Kindlin-2与VEGF-C蛋白在胃癌组织中的表达及意义

目的:检测胃癌组织中Kindlin-2与VEGF-C蛋白的表达,探讨二者在胃癌发生发展中的作用。方法:选取109例胃癌组织标本为研究对象,采用免疫组织化学染色方法检测Kindlin-2与VEGF-C的表达。结果:Kindlin-2在54.1%的胃癌组织中表达,与肿瘤TNM分期和淋巴结转移密切相关,并发现Kindlin-2表达与VEGF-C表达在胃癌组织中呈正相关(P<0.05)。结论:Kindlin-2和VEGF-C可能在胃癌细胞侵袭过程中协同发挥作用。     

MTS1在食管鳞状细胞癌组织中的表达研究

目的 :研究肿瘤相关基因(multiple tumor suppressor,MTS1)在食管鳞状细胞癌中的表达及意义。方法 :应用免疫组化S.P法检测食管鳞状细胞癌患者60例,观察肿瘤转移相关基因MTS1的表达与食管鳞状细胞癌发生及浸润、转移的关系。结果:1)60例食管鳞状细胞癌组织中,MTS1的表达阳性检出率为:31.7%(19/60);2)有和无淋巴结转移的食管鳞状细胞癌组织中MTS1的阳性表达检出率分别为73.7%和12.2%,两者比较,P0.05,统计学差异有显著性。结论 :MTS1的失活对食管鳞状细胞癌浸润转移可能起着重要作用。     

TBX3在胃癌组织中的表达及临床意义

目的 :探讨胃癌组织中TBX3的表达水平及其与临床病理特征的相关性。方法:采用免疫组织化学对130例胃癌组织中TBX3的表达进行检测,分析其与肿瘤临床病理特征的相关性。结果:胃癌组织中TBX3阳性表达率为36.2%(47/130),TBX3在胃癌组织中的阳性表达在不同胃癌大小组间差异有统计学意义。TBX3在胃癌组织中的阳性表达与患者性别、年龄、病理分型、肿瘤部位、淋巴结有无转移、静脉内有无癌栓、神经有无侵犯均无统计学意义(P>0.05)。结论:TBX3可能涉及促进胃癌的进展、侵袭、转移,可作为提示胃癌患者的有效预后预测因子之一。     

犬乳腺肿瘤组织病理学观察及腱糖蛋白C表达的检测

为了探讨腱糖蛋白C(TNC)表达与犬乳腺肿瘤生物学关系,对23例犬乳腺肿瘤临床病例进行病理学观察及分类,然后采用免疫组化SABC方法检测TNC蛋白在23例犬乳腺肿瘤和2例犬正常乳腺组织中的表达情况。结果显示:23例病例中,良性肿瘤有7例,占30.43%;恶性肿瘤有13例,占56.52%;增生病变有3例,占13.05%。在良性肿瘤中良性混合瘤最多,为3例,其余2例为导管内乳头状腺瘤,1例复杂腺瘤及1例纤维腺瘤。恶性肿瘤中,5例未分化癌,3例癌肉瘤,2例筛状癌,2例固体癌,1例纤维肉瘤,1例混合瘤引起的癌。TNC蛋白主要表达于细胞外基质。犬恶性乳腺肿瘤中,TNC蛋白阳性表达率为46.15%(6/13);良性乳腺肿瘤中,其阳性表达率为42.85%(3/7);乳腺增生病变和正常乳腺组织中,未见其表达。研究结果表明,TNC蛋白可以为犬乳腺肿瘤的鉴别诊断提供参考。     

