磷酸二氢钙和氯化钾对氯化铵处理黄泥土水溶性铵态氮和硝态氮的影响

氮磷钾是农业生产中大量施用并且经常共同施用的肥料,三者在土壤中的相互作用对养分的迁移转化、吸收和代谢有着深远影响.本文模拟生产中氮磷钾肥料同施,研究了田间持水量条件下磷酸二氢钙、氯化钾对氯化铵处理土壤水溶性铵态氮和硝态氮的影响.结果表明,铵态氮施入土壤后,随着培养时间的延长,土壤中水溶性铵态氮含量下降,硝态氮含量升高,两者之间存在着显著相关性.磷酸二氢钙延缓了铵态氮向其他形态氮的转变,使培养中期土壤水溶性铵态氮显著高于氯化铵处理土壤,并对培养中后期硝态氮的增加有抑制作用.氯化钾增加了培养前中期氯化铵处理土壤铵态含量,但显著抑制了氯化铵处理土壤培养后期硝态氮的含量.因此,农业生产中氯化铵和氯化钾共施,氯化铵和磷酸二氢钙共施,氯化铵、氯化钾和磷酸二氢钙共施,对提高氮肥利用率,降低硝态氮淋失损失均有重要作用.     

Impacts of silver nanoparticles on enzymatic activities, nitrifying bacteria, and nitrogen transformation in soil amended with ammonium and nitrate

Silver nanoparticles (AgNPs) are effective antimicrobial compounds that are used in a myriad of applications. Soil microorganisms play crucial roles in nitrogen cycling, but there is a lack of comprehensive understanding of the effects of AgNPs on enzymatic activity in the nitrogen cycle, nitrifying bacteria, and nitrogen transformation in soil. Herein, enzyme activities were determined following the addition of different forms of nitrogen, ammonium nitrogen ((NH₄)₂SO₄), nitrate nitrogen (KNO₃), and amide nitrogen (urea, CO(NH₂)₂) at 200 mg N kg^-1, into the soil amended with AgNPs at 0, 10, 50, and 100 mg kg^-1. After 7 d of incubation with 10 mg kg^-1 AgNPs, the activities of urease, nitrite reductase (NiR), nitrate reductase (NaR), and hydroxylamine reductase (HyR) were reduced by 12.5%, 25.0%, 12.2%, and 24.2%, respectively. Of particular note, more than 53.5%, 61.7%, and 34.7% of NaR, NiR, and HyR activities, respectively, were inhibited at 100 mg kg^-1AgNPs. The abundance (most probable number) of ammonia- and nitrite-oxidizing bacteria (AOB and NOB, respectively) was measured using real-time quantitative polymerase chain reaction (qPCR) and the Cochran method. The abundance of AOB and NOB decreased when AgNPs were present in the soil. The NH₄NO₃ amendment increased copy numbers of bacterial and archaeal amoA nitrification functional genes, by 38.3% and 12.4%, respectively, but AgNPs at 50 mg kg^-1 decreased these values by 70% and 56.4%, respectively. The results of ¹⁵N enrichment (atom% excess) of NH₄⁺ and NO₃^- experiments illustrated the influence of AgNPs on soil nitrogen transformation. According to the ¹⁵N atom% excess detected, the conversion of ¹⁵N-labeled NH₄⁺ to NO₃^- was significantly inhibited by the different levels of AgNPs in soil. The reduced gross nitrification rate further confirmed this finding. Overall, this study revealed considerable evidence that AgNPs inhibited nitrogen cycle enzyme activity, the number of nitrifying bacteria, the abundance of the amoA gene, and the gross nitrification rate. Silver nanoparticles inhibited nitrogen transformation, and the rate of nitrification was also negatively correlated with AgNP levels.     

