Managing resistance to anticoagulant rodenticides: An appraisal

The action necessary for resistance management is specified and compared with what has actually been done, with reference to executive, extension and research activities and to the role of rodenticides and rodenticide development. Some obstacles to resistance management are discussed. To account for the fact that no plausible programme to manage resistance has been developed over the last 36 years, the hypothesis is examined that resistance management is injurious to the parties concerned and, hence, that resistance is perceived not as a problem but as an exploitable asset. If correct, this hypothesis yields the prediction that the action necessary for resistance management will be taken when such action is judged to be economically efficient. The establishment of the Rodenticide Resistance Action Committee indicates that this time may be approaching.     

The influence of anticoagulant resistance on effective rodent control in the UK1

Widespread use of warfarin, and other multiple-dose anticoagulants, selected populations of warfarin-resistant rodents in the UK and in other countries. The effectiveness of rodent control was dramatically reduced when the resistant animals formed a large proportion of a population. The second-generation anticoagulants were developed to overcome these practical control problems with warfarin and were considered to be effective rodenticides against warfarin-resistant populations. In the UK, however, difenacoum showed reduced efficacy for controlling an established warfarin-resistant Rattus norvegicus population covering a substantial area of farmland in southern England. Continued use of difenacoum selected for difenacoum-resistant animals, so that the rodenticide is now ineffective for controlling rats in three counties. Brodifacoum was subsequently used to control some infestations and there is evidence that these treatments selected for animals with an increased level of resistance to brodifacoum. Anticoagulant resistance in Mus domesticus is less of a practical problem because of the availability of non-anticoagulant rodenticides for the control of this species. There is evidence, however, of bromadiolone resistance in M. domesticus trapped on farms in the UK. Recent investigations of the mechanism of anticoagulant resistance in R. norvegicus have indicated that vitamin K3 (menadione) is an antidote to anticoagulants in resistant animals. This form of the vitamin is included as a dietary supplement in poultry and pig food and would aid the survival, and therefore increase the selection, of resistant animals when anticoagulants are used exclusively.     

2种不育剂和1种抗凝血剂对藏北草原高原鼠兔的控制效果

2012年4月至2013年4月在藏北草原进行了炔雌醚、莪术醇和增效敌鼠钠盐控鼠试验。均配制成青稞药饵,投放药饵后第20、75和350天进行控效调查。结果显示:炔雌醚具杀灭高原鼠兔作用,20d校正灭效达72.24%,对成年雌雄鼠繁殖器官均能起到明显损坏作用,且适口性最好;增效敌鼠钠盐控鼠效果最好,20d校正灭效达94.46%;莪术醇也有一定效果,75d幼体比最低,总体看,3种药物均具有一定控制效果,有一定实际应用潜力,但还需进一步试验验证其控鼠效果。     

蔗扁蛾在广东省的危害范围调查

经２年多的调查,蔗扁蛾在广东危害的植物达９科２８种。其中危害最严重的是巴西木这１０－８３％,其次是发财树高达５０％。同时在香蕉上也发现有危害。     

Performance studies with the new anticoagulant rodenticide,flocoumafen, against Mus domesticus and Rattus norvegicus1

Flocoumafen is a new, highly potent anticoagulant rodenticide from Shell Research. It is effective against all the economically important species tested to date, including those with warfarin-resistant strains. This paper is concerned with its performance against two important commensal rodent species, Mus domesticus and Rattus norvegicus. In laboratory studies these two pest species may absorb a lethal dose of flocoumafen by taking a 0.005% (50 ppm) bait as part of their food intake on only one occasion. This single-feed potency of flocoumafen, combined with its good intrinsic palatability, makes it ideal for use in pulse baiting. Marketed under the Shell trade mark Storm, the principal bait formulation selected for commercialization is the wax-bound block. Recent advances in formulation development include increased resistance to attack by insects and fungi and the incorporation of a bitter taste which acts as a deterrent for humans without influencing the palatability of the bait to rodents. Flocoumafen's outstanding potency has been confirmed in a wide range of field trials. In the UK alone, almost 70 pen and field trials have been successfully completed with a mean estimated control in excess of 95%.     

