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1.
The impacts of crop rotations and N fertilization on different pools of urease activity were studied in soils of two long-term
field experiments in Iowa; at the Northeast Research Center (NERC) and the Clarion-Webster Research Center (CWRC). Surface
soil samples (0–15 cm) were taken in 1996 and 1997 in corn, soybeans, oats, or meadow (alfalfa) plots that received 0 or 180 kg
N ha–1, applied as urea before corn and an annual application of 20 kg P and 56 kg K ha–1. The urease activity in the soils was assayed at optimal pH (THAM buffer, pH 9.0), with and without toluene treatment, in
a chloroform-fumigated sample and its nonfumigated counterpart. The microbial biomass C (Cmic) and N (Nmic) were determined by chloroform fumigation methods. The total, intracellular, extracellular and specific urease activities
in the soils of the NERC site were significantly affected by crop rotation, but not by N fertilization. Generally, the highest
total urease activities were obtained in soils under 4-year oats–meadow rotations and the lowest under continuous corn. The
higher total activities under multicropping systems were caused by a higher activity of both the intracellular and extracellular
urease fractions. In contrast, the highest values for the specific urease activity, i.e. of urease activity of the microbial
biomass, were found in soils under continuous soybean and the least under the 4-year rotations. Total and extracellular urease
activities were significantly correlated with Cmic (r>0.30* and >0.40**) and Nmic (r>0.39** and >0.44**) in soils of the NERC and CWRC sites, respectively. Total urease activity was significantly correlated
with the intracellular activity (r>0.73***). About 46% of the total urease activity of the soils was associated with the microbial biomass, and 54% was extracellular
in nature.
Received: 25 May 1999 相似文献
2.
The impacts of crop rotations and N fertilization on microbial biomass C (Cmic) and N (Nmic) were studied in soils of two long-term field experiments initiated in 1978 at the Northeast Research Center (NERC) and in
1954 at the Clarion-Webster Research Center (CWRC), both in Iowa. Surface soil samples were taken in 1996 and 1997 from plots
of corn (Zea mays L.), soybeans (Glycine max (L.) Merr.), oats (Avena sativa L.), or meadow (alfalfa) (Medicago sativa L.) that had received 0 or 180 kg N ha–1 before corn and an annual application of 20 kg P and 56 kg K ha–1. The Cmic and Nmic values were determined by the chloroform-fumigation-extraction method and the chloroform-fumigation-incubation method, respectively.
The Cmic and Nmic values were significantly affected (P<0.05) by crop rotation and plant cover at time of sampling, but not by N fertilization. In general, the highest Cmic and Nmic contents were found in the multicropping systems (4-year rotations) taken in oats or meadow plots, and the lowest values
were found in continuous corn and soybean systems. On average, Cmic made up about 1.0% of the organic C (Corg), and Nmic contributed about 2.4% of the total N (Ntot) in soils at both sites and years of sampling. The Cmic values were significantly correlated with Corg contents (r≥0.41**), whereas the relationship between Cmic and Ntot was significant (r≤0.53***) only for the samples taken in 1996 at the NERC site. The Cmic : Nmic ratios were, on average, 4.3 and 6.4 in 1996, and 7.6 and 11.4 in 1997 at the NERC and CWRC sites, respectively. Crop rotation
significantly (P<0.05) affected this ratio only at the NERC site, and N fertilization showed no effect at either site. In general, multicropping
systems resulted in greater Cmic : Corg (1.1%) and Nmic : Ntot (2.6%) ratios than monocropping systems (0.8% and 2.1%, respectively).
Received: 9 February 1999 相似文献
3.
