氮肥用量和喷施锌钾肥对小麦籽粒锌含量及生物有效性的影响

[目的]小麦籽粒锌(Zn)含量普遍较低,在没有外源锌施用措施下难以满足以小麦为主食人群健康所需.探索提升小麦籽粒Zn含量,尤其是其加工产品面粉Zn含量和Zn生物有效性的农艺措施,具有实际重要的意义.[方法]于2013、2014年分别在陕西杨凌示范区和三原试验站进行小麦田间试验,两地均为潜在缺锌石灰性土壤,DTPA-Zn...     

Predicting Wheat Quality Characteristics and Functionality Using Near‐Infrared Spectroscopy

The accuracy of using near‐infrared spectroscopy (NIRS) for predicting 186 grain, milling, flour, dough, and breadmaking quality parameters of 100 hard red winter (HRW) and 98 hard red spring (HRS) wheat and flour samples was evaluated. NIRS shows the potential for predicting protein content, moisture content, and flour color b* values with accuracies suitable for process control (R² > 0.97). Many other parameters were predicted with accuracies suitable for rough screening including test weight, average single kernel diameter and moisture content, SDS sedimentation volume, color a* values, total gluten content, mixograph, farinograph, and alveograph parameters, loaf volume, specific loaf volume, baking water absorption and mix time, gliadin and glutenin content, flour particle size, and the percentage of dark hard and vitreous kernels. Similar results were seen when analyzing data from either HRW or HRS wheat, and when predicting quality using spectra from either grain or flour. However, many attributes were correlated to protein content and this relationship influenced classification accuracies. When the influence of protein content was removed from the analyses, the only factors that could be predicted by NIRS with R² > 0.70 were moisture content, test weight, flour color, free lipids, flour particle size, and the percentage of dark hard and vitreous kernels. Thus, NIRS can be used to predict many grain quality and functionality traits, but mainly because of the high correlations of these traits to protein content.     

Effects of Soil Type and Tillage on Protein and Starch Quality in Three Related Wheat Genotypes

An experiment was conducted using three related wheat (Triticum aestivum L.) genotypes grown on two different soil types (a grey vertosol and a red kandosol) using two different tillage practices (complete and zero) to test the effects of environmental influence on grain quality. Wheat grains from plants grown in each environment were milled into flour; protein and starch were analyzed. The soil type had the biggest impact on both protein and starch content, with the grains from the grey vertosol soil having higher total, insoluble, and soluble protein contents, and lower starch content and flour swelling values. When protein was analyzed using SDS‐PAGE, the major difference observed between grains from the two soil types was an increased intensity of polypeptide bands corresponding to β‐amylase in grains from plants grown on grey vertosol soils.     

施氮量对冬小麦蛋白质品质和面粉色泽的影响

为探明不同施氮量对小麦籽粒产量、蛋白质含量及蛋白质组分、面粉色泽的调控效应,明确蛋白质含量及蛋白质组分与面粉色泽间的关系,在河南郑州进行了两年2种筋力型小麦品种（郑麦366和矮抗58）不同施氮水平的定点试验。结果表明,小麦籽粒产量、蛋白质产量和蛋白质含量均随施氮量的增加显著增加,两年结果表现一致。强筋小麦郑麦366产量和品质均提高的最佳施氮量为N 225 kg/hm2,而中筋小麦矮抗58为N 150~225 kg/hm2。蛋白质各组分随施氮量增加的变幅因品种不同存在差异。随施氮量增加两个小麦品种的面粉色泽亮度（L*值）降低,红度（a*值）增加。相关分析表明,面粉色泽亮度（L*值）与蛋白质和醇溶蛋白含量呈显著负相关（P0.05）,红度（a*值）与蛋白质及各蛋白质组分含量呈极显著正相关（P0.01）,黄度（b*值）仅与球蛋白含量呈极显著负相关（P0.01）。因此,施氮量显著影响小麦的籽粒产量和蛋白质含量,施氮量对面粉色泽的影响在不同品种和不同年度间存在差异。     

Morphological,Physical, and Chemical Properties of Grain and Flour from Chalky Rice Mutants