土壤不同处理对木霉菌定殖及其生防效果的影响

重茬土经施肥改土、高温灭菌后,增施木霉菌的试验结果表明:木霉菌可以在甜瓜根表、根际定殖存活,同时不影响其他有益微生物的正常生长;土壤经高温灭菌后,土壤中的微生物呈真空状态,有利于木霉菌的快速定殖,促进甜瓜生长,降低甜瓜枯萎病的病情指数。甜瓜枯萎病发病率与土壤中镰刀菌孢子的存活量成正相关,与土壤中木霉菌、细菌数量的多少成负相关。     

不同灌水参数组合时田面坡度对灌水质量的影响研究

田面坡度是影响畦灌灌水质量的重要因素,研究田面坡度对灌水性能的影响时,要结合灌水定额、平整精度、田面糙率和单宽流量等参数进行分析.通过对常规平地技术条件下多组合灌溉参数模拟分析得出:不同灌水定额、平整精度、田面糙率和单宽流量组合对应不同的最佳田面坡度,当灌水定额为60.00 mm,平整精度为4.25 cm,田面糙率为0...     

An investigation of genetic variation in Cirsium arvense field patches

The genetic structure of typical Cirsium arvense patch populations in two arable fields was examined. Patches were mapped and plant samples were taken in these patches. Plants of a central patch and four surrounding patches were sampled in 1 year to investigate the influence of root fragment dispersal. Plants of another patch were sampled in three subsequent years to investigate the patch development. The genotypes of the plants were examined using repetitive enterogenic primer (REP) and inter simple sequence repeat (ISSR) analysis. The mean proportion of distinguishable genotypes ranged from 0.13 to 0.67 and the evenness index ranged from 0.58 to 1.00. Differences in genotypes between neighbouring patches indicated that root fragment dispersal via soil cultivation was of minor importance. Three years sampling within a patch showed that the patch mainly ‘grew’ via the establishment of new clones rather than by clonal growth of one genotype. The influence of various factors caused by population demography or arable practice on patch development in an arable field is discussed. Although various factors can help to maintain genetic diversity, there is strong evidence for regular seedling establishment in arable fields.     

土壤处理防治柑橘立枯病的效果及对枳橙的影响

对土壤进行药剂消毒后播种枳橙,以研究杀菌剂处理土壤防治立枯病的效果以及对枳橙的影响。结果表明,40%甲醛、丙森锌、咪鲜胺、噁霉灵、代森锰锌、甲基硫菌灵、硫酸亚铁等7种药剂表现出较好的防病效果。7种杀菌剂处理土壤后,枳橙的发芽率较高,砧木苗的成苗率、成苗数、茎粗、地上部高度及根长均显著优于对照。多菌灵防病效果较差。     

尖孢镰刀菌侵染对马铃薯光合效率和叶绿素荧光参数影响

为研究枯萎病对马铃薯光合特性的影响,在幼苗期接种尖孢镰刀菌Fusarium oxysporum后,统计抗病品种陇薯10号和感病品种新大坪的病情指数,并测定叶绿素含量、光合及荧光参数。结果表明,尖孢镰刀菌侵染30 d后马铃薯表现出枯萎病症状,叶绿素含量显著降低,其中叶绿素a含量降幅最大,抗病品种陇薯10号比对照降低9.64%,感病品种新大坪比对照降低14.24%。尖孢镰刀菌侵染后马铃薯光合效率显著降低,侵染30 d后,抗病品种陇薯10号净光合速率比对照降低39.56%,感病品种新大坪比对照降低47.13%。病株的光响应曲线参数光补偿点、暗呼吸速率和表观量子效率都显著提高;而光饱和点和最大净光合速率都显著低于对照,表明尖孢镰刀菌侵染缩小了马铃薯对光能的利用有效范围。病株CO2响应曲线参数CO2饱和点、最大净光合速率和羧化效率分别显著低于对照;病株CO2补偿点和光呼吸速率反而升高,说明碳同化过程受到尖孢镰刀菌的限制。暗适应下的初始荧光、最小荧光、最大荧光、PSII最大光化学效率、光适应下PSII最大光化学效率及实际光化学效率、光化学猝灭系数、非光化学猝灭系数和光合电子传递速率均显著低于对照,...     

