Response of nitrogen fixation,nodule activities,and growth to potassium supply in water‐stressed broad bean

Three‐week‐old nodulated faba bean plants were subjected to two levels of water stress (0.5 and 0.25 field capacity; soil water content of 20 and 10%) for five weeks. Half of the stressed plants was treated with potassium chloride (KC1) at 10 (K₁) and 150 mg (K₂)/kg soil at the beginning of water deficit. Nodulation was examined and some nodule activities were assayed. Nodulation, nitrogenase activity, total nitrogen (N), and dry matter yield were significantly decreased by increasing stress but were significantly higher with the two levels of potassium (K) supply. Leghaemoglobin and protein contents of cytosol as well as nodule protease and invertase were severely depressed by drought stress. Soluble carbohydrate contents of nodules, however, was significantly increased. Protein and leghaemoglobin contents and enzyme activities were greater with K fertilization but less soluble carbohydrate was accumulated. The results indicate that K supply, particularly at the 150 mg/kg soil level, increased faba bean resistance to water stress.     

Nutrient charge,composition of media and nitrogen‐form affect growth of vinca

Growth of vinca [Catharanthus roseus (L.) G. Don ‘Grape Cooler'] was compared under several cultural conditions. Conditions investigated included two types of media (a peat‐lite mix and a mix containing 25% pine bark) and five types of nutrient charges in the peat‐lite media (sulfated micros, chelated micros, sulfated or chelated micros with pH adjustment to 5.5, and no charge). Nitrogen (N) source effect on growth was also investigated. Plants were grown at five different ratios of nitrate‐N to ammonium‐N. Greatest growth as measured by shoot length and shoot dry weight occurred in the peat‐lite media at either the sulfated micro or chelated micros adjusted to pH 5.5 and at the highest ratios of nitrate‐N to ammonium‐N. Root dry weight and growth were negatively affected by high levels of ammonium‐N in the fertilizer solution.     

Time of availability influences mixed‐nitrogen‐induced increases in growth and yield of wheat 1

Previous studies have indicated that under hydroponic conditions, spring wheat (Triticum aestivum) plants produce higher grain yields, more tillers, and increased dry matter when continuously supplied with mixtures of NO₃ and NH₄ than when supplied with only NO₃. The objective of this study was to determine if mixed N needs to be available before or after flowering, or continuously, in order to elicit increases in growth and yield of wheat. During vegetative development, plants of the cultivar ‘Marshal’ were grown in one of two nutrient solutions containing either a 100/0 or 50/50 mixture of NO₃ to NH₄ and, after flowering, half the plants were switched to the other solution. At physiological maturity, plants were harvested, separated into leaves, stems, roots, and grain and the dry matter and N concentration of each part determined. Yield components and the number of productive tillers were also determined. Availability of mixed N at either growth stage increased grain yield over plants receiving continuous NO₃, but the increase was twice as large when the mixture was present during vegetative growth. When the N mixture was available only during vegetative growth the yield increase was similar to that obtained with continuous mixed N. The yield increases obtained with mixed N were the result of enhanced tillering and the production of more total biomass. Although plants receiving a mixed N treatment accumulated more total N than those grown solely with NO₃, the greatest increase occurred when mixed N was available during vegetative growth. Because availability of mixed N after flowering increased the N concentration over all NO₃ and pre‐flowering mixed N plants, it appears that the additional N accumulation from mixed N needs to be coupled with tiller development in order to enhance grain yields. These results confirm that mixed N nutrition increases yield of wheat and indicate that the most critical growth stage to supply the N mixture to the plant is during vegetative growth.     

Variation of early growth and nutrient content of no‐till corn and soybean in relation to soil phosphorus and potassium supplies

