柴达木山羊血清生化指标测定

对柴达木山羊、引进的辽宁绒山羊及改良绒山羊的１４项血不生化指标进行了测定。结果;结果：三种山羊的血清钾、钠、钙、氯和无机磷浓度相近似;改良绒山羊的ＧＰＴ和ＧＯＴ活性高于辽宁绒山羊;辽宁绒山羊和改良绒山羊的ＺｎＴＴ和ＴＴＴ相近似;柴达木山羊和改良绒山羊的血清总蛋白含量低于辽宁绒山羊,而柴达木山羊的Ａ／Ｇ比值显著高于辽宁绒山羊和改良绒山羊。     

岩羊血清生化指标的测定

对青海省１３只岩羊的１２项血清生化指标进行了测定。结果：①岩羊的血清钠特别高，为２１７．３３ｍｍｏｌ／Ｌ，其它指标与家养的山羊和绵羊大致相同；②野生岩羊的ＧＯＴ非常显著地高于动物园岩羊（Ｐ〈０．０１），血清氯显著低于动物园岩羊（Ｐ〈０．０５）。     

京白蛋鸡耐热力评定指标的研究

本试验研究了高温对鸡血清血糖（ＧＬＵ）、胆固醇（ＣＨＯＬ）、总蛋白（ＴＰ）、清蛋白（ＡＬＢ）、球蛋白（ＧＬＯＢ）、碱性磷酸酶（ＡＬＰ）,谷丙转氨酶（ＧＰＴ）、谷草转氨酶（ＧＯＴ）,肌酸磷酸激酶（ＣＰＫ）,乳酸脱氢酶（ＬＤＨ）、和血浆三磺甲腺原氨酸（Ｔ３）等１１项血液生理生化指标的影响,分析了耐热力（ＨＳＳＴ）与耐热指数（ＩＨＴ）及１１项血液生理生化指标的相关,探讨了以上指标作为耐热指标的可能性,结     

日粮中添加不同抗氧化剂对奶牛血液生化指标的影响

选用２０头荷斯坦干乳母牛，随机分为４个组，研究日粮中添加二氢吡啶和维生素Ｅ对奶牛血液中一些主要生化指标的影响。结果表明，日粮中添加二氢吡啶、维生素Ｅ或两者同时添加，能使产后母牛血清中谷草转氨酶（ＧＯＴ）活性显著降低而碱性磷酸酶（ＡＫＰ）活性显著提高，谷丙转氨酶（ＧＰＴ）活性和血糖浓度有所升高，而血清总蛋白浓度有所下降，对乳酸脱氢酶（ＬＤＨ）、血总脂和血钙含量无明显影响。     

日粮中添加不同抗氧化剂对奶牛血液生化指标的影响

选用２０头荷斯坦干乳母牛，随机分为４个组，研究日粮中添加二氢吡啶和维生素Ｅ对奶牛血液中一些主要生化指标的影响。结果表明，日粮中添加二氢吡啶、维生素Ｅ或两者同时添加，能使产后母牛血清中谷草转氨酶（ＧＯＴ）活性显著降低而碱性磷酸酶（ＡＫＰ）活性显著提高，谷丙转氨酶（ＧＰＴ）活性和血糖浓度有所升高，而血清总蛋白浓度有所下降，对乳酸脱氢酶（ＬＤＨ）、血总脂和血钙含量无明显影响     

青海高原珍贵野禽血清生化指标测定

对青海高原５种珍贵野禽１２项血清生化指标进行了测定，结果发现被检野禽的血清生化指标有以下特征：（１）黑颈鹤和白天鹅的血清总蛋白较低（２６．０～３０．０ｇ／Ｌ）（２）各种野禽的Ａ／Ｇ值相差悬殊，其中斑头雁高达４．４２，蓝马鸡只有０．８１；（３）血清无机磷和钾离子浓度较低（分别为０．４６－１．２３ｍｍｏｌ／Ｌ，１．９－２．９５ｍｍｏｌ／Ｌ）；（４）血清酶活性在各种野禽之间相差甚大     

莱茵鹅15项血清生化指标的测定及分析

为了更有效地保护和利用莱茵鹅的优良基因,对北方种鹅场和双鸭山种鹅场的莱茵鹅血清进行了15项生化指标的测定及分析。结果表明:北方种鹅场和双鸭山种鹅场莱茵鹅血清三酰甘油、肌酐、直接胆红素、氯、镁、尿素氮、白蛋白和钙等血清生化指标之间的差异达到了极显著水平(P<0.01);血清总蛋白和总胆红素之间的差异达到了显著水平(P<0.05);而葡萄糖、高密度脂蛋白胆固醇、总胆固醇、无机磷和尿酸含量之间差异不显著(P>0.05)。研究还初步建立了莱茵鹅15项血清生化指标的参考值及参考范围。     

