Boron nutrition and mobility,and its relation to the elemental composition of greenhouse grown root crops. II radish

Abstract

The elemental distribution between the leaves and roots of mature radish (Raphanus sativus cv Cherry Belle) plants grown in the greenhouse with various concentrations of nutrient solution B or Ca was determined to assess the role of phloem in the provision of nutrients to the root, and the retranslocation of B under deficient conditions. The relative composition and accumulation of elements in different parts, and the ratio of their concentrations in leaves:roots were used as a measure of their uptake, relative mobility and retranslocation. The data indicate that B, but not Ca is retranslocated in the phloem to the roots when that particular element was in short supply in the nutrient solution. B deficiency induced brown heart disorder in radish roots but the severity was dependent on the degree of deficiency below 28 μg g^‐l DM in the root. These symptoms were alleviated when the root B concentrations were enhanced by foliar applications of B. It is concluded that radish responded to B deficiency in a fashion similar to that reported previously for rutabaga and that it might serve as a time‐saving model system for examining the mechanisms responsible for brown heart in rutabaga.     

脐橙幼苗新老叶片养分含量对大、中量元素短期缺乏的差异性响应

  【目的】  对比大、中量养分短期缺乏下脐橙新、老叶片中11种必需元素含量及变化,并分析缺素导致的营养元素间的相互影响。  【方法】  以一年生枳砧纽荷尔脐橙幼苗为试材进行了砂培试验。以完全营养液为对照 (CK),设置缺氮 (?N)、缺磷 (?P)、缺钾 (?K)、缺钙 (?Ca)和缺镁 (?Mg)处理,测定不同处理脐橙叶片（老叶和新叶）生长指标及矿质元素含量。  【结果】  所有缺素处理均导致叶片叶绿素含量降低,生物量减少,以缺氮处理最为显著。缺氮降低了叶片N、Ca、Cu、Mo含量;缺磷降低了叶片P、K、Mo含量;缺钾降低了叶片K含量;缺钙降低了叶片N、Cu、Zn、Mo含量但增加了P含量;缺镁降低了叶片Ca、Mg、Zn、Mo含量但增加了K含量。以必需矿质元素为变量分别对各处理老叶和新叶进行主成分分析,老叶中第一主成分 (PC1)明显将缺钾处理与其他处理区分开,与对照相比,缺钾老叶离子组成变化为N (?3%)、P (+1%)、K (?71%)、Ca (+11%)、Mg (+39%)、B (+16%)、Mn (+11%)、Fe (+32%)、Cu (?7%)、Zn (+14%)、Mo (?63%);新叶中PC1明显将缺氮处理与其他处理区分开,缺氮新叶离子组成变化为N (?53%)、P (+8%)、K (+7%)、Ca (?14%)、Mg (+11%)、B (+55%)、Mn (+51%)、Fe (?14%)、Cu (?57%)、Zn (+4%)、Mo (?25%)。老叶和新叶中元素含量呈正相关的元素是N-Cu、N-Ca、Mg-Mn和Cu-Mo,呈负相关的是K-Zn。  【结论】  脐橙幼苗老叶对钾的短期缺乏反应最敏感,缺钾会显著降低老叶中K和Mo含量并增加Mg和Fe含量,而新叶对氮素的短期缺乏最敏感,缺氮显著降低新叶中N、Ca、Cu和Mo含量。短期缺少P、Ca和Mg对脐橙幼苗叶片中的养分含量影响较小。     

Foliar Symptomology and Tissue Concentrations of Nutrient‐Deficient Vegetative Strawflower Plants

