土壤水分胁迫对树木N2O排放速率的影响

采用封闭罩-气相色谱法观测研究了干旱胁迫对长白山阔叶红松林的几种优势树种-红松(Pinus koraiensis)、水曲柳(Fraxinus mandshurica)、胡桃楸(Juglans mandshurica)、椴树(Tilia amurensis)和蒙古栎(Quercus mongolica)叶片N2O排放。并同步测定5种树木叶片净光合速率、呼吸速率和气孔导度。结果表明：土壤水分胁迫明显降低树木叶片气孔导度、净光合速率和N2O排放速率，叶片气孔是树木N2O排放的主要通道。树木N2O排放以白天为主，在相同的水分条件下，不同的苗木有不同的N2O排放速率，同种苗木的N2O排放随干旱胁迫的加重而减少，在受到不同干旱胁迫时，针叶树红松N2O的排放速率降至正常水分条件下的34.43％和100．6％、阔叶树种N2O排放平均降至31．93％和86.35％。不同干旱胁迫的红松、水曲柳、胡逃楸、椴树和蒙古栎幼树叶片N2O排放速率为34．43、14．44、33．02、16.48和32．33ngN2O．g^-1DW．h^-1。图1表1参12。     

Effects of soil moisture and temperature on CH4 oxidation and N2O emission of forest soil

Soil samples were taken from depth of 0–12 cm in the virgin broad-leaved/Korean pine mixed forest in Changbai Mountain in April, 2000. 20 μL·L⁻¹ and 200 μL·L⁻¹ CH₄ and N₂O concentration were supplied for analysis. Laboratory study on CH₄ oxidation and N₂O emission in forest soil showed that fresh soil sample could oxidize atmospheric methane and product N₂O. Air-dried soil sample could not oxidize atmospheric methane, but could product N₂O. However, it could oxidize the supplied methane quickly when its concentration was higher than 20 μL·L⁻¹. The oxidation rate of methane was increased with its initial concentration. An addition of water to dry soil caused large pulse of N₂O emissions within 2 hours. There were curvilinear correlations between N₂O emission and temperature (r²=0.706, p<0.05), and between N₂O emission and water content (r²=0.2968, p <0.05). These suggested temperature and water content were important factors controlling N₂O emission. The correlation between CH₄ oxidization and temperature was also found while CH₄ was supplied 200 μL·L⁻¹ (r²=0.3573, p<0.05). Temperature was an important factor controlling CH₄ oxidation. However, when 20 μL·L⁻¹ CH₄ was supplied, there was no correlation among CH₄ oxidization, N₂O emission, temperature and water content. Foundation item: This paper was supported by Chinese Academy of Sciences. Biography: ZHANG Xiu-jun (1960-), female, Ph. Doctor, lecture in Laboratory of Ecological Process of Trace Substance in Terrestrial Ecosystem, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110015, P.R. China. Responsible editor: Song Funan     

Factors controlling N2O and CH4 fluxes in mixed broad-leaved/Korean pine forest of Changbai Mountain, China

Using the closed chamber technique, thein situ measurements of N₂O and CH₄ fluxes was conducted in a broad-leaved Korean pine mixed forest ecosystem in Changbai Mountain, China, from June 1994 to October 1995. The relationships between fluxes (N₂O and CH₄) and some major environmental factors (temperature, soil water content and soil available nitrogen) were studied. A significant positive correlation between N₂O emission and air/soil temperature was observed, but no significant correlation was found between N₂O emission and soil water content (SWC). This result showed that temperature was an important controlling factor of N₂O flux. There was a significant correlation between CH₄ uptake and SWC, but no significant correlation was found between CH₄ uptake and temperature. This suggested SWC was an important factor controlling CH₄ uptake. The very significant negative correlation between logarithmic N₂O flux and soil nitrate concentration, significant negative correlation between CH₄ flux and soil ammonium content were also found. This project is supported by Chinese Academy of Sciences Responsible editor: Chai Ruihai     