Abstract

Knowledge of relationships between variation in early plant growth and soil nutrient supply is needed for effective site‐specific management of no‐till fields. This study assessed relationships between soil test phosphorus (STP) and potassium (STK) with early plant growth and P or K content of young corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] plants in eight no‐till fields. Composite soil (0–15 cm depth) and plant (V5‐V6 growth stages) samples were collected from 400‐m² areas at the center of 0.14‐ha cells of a 16‐cell square grid and from 2‐m² areas spaced 3 m along each of two 150‐m intersecting transects. Correlation, regression, multivariate factor analyses were used to study the relationships between the variables. Variability was higher for samples collected from the transects. Plant dry weight (DW), P uptake (PU), and K uptake (KU) usually were correlated with STP and STK but the correlations varied markedly among fields. Relationships between soil and plant variables could not always be explained by known nutrient sufficiency levels for grain production. Plant P concentration (PC) was not always correlated with STP and sometimes it increased linearly with STP, but other times increased curvilinearly until a maximum was reached. Plant K concentration (KC) usually was correlated with STK, however, and increased linearly with increasing STK even in fields with above‐optimum STK. The results suggest greater susceptibility of early growth to STP than to STK and greater plant capacity to accumulate K compared with P over a wide range of soil nutrient supplies. Variation in STK likely is a major direct cause of variation in KC over a wide range of conditions but variation in STP is not likely a major direct cause of variation in PC when high STP predominates.     

Glutamine synthetase of roots and leaves in response to nitrogen application at different growth stages in field‐grown rice

Information on glutamine synthetase (GS) activity of roots and leaves in response to nitrogen (N) application at different growth stages is limited for field‐grown rice. Root and leaf GS activity was measured on field‐grown rice plants to compare the effects of fertilizer‐N application at midtillering, panicle initiation, and flowering. Leaf GS activity was greater than root GS activity, regardless of N application. Root and leaf GS activity generally declined as plant aged, and the decline was greater in roots than leaves. Leaf and root GS activity were significantly increased by urea‐N applied at different growth stages. Root GS activity was maximum three days after N application, while leaf GS activity peaked one day after N application. Root GS activity showed greater response to N application at midtillering and panicle initiation than leaf GS activity, but the opposite was found at flowering. The stimulation of GS activity by N was greatest at midtillering and reduced with plant age.     

Distribution profiles of dry matter and total nitrogen in leaves and stems of oriental field‐grown tobacco plants at different nitrogen levels

Oriental tobacco plants (Nicotiana tabacum L. cv Myrodata Agrinion) were grown without nitrogen (N) fertilization (N₀) and with added ammonium nitrate at a rate of 50 kg‐ha^‐1 (N₁) and 100 kg‐ha^‐1 (N₂). Non‐uniform patterns for leaf FW and DW changes per node showed a decreasing trend from lower to upper nodes during the vegetative stage. From approaching flowering to fruit set, these patterns became more uniform. Plants which were fertilized with N had increased leaf FW and DW accumulation levels and non‐uniform distribution patterns, primarily during the reproductive stage, and leaves of the lower nodes were found in the older plants. By contrast, the median values of leaf FW for the unfertilized plants were reduced during the reproductive period. The DW/FW×100 ratio values revealed a stable relationship between leaf FW and DW from the vegetative to the reproductive stage, while modified patterns of DW/FW×100 appeared later in the plant cycle. Nitrogen fertilization resulted in an early appearance of modified patterns of DW/FW×100 in the plant life cycle and higher accumulation of dry matter per unit leaf area. Patterns of total leaf N concentration showed an increasing trend from the lower to the upper nodes for all plant ages and treatments. Total N concentration values varied from 1.6%, 1.9%, and 1.8% on a dry matter basis, for the lower node up to 5.5%, 6.3%, and 6.1% for the upper node in young tobacco plants in the N _o , N₁, and N ₂ treatments, respectively. After fruit set, a more uniform distribution of total leaf N was observed among the leaves in all treatments. Concentration values for total leaf N in older plants varied from 1.9%, 2.1%, and 2.2% for the lower node up to 3.4%, 3.3% and 3.2% for the upper node in the N ₀ , N₁, and N₂ treatments, respectively. These results suggest a progressive decrease with plant age for total leaf N concentration in the plant as a whole. The increased N fertilizer level affected the total N level in young plants but not in the older ones. Inflorescence and fruit set periods are critical for plant N balance except for the plants which received the increased N fertilization. The determined total stem N concentration was less than that for the leaves. This change in the stem, similar to leaves, showed an increasing trend from the basal to the upper part and a decreasing trend from the vegetative to the reproductive stage. The total stem N level declined from 1.0–1.2%, 1.6–1.7%, and 2.2–2.9% on a dry matter basis to 0.5–0.6%, 1.0–1.2%, and 1.2–1.6% for the basal, middle, and upper part of the stem, respectively.     