Changes in biological properties and antioxidant capacity of an agricultural soil amended with sewage sludge

The effects of applying sewage sludge (SS) to agricultural soil (at low rate of 22.5, LRS, and at high rate of 45 t ha^?1 dry basis, HRS) were monitored over a 120-d experimental period. Total organic carbon (TOC), water-soluble organic carbon (WSOC), alkali-soluble phenols, basal respiration, specific enzyme activity, dehydrogenase activity (DH-ase), metabolic potential (MP) and FDA-hydrolytic activity (FDA) were strongly increased by both rates of SS applications. In the SS amended soil, about 70% of the organic C added with the material remained at the end of the experiment. Basal respiration increased with increasing SS doses. The specific enzyme activity and the MP indicate an increase in the enzyme activity in soil.

The addition of SS led to higher values than the control of all the tested parameters up to the end of the experimental period. The antioxidant capacity (trolox equivalent antioxidant capacity, TEAC) was influenced by SS addition only when applied at HRS. After 120 days only HRS value of TEAC (5.13 mM g^?1) was higher than control (4.09 mM g^?1). The pattern of TEAC did not enable any link to be established between antioxidant capacity and both alkali-soluble phenols and basal respiration in soil.     

Zinc effects on soil microflora and glucose metabolites in soil amended with 14C-glucose

Summary The effects of zinc added to a diluvial sandy clay loam soil on its microflora and the metabolic products of amended glucose in the soil were investigated, and its influences on both biological and chemical turnover are discussed.Changes in the soil microflora were followed by counting the microbes and measuring their contributions to soil respiration. The transformations of ¹⁴C-glucose products were traced in five divided fractions.Amended glucose was readily assimilated into microbial tissues and transformed to metabolites in the control soil. Within the initial 24 h of the incubation, most of the glucose was decomposed and about 40% of the substrate evolved as carbon dioxide. This primary metabolism was attributed to the bacterial population, and the subsequent secondary metabolism was associated with fungal growth rather thanbacteria. On the other hand, zinc (1000 g/g) added as chloride prolonged the primary metabolism of glucose and a large part of the incubation period for 96 h was occupied by this metabolism, which was mostly dependent on the fungal population. Viable bacterial number noticeably within the first 24 h of the incubation. During the course of the subsequent incubation, however, this number increased and the selection for zinc tolerance was suggested.     

Diffusion of ammonium and nitrate ions in flooded soil and nitrogen use efficiency of an irrigated rice system

Abstract

A laboratory study was used to simulate the pattern of diffusion of ammonium and nitrate ions in flooded soil. Ammonium, deep incorporated in a submerged irrigation system, diffused upward from the anaerobic to the aerobic layer where biochemical oxidation nitrified it to NO₂ and NO₃. These oxidized N species diffused downward from the aerobic layer to the anaerobic layer where most or at least partly, was lost as gaseous end products. Three crops of rice were grown in a glasshouse experiment to estimate N use efficiency under various combinations of irrigation and N management practices. Overall N use efficiency averaged 45%. Under continuous flooding, almost two thirds of the applied fertilizer N (647% use efficiency) was recovered by the rice crop. Under alternate flooding and drying, the response was very poor, with only about one fourth (26% use efficiency) of the applied fertilizer N being recovered by the crop. This demonstrated importance of the proper combination of irrigation and fertilizer management in paddy soils to maximize N utilization.     

Potassium,nitrogen, ammonium/nitrate ratio,and sodium chloride effects on wheat growth

Fertigation with KNO₃ as a means of reducing salinity hazards was tested with peanut (Arachis hypogaea) plants grown on dune sand, resulting in a reduction of plant growth and yield. The objective of this work was to study the interactions between N, K⁺ and NaCl as well as the effects of the NH₄ ⁺/NO₃ ^‐ ratio on vegetative and reproductive growth. Wheat (Triticum aestivum L.) plants were grown in polyethylene pots with fine calcareous dune sand with different proportions of NH₄ ⁺ and NO₃ ^‐, under saline (60 mM NaCl) and non‐saline conditions. Three replicates were harvested at the beginning of flowering, and one was grown to grain maturity. NaCl reduced shoot dry weight in all the treatments. Increasing the NH₄ ⁺ proportion in the total of 6 mM N in the nutrient solution, increased shoot dry weight, did not change nitrogen concentration in the dry mass but increased P percentage, either with or without 60 mM NaCl. The number of tillers produced in each treatment was correlated with dry matter yield. The effect of the NH₄ ⁺/NO₃ ^‐ ratio may be explained by alteration of the cation‐anion balance on the nutrient uptake by roots, which lowered pH of the nutrient solution with increasing NH₄ ⁺ concentration, by alteration of the cation‐anion balance on the nutrient uptake by roots, which lowered pH of the nutrient solution with increasing NH₄ ⁺ concentration.     