Abstract

Elemental deficiencies of nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, iron, manganese, copper, zinc, or boron (N, P, K, Ca, Mg, S, Fe, Mn, Cu, Zn, or B) were induced in plants of Florabella Pink strawflower [Bracteantha bracteata (Vent.) A. A. Anderberg]. Rooted stem cuttings were planted in 4.87‐L plastic containers and fertilized with a complete modified Hoagland's solution or this solution minus the element that was to be investigated. Plants were harvested for tissue analyses as well as dry weights when initial foliar symptoms were expressed and later under advanced deficiency symptoms. Deficiency symptoms for all treatments were observed within 7 weeks. The most dramatic expression of foliar symptoms occurred with N (chlorotic lower foliage leading to necrotic margins on the mature leaves), Ca (black necrotic spots on the tips of the young leaves), S (uniform chlorosis of young leaves and recently mature leaves), B (thick, leathery, and deformed young leaves), Fe (uniform yellowish‐green chlorosis on the young leaves), and Zn (brownish‐gray necrosis on the tips of the mature leaves). At the initial stage, only Fe‐deficient plants weighed less than the control, whereas K‐, Ca‐, and Mg‐deficient plants had greater dry weights than plants receiving the complete modified Hoagland's solution (control plants). Dry weights of plants treated with solutions not containing N, P, Ca, S, Cu, or Mn were significantly lower when compared with the control plants under an advanced deficiency. Foliar‐tissue concentration data will assist plant‐tissue analysis laboratories in establishing foliar symptom standards for growers.     

Growth and Nutrient Content of Trifoliate Orange Seedlings Influenced by Arbuscular Mycorrhizal Fungi Inoculation in Low Magnesium Soil

A pot experiment was conducted to examine the effects of arbuscular mycorrhizal fungi, Glomus versiforme, G. mosseae, and G. intraradices on growth and nutrition of trifoliate orange (Poncirus trifoliata) seedlings under magnesium (Mg)-nontreated and Mg-treated conditions. Whether treated with Mg or not, G. versiforme inoculation significantly enhanced the growth and concentrations of Mg, phosphorus, calcium, potassium, zinc, and copper in shoots or roots, and activities of acid phosphatase, catalase, invertase, and urease in rhizosphere soils. Additionally, there were higher levels of chlorophyll, proline, soluble sugar and protein in leaves, root viability, superoxide dismutase, peroxidase and catalase in leaves and roots, but lower malondialdehyde content in leaves and roots of mycorrhizal seedlings than non-mycorrhizal ones. Data demonstrated that G. versiforme-inoculated citrus seedlings exhibited higher levels of soil enzymes, osmoregulation, and antioxidant matters, leading to improvement of growth and nutrition of seedlings in low Mg soil.     

Acclimation of eggplant (Solanum melongena) to low boron supply

Root active uptake and remobilization of boron (B) have been accepted as mechanisms contributing to nutrient efficiency under low supply of boron. Here, we examined the existence of these mechanisms in eggplant (Solanum melongena L.) supplied either with luxury (100 μM, B+) or low (7.5 μM, B–) B in the growth medium via semihydroponic cultivation. Boron treatment was marginally not limiting growth thus avoiding side‐effects and impairment of acclimation mechanisms of plants. The induction of a B‐concentrating mechanism was evident in the roots as B concentration in the xylem sap was only decreased by 23% in B– compared to B+ plants, i.e., B– roots concentrated B by a factor of 2.7 relative to the external solution. Leaf B concentration in the B– treatment decreased by 33% and 40% in young fully expanded and mature leaves, respectively. Larger differences were observed in the soluble B fraction that decreased by 65% in mature leaves. However, both total and soluble B concentrations in developing leaves were almost equal for both treatments exhibiting a pattern commonly observed in B‐remobilizing plants. On the other hand, amounts of B export in the phloem sap were small compared to other species in which B is highly mobile. The B export rate from source leaves was slightly increased under low B supply while that of sucrose was not affected. We conclude that the root concentrating mechanism contributes to the alleviation of B deficiency in eggplant under low B supply while B remobilization may also contribute to a lower degree.     

