共查询到18条相似文献,搜索用时 140 毫秒
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3种莎草属伴生杂草提取物对水稻种子萌发和幼苗生长的影响 总被引:1,自引:0,他引:1
通过室内生物测定的方法就异型莎草、扁穗莎草和褐穗莎草3种莎草属伴生杂草提取物对水稻种子萌发和幼苗生长的影响进行研究。结果表明,与对照相比,250~4 000μg/mL的3种伴生杂草提取物对水稻种子萌发率基本不产生显著影响,但2 000~4 000μg/mL的异型莎草提取物对水稻种子发芽势产生显著抑制作用。异型莎草250~4 000μg/mL的水提物对水稻幼苗根长,1 000~4 000μg/mL的水提物对水稻幼苗苗高和鲜重以及2 000~4 000μg/mL的醇提物对水稻幼苗鲜重均产生显著抑制作用,相对应的最高抑制率分别为78.64%、39.28%、36.65%、22.65%。扁穗莎草250~4 000μg/mL的醇提物和500~4 000μg/mL的水提物对水稻幼苗根长,250~2 000μg/mL的水提物对水稻幼苗苗高以及1 000~2 000μg/mL的水提物对水稻幼苗鲜重均产生显著促进作用,相对应的最大促进率分别为83.76%、76.85%、21.14%、10.57%。褐穗莎草250~4 000μg/mL的醇提物和2 000~4 000μg/mL的水提物对水稻幼苗根长,1 000~4 000μg/mL的水提物对水稻幼苗苗高以及2 000μg/mL的水提物对水稻幼苗鲜重均产生显著促进作用,相对应的最大促进率分别为85.18%、78.22%、18.53%、14.77%。 相似文献
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目的研究刺五加叶不同部位在不同浓度下的体外抗氧化活性。方法采用清除ABTS+自由基、DPPH自由基评价法和测定还原性法,利用紫外分光光度计法测定刺五加叶的总提液、10%、30%和50%大孔吸附树脂醇洗脱部位在不同浓度下的抗氧化能力。结果刺五加叶不同部位抗氧化活性的强弱顺序为:30%部位>10%部位>总提液>50%部位,随着各部位浓度的增加,抗氧化能力增强。ABTS+自由基和DPPH自由基模型中,在浓度为0.64mg/mL时,30%的部位对ABTS+DPPH自由基模最大清除率分别达到99.541%、95.331%,与同浓度VC的清除率接近,其次浓度0.64mg/mL的10%部位的最大清除率分别达到96.324%、88.033%。在还原性法测定中30%部位在浓度为0.64mg/mL时,吸光度为2.269,与VC较接近,同浓度时10%部位的吸光度为2.155,次于30%部位。结论刺五加叶抗氧化能力较好,各种评定方法结合分析,其30%部位最好,可以开发为抗氧化剂,为进一步研究和开发刺五加叶提供了科学依据。 相似文献
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通过室内生物测定的方法研究满江红提取物对水稻种子萌发及幼苗生长的影响,并就提取物浓度与水稻生长相关指标间的关系进行回归分析。结果表明,与对照相比,250~4 000μg/mL的提取物对水稻种子萌发不产生显著影响;1 000μg/mL的醇提物和水提物分别对水稻幼苗根长和苗高产生显著促进作用,相对应的促进率为27.32%和9.15%;250~4 000μg/mL的醇提物和4 000μg/mL的水提物对水稻幼苗苗高,1 000~4 000μg/mL的水提物对水稻幼苗根长,2 000~4 000μg/mL的醇提物和4 000μg/mL的水提物对水稻幼苗单株鲜重产生显著抑制作用,最大抑制率分别为31.85%、14.28%、23.41%、16.49%和10.94%。拟合满江红醇提物浓度与水稻幼苗根长、苗高和单株鲜重的关系(根长y=-0.215x2+1.541x+5.133,p=0.001;苗高y=-0.035x2-0.062x+5.574,P<0.001;鲜重y=-0.685x2+1.484x+100.0,P<0.005)以及水提物浓度与水稻幼苗根长、苗高和单株鲜重的关系(根长y=-0.005x2-0.276x+7.012,P<0.001;苗高y=-0.123x2+0.760x+4.490,P<0.001;鲜重y=-1.742x2+10.49x+89.27,P<0.005)均以二次曲线函数较佳。 相似文献
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西洋参皂苷单体P-F11对大鼠血流动力学心室肌细胞动作电位的作用 总被引:5,自引:0,他引:5
目的 :观察西洋参单体皂苷P -F1 1 对大鼠心功能的作用。方法 :采用离体心脏灌流和培养大鼠乳鼠心室肌细胞 ,记录动作电位方法。结果 :西洋参皂苷单体P -F1 1 ,可使离体心功的左室内压 (LVSP)、心室舒末压 (LVEDP)、左室内压最大升降速率 (±dp/dtmax)、动作电位的幅值 (APA)、超射 (OS)、最大除极速度 (Vmax)和动作电位时程 (APD50 )等相关参数增加。结论 :西洋参单体皂苷P -F1 1 对心功能有正性肌力作用 ,提示 ,西洋参单体皂苷P -F1 1 可能与心室肌上的钙通道有关 相似文献
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采用实验室测定和温室盆栽方法研究了8种杀菌剂对大豆疫霉菌(Phytophthora sojae)菌丝生长和繁殖的影响及它们对大豆疫霉根腐病的接种防治效果。结果表明,50%烯酰吗啉可湿性粉剂、97%甲霜灵可湿性粉剂和58%甲霜灵锰锌可湿性粉剂对大豆疫霉菌的菌丝生长和繁殖具有很好的抑制作用,该3种药剂能够明显抑制菌丝生长,抑制中浓度(EC50)值依次为0.1208μg/mL、0.1563μg/mL和0.3603μg/mL。浓度为10.0μg/mL时,对孢子囊形成和萌发的抑制率均为100%,对卵孢子形成抑制率分别为64.5%、59.5%和55.0%。接种防治试验结果显示,以上3种药剂喷药后7d防效依次为73.4%、68.8%和60.5%,其余的5种杀菌剂防治效果相对较差。 相似文献
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建立高效液相色谱法(HPLC)检测紫菀根、茎、花中紫菀酮含量,采用甲醇20mL水浴加热40℃再经超声提取样品溶液。流动相为乙腈:水(96:4)、检测波长200nm、柱温40℃、进样量20μL、流速1.0mL/min的色谱条件下进行紫菀酮成分测定。不同浓度标准品检测获得标准曲线y=24584.32x-9013.67,R2=0.9999。重复性RSD%=0.53%。检出限0.03μg/mL。紫菀根、茎、花三部分均检出紫菀酮,紫菀酮含量根部1372.85μg/g、茎部14.94μg/g、花中16.42μg/g。精密度试验RSD=1.46%。平均回收率为101.02%。试验结果表明操作方法简单、重复性良好、检出限低、精密度良好、回收率较高。 相似文献