Nitrous oxide emissions from cattle-impacted pasture soil amended with nitrate and glucose

There is little information concerning N₂O fluxes in the pasture soil that has received large amounts of nutrients, such as urine and dung, for several years. The aims of this study were to (1) experimentally quantify the relationship between mineral N input and N₂O emissions from denitrification, (2) describe the time course of N₂O fluxes resulting in N inputs, and (3) find whether there exists an upper limit of the amount of nitrogen escaping the soil in the form of N₂O. The study site was a grassland used as a cattle overwintering area. It was amended with KNO₃ and glucose corresponding to 10–1,500 kg N and C per hectare, covering the range of nutrient inputs occurring in real field conditions. Using manual permanent chambers, N₂O fluxes from the soil were monitored for several days after the amendments. The peak N₂O emissions were up to 94 mg N₂O–N m⁻² h⁻¹, 5–8 h after amendment. No upper limit of N₂O emissions was detected as the emissions were directly related to the dose of nutrients in the whole range of amendments used, but the fluxes reflected the soil and environmental conditions, too. Thus, in three different experiments performed during the season, the total cumulative losses of N₂O–N ranged from 0.2 to 5.6% of the applied 500kg ha⁻¹. Splitting of high nutrient doses lowered the rate of N₂O fluxes after the first amendment, but the effect of splitting on the total amount of N₂O–N released from the soil was insignificant, as the initial lower values of emissions in the split variants were compensated for by a longer duration of gas fluxes. The results suggest that the cattle-impacted soil has the potential to metabolize large inputs of mineral nitrogen over short periods (∼days). Also, the emission factors for did not exceed values reported in literature.     

施用铵态氮对森林土壤硝态氮和铵态氮的影响

对取自武夷山的红壤、黄壤、黄壤性草甸土分别在对照(CK,N 0 mg/kg)、低氮(LN,N 50 mg/kg)、高氮(HN,N 100 mg/kg)3种氮(N)水平处理下开展培养实验,研究施加NH4+-N对森林土壤N转化的短期影响.结果表明,添加NH4+-N可显著(p＜0.05)降低土壤NO3--N含量4.5％～25.7％,但LN与HN处理差异不显著,NO3--N降低可能与NO3--N反硝化和异氧还原有关;然而,黄壤性草甸土NO3--N没有降低.与培养前比较,在第56天红壤NO3--N含量显著增加5倍左右;桐木关黄壤增加40％左右,而黄冈山25 km黄壤仅在CK处理下增加16％,但是黄壤性草甸土显著降低;结果显示LN与HN处理土壤NO3--N含量变化幅度小于CK.与CK相比,LN和HN处理红壤NH4+-N分别显著(p＜0.05)升高24.1％ ～ 96.5％和68.7％～114.1％,且随培养进行没有累积,可能与微生物固N有关;桐木关NH4+-N分别升高17.6％ ～ 39.6％和37.6％～95.8％ (p＜0.05),LN处理黄冈山25 km黄壤NH4+-N只有第7天升高17.8％ (p＜0.05),HN处理第7、14、28、42天显著升高17.5％～48.6％(p＜0.05).LN处理黄壤性草甸土的NH4+-N在前3周显著降低11.6％～28.5％ (p＜0.01); HN处理在第7天和14天分别降低10.8％(p＜0.01)和7.5％,但是在第28～56天显著增加17.6％～20.4％(p=0.002).随着培养进行,CK处理红壤NH4+-N逐渐降低,桐木关黄壤、黄冈山25 km黄壤和黄壤性草甸土升高;LN和HN处理黄壤和黄壤性草甸土NH4+-N逐渐升高.可见,不同海拔土壤类型对NH4+-N添加响应存在差异.     

