新疆家蚕血液蛋白质含量与茧质性状的相关性分析

本文以家蚕五龄期血液为材料,采用考马斯亮蓝G-250法,对20份基础家蚕品种材料的血液蛋白质含量进行测试并对茧质间的相关性进行了分析。分析得出:(1)各品种材料间的血液蛋白质含量有着显著差异,高的为1387．86 ug/mL,低的为666．22 ug/ mL;(2)家蚕血液蛋白质含量与全茧量、茧层量、茧层率等经济性状,在一定的范围内,有着一定的相关性,其中与茧层率之间的正相关性大于茧层量;与茧层量间的相关性大于全茧量;(3)家蚕血液蛋白质含量的测定,可以预测家蚕品种茧质遗传基因质量性状的环境表达及相对稳定性,在高茧层率品种的亲本选配和系统选育领域上有一定的借鉴及参考价值。     

家蚕血液超氧化物歧化酶遗传性及其与经济性状相关性研究

家蚕血液SOD具有相当高的遗传力,SOD活性与幼虫龄期经过无相关性,与同宫率、茧层率无明显相关,与虫蛹率、幼虫生命率、全茧量、茧层量、普茧率有一定程度负相关,与万蚕产茧量、万蚕茧层量有紧密的负相关,认为家蚕血液SOD活性与育种上要求的经济性状存在一定的矛盾.     

对我所10个家蚕高茧层率基础品种的茧质稳定性分析

采用Eberthart的品种稳定性分析方法,对我所选育的10个高茧层率基础品种进行了全茧量,茧层量,茧层率的稳定性分析,结果表明茧质性状的遗传稳定性受环境的影响最大,而受品种及品种与环境互作间的影响相对较小.品种124,125,141综合性状稳定,作为蚕育种的基础材料有一定的利用价值.     

家蚕基础品种的茧质稳定性分析

采用Eberthart的品种稳定性分析方法 ,对我所选育的 1 0个高茧层率基础品种进行了全茧量 ,茧层量 ,茧层率的稳定性分析 ,结果表明茧质性状的遗传稳定性受环境的影响最大 ,而受品种及品种与环境互作间的影响相对较小。品种 1 2 4,1 2 5,1 4 1综合性状稳定 ,作为蚕育种的基础材料有一定的利用价值。     

家蚕部分数量性状的遗传效应分析

本文以二化性品种为材料,采用不完全双列杂交法分析家蚕部分数量性状的遗传效应.分析得知:家蚕的幼虫生命率、虫蛹率、万蚕收茧量、全茧量、茧层量等性状具有显性效应,无上位性效应;茧层率性状既具有显性效应又具有上位性效应.系统间遗传分量和基因频率有的性状较一致,有的性状差异较大.     

家蚕茧质性状基因型—环境互作效应的研究

对 3个非减数分裂孤雌生殖蚕品种的 3个茧质性状数据进行了方差分析 ,并建立起一个包括微环境效应在内的基因型—环境互作生物统计模型 ,对该模型的参数作最大似然估计认为 :在家蚕的全茧量、茧层量和茧层率3个茧质性状中 ,除独立的基因型和环境效应外 ,基因型与环境的互作效应也是影响家蚕茧质性状的表现型的重要因素 ,而且这种互作效应有时要大于独立的基因型效应 ,即在茧质性状的表现型中基因型与微环境的交互作用非常重要。     

家蚕部分品种的茧质稳定性调查

运用回归分析法,对部分纯系家蚕品种的茧质性状进行了稳定性测定,结果表明,品种782全茧量、茧层量、茧层率性状表现稳定,在杂交育种中有重要的实用价值.     

茧层量差异家蚕品种后部丝腺细胞和血液组织的蛋白质表达谱分析

为了研究家蚕茧层量性状的分子调控机制,采用蛋白质双向电泳和图像分析技术,分析了遗传背景基本相同,而结茧性状和茧层量具有明显差异的正常茧、薄皮茧、裸蛹3个家蚕品种的丝腺细胞和血液组织的蛋白质表达与茧层量的关系。结果表明:裸蛹品种的丝腺细胞蛋白斑点与正常茧品种的蛋白斑点的匹配率仅在65%左右,且存在35%左右的特异蛋白斑点,暗示裸蛹性状可能是主效基因控制的微效多基因遗传;3个品种后部丝腺细胞特异蛋白斑点、匹配蛋白斑点按等电点划分的分布规律具有明显差异,而血液组织相应的分布规律非常相似,暗示丝腺细胞中的蛋白及其基因对家蚕茧层量性状的作用要大于血液组织中的蛋白及其基因;正常茧、薄皮茧和裸蛹3个品种后部丝腺细胞中特异蛋白斑点及上调和下调蛋白斑点中,可能分别存在着与家蚕高茧层量性状、结薄皮茧性状以及不结茧性状有关的蛋白质。     

定量给桑对18个家蚕纯种茧质及健康指数的影响

为了选择茧层率高和其它性状优良的品种用作育种材料,于2015-2016年春、秋蚕期4次分别以我所保育的18个品种为研究对象,采取定量给桑、个体选择等方式开展比较试验。结果表明:18个品种的结茧率、虫蛹率、全茧量、茧层量、茧层率、万蚕产茧量存在明显的差异,茧层率表现优良的品种是育成高茧层率品种的优选材料,而综合性状优良的品种则是选育适应性广的优选材料。     

3个家蚕品种部分产茧量及体质量性状指标的协方差分析

养蚕生产中家蚕的桑叶食下量难以人为控制在同一水平,通常认为食下量会对蚕体生长发育及全茧量等产茧量指标产生显著甚至极显著影响。以山东省现行的3个春用蚕品种为材料,将食下量作为协变量进行协方差分析的结果表明,3个家蚕品种在3-5龄的蚕体质量增量、全茧量、茧层量性状指标上无统计学意义(P>0.125 2),仅个别指标均值间的最大差异在0.1水平下显著(P全茧量=0.066 9,P茧层量=0.056 1),因此该方法校正了用常规方差分析得出的上述性状指标在品种间存在极显著差异(P<0.000 1)的有偏结论,还明确了消化吸收量对上述性状指标影响不显著,可不作为协变量考虑。     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

Comparison of 2 methods of centesis of the bursa of the biceps brachii tendon of horses

REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.     

不同样本商品蜂蜜中硒含量的测定

用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10～0.82μg/g。表明:蜂蜜应视为天然富硒营养品。     

乳酸杆菌益生作用机制的研究进展

乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分（表面蛋白、脂磷壁酸和肽聚糖）与肠道受体（Ｃ型凝集素受体、Ｔｏｌｌ样受体和 Ｎｏｄ样受体）,阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。     

中华人民共和国农业部公告 第267号

为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …     

鸡败血支原体垂直传播模式及其阻断方法

以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.