Morphological, physical, and chemical properties of grain and flour of 20 chalky rice mutants were compared with those of a translucent parental cultivar, Koshihikari. Compound starch granules were loosely packed and single starch granules were observed in chalky parts of mutant endosperm. Chalky mutant lines were classified into milky‐white and white‐core lines based on the degree of endosperm chalkiness. Physical and chemical characteristics also suggest a division of chalky mutant lines into two groups, consistent with the classification made based on morphological characteristics. Milky‐white mutant lines showed significantly lower grain weight, decreased starch content, and lower grain hardness than white‐core mutants and Koshihikari. Rice flour prepared from milky‐white mutants by dry milling showed less starch damage and finer mean particle size than white‐core mutants and the parent. These results indicated that a loosely packed structure in chalky endosperm was responsible for fragile grain and that it yielded fine flour with lower damaged starch contents. Decreased starch contents of chalky mutant lines correlated to lower RVA viscosities. Milky‐white mutant lines were expected to be useful to produce fine flour, which undergoes less starch damage during dry milling processing.     

In‐Depth Study of Durum Wheat Grain Tissue Distribution at Milling

The starchy endosperm proportion in durum wheat grain and its ability to be isolated from the peripheral tissues appear as main intrinsic characteristics potentially related to the milling value but still difficult to assess. In this study, several durum wheat samples displaying distinct grading characteristics were analyzed and processed through a pilot mill. The histological composition of grains and milling fractions was monitored by using identified biochemical markers of each wheat grain tissue. Contrasted milling yields of semolina and flour were observed between samples, despite displaying a similar starchy endosperm proportion determined by hand dissection. These yields were related both to differences in the starchy endosperm extraction and to the presence of the aleurone layer, particularly its cellular content. Furthermore, two distinct types of fractionation behavior of the aleurone layer were distinguished depending on the wheat grain sample. Extraction of the envelopes and embryonic axis into semolina and flours were found negligible in comparison with the other tissues.     

ATR-FTIR技术在小麦籽粒识别上的探索

为探索衰减全反射傅里叶变换红外光谱技术(ATR-FTIR)在小麦籽粒品种(系)识别和籽粒性状突变体鉴别上的应用,本研究采集了91个小麦品种(系)和576个小麦M5突变体及235个对照株系籽粒的ATR-FTIR光谱,其中71个小麦品种(系)用于建立小麦籽粒ATR-FTIR识别模型,20个小麦品种(系)用于外部验证;对建模...     

Front-face fluorescence spectroscopy as a rapid and nondestructive tool for differentiating various cereal products: a preliminary investigation

The potential of intrinsic fluorescence spectroscopy was investigated for differentiating between processed grains (flours, pasta, and semolinas) of different wheat cereal products. A total of 59 samples (e.g., 20 complete Kamut, semicomplete Kamut, and soft wheat flours, 28 pasta, and 11 semolinas manufactured from complete Kamut, semicomplete Kamut, and hard wheat flours) were analyzed by front-face fluorescence spectroscopy. Tryptophan fluorescence spectra were scanned between 305 and 400 nm on samples following excitation at 290 nm. The principal component analysis (PCA) performed on flour spectra clearly differentiated complete Kamut and semicomplete Kamut samples from those produced from complete and semicomplete soft wheat flours. The PCA performed on pasta spectra discriminated samples manufactured from complete Kamut and complete hard wheat flours from those made with semicomplete Kamut and semicomplete hard wheat flours. The best discrimination was obtained from tryptophan spectra recorded on semolinas since the four groups were well discriminated. Correct classification amounting to 61.9% was obtained for pasta spectra. A better classification was obtained for flour and semolina spectra since correct classification amounted to 86.7% and 87.9%, respectively. Front-face fluorescence spectroscopy has the potential to be a rapid, low-cost, and efficient method for the authentication of cereal products.     

Variation in Grain Mass,Grain Nitrogen,and Starch B-Granule Content Within Wheat Heads

Grain mass (mg) and grain nitrogen concentration (%) were determined in 3,278 individual grains from eight cultivars with reference to the position of the grain on the head (spikelet number and floret number). Selected grains were removed from certain heads at anthesis. Grain nitrogen content (mg) was determined as the product of grain nitrogen concentration and grain mass. Grain mass and starch B-granule content were determined in 3,030 grains from a further 12 cultivars with reference to grain position, and selected grains were removed from certain heads at anthesis. Grains from distal florets were always smaller and had lower B-granule contents, nitrogen contents, and nitrogen concentrations than those from the two proximal florets on each spikelet, which were not significantly different from each other. Grains in the basal two spikelets of the head were smaller with lower nitrogen contents and higher B-granule contents than those in most of the head. Their nitrogen concentration, however, did not differ from that in the rest of the head. All four traits declined in the grains in the top four to five spikelets of the head. The differences in grain mass, nitrogen, and B-granule content between florets within spikelets and between spikelets within the head varied with cultivar. Grains on treated heads were larger, higher in nitrogen and slightly lower in B-granule content than those on untreated heads. The effects of floret and spikelet on grain mass and nitrogen were not significantly changed by the treatments. Grains from florets 1 and 2, excluding those from the bottom three and top five spikelets, therefore represented a particularly uniform population for grain mass, protein nitrogen and starch granule composition. Genetic analyses based on samples of these grains will be more robust and repeatable than those based on unselected samples. Implications for improving seedling vigor, plant yield, and grain protein concentration in breeding programs are discussed.     

