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1.
Abstract

Fertilization and harvest frequency affect yield and quality of forages. The purposes of this experiment were to determine (i) the effects of fertilization and frequent harvesting on yield and quality of tall fescue (Festuca arundinacea Schreb.) and smooth bromegrass (Bromus inermis Leyss.) and (ii) the efficiency of N in animal waste as compared with inorganic N fertilizer for forage production of these cool season grasses. ‘Fawn’ tall fescue and ‘Southland’ smooth bromegrass were grown in the greenhouse on Pullman clay loam topsoil (fine, mixed thermic Torrertic Paleustoll) under eleven fertilizer treatments and two harvest regimes. Nitrogen fertilizer increased yields, N and K concentrations and K/(Ca + Mg) ratios and decreased P, Ca, and Mg concentrations. Phosphorus and K fertilizers did not affect yields but applied P increased P and tended to decrease N and Ca concentrations. Applied K tended to increase Ca concentrations. Recovery of N from feedlot manure ranged from 0.8 to 14%, whereas, recovery from NH4NO3 averaged 64%. Harvesting at 3‐week rather than at 6‐week intervals reduced yields 25%; however, N and P removal were higher under the 3‐week harvest regime. Even though forage production was reduced under heavy utilization, the grasses required more N fertilizer under heavy than under lighter utilization. The two grasses produced similar yields under the 3‐week cutting regime and at N rates through 340 kg/ha under the 6‐week cutting regime. Tall fescue yields were higher with the higher N rates under the 6‐week cutting regime. Smooth bromegrass forage was higher than tall fescue forage in N, K, and Ca, whereas tall fescue forage was higher in P and Mg.  相似文献   

2.
Abstract

In semi‐arid regions, soil depth influences soil N uptake, but not ferilizer N uptake. How soil depth interacts with soil and fertilzer N to influence N uptake in humid regions is not known. The objective was to determine the relative importance of soil depth and soil and fertilizer N uptake, by forage grasses. Tall fescue (Festuca arundinacea Schreb.) and switchgrass (Panicum virgatum L.) were grown on soils of varying depths. Nitrogen rates are 0, 90, and 180 kgN/ha of 15N depleted (NH4)SO4 applied in a split application on fescue and in one aplication to switchgrass. Total N and fertilizer N uptake, were regressed against fertilizer N, variables related to soil depth (waterholding capacity (WC), water use (WU), water loss (WL), and total soil N (SN). Soil variables explained 28% of the accoutable variation in total N uptake by first cut fescue but only 10% by second cut fescue. Soil variables explained 11% of the accountable variation in fertilizer N uptake by first cut fescue and none by the seoond. Soil variables explained 40% of the accountable variation in the total N uptake, by switchgrass, but only 10% of the variation in the fertilizer N uptake. Only where soil depth was less than 90 cm did it have a significant effect on the fertilizer N uptake by first cut fescue. Soil depth had no significant effect on the uptake, of fertilizer N by second cut fescue or switchgrass.  相似文献   

3.
Abstract

Analytical procedures using gas chromatography–ion trap tandem mass spectrometry (GC‐MS/MS) were developed to analyze atrazine (ATR) and its dealkylated metabolites in four forage species (switchgrass, tall fescue, smooth bromegrass, and orchardgrass). Atrazine, deethylatrazine (DEA), and deisopropylatrazine (DIA) were extracted with methanol (CH3OH) followed by liquid–liquid extraction and partitioning into chloroform, with additional cleanup by C18 solid‐phase extraction (SPE). Through the optimization of ionization conditions and ion storage voltages, the background noise of product ion spectra (MS/MS) was reduced dramatically, providing sub‐µg/kg detection limits. Mean recoveries of ATR, DEA, and DIA were 94.3, 105.6, and 113.1%, respectively. The estimated limit of detection (LOD) was 0.6 µg/kg for ATR, 1.3 µg/kg for DEA, and 0.3 µg/kg for DIA. These LODs were one to two orders of magnitude lower than those reported for other GC‐MS, GC‐MS/MS, high pressure liquid chromatography (HPLC)‐UV, or HPLC‐MS/MS procedures designed for food‐safety monitoring purposes. To validate the developed method, a field experiment was carried out utilizing three replications of four forage treatments (orchardgrass, tall fescue, smooth bromegrass, and switchgrass). Forage plants were sampled for analyses 25 days after atrazine application. DEA concentrations in C3 grasses ranged from 47 to 96 µg/kg, about 10‐fold higher than in switchgrass, a C4 species. The ATR and DIA concentrations were similar, ranging from 1.5 to 13.2 µg/kg. The developed method provided sufficient sensitivity to determine the fate of ATR and its chlorinated metabolites via plant uptake from soil or dealkylation within living forage grasses. It also represented significant improvements in sensitivity compared to previous GC methods.  相似文献   