滨州地区青年人胃癌临床病理特征分析

目的:探讨滨州地区青年人胃癌的临床病理特点,提高青年人胃癌诊疗水平,以达到早发现、早诊断及有效治疗的目的。方法:收集滨州医学院附属医院2010年11月-2016年12月滨州医学院附属医院收治的60例青年人胃癌患者及221例中老年人胃癌,对两组人群胃癌临床病理特点、耐药相关蛋白、P53免疫表型进行分析比较。结果:青年组与老年组胃癌比较,弥漫型更多见,肿瘤大小较老年组小,耐药相关蛋白MDR-1在青年组与中老年组均有表达,青年组中阳性表达更高,差异有统计学意义(P<0.05)。结论:滨州地区青年组胃癌分化更低,恶性程度高,耐药性相关蛋白呈高表达状态,后续治疗耐药发生率更高,对青年人胃癌应更加重视,早期诊断、及时治疗是关键。     

PPARγ和PCNA在口腔鳞癌组织的表达及临床意义

目的 探讨过氧化物酶体增殖物激活受体(PPARγ)与增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)在口腔鳞癌中(oral squamous-cell carcinoma,OSCC)的表达及其生物学意义.方法 采用免疫组织化学和流式细胞术检测50例口腔鳞状细胞癌、癌旁黏膜(距瘤缘2 cm)和切缘正常黏膜(距瘤缘≥5 cm,镜下未见癌浸润)标本中PPARγ和PCNA的表达.结果 PPARγ在癌组织中的阳性表达率为68.0% (34/50),高于癌旁组织(26.0%,13/50)和正常黏膜组织(2.0%,1/50) (P ＜0.05);PCNA在癌组织中的阳性表达率为72.0% (36/50),高于癌旁组织(26.0%,13/50)和正常黏膜组织(4.0%,2/50)(P＜0.05).流式细胞术检测PPARγ在鳞癌组织中的表达为(396.56±33.39),明显高于癌旁组织(333.36±15.93)及正常口腔黏膜组织(289.28±13.80)(P＜0.05).PCNA在鳞癌组织中的表达为(543.11±41.55),明显高于癌旁组织(456.97±23.86)和正常口腔黏膜组织(409.40±40.07) (P ＜0.05).PPARγ和PCNA的表达与细胞分化程度、淋巴结转移均有相关性(PPARγ,r分别为0.497、0.392,P＜0.05;PCNA,r分别为0.324、0.561,P＜0.05),且二者在口腔鳞癌中的表达呈正相关.结论 口腔鳞癌组织中PPARγ和PCNA的表达均升高,可能与口腔鳞癌的发生、发展相关.     

去乙酰化蛋白酶1在马兜铃酸肾纤维化模型中对TGF-β1/Smad7信号通路的作用

检测马兜铃酸肾纤维化(AAN)模型肾组织中转化生长因子-p1 (TGF-β1)、Smad7、去乙酰化蛋白酶1(HDAC1)的mRNA和蛋白表达变化,探讨HADC1和Smad7的表达在AAN发展中的作用及两者间的联系.RTPCR检测TGF-β1、Smad7和HDAC1的mRNA表达,ELASA检测TGF-β1的蛋白表达,免疫组化定位和检测HADC1和Smad7蛋白,HE染色观察肾病理学改变.结果显示:AAN模型肾组织中TGF-p1和HDAC1mRNA表达呈时间依赖性升高,TGF-p1 mRNA表达较对照组有明显差异(P＜0.05),28 d开始AAN组中HDAC1mRNA增加,差异极显著(P＜0.01) ;Smad7 mRNA和蛋白表达均呈下降趋势,HDAC1蛋白免疫组化染色强度呈上调趋势,与对照组比较HDAC1阳性着色主要分布于上皮细胞和间质细胞的细胞核;肾脏病理表现为肾小管浊肿、变性和脱落等急性小管间质损伤,并随用药时间的延长呈进行性加重.本研究表明肾组织中Smad7弱表达可促进TGF-β1信号转导,HDAC1 mRNA和蛋白在肾组织中的高表达并参与TGF-β1/Smad7信号通路在肾纤维化发展中起重要作用,可能是潜在的新作用靶点.     