河南大尾寒羊主要血液生化指标的测定

对41只河南大尾寒羊(母19只,公22只)血清总蛋白,血清白蛋白,血清球蛋白,血清总胆固醇,血糖,血清钾,血清钠,血清钙,血清尿素氮和肌酐共10项血液生化指标进行了测定。结果表明:除血清胆固醇指标公羊极显著高于母羊外(P<0.01),其它各项指标公母羊均无显著差异(P>0.05)     

电针麻醉对绵羊生理生化指标的影响

针刺绵羊百会、三台二穴，研究电针麻醉对心率、呼吸频率、体温、血糖、血清谷转氨酶（ＧＯＴ）、谷丙转氨酶（ＧＰＴ）等生理生化指标的影响。结果表明，电针麻醉使心率略有下降，体温、呼吸频率有所上升；电针麻醉过程中血糖含量上升；ＧＯＴ、ＧＰＴ含量变化不显著。     

三种核型家猪10项生化指标测定

按常方法制备血清样本,用日立７０５型全自动生化分析仪对经核型鉴定的１６头１３／１７易位纯合子猪、２０头１３／１７易位杂命了猪和２０头正常核型家猪等三种型家猪的１０项生化指标进行了测定。结果表明,在３～４月龄阶段碱性磷酸酶（ＡＬＤ）、胆碱脂酶（ＣＨＥ）、血清甘油三酯（ＴＧ）三项指标,１３／１７易位纯合子猪都显著正常核型家猪及１３／１７易位杂合子猪（Ｐ〈０．０１）,尽管纯合子与杂合子之间,杂合子与正常     

不同能量和蛋白质水平日粮对妊娠期湘东黑山羊血液生化指标的影响

为研究不同能量和蛋白质水平日粮对妊娠期湘东黑山羊血液生化指标的影响,选取中等体况(30 kg±4 kg)的湘东黑山羊120只进行同期配种,再从怀孕母羊中挑选100只分为10组,每组10只,根据母羊妊娠生理阶段,将其分为妊娠前期(前90 d)和妊娠后期(后60 d)两个阶段进行饲喂试验,以能量和蛋白质作为因子,蛋白水平设A、B、C、D、E五个组,梯度为10 g;能量水平设V、W、X、Y、Z五个组,梯度为0.5 MJ。结果表明:日粮不同能量水平对妊娠母羊血糖、胰岛素和甘油三酯含量均有明显影响;日粮不同蛋白质水平对妊娠母羊血清尿素氮和总蛋白均有明显影响。     

Analysis on Dynamic Changes of Nutrient Intake and Serum Biochemical Indexes of Barn Feeding Ewes

In order to research the dynamic changes of nutrient intake and serum biochemical indexes of barn feeding ewes in different pregnant stages, and provide evidence for reasonable diet formulation and health state analysis of barn feeding goats, each 10 healthy female goats in non-pregnancy, early pregnancy and late pregnancy were randomly selected in this experiment, whose feed intake were determined;6 ill parturient ewes and each 8 healthy female goats in non-pregnancy, 1 month pregnancy, 2 months pregnancy, 3 months pregnancy, 4 months pregnancy and labor were randomly selected in this experiment, whose 7 serum biochemical indexes were determined.The results showed as follows:① In comparison to NRC(2007) goat nutritional requirements, DM, ME and CP intakes were more than the requirements in non-pregnant and early pregnant ewes, while DM, ME and CP intakes were less than the requirements in late pregnant ewes;Ca and P intakes were much more than the requirements in various stages of ewes.② From non-pregnancy, pregnancy to labor, healthy ewes' GLU content decreased (3.44 to 2.57 mmol/L) and KB content increased (14.93 to 23.55 μg/mL), while ill parturient ewes'GLU content was significantly decreased to 0.85 mmol/L(P<0.05) and KB content was significantly increased to 42.98 μg/mL (P<0.05);Healthy ewes' TP content decreased (70.55 to 59.94 g/L) and ALB content had little change (33.55 to 32.61 g/L), while ill parturient ewes' TP and ALB content both further decreased (56.79 and 29.72 g/L, respectively);Healthy ewes' Ca and P content had little change (2.21 to 2.19, 2.62 to 2.25 mmol/L, respectively), while ill parturient ewes' Ca (1.80 mmol/L, P<0.05) and P (2.17 mmol/L) content both decreased;Healthy ewes'AKP content decreased (258.57 to 104.35 U/L), but ill parturient ewes'AKP content increased (180.53 U/L).All the analysis showed that the inadequate energy intake of ewes in late pregnancy was the root cause of the decreased GLU and other serum biochemical indexes contents and the increased KB contents and the ill parturient ewes was diagnosed as pregnancy toxemia.Therefore, the ewes'diet formulation should be adjusted, different stages of ewes should be separated to feed, the energy intake of ewes in late pregnancy should be increased.     