Effect of nitrogen on the growth and photo‐synthetic activity of salt‐stressed barley

Pot experiments were conducted in the greenhouse to study the effect of nitrogen (N) nutrition on photosynthesis and water relations of barley plants under salinity conditions. Nitrogen decreased the sodium (Na) content and increased the potassium (K) content in shoots. The net photosynthetic rate of leaves increased significantly with added N increasing from 0 to 100 mg N/kg soil. The activity of ribulose 1,5 bisphosphate carboxylase (RuBPC_ase) in leaves of high‐salt plants was lower, and in leaves of the low‐salt plants higher than that in control plants. The photosynthetic rate was reduced by sodium chloride (NaCl) and was significantly correlated with total soluble protein per unit leaf area. At each N level, stomatal conductance in leaves was reduced considerably by salt. Proline content of leaves increased with increasing N level. It was higher in leaves of salt‐treated plants than in those of control plants. The osmotic potential of leaves decreased with increasing N applied, and the turgor pressure of high N plants remained higher under salt treatment condition.     

Effect of nitrogen form ratio on pansy growth and nutrition and the palatability to white‐tailed deer

Abstract

Pansies are one of the most popular annual bedding plants in the United States. Growth and uptake of essential nutrients as influenced by N‐form ratio was evaluated in pansy as well as what role pansy nutrition plays in the protection of pansy against feeding damage by white‐tailed deer provided by selected repellents. Plants were grown under three N‐form ratios: 100:0, 50:50, and 0:100NO₃:NH₄. Dry weight was highest for pansy treated with 100:0 and lowest for plants treated with 0:100 NO₃:NH₄ Mean quality ratings were 4.07 for pansies grown with 100:0,1.80 for pansies grown with 50:50, and 0.78 for pansies grown with 0:100. Potassium (K), magnesium (Mg), and manganese (Mn) content was lower in plants treated with 0:100 than in those treated with 100:0. Ammonium may have competed with these cations for uptake. Boron (B), copper (Cu), molybdenum (Mo), and aluminum (Al) content was highest in plants treated with N‐NH₄. There was no significant difference shown in nutrient levels caused by repellent treatments. Feeding damage was shown to be affected by N‐form ratio only on Day 3 of the study. Of the three repellent treatments [Deer and Rabbit Repellent (Thiram), Deer Away purrescent egg spray, and no spray], Thiram provided the greatest protection to pansies over the study period.     

No‐tillage corn response to placement of fertilizer nitrogen,phosphorus, and potassium

Abstract

Fertilizer placement for corn (Zea mays L.) has been a major concern for no‐tillage production systems. This 3‐yr study (1994 to 1996) evaluated fertilizer phosphorus (P) or potassium (K) rates and placement for no‐tillage corn on farmers’ fields. There were two sites for each experiment involving fertilizer P or K. Treatments consisted ofthe following fertilizer rates: 0,19,and 39 kg P ha^‐1 or 0, 51, and 102 kg K ha^‐I. The fertilizer was broadcast or added as a subsurface band 5 cm beside and 5 cm below the seed at planting. Early plant growth, nutrient concentrations, and grain yields were measured. At the initiation of the study, soil test levels for P and K at the 0–1 5 cm depths ranged from optimum (medium) to very high across sites. Effects of added fertilizer and placement on early plant growth and nutrient concentrations were inconsistent. Added fertilizer had a significant effect on grain yields in two of twelve site‐years. Therefore, on no‐tillage soils with high fertility, nutrient addition, and placement affected early plant growth and nutrient utilization, but had limited effect on grain yield. Consequently, crop responses to the additions of single element P or K fertilizers under no‐tillage practices and high testing soils may not result in grain yield advantages for corn producers in the Northern cornbelt regardless of placement method.     

Effect of peduncle‐perfused nitrogen,sucrose, and growth regulators on barley and wheat amino acid composition