Lower 30 Minute Serum Insulin in Healthy Sprague‐Dawley Rats Consuming Chips from Specific Barley Flour Blends

Consumption of whole grains such as barley is known to inversely correlate with insulin resistance, type 2 diabetes, and other chronic diseases. However, data from randomized controlled trials in humans have not produced consistent results. Compositional differences between foods produced from different whole grains are likely to be a main reason for these discrepancies. The purpose of this study was to determine if varying barley flour composition achieved through milling influenced the postprandial glucose and insulin response. Specifically, this study aimed to test barley foods in the form of chips with very low hydration, thereby mimicking human snack foods. Fasted rats (N = 39) were randomized to one of four barley treatments, all produced from a single variety, Hordeum vulgare L. ‘CDC Fibar.’ The treatments used were straight‐grade flour (SGF), whole grain flour (WGF), bran flour with high β‐glucan (BF‐BG), bran flour with high insoluble dietary fiber, and wheat flour (WF) as the control. Blood was sampled over 120 min following treatment consumption to measure postprandial glucose and insulin concentrations. Our data showed that although there were no substantial glycemic or insulin effects following one‐time consumption of barley tortilla chips with specific compositions, those rats consuming WGF, SGF, or BF‐BG tended to have lower 30 min serum insulin concentrations compared with those rats consuming WF.     

Transient infrared spectroscopy for detection of toxigenic fungi in corn: potential for on-line evaluation

An urgent need for rapid sensors to detect contamination of food grains by toxigenic fungi such as Aspergillus flavus prompted research and development of Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) as a highly sensitive probe for fungi growing on the surfaces of individual corn kernels. However, the photoacoustic technique has limited potential for screening bulk corn because currently available photoacoustic detectors can accommodate only a single intact kernel at a time. Transient infrared spectroscopy (TIRS), on the other hand, is a promising new technique that can acquire analytically useful infrared spectra from a moving mass of solid materials. Therefore, the potential of TIRS for on-line, noncontact detection of A. flavus contamination in a moving bed of corn kernels was explored. Early test results based on visual inspection of TIRS spectral differences predict an 85% or 95% success rate in distinguishing healthy corn from grain infected with A. flavus. Four unique infrared spectral features which identified infected corn in FTIR-PAS were also found to be diagnostic in TIRS. Although the technology is still in its infancy, the preliminary results indicate that TIRS is a potentially effective screening method for bulk quantities of corn grain.     

Variation in Polyphenol Oxidase Activity and Quality Characteristics Among Hard White Wheat and Hard Red Winter Wheat Samples

Polyphenol oxidase (PPO) has been related to an undesirable brown discoloration of wheat-based end products. Consumer acceptance and product quality are generally decreased by the darkening phenomena. Two sets of wheat samples (Triticum aestivum L.) were investigated for variation in grain and flour PPO levels. Samples included 40 advanced experimental hard white winter wheat lines grown at two Kansas locations and 10 hard red winter wheat genotypes grown at three Nebraska locations. The variability in grain and flour PPO activities was influenced by growing location and population for the hard white wheat samples. There also was a significant influence of population by growing location interactions on PPO activity in both grain and flour. Genotype and growing location both contributed to variability in flour PPO activity among the hard red wheat samples. The variation in flour PPO activities among growing locations appeared larger than variation produced by genotypes tested for the hard red wheat samples. Quality parameters, such as wheat physical properties, flour protein and ash contents, grain color, and milling yield significantly correlated with grain and flour PPO activities. Among red wheat samples, flour PPO activity was related to 100 kernel weight, first reduction flour yield, and flour ash content. Grain PPO activity was related to variation in grain color observed among hard white samples. The relationship of quality characteristics with grain and flour PPO activities varied among white and red wheat samples.     

