Effects of iron-ore mining and processing on metal bioavailability in a tropical coastal lagoon

Background, aim, and scope

In water systems, water quality and geochemical properties of sediments determine the speciation of trace metals, metal transport, and sediment–water exchange, influencing metal availability and its potential effects on biota. Studies from temperate climates have shown that iron-ore mining and tailing wastewaters, besides being a source of trace metals, usually show high levels of dissolved ions and particulate suspended matter, thus having the potential of indirectly changing metal bioavailability. For the first time in the tropics, we identified the effects of iron-ore mining and processing on metal bioavailability in a coastal lagoon. With an extensive sampling scheme, we investigated the potential sources of metals; the links among metal levels in water, sediments, and invertebrates; and the contrasting effects on metal speciation and bioavailability.

Methodology

The metals Fe, Mn, Al, Cr, Zn, Cu, Ni, Pb, Cd, Hg, and As were measured in water, sediments (surface and profiles), and invertebrates from Mãe-Bá Lagoon and in the sites directly influenced by the mining operations (tailing dams and nearby rivers). In addition, samples from two other lagoons, considered pristine, were analyzed. The study area is located in the southeast of Brazil (Iron Quadrangle Region and a coastal area of Espírito Santo State). General water characteristics included pH, dissolved organic carbon, alkalinity, and anion composition. Water metal speciation was assessed by a speciation model (Chemical Equilibria in Aquatic Systems). Grain-size distribution, organic carbon, carbonate, and acid volatile sulfide (AVS) were determined in sediments. Statistical methods included comparison of means by Mann–Whitney test, ordination and correlation analyses, and analysis of regression for geochemical normalization of metals with grain size.

Results and discussion

The dissolved metal concentrations, the total metal levels in sediments, and the normalization based on the fine sediment fraction showed that the mining operations constitute potential sources of Fe, Mn, Cr, Cu, Ni, Pb, As, and Hg to Mãe-Bá Lagoon. However, trace metal availability was reduced because of increased pH, hardness, and sulfide content (356 μmol/g) in the sites influenced by the mining. The lagoon showed similar water chemistry as in the mining sites, with metal bioavailability further decreased by the presence of dissolved organic carbon and chloride. Although AVS levels in the lagoon were low (0.48–56 μmol/g), metal bioavailability was reduced because of the presence of organic matter. Metal levels in invertebrates confirmed the predicted low metal bioavailability in Mãe-Bá Lagoon. The lagoon was considered moderately contaminated only by Hg and As.

Conclusions

The iron-ore mining and processing studied here constitute potential sources of metal pollution into the tropical lagoon. Contrary to expectations, however, it also contributes to reducing the overall metal bioavailability in the lagoon.

Recommendations and perspectives

These findings are believed to be useful for evaluating metal exposure in a more integrated way, identifying not only the sources of pollution but also how they can affect the components involved in metal speciation and bioavailability in water systems, leading to new insights.     

Temporal variation in methanogenic community structure and methane production potential of tropical rice ecosystem

Temporal variations in diversity of methanogenic community and CH₄ production potential were analyzed in an Indian tropical rice ecosystem. Laboratory incubations showed that methane production varied from 20.86 to 134.11 μg CH₄ g⁻¹ d.w.s. during the two consecutive years, 2009 and 2010. CH₄ production potential was high at the flowering stage of the rice crop followed by ripening, tillering, post-harvest and pre-plantation stage. Phylogenetic analysis of 16S rRNA genes of methanogenic community indicated that flowering and ripening stages comprised of Methanomicrobiaceae, Methanosarcinaceae, Methanosaetaceae and RC I methanogenic groups, while only the members of Methanomicrobiaceae and RC I were present in the remaining stages. Further, the dominance of RC I was observed in all stages. This study demonstrates that flowering and ripening stages of rice crop offer relatively favorable ecological niche for methanogenic community. The overall analyses suggest that the temporal change in diversity of methanogens regulates CH₄ production potential in rice field soils.     