Effects of elevated CO2 on net photosynthetic rate of trees in Changbai Mountain

Net photosynthetic rates (NPRs) of four species seedlings,Pinus koraiensis, Pinus sylvestriformis, Fraxinus mandshurica andPhellodendron amurense, were measured at different CO₂ concentrations and time respectively in Changbai Mountain during the growing season in 1999. The seedlings were cultivated in open-top chambers (OTCs), located outdoors and exposed to natural sunlight. The experimental objects were divided into four groups by tree species. CO₂ concentrations in chambers were kept at 500 μL·L⁻¹ and 700 μL·L⁻¹ and contrast chamber and contrast field were set. The results showed that the effects of elevated CO₂ on NPR of the trees strongly depended on tree species and time. NPRs ofPinus koreainsis andPinus sylvestriformis seedlings increased with the rising of CO₂ concentration, while that ofPhellodendron amurense andFraxinus mandshurica increased at some time and decreased at another time. This project was supported by Chinese Academy of Sciences Responsible editor: Chai Ruihai     

Responses of the seedlings of five dominant tree species in Changbai Mountain to soil water stress

Soil water stress was studied on the potted seedlings of five dominant tree species (Pinus koraienes Sieb.et Zucc.,Fraxinus mandshurica Rupr., Juglans mandshurica Maxim, Tilia amurensis Rupr. and Quercus mongolica Fisch.ex Turcz) from the broadleaved/Korean pine forest in Changbai Mountain. Leaf growth, water transpiration and photosynthesis were compared for each species under three soil moisture conditions: 85%-100% (high water, CK), 65%-85% (Medium water, MW) and 45%-65% (low water, LW) of 37.4% water-holding capacity in field. The results showed that the characteristic of typical drought-resistance of the leaves is significantly developed. The net photosynthetic rate and water use efficiency of Fraxinus mandshurica were higher in MW than those in CK. But for the other four species, the net photosynthetic rate and water use efficiency in CK were lower than those in MW and LW. The transpiration rate responding to soil moistures varied from species to soecies.     

Response of seedlings of different tree species to elevated C02 in Changbai Mountain

Eco-physiological responses of seedlings of eight species,Pinus koraiensis, Picea koraiensis, Larix olgensis, Populus ussuriensis, Betula platyphylla, Tilia amurensis, Traxinus mandshurica andAcer mono from broadleaved/Korean pine forest, to elevated CO₂ were studied by using open-top chambers under natural sunlight in Changbai Mountain, China in two growing seasons (1998–1999). Two concentrations of CO₂ were designed: elevated CO₂ (700 μmol·mol⁻¹) and ambient CO₂ (400 μmol·mol⁻¹). The study results showed that the height growth of the tree seedlings grown at elevated CO₂ increased by about 10%–40% compared to those grown at ambient CO₂. And the water using efficiency of seedlings also followed the same tendency. However, the responses of seedlings in transpiration and chlorophyll content to elevated CO₂ varied with tree species. The broad-leaf tree species were more sensitive to the elevated CO₂ than conifer tree species. All seedlings showed a photosynthetic acclimation to long-term elevated CO₂. Foundation item: The project was supported by National Key Basic Development of China (G1999043400) and the grant KZCX 406-4, KZCX1 SW-01 of the Chinese Academy of Sciences Biography: WANG Miao (1964-), maie, associate professor in Institute of applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. China. Responsible editor: Song Funan     

Effects of elevated CO2 on net photosynthetic rate of trees in Changbai Mountain

lntroductionAtmosphericconcentrationofCo2isrisingdra-maticaIIyandadoubIingofthepresentconcentrationwouIdoccurinmidnextcentury,duetoburnoffossilfuelanddestroyofforest(Gates1983,Tauszetal.1996).EIevatedatmosphericCO2willchangeitsgradientdistributioninforestecoboundary,andwillproduceagreateffectontreesandthewholeforestecosystem.Atpresent,studiesoneffectsofelevatedCozonplantshavebeenapopuIartopic,especiallyoncropsrice(BateretaI.199o).HaveIkaetal.(1984)hasgainedmanyachievementsonsoybean.How-e…     