旱地高产小麦品种籽粒氮含量差异与氮磷钾吸收利用的关系

【目的】 调查高产小麦品种籽粒氮含量差异,探讨引起籽粒氮含量差异的主要农学和营养学因素,对于品种选育和优化养分管理,提高旱地小麦产量与营养品质有重要意义。 【方法】 于2013—2016年,以我国不同麦区的123个小麦品种为供试材料,在陕西渭北旱塬连续三年开展田间试验。设置施肥 (N 150 kg/hm2、P₂O₅ 100 kg/hm2) 和不施肥对照两个处理,收获后测产。在产量高于平均值的品种中,籽粒含氮量列前10名的定义为高氮品种,后10名的为低氮品种。分析了高产小麦品种植株和籽粒氮、磷、钾含量,干物质累积,产量构成及氮磷钾吸收利用的关系。 【结果】 小麦籽粒含氮量与产量呈极显著负相关,高产品种平均产量为6.9 t/hm2,籽粒产量每增加1000 kg/hm2,含氮量平均降低1.1 g/kg。高产品种间籽粒氮含量差异显著,高氮品种的籽粒含氮量平均分别为24.2 g/kg,低氮品种平均为19.4 g/kg,相差24.7%。两组品种的产量、生物量和产量构成因素差异均不显著,高氮品种的产量、生物量、收获指数、穗数和穗粒数对氮肥响应更敏感,施肥后分别显著增加70.0%、60.2%、9.8%、51.6%和14.3%,高氮品种营养器官含氮量较高,施肥后可显著增加150.0%;高、低氮品种籽粒的磷钾含量无显著差异,施肥后钾含量增加幅度均大于磷含量增加。高氮品种施肥后地上部氮、磷吸收量高于低氮品种,籽粒积累量增加幅度高于营养器官;籽粒钾吸收量无论施肥与否均显著低于低氮品种。施肥后,高氮品种地上部氮磷钾吸收量增幅高于低氮品种,营养器官的增幅高于籽粒。两类品种的氮磷收获指数无显著差异,但高氮品种的钾收获指数三年平均显著低于低氮品种。 【结论】 旱地土壤养分供应充足条件下,高产小麦高、低籽粒氮品种的产量、穗数、穗粒数和千粒重均无显著差异,但高籽粒氮品种对施肥响应更敏感。高产小麦品种间籽粒氮含量存在显著差异,高氮品种的籽粒含磷量高,含钾量低。施肥后,高氮品种的籽粒氮含量提高,磷、钾含量降低。高氮品种具有较高的地上部氮磷钾吸收量,但其向籽粒的转移能力并无优势。因此,在高产优质品种选育中,应进一步提升品种的氮磷钾收获指数,促进养分向籽粒分配,同时生产中需优化肥料投入,促进籽粒氮磷钾吸收与利用,实现产量和籽粒氮磷钾含量同步提高。      

Near-Infrared Reflectance Spectroscopy (NIRS) assessment of δ(18)O and nitrogen and ash contents for improved yield potential and drought adaptation in maize

The oxygen isotope composition (δ(18)O), accumulation of minerals (ash content), and nitrogen (N) content in plant tissues have been recently proposed as useful integrative physiological criteria associated with yield potential and drought resistance in maize. This study tested the ability of near-infrared reflectance spectroscopy (NIRS) to predict δ(18)O and ash and N contents in leaves and mature kernels of maize. The δ(18)O and ash and N contents were determined in leaf and kernel samples from a set of 15 inbreds and 18 hybrids grown in Mexico under full irrigation and two levels of drought stress. Calibration models between NIRS spectra and the measured variables were developed using modified partial least-squares regressions. Global models (which included inbred lines and hybrids) accurately predicted ash and N contents, whereas prediction of δ(18)O showed lower results. Moreover, in hybrids, NIRS clearly reflected genotypic differences in leaf and kernel ash and N contents within each water treatment. It was concluded that NIRS can be used as a rapid, cost-effective, and accurate method for predicting ash and N contents and as a method for screening δ(18)O in maize with promising applications in crop management and maize breeding programs for improved water and nitrogen use efficiency and grain quality.     

