免疫塞内卡病毒灭活疫苗后豚鼠、家兔与猪的血清中和抗体相关性研究

为研究免疫塞内卡病毒(SVA)灭活疫苗后豚鼠、家兔与猪血清中和抗体的相关性,分别用0.25、0.5、1.0、2.0 mL的SVA灭活疫苗接种豚鼠和家兔,同时以2.0 mL的SVA灭活疫苗接种猪,分别于免疫前以及免疫后第7天、第14天、第21天、第28天采血液,测定各动物血清中SVA中和抗体,利用Microsoft Excel软件对所得结果进行整理并分析豚鼠与猪血清SVA中和抗体、家兔与猪血清SVA中和抗体之间的线性关系,进一步采用IBM SPSS Statistics 19软件对线性关系的显著性进行分析。结果显示,豚鼠和家兔均可产生SVA中和抗体,且免疫剂量越大,免疫动物产生的SVA中和抗体滴度越高。猪免疫后产生了SVA中和抗体,监测期内随着时间的延长,SVA中和抗体水平逐渐升高。当家兔和豚鼠免疫剂量为1.0 mL时,二者产生的SVA中和抗体与猪SVA中和抗体呈正相关,相关性分别为0.990和0.998,相关性极显著。研究表明,采用1.0 mL的剂量免疫豚鼠或家兔,能间接反应SVA灭活疫苗在猪体的SVA中和抗体水平。     

一起黑豚球虫病的诊断和治疗

湖南省某黑豚养殖基地黑豚存栏1 500多只,2014年10月暴发疾病,调查发现发病率100%,死亡率13.33%,造成了严重经济损失。临床观察发现黑豚食欲减退、腹泻及糊肛,病理学检查发现肠弥漫性出血,采集病死黑豚结肠组织进行镜检和制作石蜡切片,均发现球虫卵囊,诊断为球虫病。为了挽回经济损失,采用地克珠利抗球虫药配合黄芪多糖和维生素C进行治疗,经3~5 d病情得到有效控制,治愈率97.74%。     

Effects of Sulphur Dioxide on Guinea Pigs and Swine

Guinea pigs and swine were exposed to sulphur dioxide concentrations varying from 5-40 P.P.M.

The average daily weight gains of young guinea pigs were impaired by gas concentrations of 10 P.P.M. and 18 P.P.M. for periods of 96 hours or more. A single experiment failed to indicate any synergism between sulphur dioxide and hydrogen sulphide. Studies on the effect of exposure to 5 P.P.M. for an extended period were inconclusive.

Young swine under seven days of age were exposed to sulphur dioxide concentrations of 5, 10, 20, and 40 P.P.M. for a single eight-hour period for each group. All concentrations caused the animals to display some evidence of irritation from the gas, ranging from eye irritation, nasal secretion, salivation and altered respirations at levels of 10 P.P.M. and higher to slight eye irritation and salivation at levels of 5 P.P.M. Haemorrhage and emphysema were present in the lungs of swine exposed to 40 P.P.M., and sacrificed at twenty-four hours and seven days post-exposure. At 158 days post-exposure, two of two swine exposed to 40 P.P.M., and one of two swine exposed to 20 P.P.M. showed a pulmonary fibrosis that was attributed to the gas.

Impaired weight gains of exposed animals raised to market weight (158 days) could not be attributed to the gas.

     

人附红细胞体感染FMMU白化豚鼠的研究

用附红细胞体分别感染FMMU白化豚鼠和普通花色豚鼠，同时测定两组豚鼠的红细胞免疫功能，探讨FMMU白化豚鼠的免疫学特性与病原体敏感性之间的关系。结果表明，FMMU白化豚鼠对人附红细胞体比普通花色豚鼠敏感。封闭群FMMU白化豚鼠有独特的免疫学特性，红细胞免疫功能低于普通花色豚鼠，对病原体敏感性高于普通花色豚鼠，更适于建立感染性疾病动物模型。