Abstract

Nitrogen (N) or growth regulator application to small grain cereals near anthesis has been demonstrated to alter grain fill and grain yield, the protein yield and nutritional quality may also be modified by these management factors. The objective of this study was to determine whether delivery of N, growth regulator, or sucrose solutions into greenhouse‐grown barley (Hordeurn vulgare L. cv. Leger) or wheat (Triticum aestivum L. cv. Katepwa), plants through peduncle perfusion altered the amino acid composition of the grain. The following treatments were tested: N (25 and 50 mM), chlormequat (30 μM), ethephon (15 μM), N + chlormequat, N + ethephon, detillering + N, sucrose (250 mM), distilled water check, and non‐perfused check. Perfusion lasted 30 d beginning 5 to 8 d after spike emergence. Addition of N via peduncle perfusion increased protein concentrations and concentrations of all amino acids in both barley and wheat when expressed in terms of grain dry matter. Protein yield and lysine content were also increased. However, the increase in essential amino acids such as lysine, methionine, threonine, isoleucine, arginine, and leucine was relatively small, and the proportions of these amino acids in the grain protein were actually reduced. The sucrose treatment only affected wheat, increasing lysine concentration and decreasing the total protein concentration. Growth regulators used in this study did not alter protein yield or amino acid composition in either crop.     

The influence of solid urban waste compost and nitrogen‐mineral fertilizer on growth and productivity in potatoes

Abstract

The influence of solid urban waste (SUW) compost and nitrogen (N)‐mineral fertilizer on the growth and productivity in semi‐early harvest potatoes (Solanum tuberosum L, var. ‘Edzina') was studied over a period of three years. Nine treatments derived from a factorial combination of 3 levels of SUW compost (0, 18, 36 T#lbha^‐1) and 3 levels of N‐mineral fertilizer (0, 125, 250 Kg N ha^‐1) were carried out on a sandy, moderately fertile plot. The applications of SUW compost and N‐mineral fertilizer showed a stimulation in plant development with respect to the non‐treated controls. The mineral fertilizer treatment gave markedly higher results. Plant response to the combined mineral fertilizer‐compost treatments demonstrated a tendency towards saturation within each level of application. Potato productivity results indicated that this crop reacts strongly and positively to an application of N‐mineral fertilizer, but only slightly to the treatment levels of compost considered in the study. As a consequence of the application of SUW compost, the application‐response graphs reveal a reduction in the optimal levels of N‐mineral fertilizer application and an increase in the efficacy of the mineral fertilizer.     

Photosynthesis acclimation,leaf nitrogen concentration,and growth of four tree species over 3 years in response to elevated carbon dioxide and nitrogen treatment in subtropical China

Purpose  

Up to date, most studies about the plant photosynthetic acclimation responses to elevated carbon dioxide (CO₂) concentration have been performed in temperate areas, which are often N limited under natural conditions and with low ambient N deposition. It is unclear whether photosynthetic downregulation is alleviated with increased N availability, for example, from increased N deposition due to fossil fuel combustion in the tropics and subtropics. Awareness of plant photosynthetic responses to elevated CO₂ concentration will contribute to the better understanding and prediction of future forest productivity under global change.     

Mehlich‐1‐ and DTPA‐extractable lead in soils in relation to soil properties

Abstract

Mehlich‐1 and DTPA extractants are frequently used to predict metal availability in soils. Metal extractability by the acid or chelate extractant reflects the metal characteristics and metal‐soil interactions. In this study, samples of eight topsoils from the southeastern United States were incubated with added lead (Pb) at the rate of 40 mg#lbkg^‐1. After five months in the greenhouse, Mehlich‐1 and DTPA extractants were employed to extract Pb in both metal‐amended and natural soils. For the natural soils, Pb concentration in the DTPA extractant was always higher than that in the Mehlich‐1 extractant. This indicates that the DTPA chelate extractant is able to dissolve some Pb in soils which is not solubilized by protons. The negative correlation found between Mehlich‐1‐extractable Pb and soil clay content might result from two mechanisms: i) strong association between Pb and soil surfaces, or ii) readsorption of Pb during extraction. None of the correlations between DTPA‐extractable Pb and soil properties was significant, suggesting that the DTPA‐extractable Pb is not heavily dependent on soil properties. The DTPA extractant showed a high ability to solubilize Pb in the natural soils possibly due to a high affinity of Pb for soil organic matter.     