Phosphate-solubilizing microorganisms associated with the rhizosphere of mangroves in a semiarid coastal lagoon

 The phosphate-solubilizing potential of the rhizosphere microbial community in mangroves was demonstrated when culture media supplemented with insoluble, tribasic calcium phosphate, and incubated with roots of black (Avicennia germinans L.) and white [Laguncularia racemosa (L.) Gaertn.] mangrove became transparent after a few days of incubation. Thirteen phosphate-solubilizing bacterial strains were isolated from the rhizosphere of both species of mangroves: Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus atrophaeus, Paenibacillus macerans, Vibrio proteolyticus, Xanthobacter agilis, Enterobacter aerogenes, Enterobacter taylorae, Enterobacter asburiae, Kluyvera cryocrescens, Pseudomonas stutzeri, and Chryseomonas luteola. One bacterial isolate could not be identified. The rhizosphere of black mangroves also yielded the fungus Aspergillus niger. The phosphate-solubilizing activity of the isolates was first qualitatively evaluated by the formation of halos (clear zones) around the colonies growing on solid medium containing tribasic calcium phosphate as a sole phosphorus source. Spectrophotometric quantification of phosphate solubilization showed that all bacterial species and A. niger solubilized insoluble phosphate well in a liquid medium, and that V. proteolyticus was the most active solubilizing species among the bacteria. Gas chromatographic analyses of cell-free spent culture medium from the various bacteria demonstrated the presence of 11 identified, and several unidentified, volatile and nonvolatile organic acids. Those most commonly produced by different species were lactic, succinic, isovaleric, isobutyric, and acetic acids. Most of the bacterial species produced more than one organic acid whereas A. niger produced only succinic acid. We propose the production of organic acids by these mangrove rhizosphere microorganisms as a possible mechanism involved in the solubilization of insoluble calcium phosphate. Received: 21 April 1999     

Depression of methane production potential in paddy soils by subsurface drainage systems

Abstract

Subsurface drainage systems (pipe/tile drain systems) in paddy fields have been used in Japan since the 1960s for appropriate water management to encourage rice growing. Water management using the drainage systems probably accelerates the aerobic decomposition of organic matter in the paddy soils, and the management using the systems also accelerates leaching of water-soluble fractions in the soils. To evaluate these side-effects of the drainage systems on methane (CH₄) production potential in the soils, soil samples taken from four pairs of paddy fields with or without drainage systems (D-soils and ND-soils, respectively) were compared. In general, total C and N, hot-water-extractable hexose, ammonification and Fe²⁺ production were lower in D-soils than in ND-soils. Decomposition of buried rice straw during a fallow period was also accelerated in D-soils. Hence, both electron acceptors, such as reducible Fe, and electron donors, such as easily decomposable organic matter, in D-soils decreased on a short-term and long-term basis. To compare the effect of decreased electron acceptors and donors on the same criterion (mg C_eq kg^?1 dry matter (d.m.)), the oxidative capacity (OxiC) and reductive capacity (RedC) in each soil were calculated from the soil chemical and biological properties. Both OxiC and RedC decreased in D-soils, but the rate of decrease in RedC was 2.7-fold higher than that of OxiC. As the soil conditions became relatively oxidative, CH₄ production potential in D-soils decreased by approximately 40%. Thus, the installation of subsurface drainage systems under poorly drained paddy fields relatively decreased RedC in soil, and that CH₄ production potential in the soil also decreased.     

Methane production potential and methanogenic archaeal community structure in tropical irrigated Indian paddy soils

Soil characteristics regulate various belowground microbial processes including methanogenesis and, consequently, affect the structure and function of methanogenic archaeal communities due to change in soil type which in turn influences the CH₄ production potential of soils. Thus, five different soil orders (Alfisol, Entisol, Inceptisol, Podzol and Vertisol) were studied to assess their CH₄ production potential and also the methanogenic archaeal community structure in dryland irrigated Indian paddy soils. Soil incubation experiments revealed CH₄ production to range from 178.4 to 431.2 μg CH₄ g^-1 dws in all soil orders as: Vertisol<Inceptisol<Entisol<Podzol<Alfisol. The numbers of methanogens as quantified using real-time quantitative polymerase chain reaction (qPCR) targeting mcrA genes varied between 0.06 and 72.97 (×10⁶ copies g^-1 dws) and were the highest in Vertisol soil and the least in Alfisol soil. PCR-denaturing gradient gel electrophoresis (DGGE)-based approach targeting 16S rRNA genes revealed diverse methanogenic archaeal communities across all soils. A total of 43 DGGE bands sequenced showed the closely related groups to Methanomicrobiaceae, Methanobacteriaceae, Methanocellales, Methanosarcinaceae, Methanosaetaceae and Crenarchaeota. The composition of methanogenic groups differed among all soils and only the Methanocellales group was common and dominant in all types of soils. The highest diversity of methanogens was found in Inceptisol and Vertisol soils. Methane production potential varied significantly in different soil orders with a positive relationship (p?<?0.05) with methanogens population size, permanganate oxidizable C (POXC) and CO₂ production. The present study suggested that CH₄ production potential of different soils depends on physicochemical properties, methanogenic archaeal community composition and the population size.     

