温室菜园土壤氮矿化和微生物群落对水分的响应

Soil drying and wetting impose significant influences on soil nitrogen (N) dynamics and microbial communities. However, effects of drying-wetting cycles, while common in vegetable soils, especially under greenhouse conditions, have not been well studied. In this study, two greenhouse vegetable soils, which were collected from Xinji (XJ) and Hangzhou (HZ), China, were maintained at 30% and 75% water-holding capacity (WHC), or five cycles of 75% WHC followed by a 7-day dry-down to 30% WHC (DW). Soil inorganic N content increased during incubation. Net N mineralization (Nmin), microbial activity, and microbial biomass were significantly higher in the DW treatment than in the 30% and 75% WHC treatments. The higher water content (75% WHC) treatment had higher Nmin, microbial activity, and microbial biomass than the lower water content treatment (30% WHC). Multivariate analyses of community-level physiological profile (CLPP) and phospholipid fatty acid (PLFA) data indicated that soil moisture regime had a significant effect on soil microbial community substrate utilization pattern and microbial community composition. The significant positive correlation between Nmin and microbial substrate utilization or PLFAs suggested that soil N mineralization had a close relationship with microbial community.     

Properties and metabolic diversity of microbial communities in soils treated with steam sterilization compared with methyl bromide and chloropicrin fumigations

The effects of steam sterilization (SS) on soil microbial properties including metabolic diversity of the microbial communities were examined in a greenhouse compared with those of two fumigants, methyl bromide (MB) and chloropicrin (CP). The numbers of fungi decreased in all the treatments. Nitrifiers, both ammonium-oxidizing bacteria and nitrite-oxidizing bacteria, were severely affected by the SS and CP treatments, resulting in their virtual disappearance. The decrease in the levels of microbial biomass C and N after the treatments suggested that the SS and CP treatments eradicated the microorganisms more effectively than the MB treatment and that the influence of the former persisted until the end of the experiment, 4 months after the treatments. Accumulation of NH₄-N was observed after the SS and CP treatments mainly due to the partial decomposition of the dead microorganisms and the marked decrease in the number of ammonium-oxidizing bacteria. The richness and average well color development (AWCD) values in the microbial communities after the SS treatment were evaluated by the carbon substrate utilization method using Biolog ECO MicroPlates. The values decreased markedly immediately after the treatment but showed a rapid recovery, while those after the CP treatment continued to decrease until the transplanting of tomato seedlings. The effect of the MB treatment on the soil microbial communities was less pronounced. The growth of the tomato plants was promoted by the SS and CP treatments due to the increase in the N supply at the initial stage of tomato growth.     

土壤微生物群落对高剂量的新鲜橄榄油厂废水的响应

An incubation experiment was designed in order to determine the further microbiological response to an addition (500 m³ ha^-1) of fresh olive mill wastewater (FOMWW) in a soil that has been frequently amended with uncontrolled doses of OMWW since 90s in an active disposal site (ADS soil). To achieve this aim, the phospholipid fatty acid (PLFA) profiles, microbial biomass C (C_mic), and dehydrogenase (DHA) and urease activities (URA) were monitored at the beginning (T₀), 3 h (T₁) and 97 d (T_f, i.e., the end) of incubation after FOMWW addition. After the FOMWW addition, an increase in the ratio of fungal to bacterial PLFAs was observed in ADS soil. Moreover, a relative increase of monounsaturated fatty acids (MUFAs) with respect to saturated fatty acids (SATFA) was found in the ADS soil. An increase of the Gram-positive to Gram-negative ratio was observed in this soil at the end of the incubation. While DHA and Cmic increased in the ADS soil after FOMWW addition, URA showed a decrease. Fungi and Gram-positive bacterial biomass experienced an increase after addition of a high dose of FOMWW in laboratory conditions.     

土壤微生物生物氮与植物氮吸收的关系

The contents of the soil microbial biomass nitrogen (SMBN) in the soils sampled from the Loess Plateau of China were determined using chloroform fumigation aerobic incubation method (CFAIM),chloroform fumigation anaerobic incubation method (CFANIM) and chloroform fumigation-extraction method (CFEM). The N taken up by ryegrass on the soils was determined after a galsshouse pot experiment. The flushes of nitrogen (FN) of the soils obtained by the CFAIM and CFANIM were higher than that by the CFEM, and there were significantly positive correlations between the FN obtained by the 3 methods. The N extracted from the fumigated soils by the CFAIM,CFANIM and CFEM were significantly positively correlated with the N uptake by ryegrass. The FN obtained by the 3 methods was also closely positively correlated with the N uptake by ryegrass. The FN obtained by the 3 methods was also closely positively correlated with the plant N uptake. The contributions of the SMBN and mineral N and mineralized N during the incubation period to plant N uptake were evaluated with the multiple regression method. The results showed that the N contained in the soil microbial biomass might play a noticeable role in the N supply of the soils to the plant.     