4.
Infection with Neotyphodium spp. endophytes increases resistance to drought stress and soil mineral imbalances in tall fescue (Festuca arundinacea Schreb. = Lolium arundinaceum (Schreb.) S. J. Darbysh.) and meadow fescue (Festuca pratensis Huds. = Lolium pratense (Huds.) Darbysh.). We hypothesized that resistance of these grasses to salinity stress may also be attributed to endophyte infection. Two tall fescue genotypes, Fa75 and Fa83, and one meadow fescue genotype, Fp60, infected (E+) with their endophytic fungi, Neotyphodium coenophialum (Glenn, Bacon and Hanlin) and N. uncinatum (Glenn, Bacon and Hanlin), respectively, and their noninfected counterparts (E–) were cultured in nutrient solution at three salinity levels of 0, 85, and 170 mM NaCl. Except for genotype Fa75, E+ plants exhibited higher leaf survival rates than E– clones at a high salinity level (170 mM). Root dry matter was higher in E+ than in E– plants, but shoot dry matter was not affected by endophyte infection. This resulted in a lower shoot‐to‐root ratio in E+ plants (1.63) compared with E– plants (2.40). Sodium (Na+) and chloride (Cl) concentrations were greater in roots of E– than in E+ clones. In shoots, Na+ and Cl concentrations were not affected by the endophyte. In contrast, E+ plants accumulated more potassium (K+), which resulted in a greater K+ : Na+ ratio in shoots of E+ than in those of E– plants. Our results show that endophyte infection reduced Na+ and Cl concentrations in tall fescue and meadow fescue roots but increased K+ concentrations in the shoots. Based on these results, we conclude that endophyte‐infected grasses may thrive better in salinity‐stress environments.  相似文献   

5.
Abstract

The 2M potassium chloride (KCl) extraction method used to measure soil nitrate (NO3 ‐N) concentrations in soils may introduce some artifacts caused by soil sampling, processing, and handling. Furthermore, this method provides soil NO3 ‐N concentrations for soil sampled at a particular time, whereas the dynamics of this anion in situ need to be better understood. In order to develop a reliable in situ method as an alternative, an anion exchange membrane (AEM) was tested for its ability to adsorb NO3 ‐N from a soil cropped to corn (Zea mays L.) and amended with manure or inorganic nitrogen (N). In a field study, we compared the amount of NO3 ‐N adsorbed on an AEM and extracted with the 2M KCl method. The AEM was calibrated in the laboratory and placed at 15‐cm soil depth for 2‐wk periods during the corn growing season. Nitrate adsorption on the AEM and KCl‐extractable NO3 ‐N were larger in the inorganic N treatment than in the manure or the control treatments throughout the growing season. The NO3 ‐N concentrations measured by the AEM method were correlated with NO3 ‐N extracted with 2M KCl (r2 = 0.78***), suggesting that the AEM method could be used to measure NO3 ‐N concentrations in agricultural soils.  相似文献   

6.
Abstract

Significant losses of nitrogen (N) can occur via volatilization of ammonia (NH3) when non‐incorporated broadcast applications of urea or urea‐containing fertilizers are made. This study was conducted to determine the efficacy of urea nitricphosphate (UNP) as an N and phosphorus (P) source for cool‐season grasses and to evaluate NH3 volatilization potential of UNP as compared to urea under laboratory conditions. A three‐year field study compared UNP to ammonium nitrate (AN) and urea at 56 and 112 kg N/ha for tall fescue (Festuca arundinacea Schreb.) and smooth brome (Bromus inermis Leyss.). Brome yields were significantly higher from UNP as compared to urea for one of the three years. No such differences occurred with fescue. Nitrogen uptake was significantly higher from UNP as compared to urea for one year each for brome and fescue. Phosphorus uptake by brome was significantly higher from UNP as compared to urea for two years. Laboratory incubation studies showed significantly lower NH3 volatilization from UNP than from urea after seven days, but no significant differences after 14 days. The delay in NH3 volatilization was due to the diffusion and subsequent hydrolysis of urea immediately below the soil zone initially influenced by the UNP. The reduction in NH3 volatilization at the early time could partially be attributed to an inhibition of urea hydrolysis and significantly lower soil pH values for UNP as compared to urea in the upper 30 mm of soil cores. The general conclusion from the field and laboratory work was that UNP is a suitable N source for cool‐season grasses, with the primary potential benefit being delayed NH3 volatilization as compared to urea.  相似文献   