长白山典型阔叶红松林土壤二氧化碳排放通量

随着大气CO2浓度的升高,主要由其引起的温室效应与对生物新陈代谢的影响变得越来越显著。森林生态系统在全球碳循环中扮演着重要的角色。为了评估和理解森林土壤CO2通量及其随空气和土壤温度的季节和昼夜变化规律,我们在长白山北坡典型阔叶红松林内利用静态箱技术进行了原位观测。实验在整个生长季(6月初至9月末)昼夜进行,利用气相色谱进行气体分析。结果表明: 长白山阔叶红松林土壤是大气二氧化碳源,其CO2通量具有明显的季节和昼夜变化规律。通量的变化范围是(0.30-2.42)μmol穖-2穝-1,平均值为0.98μmol穖-2穝-1。土壤CO2排放的季节规律表明,土壤CO2通量的变化与气温和土壤温度的变化有关。CO2平均通量的最大值出现在7月((1.27±23%)μmol穖-2穝-1),最小值出现在9月((0.5±28%)μmol穖-2穝-1)。土壤CO2的昼夜波动与土壤温度变化有关,而在时间上滞后于温度的变化。森林下垫面土壤CO2通量与土壤温度显著相关,与6cm深度土层温度相关系数最大。基于气温和土壤温度计算的Q10值范围为2.09-3.40。图2表3参37。     

Soil CO2 evolution from Korean pine virgin forest at Changbai Mountain

The soil CO₂ evolution rate was measured in a virgin Korean pine forest. The results in June showed that the lowest value of evolution rate was 220 mg/(m²·h) and appeared at 6:00 a.m. The highest value was 460 mg/(m²·h) at 18:00. The rates of CO₂ evolution were related with soil temperature. On the basis of the constructed regression equation and the monthly average values of temperature, the magnitude of CO₂ evolution from Korean pine forest soil was 10.4 t/hm² during a growing season. This project was funded by the Opened Research Station of Changbai Mountain Forest Ecosystem, Chinese Academy of Sciences. Responsible editor: Zhu Hong     

Factors controlling N2O and CH4 fluxes in mixedbroad-leaved/Korean pine forest of Changbai Mountain, China

IntroductionTheincreasinggreenhousegasconcentrationshavereceivedmuchattention.TwoofthesegasesthatscientistsareveryconcernedaboutareN2oandCH`,becauseoftheirrapidincreaseandtheirim-portantchemistryintheatmosphere(Bouwman199o).ThemoIecuIegIobalwarmingpotentialofN2OandCH4areabout58and2o6timesthanthatofCO2.Inaddition,atmosphericconcentrationsofN2OandCH`areincreasingatahnualratesofo.25%ando.9%respectively(HoughtonetaI1992).Forestecosystemhasbeenknownasanimportantterres-trialecosystemattheasp…     

Age structure ofTilia broad-leaved Korean pine forest on northern slope of Changbai Mountain

Based on a vast of field investigation on stamps inTilia broad-leaved Korean pine forest on northern slope of Changbai Mountain, age structure of some major species were studied in this paper. The results showed that Korean pine population was composed of grouped patches with different ages. There were not strict intervals among the dominated generations, and the curve of age structure often had two or more peaks. The distribution of broad-leaved species in natural Korean pine forest was grouped or scattered, and age distribution was also uneven-aged. There existed close relation between quality of broad-leaved species and Korean pine. So, it shaped multi-storied and uneven-aged mixed forest. The model of age structure and growth demonstrated their passive correlation, but growth became slow when woods had reached old age. The study was funded by the Opened Research Station of Changbai Mountain Forest Ecosystems, the Chinese Academy of Sciences. (Reponsible Editor: Chai Rulhai)     

Effects of soil moisture and temperature on CH4 oxidation and N2O emission of forest soil

IntroductionMethane (CH4) and Nitrous oxide (NZO) are tWoimportant greenhouse gases that also play an important role in photochemical reactions in atmosphere.The global warming potential of CH4 and NZO areestimated tO be about 62 and 290 times that of carbon dioxide respeCtively. The concentration of thesegases have been increasing rapidly since the start ofthe industrial age, currently at rate of about 1% and0.25% per year respeCtively (Lelieveld et al. 1993),and 70%-90% of these gases …     