Exploring drivers of the usage of anticoagulant rodenticides on UK farms

Anticoagulant rodenticides (ARs) are considered inhumane, show increasingly limited efficacy due to acquired resistance, and carry environmental consequences associated with non-target species uptake. In a questionnaire study of 499 UK farms that all deployed chemical rodenticide we found a high mean reliance (79%), on second generation ARs with just over half of the respondents using no other rodent control methods. Additional methods where deployed, alone or in combination, included predation (41%), kill-trap deployment (16%) and shooting (1%). Nearly 40% of all respondents deployed rodenticides year-round. There was no evidence to suggest that “tidy-farm” measures, such as clearing food spills and minimising on-farm rodent harbourage sites aimed at minimising rodent-associated problems, were associated with a lower likelihood of year-round deployment; in fact trends in our analyses suggested the opposite. We therefore encourage operators to fully evaluate the true necessity of rodenticide deployment before AR use.     

An investigation of the non-solvent-extractable residues of [14C]chlorpyrifos-methyl in stored wheat

The residues of [¹⁴C] chlorpyrifos-methyl remaining in wheat after solvent extraction accounted for 28% of the applied dose after an extended period of storage. A bioassay of these residues was carried out using Tribolium castaneum and a significant reduction in the numbers of adults reaching maturity was observed. Attempts to release the non-solvent-extractable residues using an enzymic digestion procedure were unsuccessful, as were several of the chemical solubilisation procedures investigated. A water/methanol extraction system was successful in releasing up to 86% of the radioactivity leaving 10% still non-extractable. Analysis of this solubilised residue revealed that 59% was present as the pyridinol metabolite of chlorpyrifos-methyl and 26% was a polar material composed of two main components. Bioassay tests of these metabolites revealed that the reduction in the number of insects reaching maturity could be associated with the pyridinol metabolite.     

An investigation of herbicide interaction with the H+-ATPase activity of plant plasma membranes

This study has investigated the activity of several herbicide classes at the plant plasma membrane. Two-phase partitioning was used to prepare highly purified plasma membrane vesicles from the monocotyledon weed black-grass (Alopecurus myosuroides Huds.) and the dicotyledon crop sugar beet (Beta vulgaris L. cv. Celt). The purity of the plasma membrane H⁺-ATPase activity was characterised with respect to inhibitors, pH and substrate specificity. In both species, contamination of the plasma membrane by tonoplast fragments was largely eliminated and chlorophyll was absent. In addition, the plasma membrane H⁺-ATPase from black-grass and sugar beet exhibited high vanadate sensitivity and a sharp pH profile around 6·5. Subsequently, H⁺-ATPase activity was assayed in the presence (100 μM ) and absence of four graminicide classes and auxin-type herbicides. Graminicides, including the aryloxyphenoxypropionate diclofop-methyl and the thiocarbamate triallate, inhibited H⁺-ATPase activity by 50–80% in both species. However, other graminicides, including the cyclohexanediones and the chloroacetamide alachlor, had no affect. Similarly, auxin-type herbicides such as 2,4-D and MCPA did not inhibit H⁺-ATPase activity. Results are discussed in relation to the proposed mode of action of these herbicides. © 1998 SCI     

An investigation to enhance understanding of the stimulation of weed seedling emergence by soil disturbance

Enhanced understanding of soil disturbance effects on weed seedling recruitment will help guide improved management approaches. Field experiments were conducted at 16 site‐years at 10 research farms across Europe and North America to (i) quantify superficial soil disturbance (SSD) effects on Chenopodium album emergence and (ii) clarify adaptive emergence behaviour in frequently disturbed environments. Each site‐year contained factorial combinations of two seed populations (local and common, with the common population studied at all site‐years) and six SSD timings [0, 50, 100, 150, 200 day‐degrees (d°C, base temperature 3°C) after first emergence from undisturbed soil]. Analytical units in this study were emergence flushes. Flush magnitudes (maximum weekly emergence per count flush) and flush frequencies (flushes year^?1) were compared between disturbed and undisturbed seedbanks. One year after burial, SSD promoted seedling emergence relative to undisturbed seedbanks by increasing flush magnitude rather than increasing flush frequency. Two years after burial, SSD promoted emergence through increased flush magnitude and flush frequency. The promotional effects of SSD on emergence were strongest within 500 d°C following SSD; however, low levels of SSD‐induced emergence were detected as late as 3000 d°C following SSD. Accordingly, stale seedbed practices that eliminate weed seedlings should occur within 500 d°C of disturbance, because few seedlings emerge after this time. However, implementation of stale seedbed practices will probably cause slight increases in weed population densities throughout the year. Compared with the common population, local populations exhibited reduced variance in total emergence measured within sites and across SSD treatments, suggesting that C. album adaptation to local pedo‐climatic conditions involves increased consistency in SSD‐induced emergence.     