Phytosiderophore release from manganese‐induced iron deficiency in barley

An experiment was conducted in the phytotron with barley (Hordeum vulgare L. cv. Minorimugi) grown in nutrient solution to compare iron (Fe) deficiency caused by the lack of Fe with manganese (Mn)‐induced Fe deficiency. Dark brown spots on older leaves and stems, and interveinal chlorosis on younger leaves were common symptoms of plants grown in either Mn‐toxic or Fe‐deficient treatments. Dry matter yield was affected similarly by Fe deficiency and Mn toxicity. The Mn toxicity significantly decreased the translocation of Fe from roots to shoots, caused root browning, and inhibited Fe absorption. The rate of Fe translocated from roots to shoots in the 25.0 μM Mn (toxic) treatment was similar to the Fe‐deficient treatment. Manganese toxicity, based on the release of phytosiderophore (PS) from roots, decreased from 25.0>250>2.50 uM Mn. The highest release of PS from roots occurred 7 and 14 days after transplanting (DAT) to Mn‐toxic and Fe‐deficient treatments, respectively; but was always higher in the Fe‐deficient treatment than the Mn‐toxic treatments. The release of PS from roots decreased gradually with plant age and with severity of the Mn toxicity symptoms. The PS content in roots followed the PS release pattern.     

Overcoming Fe deficiency in guava (Psidium guajava L.) by co-situs application of controlled release fertilizers

Abstract

Among micronutrient deficiencies, Fe deficiency is the most difficult nutritional disorder to prevent in the fruits of trees growing on calcareous soils. In this study, a pot experiment was carried out to evaluate the potential of co-situs application of controlled release fertilizers (CRF) in alleviating Fe deficiency and improving the growth of fruit trees growing on calcareous soil (pH 9.3). Guava (Psidium guajava L.) seedlings were used as test plants because of their sensitivity to Fe deficiency. Treatments consisted of the following: (1) broadcast application of readily soluble Fe, Zn, Cu, B and Mn fertilizers (Control) or (2) co-situs application of CRF containing N, P, K, Mg, Fe, Zn, B, Cu and Mn (Co-situs). For the Control treatment, CRF containing only N, P and K was used. Both treatments received the same amount of all nutrients. Plants were more chlorotic in young leaves under the Control treatment and the Fe content of young leaves was significantly (least significant difference [LSD_0.05]) higher under the Co-situs treatment. Dry matter production of shoots under the Co-situs treatment was 5.2-fold higher than under the Control treatment, and the total accumulations of macro and micronutrients were much higher under the Co-situs treatment than the Control treatment. Total accumulations of N, P, K, Ca and Mg were 5.0, 4.1, 9.6, 3.2 and 2.2-fold higher, respectively, under the Co-situs treatment compared with the Control treatment, and Fe, Zn, Cu and Mn accumulations were 3.2, 4.1, 6.0 and 3.7-fold higher, respectively. Iron deficiency in guava seedlings was successfully alleviated by the co-situs application of controlled fertilizer, proving the high potential of this method in alleviating Fe deficiency in fruit trees growing on calcareous soils.     

Seasonal Changes of Mineral Nutrients in Fruit and Leaves of ‘Newhall’ and ‘Skagg's Bonanza’ Navel Oranges

In southern Jiangxi province of China, ‘Newhall’(Citrus sinensis Osbeck) navel orange presented a conspicuous symptom of boron (B) deficiency in mature leaves, whereas B deficiency symptoms were not manifested on ‘Skagg's Bonanza’(C. sinensis Osbeck) navel orange. In this study, changes in concentrations of B, calcium (Ca), potassium (K), magnesium (Mg), manganese (Mn), and zinc (Zn) were comparatively investigated in the structural parts of the fruit (rind and pulp) and leaves (old leaves from last season and spring-flush leaves from current year) of ‘Newhall’ and ‘Skagg's Bonanza’ navel orange during the growing season. Two peaks of B concentrations were observed in the rind of the two cultivars during fruitlet growth and fruit enlargement, respectively. Boron concentrations were relatively high in the rind during fruitlet growth, and then decreased in both rind and pulp, whereas, during middle and late fruit enlargement significant increases were found for B in both rind and pulp of the two cultivars. Boron concentrations in old leaves of ‘Newhall’ decreased progressively and remained relatively low, whereas that of ‘Skagg's Bonanza’ was relatively high and changed slightly as the season progresses. Both Ca and K concentrations were above the critical threshold values, while their dynamics were reverse to that of B in fruit and leaves during certain times. Old leaf Mg concentrations of samples at 140 days after full bloom from the two cultivars and spring-flush leaves from ‘Newhall’ were below the threshold limit for sufficiency. In addition, Mg in old leaves was much lower from ‘Newhall’ than from ‘Skagg's Bonanza’. Spring-flush leaf concentrations of Mn and Zn and Mn concentrations in old leaves from ‘Newhall’ were relatively lacking during middle and late season, which accelerated the occurrence of B deficiency symptoms on mature leaves of ‘Newhall.’     