Effects of glucose,cellulose, and humic acids on soil microbial eco‐physiology

Microbial eco‐physiology in soils is regulated by substrate quality of the organic matter. This regulation was studied for a forest and an agricultural soil by the combination of activity and biomass techniques. Soil respiration was stimulated by the substrate quality in the order, humic acid < cellulose < glucose over 20 days. Concurrently, substrate addition increased the respiratory quotient (RQ), defined as the ratio of mol CO₂ evolution per mol O₂ uptake. Anabolic processes were mainly induced by glucose addition. Soil preconditioned with glucose showed a decrease in the RQ value during glucose‐induced microbial growth in comparison to non‐amended control. The decrease in the RQ value induced by preconditioning with cellulose and humic acid was lower. Glucose, cellulose, and humic acid addition modified the microbial biomass as estimated by fumigation‐extraction (FE), substrate‐induced respiration (SIR), and ATP content. Since each biomass estimate refers to specific microbial components, shifts in microbial eco‐physiology and community structure induced by substrate quality were reflected by SIR : FE and SIR : ATP ratios. The active and glucose‐responsive biomass in the forest soil which was earlier suggested as being dominated by K‐strategists was increased in the order, humic acid < cellulose < glucose.     

Effect of rice plant growth on denitrification in rhizosphere soil

The effect of rice plant growth on the loss of basal nitrogen (N) through denitrification in the rhizosphere of subsurface soil was investigated by the ¹⁵N balance method. Labeled ¹⁵N was applied to the deep soil layer to distinguish between the N losses in the surface and subsurface soils. Denitrification in pots with whole plants (Control) was compared with that in pots with plants cut off at the base of the culm (Pcut) to evaluate the effect of plant growth on denitrification. The upward movement of the applied ¹⁵N from the deep soil was negligible. Thus, the amount of unrecovered ¹⁵N was equal to the amount of N lost through denitrification in the rhizosphere of the subsurface soil (20–150 mm soil depth). In the Control treatment, values of redox potential at 50 and 90 mm soil depths were negative throughout the experimental period. Therefore, it was assumed that the redox potential could not have been the limiting factor for the N loss through denitrification in this experiment. The α-naphthylamine-oxidizing activity of roots decreased drastically after the cutting treatment. The estimated amount of de nitrified ¹⁵N in the rhizosphere of the subsurface soil was significantly higher in the Pcut treatment than in the Control one at 30 and 40 d after transplanting (DAT), while it was comparable in the two treatments at 52 and 64 DAT. Since a greater amount of ¹⁵N loss was found to occur when there was no absorption of ¹⁵N by the plants, the absorption of ¹⁵N by plants may have contributed to the suppression of denitrification. The amount of immobilized ¹⁵N in the Control treatment was larger than that of the Pcut treatment throughout the experiment. N immobilization might have also contributed to the suppression of denitrification in the rhizosphere of the subsurface soil.     

秸秆还田对灌溉玉米田土壤反硝化及N2O排放的影响

运用乙炔抑制技术研究了不同施氮水平下秸秆还田对灌溉玉米田土壤反硝化反应和氧化亚氮(N2O)排放的影响。结果表明,土壤反硝化速率及N2O的排放受氮肥施用、秸秆处理方式及其交互作用的显著影响。与秸秆燃烧相比,不施氮或低施氮水平时,秸秆还田可刺激培养初期反硝化反应速率及N2O排放,增加培养期间N2O平均排放通量;高施氮水平时,秸秆还田可降低反硝化反应速率及反硝化过程中的N2O排放。秸秆还田可降低反硝化中N2O/N2的比例。     

Effect of carbon rate and type amended with ammonium or nitrate on nitrous oxide emissions in a strong ammonia oxidation soil

Journal of Soils and Sediments - The work aimed to (1) better understand how C rate and type affecting N2O emissions when combined application with different N forms in a strong ammonia oxidation...     