7.
Denitrification in subsoil(to a depth of 12 m) is an important mechanism to reduce nitrate(NO_3~-) leaching into groundwater.However, regulating mechanisms of subsoil denitrification, especially those in the deep subsoil beneath the crop root zone, have not been well documented. In this study, soil columns of 0–12 m depth were collected from intensively farmed fields in the North China Plain. The fields had received long-term nitrogen(N) fertilizer inputs at 0(N0), 200(N200) and 600(N600) kg N ha~(-1) year~(-1). Main soil properties related to denitrification, i.e., soil water content, NO_3~-, dissolved organic carbon(DOC), soil organic carbon(SOC),pH, denitrifying enzyme activity(DEA), and anaerobic denitrification rate(ADR), were determined. Statistical comparisons among the treatments were performed. The results showed that NO_3~- was more heavily accumulated in the entire soil profile of the N600 treatment, compared to the N0 and N200 treatments. The SOC, DOC, and ADR decreased with increasing soil depth in all treatments,whereas considerable DEA was observed throughout the subsoil. The long-term fertilizer rates affected ADR only in the upper 4 m soil layers. The ADRs in the N200 and N600 treatments were significantly correlated with DOC. Multiple regression analysis indicated that DOC rather than DEA was the key factor regulating denitrification beneath the root zone. Additional research is required to determine if carbon addition into subsoil can be a promising approach to enhance NO_3~- denitrification in the subsoil and consequently to mitigate groundwater NO_3~- contamination in the intensive farmlands.  相似文献   

8.
Nitrate (NO3?) can contribute to surface water eutrophication and is deemed harmful to human health if present at high concentrations in the drinking water. In grazed grassland, most of the NO3?‐N leaching occurs from animal urine‐N returns. The objective of this study was to determine the effectiveness of a nitrification inhibitor, dicyandiamide (DCD), in decreasing NO3? leaching in three different soils from different regions of New Zealand under two different rainfall conditions (1260 mm and 2145 mm p.a.), and explore the relationships between NO3?‐N leaching loss and ammonia oxidizing bacteria (AOB) and ammonia oxidizing archaea (AOA). The DCD nitrification inhibitor was found to be highly effective in decreasing NO3?‐N leaching losses from all three soils under both rainfall conditions. Total NO3?‐N leaching losses from the urine patch areas were decreased from 67.7–457.0 kg NO3?‐N/ha to 29.7–257.4 kg NO3?‐N/ha by the DCD treatment, giving an average decrease of 59%. The total NO3?‐N leaching losses were not significantly affected by the two different rainfall treatments. The total NO3?‐N leaching loss was significantly related to the amoA gene copy numbers of the AOB DNA and to nitrification rate in the soil but not to that of the AOA. These results suggest that the DCD nitrification inhibitor is highly effective in decreasing NO3? leaching under these different soil and rainfall conditions and that the amount of NO3?‐N leached is mainly related to the growth of the AOB population in the nitrogen rich urine patch soils of grazed grassland.  相似文献   

9.
Abstract

Six greenhouse pot experiments were conducted in which yield and nutrient uptake responses of corn (Zea mays L.) or tall fescue (Festuca arundinacea Shreb) to N, P, K, lime, or temperature variables were measured. This paper describes yield—NO3‐N and total N concentration trends in these experiments. Crop concentrations of total N and NO3‐N during early growth increased consistently with amount of applied N. These concentrations usually decreased (by dilution and assimilation) with time of growth and yield response to other growth‐limiting nutrients and other factors. Concentrations of K also increased in corn with amount of applied K, and high K was associated with higher concentrations of NO3‐N during early growth in some experiments. This was usually true only for early crop growth periods during which rates of nutrient uptake exceeded growth rate. High K concentrations may have retarded growth and NO3‐N reduction and assimilation.  相似文献   

