八达岭山地次生林土壤养分与磷酸酶活性研究

 为探讨北京八达岭山地土壤肥力状况,以黑桦、糠椴、核桃楸3种次生林为对象,对其土壤养分和磷酸酶活性进行研究。结果表明:土壤养分有明显的表聚效应;土壤全磷含量仅为0.39～0.66g.kg-1之间,有可能出现缺磷现象;土壤磷酸酶的活性以酸性磷酸酶为主,呈垂直递减分布规律;各种土壤养分和磷酸酶活性均有明显的根际效应,根系/本土比值在1.02～5.25之间。用SPSS软件进行分析表明,土壤磷酸酶活性与土壤pH、有机质、全氮、有效氮、有效磷和速效钾显著正相关,可以作为土壤肥力评价的一个指标。     

两个水稻基因型根际磷素组分、pH以及磷酸酶活性的动态变化

A rhizobox experiment with two phosphorus （P） treatments, zero-P （0 mg P kg^-1） and plus-P （100 mg P kg^-1） as Ca（H2PO4）2.H2O, was conducted to study the chemical and biochemical properties in the rhizosphere of two rice genotypes （cv. Zhongbu 51 and Pembe） different in P uptake ability and their relationship with the depletion of soil P fractions. Plant P uptake, pH, phosphatase activity, and soil P fractions in the rhizosphere were measured. Both total dry weight and total P uptake of Pembe were significantly （P 〈 0.05） higher than those of Zhongbu 51 in the zero-P and plus-P treatments. Significant depletions of resin-Pi, NaHCO3-Pi, NaHCO3-Po, and NaOH-Pi, where Pi stands for inorganic P and Po for organic P, were observed in the rhizosphere of both Zhongbu 51 and Pembe under both P treatments. Pembe showed a greater ability than Zhongbu 51 in depleting resin-Pi, NaHCO3-Pi, NaHCO3-Po, NaOH-Pi, and NaOH- Po in the rhizosphere. HCl-Pi and residual-P were not depleted in the rhizosphere of both genotypes, regardless of P treatments despite significant acidification in the rhizosphere of Pembe under zero-P treatment. Higher acid phosphatase （AcPME） activity and alkaline phosphatase （AlPME） activity were observed in the rhizosphere of both Zhongbu 51 and Pembe compared to the corresponding controls without plant. AcPME activity was negatively （P 〈 0.01） correlated to NaHCO3-Po concentration in the rhizosphere of both Zhongbu 51 and Pembe, suggesting that AcPME was associated with the mineralization of soil organic P.     

Effects of Soil Drying on Soil pH and Nutrient Extractability

A sample set from a field experiment conducted at two sites, a commercial organic potato farm and a conventionally managed experiment station farm, was used to compare the extractability of nutrients in field-moist and air-dried soils. Standard soil characterization methods of the Maine Soil Testing Service were used to determine soil pH and extractable nutrient content. The data were analyzed with Systat using paired t-tests. Air drying decreased soil pH and increased extractability of calcium, micronutrients, and metals. Many of the observed changes were probably a result of increasing surface acidity with drying. Drying increased the extractability of inorganic phosphorus, probably because of disruption of aluminophosphate complexes, particularly in conventionally managed soils, which had received high amounts of inorganic phosphorus fertilizer. Drying also increased the extractability of complexed phosphorus, probably both organically and inorganically complexed phosphorus, and decreased the extractability of potassium, probably by enhancing potassium fixation in clay interlayers.     

Adopting a Robotic pH Instrument for Soil and Soil-Buffer pH Measurements in a Soil Test Laboratory

Optimum software settings were evaluated to minimize time of analysis without sacrificing data quality on an AS-3010D LabFit robotic instrument. There was little to no difference between soil and soil-buffer pH values measured via manual methods and LabFit analysis with four software settings. A setting for routine laboratory analysis was determined by evaluating r² values from a comparison of LabFit versus manual measurements. Settings for routine analysis were adopted as follows: 5 s for sample delay time, 60 s for maximum sample time, 10 readings for stability, 0.02 for pH stability, 0 s for buffer run delay, and probe rinsing after every 40 samples. These settings were evaluated on 2933 Kentucky soil samples where robotic versus routine analysis of soil and soil-buffer pH resulted in r² values of 0.94 and 0.82 for soil pH and soil-buffer pH, respectively. The results validated the utility of robotic instruments for soil pH analysis.     

