EFFECT OF SPINAL FLUID FROM PATIENTS WITH MYASTHENIA GRAVIS ON THE SYNTHESIS OF ACETYLCHOLINE IN VITRO

Human spinal fluid is a more favorable medium to further the synthesis of acetylcholine in vitro, using enzyme obtained from frog brain, than is serum. Also, since less acetylcholine was synthesized in the presence of spinal fluid from patients with myasthenia gravis than spinal fluid of control subjects, it is probable that at least some of the factors responsible for the decrease and increase of the synthesis of acetylcholine pass into the spinal fluid.     

AGGLUTINATION OF STAPHYLOCOCCUS AUREUS IN THE PRESENCE OF CHORIOALLANTOIC FLUID FROM HEN'S EGGS

Certain strains of Staphylococcus aureus will agglutinate when grown in the presence of normal or influenza virus-infected chorio-allantoic fluid. Fluids are rendered inactive after heating at 56 degrees C. for one or two minutes. Surface tension and temperature are not important factors in the manifestation of the phenomenon so long as they are within the limits compatible with growth. The reaction is favored by an alkaline pH. Bacterial dissociation is not a feature of this phenomenon and the relationship of this reaction to bacteriophagy is probably not significant but is being investigated further. Similar reactions as described for the egg fluids uiay be seen when Berkefeld filtrates of influenza virus-infected and normal mouse lungs are used. A more detailed account of all the data will appear at a later date.     

超临界流体萃取红芝三萜化合物

采用超临界流体设备,以ＣＯ２为萃取剂,从野生红芝子实体中萃取灵芝三萜化合物。分析了ＣＯ２密度、温度、时间及改性剂（甲醇）对红芝三萜化合物萃取的影响。     

水稻-固氮粪产碱菌内根际体系的探讨

水稻接种固氮粪产碱菌后,可清晰看到细菌紧密地附着在稻根的表面。扫描电镜观察结果表明:其主要分布区域为根系的伸长区和根毛区,根尖端分生区内侧较少。从冰冻断裂的根横剖面中发现,进入稻根内的细菌成蔟地存活于外皮层、皮层大通气腔、直至中柱维管组织中。大约有0.01%-0.1%附着于根表的粪产碱菌进入根内,多数存在于细胞间隙中,少量进入根细胞内,在根内的粪产碱菌仍可以繁殖。粪产碱菌进入根内的途径可能是通过根表皮细胞间隙和根毛基部。粪产碱菌同样也存在于淹水茎内。接种量的多少与菌在水稻植株中的数量、水稻的长势及植株重量呈正相关。与整体根系相比,研碎后的根系具有较高的乙炔还原活性,且根系的乙炔还原活性显著高于茎段的活性。     

CO2超临界流体萃取芦笋中熊果酸的研究

用CO2超临界流体萃取得到的芦笋(Asparagus officinalis L.)提取物，用甲醇抽提醇溶部分，经用液质联用仪检测，得到56个紫外检测峰。其中，有保留时间与熊果酸基本一致的峰。其质谱的分子离子峰及特征碎片峰也与熊果酸的一致。确定该化合物为熊果酸。     

中国水牛脊髓颈膨大部灰质结构的特征

本研究通过８头初生中国水牛脊髓灰质细胞构筑的观察，提出中国水牛脊髓颈膨大部灰质结构的特征，并确定中国水牛颈膨大部准确的位置在Ｃ５－Ｔ２，这与印度水牛及其他动物的情况有所不同，讨论了有关脊髓灰质结构特征与颈膨大位置之间的关系。     

固氮粪产碱菌与宿主水稻的相互作用

本工作采用不同方法如光学、荧光、电子显微镜、#+（10）B-α径迹法等研究了粪产碱菌对水稻根的侵染能力和它们在稻根中的分布规律,并通过稻根单细胞和原生质体的电镜观察,进一步确证了粪产碱菌聚集并紧密地附着在水稻根表,但部分细菌可以进入稻根细胞内。应用组织培养技术获得了粪产碱菌与稻根愈伤组织的联合共生体。#+（15）N#-2示踪技术表明,二者具有较紧密的联合共生关系。粪产碱菌从愈伤组织提供的碳源中获得能量固定分子氮,并能提供维持愈伤组织生长所需的氮源。其最大固氮量为35.53μgN/克（干重）·天。