Glycosidases are a group of soil enzymes that play a major role in degradation of carbohydrates. This study was conducted to assess the impact of crop rotation and N fertilization on the activities of α‐ and β‐glucosidases and α‐ and β‐galactosidases in plots of two long‐term field experiments at the Clarion‐Webster Research Center (CWRC) and Northeast Research Center (NERC) in Iowa. Surface‐soil (0–15 cm) samples were taken in 1996 and 1997 in corn (Zea mays L.), soybean (Glycine max (L.) Merr.), oats (Avena sativa L.), or meadow (alfalfa) (Medicago sativa L.) plots that received 0 or 180 kg N ha–1, applied as urea before corn, and an annual application of 20 kg P ha–1 and 56 kg K ha–1. Activities of the four glycosidases were significantly affected by crop rotations in both years at the two sites but not by nitrogen application. In general, higher activities were observed in plots under meadow or oat and the lowest in continuous corn (CWRC) and soybean (NERC). Four‐year rotation showed the highest activity, followed by 2‐year rotation and monocropping systems. Linear‐regression analyses indicated that, in general, the activities of the glycosidases were significantly correlated with microbial‐biomass C (r > 0.302, p ≤ 0.05) and microbial‐biomass N (r > 0.321, p ≤ 0.05), organic‐C (r > 0.332, p ≤ 0.05) and organic‐N (r > 0.399, p ≤ 0.01) contents of the soils. Results of this work suggest that multicropping stimulated the activities of the glycosidases. The specific activities of the glycosidases in soils of the two sites studied, expressed as g p‐nitrophenol released per kg of organic C, differed among the four enzymes. The lowest values were obtained for β‐galactosidase and α‐glucosidase, followed by α‐galactosidase and β‐glucosidase. 相似文献
4.
The relationships between arylsulfatase and microbial activity were investigated in regional and microenvironmental scales, at three study sites in Israel, that represent different climatic regions—Mediterranean (sub-humid), mildly arid and arid.Total arylsulfatase activity was divided into extracellular and intracellular (microbial biomass enzyme) activities according to the chloroform-fumigation method. The results show that with increasing aridity, Corg (soil organic carbon), Cmic (soil microbial biomass carbon), Nmic (soil microbial biomass nitrogen) and respiration rate decreased, while Cmic/Corg and metabolic quotient (qCO2) increased. Total, extracellular and microbial biomass arylsulfatase activities decreased with aridity. Expressed as percentage of total activity, the arylsulfatase activity of microbial biomass in the soil, at 0-2 cm and 5-10 cm depths, accounted for more than 50% of the total, in most measurements. This activity was significantly higher in the arid sites than that found in the Mediterranean one for the 0-2 cm soil. The results indicate the importance of the microflora as an enzyme source in soils, especially in arid climate conditions.Enzyme activity in the different study sites was found to be influenced by microenvironmental conditions. The Mediterranean site showed a much higher enzyme activity under shrubs than that under rock fragments and in bare soil. In the arid site rock fragments created a favorable microenvironment for microbial activity on soil surface, which resulted in a much higher microbial biomass and arylsulfatase activity than that in bare soil.The total, extracellular and intracellular arylsulfatase activities, were significantly correlated with Corg, Cmic, Nmic and respiration rate (p<0.05) at all study sites. The correlation coefficients between microbial biomass and arylsulfatase activity were usually higher than those between organic carbon and enzyme activity, especially in the arid sites. Close relationships between microbial biomass and arylsulfatase activities in all the studied sites supported the hypothesis that Corg content and enzyme activities should be related to each other via microbial biomass. Arylsulfatase activity was found to be a good indicator of microbial one. The regression equations between these factors can be incorporated into models of biogeochemical cycling for their easy method of analysis. 相似文献
5.