Inhibitors of methane production in paddy soils

Global warming is now attracting the world attention. Methane is an important greenhouse gas next to CO₂. Prather et al. (1995) estimated that rice paddy fields account for 14% of all biogenic atmospheric methane. It is considered that methane production from rice paddy fields is increasing along with the increase of the population. Therefore, the development of rice cultivation techniques for reducing methane production is essential, in order to preserve the global environment.     

Carbon sinks in mangroves and their implications to carbon budget of tropical coastal ecosystems

Nearly 50% of terrigenous materials delivered to the world's oceans are delivered through just twenty-one major river systems. These river-dominated coastal margins (including estuarine and shelf ecosystems) are thus important both to the regional enhancement of productivity and to the global flux of C that is observed in land-margin ecosystems. The tropical regions of the biosphere are the most biogeochemically active coastal regions and represent potentially important sinks of C in the biosphere. Rates of net primary productivity and biomass accumulation depend on a combination of global factors such as latitude and local factors such as hydrology. The global storage of C in mangrove biomass is estimated at 4.03 Pg C; and 70% of this C occurs in coastal margins from 0° to 10° latitude. The average rate of wood production is 12.08 Mg ha^?1 yr^?1, which is equivalent to a global estimate of 0.16 Pg C/yr stored in mangrove biomass. Together with carbon accumulation in mangrove sediments (0.02 Pg C/yr), the net ecosystem production in mangroves is about 0.18 Pg C/yr. Global estimates of export from coastal wetlands is about 0.08 Pg C/yr compared to input of 0.36 Pg C/yr from rivers to coastal ecosystems. Total allochthonous input of 0.44 Pg C/yr is lower than in situ production of 6.65 Pg C/yr. The trophic condition of coastal ecosystems depends on the fate of this total supply of 7.09 Pg C/yr as either contributing to system respiration, or becoming permanently stored in sediments. Accumulation of carbon in coastal sediments is only 0.41 Pg C/yr; about 6% of the total input. The NEP of coastal wetlands also contribute to the C sink of coastal margins, but the source of this C is part of the terrestrial C exchange with the atmosphere. Accumulation of C in wood and sediments of coastal wetlands is 0.205 Pg C/yr, half the estimate for sequestering of C in coastal sediments. Burial of C in shelf sediments is probably underestimated, particularly in tropical river-dominated coastal margins. Better estimates of these two C sinks in the tropics, coastal wetlands and shelf sediments, is needed to better understand the contribution of coastal ecosystems to the global carbon budget.     

Fluxes of methane from rice fields and potential for mitigation

Abstract. Methane (CH₄) is an important greenhouse gas. Flooded rice fields (paddies) are a significant source of atmospheric CH₄; estimates of the annual emission from paddies range from less than 20 to 100 million Tg, with best estimates of 50 × 20 Tg. The emission is the net result of opposing bacterial processes: production in anaerobic microenvironments, and consumption and oxidation in aerobic microenvironments, both of which occur sequentially and concurrently in flooded rice soils. With current technologies, CH₄ emission from rice fields will increase as production increases. Over the next 25 years rice production will have to increase by 65% from the present 460 Mt/y to 760 Mt/y in 2020. The current understanding of the processes controlling CH₄ fluxes, rice growth and rice production is sufficient to develop mitigation technologies. Promising candidates are changes in water management, rice cultivars, fertilization, and cultural practices. A significant reduction of CH₄ emission from rice fields, at the same time that rice production and productivity increase at the farm level, is feasible, although the regions where particular practices can be applied, and the trade-offs that are possible, have still to be identified.     

Nitrification in forest soil of different pH as affected by urea,ammonium sulphate and potassium sulphate

Nitrification was inhibited by ammonium sulphate and potassium sulphate added to soil from the organic horizon (pH 4.7) of a Myrtillus-type pine forest. Urea did not inhibit nitrification. Soil pH was slightly decreased by the salts but increased by urea. The salts increased soil electrical conductivity more than urea did. The inhibition of nitrification following salt treatments was probably due to a decrease in soil pH and not to osmotic effects. In acid conditions, the salts had a less inhibitory effect on CO₂ production than on nitrification, indicating that nitrifying bacteria were more sensitive than other organisms to the salts.     