Response of summer‐planted potatoes to level of applied nitrogen and water

The irrigation and nitrogen (N) requirements of potatoes (cv. Delaware) were determined using sprinklers in a line‐source design on a Spearwood sand. Irrigation water was applied at 73 to 244% of the daily pan evaporation (Epan) and N at 0 to 800 kg N ha^‐1 (total applied) as NH₄NO₃ in 10 applications post‐planting. There was a significant yield (total and marketable) response to irrigation, at all levels of applied N, and N at all levels of applied water (P<0.001). The interaction between irrigation and N was also significant (P<0.001). There was no significant yield response to irrigation from 149% Epan (i.e., W3 treatment) to 244% Epan (i.e., W6 treatment). Irrigation at 125 and 150% of Epan was required for 95 and 99% of maximum yield, respectively, as determined from fitted Mitscherlich relationships. Critical levels of N required for 95 (417 kg ha^‐1) and 99% (703 kg ha^‐1) of maximum yield were also determined from a Mitschlerlich relationship fitted to the average of the W3 to W6 treatments. The percent total N and nitrate‐N in petioles of youngest fully expanded leaves required for 95 and 99% of maximum yield was 1.78 and 2.11, respectively, at the 10 mm tuber stage, and 0.25 and 0.80% at the 10mm plus 14 day stage (from quadratic regressions). There was a significant (P≤0.001) increase in N uptake by tubers with level of applied N from 57 kg ha^‐1 at 0 kg applied N ha^‐1 to 190 kg ha^‐1 at 800 kg applied N ha^‐1 (from a Mitscherlich relationship fitted to the average of W3 to W6 treatments). After accounting for N uptake from soil reserves (57 kg N ha^‐1), apparent recovery efficiency (RE) of fertilizer N by tubers [RE=(Up‐Uo/Np) where Up=uptake of N by the crop, Uo=uptake in absence of applied N and Np is the level of applied N, expressed as a fraction] declined from 0.28 at 100 kg applied N ha^‐1 to 0.17 at 800 kg applied N ha^‐1. There was a linear increase in ‘after cooking darkening’ (i.e., greying) of tubers with increasing level of applied N. Conversely, ‘sloughing’ (i.e., disintegration) of tubers decreased (inverse polynomial) with increasing level of applied N. Rate of irrigation had no effect on these cooking qualities. Reducing applied irrigation and N from levels required for 99% of maximum yield to levels required for 95% of maximum yield would not lead to a significant reduction in profit. This would increase apparent recovery efficiency of applied N by plants, maintain tuber quality, and reduce the impact of potato production on the water systems of the Swan coastal plain.     

Nitrate versus chloride nutrition effects in a soil‐plant system on the growth,nitrate accumulation,and nitrogen,potassium, sodium,calcium, and chloride content of carrot

The experiment was conducted to evaluate the effects of various nitrate/ chloride (NO₃/Cl) ratios on growth, nitrate accumulation, and mineral absorption in carrot, Daucus carota L., plants in a controlled environment. The experiment included two Cl sources [potassium chloride (KC1) and calcium chloride (CaCl₂)] and five NO/Clratios at 100/0, 90/10, 80/20, 70/30, and 60/40 with total‐nitrogen (N) concentration of 400 mg NO₃ kg^‐1 soil in 100/0 treatment. Fresh and dry weights of shoots and storage roots, and length and diameter of storage roots increased significantly with mixed NO₃/C1 treatments with both Cl sources as compared to single NO₃ (100/0) treatment. Growth was enhanced up to the 80/20 NO₃/C1 treatments. With Cl present in the treatments, the concentration of total‐N unchanged and NO₃ decreased in plants, and Cl and potassium (K) increased with the Cl sources. In KC1 treatments, Na absorption decreased. Calcium (Ca) content of the plants significantly differed by the treatments. It was concluded that N fertilization provided with combined Cl forms and NO₃/Cl rates can enhance production of better quality carrot and at the same time decrease of the N fertilizer input.     

Influence of Azospirillum inoculation and nitrogen supply on grain yield,and carbon‐ and nitrogen‐assimilating enzymes in maize