Effect of cropping systems on phosphatases in soils 总被引:2,自引:0,他引:2
Phosphatases are widely distributed in nature and play a major role in phosphorus nutrition of plants. The effects of crop rotations and nitrogen fertilization on the activities of phosphatases (acid phosphatase, alkaline phosphatase, and phosphodiesterase) were studied in soils from two long‐term cropping systems at the Northeast Research Center (NERC) in Nashua and the Clarion Webster Research Center (CWRC) in Kanawha, Iowa, USA. Surface soils (0—15 cm) were taken in 1996 and 1997 from replicated field plots in corn, soybeans, oats, or meadow (alfalfa) that received 0 or 180 kg N ha—1 before corn. Because of differences in organic C contents among soils of the two sites, the soils from the CWRC sites contained greater enzyme activity values than those from the NERC site. Plots under oats or meadow showed the greatest activity values, whereas those under continuous corn at the CWRC site and soybean at the NERC site showed the least activities. Analysis of variance indicated that the activities of the phosphatases were significantly affected by crop rotation (P < 0.001) in both years at the NERC site but not at the CWRC site. Nitrogen fertilization affected the activity of acid phosphatase in soils from the CWRC site in both years and alkaline phosphatase only in 1997; but it did not affect the activities of the phosphatases in the soils from the NERC site. With the exception of alkaline phosphatase (CWRC) and phosphodiesterase (NERC) in soils sampled in 1997, activities of alkaline phosphatase and phosphodiesterase were significantly correlated with microbial biomass C (C mic) in soils from both sites and years, with r values ranging from 0.366* to 0.599***. Cropping systems and N fertilization affected the specific activities of phosphomonoesterases, especially acid phosphatase, but not of phosphodiesterase. Regression analysis showed that activities of phosphatases were significantly correlated with organic C contents of soils from the NERC site but not from the CWRC site. 相似文献
6.
Effect of cropping systems on nitrogen mineralization in soils 总被引:3,自引:0,他引:3
Understanding the effect of cropping systems on N mineralization in soils is crucial for a better assessment of N fertilizer
requirements of crops in order to minimize nitrate contamination of surface and groundwater resources. The effects of crop
rotations and N fertilization on N mineralization were studied in soils from two long-term field experiments at the Northeast
Research Center and the Clarion-Webster Research Center in Iowa that were initiated in 1979 and 1954, respectively. Surface
soil samples were taken in 1996 from plots of corn (Zea mays L.), soybean (Glycine max (L.) Merr.), oats (Avena sativa L.), or meadow (alfalfa) (Medicago sativa L.) that had received 0 or 180 kg N ha–1 before corn and an annual application of 20 kg P and 56 kg K ha–1. N mineralization was studied in leaching columns under aerobic conditions at 30 °C for 24 weeks. The results showed that
N mineralization was affected by cover crop at the time of sampling. Continuous soybean decreased, whereas inclusion of meadow
increased, the amount of cumulative N mineralized. The mineralizable N pool (N
o) varied considerably among the soil samples studied, ranging from 137 mg N kg–1 soil under continuous soybean to >500 mg N kg–1 soil under meadow-based rotations, sampled in meadow. The results suggest that the N
o and/or organic N in soils under meadow-based cropping systems contained a higher proportion of active N fractions.
Received: 10 February 1999 相似文献
7.
Studies were conducted to evaluate the relationships among different active N pools of organic matter in soils at two long-term
cropping systems in Iowa. Results indicated that multi-cropping systems, particularly meadow-based systems, enhanced bioactivities
of soils. Mono-cropping systems, particularly soybean, reduced soil microbial biomass and enzyme activities. The mineralizable
N pool (potential N mineralization;N
o) was more sensitive to changes in the size of the microbial biomass N (Nmic) than to changes in organic N. One unit change in organic N did not lead to substantial changes in N
o, but 1 unit change in Nmic resulted in three or more units change in N
o. The active N pools and turnover rate were more sensitive to changes in organic C than to changes in microbial biomass C
(Cmic). A unit change in organic C resulted in 10.6 units change in N
o, but a unit change in Cmic resulted in only 0.8 unit change in N
o. Cmic or Nmic are better indexes than organic C or N for the estimation of N
o or N availability, because biomass values are more highly correlated with cumulative N mineralized during 24 weeks of incubation,
with r values ranging from 0.57 (P<0.001) to 0.88 (P<0.001).
Received: 18 October 1999 相似文献
8.