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Introduction: Antibiotic supplements are regularly used in neuronal culture media to control contamination; however, they can interfere with the neuronal excitability and affect electrophysiological properties. Therefore, in this study, the effect of penicillin/streptomycin supplements on the spontaneous electrophysiological activity of hippocampal pyramidal neurons was examined. Methods: Electrophysiological whole-cell patch-clamp recordings from rat hippocampal pyramidal cells in primary culture were performed to investigate the effects of antibiotic supplements on the intrinsic excitability of cultured cells. Results: The present findings indicated that presence of antibiotic supplements (penicillin/streptomycin) in the culture medium altered the intrinsic electrical activity of hippocampal pyramidal neurons in primary culture. These alterations included: 1) depolarized resting membrane potential; 2) a significant enhancement in the after-hyperpolarization amplitude; 3) a significant increase in the area under the action potential and in the decay and rise time of the action potential; 4) a significant broadening of action potential and 5) a significant reduction in the firing frequency. Conclusion: These findings suggest that addition of antibiotic supplements to culture media influences the neuronal excitability and alters the electrophysiological properties of cultured neurons, possibly through changing the ionic conductance underlying neuronal excitability. Key Words: Primary cell culture, Patch-clamp techniques, Hippocampus 相似文献
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《Plant Production Science》2013,16(4):392-397
abstractThe effects of various carbon sources, sucrose, glucose and fructose alone or in combination on the in vitro growth of banana plantlets were studied. Banana plants were cultured on the media supplemented with these carbon sources at 0.08 M for 13 weeks. The water potential of the medium was the highest in the medium supplemented with sucrose + glucose (-0.3 MPa), and was significantly lower in the medium supplemented with fructose alone or in combination with other carbon sources (-0.7 to -1.0 MPa) than in the other media. The leaf water potential was also the highest in the plants cultured on the medium supplemented with sucrose + glucose, and lowest in the plants cultured on that with fructose. The leaf water potential of plants cultured on sucrose + glucose, sucrose and glucose correlated well with their growth and photosynthetic activity, but the correlation was not observed in the plants cultured on fructose alone or in combination with other carbon sources. Plants cultured on fructose had a lower chlorophyll content (400 ptg dm-2) and lower photosynthetic rate (3 μmol02 m-2 s-1) than those cultured on sucrose + glucose (15,950 μgdm-2 for chlorophyll and 8.5 μmol02 m-2 s-1 for photosynthesis), and these differences were statistically significant. Both chlorophyll content and photosynthetic oxygen evolution were the highest in the plants cultured on sucrose + glucose, and the superior growth of plants on this medium was attributed to their high photosynthetic efficiency. 相似文献
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CAI Xiao-Ling(Dianxi Science and Technology Normal University,Lincang, Yunnan 677000) 相似文献
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晶两优534是籼型两系杂交水稻,2018年晚季引进在惠安县辋川等镇种植,表现分蘖力强、生长整齐、穗大粒多、结实率高、产量好、米质优、抗病性强,适宜惠安县晚季种植推广。总结晶两优534在惠安县辋川镇种植表现及栽培技术。 相似文献
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利用湘中地区1980—2017年逐日气象资料,结合2007—2017年湘中地区双季早稻产量资料和3个水稻气象观测站的观测数据,采用M-K突变分析了湘中地区双季早稻播种适宜期调整,同时通过灰色关联法分析了双季早稻各生育期内农业气候因子对产量的影响,筛选出4个主要农业气候指标,并探索其适应性调整,最后利用气候指标适宜度评价模型进行验证。结果表明,湘中地区1980—2017年春播期稳定通过10℃初日的突变点在1997年,湘中地区双季早稻的播期可由上世纪90年代制定的3下旬至4月上旬,提前至3月中旬至4月初,提前约7d;通过灰色关联法和多项式法确定影响双季早稻产量的主要农业气候指标及其阈值分别是:拔节至成熟期10℃以上积温≥1546.9℃、拔节至成熟期日照时数≥291.1h、秧田期10℃以上积温≥476.8℃和移栽至分蘖期平均气温≥19.8℃;最后气候指标适宜度评价模型验证结果表明,这些农业气候指标适宜应用到湘中地区双季早稻的实际生产中。本研究结果为湘中地区双季早稻气候指标体系的建立和更新、产量预报和种植制度的调整提供了理论支撑。 相似文献
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