长期施肥对土壤微生物生物量碳、氮及矿质态氮含量动态变化的影响

利用位于陕西杨凌的17年长期定位试验研究了长期不施肥(CK)、单施化肥(F)、化肥配施有机肥(F+M)和化肥加秸秆还田(F+S)处理对小麦-玉米轮作体系中作物不同生长时期土壤微生物生物量碳、氮(SMBC、SMBN)和矿质态氮含量的影响。结果表明,0—10 cm土层土壤SMBC、SMBN和矿质态氮含量的变化范围分别为264.8~752.2、37.51~14.8和3.83~8.5 mg/kg。不同处理相比,F+M处理中各采样时期(小麦苗期、拔节期、灌浆期及玉米播种期、大喇叭口期、灌浆期和收获后)土壤SMBC和SMBN含量均为最高,分别为不施肥对照的1.382~.65和1.892~.50倍;F+S处理矿质态氮含量最高,SMBC和SMBN也高于F和CK处理,大部分采样时期的差异达显著水平(P0.05);与CK相比,长期单施化肥也使各时期SMBC和SMBN含量提高。在小麦拔节期到灌浆期的旺盛生长阶段各施肥处理土壤SMBN含量均下降,而矿质态氮含量变化不大,处于较低水平;在玉米大喇叭口期到灌浆期的旺盛生长阶段,F+M、F+S和F处理土壤矿质态氮含量显著下降,而SMBN含量均有所升高。表明在土壤矿质态氮含量较高时,作物首先利用矿质态氮,而在土壤矿质态氮含量处于较低水平时,微生物固持的氮素可能会释放出来供作物吸收利用。     

聚天冬氨酸尿素对土壤微生物量碳、氮的影响

土壤微生物量碳、氮是植物营养与土壤肥力的主要参数之一。采用对聚天冬氨酸、普通尿素及聚天冬氨酸尿素肥料培养实验、小麦盆栽方法,研究了普通尿素及聚天冬氨酸尿素对土壤微生物量碳、氮的影响。培养试验结果表明:聚天冬氨酸在培养早期能促进土壤微生物量碳、氮增加,聚天冬氨酸尿素处理比普通尿素处理对土壤微生物量碳、氮的作用更有效,前45 d,普通尿素处理的土壤微生物量氮降幅达21.87 mg.kg-1,而聚天冬氨酸尿素处理的土壤微生物量氮仅降低4.8 mg.kg-1;盆栽条件下,三叶期聚天冬氨酸尿素处理比普通尿素处理的土壤微生物量氮差值最高达12.57 mg.kg-1,成熟期差值为2.85 mg.kg-1,抽穗期、成熟期的聚天冬氨酸尿素处理比普通尿素处理土壤微生物量碳分别低16.12和14.81 mg.kg-1。说明聚天冬氨酸尿素的碱解氮易被聚天冬氨酸吸附,养分持效时间更长,比尿素具有一定增效作用。     

施氮量对土壤无机氮分布和微生物量氮含量及小麦产量的影响

在高肥力土壤条件下,研究了施氮量对土壤无机氮分布和微生物量氮含量及小麦产量的影响。结果表明,小麦生长期间,施氮处理0100.cm土层硝态氮积累量显著大于不施氮处理;当施氮量大于150.kg/hm2时,随施氮量增加,0100.cm土层硝态氮积累量显著增加;随小麦生育进程推进,施氮处理上层土壤硝态氮下移趋势明显,至小麦成熟时,施氮1952～85.kg/hm2处理60100.cm土层硝态氮含量显著大于其它处理。小麦生长期间,0100.cm土层铵态氮积累量较为稳定,施氮处理间亦无显著差异。与不施氮肥相比,施氮提高小麦生长期间040.cm土层土壤微生物量氮含量;当施氮量小于240.kg/hm2时,随施氮量增加,土壤微生物量氮含量增加。小麦的氮肥利用率随施氮量增加而降低;施氮1051～95.kg/hm2,收获时小麦植株吸氮量、生物产量、子粒产量和子粒蛋白质含量提高;而施氮量大于240.kg/hm2时,小麦生育后期的氮素积累量降低,收获时植株吸氮量、生物产量和子粒蛋白质含量降低。说明本试验条件下,施氮1051～50.kg/hm2可满足当季小麦氮素吸收利用,获得较高的子粒产量和蛋白质含量。继续增加施氮量,土壤微生物量氮含量增加,但土壤中残留大量硝态氮,易淋溶损失。     