Soil microbial biomass and nitrogen supply in an irrigated lowland rice soil as affected by crop rotation and residue management 总被引:5,自引:0,他引:5
C. Witt Kenneth G. Cassman Johannes C. G. Ottow Ulrich Biker 《Biology and Fertility of Soils》1998,28(1):71-80
Processes that govern the soil nitrogen (N) supply in irrigated lowland rice systems are poorly understood. The objectives
of this paper were to investigate the effects of crop rotation and management on soil N dynamics, microbial biomass C (CBIO) and microbial biomass N (NBIO) in relation to rice N uptake and yield. A maize-rice (M-R) rotation was compared with a rice-rice (R-R) double-cropping
system over a 2-year period with four cropping seasons. In the M-R system, maize (Zea mays L.) was grown in aerated soil during the dry season (DS) followed by rice (Oryza sativa L.) grown in flooded soil during the wet season (WS). In the R-R system, rice was grown in flooded soil in both the DS and
WS. Three fertilizer N rates (0, 50 or 100 kg urea-N ha–1 in WS) were assigned to subplots within the cropping system main plots. Early versus late crop residue incorporation following
DS maize or rice were established as additional treatments in sub-subplots in the second year. In the R-R system, the time
of residue incorporation had a large effect on NO3
–-N accumulation during the fallow period and also on extractable NH4
+-N, rice N uptake and yield in the subsequent cropping period. In contrast, time of residue incorporation had little influence
on extractable N in both the fallow and rice-cropping periods of the M-R system, and no detectable effects on rice N uptake
or yield. In both cropping systems, CBIO and NBIO were not sensitive to residue incorporation despite differences of 2- to 3-fold increase in the amount of incorporated residue
C and N, and were relatively insensitive to N fertilizer application. Extractable organic N was consistently greater after
mid-tillering in M-R compared to the R-R system across N rate and residue incorporation treatments, and much of this organic
N was α-amino N. We conclude that N mineralization-immobilization dynamics in lowland rice systems are sensitive to soil aeration
as influenced by residue management in the fallow period and crop rotation, and that these factors have agronomically significant
effects on rice N uptake and yield. Microbial biomass measurements, however, were a poor indicator of these dynamics.
Received: 31 October 1997 相似文献
9.
Ratios between estimates of microbial biomass content and microbial activity in soils 总被引:10,自引:0,他引:10
The content levels and activities of the microbiota were estimated in topsoils and in one soil profile at agricultural and forest sites of the Bornhöved Lake district in northern Germany. Discrepancies between data achieved by fumigation-extraction (FE) and substrate-induced respiration (SIR), both used for the quantification of microbial biomass, were attributed to the composition of the microbial populations in the soils. In the topsoils, the active, glucose-responsive (SIR) versus the total, chloroform-sensitive microbial (FE) biomass decreased in the order; field maize monoculture (field-MM)>field crop rotation (field-CR) and dry grassland>beech forest. This ratio decreased within the soil profile of the beech forest from the litter horizon down to the topsoil. Differences between microbial biomass and activities suggested varying biomass-specific transformation intensities in the soils. The metabolic quotient (qCO2), defined as the respiration rate per unit of biomass, indicates the efficiency in acquiring organic C and the intensity of C mineralization, while biomass-specific arginine-ammonification (arginine-ammonification rate related to microbial biomass content) seems to be dependent on N availability. The qCO2, calculated on the basis of the total microbial biomass, decreased for the topsoils in the same order as did the ratio between the active, glucose-responsive microbial biomass to the total, chloroform-sensitive microbial biomass, in contrast to qCO2 values based on the glucose-responsive microbial biomass, which did not. There was no difference between the levels of biomass-specific arginine-ammonification in topsoils of the fertilized field-CR, fertilized field-MM, fertilized dry grassland and eutric alder forest, but levels were lower in the beech forest, dystric alder forest, and unfertilized wet grassland topsoils. Ratios between values of different microbiological features are suggested to be more useful than microbiological features related to soil weight when evaluating microbial populations and microbially mediated processes in soils. 相似文献
10.
11.