土壤温度和水分对长白山3种温带森林土壤呼吸的影响

为了研究土壤温度和土壤含水量对阔叶红松林(山地暗棕壤)、云冷杉暗针叶林(山地棕针叶林土壤)和岳桦林(生草森林土)的土壤呼吸的影响,于2001年9月在长白山进行了土壤实验。利用增加土壤样柱的含水量,将土壤含水量分为9%,、21%、30%、37%和43%5个等级,土壤样品分别在0、5、15、25和35的温度下保持24小时。阔叶红松林土壤在0~35范围内,土壤呼吸速率与温度呈正相关。在一定的含水量范围内(21%~37%),土壤呼吸随含水量的增加而升高,当含水量超出该范围,土壤呼吸速率则随含水量的变化而降低。土壤温度和水分对土壤呼吸作用存在明显的交互作用。不同森林类型土壤呼吸作用强弱存在显著差异,大小顺序为阔叶红松林>岳桦林>云冷杉暗针叶林.红松阔叶林土壤呼吸作用的最佳条件是土壤温度35,含水量37%;云冷杉暗针叶林下的山地棕色针叶土壤呼吸作用的最佳条件是25,21%;岳桦林土壤呼吸作用的最佳条件是35,含水量37%。但是,由于长白山阔叶红松林,云冷杉林和岳桦林处在不同的海拔带上,同期不同森林类型土壤温度各不相同,相差4~5,所以野外所测的同期的山地棕色针叶林土呼吸速率应低于暗棕色森林土呼吸速率,山地生草森林土呼吸速率应高于山地棕色针叶林土的呼吸速率。图2表1参25。     

Crop residue effect on crop performance,soil N<Subscript>2</Subscript>O and CO<Subscript>2</Subscript> emissions in alley cropping systems in subtropical China

Land management practices that simultaneously improve soil properties are crucial to high crop production and minimize detrimental impact on the environment. We examined the effects of crop residues on crop performance, the fluxes of soil N₂O and CO₂ under wheat-maize (WM) and/or faba bean-maize (FM) rotations in Amorpha fruticosa (A) and Vetiveria zizanioides (V) intercropping systems on a loamy clay soil, in subtropical China. Crop performance, soil N₂O and CO₂ as well as some potential factors such as soil water content, soil carbon, soil nitrogen, microbial biomass and N mineralization were recorded during 2006 maize crop cultivation. Soil N₂O and CO₂ fluxes are determined using a closed-based chamber. Maize yield was greater after faba bean than after wheat may be due to differences in supply of N from residues. The presence of hedgerow significantly improved maize grain yields. N₂O emissions from soils with maize were considerably greater after faba bean (345 g N₂O–N ha⁻¹) than after wheat (289 g N₂O–N ha⁻¹). However, the cumulated N₂O emissions did not differ significantly between WM and FM. The difference in N₂O emissions between WM and FM was mostly due to the amounts of crop residues. Hedgerow alley cropping tended to emit more N₂O than WM and FM, in particular A. fruticosa intercropping systems. Over the entire 118 days of measurement, the N₂O fluxes represented 534 g N₂O–N ha⁻¹ (AWM) and 512 g N₂O–N ha⁻¹ (AFM) under A. fruticosa species, 403 g N₂O–N ha⁻¹ (VWM) and 423 g N₂O–N ha⁻¹ (VFM) under Vetiver grass. We observed significantly higher CO₂ emission in AFM (5,335 kg CO₂–C ha⁻¹) from June to October, whereas no significant difference was observed among WM (3,480 kg CO₂–C ha⁻¹), FM (3,302 kg CO₂–C ha⁻¹), AWM (3,877 kg CO₂–C ha⁻¹), VWM (3,124 kg CO₂–C ha⁻¹) and VFM (3,309 kg CO₂–C ha⁻¹), indicating the importance of A. fruticosa along with faba bean residue on CO₂ fluxes. As a result, crop residues and land conversion from agricultural to agroforestry can, in turn, influence microbial biomass, N mineralization, soil C and N content, which can further alter the magnitude of crop growth, soil N₂O and CO₂ emissions in the present environmental conditions.     