Effects of excess manganese on mineral uptake in mycorrhizal sorghum

Associations between vesicular‐arbuscular mycorrhizal (VAM) fungi and manganese (Mn) nutrition/toxicity are not clear. This study was conducted to determine the effects of excess levels of Mn on mineral nutrient uptake in shoots and roots of mycorrhizal (+VAM) and non‐mycorrhizal (‐VAM) sorghum [Sorghum bicolor (L) Moench, cv. NB9040]. Plants colonized with and without two VAM isolates [Glomus intraradices UT143–2 (UT1 43) and Gl. etunicatum UT316A‐2 (UT316)] were grown in sand irrigated with nutrient solution at pH 4.8 containing 0, 270, 540, and 1080 μM of added Mn (as manganese chloride) above the basal solution (18 μM). Shoot and root dry matter followed the sequence of UT316 > UT143 > ‐VAM, and shoots had greater differences than roots. Shoot and root concentrations and contents of Mn, phosphorus (P), sulfur (S), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), zinc (Zn), and copper (Cu were determined. The +VAM plants generally had higher mineral nutrient concentrations and contents than ‐VAM plants, although ‐VAM plants had higher concentrations and contents of some minerals than +VAM plants at some Mn levels. Plants colonized with UT143 had higher concentrations of shoot P, Ca, Zn, and Cu and higher root Mg, Zn, and Cu than UT316 colonized plants, while UT316 colonized plants had higher shoot and root K concentrations than UT143 colonized plants. These results showed that VAM isolates differ in enhancement of mineral nutrient uptake by sorghum.     

Rootstock Effect on Nutrient Concentration of Sweet Cherry Leaves

The present investigation aimed to study the leaf mineral composition of sweet cherry trees on different rootstocks, since the literature data on uptake efficiency of different rootstocks is inconsistent. Results confirmed the different uptake efficiency of rootstocks. The efficiency of ‘GiSelA 6’ root is emphasized in uptake and supply of leaves with nitrogen (N), phosphorus (P), potassium (K), zinc (Zn), boron (B), and manganese (Mn), but trees on this rootstock tend to develop calcium (Ca), magnesium (Mg), and copper (Cu) deficiencies. The Prunus mahaleb rootstocks on calcareous sandy soil are efficient supplier of N, P, K, Ca, Mg, Fe, and Cu, but this root tends to develop Zn, B, and Mn deficiencies. Prunus avium seedling as rootstock proved to be less efficient in supply of leaves by N, P, K, Ca, and Cu. Prunus fruticosa ‘Prob’ root showed tendency in developing several leaf nutrient deficiencies. The applied fertilizer program led to low nutrient levels or even deficiency symptoms in leaves.     

Preferential translocation of boron to young leaves in Arabidopsis thaliana Regulated by the BOR1 Gene

A mutant of Arabidopsis thaliana, bor1-1 (Noguchi et al. 1997: Plant Physiol., 115, 901–916) requires high levels of boron (B) for normal growth. We analyzed the B-deficiency symptoms of bor1-1 mutant plants in detail. A low B supply retarded the growth of the mutant plants more evidently in leaves than in roots. In particular, cell expansion and formation of air spaces were severely impaired by B deficiency in young rosette leaves. Such defects in growth were correlated with the reduced contents of B in leaves. These defects were not observed when a sufficient amount of B was supplied. Uptake experiments with ¹⁰B-enriched tracer B demonstrated that B taken up through roots was preferentially transported to young leaves compared to old leaves in the wild-type plants under a low B supply. Such a preferential transport to young leaves was not evident in the mutant plants. In conclusion, our data demonstrated that in A. thaliana plants B is preferentially transported to young organs under a low B supply and that this transport process is controlled at least in part by the BOR1 gene.     