Einfluß von Stickstoff- und Cytokiningaben auf die Entwicklung des Fahnenblattes und der Karyopsen von Weizen

Influence of nitrogen and cytokinin applications on the development of flag leaves and grains of wheat In two experiments spring wheat cv. Solo was grown in hydroculture under greenhouse conditions. The influence of cytokinin applications during grain filling period (Cy: 0 – 100 μg BA/l nutrient solition) combined with different nitrogen supply (N: 0, 0.25, 0.50 and 4.0/1.3 mMol/l) was studied in regard to the development of flag leaves and grains. As to flag leaf, only N was capable to increase specific fresh and dry matter and delay the decrease during development. Similar changes in the chlorophyll content, photosynthesis and N-content of leaves and grains, resp., could be induced by either N or Cy. Grain growth and grain N-content was favoured by N, with the first effect becoming visible early and the second late in the grain filling period. At maturity Cy gave the same effects, but time course was slightly modified. At high doses of N (4.0/1.3 mMol/l) Cy-applications reduced chlorophyll content, photosynthesis and N-content of leaves and grains. This gave rise to the conclusion that cytokinins limit the corresponding physiological processes only if nitrogen nutrition is low.     

EFFECT OF SOIL AMENDMENT WITH ALFALFA POWDERS AND DISTILLERS GRAINS ON NUTRITION AND GROWTH OF CANOLA

Two pot experiments were carried out under controlled environment conditions in the growth chamber to assess the potential use of alfalfa powders and distiller grains as organic fertilizers. Two types of dehydrated alfalfa powders (one with canola meal protein extraction by-product and one without) and two types of distiller grains (dried distillers grain with distillation solubles added and wet distillers grain without solubles) from wheat-based ethanol production were evaluated. Four different nitrogen (N)-based amendment application rates (0, 100, 200 and 400 kg N ha^?1) were used along with urea applications made at the same N rates to a Brown Chernozem (Aridic Haploboroll) loamy textured soil collected from south-central Saskatchewan, Canada. Canola biomass yield, N, phosphorus (P), potassium (K), zinc (Zn), and cadmium (Cd) uptake were measured along with soil properties including pH, salinity, organic carbon, total nitrogen, phosphorus and extractable nutrients and cadmium before and after canola growth in each of the treatments. Application of alfalfa powder and distiller grain amendments resulted in significant canola biomass yield increases along with increased N, P, and K uptake compared to the unfertilized control. However, only a portion of the N added (～30% to 50%) in the organic amendments was rendered available over the five week duration of the experiments. Amendments that had higher N content and lower carbon (C):N ratios such as dried distillers grain with solubles resulted in greater canola N uptake. Reduced germination and emergence of canola seedlings was observed at high rates of addition of distillers grain (400 kg N ha^?1), the reason for which is unclear but may be due to a localized salt or toxicity effect of the amendment. The amendment with alfalfa powders and distiller grains resulted in small increases in residual soil nutrients. Effects on pH, salinity, organic carbon and extractable metals tended to be small and often not significant. Alfalfa powders and distillers grains appear to be quite effective in supplying nutrients, especially N, for plant growth over the short-term.     

Changes in anthocyanins in the grains of purple waxy hull-less barley during seed maturation and after harvest

Purple waxy hull-less barley cv. Daishimochi accumulates purple pigments in the stem, awn, lemma, palea, and pericarp during seed maturation. Four major anthocyanin constituents from the grains of cv. Daishimochi were isolated and identified as cyanidin 3-O-(3,6-di-O-malonyl-beta-D-glucopyranoside) (55%), cyanidin 3-O-(6-O-malonyl-beta-D-glucopyranoside) (21%), cyanidin 3-O-(3-O-malonyl-beta-D-glucopyranoside) (12%), and cyanidin 3-O-beta- d-glucopyranoside (4%) by mass spectrometry and one- and two-dimensional NMR spectroscopy. These anthocyanins were observed after 28 days after flowering (DAF); they were most abundant at 35 DAF when the dry weight of grains was maximum. This accumulation time was later than that of proanthocyanidins, which are the most abundant polyphenol constituents in barley grains. These anthocyanins, especially cyanidin 3-O-(3,6-di-O-malonyl-beta- d-glucopyranoside), decreased at 42 DAF and during drying preparation after harvest. Most anthocyanins are localized in the outer parts of grains and distributed into bran by the pearling process. Whole grain flour and bran of cv. Daishimochi are good sources of malonylated cyanidin derivatives.     