10.
Abstract

The influence of nitrogen (N) fertilization on grass forage yield and quality as well as soil properties may vary with type of N fertilizer and time of application. The effects of 23 annual applications (from 1974 to 1996) of ammonium nitrate (AN) and urea (112 kg N ha‐1) applied in early fall, late fall, early spring and late spring on chemical soil properties and composition of bromegrass hay were evaluated in a field experiment on a thin Black Chernozemic soil located near Crossfield, Alberta, Canada. The influence of N addition, fertilizer type and application time on the soil properties was most pronounced in the 0–5 cm layer and declined in deeper soil layers. Application of N increased extractable ammonium (NH4)‐N, zinc (Zn), and iron (Fe) in the 0–5 cm layer; and sodium (Na), aluminum (Al), and manganese (Mn) in the 0–10 cm layer. But, N addition reduced extractable phosphoras (P) in the 0–30 cm; potassium (K) in the 0–60 cm; and pH, calcium (Ca), and magnesium (Mg) in the 0–5 cm soil layers. There was little effect of N fertilization on nitrate (NO3)‐N in soil. Soil pH, and extractable Ca and Mg in the 0–5 cm layer and Zn in the surface 15‐cm soil depth were lower with AN compared to urea, whereas the opposite was true for Fe, Mn, and Cu in the 0–5 cm layer and Na and Al in the top 15‐cm soil depth. Most of the changes in chemical soil properties due to N fertilization were reflected in elemental concentration of bromegrass hay, except for the increase of P concentration in bromegrass with N fertilization. In bromegrass hay for example, N addition increased total N and Cu with both N fertilizers and Mn and Zn with AN, but it lowered K and Ca with both fertilizers. There was more N and less Na with AN than urea in bromegrass hay. The effect of application time on chemical soil properties and composition of bromegrass hay was much less pronounced than N addition and fertilizer type. In conclusion, both N fertilizers changed chemical soil properties and composition of bromegrass hay, but the effects of 23 annual applications on soil properties were confined to shallow soil layers only. The greater lowering of soil pH with AN than urea may have implications of increased liming costs with AN.  相似文献   

11.
Switchgrass (Panicum virgatum L.) is a perennial biofuel crop with a high production potential and suitable for growth on marginal land. This study investigates the long-term planting effect of switchgrass on the dynamics of soil moisture, pH, organic carbon (SOC), total nitrogen (TN), nitrate nitrogen (NO3-N) and ammonium nitrogen (NH4+-N) for soils to a depth of 90-cm in a sandy wasteland, Inner Mongolia, China. After crop harvesting in 2015, soil samples were collected from under switchgrass stands established in 2006, 2008, and 2009, native mixture, and a control that was virgin sand. Averaged across six layers, soil moisture and pH was significantly higher under the native mixture than switchgrass or virgin sand. However, SOC and TN were significantly higher under the 2006 switchgrass stand when compared with all other vegetation treatments and the control. The SOC and TN increased from 2.37 and 0.26 g kg?1, respectively, for 2009 switchgrass stand, and to 3.21 and 0.42 g kg?1, respectively, for 2006 switchgrass stand. Meanwhile, SOC and TN contents were 2.51 and 0.27 g kg?1, respectively, under the native mixture. The soil beneath switchgrass and native mixture showed the highest NO3-N and NH4+-N, respectively. The soil moisture increased with depth while SOC, TN, and NO3-N decreased. An obvious trend of increasing moisture, SOC, TN, and mineral N was observed with increasing switchgrass stand age. Thus, growing switchgrass on sandy soils can enhance SOC and TN, improve the availability of mineral N, and generate more appropriate pH conditions for this energy cropping system.  相似文献   