铁和不同形态氮素对玉米植株吸收矿质元素及其在体内分布的影响 Ⅱ.对铁、锰、铜、锌等营养元素的影响

用营养液培养方法研究了铁和两种形态氮素对玉米植株吸收铁、锰、铜、锌等微量元素及其在体内分布的影响。结果表明:与硝态氮(NO₃^--N)相比,铵态氮(NH₄⁺-N)显著提高了玉米对铁的吸收,降低了对锰、铜及锌的吸收。供铁也明显提高了植株地上部铁的吸收总量,降低了锰及锌的吸收量,尤其是在供应No₃^--N时这种作用更为明显。在缺铁条件下,NH₄⁺-N处理的玉米新叶中铁的含量明显高于NO₃^--N处理;而新叶、老叶、茎中锰、锌、铜含量以及根中锰、锌含量都明显低于NO₃^--N处理。但使用NH₄⁺-N时,根中铜的含量较高。在供铁条件下,NH₄⁺-N处理的玉米植株四个不同器官中锰和锌的含量显著低于NO₃^--N处理的植株,而铜的含量正好相反。在缺铁条件下,玉米新叶中活性锰、活性锌的含量显著高于供铁处理;与NO₃^--N相比,NH₄⁺-N的供应也显著降低了玉米新叶中活性锰以及活性锌的含量。     

Iron Nutrition of Peanut Enhanced by Mixed Cropping with Maize: Possible Role of Root Morphology and Rhizosphere Microflora

Abstract

Field observations have indicated that Fe deficiency chlorosis symptoms in peanut are more severe and widespread in monoculture than intercropped with maize in calcareous soils of northern China. Here we report a pot experiment that investigated the mechanisms underlying the marked improvement in Fe nutrition of peanut grown in mixture with maize. Iron deficiency chlorosis occurred in the young leaves of peanut in monoculture and was particularly obvious at the flowering stage, while the young leaves of peanut grown in mixture with maize remained green throughout the experiment. The chlorophyll and HCl‐extractable Fe concentrations in young leaves of peanut grown in mixture were much higher than those in monoculture, indicating that maize may have markedly improved the peanut Fe nutrition. Growth in mixture was associated with greatly altered root morphology and microbial populations in the rhizosphere of peanut. Visual observation of peanut roots in monoculture showed that they were larger in diameter and shorter than those in mixture. Moreover, peanut roots in mixture with maize produced more lateral roots and had increased root length compared with plants in monoculture. Peanut grown together with maize showed obvious rhizodermal transfer cells in the subapical root zone, but cells with cell wall ingrowths were poorly developed in peanut in monoculture. Mixed culture resulted in a significantly decreased abundance of bacteria in the rhizosphere of peanut compared with monoculture, and electron microscope observations indicated that this was associated with a thicker mucigel layer on the root surface of peanut in mixture with maize. Several root morphological and rhizosphere microbial factors may thus have contributed to the improvement in Fe nutrition of peanut in mixed culture.     

Effect of magnesium on early taro growth

Abstract

Little research has been conducted on magnesium (Mg) nutrition of taro [Colocasia esculenta (L.) Schott cv. ‘Bun Long']. In this study, we evaluated the effects of varying levels of Mg (0.0, 0.05, 0.1, 0.2, 0.4, and 0.8 mM) on taro plants grown hydroponically for 33 days. Magnesium treatment effects were evaluated for dry matter biomass, leaf area, and N, P, K, Ca, Mg, Na, Mn, Fe, Cu, Zn, and B concentrations of old and young leaves. Dry matter of leaves (young, old, and total), roots, corms, petioles, and total biomass were significantly higher in all plus‐Mg treatments than in the zero‐Mg treatment. These same biomass parameters were not different among treatments with Mg (0.05 to 0.8 mM). Leaf area (young, old, and total) did not differ significantly with varying levels of Mg. A quadratic model described the relationship between Mg levels in leaves and solution Mg (r² = 0.99). Young and old leaf Mg concentrations did not differ. Total leaf Mg concentration ranged from 0.07% to 0.42% for the lowest and highest Mg levels in solution, respectively. Leaf Mg effects on total leaf DM was best fit using segmented regression (r² = 0.95), with a corresponding critical leaf Mg concentration (95% of maximum predicted leaf DM) of 0.14%. No significant interactions were observed between Mg and other mineral nutrients. Critical leaf Mg concentration is based on the vegetative growth stage of taro and could be a key index for taro producers who emphasize vegetable leaf, rather than corm production.     