Impact of Bran Addition on Water Properties and Gluten Secondary Structure in Wheat Flour Doughs Studied by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy

The aim of this work was to elucidate the underlying physical mechanism(s) by which bran influences whole grain dough properties by monitoring the state of water and gluten secondary structure in wheat flour and bran doughs containing 35–50% moisture and 0–10% added bran. The system was studied with attenuated total reflectance (ATR) FTIR spectroscopy. Comparison of the OH stretch band of water in flour dough with that in H₂O‐D₂O mixtures having the same water content revealed the formation of two distinct water populations in flour dough corresponding to IR absorption frequencies at 3,600 and 3,200 cm^–1. The band intensity at 3,200 cm^–1, which is related to water bound to the dough matrix, decreased and shifted to lower frequencies with increasing moisture content of the dough. Addition of bran to the dough caused redistribution of water in the flour and bran dough system, as evidenced by shifts in OH stretch frequency in the 3,200 cm^–1 region to higher frequencies and a reduction in monomeric water (free water). This water redistribution affected the secondary structure of gluten in the dough, as evidenced by changes in the second‐derivative ATR‐FTIR difference spectra in the amide I region. Bran addition caused an increase in β‐sheet content and a decrease in β‐turn (β‐spiral) content. However, this bran‐induced transconformational change in gluten was more significant in the 2137 flour dough than in Overley flour dough. This study revealed that when bran is added to flour dough, water redistribution among dough components promotes partial dehydration of gluten and collapse of β‐spirals into β‐sheet structures. This transconformational change may be the physical basis for the poor quality of bread containing added bran.     

Quantitative analysis of soil nitrogen and carbon at a farm scale using visible and near infrared spectroscopy coupled with wavelength reduction

Reducing large spectral datasets to parsimonious representations of wavelengths is of value for efficient storage and easing analysis, in addition to the potential to use a simpler and cheaper spectrophotometer. This study evaluated the potential of calibrating visible and near infrared (vis‐NIR) spectra to total nitrogen (N), total carbon (C), organic C and inorganic C in soil on a 15‐ha farm, with the aim of comparing several wavelength reduction algorithms and rates in terms of model prediction accuracy. We explored the uninformative variables elimination (UVE), UVE coupled with successive projections algorithm (SPA) and two uniform‐interval wavelength reduction approaches (UWR‐I and UWR‐II) with successive wavelength reduction rates (WRRs) of 2, 5, 10, 20, 50, 100, 200, 500 and 1000. The standard normal variate (SNV)‐transformed absorbance spectra of soil samples recorded from 400 to 2499 nm at 1‐nm intervals were used. The calibration sets were subjected to a partial least squares regression (PLSR) with leave‐one‐out cross‐validation. Prediction results showed that UVE can reduce wavelength variables significantly while retaining good model prediction accuracy. The UVE‐SPA produced only three or four wavelengths, with which PLSR models achieved competitive prediction performance, compared with those based on all 2100 wavelengths, with coefficient of determination (R²) of 0.91, 0.89, 0.91 and 0.53 and residual prediction deviation (RPD) of 3.53, 2.95, 3.27 and 1.53 for soil total N, total C, organic C and inorganic C, respectively. The UWR tests showed that PLSR models responded insensitively to various WRRs from 2 to 100. The models calibrated for the 100‐nm interval spectra (21 remaining wavelengths) performed almost as well as those for the 1‐nm interval spectra. Although these findings might be valid only at the farm scale, it is recommended that the proposed wavelength reduction algorithms for more soil types and soils originated from larger areas should be examined.     

Tocopherol and Tocotrienol Content in Commercial Wheat Mill Streams

The content of tocopherols and tocotrienols, collectively known as vitamin E (tocols), was determined in fractions of roller‐milled wheat grains. The results showed that vitamin E components are present in all major flour fractions of wheat, but that the vitamin E content and composition differed significantly between fractions. The total content of vitamin E, calculated as alpha‐tocopherol equivalents, changed from 16.1 mg α‐TE/g in wheat grain to 12.2 mg α‐TE/g in roller‐milled wheat flour. The germ fraction had the highest content of tocopherols, and the content of α‐tocopherol (195.2 μg/g) was 16 times higher (on average) than in any other fraction. The content of tocotrienols was distributed more uniform in the wheat grain with the highest content in the bran fractions, and the content of β‐tocotrienol was higher than the content of α‐tocopherol in all milling fractions except the wheat germ. The content of β‐tocotrienol was 24.1 μg/g in wheat grain, 25.3–31.0 μg/g in the bran fractions, and 14.3–21.9 μg/g in the fractions of endosperm. Overall, germ and fine bran fractions represent good sources of vitamin E and might be used in breadmaking.