12.
Abstract

Irrigation of untilled orchard floors can lead to substantial leaching losses of nitrate‐nitrogen (NO3‐N). Soil NO3 that remains after cool weather in the fall is subject to leaching in the spring. Nitrate losses can be controlled through growing ground cover vegetation to cycle residual nitrogen (N) and/or limiting the amount of water applied. A study was initiated in lysimeters to compare sodded soil surfaces versus bare soil for controlling NO3 leaching losses. Cool season vegetation (orchardgrass, western wheatgrass, white clover) and warm season grasses (bahiagrass and buffalograss) were compared for then‐effect on grapefruit seedling growth. A field verification in pecan orchards was conducted where clean‐till versus a grass soil cover was used to compare the relative movement of NO3 through the profile. The presence of vigorously growing sods greatly reduced NO3 losses the first year in the lysimeter study. The second year a shade screen was placed over the lysimeters, resulting in greatly reduced cool season sod growth and substantially reduced warm season sod growth. The best grapefruit growth occurred on bare soil; vigorous sod growth greatly reduced grapefruit tree growth. In the second year of the experiment, tree growth on bare soil began to absorb substantial amounts of N. The presence of even reduced receding sod growth still adversely affected grapefruit tree growth. In commercial pecan orchards, NO3 distributions in a clean‐tilled orchard soil showed large quantities of NO3 entering the water table (the highest quantity at the lowest depth of the soil profile) while in the presence of a sod much less NO3 (highest profile NO3 near the soil surface) was being lost to the water table. However, the NO3 leaching patterns were of large leaching losses in clean tilled surfaces and small controlled leaching losses with sod surfaces.  相似文献   

13.
Fertilizers applied to turfgrass may pose a threat to surface and groundwater quality, and hence, a study was carried out to evaluate the fate of fertilizer applied to turfgrass of the Clearwater Bay Golf and Country Club in Hong Kong. Lysimeters with reconstituted soils collected from fairways and greens with Bermudagrass (Cynodon dactylon) growing in the surface were used to evaluate the leaching loss of nitrate (NO3 -), ammonium (NH4 +), and phosphate (PO4 3-) for 22 weeks under greenhouse condition. Both soils received a slow release fertilizer at an application rate of 25 (low) or 50 (high) kg N ha-1, and an application frequency of monthly and fortnightly for fairways and greens, respectively, simulating the fertilizer application strategy of the golf course. Both low and high fertilizer application rate supported the same amount of biomass production for each soil type. Breakthrough of NO3 - occurred only in greens after 11 weeks of leaching, but the total amount of NO3 - leached did not differ significantly for the two different fertilizer application rates. The continued addition of fertilizer to greens resulted in a final leachate NO3 - concentration exceeding 45 mg L-1, while fairways maintained a concentration below 5 mg L-1. Also PO4 3- concentration in leachate of greens exceeded the surface water standard of 0.3 mg L-1. The results of the lysimeter study showed that the current application rate on greens would create adverse environmental impacts on the surface water and groundwater due to leaching loss of PO4 3 - and NO3 -.  相似文献   

14.
Contour ridging can enhance the occurrence of deep seepage because more rainwater concentrates in the low areas along furrows when soil is saturated. Greater seepage could significantly increase nonpoint source pollution. To optimize the contour ridge system to effectively control nutrient losses under seepage condition, 23 treatments with three variables (row grade, field slope and ridge height) in five levels were arranged in a quadratic orthogonal rotation combination design. Results showed that field slope and interactions between the three factors did not significantly influence NO3‐N and PO4‐P losses. The dominant factor that controlled NO3‐N loss was ridge height, followed by row grade. The smallest NO3‐N loss was obtained at a ridge height of 8.72 cm and a row grade of 7.05°. The dominant factor that controlled PO4‐P loss was ridge height. The optimal ridge height for efficiently controlling PO4‐P loss was 9.79 cm. For simultaneously maximally controlling NO3‐N and PO4‐P losses, a ridge height of 9.26 cm, a row grade of 7.05° and a field slope of 5° were optimum. This study provides guidance for implementing the contour ridge system.  相似文献   