一例犬膀胱小细胞癌的诊断

犬膀胱小细胞癌,是一种分化程度较低、具有高度浸润转移能力、预后差的恶性肿瘤。2012年诊断一例膀胱小细胞癌病例,通过对该病例的临床诊断,对B超检查和组织采集方法进行了创新并通过组织病理切片诊断,现作一报告。1病例介绍2012年1月4日,收治一例2岁雌性金毛犬,体重30kg,一年前由美国进境来北京,近半年来出现尿频、尿血。经抗生素治疗无效,精神状态正常、饮食尚可。     

1例猫肾上腺皮质癌脾转移的病理及免疫组化分析

猫的肾上腺皮质癌转移至脾在兽医临床非常罕见。在本报告中,描述了1例10岁田园猫,其症状表现为腹部疼痛,频繁呕吐,消瘦,有慢性肾上腺功能损伤病史,影像学检查脾肿大。对患猫进行脾摘除手术,并对摘除的组织进行组织病理学检查和免疫组化分析。病理组织学结果显示,肿瘤细胞具有丰富的脂质样空泡,细胞体积大,有丝分裂象多见,且肿瘤细胞已发生血管侵袭,细胞核浓染;免疫组织化学结果显示,synaptophysin、S-100和GATA4阳性表达。根据病理组织学和免疫组织化学及病史和实验室检查,最后诊断为肾上腺皮质癌的脾转移。首次报道了猫肾上腺皮质癌脾转移临床病例及病理学诊断,对于临床相关疾病诊疗有一定的参考价值和借鉴意义。     

胃窦黏膜肠上皮化生腺体中LAPTM4B蛋白的表达及其意义

本研究通过免疫组织化学染色方法检测了80例胃窦黏膜中LAPTM4B蛋白表达情况,并结合形态学观察判断肠上皮化生的类型及程度。80例标本中28例检测到LAPTM4B蛋白在肠上皮化生腺体表达(35%),52例未检测到LAPTM4B蛋白在肠上皮化生腺体表达(65%)。LAPTM4B蛋白在不同类型、不同程度肠上皮化生中的表达有显著性差异(P<0.01)。LAPTM4B基因的异常表达可能在胃癌发生的早期阶段发挥重要作用。     

补中益气丸对糖尿病大鼠胃组织MUC1、COX、NOS表达变化及相互作用

观察补中益气丸对糖尿病大鼠胃组织MuC1、COX-1、COX-2、cNOS、iNOS不同病程表达变化及其相互作用的影响、大鼠随机分为正常组。模型组和中药组,采用STZ腹腔注射结合乙醇灌胃的方法建立糖尿病大鼠胃黏膜损伤模型．检测胃组织NO、PGE2、6-ketoPC-F1d、MuCl、COX-1、cOX2、cNOS和iNOs随病程的表达变化及其相互作用。结果显示,糖尿病大鼠出现胃黏膜损伤和胃肠功能紊乱,胃组织MUC1、COX2、iNOS发生显著变化．MUC1随病程进展逐渐降低,iNOs和COX-2表现为早期活性降低,晚期活性升高,PGE2、6-keto—PGF1d和NO随病程显著升高,相关分析发现．COX1与cNOS呈显著正相关,与iNOS呈著负相关。补中益气丸能调节MUC1、COX-2、iNOS的表达及其相互作用,阻止胃黏膜损伤。结果表明,STZ腹腔注射结合乙醇灌胃的方法可成功诱导糖尿病大鼠胃黏膜损伤模型,iNOS与COX2可能为相互协作和相互介导的关系,在糖尿病胃黏膜损伤方面起重要作用,补中益气丸通过升高MUC1、降低iNOS和COX-2的表达及其相互作用,阻止胃黏膜损伤和糖尿病的发展。     

Expression of gastric ghrelin and H(+)-K(+)-ATPase mRNA in weanling piglets and effect of ghrelin on H(+)-K(+)-ATPase expression and activity in gastric mucosal cells in vitro