聚丙烯酸盐类土壤改良剂对燕麦土壤微生物量氮及酶活性的影响

以燕麦田土壤为研究对象,探讨了聚丙烯酸盐类土壤改良剂及其复配(聚丙烯酸钾、聚丙烯酰胺、腐植酸钾、聚丙烯酸钾+腐植酸钾、聚丙烯酰胺+腐植酸钾)对燕麦田土壤微生物量氮及土壤酶活性的影响。结果表明,不同土壤改良剂均能提高土壤有机质、碱解氮、速效磷和速效钾的含量,各指标分别比对照增加了8.24%～30.22%、7.60%～19.29%、5.15%～29.45%和27.86%～68.86%;土壤改良剂能促使燕麦全生育期内0～10、10～20和20～40 cm各土层的土壤微生物量氮含量显著提高,聚丙烯酸钾+腐植酸钾和聚丙烯酰胺+腐植酸钾复配处理较其各单施效果显著,随土壤深度的增加土壤微生物量氮逐层递减;与对照相比,土壤改良剂能显著提高燕麦全生育期各土层过氧化氢酶活性,在抽穗期活性最高,且以聚丙烯酸钾+腐植酸钾较高;但对于脲酶,聚丙烯酸钾+腐植酸钾、聚丙烯酰胺+腐植酸钾和腐植酸钾3个处理在苗期显著低于对照,在抽穗期和成熟期高于对照,两种酶活性均随土壤深度的增加逐渐降低。     

Impact of soil fumigation practices on soil nematodes and microbial biomass

This study was designed to understand the impact of methyl bromide (MB) (CHaBr) and its alternatives on both free-living and root-knot nematodes in the soil. A randomized complete block experiment with six treatments and 4 replicates (each replicate in a separate greenhouse) was established in Qingzhou, Shandong Province, China. In addition to MB and untreated control (CK) treatments there were four alternative soil fumigation practices including MB virtually impermeable films (VIF), metam sodium (MS), MS VIF and soil solarization combined with selected biological control agents (SS BCA). Two tomato (Lycopersicum esculentum Mill.) cultivars, cv. Maofen-802 from the Xian Institute of Vegetable Science, China, and cv. AF179 Brillante from the Israeli Hazera Quality Seeds, were selected as test crops. The results indicated that Rhabditidae was the most dominant population with percentage abundance as high as 85% of the total number of identified free-living nematodes, followed by that of Cephalobidae. Methyl bromide and its alternatives except for the non-chemical SS BCA treatment controlled the target pest, root-knot nematodes. Also, the impact of the three chemical alternatives on free-living nematode number and functional group abundance was similar to the impact associated with a typical methyl bromide application. Chemical fumigation practices, especially that with MB, significantly reduced the number of nematodes in the soil and simultaneously significantly reduced the number of nematode genera thereby reducing nematode diversity. All the four soil chemical fumigation activities decreased soil microbial biomass and had an obvious initial impact on microorganism biomass. Furthermore, both plant-parasitic and fungivore nematodes were positively correlated with soil microbial biomass.     

