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1.
Plant-growth promoting rhizobacteria (PGPR) play an important role in plant health and soil fertility. The experiment was conducted as factorial experiment with two factors of Azospirillum and Azotobacter. The bacterial strains were Azospirillum lipoferum s-21, A. brasilense DSM 1690, A. lipoferum DSM 1691, Azotobacter chroococcum s-5, and A. chroococcum DSM 2286. The results indicated that growth promotion by PGPR appears from early stages of growth, 45 days after inoculation (DAI). Beneficial effects of bacterial inoculation on ear growth were observed after 75 DAI. Inoculation with PGPR increased dry weights of leaf, stem, and grain and hence total biomass sampled at 90, 105, and 120 (harvest time) DAI. The greatest grain weight was produced by Azospirillum s-21 inoculation. Dual inoculation with Azotobacter s-5 + Azospirillum s-21 significantly increased total dry weight up to 115%. Results of this study showed that leaf area index and crop growth index were significantly affected by bacterial treatments.  相似文献   

2.
Abstract

To investigate the effect of biofertilizers on the growth and yield of Eucalyptus grandis seedlings, greenhouse experiments were performed applying fertilizers based on agricultural byproducts, inoculated with nitrogen-fixing bacteria of the genera Azotobacter spp and Azospirillum spp. For the biofertilizers formulation, a nitrogen-fixing bacteria consortium was inoculated, and the experimental design was a 2?×?2 × 2 factorial arrangement, the factors were nitrogen source (NS: chicken manure), source of carbon (CS: eucalyptus leaf litter) and source of micronutrients (RS: rhizospheric soil) with two dose levels, inoculated with a consortium of Azotobacter spp and Azospirillum spp. The optimal time production of the best biofertilizers was 30?days, with the highest density of Azospirillum (9.23 × 106 CFU·g?1) and Azotobacter (19.3 × 106 CFU·g?1), and total nitrogen contents in the range of 2.15-5.64%, released into the biofertilizers with chicken manure and bioaugmented with the bacterium consortium. The treatment with the highest dose of biofertilizer, 500?g, showed the most significant effect on seedling development, increasing growth, stimulating rooting and the highest increase in leaf number. The results show that biofertilizers contributed to Eucalyptus grandis crop yield, and biofertilizers are proposed as an alternative for implementing sustainable soil management in the forest sector.  相似文献   

3.
为高效提取黄秋葵中黄酮类化合物,本试验以黄秋葵中的4种主要黄酮类化合物和黄秋葵总黄酮含量为响应值,通过单因素试验和Box-Behnken响应面试验进行提取工艺优化,并采用牛津杯法考察黄秋葵总黄酮对6种常见致病菌的抑菌活性.结果表明,在提取温度37℃、提取时间20 min、乙醇浓度70%、料液比1:25 g·mL-1的条...  相似文献   

4.
Genetic Resources and Crop Evolution - Okra [Abelmoschus esculentus (L.) Monech.] is an annual herb of the family Malvaceae and has tremendous nutritional and industrial potential. However, the...  相似文献   

5.
探讨施氮量与灌水上下限对黄秋葵养分吸收的交互作用,为黄秋葵的水肥管理提供理论及参数依据。采用土壤盆栽试验,设置田间持水量的45%~55%即45%~55% FC(W1)、35%~65% FC(W2)、25%~75% FC(W3) 3种灌水上下限及0 kg/hm2(N0)、110 kg/hm2(N1)、330 kg/hm2(N2) 3个氮水平,观测不同施氮量和灌水上下限对黄秋葵生长发育、生理响应及养分吸收的影响。结果表明,相同施氮水平下,黄秋葵不同器官生物量、果实产量、灌溉水分生产率及氮磷钾养分积累量以W1最高。相同灌水上下限,黄秋葵不同器官生物量、果实产量、谷氨酰胺合成酶活性、灌溉水分生产率及氮肥偏生产力以N1最高,各处理之间差异显著。水、氮之间表现显著的互作效应,N1W1处理的黄秋葵总生物量、果实产量、灌溉水分生产率和氮肥偏生产力最高,分别为446.4 g、201.3 g、6.9 g/kg和108.7 kg/kg,显著高于最低处理N2W3(190.3 g、64.9 g、2.4 g/kg和11.7 kg/kg),分别提高了57.4%、67.8%、65.2%和89.2%。综合分析表明,高氮高水处理(N2W3)显著降低黄秋葵产量及水肥利用效率,黄秋葵产量、灌溉水分生产率及养分吸收综合体现的最优模式为110 kg/hm2的施氮量、45%~55%FC的灌水上下限(N1W1),此研究可为丰富黄秋葵高产栽培提供理论依据。  相似文献   