聚丙烯酸盐类土壤改良剂对燕麦土壤微生物量氮及酶活性的影响

以燕麦田土壤为研究对象,探讨了聚丙烯酸盐类土壤改良剂及其复配(聚丙烯酸钾、聚丙烯酰胺、腐植酸钾、聚丙烯酸钾+腐植酸钾、聚丙烯酰胺+腐植酸钾)对燕麦田土壤微生物量氮及土壤酶活性的影响。结果表明,不同土壤改良剂均能提高土壤有机质、碱解氮、速效磷和速效钾的含量,各指标分别比对照增加了8.24%～30.22%、7.60%～19.29%、5.15%～29.45%和27.86%～68.86%;土壤改良剂能促使燕麦全生育期内0～10、10～20和20～40 cm各土层的土壤微生物量氮含量显著提高,聚丙烯酸钾+腐植酸钾和聚丙烯酰胺+腐植酸钾复配处理较其各单施效果显著,随土壤深度的增加土壤微生物量氮逐层递减;与对照相比,土壤改良剂能显著提高燕麦全生育期各土层过氧化氢酶活性,在抽穗期活性最高,且以聚丙烯酸钾+腐植酸钾较高;但对于脲酶,聚丙烯酸钾+腐植酸钾、聚丙烯酰胺+腐植酸钾和腐植酸钾3个处理在苗期显著低于对照,在抽穗期和成熟期高于对照,两种酶活性均随土壤深度的增加逐渐降低。     

博德特氏菌HN36对土壤酶活性和呼吸强度的影响

研究了博德特氏菌HN36(Bordetella sp.)对受二氯喹啉酸污染土壤中的土壤酶活性和呼吸强度的影响。结果表明,与对照相比,加入HN36后均能提高土壤过氧化氢酶、蔗糖酶、多酚氧化酶、脲酶及碱性磷酸酶酶活性和呼吸强度,且随着接种量的增加而增强。当接种量为3×1010个.g-1干土时,效果最好。     

Effect of sewage sludge and ammonium nitrate on wheat yield and soil profile inorganic nitrogen accumulation 1

The beneficial effect of sewage sludge in crop production has been demonstrated, but there is concern regarding its contribution to nitrate (NO₃) leaching. The objectives of this study were to compare nitrogen (N) rates of sewage sludge and ammonium nitrate (NH₄NO₃) on soil profile (0–180 cm), inorganic N [ammonium nitrate (NH₄‐N) and nitrate nitrogen (NO₃‐N)] accumulation, yield, and N uptake in winter wheat (Triticum aestivum L.). One field experiment was established in 1993 that evaluated six N rates (0 to 540 kg·ha^‐1·yr^‐1) as dry anaerobically digested sewage sludge and ammonium nitrate. Lime application in 1993 (4.48 Mg ha^‐1) with 540 kg N ha^‐1·yr^‐1 was also evaluated. A laboratory incubation study was included to simulate N mineralization from sewage sludge applied at rates of 45, 180, and 540 kg N ha^‐1·yr^‐1. Treatments did not affect surface soil (0–30 cm) pH, organic carbon (C), and total N following the first (1994) and second (1995) harvest. Soil profile inorganic N accumulation increased when ≥270 kg N ha^‐1 was applied as ammonium nitrate. Less soil profile inorganic N accumulation was detected when lime was applied. In general, wheat yields and N uptake increased linearly with applied N as sewage sludge, while wheat yields and N uptake peaked at 270 kg N ha^‐1 when N was applied as ammonium nitrate. Lime did not affect yields or N uptake. Fertilizer N immobilization was expected to be high at this site where wheat was produced for the first time in over 10 years (previously in native bermudagrass). Estimated N use efficiency using sewage sludge in grain production was 20% (average of two harvests) compared to ammonium nitrate. Estimated plant N recovery was 17% for sewage sludge and 27% for ammonium nitrate.     