Correlation among microbial biomass s,soil properties,and other biomass nutrients

The soil physicochemical characteristics and amounts of microbial biomass C, N, and S in 19 soils (10 grassland, 2 forest, and 7 arable soils) were investigated to clarify the S status in granitic regosols in Japan, in order to determine the relationships between biomass S and other soil characteristics and to estimate approximately the annual Sand N flux through the microbial biomass. Across the sites, the amount of biomass C ranged from 46 to 1,054, biomass N from 6 to 158, and biomass S from 0.81 to 13.44 mg kg_-1 soil with mean values of 438.8, 85.8, and 6.15 mg kg_-1 soil, respectively. Microbial biomass Nand S accounted for 3.4–7.7% and 1.1–4.0% of soil total Nand S, respectively. The biomass C: N, C : S, and N : S ratios varied considerably across the sites and ranged from 3.0–10.4, 32.5–87.7, and 5.0–18.8, respectively. Microbial biomass S was linearly related to biomass C and biomass N. The regression accounted for 96.6% for biomass C and 92.9% for biomass N of the variance in the data. The amounts of biomass C, N, and S were positively correlated with a number of soil properties, particularly with the contents of organic C, total N, SO₄-S, and electrical conductivity and among themselves. The soil properties, in various linear combinations showed a variability of 84–97% in the biomass nutrients. Stepwise multiple regression indicated that biomass C, N, and S were also dependent on SO₄-S as a second factor of significance which could limit microbial growth under the conditions prevailing at the study sites. Annual flux of Nand S was estimated through the biomass using the turnover rates of 0.67 for Nand 0.70 for S to be approximately 129 kg Nand 9.7 kg S ha^-1 y^-l, respectively, and was almost two times higher in grassland than arable soils.     

鸡粪与化肥不同配比对杨树苗根际土壤酶和微生物量碳、氮变化的影响

为比较鸡粪有机肥与化肥以不同比例配施对杨树苗根际土壤环境的作用效果,通过盆栽试验,研究了N100（尿素提供100%的氮）、 M10N90（鸡粪和尿素分别提供10%和90%的氮)、 M30N70（鸡粪和尿素分别提供30%和70%的氮）和M50N50（鸡粪和尿素各提供50%的氮）等处理对一年生杨树苗根际土壤酶活性、 微生物量碳（SMBC）和微生物量氮（SMBN）的影响。结果表明, 同N100处理相比,M30N70处理的杨树苗根际土壤脲酶、 碱性磷酸酶、 过氧化氢酶和蔗糖酶活性分别提高14.41%~26.49%、 13.87%~27.93%、 12.07%~27.08%和29.17%~46.43%; 根际SMBC、 SMBN含量分别提高63.00%~76.62%、 40.01%~90.38%。在杨树苗的年生长过程中,根际土壤脲酶、 碱性磷酸酶、 过氧化氢酶和蔗糖酶活性均在施肥后120 d 最高; 而根际SMBC和SMBN含量分别在施肥后90 d和30 d达到最大值。随着杨树苗的生长发育,SMBN含量逐渐降低,而在落叶期有一定的回升。与M30N70处理相比,M10N90和M50N50处理对杨树苗根际土壤酶和微生物量碳、 氮的影响较小。综合分析认为,鸡粪有机肥与化肥以3∶7比例配施对杨树苗根际区域生态环境的改善效果最佳。     

废弃农耕地自然演替过程中微生物群落与土壤特性间的关系

The changes of microbial biomass carbon (MBC) and nitrogen (MBN) and microbial community in the topsoil of the abandoned agricultural land on the semi-arid Loess Plateau in China during the natural succession were evaluated to understand the relationship between microbial community and soil properties. MBC and MBN were measured using fumigation extraction, and microbial community was analyzed by the method of fatty acid methyl ester (FAME). The contents of organic C, total N, MBC, MBN, total FAME, fungal FAME, bacterial FAME and Gram-negative bacterial FAME at the natural succession sites were higher than those of the agricultural land, but lower than those of the natural vegetation sites. The MBC, MBN and total FAME were closely correlated with organic C and total N. Furthermore, organic C and total N were found to be positively correlated with fungal FAME, bacterial FAME, fungal/bacterial and Gram-negative bacterial FAME. Natural succession would be useful for improving soil microbial properties and might be an important alternative for sustaining soil quality on the semi-arid Loess Plateau in China.     

Response of white mustard (Sinapis alba) and the soil microbial biomass to P and Zn addition in a greenhouse pot experiment

A 45‐d pot experiment was carried out to investigate the response of white mustard and the soil microbial biomass after Zn and P addition to a P deficient silt loam. The underlying hypothesis was that P application reduces the Zn availability to crops and microbial biomass. White mustard was supplied with different levels of P (0, 50, and 100 µg g^?1 soil) and Zn (0, 10, and 20 µg g^?1 soil). Amendments of P generally reduced extractable Zn, shoot Zn and soil microbial biomass Zn. Amendments of P generally decreased the microbial biomass C/P ratio. At 20 µg Zn g^?1 soil, a negative effect on the microbial biomass C/P ratio was observed, suggesting that high contents of extractable Zn have a negative impact on the microbial P uptake. However, the minimum Zn requirements of soil microorganisms and the consequences of microbial Zn deficiency for soil microbiological processes are completely unknown.     