6.
Summary Factor, Principal component and canonical analyses were used to study the extent of genetic diversity among 30 accessions of West African okra. Genetic variability among the accessions proved to be large. Pigmentation of various parts of the accessions and fruit characteristics contributed significantly to the total variation observed. Factor analysis and principal component analysis produced similar results which were substantially different from those produced by canonical analysis. The first three canonical variables accounted for 100% of the total variance while the number of pods per plant, and pod weight primarily accounted for the first canonical variable. Whereas the second canonical variable was primarily loaded by number of seeds per pod and fruit colour, the third canonical variable was comprised of a weight of 100 seeds and number of epicalyx segments.The level of variability observed supports the opinion (Stevels, 1988, 1990) that this okra type constitutes a separate species.  相似文献   

7.
 Mixed inoculation of Vicia faba L. with four different Rhizobium/Azospirillum and Rhizobium/Azotobacter combinations led to changes in total content, concentration and/or distribution of the mineral macro- and micronutrients, K, P, Ca, Mg, Fe, B, Mn, Zn and Cu, when compared with plants inoculated with Rhizobium only. The effects varied to a great extent among the Azotobacter and Azospirillum strains selected for combined inoculation. Received: 6 June 1998  相似文献   

8.
This experiment evaluated the potentials of cocoa pod husk (CPH)-based compost on okra and soil chemical properties. Three CPH-based compost: CPH+ Neem leaf (CPH+ NL), CPH+ Poultry manure (CPH+ PM) and CPH+ PM+ NL were prepared. The treatments; 25, 50, 75, 100 kg N/ha of each compost and NPK mineral fertilizer at 40, 50, 60 kg N/ha and control, were applied to 5 kg soil each and arranged in a completely randomized design in three replicates. Two varieties of okra (NH47-4 and LD88) were grown for six weeks and residual effect evaluated. The Nitrogen, Phosphorus, and Potassium uptake of okra were determined. Pre- and post-cropping soil analyses were done. Data were analyzed using ANOVA and means separated by Duncan Multiple Range Test at α0.05. The results showed that the nutrient uptake of okra consistently increased with CPH-based compost compared to control in both main and residual cropping. Nitrogen uptake ranged from 53.6 (60 kg N/ha NPK) to 106.7 (50 kg N/ha CPH+ PM) and 16.10 (50 kg N/ha NPK) to 55.06 (25 kg N/ha CPH+ PM+ NL); Phosphorus uptake ranged from 6.9 (25 kg N/ha CPH+ NL) to 24.1 (60 kg N/ha NPK) and 3.70 (25 kg N/ha CPH+ NL) to 9.98 (50 kg N/ha CPH+ PM+ NL), while potassium uptake ranged from 166.4 (25 kg N/ha CPH+ NL) to 244.48 (25 kg N/ha CPH+ PM+ NL) and 64.06 (40 kg N/ha NPK) to 122.29 (75 kg N/ha CPH+ NL) mg/plant in main and residual cropping, respectively. Organic carbon, pH, nitrogen (N), phosphorus (P), potassium (K), magnesium (Mg) and sodium (Na) were also significantly increased by the CPH-based compost. It could, therefore, be concluded that CPH-based compost could be a good fertilizer for okra production.  相似文献   