This study was designed to investigate the effect of ghrelin on gastric acid secretion in weaning piglets both in vivo and in vitro. Thirty newborn piglets were selected from six litters and on 28, 35 (weaning), 38, 42 and 45d of age, respectively, one piglet from each litter was killed and the mucosal tissue from gastric fundus was collected for detecting ghrelin mRNA as well as H(+)-K(+)-ATPase mRNA expression and activity. Primary cultures of gastric mucosal cells from 5-week-old weaning piglets were challenged with 3x10(-5), 3x10(-4), 3x10(-3), 3x10(-2) and 3x10(-1)nmol/ml h-ghrelin, respectively, for 4h in order to further clarify the effect of ghrelin on gastric H(+)-K(+)-ATPase mRNA expression and activity. Ghrelin mRNA expression in gastric fundus kept stable from 28d to 42d, followed by a sudden increase on 45d, exhibiting a peak that was significantly higher than any other age groups investigated. H(+)-K(+)-ATPase activity and mRNA expression showed similar trends of increase with slightly different timing. H(+)-K(+)-ATPase mRNA expression tended to increase on 42d, while H(+)-K(+)-ATPase activity started to rise from 35d, but neither of them reached significantly higher levels until 45d. In vitro, ghrelin significantly increased H(+)-K(+)-ATPase activity of gastric mucosal cells at 3x10(-4), 3x10(-3), and 3x10(-2)nmol/ml, but augmented H(+)-K(+)-ATPase mRNA expression only at 3x10(-4)nmol/ml. The results indicate that ghrelin mRNA expression is up-regulated 10 days after weaning in the gastric fundus of piglets, coinciding with the increase of H(+)-K(+)-ATPase mRNA expression and activity. Ghrelin acts on gastric mucosal cells to stimulate both mRNA expression and activity of H(+)-K(+)-ATPase in vitro.     

褪黑素受体MT1和MT2在绵羊胃中的分布及表达

【目的】应用分子生物学技术探究绵羊不同胃室组织褪黑素(MT)特异性膜受体MT1和MT2的分布规律及表达模式,以初步探明绵羊不同胃室组织褪黑素调节胃消化的生物效应机制。【方法】采集绵羊瘤胃背囊、瘤胃腹囊、网胃、瓣胃和皱胃5个组织部位,用ELISA、实时荧光定量PCR、免疫组化和Western blotting方法检测褪黑素特异性膜受体MT1和MT2在绵羊胃组织不同部位的分布规律及表达模式。【结果】ELISA结果显示,绵羊各胃组织中均含有褪黑素,且皱胃中褪黑素含量最高,瓣胃次之,瘤胃背囊、瘤胃腹囊和网胃中均较低。实时荧光定量PCR结果显示,MT1和MT2基因mRNA在皱胃中含量均最高,其次是瘤胃腹囊,在瘤胃背囊和网胃中含量较低。免疫组化结果显示,MT1和MT2蛋白在绵羊各胃组织中均有分布,主要表达于各胃组织的黏膜层,且在皱胃腺体中的分布呈从底部到颈部逐渐增多的趋势。Western blotting结果显示,MT1和MT2蛋白在网胃中表达均最高,瓣胃次之,在瘤胃背囊、瘤胃腹囊和皱胃中表达均较低。【结论】褪黑素在绵羊各胃组织中差异化表达从而发挥多样性生理功能,可能通过与胃组织上特异性受体MT1和MT2结合,通过信号传导系统而调控前胃受食糜刺激后发生反刍生理过程。     

Effect of hydrochloric acid,pepsin, or taurocholate on bioelectric properties of gastric squamous mucosa in horses

