共查询到18条相似文献,搜索用时 70 毫秒
1.
为了优化人工养殖大鲵(Andrias davidianus Blanchard)皮中胶原蛋白的提取工艺, 以加酶量、液料比和酶解时间为试验因素, 胶原蛋白提取率为响应值, 采用Box-Behnken设计进行试验。通过响应面法考察了3个因素对胶原蛋白提取率的影响, 建立了胶原蛋白提取率的二次多项式回归模型, 并对提取工艺进行了优化。试验结果表明, 3个因素对鲵皮中胶原蛋白提取率的影响大小次序为: 液料比>酶解时间>加酶量; 交互项中“液料比-酶解时间”项对提取率影响显著, 其他交互项对提取率影响不显著。为满足实际需要, 对模型预测得到的大鲵皮胶原蛋白最优提取工艺稍做调整, 具体参数为: 加酶量16.5%、液料比15 mL/g、酶解时间29 h。此条件下, 大鲵皮胶原蛋白提取率可达到66.99%, 与模型理论预测值(67.83%)较为一致。
2.
3.
于单因素试验基础上,以牡蛎蛋白和多糖提取率(Y1和Y2)为响应值,利用响应面法对牡蛎中蛋白和多糖提取工艺进行同步优化.试验结果表明,液料比(X1)、提取时间(X2)、提取温度(X3)及pH值(X4)4个因素对Y1和Y2均有显著影响.由响应面三维及等高线叠加图得到牡蛎蛋白和多糖提取率均高的最佳提取工艺参数:液料比33∶1,提取时间2.6h,提取温度40℃,pH值4.2.在此条件下,验证试验得到牡蛎蛋白提取率为21.15%,牡蛎多糖提取率为12.07%,与数学模型预测值非常接近.可见,响应面同步优化法对牡蛎蛋白和多糖提取条件进行同步优化合理可行. 相似文献
4.
缢蛏多糖的提取及抗氧化作用研究 总被引:1,自引:0,他引:1
以新鲜缢蛏的碎肉为材料,加水配制成一定比例的匀浆,利用超声波提取法对缢蛏碎肉匀浆进行多糖的提取。利用蒽酮—硫酸法对多糖得率进行计算。将得到的多糖粗品通过DEAE离子交换层析及G-75凝胶层析进一步分离纯化。通过邻苯三酚自氧化体系和Fenton体系测定所得样品的抗氧化能力。结果表明:利用超声波提取多糖的提取率为19.93%。多糖粗品通过DEAE离子交换柱,得到2个峰,峰1对超氧阴离子的清除能率为43.89%,质量浓度为1 mg/ml时,对.OH清除率为57.80%。峰2对超氧阴离子的清除能率为57.80%,质量浓度为1 mg/ml时,对.OH清除率为77.83%。将DEAE峰2进行G-75凝胶层析得到2个峰,峰1对超氧阴离子的清除能率为83.08%,质量浓度为1 mg/ml时,对.OH清除率为94.76%。峰2对超氧阴离子的清除能率为74.41%,质量浓度为1 mg/ml时,对.OH清除率为52.41%。 相似文献
5.
在单因素提取工艺优化的基础上,选取液料比、浸润时间、提取时间和提取温度进行四因素三水平的Box-behnken实验设计,并结合响应面分析法进一步优化丹参中抗无乳链球菌(Streptococcus agalactiae,SA)活性成分提取工艺。优化所得最佳提取工艺为液料比37.8:1.0(ml:g)、浸润时间104 min、提取温度86.7℃、提取时间182 min。响应面模型预测在该提取工艺下抗SA活性成分提取得率极值为25.95%,经4次平行验证实验的实际提取得率为(25.87±0.03)%,为优化前的2.59倍,表明优化方法高效可靠。 相似文献
6.