Importance of rice root oxidation potential as a regulator of CH4 production under waterlogged conditions

Biology and Fertility of Soils - One of the most important characteristics of a rice cultivar controlling methane (CH4) production can be the root oxidation potential because a cultivar with a high...     

Influence of the insecticide carbofuran on the production and oxidation of methane in a flooded rice soil

Applications of a commercial formulation of carbofuran, a carbamate insecticide, at rates of 2kg and 12kg active ingredient ha^–1 to flooded fields planted to rice led to significant inhibition of methane emission. Likewise, laboratory incubation studies showed that carbofuran applied at low rates (5 and 10μgg^–1soil) inhibited the net methane production relative to that of the control, but stimulated it when applied at a rate of 100μgg^–1soil. Interestingly, carbofuran increased the oxidation of methane when applied at low rates and inhibited it when applied at a rate of 100μgg^–1soil. Received: 5 May 1997     

Small-scale heterogeneity in carbon dioxide, nitrous oxide and methane production from aggregates of a cultivated sandy-loam soil

Spatial variability in carbon dioxide (CO₂), nitrous oxide (N₂O) and methane (CH₄) emissions from soil is related to the distribution of microsites where these gases are produced. Porous soil aggregates may possess aerobic and anaerobic microsites, depending on the water content of pores. The purpose of this study was to determine how production of CO₂, N₂O and CH₄ was affected by aggregate size and soil water content. An air-dry sandy loam soil was sieved to generate three aggregate fractions (<0.25 mm, 0.25–2 mm and 2–6 mm) and bulk soil (<2 mm). Aggregate fractions and bulk soil were moistened (60% water-filled pore space, WFPS) and pre-incubated to restore microbial activity, then gradually dried or moistened to 20%, 40%, 60% or 80% WFPS and incubated at 25 °C for 48 h. Soil respiration peaked at 40% WFPS, presumably because this was the optimum level for heterotrophic microorganisms, and at 80% WFPS, which corresponded to the peak N₂O production. More CO₂ was produced by microaggregates (<0.25 mm) than macroaggregate (>0.25 mm) fractions. Incubation of aggregate fractions and soil at 80% WFPS with acetylene (10 Pa and 10 kPa) and without acetylene showed that denitrification was responsible for 95% of N₂O production from microaggregates, while nitrification accounted for 97–99% of the N₂O produced by macroaggregates and bulk soil. This suggests that oxygen (O₂) diffusion into and around microaggregates was constrained, whereas macroaggregates remained aerobic at 80% WFPS. Methane consumption and production were measured in aggregates, reaching 1.1–6.4 ng CH₄–C kg⁻¹ soil h⁻¹ as aggregate fractions and soil became wetter. For the sandy-loam soil studied, we conclude that nitrification in aerobic microsites contributed importantly to total N₂O production, even when the soil water content permitted denitrification and CH₄ production in anaerobic microsites. The relevance of these findings to microbial processes controlling N₂O production at the field scale remains to be confirmed.     

Contribution of humic substances as a sink and source of carbon in tropical floodplain lagoons

Purpose

We evaluated the decay of humic (HA) and fulvic acids (FA) in order to discuss the contribution of these substances as a sink and source of carbon in a tropical lagoon.

Materials and methods

Experiments were conducted under aerobic and anaerobic conditions using FA and HA isolated from decomposition of Oxycaryum cubense submitted to 10 and 60 days of degradation. HA and FA were added to water samples from a tropical floodplain oxbow system, the Infernão Lagoon. The mineralization chambers were incubated in the dark at 21.0 °C. The carbon balance, electrical conductivity, pH, and optical density were measured over 95 days.

Results and discussion

The results from the carbon budget were fitted with a first-order kinetics model. The mineralization of refractory fractions predominated for both FA and HA. Overall, although the mineralization pathway yields varied according to the type of resource and oxygen availability, the mineralization half-lives were quite similar (49 to 64 days), suggesting a similar microbial catabolism efficiency during the decay of humic substances. The short-term routes are represented by biochemical oxidations, and the immobilization and labile fractions (varying from 0 to 30%) of FA and HA supported these processes. A yield varying from 61.0 to 91.3% represents a carbon source degradation in the middle term (ca. 2 months) considering the ecosystem.