不同栽培方式对温室连作黄瓜土壤微生物量碳氮和作物产量的影响

以连作8年温室连作黄瓜土壤为材料,试验设5个不同轮作方式处理, 1）早春茬黄瓜夏茬休闲秋冬茬黄瓜（CK）; 2）早春茬黄瓜夏茬休闲秋冬茬黄瓜间作大蒜（CS1）; 3）早春茬黄瓜夏茬茼蒿秋冬茬黄瓜（CS2）; 4）早春茬番茄夏茬菠菜秋冬茬黄瓜（CS3）; 5）早春茬黄瓜夏茬大蒜秋冬茬黄瓜(CS4),进行了5年的盆栽试验,采用后3年的数据,评价不同栽培方式对土壤微生物量碳氮和主栽作物产量的连年影响,探讨栽培方式对连作黄瓜土壤的修复效果。研究结果表明,相对CK,CS3在第三和四年早春茬显著增加微生物量碳、 氮以及碳/氮,CS2和CS3在三至五年夏茬显著增加微生物量碳和碳/氮, CS2显著增加三至五年早春茬、 早春茬+秋冬茬产量,CS4显著增加第3和4年早春茬、 早春茬+秋冬茬产量和第三至五年秋冬茬产量; 微生物量碳和碳/氮与秋冬茬产量呈显著正相关,与早春茬+秋冬茬产量呈极显著正相关; 处理与季节均对微生物量碳和碳/氮产生极显著影响,且两者有极显著交互作用; 季节、 处理季节对微生物量氮有显著影响,处理、 季节、 处理季节对作物产量有极显著影响。总之,CS2提高微生物量碳、 碳/氮、 作物产量效果最佳。     

Relationship between soil chemical properties and microbial metabolic patterns in intensive greenhouse tomato production systems

ABSTRACT

Intensive greenhouse production involving excessive fertilizer and organic manure application rates may affect soil chemical and biological quality. Soil samples from 50 commercial greenhouses for tomato production in northern China were collected for the evaluation of the status of soil fertility and identification of the soil chemical factor that exerts the strongest influence on microbial functional diversity. The soil total nitrogen content showed high soil fertility and was 68% higher than 1000 mg kg^?1 and 14% higher than 1500 mg kg^?1. Differential soil pH values caused statistically significant shifts in microbial metabolic activity (average well color development, AWCD) and Shannon’s diversity index using Biolog^TM ECO plates assay. The highest soil microbial functional diversity was observed at near neutral pH values. When individual data points were plotted against soil organic matter (SOM), significant positive associations with soil microbial biomass nitrogen and AWCD were observed. The canonical correspondence analysis confirmed that shifts in the soil microbial functional diversity were associated with changes in pH, total nitrogen, and SOM. This study indicated that excessive fertilization changed the community-level physiological profile of the soil microorganisms, and this effect can be a consequence of changes in soil pH under intensive greenhouse management.     

连年翻压绿肥对植烟土壤微生物量及酶活性的影响

通过3年田间定位试验,研究连年翻压绿肥对植烟土壤微生物量碳、氮及酶活性的影响。结果表明,连年翻压绿肥能提高土壤微生物量碳、氮及土壤脲酶、酸性磷酸酶、蔗糖酶、过氧化氢酶的活性,且随翻压年限的增加而增加。整个生育期,翻压3年绿肥的处理与对照相比微生物量碳、氮分别提高31.0%~67.1%、23.0%~145.1%;土壤脲酶、酸性磷酸酶、蔗糖酶、过氧化氢酶活性分别提高34.4%~51.9%、11.0%~18.6%、58.0%~172.7%、24.0%~50.0%,表明翻压绿肥后土壤生物过程活跃,利于有机物质的转化和烤烟正常生长所需的营养供应。动态变化特征表明,翻压绿肥1、2、3年的各处理微生物量碳、氮均在团棵期出现峰值,土壤脲酶、酸性磷酸酶、过氧化氢酶均在旺长期出现峰值。在出现峰值时翻压3年的处理与对照相比微生物量碳、氮分别提高67.1%、60.7%;土壤脲酶、酸性磷酸酶、过氧化氢酶活性分别提高51.9%、14.2%、30.6%。此时正值生育旺期,利于烟株生长发育,说明连年翻压绿肥后培肥土壤效果显著。土壤微生物量C、N和酶活性能灵敏反映土壤肥力的变化,可作为评价土壤质量的生物学指标。     