OBJECTIVE: To determine the effect of pH with or without pepsin or taurocholic acid on the bioelectric properties of gastric squamous mucosa in horses. SAMPLE POPULATION: Gastric tissues obtained from 16 adult horses that did not have evidence of gastric disease. PROCEDURE: Bioelectric properties of squamous mucosa were determined, using modified Ussing chambers. Tissues then were exposed to mucosal pepsin (1 mg/ml) or taurocholic acid (2.5 mM) under neutral (pH 74) or acidic (pH 1.7) conditions. RESULTS: Exposure of mucosal sheets to an acidic pH resulted in an immediate and sustained decrease in transmembrane potential difference and calculated tissue resistance. Pepsin or taurocholic acid did not significantly affect bioelectric variables when added to a mucosal bath solution of pH 7.4. A synergistic effect between pepsin or taurocholic acid and mucosal acidification was not detected. CONCLUSIONS AND CLINICAL RELEVANCE: Mucosal acidification with or without pepsin or taurocholic acid resulted in reduced tissue resistance. These data support the contention that squamous erosions or ulcers in horses are mediated, in part, by prolonged exposure of gastric squamous mucosa to luminal acid.     

半胱胺盐酸盐对仔猪胃黏膜细胞H+-K+-ATPase活性和生长抑素表达的影响

将分离的仔猪胃粘膜细胞分为4组，在含10％胎牛血清的DMEM高糖培养液中培养24h后，分别在新鲜的基础培养液中添加0（对照组）、0．001（低水平组）、0．01（中水平组）和0．1（高水平组）g／L的半胱胺盐酸盐，继续培养20h后收集细胞。测定细胞H^＋-K^＋-ATPase活性，同时用相对定量RT—PCR法测定细胞H^＋-K^＋-ATPase和生长抑素（SS）mRNA的相对丰度。结果表明：（1）低水平的半胱胺盐酸盐对H^＋-K^＋-ATPase的表达和活性没有影响（P〉0．05），但中、高水平的半胱胺盐酸盐显著提高了H^＋-K^＋-ATPase的表达和活性（P〈0．05），其中H^＋-K^＋-ATPase mRNA的相对丰度分别提高了36％和44％，H^＋-K^＋-ATPase活性分别提高了57％和53％。（2）低水平半胱胺盐酸盐对SSmRNA的表达没有影响（P〉0．05），中、高水平的半胱胺盐酸盐显著提高了SSmRNA的相对丰度（P〈0．05），提高幅度均为52％。以上结果提示半胱胺盐酸盐可增强体外培养的仔猪胃黏膜细胞H^＋-K^＋-ATPase的表达及活性，而这一作用可能由SS所介导。     

Effects of Firocoxib and Tepoxalin on Healing in a Canine Gastric Mucosal Injury Model

Background: Little is known about the effect of dual cyclooxygenase (COX) and lipoxygenase inhibition on canine gastric mucosal healing.
Objective: This study compares the effects of putative dual COX and 5-lipoxygenase inhibition with that of COX-2 selective inhibition on gastric mucosal lesion healing in dogs.
Animals: Six normal adult mixed-breed research dogs.
Methods: Gastric body and pyloric lesions were induced by endoscopic biopsy. Dogs were treated with tepoxalin, firocoxib, or placebo for 7 days in a randomized 3-way crossover study design. Healing was evaluated on days 2, 4, and 7 of treatment by endoscopic lesion scoring. Eicosanoid concentrations in plasma and at the lesion margins were determined on days 2, 4, and 7. Repeated measures analyses were performed. All hypothesis tests were 2-sided with P < .05. Multiple comparisons were adjusted using Tukey's test.
Results: Significant treatment differences were noted in the pyloric lesion area measurements. Overall, the firocoxib group had larger lesions than the placebo ( P = .0469) or tepoxalin ( P = .0089) groups. Despite larger pyloric lesions in the firocoxib group, mucosal prostaglandin production did not differ significantly from placebo. In contrast, the tepoxalin group had significantly lower pyloric mucosal prostaglandin production compared with the firocoxib ( P < .0001) or the placebo ( P < .0001) groups but pyloric lesions were not significantly larger than those of the placebo group ( P = .7829).
Conclusion: COX-2 inhibition by firocoxib slowed wound healing by a mechanism independent of prostaglandin synthesis. Suppression of mucosal prostaglandin production by tepoxalin did not alter mucosal lesion healing compared with placebo.     