Biological and chemical properties of arable soils affected by long-term organic and inorganic fertilizer applications 总被引:17,自引:0,他引:17
M. Šimek D. W. Hopkins J. Kalčík T. Picek H. Šantrůčková J. Staňa K. Trávník 《Biology and Fertility of Soils》1999,29(3):300-308
Using soils from field plots in four different arable crop experiments that have received combinations of manure, lime and
inorganic N, P and K for up to 20 years, the effects of these fertilizers on soil chemical properties and estimates of soil
microbial community size and activity were studied. The soil pH was increased or unaffected by the addition of organic manure
plus inorganic fertilizers applied in conjunction with lime, but decreased in the absence of liming. The soil C and N contents
were greater for all fertilized treatments compared to the control, yet in all cases the soil samples from fertilized plots
had smaller C:N ratios than soil from the unfertilized plots. The soil concentrations of all the other inorganic nutrients
measured were greater following fertilizer applications compared with the unfertilized plots, and this effect was most marked
for P and K in soils from plots that had received the largest amounts of these nutrients as fertilizers. Both biomass C determined
by chloroform fumigation and glucose-induced respiration tended to increase as a result of manure and inorganic fertilizer
applications, although soils which received the largest additions of inorganic fertilizers in the absence of lime contained
less biomass C than those to which lime had been added. Dehydrogenase activity was lower in soils that had received the largest
amounts of fertilizers, and was further decreased in the absence of lime. This suggests that dehydrogenase activity was highly
sensitive to the inhibitory effects associated with large fertilizer additions. Potential denitrification and anaerobic respiration
determined in one soil were increased by fertilizer application but, as with both the microbial biomass and dehydrogenase
activity, there were significant reductions in both N2O and CO2 production in soils which received the largest additions of inorganic fertilizers in the absence of lime. In contrast, the
size of the denitrifying component of the soil microbial community, as indicated by denitrifying enzyme activity, was unaffected
by the absence of lime at the largest rate of inorganic fertilizer applications. The results indicated differences in the
composition or function of microbial communities in the soils in response to long-term organic and inorganic fertilization,
especially when the soils were not limited.
Received: 10 March 1998 相似文献
12.
A rapid chloroform-fumigation extraction method for measuring soil microbial biomass carbon and nitrogen in flooded rice soils 总被引:2,自引:0,他引:2
C. Witt J. L. Gaunt C. C. Galicia J. C. G. Ottow H.-U. Neue 《Biology and Fertility of Soils》2000,30(5-6):510-519
A chloroform-fumigation extraction method with fumigation at atmospheric pressure (CFAP, without vacuum) was developed for
measuring microbial biomass C (CBIO) and N (NBIO) in water-saturated rice soils. The method was tested in a series of laboratory experiments and compared with the standard
chloroform-fumigation extraction (CFE, with vacuum). For both methods, there was little interference from living rice roots
or changing soil water content (0.44–0.55 kg kg–1 wet soil). A comparison of the two techniques showed a highly significant correlation for both CBIO and NBIO (P<0.001) suggesting that the simple and rapid CFAP is a reliable alternative to the CFE. It appeared, however, that a small
and relatively constant fraction of well-protected microbial biomass may only be lysed during fumigation under vacuum. Determinations
of microbial C and N were highly reproducible for both methods, but neither fumigation technique generated NBIO values which were positively correlated with CBIO. The range of observed microbial C:N ratios of 4–15 was unexpectedly wide for anaerobic soil conditions. Evidence that this
was related to inconsistencies in the release, degradation, and extractability of NBIO rather than CBIO came from the observation that increasing the fumigation time from 4 h to 48 h significantly increased NBIO but not CBIO. The release pattern of CBIO indicated that the standard fumigation time of 24 h is applicable to water-saturated rice soils. To correct for the incomplete
recovery of CBIO, we suggest applying the k
C factor of 2.64, commonly used for aerobic soils (Vance et al. 1987), but caution is required when correcting NBIO data. Until differences in fumigation efficiencies among CFE and CFAP are confirmed for a wider range of rice soils, we suggest
applying the same correction factor for both methods.
Received: 1 June 1999 相似文献
13.
