was suggested that extraction of total flavonoids from Rosa laevigata Michx. fruits by cellulase-assisted extraction was feasible, and the yield of total flavonoids was higher and its conditions was mild. 相似文献
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为了测定金樱子中的总黄酮含量,并对其纤维素酶辅助提取工艺进行优化,本研究以芦丁为对照品,采用双波长分光光度法,确定测定波长500 nm,参比波长580 nm,对金樱子中的总黄酮含量进行测定;以总黄酮得率为评价指标,考察提取温度、乙醇浓度、纤维素酶用量、液料比等4个因素对总黄酮得率的影响,正交优化纤维素酶辅助提取金樱子总黄酮的工艺。结果表明,在19.90~398.00 μg范围内,芦丁的质量与吸光度差值ΔA呈良好的线性关系(r=0.9981),标准曲线方程为y=0.0008x-0.0047,该方法下测得其得率为87.94 mg/g,平均加样回收率98.23%,相对标准偏差为8.77%;正交试验优化的纤维素酶法辅助提取金樱子总黄酮的最佳工艺条件为:提取温度80℃、乙醇浓度40%、纤维素酶用量0.5%、液料比40:1、提取时间90 min、pH为6.0、提取次数2次,且各因素对金樱子总黄酮提取得率的影响顺序为:提取温度 > 纤维素酶用量 > 液料比 > 乙醇浓度;在该提取条件下,金樱子总黄酮得率为(93.35±3.15)mg/g。因此,纤维素酶法辅助提取金樱子总黄酮可行,得率较高,条件温和。 相似文献
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微波辅助提取苜蓿黄酮方法的研究 总被引:3,自引:1,他引:3
为充分利用紫花苜蓿(Medicago sativa L.)中的黄酮类物质,应用微波辅助提取技术,分别对影响其黄酮提取量的乙醇浓度、微波功率、固液比、提取时间和提取次数进行单因素试验,并在此基础上对各因素的互作效率进行正交试验。结果表明:固液比和乙醇浓度是影响提取量的主要因素,微波功率和其它因素影响较小;微波提取苜蓿黄酮的最佳工艺为:40%乙醇浓度,1:30固液比,微波500 W功率下作用60s;在此工艺条件下,黄酮提取量的理论值为4.92mg/g,接近于实际测定值4.88mg/g。 相似文献
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【目的】 探究牡荆总黄酮的提取工艺,并探讨牡荆提取物对产气荚膜梭菌的抑菌活性。【方法】 以芦丁作为对照品,采用分光光度法对牡荆中的总黄酮含量进行测定;以总黄酮含量为考察指标,先通过单因素试验研究乙醇浓度、提取时间、提取温度、料液比4个单因素对牡荆总黄酮提取量的影响,确定单因素情况下最佳工艺的水平范围;在单因素试验基础上,通过设计L9(34)正交试验,利用NaNO2-AL (NO3)3-NaOH显色法对牡荆总黄酮含量进行测定,绘制标准曲线,计算回归方程,通过精密度、稳定性、重复性、加样回收率进行方法学考察,验证标准曲线。通过将试验所得数据代入标准曲线,比较得出牡荆总黄酮的最佳制备工艺,并在此基础上通过微量二倍稀释法考察其提取物对产气荚膜梭菌的抗菌活性。【结果】 牡荆总黄酮最佳提取工艺条件为:乙醇浓度60%、提取温度50 ℃、提取时间2.5 h、料液比1:35。在此最优提取条件下,牡荆总黄酮含量最高(43.17 mg/g)。其因素影响顺序为料液比>提取时间>乙醇浓度>提取温度,料液比对牡荆总黄酮含量影响最大。最佳工艺下的牡荆提取物对产气荚膜梭菌的最小抑菌浓度(MIC)值为7.81 μg/mL。【结论】 在乙醇浓度60%、提取温度50 ℃、提取时间2.5 h、料液比1:35的条件下提取的牡荆总黄酮含量最高,所制备提取物对产气荚膜梭菌具有较强的抑菌作用,为生产高质量的牡荆提取物提供了理论依据。 相似文献
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The effects of Ampelopsis grossedentata extract on growth performance, diarrhea rate and anti-oxidation function in mice were studied in this experiment. 90 mice were randomly divided into five groups: Blank group, experimental groups Ⅰ,Ⅱ,Ⅲ and control group. The mice in blank group were fed with basal diet, the experimental groups were added with 0.02, 0.04, 0.06 mg/(g·BW) of Ampelopsis grossedentata extract and the control group was added with colistin sulfate in basal diets, respectively. On the 26th day of the trial, the mice were administered folium senna and then record their diarrhea condition. The results showed that, the finial weights of mice in experimental and control groups were higher than that in blank group, and the difference between the blank and control groups were significant (P<0.05). Adding Ampelopsis grossedentata extract had a tendency to increase growth performance and liver weight, but the difference was not significant (P>0.05). The diarrhea rates in the control and experimental groups Ⅱ,Ⅲ were significantly lower than the blank and experimental group Ⅰ(P<0.05). Compared with the blank group, the activity of the serum glutathione peroxidase (GSH-Px) was significantly increased in control group (P<0.05), while the increasing trends were not significant in experimental groups (P>0.05). The activity of catalase (CAT) in control group was significantly higher than that in experimental group Ⅱ (P<0.05), and the serum superoxide dismutase (SOD) activities among the experimental groups were not significant (P>0.05). In conclusions, the growth performance and anti-oxidation function in mice could be increased, while the diarrhea rate could be decreased by adding the Ampelopsis grossedentata extract. It suggested that Ampelopsis grossedentata extract was expected to become a substitute for antibiotics in animal feeds. 相似文献
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试验旨在研究藤茶提取物对小鼠生长性能、腹泻率和抗氧化性能的影响。将90只小鼠随机分为空白组、对照组和试验Ⅰ、Ⅱ、Ⅲ组,空白组小鼠饲喂基础日粮,试验Ⅰ、Ⅱ、Ⅲ组分别在基础日粮中添加0.02、0.04、0.06 mg/g体重的藤茶提取物,对照组在基础日粮中添加0.02 mg/g体重的硫酸抗敌素。在试验第26天给小鼠灌服番泻叶,观察各日粮处理组小鼠腹泻情况。结果显示,各试验组和对照组小鼠试验末体重均高于空白组,且对照组显著高于空白组(P<0.05);添加藤茶提取物有提高小鼠生长性能和肝脏重的趋势,但与空白组相比差异不显著(P>0.05);试验Ⅱ、Ⅲ组和对照组小鼠腹泻率显著低于空白组和试验Ⅰ组(P<0.05);与空白组相比,对照组谷胱甘肽过氧化物酶(GSH-Px)活性显著提高(P<0.05),试验组也有提高趋势,但均未达显著水平(P>0.05);对照组的过氧化氢酶(CAT)活性显著高于试验Ⅱ组(P<0.05),各试验组之间超氧化物歧化酶(SOD)活性无显著差异(P>0.05)。以上结果表明,日粮中添加藤茶提取物能够能提高小鼠的生长性能及抗氧化性能,降低小鼠的腹泻率,可作为抗生素的替代品进一步研发。 相似文献
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