Pre-bloom leaves of blackcurrant can be used to predict boron and manganese nutrition

The aim of the study was to examine the utilization of mineral analyses of pre-bloom leaves and inflorescences to forecast the nutrition of blackcurrant (Ribes nigrum L.). The study was carried out in Poland in 2 consecutive years on 8 mature ‘Tisel’ blackcurrant plantations planted in coarse- and medium-textured soils that were poor in organic matter. Within each plantation, plants were supplied with nitrogen (N), phosphorus (P) and potassium (K) at low, moderate or high doses for each nutrient. Every year, from the same bushes, the pre-bloom leaves, the inflorescences and the mid-summer leaves (as a standard diagnostic tool) were collected 3–6 days before flowering, when ca. 50% of flowers were open, and at harvest, respectively. No relationships between calcium (Ca) and zinc (Zn) in the studied tissues were significant. The pre-bloom leaf concentrations of N, P, magnesium (Mg), boron (B,) iron (Fe), manganese (Mn) and copper (Cu) were correlated with those in the mid-summer leaves. The relationships between the inflorescence concentrations of P, K, Mg, B, and Mn and those in the mid-summer leaves were also significant. However, only linear correlations between B and Mn in the pre-bloom leaves and the mid-summer leaves were sufficiently strong because the determination coefficients for those relationships were 81% for B and 62% for Mn. The calculated optimal concentrations of B and Mn in the pre-bloom leaves varied from 23 to 37 mg/kg and from 46 to 93 mg/kg, respectively.     

Boron deficiency and toxicity symptoms for several crops as related to tissue boron levels

Boron deficiency symptoms in snap beans (Phaseolus vulgaris L.) showed as general yellowing of tops with slow flowering and pod formation, while toxicity caused reduced growth and burned dark brown older leaves especially on the edges. In radish (Raphanus sativus L.)_f B deficiency resulted in roots which were brown upon cutting and had thick periderm. Even at 4 ppm applied B, no visible B toxicity was noted in radish. The plant tissue B levels of less than 9 to 12 ppm were associated with B deficiency in radish and beans; and greater than 125 ppm with B toxicity in beans. In tomatoes (Lycopersicon esculentum, Mill) B deficiency resulted in reduced growth while B toxicity at 4 ppm applied B caused poor and slow germination. Boron deficiency and toxlcity in tomatoes were related to < 12 and > 172 ppm B, respectively, in tissue. No B deficiency was noted in corn (Zea mays L.) and timothy (Phleum pratense L.). The B toxicity in these two crops appeared as marginal burning and dark brown tips of older leaves and was related to greater than 98 and 176 ppm tissue B in corn and timothy, respectively.     

Gornig,Cleve A.I. und Hanaker,J.W. (Hrsg.): Organic Chemicals in the soil environment Vol. I u. II. zus. 968 S., Preis 24,50 und 26,50 US $. Verlag Marcel Dekker,New York 1972