Organic matter, microbial biomass and enzyme activity of soils under different crop rotations in the tropics 总被引:8,自引:0,他引:8
Soil organic matter level, soil microbial biomass C, ninhydrin-N, C mineralization, and dehydrogenase and alkaline phosphatase
activity were studied in soils under different crop rotations for 6 years. Inclusion of a green manure crop of Sesbania aculeata in the rotation improved soil organic matter status and led to an increase in soil microbial biomass, soil enzyme activity
and soil respiratory activity. Microbial biomass C increased from 192 mg kg–1 soil in a pearl millet-wheat-fallow rotation to 256 mg kg–1 soil in a pearl millet-wheat-green manure rotation. Inclusion of an oilseed crop such as sunflower or mustard led to a decrease
in soil microbial biomass, C mineralization and soil enzyme activity. There was a good correlation between microbial biomass
C, ninhydrin-N and dehydrogenase activity. The alkaline phosphatase activity of the soil under different crop rotations was
little affected. The results indicate the green manuring improved the organic matter status of the soil and soil microbial
activity vital for the nutrient turnover and long-term productivity of the soil.
Received: 7 January 1996 相似文献
14.
Microwave irradiation was evaluated as a non-toxic alternate to chloroform fumigation for routine measurement of soil microbial
biomass C. Microwave energy was applied to moist soil to disrupt microbial cells. The flush of C released was then measured
after extraction or incubation. Microwave irradiation at 800 J g–1 soil was optimal because this level resulted in an almost instantaneous rise in soil temperature (≥80 °C), an abrupt reduction
in microbial activity, maximal release of biomass C, and minimal solubilization of humic substances. Both incubation-CO2 titration and extraction-colorimetry methods were used on separate 20-g subsamples to compare the labile C in the microwave-treated
and untreated soil samples. The incubation-titration method was also used to measure C in chloroform-fumigated soil samples.
Averaged across soils, the chloroform fumigation yielded 123.3±5.1 mg CO2-C kg–1. Microwave irradiation yielded 93.6±3.9 mg CO2-C kg–1 soil determined by incubation and 52.4±2.4 mg C kg–1 soil determined by extraction, accounting for 76% and 42% of the net flush of C measured by the chloroform fumigation. Microwave-stimulated
net flushes of C were correlated closely (r
2=0.974 for incubation or 0.908 for extraction) with microbial biomass C measured by the chloroform fumigation. Little correlation
was found with the total soil organic C (r
2=0.241 for incubation or for 0.166 extraction). Mean efficiency factors for incubation (K
MI) or extraction (K
ME) were used to calculate microbial biomass C from net flushes of C between microwaved and unmicrowaved soils. Values of K
MI and K
ME were not affected by soil pH, bulk density or clay contents. Extraction of microwaved soil by 0.5M K2SO4 proved to be a simple, fast, precise, reliable, and safe method to measure soil microbial biomass C.
Received: 12 September 1997 相似文献
15.
Chemical and biological characteristics of alkaline saline soils from the former Lake Texcoco as affected by artificial drainage 总被引:3,自引:0,他引:3
M. L. Luna-Guido R. I. Beltrán-Hernández N.A. Solís-Ceballos N. Hernández-Chávez F. Mercado-García J. A. Catt V. Olalde-Portugal L. Dendooven 《Biology and Fertility of Soils》2000,32(2):102-108
Soils from the former Lake Texcoco are alkaline saline and were artificially drained and irrigated with sewage effluents
since the late 1980s. Undrained soil and soil drained for 1, 5 and 8 years were sampled, characterized and incubated aerobically
for 90 days at 22±1 °C while production of CO2, available P and concentrations of NH4
+, NO2
– and NO3
– were monitored. Artificial drainage decreased pHH2O, water holding capacity, organic C, total N, and Na+, K+, Mg2+, B, Cl– and SO4
2– concentrations, increased inorganic C and Ca2+ concentrations more than 5-fold while total P was not affected. Microbial biomass C decreased with increased length of drainage
but bacteria, actinomycetes, denitrifiers and cellulose-utilizing bacteria tended to show opposite trends. CO2 production was less in soils drained ≥5 years compared to undrained soil but more than in soils drained for 1 year. Emission
of NH3 was negligible and concentrations of NH4
+ remained constant over time in each soil. Nitrification, as witnessed by increases in NO3
– concentrations, occurred in soil drained for 8 years. NO2
– concentrations decreased in soils drained ≤1 year in the first 7 days of the incubation and remained constant thereafter.