The effects of cations and pH on the phosphatase activity of a mixed enzyme system extracted from soil microorganisms

Enzymes extracted from soil microorganisms exhibited activity with a number of inorganic phosphate substrates. Maximum hydrolysis of sodium and ammonium polyphosphates took place at about pH 8.5 in the presence of magnesium or manganese. Optimum cation concentrations were 2 mM for magnesium and 0.8 mM for manganese. Calcium inhibited both magnesium and manganese activation and was itself a non-activator, but an increase in the amounts of magnesium and manganese beyond their optimum concentrations reduced the inhibitory effects of calcium. There was a decrease in the rate of enzymatic hydrolysis as chain length of the polyphosphates increased, but the substrate cation had no effect.     

Soil calcium and pH monitoring sensor system

An agrarian sensorial system based on temperature, moisture, and all solid-state ion-selective potentiometric sensors was developed with the objective of monitoring the behavior of H+ and Ca2+ ions in soil and in real conditions, contributing with a new tool that tries to complement the current precision agriculture technology. The evaluation of the sensorial system to pH monitoring presented a good correlation between the results obtained by the system and the standard methodology, allowing us to notice the soil buffer capacity at different soil depths. With regard to calcium, the sensor system also presented an agreement between its results and those obtained by flame atomic absorption spectrometry, using a calibration model based on multiple linear regressions that allows the correct determination of Ca2+ concentrations in soil depths where the relative moisture is different. In this way, using well-known potentiometric sensors in a complex, discontinued, and heterogeneous matrix, such as soil, the sensorial system proved to be a useful task for agrochemical field applications.     

土壤pH值与土壤因子灰色关联探讨

对于植物生长和发育来说,土壤养分是必要的营养物质,其有效性直接关系着植物发育情况、土壤肥力以及土壤生产力。土壤pH值对养分的有效性影响很大,为了研究其关联性,选用大庆市大同区四乡镇土壤作为样品,对样品的数据结果进行标准化处理,采用灰色关联法对土壤pH值与各土壤养分因子的关联性进行研究。结果表明,与pH值关联度最大的是有效氮,灰关联度为0.928。生产中应采取积极措施改善土壤酸碱度,合理施氮肥,以促进农作物更好地生长。     

苏打盐碱化土壤pH与团聚体中球囊霉素相关土壤蛋白含量的关系

球囊霉素相关土壤蛋白(Glomalin-related soil protein,GRSP)在土壤团聚体形成中起重要作用,与土壤团聚体稳定性正相关。土壤盐碱化破坏土壤结构,降低土壤中GRSP含量,但影响多大尺寸团聚体GRSP含量并不清楚。本文采集45个松嫩盐碱化草地土壤样品,通过干筛法分离出直径0.25、0.25~1和1~2 mm 3种不同粒级团聚体,采用Bradford法测定土壤中GRSP含量,并测定土壤盐碱化指标,经Pearson相关分析和前向选择变量多元线性回归分析,结果显示:土壤pH显著影响各粒级团聚体总球囊霉素相关土壤蛋白(T-GRSP)含量和难提取球囊霉素相关土壤蛋白(DE-GRSP)含量,二者存在显著负相关关系,特别是0.25~1 mm粒级团聚体DE-GRSP含量与土壤pH存在极显著负相关关系,可解释22.3%的DE-GRSP含量变化。土壤pH、电导率、碱解氮和有效磷对各粒级团聚体易提取球囊霉素相关土壤蛋白(EE-GRSP)影响不显著。结果表明土壤苏打盐碱化影响0.25~1 mm团粒结构中较稳定的DE-GRSP含量,可能对土壤团聚体胶联和土壤碳存储产生负影响。     

Assessment of phosphatase activity in soils

In many of the publications concerning the measurement of phosphatase activities in soils, the basic rules governing enzyme assays have, at best, been only partially obeyed.This review article concentrates on the measurement of phosphatase activity, draws attention to the wide discrepancies in its measurement found in the literature and urges the adoption of a more uniform technique obeying the rules governing simple enzyme kinetics.     

毕节烟区土壤pH值分布状况及与土壤养分的关系

分析了贵州省毕节市植烟土壤pH的分布状况及与土壤养分的关系。研究表明,毕节市植烟土壤pH平均值为6.39,变异系数为11.87%,pH值处于最适宜范围(5.5~6.5)的土壤样本数占总样本数的44.83%;各县(市)pH最适宜的土壤样本数占各地总样本的比例由高到低顺序为:金沙(67.44%)>织金(64.29%)>大方(54.84%)>七星关(47.54%)>黔西(36.59%)>威宁(35.19%)>赫章(24.24%)>纳雍(15.38%)。不同土壤类型pH平均值高低顺序为:石灰土>紫色土>潮土>黄壤>黄棕壤;毕节烟区土壤pH与全氮含量呈极显著正相关,与有效硼和有效锌的含量呈显著正相关,而与铵态氮、有效锰、有效铁的含量呈现极显著负相关。毕节市植烟土壤的有机质、全氮、有效硫、铜、锰、铁的含量丰富,但全磷、有效硼、水溶性氯的含量偏低或极低。     