15.
The method of fertilizer nitrogen (N) application can affect N uptake in tall fescue and therefore its yield and quality. Subsurface-banding (knife) of fertilizer maximizes fescue N uptake in the poorly-drained clay-pan soils of southeastern Kansas. This study was conducted to determine if knifed N results in greater N uptake than the conventional top-dress application method in a deep, well-drained soil of east-central Kansas. The experiment, conducted in a Smolan silty clay loam soil, was a split-plot with fertilizer nitrogen rates 0, 140 and 280 kg N ha?1 applied as urea-ammonium nitrate (UAN, 28% N), knifed or top-dressed. Soil inorganic N [ammonium (NH4)- and nitrate (NO3–N)] and N in roots and plant tops were measured at various times during the growing season. At final harvest, most of the knifed N (99.7%) was accounted for in plant tissue (roots and tops) and soil, with more than half of the knifed N remaining as soil inorganic N. With the top-dressed method, 27% was unaccounted for and presumed lost in gaseous form. Knifing fertilizer N in fescue fields of east-central Kansas will maximize the availability of N, reduce potential N losses, and increase forage quality.  相似文献   

16.
ABSTRACT

Nitrogen (N) absorption is inhibited by root zone salinity, which could result in increased NO3 leaching. Conversely, N absorption is enhanced by moderate N deficiency. Because turfgrasses are grown under N-limiting conditions, it is important to understand the interactive effects of salinity and N deficiency on N uptake. This study examined the effect of N status (replete versus deficient) and salinity on N (15NO3 and 15 NH4) uptake and partitioning by tall fescue (Festuca arundinacea Schreb.). Two cultivars (‘Monarch’ and ‘Finelawn I’) were grown in nutrient solution culture. Treatments included N level (100% or 25% of maximum N demand) and salinity (0, 40, 80, and 120 meq L?1) in a factorial arrangement. Absorption of NO3 and NH4 was greater in low-N than in high-N cultures, but was reduced by salinity under both N treatments. Salinity reduced partitioning of absorbed N to leaves and increased retention in roots. These results suggest that turfgrass managers should consider irrigation water quality when developing their fertilizer program.  相似文献   

17.
The capability to determine nitrogen availability of composts is necessary to ensure that such materials will provide sufficient fertilization to the growing crop and cause minimal environmental degradation. A greenhouse study using tall fescue as a bioindicator was used to evaluate nitrogen availability of two biosolids composts, two mixed yard waste-poultry manure composts, and one commercially-processed poultry litter. Five inorganic nitrogen (as NH4NO3-N) treatments applied at 0, 22.5, 45, 67.7, and 90 mg N/kg soil were employed to establish an N calibration curve. Yield, fescue biomass total nitrogen (as total Kjeldahl N (TKN)), and soil TKN and KCl extractable NO3?-N and NH4+-N concentrations of the organically amended treatments were compared to the inorganically fertilized treatments to determine amendment N mineralization rates and N fertilizer equivalent values (NFEV). Nitrogen mineralization rates were greatest in the poultry litter (21%) and Panorama yard waste compost (5%) amended pots. The NFEV of these amendments were 49% and 10%, respectively. Wolf Creek biosolids compost and Huck's Hen Blend yard waste compost immobilized N (?5% and 0.18%, respectively), and had percent NFEV of ?0.66% and 0.19%, respectively. Rivanna biosolids compost immobilized N (?15%), but the NFEV was 30% due to the relatively high inorganic N content in the amendment. Nitrogen mineralization and NFEV were generally greater in amendments with greater total N concentrations and lower C:N values. The total N concentration and C:N values were less reliable variables in predicting N mineralization and percent NFEV when a significant portion of the total N was in the inorganic form. Nitrogen equivalency value and N mineralization for each amendment increased with time of sampling, indicating the potential for early season N insufficiency to plants fertilized with compost due to lack of synchrony between N mineralization and plant N needs.  相似文献   

18.
Abstract

Plant growth in saline soils is regulated by the availability of nitrogen (N). High soil nitrate (NO3)‐N can lead to poor water quality. Many workers think that NO3‐N as a source for N can contribute to better plant growth in saline soils. The purpose of this work was to determine the necessity of NO3‐N and the ratio of NO3/ammonium (NH4) in the N fertilizer which gives higher productivity of the biomass yield of corn. Corn (Zea mays L.) plants (Var. LG11) were grown under saline soil conditions (8.5 dS m‐1), soils taken from the Euphrates valley (ACSAO Research Station) at Deir‐Ez‐Zor, east of Syria, from the surface layer of soil (0–25 cm). Five levels of N were applied in two forms, ammonium sulfate [15(NH4)2SO4] with enrichment (1.5% a) as the NH4‐N form and calcium nitrate [Ca(NO3)2] as the NO3‐N form, besides fixed amounts of phosphorus (P) and potassium (K) for all N treatments. The corn plants were harvested at the flowering stage (56 days old), oven dried, weighed, and analyzed for total N and 15N recovery. The results indicated that the dry matter weight for treatments which received a combination of NH4‐N and NO3‐N gave higher dry matter yield than a single treatment of one source of N. But, NO3‐N was more effective in improving yield than NH4‐N. Nitrogen recoveries on the basis of added and absorbed N derived from fertilizer were significantly more affected by NO3‐N than NH4‐N.  相似文献   