Nitrogen (N) supply increased yield, leaf % N at 10 days after silking (DAS) and at harvesting, the contents of ribulose‐1,5‐bisphosphate carboxylase (RUBISCO) and soluble protein, and the activities of phosphoenolpyruvate carboxylase (PEPC), and ferredoxin‐glutamate synthase (Fd‐GOGAT), but not of glutamine synthetase (GS) for six tropical maize (Zea mays L) cultivars. Compared to plants fertilized with 10 kg N/ha, plants inoculated with a mixture of Azospirillum sp. (strains Sp 82, Sp 242, and Sp Eng‐501) had increased grain % protein, and leaf % N at 10 DAS and at harvest, but not grain yield. Compared to plants fertilized with either 60 or 180 kg N/ha, Azospirillum‐inoculated plants yielded significantly less, and except for GS activity, which was not influenced by N supply, had lower values for leaf % N at 10 DAS and at harvest, for contents of soluble protein and RUBISCO, and for the activities of PEPC and Fd‐GOGAT. Yield was positively correlated to leaf % N both at 10 DAS and at harvest, to the contents of soluble protein and RUBISCO, and to the activities of PEPC and Fd‐GOGAT, but not of GS, when RUBISCO contents and enzyme activities were calculated per g fresh weight/min. However, when enzyme contents and enzyme activities were expressed per mg soluble protein/min, yield was correlated positively to RUBISCO and PEPC, but negatively to GS. These results give support to the hypothesis that RUBISCO, Fd‐GOGAT, and PEPC may be used as biochemical markers for the development of genotypes with enhanced photosynthetic capacity and yield potential.     

Fruit growth and mineral element accumulation in pacific rose™ apple in relation to orchard management factors and calyx‐end splitting

Abstract

The effect of irrigation and crop load of apple (Malus domestica Borkh cv. Pacific Rose) on the fruit growth and mineral element accumulation was investigated. Fruit growth and changes in the concentration and contents of nitrogen (N), phosphorus (P), calcium (Ca), magnesium (Mg), and potassium (K) in the flesh of Pacific Rose? apple fruit were recorded over a growing season at the Massey University Fruit Crops Units orchard in the 1998–99 crop season. Fresh mass showed a linear growth while fruit diameter followed a curvilinear growth pattern during the growing season. Low crop load significantly increased fruit size, particularly during the final expansion phase, whereas irrigation had little effect on this attribute. Both low crop load and frequent irrigation treatments increased fruit growth rate, but the effect of the irrigation treatments fluctuated considerably during the season. The incidence of frait splitting was detected at about 20 WAFB when the fruit attained an asymmetrical growth in shape (L/D). The concentrations of N, P, Ca, Mg, and K declined throughout the sampling period. There was however, an increase in concentrations of P and K at the last harvest. The quantity of individual nutrient elements accumulated by the fruit showed an increasing trend during the season. Nitrogen accumulation however, reached maximum at 16 WAFB after which it declined until the last harvest. Fruit mineral element analysis of sound and split fruit revealed that split fruit had higher concentrations and contents of Mg and K and lower contents of Ca and P. As a result, the ratios of Ca: Mg and Ca: K were lower in split fruit as compared with sound fruit.     

Pattern of iron distribution in the soil‐plant system and its possible relation to iron‐chlorosis

Abstract

The frequent concentration‐ranges of various nutrient elements in soils and in plants are compared. Iron is different from almost all other nutrient elements in the fact that its optimal concentration range in plants is much lower than its frequent concentration range in soils. It is suggested that this observation is related to a chemical‐physiological mechanism of control on the uptake of iron by plants which in turn may explain the situations in which iron deficiency conditions in plants arise.     

Effects of supplemental nitrogen on nitrogen‐assimilation enzymes,free amino nitrogen,soluble sugars,and crude protein of rice

Abstract

An upland rice variety IAC‐47 was grown in a greenhouse to determine the effect of foliar nitrogen (N) supplementation during grain development on the activity of the N assimilation enzymes, nitrate reductase (NR) and glutamine synthetase (GS), on free amino‐N content and leaf soluble sugars, and on grain crude protein content. At 10 and 20 days after anthesis (DAA), the leaves were fertilized with a liquid fertilizer containing 32% N as 12.8% urea, 9.6% ammonium (NH₄), and 9.6% nitrate (NO₃) in increasing rates corresponding to 0,20+20, 40+40, and 60+60 kg N ha^‐1. Leaves were collected twice (at 12 DAA and 14 DAA for GS activity, sugar and amino‐N content, and at 11 and 13 DAA for NRA) after each application of leaf N. The late foliar application of N increased significantly grain crude protein without a corresponding decrease in grain weight. The NR activity (NRA) increased after the foliar application of N. In the flag leaf, 60+60 kg N ha^‐1 (21 DAA) resulted in higher NRA (20x over the control), while GS activity was smaller than the control. At 22 DAA there was an increase in GS activity in the flag leaf at 20+20 N level. However, the GS activity decreased as applied N levels increased. Also at the 20+20 level, there were increases in free amino‐N in the flag leaf and second leaf at the final harvest. Throughout the experiment, plants at the 60+60 N level had the lowest levels of soluble sugars. Increases in crude protein were highest at 40+40 N level (27.9%), followed by 60+60 (18.7%).     