Investigations on translocation of magnesium (²⁸Mg) in sunflower plants. 1. The translocation and retranslocation of Mg respectively ²⁸Mg in sunflower plants of different age have been investigated. The focus of this investigation was on the influence of the composition of the uptake solution and the age of plants on the uptake of Mg respectively ²⁸Mg through the roots or through the leaves and on Mg translocation (distribution). 2. After sunflower plants were grown in Mg-deficient 1/2-Hoagland solution Mg in the plants were redistributed. The Mg in the roots, stems, cotyledons, primary and secondary leaves was retranslocated to the youngest leaves. 3. In isolated, secondary-rooted sunflower shoots grown in Mg-deficient solution, plant Mg was retranslocated into the new roots and into the youngest shoots. 4. The ²⁸Mg-uptake from the 0,2 mM MgSO₄ solution was usually higher than from the 1/10-Hoagland solution. The composition of the uptake solution had little influence on the distribution of ²⁸Mg. Independent of the age plants had the highest ²⁸Mg content in the young and the lowest in the old leaves. 5. Root uptake of ²⁸Mg resulted in a more uniform distribution in the plant than leaf uptake. The old leaves had a higher ²⁸Mg content by root uptake than by leaf uptake of ²⁸Mg. This is probably influenced by transpiration, in combination with the xylem transport of ²⁸Mg after root uptake, which differs from leaf uptake and translocation in basipetal direction. 6. With increased age of plants, the content of ²⁸Mg/10 gr. fresh weight decreased and the difference in content between the parts of plant was higher. The decrease of Mg content in the oldest leaves was the highest. 7. The results showed that Mg was transportable in the phloem. The magnitude and the direction of Mg transport was determined as primarily through the assimilation stream, which is coupled with Mg transport in plants.     

Einfluß der Stickstoffernährung auf den Gehalt an Chlorophyll und Galaktolipiden alter und junger Rapsblätter (Brassica napus L.)

Effects of nitrogen nutrition on the contents of chlorophyll and galactolipids in young and older leaves of oil-seed rape (Brassica napus L.) The effect of nitrogen nutrition upon chemical characteristics of chloroplasts from young and older leaves of oil-seed rape (Brassica napus L.) were investigated. Nitrogen influenced the chlorophyll content especially of older leaves. As compared to the optimum supply, the values of young leaves were only reduced at the extreme deficiency and the excessive supply. In contrast to the content of chlorophyll the amounts of monogalactosyl diglyceride of both young and older leaves depended strongly on N-supply. Generally, the amounts of digalactosyl diglyceride were not related to leaf-age and N-supply. With the exception of extreme deficiency the contents of linolenic acid in galactolipids were influenced by nitrogen mainly in older leaves. (Brassica napus L., N-supply, Leaf-age, Chlorophyll, Galactolipids)     

Effects of Rhizobium inoculation and magnesium application on growth and nodulation of soybean (Glycine max L.)

Abstract

Magnesium (Mg) deficiency is one of the major nutritional problems in tropic and subtropic areas, where the most soils are acidic. In this study, the effects of Mg application and Bradyrhizobium inoculation on growth, nodulation, symbiotic nitrogen (N) fixation as well as N nutrition status in soybean (Glycine max L.) were investigated in hydroponics under greenhouse conditions. With the increase of Mg up to 0.75?mM at low N and up to 0.5?mM at high N solutions, the dry weights of shoots, roots, and pod grain yield in soybean were increased, while further increase in Mg supply inhibited soybean growth. The availability of Mg was found to entail an improved uptake of N by plants and nodulation process in the root by Bradyrhizobium. Inoculation with rhizobial inoculants not only formed many nodules, but also increased soybean shoot, root biomass and yield, as well as plant N nutrient status.     

NITROGEN ABSORPTION AND RESPIRATION IN WHITE AND BROWN PEACH ROOTS

The relationship between root age and root physiology is poorly understood, despite its importance for nutrient absorption. In peaches, roots are white when they first appear and then become brown with age, which corresponds to a number of physiological changes. We related root browning to nitrogen (N) absorption and respiration in order to provide a better understanding of how color changes as typically observed using minirhizotrons relate to changes in root physiology. The experiment was conducted on peach seedlings (Prunus persica cv. ‘Guardian’) grown in 30-L pots in a greenhouse. Brown roots showed lower respiration rates than white roots. White roots showed a higher ¹⁵N uptake than brown roots and higher concentration of N, potassium (K), magnesium (Mg), manganese (Mn), iron (Fe) and copper (Cu), no significant differences were observed regarding calcium (Ca), and zinc (Zn) concentration.