9.
Abstract

Biofertilizers are an alternative to mineral fertilizers for increasing soil productivity and plant growth in sustainable agriculture. The objective of this study was to evaluate possible effects of three mineral fertilizers and four plant growth promoting rhizobacteria (PGPR) strains as biofertilizer on soil properties and seedling growth of barley (Hordeum vulgare) at three different soil bulk densities, and in three harvest periods. The application treatments included the control (without bacteria inoculation and mineral fertilizers), mineral fertilizers (N, NP and P) and plant growth promoting rhizobacteria species (Bacillus licheniformis RC04, Paenibacillus polymyxa RC05, Pseudomonas putida RC06, and Bacillus OSU-142) in sterilized soil. The PGPR, fungi, seedling growth, soil pH, organic matter content, available P and mineral nitrogen were determined in soil compacted artificially to three bulk density levels (1.1, 1.25 and 1.40 Mg m?3) at 15, 30, and 45 days of plant harvest. The results showed that all the inoculated bacteria contributed to the amount of mineral nitrogen. Seed inoculation significantly increased the count of bacteria and fungi. Data suggest that seed inoculation of barley with PGPR strains tested increased root weight by 9–12.2%, and shoot weight by 29.7–43.3% compared with control. The N, NP and P application, however, increased root weight up to 18.2, 25.0 and 7.4% and shoot weight by 31.6, 43.4 and 26.4%, respectively. Our data show that PGPR stimulate barley growth and could be used as an alternative to chemical fertilizer. Soil compaction hampers the beneficial plant growth promoting properties of PGPR and should be avoided.  相似文献   

10.
Strawberry (Fragaria ananassa cv. Aliso) plants were grown under three N‐rates in the nursery: 80, 320 and 640 kg N/ha, and then transplanted to the fruiting field. Plants from each nursery treatment were given 150, 300 and 450 kg N/ha. The fertilizer was given as ammonium sulfate in the nursery and as ammonium nitrate in the field, a part broadcasted before planting and the other part applied as top dressings. Biomass production, plantlets size distribution and N content were restricted by the lowest level of N given in the nursery. Plants grown at 320 kg N/ha in the nursery gave significantly earlier and higher total yields than those grown at the other rates. Fruit yields were not significantly influenced by the N‐application in the field. N fertilization in the early stages of strawberry plant development in the nursery is more important than later field application.

The influence of N fertilization on the growth and yield of strawberry plants has been studied by several workers. In some reports yields were increased when the rate of N applied was increased (1, 2, 3). In other studies no influence was found (4, 5, 6), or even a decrease in yield was reported as the rate of applied N was increased (7). While in some studies detrimental effects of N‐fertilizer on fruit yield were observed at 200–300 kg N/ha (8, 9), in other studies (6, 7) N applications up to 400 kg/ha did not affect yield. In Spain (3), adding up to 680 kg/ha caused an increase in fruit yield.

Even at relatively low applied‐N levels, up to 150 kg N/ha, strawberry response reports are not consistent. Some workers found that increasing N in this range caused an increase in fruit yield (8, 9, 3, 7, 10), while no effect was observed (4, 5, 6, 11) in several other studies. Most of the reported experiments with N‐fertilizer were carried out after plants were transplanted from the nursery to the field. To the best of our knowledge no attempt was made to evaluate the effect of fertilizer applied in the nursery.

The objective of this work was to evaluate the influence of different levels of N‐fertilizer, in both the nursery and the field, on strawberry plant development and composition, earliness, yield level and yield distribution.  相似文献   


11.
Plant growth promoting rhizobacteria (PGPR) enhance the plant growth directly by assisting in nutrient acquisition and modulating plant hormone levels, or indirectly by decreasing the inhibitory effects of various pathogens. The aim of this study was to select effective PGPR from a series of indigenous bacterial isolates by plant growth promotion and antifungal activity assays. This study confirmed that most of the isolates from maize rhizosphere were positive for PGPR properties by in vitro tests. Azotobacter and Bacillus isolates were better phosphate solubilizers and producers of lytic enzymes, hydrocyanic acid (HCN), and siderophores than Pseudomonas. Production of indole-3-acetic acid (IAA) and antifungal activity were the highest in Azotobacter, followed by Bacillus and Pseudomonas. The most effective Azotobacter isolates (Azt3, Azt6, Azt12) and Bacillus isolates (Bac10, Bac16) could be used as PGPR agents for improving maize productivity. Further selection of isolates will be necessary to determine their efficiency in different soils.  相似文献   