Soil CO2 efflux in an Afromontane forest of Ethiopia as driven by seasonality and tree species

Variability of soil CO₂ efflux strongly depends on soil temperature, soil moisture and plant phenology. Separating the effects of these factors is critical to understand the belowground carbon dynamics of forest ecosystem. In Ethiopia with its unreliable seasonal rainfall, variability of soil CO₂ efflux may be particularly associated with seasonal variation. In this study, soil respiration was measured in nine plots under the canopies of three indigenous trees (Croton macrostachys, Podocarpus falcatus and Prunus africana) growing in an Afromontane forest of south-eastern Ethiopia. Our objectives were to investigate seasonal and diurnal variation in soil CO₂ flux rate as a function of soil temperature and soil moisture, and to investigate the impact of tree species composition on soil respiration. Results showed that soil respiration displayed strong seasonal patterns, being lower during dry periods and higher during wet periods. The dependence of soil respiration on soil moisture under the three tree species explained about 50% of the seasonal variability. The relation followed a Gaussian function, and indicated a decrease in soil respiration at soil volumetric water contents exceeding a threshold of about 30%. Under more moist conditions soil respiration is tentatively limited by low oxygen supply. On a diurnal basis temperature dependency was observed, but not during dry periods when plant and soil microbial activities were restrained by moisture deficiency. Tree species influenced soil respiration, and there was a significant interaction effect of tree species and soil moisture on soil CO₂ efflux variability. During wet (and cloudy) period, when shade tolerant late successional P. falcatus is having a physiological advantage, soil respiration under this tree species exceeded that under the other two species. In contrast, soil CO₂ efflux rates under light demanding pioneer C. macrostachys appeared to be least sensitive to dry (but sunny) conditions. This is probably related to the relatively higher carbon assimilation rates and associated root respiration. We conclude that besides the anticipated changes in precipitation pattern in Ethiopia any anthropogenic disturbance fostering the pioneer species may alter the future ecosystem carbon balance by its impact on soil respiration.     

土壤水分梯度对阔叶红松林结构的影响

2002年8月,在吉林省白河林业局红石林场(12755E,4230N),沿着一个山坡设置了一个长宽为112m8m、包含14个样方的样带。调查了群落结构、0-10cm和10-20cm的土壤含水量、枯落物现存量及其C、N、P含量,主要树种的叶片和枝条的C、N、P含量。沿着山坡的不同位置土壤含水量的不同导致阔叶红松林的群落结构发生变化。蒙古栎的比例随着土壤含水量的下降而逐渐升高,而其他主要阔叶树种则逐渐减少乃至消失。枯落物的水分变化趋势与土壤一致。在不同坡位枯落物的分解状况不同,干重差异显著。坡下枯落物含量较坡上的丰富,部分原因在于群落结构的变化。水分和养分含量的变化影响了枯落物的成分、降解及其养分的释放,进一步影响了林木的生长速度和林分结构并最终影响整个生态系统。图7表2参14。     

Testing the safety-net role of hedgerow tree roots by 15N placement at different soil depths