Life history parameters of Tetranychus cinnabarinus on glasshouse plants1

During the last few years, mass outbreaks of Tetranychus cinnabarinus have occurred on different host plants in Poland. The most heavy damage has been noted on glasshouse tomatoes, but the species was frequent on other host plants such as cucumber, capsicum, aubergine and gerbera. The aim of the study was to estimate the potential of development of T. cinnabarinus on different host plants. Demographic parameters such as intrinsic rate of natural increase, net reproductive rate, and the mean generation time, have been used as criteria of life-history traits on glasshouse tomato, cucumber, capsicum and gerbera. The relationships between the difference in life-history parameters of T. cinnabarinus and the different degree of damage on these host plants are discussed.     

不同N、P、K配比对小麦、玉米光合生理及周年水分利用的影响

为探明不同N、P、K配比在等灌水量条件下对小麦、玉米光合生理特征及周年水分利用的机理,采用大田实验,研究了不同N、P、K配比对小麦、玉米光合生理、产量及水分利用等的影响。结果表明:在小麦、玉米关键生育期进行适量灌水并进行追肥,促进了小麦、玉米对水分和养分的吸收,实现水肥同步,改善其光合生理特征,促进节水增产。不同处理均显著提高了小麦和玉米的光合速率和叶片水分利用效率,且均以N3P2K2处理的光合速率和叶片水分利用效率最高(小麦分别为16.59μmol·m~(-2)·s-1和36.07μmol·mmol-1,玉米分别为29.26μmol·m~(-2)·s-1和27.1μmol·mmol-1)。同时,不同N、P、K配比均提高了小麦、玉米的成产要素。最终,以N2P2K2和N3P2K2处理的增产和节水效果最佳,其小麦、玉米单产和二者总产均高于其它处理,其小麦、玉米总产量分别较对照提高了30.3%和26.8%,周年水分利用效率分别较对照提高了30.5%和27.4%。而从经济的角度考虑,本文推荐的N、P、K配比为N2P2K2。     

喷雾参数对自走式喷杆喷雾机稻田喷雾农药利用率及雾滴沉积分布的影响

比较在不同喷雾压力和施药液量条件下,采用自走式喷杆喷雾机在水稻分蘖期、孕穗期、扬花期进行喷雾处理的农药利用率、沉积分布均匀性及水稻茎基部雾滴密度的差异,为建立自走式喷杆喷雾机在稻田的高效施药技术提供理论依据。以生物染料丽春红-G作为农药示踪剂,估测不同喷雾参数的农药利用率,并用变异系数、绝对份额比例值比较农药分布的均匀性。同时通过水敏纸收集水稻基部雾滴密度,分析不同喷雾参数下农药雾滴穿透水稻冠层的能力。结果表明:在水稻分蘖期,采用自走式喷杆喷雾机在喷雾压力为1.2 MPa、施药液量为375 L/hm^2条件下喷雾,每个采样点的丽春红-G的平均沉积量仅为0.11 mg,与喷雾压力为0.4 MPa、施药液量为300 L/hm^2条件下喷雾的沉积量相比减少了56.00%。在水稻孕穗期,当喷雾压力为1.2 MPa、施药液量为375 L/hm^2时进行喷雾处理,每个采样点的丽春红-G的平均沉积量达0.26 mg,显著高于其他4个处理。在水稻扬花期,不同喷雾参数间的沉积量差距减小。在合适的喷雾压力和施药液量时,自走式喷杆喷雾机在水稻分蘖期、孕穗期和扬花期进行喷雾处理,农药利用率分别达到40.57%、54.97%和55.50%。综合变异系数和绝对份额比例两个指标,采用自走式喷杆喷雾机在喷雾压力为0.8 MPa、施药液量为300 L/hm^2条件下喷雾,农药分布更均匀。喷雾压力对水稻基部雾滴密度有显著影响,在水稻生长中后期,当喷雾压力低于0.8 MPa、施药液量为225~375 L/hm^2时,水稻茎秆基部雾滴密度均小于30个/cm^2。研究结果表明,喷雾压力和施药液量对自走式喷杆喷雾机稻田喷雾的农药利用率、分布均匀性及水稻茎秆基部雾滴密度有显著影响。在水稻生长前期,不宜采用高压力大水量喷雾作业;在水稻生长中后期,为增加对水稻基部病虫害的防治效果,需提高喷雾压力。