12.
The purposes of this study were evaluate the effects of biofertilizers inoculation on growth indices, yield and radiation use efficiency (RUE) in black cumin under Mashhad climatic conditions. This field experiment was carried out based on a randomized complete block design with three replications. This experiment was performed at Agricultural Research Station, Ferdowsi University of Mashhad, Iran, during two growing seasons 2007–2008 and 2008–2009. Treatments were (A) Azotobacter paspali, (B) Azospirillum brasilense, (C) Rhizophagus irregularis, A + C, B + C, A + B, A + B + C and control. In all treatments, except control, 100 g of seeds were inoculated with15 mg of each biofertilizer. Results indicated that leaf area index (LAI) and accumulative dry matter (DM) showed an increasing trend up to 1863°Cd and a short declining trend afterwards. The relation between accumulative DM and absorbed photosynthesis active radiation (PARa) was linear. Radiation use efficiency (RUE) fluctuated from 0.55 gMJ?1 (control) to 0.89 gMJ?1 (B+C). Inoculation with biofertilizers enhanced root development and hence availability of moisture and nutrients, particularly nitrogen and phosphorus. Since these ecological fertilizers produce many growth regulators for the plant which promote LAI and accumulative DM and therefore yield, PARa and RUE enhanced.  相似文献   

13.
Abstract

This investigation was conducted during two successive seasons (2014/2015) and (2015/2016) using 15?years old productive mango (Mangifera indica) trees cv. Zebda. The trees were grown at AlMalak Valley Farm, El-Sharkeya Governorate- Egypt (30–51° North; 32–53° East). Trees were planted 8?×?8 meter within and between rows in sandy soil under drip irrigation system using the Nile water. The objective of this study is to alleviate alternate bearing in cv. Zebda using mineral nutrients (nitrogen in the on year and boron in the off year). Treatments included three concentrations of nitrogen (1000, 1250, 1500?g/tree/year) and three concentrations of boron (0.0, 250, 500?mg L?1). Nitrogen was applied to the soil as ammonium sulfate and boron was applied as foliar spray of boric acid. The extra amount of nitrogen fertilizer (250 and 500?g N/tree) was applied at three installments in (May, June and July). Treatment was arranged in a factorial Completely Randomized Block Design with three replicates for each treatment. Results show that the on-year nitrogen fertilization significantly increased mango tree vegetative growth (number of shoot/branch, shoot length, shoot thickness, number of leaves/meter and leaf area) and yield. The average yield in the on year is 85.5?kg/tree at 1250?g N/tree but 67.4?Kilogram/tree at 1000?g N/tree (the control treatment). While in the off year boron foliar application resulted in a significant increase in flowering, initial fruit set, final fruit set and fruit yield. The average yield in the off year is 47?kg/tree at 250?mg L?1 boron but 9?kg/tree at 0.0?mg L?1 boron rate (the control treatment).The interaction treatment of 250?mg L?1 boron + 1500?g nitrogen/tree is the best treatment as it resulted in the highest values for all the tested parameters. The average yield of this treatment is 53.5?kg/tree. This treatment helps alleviate alternate bearing phenomenon by 41% and obtain the highest economic yield in the off-year, i.e. increased yield by 5.9 fold.  相似文献   