为了探究提取长茎葡萄蕨藻多糖的最优工艺及抗氧化活性,对目前已有的多糖提取方法进行筛选,并采用单因素实验和响应面实验的方法,对料液比、提取温度、提取时间、木瓜蛋白酶添加量和提取次数这5个因素进行优化。结果显示,添加木瓜蛋白酶提取长茎葡萄蕨藻多糖的方法最高效便捷,且当料液比1∶40,提取温度50 ℃,提取时间3 h,提取次数2次,以及木瓜蛋白酶添加量为2.0% 时,长茎葡萄蕨藻多糖提取率相对较高,可达到41.24%±0.09%。进一步的实验结果显示,长茎葡萄蕨藻多糖对DPPH和ABTS自由基均具有良好的清除活性,其IC50值分别为2.32和0.67 mg/mL。研究表明,使用优化后的木瓜蛋白酶酶解法能有效提高长茎葡萄蕨藻多糖的提取率,且长茎葡萄蕨藻多糖具有良好的抗氧化活性。本研究可为长茎葡萄蕨藻多糖的开发利用提供理论基础和参考依据。 相似文献
7.
8.
9.
10.
11.
采用三因素三水平中心组合试验设计方案,研究光照度、盐度、pH及三者交互作用对大竹蛏幼贝滤水率的影响,并进行响应面分析,探寻大竹蛏幼贝最适生长条件组合,构造大竹蛏幼贝滤水率模型。试验期为30d,试验光照梯度为2000lx、1000lx、0,盐度梯度为30、25、20,pH梯度为9.00、8.00、7.00。结果显示,光照度、盐度及pH三者对大竹蛏幼贝滤水率有显著交互作用(P0.05)。通过Design-Expert 8.0软件对数据进行二次多元回归,拟合得到大竹蛏幼贝滤水率y对编码自变量A(光照度)、B(盐度)和C(pH)的二次多元回归方程:y=1.62+0.084A+0.04B-0.14C-0.38A2-0.65B2-0.68C2(r2=0.9821),软件模拟最适大竹蛏幼贝生长的条件组合为光照度1109.17lx,盐度25.12,pH7.89。 相似文献
12.
乳山寨滩涂缢蛏半人工采苗的研究 总被引:1,自引:0,他引:1
为解决北方沿海缢蛏养殖的苗种问题,在乳山寨滩涂建立缢蛏半人工采苗场,对苗坪适时进行翻耕平整,加强培育管理,1993年4h采苗场共采蛏苗8770万粒,平均2215粒/,平均壳长2.5cm;1994年40h采苗场共采蛏苗5亿粒,平均1428粒/,平均壳长2cm。结果发现,附苗前翻松平整的苗坪附苗数量远高于未翻松平整的苗坪。山东沿海移养缢蛏性腺于9月中旬至10月上旬进入成熟期,10月上、中旬开始放散 相似文献
13.
响应面法优化四角蛤蜊酶解工艺条件 总被引:1,自引:0,他引:1
为提高四角蛤蜊的利用率,采用木瓜蛋白酶对其软体部位进行酶解。在单因素试验的基础上,研究温度、pH和酶加量对酶解过程的影响,通过三因素三水平的Box-Behnken响应面分析法优化其酶解工艺条件。试验结果表明,所建立的响应面模型极显著( P<0.0001),可以很好的反映各因素与水解度之间的关系,且由模型得出木瓜蛋白酶酶解四角蛤蜊的最佳工艺参数为温度48℃、p H 7.2、酶加量3000 U/g、料液比3∶100(g/m L )和时间3 h ,在此条件下水解度为37.05%,从而为四角蛤蜊的精深加工利用,尤其是天然海鲜调味料的开发提供理论依据。 相似文献
14.
15.