Conclusions

In tropical floodplain lagoons, there are three carbon routes: (i) the IN₁, representing a short-term pathway (hours to days) in the carbon transformation and (ii) IN₃, a middle-term carbon source from HA and FA mineralization to the water column and subsequently to the atmosphere. A third route (IN₂) supported the heterotrophic metabolism of the lagoon acting as a transitory sink of carbon.

     

Impact of coastal wetland cultivation on microbial biomass, ammonia-oxidizing bacteria, gross N transformation and N2O and NO potential production

A ¹⁵N dilution experiment was carried out to investigate effects of cultivation on the gross N transformation rate in coastal wetland zone. Microbial community composition was estimated by phospholipid fatty acid (PLFA) analysis and abundance of soil ammonia-oxidizing bacteria (AOB) was quantified by real-time polymerase chain reaction (PCR). Soil salinity decreased significantly, while total N increased after coastal wetland was cultivated. Microbial biomass (total PLFA), bacterial biomass, fungal biomass, and actinomycete biomass of the native coastal wetland soils were significantly (p < 0.05) lower than those of the cultivated soils whereas AOB population size also significantly increased after coastal wetland cultivation. Multiple regression analysis showed that total PLFA biomass and soil total N (TN) explained 97% of the variation of gross N mineralization rate in the studied soils (gross mineralization rate = 0.179 total PLFA biomass + 5.828TN − 2.505, n = 16, p < 0.01). Gross nitrification rate increased by increasing the soil AOB population size and gross mineralization rate (M) (gross nitrification rate = 3.39AOB + 0.18 M − 0.075, R ² = 0.98, n = 16, p < 0.01). Management of salt discharge and mineral N fertilization during the cultivation of wetland soils might have changed composition of soil microflora and AOB population size, thus influencing mineralization and nitrification. Probably, the cultivation of coastal wetland soils increased the risk of N losses from soil through nitrate leaching and gas emission (e.g., N₂O and NO).     

Enzyme activities as affected by soil properties and land use in a tropical watershed

Enzyme activities play key roles in the biochemical functioning of soils, including soil organic matter formation and degradation, nutrient cycling, and decomposition of xenobiotics. Knowledge of enzyme activities can be used to describe changes in soil quality due to land use management and for understanding soil ecosystem functioning. In this study, we report the activities of the glycosidases (β-glucosidase, α-galactosidase, and β-glucosaminidase), acid phosphatase, and arylsulfatase, involved in C (C and N for β-glucosaminidase), P, and S cycling, respectively, as affected by soil order and land use within a watershed in north-central Puerto Rico (Caribbean). Representative surface soil (0–15 cm) samples were taken from 84.6% of the total land area (45,067 ha) of the watershed using a completely randomized design. The activity of α-galactosidase was greater in soils classified as Oxisols than in soils classified as Ultisols and Inceptisols, and it was not affected by land use. The activity of β-glucosidase was greater in Oxisols compared to the Inceptisols and Ultisols, and it showed this response according to land use: pasture > forest > agriculture. The activity of β-glucosaminidase was higher in Oxisols than the other soil orders, and it was higher under pasture compared to forest and agriculture. Acid phosphatase and arylsulfatase activities were greater in Oxisols and Ultisols than in Inceptisols, and they decreased in this order due to land use: forest = pasture > agriculture. As a group, β-glucosaminidase, β-glucosidase, and acid phosphatase activities separated the sites under forest and pasture from those under agriculture in a three-dimensional plot. Thus, enzyme activities in Inceptisols under agriculture could be increased to levels comparable to other soil orders with conservative practices similar to those under pasture and secondary forest growth. Our findings demonstrate that within this watershed, acid and low fertility soils such as Oxisols and Ultisols have in general higher enzyme activities than less weathered tropical soils of the order Inceptisols, probably due to their higher organic matter content and finer texture; and that the activities of these enzymes respond to management with agricultural practices decreasing key soil biochemical reactions of soil functioning.     