14.
The oil crop safflower may have a certain production potential under low‐input conditions (organic farming, developing countries), where the putatively low nutrient requirement is highly welcomed. However, current knowledge regarding the nutrient use efficiency of safflower as compared to similar oil crops is limited. It was thus the aim of this study to determine the potassium (K) use efficiency of safflower (Carthamus tinctorius L.) as compared to sunflower (Helianthus annuus L.). Safflower and sunflower were cultivated with increasing K supply in a mixture of equal volumes of sand, nutrient‐poor limed soil, and perlite in 5 L Mitscherlich pots. Both species responded strongly to increasing K supply with respect to growth and yield. Safflower out‐yielded sunflower at low K supply, while at high K level, the opposite was observed. Both species accumulated similar amounts of K in shoots at low K supply. Only at extremely low K supply, safflower took up more K than sunflower. However, achene yield of sunflower exceeded that of safflower at optimal and high K supply. Safflower utilized absorbed K more efficiently than sunflower to produce achene yield at suboptimal K supply in terms of both efficiency ratio and utilization index. The efficiency of a crop to use supplied or accumulated K for dry‐matter and achene production was interpreted in terms of Michaelis‐Menten kinetics, specifically addressing the shape of the yield response curve. Indeed, the efficiency of safflower to use K for growth and yield, analogue to a low Km in enzyme kinetics, was higher than in sunflower, while the K supply or K accumulation required to initiate yield formation in safflower was significantly lower. Similarly, safflower had a lower external K requirement for achene yield than sunflower at low and optimal K supplies. It can be concluded that safflower represents a low‐input crop and outperforms sunflower on soils low in available K. The data analysis also reveals that using just one efficiency indicator is usually not sufficient to adequately describe the K efficiency of the crop under consideration.  相似文献   

15.
Alternate partial root-zone drying (APRD) is a water-saving method but can regulate crop physiological responses. A pot experiment has been conducted to study the efficiency of partial and fixed root-zone drying on the growth and production of maize (Zea mays L.) in addition to the water use efficiency in soils with different gypsum content. The experimental treatments include three irrigation treatments, i.e. Conventional Irrigation (CI), Alternate Partial Root-zone Drying (APRD) and Fixed Partial Root-zone Drying (FPRD), and three soils with different gypsum content " (60.0 [G1], 153.7 [G2], and 314.2 [G3] g kg?1)". The vegetative growth, root dry mass and physiological indices (leaf relative water content, carotenoid concentration, proline) have been studied during three stages of maize plant growth (jointing, tasselling, and maturing). The Results showed that compared to CI, APRD and FPRD increased water use efficiency by 38.93 and 14.94% based on dry seed yield. In addition, compared to CI, APRD increased maize seed yield by 4.62–20.71%, while FPRD decreased yield by 19.24–5.28% for the gypsiferous soils G2 and G3, respectively. APRD has a slight effect on leaf water potential, leaf relative water content, carotenoid and proline activities from jointing to maturing stages at the three gypsiferous soils. Results suggest that APRD could make maize plants use water even more productively with better adaptation to water shortages in the gypsiferous soils.  相似文献   

16.
稻草还田对油菜生长、土壤温度及湿度的影响   总被引:13,自引:2,他引:11  
采用盆栽和田间试验相结合的方法,研究了不同稻草还田方式及还田量对油菜出苗及苗情和不同稻草覆盖量对油菜生长、养分吸收、产量及土壤水热状况的影响。盆栽试验结果表明,稻草覆盖和翻埋还田对油菜出苗均有抑制作用,出苗率及出苗速率随稻草还田量的增加均有降低趋势,但覆盖条件下油菜出苗所受抑制更加明显。稻草覆盖还田对油菜苗情影响不大,而稻草翻埋还田则对苗期油菜生长有明显的抑制作用。大田试验结果表明,稻草覆盖可以显著提高油菜苗期干物质积累及氮、磷、钾养分吸收的速率,但进入薹期之后油菜生长基本不受稻草覆盖的影响。稻草覆盖对油菜有显著的增产效果,平均增产幅度为18.1%,但不同覆盖量之间差异不显著。另外,稻草覆盖后,土壤温度日变化趋于缓和,土壤含水率明显提高,且随覆盖量增加稻草的保温保水效果更为明显。  相似文献   

17.
Our aim was to assess if Brassinolide (BR) could ameliorate stress caused by waterlogging on maize. Two BR levels (with and without), two maize varieties [Ikom White (IKW) and Obatanpa-98 (Oba-98)] and three growth stages [control (WLo), seedling stage (WL1), and tasseling stage (WL2)] were studied under waterlogging lasting 10 days. Maize growth and development were significantly (p?≤?.05) reduced by waterlogging stress under WL1 than WL2. Waterlogging stress at WL1 adversely affected (p?≤?.05) the protein and relative water contents. The nitrogen (N) content among the plant partitions (leaves, stems, and grains) were reduced (p?≤?.05) at both silking and harvest. The beneficial effect of BR was more pronounced in Oba-98 with higher protein contents, dry matter yield, N-uptake and harvest index than IKW. Oba-98 was also better yielding than IKW. Thus, in a waterlogged soil, treatment of maize plants with BR at WL1 could induce some tolerance.  相似文献   