舍饲母羊养分摄入量与血清生化指标动态变化分析

本试验通过研究不同妊娠期母羊在舍饲条件下养分摄入量与血清生化指标的动态变化,为舍饲山羊日粮的合理配制和健康状态分析提供依据。随机选择空怀期、妊娠前期和妊娠后期健康母山羊各10只,测定其采食量;随机选择空怀期,妊娠1、2、3、4月及临产健康母羊各8只,以及临产病母羊6只,测定其7项血清生化指标。结果表明:①与NRC(2007)山羊营养需要量相比,空怀期和妊娠前期母羊对干物质、代谢能和粗蛋白质的摄入量高于需要量,但妊娠后期母羊相应的摄入量低于需要量;各阶段母羊对钙和磷的摄入量均远高于需要量;②从空怀、妊娠到临产,母羊的血清葡萄糖含量逐渐下降(3.44至2.57 mmol/L),血清酮体含量逐渐升高(14.93~23.55 μg/mL),而临产病母羊的血清葡萄糖含量显著降至0.85 mmol/L(P<0.05),血清酮体含量显著升至42.98 μg/mL (P<0.05);血清总蛋白含量呈下降趋势(70.55至59.94 g/L),但血清白蛋白含量(33.55至32.61 g/L)变化不大,临产病母羊血清总蛋白(56.79 g/L)和白蛋白(29.72 g/L)含量进一步降低;血清钙(2.21至2.19 mmol/L)和磷(2.62至2.25 mmol/L)含量变化不大,而临产病母羊的血清钙(1.80 mmol/L)含量显著降低(P<0.05),血清磷含量下降至2.17 mmol/L;血清碱性磷酸酶活性呈下降趋势(258.57至104.35 U/L),但临产病母羊的血清碱性磷酸酶活性突然升高至180.53 U/L。综上所述,妊娠后期母羊能量摄入不足,是导致妊娠母羊血清葡萄糖等逐渐降低、酮体逐渐升高的根本原因,临产病母羊诊断为妊娠毒血症,建议调整母羊日粮结构,分阶段饲喂,增加妊娠后期母羊能量摄入量。     

携带传染性鼻气管炎病毒牦牛肝功能指标检测

采用生物化学方法对34份携带传染性鼻气管炎病毒（IBRV）牦牛（阳性组）血清和18份健康牦牛（阴性组）血清的肝功能指标进行了测定。 结果表明: 阳性组血清的总蛋白、球蛋白、β 球蛋白显著低于阴性组（P<0.05）;而AST、ALT及AST/ALT 均高于阴性组血清,差异极显著（P<0.01）,且部分阳性牛的肝脏损害严重（11.77%）。     

Isoenzymes of alkaline phosphatase in serum of lambs and ewes.

Electrophoresis in polyacrylamide gels was used to identify isoenzymes of serum alkaline phosphatase in sheep. Skeletal isoenzyme predominated in the serum of lambs and liver, skeletal and intestinal isoenzymes were found in the serum of adult ewes. In r ewes (R-r-i blood group system) intestinal isoenzyme activity was 67 per cent of total serum activity; in R ewes intestinal activity was only 36 per cent of total activity. Relative activities of the three isoenzymes differed greatly within individual ewes and between individual ewes during pregnancy and lactation.     