Effects of vegetation regime on denitrification potential in two tropical volcanic soils

Effects of vegetation and nutrient availability on potentail denitrification rates were studied in two volcanic, alluvial-terrace soils in lowland Costa Rica that differ greatly in weathering stage and thus in availability of P and base cations. Potential denitrification rates were significantly higher in plots where vegetation had been left undisturbed than in plots where all vegetation had been removed continuously, and were higher on the less fertile of the two soils. The potential denitrification rates were correlated strongly with respiration rates, levels of mineralizable N, microbial biomass, and moisture content, and moderately well with concentrations of extractable NH _inf4 ^sup+ , Kjeldahl N, and total C. In all plots, denitrification rates were stimulated by the removal of O₂ and by the addition of glucose but not by the addition of water or NO _inf3 ^sup- .This is Paper 2772 of the Forest Research Laboratory, Oregon State University     

Saccharidic compounds as soil redox effectors and their influence on potential N2O production

Cellulose, xylan, and glucose were compared in waterlogged soil as modifying factors of the redox potential (Eh), of the quantity of reducing equivalents, and of the soil capacity to produce N₂O and CO₂. During the study period (168 h) soils supplied with glucose and xylan showed a higher Eh decrease than the control soil and the soil treated with cellulose. In samples taken after 0, 24, 48, and 168 h, the soils supplied with C showed a higher number of reducing equivalents than the control soil did. These quantities were not correlated with Eh values, nor with N₂O production. N₂O production was increased compared with the control soil over the entire experimental period in the glucose-amended soils but only after 48 h in the xylan-amended soils and not until 168 h in the cellulose-treated soils. The CO₂:N₂O ratio was consistently higher than the theoretical value of 2, suggesting that denitrification and CO₂ production via fermentation occurred simultaneously. Moreover, this ratio was highly correlated with the Eh values. We conclude that more research is needed to explain the role of soil redox intensity (Eh) and capacity (quantity of redox species undergoing reduction) in the expression of soil denitrification-fermentation pathways.     

Nitrogen fixation as influenced by moisture content,ammonium sulphate and organic sources in a paddy soil

The effect of moisture and (NH₄)₂SO₄ on N₂ fixation in a paddy soil was investigated employing C₂H₂ reduction assay and ¹⁵N-tracers. N₂ fixation was negligible under nonflooded conditions. Soil submergence accelerated N₂ fixation; with a further increase in N₂ fixation when the flooded soil was incubated under an Ar atmosphere. Rice straw additions to both moist and flooded soils enhanced N₂ fixation. N₂-ase activity in the soil decreased with increasing concentration of added N although complete suppression of the activity was not evident even at concentrations as high as 160–320 parts/10⁶ N. A similar trend of inhibition by N was also noticed in soils amended with glucose or cellulose in combination with N. However, the inhibitory effect of N decreased with increased incubation of soil except at 320 parts/10⁶ N.     

Diversity and antibiotic-producing potential of cultivable marine-derived actinomycetes from coastal sediments of Turkey

Purpose

Marine environments, especially sediments, are rich sources of actinomycetes that provide many bioactive compounds, primarily antibiotics. The goal of this study was to investigate the diversity of cultivable actinomycetes and their potential to produce antibiotics from sediments collected from the coastal zones of Turkey.

Materials and methods

Thirty sediment samples were collected from nine different coastal sites in three seas surrounding the Anatolian Peninsula of Turkey. Of the samples, 6 were collected from one site in the Black Sea, 18 from seven sites in the Aegean Sea, and 6 from one site in the Mediterranean Sea. Strains of pure actinomycetes were isolated by modified actinomycetes isolation agar (MAIA), M1 agar, M6 agar, and modified R2A agar. Ethyl acetate extracts and fermentation broths were used for the evaluation of antimicrobial activity against antibiotic resistant test microorganisms. The identification of the isolates was undertaken by 16S rRNA gene sequencing.

Results and discussion

A total of 261 strains of actinomycetes were isolated, of which 66 (25 %) were active against at least one antibiotic-resistant microorganism. Sixty-five of the actinomycetes isolates with antimicrobial activity were Streptomyces spp. and one was Nocardia sp., which implied that genus Streptomyces was predominant. Whereas MAIA agar was the best medium to recover actinomycetes, M6 agar was superior to others for the isolation of antibiotic-producing strains.

Conclusions

Extensive screening of the extracts from the 261 isolates for antimicrobial activities revealed considerable potential to produce antibiotics. These findings imply that actinomycetes from marine sediments of the Anatolian Peninsula coasts have potential for the discovery of novel bioactive compounds.