19.
Crop and native plants can be characterized as high and low nutrient‐adapted based on their expected response to native and applied nutrients. Our objective was to compare the plasticity of biomass allocation and tissue nutrient concentrations to added sulfur (S) and nitrogen (N) across a continuum of high and low nutrient‐adapted grasses, represented by barley (Hordeum vulgare), smooth brome (Bromus inermis), bluebunch wheatgrass (Pseudoroegneria spicata), and Idaho fescue (Festuca idahoensis). In our greenhouse study, treatments included two S sources (pyrite and gypsum), at 150 and 300 kg S ha‐1, N at 50 kg ha‐1, and a check. Shoot biomass of barley, smooth brome, and bluebunch wheatgrass was enhanced by S plus N. Shoot biomass of barley and smooth brome was greater with pyrite than with gypsum. Root biomass of smooth brome and bluebunch wheatgrass was greater with pyrite than with gypsum. Plant S concentrations of barley and Idaho fescue were enhanced by added S. Plant S concentrations in barley and smooth brome were greater with gypsum than with pyrite. Except for barley, plant S pools (shoot biomass x shoot S concentration) were enhanced with S plus N compared with no added nutrients. Nitrogen pools of barley, smooth brome, and bluebunch wheatgrass were higher with pyrite than with gypsum. Soil sulfate (SO4) was greater when S or S plus N was added than without any added nutrients. For barley and smooth brome, soil sulfate tended to be lower with pyrite than with gypsum. For all soils, pH was lower with added S or added S plus N compared with unamended soils. While pyrite lowered soil pH, gypsum tended to increase soil pH. Overall, barley and smooth brome were highly plastic in responding to enhanced nutrient levels, bluebunch wheatgrass was relatively responsive, and Idaho fescue was least responsive.  相似文献   

20.
Many state highway departments in the USA must use native plants for revegetating roadsides. We conducted two field studies in West Virginia to assess native plant establishment under two different conditions. On newly‐constructed sites, native species were seeded alone or combined with non‐native species. On older roadsides, native species were seeded in disturbed existing vegetation. In the first study, we used four seed mixtures comprised of seeds of native and non‐native species, and two N‐P‐K fertilizer treatments at three newly‐constructed sites. Native, warm‐season grasses were slow to establish and only contributed 25 per cent cover in some plots after three years. Indiangrass (Sorghastrum nutans [L.] Nash), big bluestem (Andropogon gerardii Vitman), Brown‐Eyed Susan (Rudbeckia triloba L.), and wild senna (Cassia hebecarpa Fernald) were the only seeded native species found. Fertilizer at 150 kg ha−1 of 10‐20‐10 showed little influence on increasing plant cover. In the second study, we disturbed three different‐aged established stands of vegetation composed of tall fescue (Festuca arundinacea Screb.) and crownvetch (Coronilla varia L.) by mowing, herbicide, or tillage, and native plants were seeded with and without fertilizer. Native cover was <10 per cent in all plots during the first year, but greatly increased by the second year to as much as 45 per cent in tilled plots, indicating that disturbance was necessary for natives to become important contributors within 2 years. Only switchgrass (Panicum virgatum L.), little bluestem (Andropogon scoparius Vitman), partridge pea (Chamaecrista fasciculate Michx.), and Brown‐Eyed Susan were observed in plots. Fertilizer at 300 kg ha−1 of 10‐20‐10 did not increase native plant cover on these sites. Based on our results, introducing or increasing the cover of native species along roadsides requires (1) reducing competition from non‐native species, and (2) longer time periods for these slower‐establishing species to be observed. Copyright © 2008 John Wiley & Sons, Ltd.  相似文献   

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