18.
A two-year (2008–2009) field experiment was conducted to determine the effect of irrigation management and weed control on yield and yield components of safflower. The experiment was conducted as a split-split plot arranged in a randomized blocks design with three replications. There were two treatments (weedy and weed free) and four irrigation interval water managements (7 days for all stages, 15, 22 and 28 days after six-leaf stages). The results indicated that grain yield was 29% higher in weed-free plots than in weedy plots. Weed competition reduced yield components such as primary branches, petal weight and number of capitulum per plant. Increasing the irrigation interval to 15 days at the six-leaf stage had no significant effects on grain yield, but grain yield decreased by 18 and 29.8% with increases in the irrigation interval to 22 and 28 days, respectively. An increased irrigation interval >15 days had negative effects on yield components like number of capitulum per plant, petal weight and number of primary branches. The highest grain yield (3703 kg ha?1) was obtained in the weed-free plot, and there was no significant difference between 7- and 15-day irrigation intervals.  相似文献   

19.
土壤施硒对蚕豆出苗及生长指标的影响   总被引:2,自引:1,他引:1  
通过盆栽试验研究了崇礼蚕豆出苗及生长过程中不同指标对不同土壤硒浓度的响应。结果表明,低浓度硒(3.2 mg·kg-1)对蚕豆各出苗指标、叶绿素a含量、叶绿素a+b、叶绿素a/b及产量构成因素有一定的促进作用,但高浓度硒(12.4 mg·kg-1)对蚕豆出苗及生长指标有明显的抑制作用。通过各项指标与土壤硒浓度之间的相关分析及10%抑制率的临界指标EC10值表明,不同指标EC10各异,以出苗势或苗期干重作为蚕豆土壤临界值制定的依据更可靠,EC10分别为4.8和5.2 mg·kg-1。  相似文献   

20.
The Ethiopian region is characterised by a wide range of agro-climatic conditions, which accounted for the enormous resources of agro-biodiversity that exist in the country. The most important of these resources is the immense genetic diversity of the various crop plants in the country. Of these, one of the most on farm genetically diverse crops is sorghum. Since the advent of formal breeding in particular after green revolution, genetic diversity of most crops has been threatened worldwide. In order to assess on farm genetic erosion (GE), various research methodologies were employed. These were focused group interviews with 360 farmers, on farm monitoring and participation with 120 farmers, key informant interviews with 60 farmers and development agents, and semi-structured interviews with 250 farmers. Besides, diversity fairs were done with over 1200 farmers. Notwithstanding the complexity of assessing GE, it was assessed by various methods; namely, by temporal method (comparing 1960 and 2000 collections), area method, and semi-structured interview method at individual, community or wereda level and causes of varietal loss from other various perspectives. Farmers perceived GE as the reduced importance of the variety as indicated by lower proportion in the varietal portfolio. The five most important factors for varietal loss at individual farmers’ level were reduced benefit from the varieties, drought, Khat expansion, reduced land size and introduction of other food crops respectively. GE was not affected by wealth groups and ecological regions. Farmers do not make simple replacement as a strategic mechanism for genetic resources management. GE at regional level was quantified by temporal and spatial method. There was a complementation not rivalry between farmer varieties (FVs) and improved varieties (IVs). The whole process of GE is explained by three models, namely: Bioecogeographic enhanced genetic erosion model, Farmer induced genetic erosion model and Farmer-cum-bioecogeographic genetic erosion model. As aforementioned, sorghum genetic erosion behaviour is completely different from other food crops such as tetraploid wheat. The prediction in the late seventies that complete erosion of FVs by IVs by the end of the eighties, the principle of GE that competition between IVs and FVs, favours the former and results in the replacement of the latter is not valid in the context of sorghum in Ethiopia. Hence, maintenance of the on farm genetic diversity of sorghum is a reality but GE is rhetoric.  相似文献   

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