Trees which root below crops may have a beneficial role in simultaneous agroforestry systems by intercepting and recycling nutrients which leach below the crop rooting zone. They may also compete less strongly for nutrients than trees which root mainly within the same zone as crops. To test these hypotheses we placed highly enriched ¹⁵N-labelled ammonium sulphate at three depths in the soil between mixed hedgerows of the shallow-rooting Gliricidia sepium and the deep rooting Peltophorum dasyrrhachis. A year after the isotope application most of the residual ¹⁵N in the soil remained close to the injection points due to the joint application with a carbon source which promoted ¹⁵N immobilization. Temporal ¹⁵N uptake patterns (two-weekly leaf sub-sampling) as well as total ¹⁵N recovery measurements suggested that Peltophorum obtained more N from the subsoil than Gliricidia. Despite this Gliricidia appeared to compete weakly with the crop for N as it recovered little ¹⁵N from any depth but obtained an estimated 44–58% of its N from atmospheric N₂-fixation. Gliricidia took up an estimated 21 kg N ha^–1 and Peltophorum an estimated 42 kg N ha^–1 from beneath the main crop rooting zone. The results demonstrate that direct placement of ¹⁵N can be used to identify N sourcing by trees and crops in simultaneous agroforestry systems, although the heterogeneity of tree root distributions needs to be taken into account when designing experiments. This revised version was published online in June 2006 with corrections to the Cover Date.     

淹水胁迫对落羽杉等4个树种苗木生长的影响

采用人工控制模拟大田淹水,研究了淹水胁迫下乌桕、落羽杉、山核桃、水松4个树种在生长和形态特征上的响应。结果表明:(1)水松、落羽杉表现出较适应淹水的典型反应,在淹水茎部产生皮孔的膨大和不定根,试验结束时水松、落羽杉受淹的根系表现正常;乌桕、山核桃则表现出淹水胁迫下受到明显的伤害,叶片变小、叶片数量减少,叶片萎蔫、失绿、坏死、脱落;乌桕受淹根系部分腐烂,而山核桃受淹根系大部分腐烂,腐烂程度较乌桕严重。(2)涝处理不同程度地抑制了树种的苗高、地径、生物量、根干质量的积累,涝害指数、叶面积指数能够在一定程度上反映树种的耐涝性。(3)4个供试树种耐涝性可分为3个等级,抗涝型为水松、落羽杉,中等抗涝型为乌桕,不抗涝型为山核桃。     

Effects of winter selective tree harvest on soil microclimate and surface CO2 flux of a northern hardwood forest

Soil surface CO₂ flux (S_flux) is the second largest terrestrial ecosystem carbon flux, and may be affected by forest harvest. The effects of clearcutting on S_flux have been studied, but little is known about the effect of alternative harvesting methods such as selective tree harvest on S_flux. We measured S_flux before and after (i) the creation of forest canopy gaps (simulating group tree selection harvests) and (ii) mechanized winter harvest but no tree removal (simulating ground disturbance associated with logging). The experiment was carried out in a sugar maple dominated forest in the Flambeau River State Forest, Wisconsin. Pre-treatment measurements of soil moisture, temperature and S_flux were measured throughout the growing season of 2006. In January–February 2007, a harvester created the canopy gaps (200–380 m²). The mechanization treatment consisted of the harvester traveling through the plots for a similar amount of time as the gap plots, but no trees were cut. Soil moisture and temperature and S_flux were measured throughout the growing season for 1 year prior to harvest and for 2 years after harvest. Soil moisture and temperature were significantly greater in the gap than mechanized and control treatments. Instantaneous S_flux was positively correlated to soil moisture and soil temperature at 2 and 10 cm, but temperature at 10 cm was the single best predictor. Annual S_flux was not significantly different among treatments prior to winter 2007 harvest, and was not significantly different among treatments after harvest. Annual (+1 std. err.) S_flux averaged 967 + 72, 1011 + 72, and 1012 + 72 g C m⁻² year⁻¹ in the control, mechanized and gap treatments, respectively, for the 2-year post-treatment period. The results from this study suggest selective group tree harvest significantly increases soil moisture and temperature but does not significantly influence S_flux.     

双氧水处理对白蜡种子发芽的影响

在实验室进行了双氧水不同浓度和处理时间对白蜡种子发芽的影响研究,结果表明不同处理浓度（0％～4.0％）白蜡种子发芽率为64％～79％,有随浓度升高而增加的趋势,其中双氧水浓度为3.5％的效果最好,极显著高于其他处理（p＜0.01）,其他处理之间差异不显著（p＞0.05）;在双氧水处理浓度3.0％时,浸种12 h的发芽率为71％,极显著高于浸种24h时的42％发芽率（p＜0.01）.