Soil urease activity and kinetics

The activity and kinetic properties of urease in several Malaysian soils were examined. The values for K_m and V_max of the soils computed according to the Hanes equation were in general agreement with other reports as far as magnitudes were concerned. A significant correlation between K_m and V_max was also obtained. The urease activity of the soils was variable, and it was noted that expression of the activity as the time required to hydrolyze half of the applied urea has limited use in soils of low activity. In all soils studied, inhibition of urease activity was effectively achieved using Ag⁺, while Cu²⁺ was only effective in two soils, and marginally effective in the other two soils. Urease inhibitors have potential applications in reducing volatilization losses of ammonia derived from urea applied to soils.     

茶园土壤pH变化对土壤中铝特性的影响

土壤中铝的毒性强弱取决于铝的形态。为了解茶园土壤中铝的特性,以江苏省7个长期定位观测茶园为研究对象,采用化学连续提取法测定不同条件下土样中不同形态的铝含量,研究茶园土壤pH升高或降低过程中铝的形态转化及其影响因素。结果表明,茶园土壤在酸化过程中活性铝溶出明显增强,不同形态的铝含量有明显差异,依次为腐殖酸铝(Alh)铝的水合化物和氢氧化物(Aloh)交换态铝(Alex)有机态铝(Alo)水溶态铝(Alw)无机吸附态铝(Alino);土壤pH、有机质和酸容量是影响铝形态的重要因素;茶园土壤酸化过程中铝的水合化物和氢氧化物、无机吸附态铝以及水溶态铝会转化为交换态铝。土壤pH升高,交换态铝转化成羟基铝;有机结合态铝会影响其他铝形态的转化,腐殖酸铝在土壤pH升高时转化为铝的水合化物和氢氧化物。     

Water-extractable soil organic matter inhibits phosphatase activity

We show that water-extractable soil organic matter may inhibit activity of added phosphatase. The inhibition was greater for the topsoils than the corresponding subsoils. The enzyme kinetics in buffer and in soil solutions followed Michaelis–Menten kinetics. Vmax decreased and Km increased with increasing soil water-extractable organic carbon. Both kinetic parameters followed similar trends with the intensities of both fluorescence peaks of the solutions. The effect was more marked at pH below the optimum pH. Inhibition was complex, particularly for topsoil solutions where Vmax was decreased and Km increased. This effect could lead to underestimation of catalytic activity of enzymes extracted from soil and could result in the overestimation of decreased specific activity in the adsorbed phase of enzymes added to soils.     

Soil pH and magnesium effects on manganese toxicity in peanuts

Manganese (Mn) toxicity can develop in peanuts grown on low pH soils. The objectives of this study were to quantify the impact of soil pH and magnesium (Mg) on the uptake of Mn and the development of Mn toxicity symptoms in peanut plants and to evaluate the use of the Mg:Mn ratio as a diagnostic tool for Mn toxicity in peanuts. Three greenhouse tests were utilized to meet these objectives: a study to determine dolomitic limestone effects, an experiment comparing rate effects of calcium (Ca), Mg, and potassium (K) on Mn toxicity, and a test to separate the effects of pH, Mn, and Mg on Mn toxicity. Soil, leaf, and stem samples were taken for analysis, and toxicity ratings were made. Increasing pH diminished the toxicity rating and leaf Mn concentration and increased the leaf Mg:Mn ratio. The toxicity rating was significantly correlated with both leaf Mn and Mg:Mn ratio, but leaf Mn generally had stronger correlations and was more useful in diagnosis. Magnesium application resulted in a marked reduction in the Mn toxicity rating and leaf and stem Mn concentrations in the second experiment; however, this result was not repeated in the final test. Using Mg to prevent Mn toxicity would require large Mg applications, which could have the serious detrimental effect of interfering with Ca uptake by the peanut fruit. Liming is the more practical method for avoidance of Mn toxicity in peanuts.     

Effects of Soil pH on Wheat Grain Yield and Quality

The effects of planting wheat in permanent beds with fertilization on grain yield and quality need to be better understood. An experiment was conducted at five sites during 2008 and 2009. The objective was to estimate the effects on wheat (Triticum aestivum L.) grain yield and quality of two granular forms of nitrogen (N) (urea and ammonium sulfate, AS) split applied at planting and tillering, and three sprays (urea, AS, and a fungicide) at anthesis. The granular N source affected yield, spike number, and rheological parameters depending upon the soil reaction. Dough resistance/extensibility ratio (P/L) was associated with the normalized difference vegetative index (NDVI) readings collected during tillering before the granular N application. Fungicide spray at anthesis improved yield and grain physical quality evaluated as thousand-grain weight (TGW), test weight, and hardness. Grain protein concentration (GPC) appeared to be mainly affected by environmental factors rather than fertilization practices.     