Influence of the water regime on denitrification and aerobic respiration in soil

Summary The influence of soil moisture on denitrification and aerobic respiration was studied in a mull rendzina soil. N₂O formation did not occur below –30 kPa matric water potential (_m), above 0.28 air-filled porosity (a) and below 0.55 fractional water saturation (_v/PV volumetric water content/total pore volume). Half maximum rates of N₂O production and O₂ consumption were obtained between _m = –1.2 and –12 kPa,a = 0.05 and 0.23, and _v/PV = 0.63 and 0.92. No oxygen consumption was measured at _v/PC 1.17. O₂ uptake and denitrification occurred simultaneously arounda = 0.10 (at _m = –10 kPa and _v/PV = 0.81) at mean rates of 3.5 µl O₂ and 0.3 µl N₂ h^–1g^–1 soil. Undisturbed, field-moist soil saturated with nitrate solution showed constant consumption and production rates, respectively, of 0.6 µl O and 0.22 µl N₂O h^–1g^–1 soil, whereas the rates of air-dried remoistened soil were at least 10 times these values. The highest rates obtained in remoistened soil amended with glucose and nitrate were 130 µl O₂ and 27 µl N₂O h^–1g^–1 soil.     

红壤丘陵区人工林恢复措施对土壤酶活性和微生物学性质的影响

我国红壤丘陵区水土流失较严重。本文研究人工林对土壤(0～20和20～40 cm)酶活性和微生物学性质的短期(13～14年)影响。土壤转化酶、脲酶、多酚氧化酶活性在次生天然林和湿地松樟树混交林中高于其他林分。次生天然林和湿地松樟树混交林土壤微生物生物量和土壤呼吸值较大、有机碳活性较强、土壤微生物基质利用效率较高。因此,短期影响下,湿地松樟树混交林较之针叶纯林和针叶混交林可改善土壤生化强度和微生物活性。     

土壤辐照灭菌对土壤中铵态氮和硝态氮行为的影响

拟通过土壤辐照灭菌的方法,研究土壤微生物对硝态氮和铵态氮在土壤中的相互转化、固持及损失的影响,为提高作物氮肥利用率提供理论依据。采用土壤纯培养的方法,通过外源添加~(15)N标记铵态氮肥[(~(15)NH_4)_2SO_4]和硝态氮肥(Na~(15)NO_3),结合γ辐照灭菌的方法,培养30 d后,测定分析了灭菌和未灭菌土壤中总的、来自于肥料的和来自于土壤的铵态氮和硝态氮含量,并定量评价了肥料氮在土壤中的残留、固持和损失情况。结果表明:灭菌显著抑制铵态氮向硝态氮的转化,激发土壤铵态氮的释放,对铵态氮在土壤中的残留、固持和损失没有显著影响;灭菌对土壤硝态氮转化为铵态氮的过程没有影响,降低了硝态氮在土壤中的残留和固持,增加了硝态氮的损失;与外源添加硝态氮相比,外源添加铵态氮促进了土壤自身无机氮的释放,外源添加的铵态氮在土壤中残留低、固持高、损失高。因此,总体来看,灭菌有利于土壤铵态氮的积累,却降低土壤硝态氮的积累。虽然外源铵态氮较外源硝态氮更能激发土壤无机态氮的释放,并更易被土壤固持,但是铵态氮肥较硝态氮肥在土壤中残留少、损失多。     

Losses of nitrogen by denitrification from a grassland soil fertilized with cattle slurry and calcium nitrate

Losses of N by denitrification from an imperfectly drained grassland soil were measured by the acetylene-inhibition technique over a 1-yr period, during which applications of up to 200 kg ha ^?1 of N as cattle slurry or calcium nitrate were made. The quantities of N lost from nitrate-treated soil were much greater than from slurry-treated areas, and ranged up to 21% of the N applied. The losses occurred predominantly over brief periods following fertilizer application in the spring. Ratios of N released as N₂ to that released as N₂O increased as denitrification rates increased. The highest ratio recorded, 24, may have been a conservative estimate because inhibition of N₂O reduction may not have been complete on all occasions. Increased respiration was observed in the soil profile as a result of adding C₂H₂. This effect should be taken into account in interpreting experiments using the C₂H₂-inhibition technique.