It was found that artificial drainage of soils from the former Lake Texcoco profoundly affected soil characteristics. Decreases
in pH and Na+, K+, Cl– and SO4
2– concentrations made conditions more favourable for plant growth, although low concentrations of inorganic N and available
P might be limiting factors.
Received: 1 December 1999 相似文献
16.
In a cropping systems experiment in southeastern Norway, ecological (ECO), integrated (INT) and conventional (CON) forage
(FORAGE) and arable (ARABLE) model farms were compared. After 5 experimental years, topsoil was sampled in spring from spring
grain plots and incubated for 449 days at controlled temperature (15 °C) and moisture content (50% water-holding capacity).
There were no detectable differences between model farms in terms of total soil C or N. For INT and CON, however, values of
microbial biomass C and N, microbial quotient (Cmic/Corg), and C and N mineralization were, or tended to be, higher for FORAGE than for ARABLE. For the ECO treatment, values were
similar for FORAGE and ARABLE and did not differ significantly from that of CON-FORAGE. For INT and CON, the metabolic quotient
(qCO2) was lower for FORAGE than for ARABLE. Again, for the ECO treatment, values were similar for FORAGE and ARABLE and did not
differ significantly from that of CON-FORAGE. We estimated the sizes of conceptual soil organic matter pools by fitting a
decomposition model to biomass and mineralization data. This resulted in a 48% larger estimate for CON-FORAGE than for CON-ARABLE
of physically protected biomass C. For physically protected organic C the difference was 42%. Moreover, the stability of soil
aggregates against artificial rainfall was substantially greater for CON-FORAGE than for CON-ARABLE. On this basis, we hypothesized
that the lower qCO2 values in the FORAGE soils were mainly caused by a smaller proportion of active biomass due to enclosure of microorganisms
within aggregates. Altogether, our results indicated a poorer inherent soil fertility in ARABLE than in FORAGE rotations,
but the difference was small or absent in the ECO system, probably owing to the use of animal and green manures and reduced
tillage intensity in the ECO-ARABLE rotation.
Received: 28 October 1998 相似文献
17.
Understanding the influence of long-term crop management practices on the soil microbial community is critical for linking soil microbial flora with ecosystem processes such as those involved in soil carbon cycling. In this study, pyrosequencing and a functional gene array (GeoChip 4.0) were used to investigate the shifts in microbial composition and functional gene structure in a medium clay soil subjected to various cropping regimes. Pyrosequencing analysis showed that the community structure (β-diversity) for bacteria and fungi was significantly impacted among different cropping treatments. Functional gene array-based analysis revealed that crop rotation practices changed the structure and abundance of genes involved in C degradation. Significant correlations were observed between the activities of four enzymes involved in soil C degradation and the abundance of genes responsible for the production of respective enzymes, suggesting that a shift in the microbial community may influence soil C dynamics. We further integrated physical, chemical, and molecular techniques (qPCR) to assess relationships between soil C, microbial derived enzymes and soil bacterial community structure at the soil micro-environmental scale (e.g. within different aggregate-size fractions). We observed a dominance of different bacterial phyla within soil microenvironments which was correlated with the amount of C in the soil aggregates suggesting that each aggregate represents a different ecological niche for microbial colonization. Significant effects of aggregate size were found for the activity of enzymes involved in C degradation suggesting that aggregate size distribution influenced C availability. The influence of cropping regimes on microbial and soil C responses declined with decreasing size of soil aggregates and especially with silt and clay micro-aggregates. Our results suggest that long term crop management practices influence the structural and functional potential of soil microbial communities and the impact of crop rotations on soil C turnover varies between different sized soil aggregates. These findings provide a strong framework to determine the impact of management practices on soil C and soil health. 相似文献
18.