青海省湟源县本地黄牛四项血清钙指标的测定

对30头湟源县本地黄牛的4项血清钙指标进行了测定。结果表明：犊牛的血清总钙、血清结合钙和血清游离钙含量均显著高于成年牛（P＜0．01），而两者的血清游离钙百分率相似（P＞0．05）。     

1例自发性糖尿病猕猴血液学及血液生化指标分析

通过测定和分析自发性糖尿病猕猴的血液学、生化指标,为建立猕猴自发性糖尿病模型提供参考数据。对发生类似人类糖尿病“三多一少”症状的1只猕猴,应用全自动血细胞分析仪和血液生化分析仪,测定其血液学、生化值,并进行糖耐量试验。结果血糖(空腹)、尿糖和尿素显著升高;甘油三酯、肌酐、总胆固醇、氯、磷、镁和二氧化碳处于正常值高限或增多,血液学中性粒细胞百分比、血红蛋白、平均红细胞血红蛋白和平均红细胞血红蛋白浓度升高。表明猕猴为典型的自发性糖尿病动物。     

全混合颗粒料中添加不同类型蛋氨酸对湖羊生长性能及血清生化指标的影响

本试验旨在研究在湖羊全混合颗粒料中添加不同类型蛋氨酸(蛋氨酸钙盐与蛋氨酸羟基类似物异丙酯)对湖羊生长性能及血清生化指标、抗氧化指标和激素含量的影响.选取体重为(19.99±2.04)kg的健康湖羊72只,随机分为3组:对照组、试验Ⅰ组(在基础饲粮中添加1.5 kg/t蛋氨酸钙盐)和试验Ⅱ组(在基础饲粮中添加1.5 kg...     

有机酸和精油复合微囊包被物对肉鸡生长性能、免疫器官指数、屠宰性能、肉品质和血清生化指标的影响

本试验旨在研究有机酸和精油复合微囊包被物(MOAEO)对肉鸡生长性能、免疫器官指数、屠宰性能、肉品质和血清生化指标的影响。选取1日龄黄羽肉公鸡300只,随机分成5个组,每个组5个重复,每个重复12只。Ⅰ组为对照组,饲喂基础饲粮;Ⅱ组为抗生素组,在基础饲粮基础中添加30 mg/kg杆菌肽锌和6 mg/kg硫酸黏杆菌素;Ⅲ、Ⅳ、Ⅴ组为试验组,分别在基础饲粮中添加150、200、250 mg/kg的MOAEO。试验期70 d。结果表明:1)1~21日龄,与对照组相比,Ⅱ组平均日增重显著提高(P0.05),Ⅳ组料重比显著降低(P0.05),Ⅳ、Ⅴ组脾脏指数显著提高(P0.05),Ⅴ组血清尿酸含量显著降低(P0.05)。2)1~70日龄,与对照组相比,Ⅳ组料重比显著降低(P0.05),Ⅳ组脾脏指数显著提高(P0.05),Ⅱ、Ⅳ组血清总蛋白含量显著提高,Ⅱ、Ⅳ组血清尿酸含量显著降低(P0.05),Ⅳ组胸肌率和腿肌率显著提高(P0.05),Ⅳ组胸肌失水率显著降低(P0.05)。3)与抗生素组相比,饲粮添加MOAEO对肉鸡生长性能、免疫器官指数、屠宰性能和血清生化指标的影响不显著(P0.05)。由此可见,肉鸡饲粮中添加MOAEO能改善肉鸡生长性能、免疫器官指数、屠宰性能、肉品质和血清生化指标,MOAEO适宜添加量为200 mg/kg。     

Electrophoretic serum protein fraction profile during the different physiological phases in Comisana ewes

The aim of this study was to evaluate the influence of different physiological phases on serum total proteins and their fractions of ten Comisana ewes housed in Mediterranean area. From each animal, blood samples were collected at different physiological phases: late pregnancy, post-partum, early, mid-, end lactation and dry period. On all samples serum total proteins were determined by the biuret method, and albumin, α-globulins, β(1) -globulins, β(2) -globulins and γ-globulins concentrations were assessed using an automated system. One-way repeated measures analysis of variance was applied to determine the significant effect of different physiological phases on the parameters studied. During the late pregnancy and post-partum, total proteins, β1- and β2-globulins and γ-globulins showed the highest values. Starting from post-partum, α-globulins increased to reach their peaks in mid-lactation. Early lactation was characterized by low γ-globulins values. The increase in serum albumin concentration and the drop in some globulin fractions determined the significant increase in albumin/globulin ratio. The obtained results contributed to improve the knowledge on electrophoretic profile during the different physiological phases in ewes, confirming that pregnancy and lactation periods affect the protein metabolism. Particularly, serum protein fractions pattern could give information about dehydration, plasma volume expansion and hepatic function, which occur during the different physiological phases. Dynamics of the protein profile - from pregnancy to dry period - which are provided by our results, could be considered as guidelines for the management strategies to guarantee the nutritional needs of these animals during the different physiological phases and to avoid a decline of productive performance and consequently an economic loss.