Reza Safari Reza Pourgholam Abbas Ali Motalebi Ahmad Ghoroghi 《Journal Of Aquatic Food Product Technology》2013,22(2):247-257
The objective of this study was to produce fish peptone (FP) from silver carp (Hypophthalmichthys molitrix) heads by Alcalase® hydrolysis in order to use for growing Vibrio anguillarum instead of the standard peptones applied in the commercial media. An optimization of the FP to be used as culture media was also carried out using the response surface method (RSM). According to the results, the degree of hydrolysis and protein percentage after 24 h was 37.68 mg/mL and 20.74%, respectively. The adjustment of the quadratic model with the experimental data was satisfactory. The analysis of variance showed a high coefficient of determination value (0.930). The results of fish protein hydrolysates also showed that fish by-products modified by enzymatic procedures can be used as low cost nitrogen sources for bacterial growth. 相似文献
16.
Minseok Yoon Jin-Soo Kim Min Young Um Hyejin Yang Jiyoung Kim Yun Tai Kim 《Journal Of Aquatic Food Product Technology》2017,26(7):801-810
Phlorotannins, marine polyphenols from the brown seaweeds, are a promising biologically active substance. This study aimed to optimize the extraction conditions for phlorotannin recovery from the brown seaweed Ecklonia cava using response surface methodology. Ethanol concentration (X1, %), extraction temperature (X2, °C), and extraction time (X3, h) were chosen as independent variables. The dependent variables were total phlorotannin content (TPhC; Y1) and yield of phlorotannins (Y2). Estimated optimal conditions were as follows: TPhC (Y1): X1 = 95%, X2 = 80°C, X3 = 22.8 h, and yield of phlorotannins (Y2): X1 = 88.3%, X2 = 80°C, X3 = 24 h. Predicted values of dependent variables under each optimal condition were TPhC (Y1) = 570.2 mg phloroglucinol equivalents/g (mg PGE/g, dry basis) and yield of phlorotannins (Y2) = 7.8% and were similar to the experimental values. In addition, content of dieckol, which is known as an indicator of phlorotannin products, was measured. Dieckol contents of the phlorotannin extracts with the highest (565.7 mg PGE/g) and lowest (315.4 mg PGE/g) TPhC were 49.1 and 34.4 mg/g (dry basis), respectively. 相似文献
17.
为比较文蛤红壳色选育系与江苏野生群体在不同条件下滤水率的差异并找出文蛤最佳滤水率条件,采用了试验生态学方法和响应面法对文蛤红壳色选育系幼贝进行滤水率的研究。试验结果显示,在一定范围内,文蛤幼贝滤水率随盐度、温度和藻类密度的增加而增大,超过一定范围,幼贝滤水率随盐度、温度和藻类密度的增加而减小;在同等条件下,文蛤红壳色选育系幼贝与野生群体滤水率无显著差异,但文蛤红壳色选育系生长速率显著高于野生群体;通过响应面法优化,文蛤红壳色选育系幼贝的最佳滤水率条件为:盐度21.82、温度27.40℃、藻类密度9.96×10^4个/mL,此条件下滤水率的预测值为1.62 mL/(个·min)。 相似文献
18.
Ling Wang Feng Tian Lu Xu Miaomiao Tao 《Journal Of Aquatic Food Product Technology》2017,26(2):224-233
Penaeus vannamei is the most widely aquacultured shrimp in the world. However, it is vulnerable to bacteria spoilage. Recently, the use of biological preservatives to defend against bacteria has attracted much attention. However, the practical effects of these preservatives remain unclear. In this work, response surface methodology with the Box-Behnken experimental design was adopted to investigate the effects of three biological preservatives—nisin, tea polyphenol (TP), and ε-polylysine (ε-PL)—on the bacterial growth in P. vannamei. The optimal preservative formula was obtained, which was nisin at 0.04 g/100 mL, ε-PL at 0.48 g/100 mL, and TP at 1.06 g/100 mL. Under the optimal conditions, shrimp had a total bacteria number as low as 1.81 × 103 CFU/g, which was not significantly different from the practical determinations. The findings indicated that this model was effective to analyze the antimicrobial effects of preservatives and to optimize the combination of biological preservatives. 相似文献