R. J. Haynes 《Biology and Fertility of Soils》1999,30(3):210-216
The effects of 5 years of continuous grass/clover (Cont grass/clover) or grass (Cont grass) pasture or 5 years of annual
grass under conventional (Ann grass CT) or zero tillage (Ann grass ZT) were compared with that of 5 years of continuous barley
(LT arable) on a site which had previously been under arable crops for 11 years. For added comparison, a long-term grass/clover
pasture site (LT past) nearby was also sampled. Soil organic C (Corg) content followed the order LT arable=Ann grass CT<Ann grass ZT<Cont grass=Cont grass/clover<LTpast. Trends with treatment
for microbial biomass C (Cmic), basal respiration, flourescein diacetate (FDA) hydrolytic activity, arginine ammonification rate and the activities of
dehydrogenase, protease, histidase, acid phosphatase and arylsulphatase enzymes were broadly similar to those for Corg. For Cmic, FDA hydrolysis, arginine ammonification and the activities of histidase, acid phosphatase and arylsulphatase, the percentage
increase caused by 5 years of continuous pasture (in comparison with LT arable) was 100–180%, which was considerably greater
than that for organic C (i.e. 60%). The microbial metabolic quotient (qCO2) was higher for the two treatments which were mouldboard ploughed annually (LT arable and Ann grass CT) than for the undisturbed
sites. At the undisturbed sites, Corg declined markedly with depth (0–15 cm) and there was a similar stratification in the size and activity of Cmic and enzyme activity. The microbial quotient (Cmic/Corg) declined with depth whilst qCO2 tended to increase, reflecting a decrease in the proportion of readily available substrate with depth.
Received: 7 July 1998 相似文献
19.
Conservation tillage, and especially no-tillage, induce changes in the distribution of organic pools in the soil profile.
In long-term field experiments, marked stratification of the total soil microbial biomass and its activity have been observed
as consequence of the application of no-tillage to previously tilled soils. Our objective was to study the evolution of the
total and active soil microbial biomass and mineralized C in vitro during the first crop after the introduction of no-tillage
to an agricultural soil. The experiment was performed on a Typic Hapludoll from the Argentinean Pampa. Remaining plant residues,
total and active microbial biomass and mineralized C were determined at 0–5 cm and 5–15 cm depths, at three sampling times:
wheat tilling, silking and maturity. The introduction of no-tillage produced an accumulation of plant residues in the soil
surface layer (0–5 cm), showing stratification with depth at all sampling dates. Active microbial biomass and C mineralization
were higher under no-tillage than under conventional tillage in the top 5 cm of the profile. The total soil microbial biomass
did not differ between treatments. The active soil biomass was highly and positive correlated with plant residues (r
2=0.617;P<0.01) and with mineralized C (r
2=0.732;P<0.01). Consequently, the active microbial biomass and mineralized C reflected immediately the changes in residue management,
whereas the total microbial biomass seemed not to be an early indicator of the introduction of a new form of soil management
in our experiment.
Received: 23 February 1999 相似文献
20.
P. Perucci S. Dumontet S. A. Bufo A. Mazzatura C. Casucci 《Biology and Fertility of Soils》2000,32(1):17-23
The interactive effects of vermi-compost from sewage sludge and either the sulfonylurea herbicide, rimsulfuron, or the imidazolinone
herbicide, imazethapyr, on some soil biochemical and microbiological properties were investigated. The herbicides were applied
at field and 10-fold field rates. Both herbicides exerted a detrimental effect on soil microbial biomass and its biochemical
properties. Even though the effect of both herbicides on soil microbial biomass was not detectable at the field rate, some
significant influences on acid and alkaline phosphatase were observed. The higher rate of herbicide application impaired the
observed microbial parameters to a greater degree. The detrimental effects seemed to be reduced by organic amendments. Among
the studied microbial characteristics, the specific respiration quotient was particularly reliable and sensitive in determining
the influence of herbicides on the soil microbial biomass. In this paper a new synthetic index, specific hydrolytic activity
(qFDA), for assessing microbial activity in reply to xenobiotic treatments is proposed.
Received: 31 May 1999 相似文献