土壤pH值的测定国际国内方法差异研究

将国际标准化组织于2005年2月15日发布的《土壤质量─pH的测定》(ISO10390:2005)的国际标准方法与国内使用的土壤pH测定方法做对比,研究了不同方法土壤样品制备、悬浊液制备、电极选择、温度和振荡时间等测定条件控制以及测试报告编写几个方面的差异,为分析方法改进作参考。     

北京城区土壤pH分布研究

本文分析北京城区31个样点土壤pH值,讨论北京城区土壤pH值的分布特征和存在的问题。按土壤pH值大小,北京城区土壤pH值呈从中心到四周由低到高,再由高变低,并且呈北向南递减的特点。北京城区土壤pH处于正常范围内,但未来北京城区土壤有发生酸化的可能,有针对性地提出防止土壤酸化的对策。     

蓝莓对土壤酸碱敏感性的量化表征研究

土壤酸碱度是影响蓝莓树体正常生长的重要环境因子之一,量化表征土壤酸碱度变化对保持蓝莓正常生长、提高经济效益的影响幅度至关重要.本文利用Meta分析方法系统地集成国内外87篇文献,共筛选12个蓝莓品种,利用文献中已提取数据组定量化拟合土壤pH变化对不同品种蓝莓树体生长的影响差异.结果表明:根据土壤pH变化对蓝莓树体生长的...     

Soil pH Buffering Capacity and Nitrogen Availability Following Compost Application in a Tropical Acid Soil

Tropical acid soils are highly weathered as they exist under tropical environment with high rainfall and temperature throughout the year, which affects nitrogen availability. Soil organic nitrogen is important in estimating soil nitrogen availability. The combined use of urea and compost in this study was carried out to decrease sole dependence on urea, buffer soil acidification, and reduce nitrogen losses through leaching. Thus, soil buffering capacity, incubation, and organic nitrogen fractionation studies were conducted to determine soil buffering capacity, availability of total nitrogen, organic fractions nitrogen, and inorganic nitrogen in soil after 90 days of incubation following compost. Soil pH, buffering capacity, total nitrogen, organic nitrogen fractions, exchangeable ammonium, and available nitrate were higher in all treatments with compost and combined use of urea and compost. Total hydrolyzable nitrogen, ammonium-nitrogen, (ammonium + amino sugar)-nitrogen, amino sugar-nitrogen, and amino acid-nitrogen were higher in soils with urea and compost suggesting that decomposition of soil organic fractions nitrogen into inorganic nitrogen (ammonium and available nitrate was affected by the addition of urea and compost. Urea can be amended with compost to regulate availability nitrogen in soil for crop use.     

Predicting Liming Needs of Soybean Using Soil pH,Aluminum, and Manganese Soil Tests

Abstract

Whether a tropical soil should be limed or not for a particular crop is strongly dependent on the levels of soil aluminum (Al) which can be determined with soil tests. Soil pH is used to predict whether lime is needed in less‐weathered soils, although some evidence indicates a soil Al test would be more accurate. The objectives of this study were to determine and to compare the accuracies of four soil tests to separate soils requiring lime from those that do not, and to determine the cause of acid‐soil injury to soybean [Glycine max (L.) Merr.]. Soybean was grown in the greenhouse on four surface soils representing the major land resource areas of Louisiana and were amended with eight rates of lime, yields determined, and soils analyzed for soil pH, extractable Al, CaCl₂‐extractable Al, CaCl₂‐extractable manganese (Mn), and Al saturation. Acid‐soil injury in soybean grown on the Litro clay and Stough fsl was probably caused by soil‐Al effects while low soil calcium (Ca) and high soil Mn was likely responsible for lower yields from the Mahan fsl. Leaf Ca from the limed Mahan‐soil treatment was 5‐fold greater and leaf‐Mn 7‐fold less than control levels. Regression analyses’ R² values were similar for all soil tests except for CaCl₂‐extractable Mn, which was lower. Soil tests were compared across soil type by selecting treatments that had the same 85% relative yield. Using this data subset, there was no difference in the soil pH among the four soils, while there were significant differences among soils for all other soil test measurements indicating the superiority of soil pH for identifying acid‐soil injury. Critical test values were 5.1 soil pH, 30 mg kg^‐1 extractable Al, 7% Al saturation, 0.7 mg‐kg^‐1 CaCl₂‐extractable Al, and 9 mg‐kg^‐1 CaCl₂‐extractable Mn.