Relationships between nitrogen rate,plant nitrogen concentration,yield and residual soil nitrate‐nitrogen in silage corn

Abstract

Nitrogen (N) fertilizer is a key factor of yield increase but also an environmental pollution hazard. The sustainable agriculture system should have an acceptable level of productivity and profitability and an adequate environmental protection. The objectives of this study were to determine the relationships between N rate, DM yield, plant N concentration (NC) and residual soil nitrate‐nitrogen in order to improve the predicted N rate in corn (Zea mays L.) silage. The experiment was conducted over a period of three years in the province of Quebec on three soil series in a continuous corn crop sequence. Treatments consisted of six rates of N: O, 40, 80, 120, 160, and 200 kg N ha^‐1 as ammonium nitrate applied at planting: broadcast and side banded. Four optimum N rates were calculated using different models: (i) economic rate base on fertilizer and corn price using the quadratic model (E); (ii) economic rate based on fertilizer and corn price using the quadratic‐plus‐plateau model (QP); (iii) critical rate based on linear‐plus‐plateau model (P); (iv) lower than maximum rate (L) corresponding to 95% of maximum yield. The optimum plant NC at all growing stages and the N uptake at harvest were calculated depending on these N rates and yields.

The NC of whole plant at 8‐leaf stage (25–30 cm plant height) of ear leaf at tasselling and of whole plant at harvest stage, the N rate, the N uptake at harvest and the DM yield were all significantly intercorrelated and affected by soils and years, but not affected by N fertilizer application method. The DM yield was linearly and significantly related to NC of whole plant at 8‐leaf stage (rv = 0.932**). At this stage, the average NC corresponding to the optimum N rate and yield was of 3.71, 3.68, and 3.66% as calculated with E, L, and P model, respectively. Our data suggest that the NC of whole plant at 8‐leaf stage may be used to evaluate the N nutrition status of plant and the required optimum N fertilizer rate. The NC of ear leaf at tassel stage was also significantly correlated to corn yield (r = 0.994**). It may be used as an indicator to evaluate the near‐optimum N rate in the subsequent years.

The N uptake by whole above‐ground plant at harvest was quadratically related to corn yield. Data show that at high fertilizer N rate, the N uptake still increased without significantly increasing yield. The N uptake was of 176.5, 163.0, and 155.0 kg N ha^‐1 using the E, L and P rates of 146, 126, and 115 kg N applied ha^‐1, respectively. The optimum N rate and yield were affected by soil type and year, but not by the method of N fertilizer application. The yield increased rapidly up to a N rate of about 120 kg N ha^‐1 and then quite slightly to a maximum N rate of 192 kg N ha^‐1. The optimum N rate was of 115 and 126 kg N ha^‐1 using the P and L model respectively and as high as 146.8 kg N ha^‐1 using the E model. The L model, using a much smaller N rate, gave a reasonably high yield compared to E rate (12.2 and 12.5 Mg ha^‐1, respectively). The data show that a relatively much lower N rate than maximum did not proportionally diminish the yield. Thus, for a difference of 40.4% between maximum N rate and P rate a difference of only 7.4% in yield was observed. Using the L model the differences in rate and yield were of 34.4% and 4.7%, respectively. The QP model gave no significant difference compared to E model.

At harvest the residual soil NO₃‐N increased significantly with increasing N fertilizer rate in whole of the 100 cm soil profile, but mainly in the top 40 cm soil layer. The total NO₃‐N found in 0–100 cm profile at rate of 0, 120 and 200 kg applied N ha^‐1 at planting was as high as 33.7, 60.5, and 74.5 kg N ha^‐1 respectively in a light soil and 37.5, 97.5, and 145.5 kg N ha^‐1 in a heavy clay soil. The difference in NO₃‐N content in the 60–100 cm layer between different applied N rate suggests that at harvest, part of fertilizer N applied at planting was already leached below the 100 cm soil layer. Results, thus, show that reasonably high corn yields can be obtained using more adequate N fertilizer rates which avoid the overfertilization and are likely to reduce the air and ground water pollution.