长期稻秆还田对土壤微生物量及C、N动力学的影响

A study was performed on the long-term effect of straw incorporation on soil microbial biomass C contents, C and N dynamics in both Rothamsted and Woburn soils. The results showed that for both soils, the microbial biomass C contents were significantly different among all the treatments, and followed the sequence in treatments of straw chopped and incorporated into 10 cm (CI10) > straw burnt and incorporated into 10 cm (BI10) > straw chopped and incorporated into 20 cm (CI20) > straw burnt and incorporated into 20 cm (BI20). Laboratory incubation of soils showed that the cumulative CO₂ evolution was closely related to the soil microbial biomass C content. Carbon dioxide evolution rates (CO₂-C, μg (g•d)^-1) decreased rapidly in the first two weeks' incubation, then decreased more slowly. The initial K₂SO₄-extractable NH₄-N and NO₃-N contents were low and similar in all the treatments, and all increased gradually with the incubation time. However, net N immobilization was observed in chopped treatments for Rothamsted soils during the first 4 weeks. Nevertheless, more N mineralization occurred in Treatment CI10 than any other treatment at the end of incubation for both soils. The Woburn soils could more easily suffer from the leaching of nitrate because the soils were more permeable and more N was mineralized during the incubation compared to the Rothamsted soils.     

Effect of indaziflam on microbial activity and nitrogen cycling processes in an orchard soil

Indaziflam is a preemergent herbicide widely used for the control of weeds in pecan (Carya illinoinensis) orchards in the southwestern region of the United States. Given the paucity of data regarding the effect of indaziflam on the biochemical properties of soils supporting pecan production, this study was conducted to evaluate the effects of different application rates of indaziflam on soil microbial activity, diversity, and biochemical processes related to nitrogen (N) cycling. During two consecutive growing seasons (2015 and 2016), soil samples were obtained from experimental mesocosms consisting of soil-filled pots where pecan saplings were grown and treated with indaziflam applied at two different rates (25 and 50 g active ingredient (ai) ha^-1, with the higher rate being slightly lower than the recommended field application rate of 73.1 g ai ha^-1). Soil samples were collected approximately one week before and one week after herbicide application for determination of soil microbial biomass and diversity, N mineralization, and β-glucosaminidase activity. Soil samples collected from the control mesocosms without herbicide application were treated in the laboratory with two rates of indaziflam (75 and 150 g ai ha^-1) to determine the immediate effect on microbial activity. No significant effect of herbicide treatment on soil respiration and microbial biomass was detected. The results showed a slight to moderate decrease in microbial diversity (7% in 2015 and 44% in 2016). However, decreased β-glucosaminidase activity with herbicide treatment was observed in soils from the mesocosms (33%) and soils treated with indaziflam in the laboratory (45%). The mineral N pool was generally dominated by ammonium after indaziflam application, which was consistent with the drastic decrease (75%) in nitrification activity measured in the laboratory experiment. The results of this study indicate that indaziflam, even when applied at higher than recommended rates, has limited effects on soil microbial activity, but may affect N cycling processes.     

黄土高原某些耕作土壤中土壤微生物生物量C、N与矿化N的关系

The chloroform fumigation-incubation method was used to measure the soil microbial biomass C (SMBC) and N (SMBN) in 16 loessial soils sampled from Ansai, Yongshou and Yangling in Shaanxi Province. The SMBC contents in the soils ranged from 75.9 to 301.0 μg C g^-1 with an average of 206.1 μg C g^-1, accounting for 1.36%~6.24% of the total soil organic C with an average of 3.07%, and the SMBN contents from 0.51 to 68.40 μg N g^-1 with an average of 29.4 μg N g^-1, accounting for 0.20%~5.65% of the total N in the soils with an average of 3.36%. A close relationship was found between SMBC and SMBN, and they both were positively correlated with total organic C, total N, NaOH hydrolizable N and mineralizable N. These results confirmed that soil microbial biomass had a comparative role in nutrient cycles of soils.