Fluorescence In Situ Hybridization Confirms Clearance of Visible Helicobacter spp. Associated with Gastritis in Dogs and Cats

Background: The results of studies examining the role of Helicobacter spp. in the pathogenesis of canine and feline gastritis are inconclusive. Furthermore, data evaluating the effectiveness of medical therapy for eradication of Helicobacter infection are limited.
Aim: To detect Helicobacter spp. in mucosal biopsies of dogs and cats diagnosed with gastritis, with fluorescence in situ hybridization (FISH).
Animals: Three dogs and 2 cats with signs of chronic gastrointestinal disease.
Methods: Dogs and cats infected with Helicobacter spp. were treated with triple antimicrobial therapy and fed an elimination diet for 21 days. Helicobacter spp. status in endoscopic (3 dogs, 1 cat) or surgical biopsies (1 cat) of gastric mucosa was compared pre- and posttreatment in each animal by histology, FISH analysis, and polymerase chain reaction (PCR).
Results: Gastritis of varying severity with intraglandular spiral bacteria was observed in all animals. Pretreatment diagnostic tests confirmed the presence of mucosal Helicobacter spp. in all animals by FISH and histopathology and in 4/5 animals by PCR. Rapid resolution of vomiting episodes was observed in all animals. Gastric biopsies performed after triple therapy revealed clearance of visible Helicobacter spp. by histopathology and negative FISH analysis, as well as PCR in all animals.
Conclusions and Clinical Importance: Application of FISH to routine biopsy specimens enabled rapid and specific identification of Helicobacter spp. within the gastric mucosa of dogs and cats. Although medical therapy was useful in resolution of clinical signs and clearance of visible Helicobacter spp. in gastric biopsies, gastric inflammation persisted.     

Bone morphogenetic protein 4 (BMP‐4) and BMP‐7 induce vascular endothelial growth factor expression in bovine granulosa cells

Bone morphogenetic protein‐4 (BMP‐4) and BMP‐7, theca cell‐derived growth factors, directly affect the granulosa cell function. The aim of this study was to examine the involvement of BMP‐4 or BMP‐7 in vascular endothelial growth factor (VEGF) expression in bovine granulosa cells. Granulosa cells were collected from small follicles (4–6 mm) and seeded at a density of 2–5 × 10⁵ cells per well in Dulbecco's modified Eagle's medium (DMEM)/F12 medium with BMP‐4 or BMP‐7. The expression of VEGF messenger RNA and protein was the maximum when 1.0 ng/mL of BMP‐4 was added to the culture medium. On the other hand, 10 ng/mL of BMP‐7 significantly increased the expression of the VEGF gene and protein. In addition, BMP‐4 stimulated the expression of Smad1 and Smad5 genes in granulosa cells, whereas BMP‐7 stimulated the expression of Smad5 gene. These results suggested that BMP‐4 and BMP‐7 may be associated with VEGF expression via several specific Smads in bovine granulosa cells: BMP‐4 via Smad1/Smad5 and BMP‐7 via Smad5. In conclusion, theca cell‐derived BMP‐4 and BMP‐7 might contribute to follicular vasculature and development by inducing VEGF expression in granulosa cells.     

The effects of stress on gastric ulceration, T3, T4, reverse T3 and cortisol in neonatal foals.

Sixteen neonatal foals stressed by disease underwent endoscopic examination of their stomachs and blood was assayed for triiodothyronine (T3), reverse T3 (rT3), thyroxine (T4) and cortisol, to determine the effects of severe physiological stress and the occurrence of gastric ulcers. compared with eight age-matched controls, six foals had abnormal cortisol, seven had abnormal T3 and 12 had abnormal T4. Eleven of 13 foals had rT3 outside the 95 per cent confidence interval for clinically normal foals of comparable ages. Gastric lesions were seen more frequently in stressed foals, and gastric glandular mucosal lesions were noted in 40 per cent of the stressed foals. Previous studies report low (3 per cent) occurrence of gastric mucosal lesions. The frequency of squamous mucosal lesions was not different from that reported previously, indicating that stress has little effect on the development of lesions at this site.